Book Plant Sympo 19 en

Plants with health benefits and Biomolecules of Interest University of Bordeaux 27 – 29 Nov 2019
2nd Indian-French Symposium

Plants with health benefits and Biomolecules of interest
From the plantlets to the tablets
Phyto-Health
University of Bordeaux
Agora du Haut Carré, Talence Campus France
27th – 29th November, 2019
https://plant-sympo-19.sciencesconf.org/
International Week
1
Acknowledgment
This event is an initiative of Synadiet and the University of Bordeaux. It was possible
thanks to the help and contribution of many different people and services.
Synadiet made a large contribution helping in the building of the program. It was also crucial
for the diffusion of the event to the corporates of the Dietary Supplement Industry.
Among other stakeholders we want first to thank the French Embassy in India (Delhi) and
specifically the person in charge of Research and Education partnerships: Mrs Adele Martial-
Gros. We also want to thank the French Consul in Bangalore Jérôme Bové. Without him we
would have had the opportunity to meet the Scientists from the University of Hyderabad.
We also want to thank our Indian colleagues from the University of Hyderabad and other
places in India for their trust in this collaborative project. Particularly, we are grateful to the
Vice Chancellor Pr Appa Rao Podile, the director of International Affairs in UoH: Pr N Siva
Kumar and also to Pr Sarada N Tetali for their help in many circumstances. We will try to
remain worthy of this confidence in the future.
In Bordeaux we want to thank the International Affair service for its help in the creation and
management of this event. Beyond the service itself we want to thank the persons, Pierre-Yves
Tourpin, Frédéric Bertrand, Pedro Santiago and Véronique Debord-Lazarro. We also want to
thank the Vice Presidents of International Affairs for their trust in this project Pr Joanne Pagèze
and Pr Laurent Servant. Besides, no agreement could have been signed without the work of
the Legal Affair service.
This event was possible thank to financial supports coming from different organs and institutes
of the University of Bordeaux.
1. An Idex funding attributed by the Research council of the University of Bordeaux
2. A support coming from the Department of Environmental Sciences
3. Financial help by the Labex Cote and by the scientific network MetaboHUB
We also get funds from public organisations
1. The French Embassy in India
2. The international service of Bordeaux Metropole
3. The New Aquitain Region
Pileje Pharmaceutical Laboratory always showed a great concern for the quality of its products
working with scientists and medical doctors as much as possible. Pileje chose to sponsor this
event and we are grateful to them.
Last but not least we want to thank all the colleagues in Bordeaux and elsewhere that accepted
to help in creating this program: Pierre Petriacq, Josep Valls-Fonayet, Michel Hernould,
Dominique Rolin, Ambroise Martin, Boutayna Frih, Christelle Chapteuil, Catherine Bennetau:
the dream team that visited Hyderabad in February 2019.
2
Planning
08:00 am: Registration opening 27th November 2019
Wednesday 27th of November 09:00 am
09:00 am – 01:00 pm: Academic Session: The next steps towards enhanced win-win collaborations
09:00 am – 10:00 am: Introduction and presentation of the different Institutes Vice President Pr Joanne Pagèze
– Vice-Chancellor UoH Pr Appa Rao Podile
10:00 am - 11:00 am: Presentation of Indian Masters and PhD programs in Hyderabad UoH International
director Pr N. Siva Kumar
11:00 am – 11:30 am Pause/Poster session
11:30 am – 12:30 am: How to increase partnerships between UoH and UB (Master & PhD) UB Master
directors and International mobility facilities and join PhD (especially join supervision) Dr Stephanie Danaux
12:30 am – 01:00 pm: MoU signature
LUNCH 01:00 pm – 02:00 pm

02:00 pm - 02:30 pm: Welcome Christelle Chapteuil & Catherine Bennetau
02:30 pm -04:30 pm: Session 1: Conventional vs organic production of plants with health benefits
Chairwomen: Christelle Chapteuil (Juvamine), Céline Franc (ISVV UB),
02:30 pm – 03:00 pm: Plenary lecture: Pesticide Analysis in Food Ir Céline Franc (ISVV, UB)
03:00 pm – 03:30 pm: Development of Stevia culture in France Dr Valérie Shurdi-Levraud (UB)
03:30 pm – 04:00 pm: Importance of plant production in plant quality Denis Bellenot (Iteipmai)
04:00 pm – 04:30 pm: Production of active molecules from plants in vitro Pr. Padmaja Gudipalli (UoH)
4:30 pm -5:00 pm Pause / Poster session
5:00 pm – 6:30 pm: Session 2: Vegetal extraction for quantification and production Chairmen: Christophe
Ripoll (CEO Natinov), Josep Valls (MIB, ISVV, UB)
05:00 pm – 05:30 pm: Characterization of a Tumor-Specific Lectin from Mulberry Pr Musti J Swamy (UoH)
05:30 pm – 06:00 pm: Preparative Extraction at laboratory scale Dr Josep Valls (MIB, ISVV, UB)
06:00 pm – 06:30 pm: Extraction of pesticides for quality control Dr Marie-Hélène Devier (LPTC, EPOC, UB)
Thursday 28th of November 2019 – 8:00 am

08:00 am – 10:00 am: Session 3: Which objectives for the use of metabolomics of plant-based products
Chairmen: Dr Pierre Petriacq (UB), Dr Constanze Mueller (Helmholtz Zentrum Munich - HMGU)
08:00 am – 08:30 am: Plenary lecture: New Bioactives & HCS approaches combined with Metabolomics Dr
Constanze Mueller (HMGU).)
08:30 am – 09:00 am: Metabolomic in desert plants Thomas Dussarrat (BFP, EGFV)
09:00 am – 09:30 am: Plant metabolomics: A tool for authentication of medicinal plant products Pr Sarada
Tetali (UoH)
09:30 am – 10:00 am: Metabolomic to analyse plant metabolites Pr Bertrand Gakière (Univ Paris Sud)
10:00 am – 10:30 am Pause / Poster session
10:30 am – 12:30 am: Session 4: Formulation and interaction Chairwomen: Pr Catherine Bennetau (UB),
Pr Sarada Tetali (UoH)
10:30 am – 11:00 am: Plenary lecture: Plants & drug interactions Dr François Petitet (Herbéo)
11:00 am – 11:30 am: Therapeutic biomolecules from plants with anticancer properties. Dr. Irfan Ghazi (UoH)
3
Planning
11:30 am – 12:00 pm: Plant antioxidants and peripheral clock during aging Pr Anita Jagota (UoH)
12:00 pm – 12:30 pm: Food supplement formulation Dr Sophie Lafay (Jenwin, Gynov)
Lunch 12:30 pm - 02:00 pm

02:00 pm – 02:30 pm: Funding opportunities between France and India Cécile Bacles (UB)
02:30 pm – 05:00 pm: Session 5: Quality control in food supplements Chairwomen: Dr Boutayna Frih (UB)
Pr Joana Amaral (Univ Lisboa)
02:30 pm – 03:00 pm: Plenary lecture: Quality control of food supplement Pr Joana Amaral (Univ Lisboa)
03:00 pm – 03:30 pm: Metabolite based evaluation and quality control of spices Pr Agepati Raghavendra (UoH)
03:30 pm – 04:00 pm: Unexpected role of glutathione in the oxidative transformation of flavan-3-ol by
anthocyanidin synthase in Vitis vinifera Pr Jean Chaudière (UB)
04:00 pm – 04:30 pm: Analysis of plant extracts quality in industrial settings Lucile Berthomier (Pilèje)
04:30 pm – 05 00 pm: Plants quality control Loïc Loffredo (BotaniCERT)
Departure for Social Event 05:30 pm

06:00 pm Social Event Visit of Chateau Couhins (Pessac Léognan) and diner
Friday 29th of November 2019 08:30 am

08:30 am – 10:30 am: Session 6: Regulation and Quality control Chairmen: Pr Ambroise Martin, Pr N Siva
Kumar (UoH)
08:30 am – 09:30 am: Plenary lecture: European and French regulation on health claims Pr Ambroise Martin
(Univ Lyon)
09:30 am – 10:00 am: The Canadian regulation with the Health Natural products Emilie Chauvigné (Valbiotis)
10:00 am – 10:30 am: Nutrivigilance system Aymeric Dopter (Anses)
10:30 am – 11:00 am Pause / Poster award ceremony
11:00 am – 12:30 am: Session 7: Selling and opportunities Chairmen: Christelle Chapteuil (Juvamine),
Christophe Ripoll (Natinov)
11:00 am – 11:30 am: Plenary lecture: French and European Market Christelle Chapteuil (Juvamine)
11:30 am – 12:00 pm: Indian and French supply chains of plants for health, beauty and well-being Christophe
Ripoll (Natinov)
12:00 pm – 12:30 pm: The Nagoya Protocol on Access and Benefit-sharing. Pr Zoubida Charrouf (Univ
Mohamed V Rabat)
Lunch 12:30 am - 01:30 pm

01:30 pm – 03:30 pm Round table mixing Industrial, Practitioners and Scientists
01:30 pm – 03:30 pm: Round Table from the Plantlets to the Tablets Dr Philippe Millet (Edition BGM)
- Prescription of dietary supplement (MD, M. Tourasse Hippocratus)
- The place of food supplements in the health system, Ugo Feracci & Donatien Gamba (Frost & Sullivan)
- Pharmacognosy and Food supplement quality Dr Caroline Rouger (MIB, ISVV, UB)
- Quality Control of Indian Medicinal Plant Products, Pr Sarada Tetali (UoH)
03:30 pm – 04:00 pm Pause/Transportation
04:00 pm – 06:00 pm: Platform visit CGFB facilities
4
Table of contents
Cover page.pdf 1
acknowledgements 2.pdf 2
PLanning Printed.pdf 3
Master Track Plants with health benefits and biomolecules of interest, Catherine
Bennetau [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
An Erasmus Mundus accreditation for the Environmental Contamination and

Toxicology Master program, Nathalie Geneste [et al.] . . . . . . . . . . . . . . . . 6
Production of active molecules from plants in vitro, Padmaja Gudipalli [et al.] . 8
Pesticide analysis in food, Céline Franc [et al.] . . . . . . . . . . . . . . . . . . . . 9
Main Factors influencing the quality of herbal medicinal products, Denis Bellenot 10
Screening for Stevia rebaudiana response to Septoria Leaf Blotch in controlled

conditions, Zoé Le Bihan [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
The TomGEM project: Effects of elevated temperatures on floral meristem initi-

ation and reproductive organ development in Tomato, Frederic Delmas [et al.] . . 12
Evaluation of Stevia rebaudiana Genotypes under Field Conditions in the South

west of France for Organic Production Establishment, Cécile Hastoy [et al.] . . . 13
Development of Stevia rebaudiana production in Europe: Germplasm evaluation

and molecular markers development for breeding purposes, Cécile Hastoy [et al.] 14
Encapsulation of trans--viniferin in multi-lamellar liposomes, Pauline Beaumont [et

al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
1
Preparative extraction in the Laboratory, Josep Valls Fonayet [et al.] . . . . . . . 17
Phenolic compounds from grapevine leaves: methods of extraction and analy-

sis, Kenza Djemaa-Landri [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Composition and antioxidant activities of stilbenes cane extracts from different

Vitis species, Grégoire Loupit [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . 20
Identification of oligomeric stilbenes produced by oxidative coupling of resveratrol

in wine and evaluation of their anti-inflammatory activity, Toni El Khawand [et al.] 22
Multipolar approach to decipher Glycosyl Inositol Phosphoryl Ceramides’ (GIPCs)

properties in plasma membrane nanodomain formation using biophysical tools, Adi-
ilah Mamode Cassim [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Oligomeric stilbenes produced by oxidative coupling of resveratrol show high

anti-fungal activity against Plasmopara viticola and Botrytis cinerea, Toni El
Khawand [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Plant metabolomics: A tool for authentication of medicinal plant products, Sarada

Tetali [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
Exploring metabolome in extremophile plants from Atacama desert, Thomas Dus-

sarrat [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
New Bioactives & HCS approaches combined with Metabolomics, Constanze Mueller 28
Metabolomics to exploit the primed immune system of tomato fruit, Estrella

Luna [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
MetaboHUB-Bordeaux Facility, Stéphane Bernillon [et al.] . . . . . . . . . . . . . 31
Therapeutic biomolecules from plants with anticancer properties, Irfan Ahmad

Ghazi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Plant-based food supplement formulation in Europe, Sophie Lafay . . . . . . . . 33
The mechanism of plants & drug interactions, François Petitet . . . . . . . . . . 34
Age induced Circadian Rhythm dysfunction in Central and Peripheral Clocks :

Therapeutic Interventions using Herbal Antioxidants, Anita Jagota . . . . . . . . 35
Metabolite based evaluation and quality control of spices, Agepati Raghaven-

dra [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
The French Nutrivigilance scheme, Aymeric Dopter . . . . . . . . . . . . . . . . . 37
2
Analysis of plant extracts quality in industrial settings, Lucile Berthomier [et al.] 38
DNA-based approaches for the botanical authentication of medicinal plants and

plant food supplements, Joana Amaral . . . . . . . . . . . . . . . . . . . . . . . . 39
1H NMR versus UHPLC-DAD-MS chemical profiling of red yeast rice dietary

supplements and quantification of monacolins, Rabab Hachem [et al.] . . . . . . . 40
Evaluation of low-field proton NMR spectroscopy for quality control of cinnamon

samples, Nao Wu [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
High-field and low-field NMR for unveiling adulteration of dietary supplements

by pharmaceutical compounds or other actives, Gaëtan Assemat [et al.] . . . . . 42
Polyphenols Biotech transfer unit, Elodie Renouf . . . . . . . . . . . . . . . . . . 43
Detection and quantification of stimulants in supplements for athletes by 1H

NMR, Stéphane Balayssac [et al.] . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Quality control of active compounds (icariins) in Epimedium grandiflorum herbal

supplements by HPLC-DAD and Mass Spectrometry, Saı̈da Danoun [et al.] . . . 45
Quantitative analysis of wine composition applied to Bordeaux red wines by 1H-

NMR metabolomics, Ines Le Mao [et al.] . . . . . . . . . . . . . . . . . . . . . . . 46
European and French regulations on claims, Ambroise Martin . . . . . . . . . . . 47
The Canadian Regulation - Natural Health Products, Emilie Chauvigné . . . . . 48
Food Supplements and Natural Health Products: A new health approach as part
of a health prevention policy., Christelle Chapteuil . . . . . . . . . . . . . . . . . 49
Impact analysis of healthcare cost savings thanks to the use of targeted dietary
supplements in France, Ugo Feracci [et al.] . . . . . . . . . . . . . . . . . . . . . . 50
Pharmacognosy and safety of food supplements, Caroline Rouger [et al.] . . . . . 51
The place of food supplements and medicinal plants in the health system, Michel
Tourrasse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Author Index 52
list of participants.pdf 55
3
Master Track Plants with health benefits
and biomolecules of interest
∗ 1
Catherine Bennetau , Cecile Cabasson 2 , Jean-Michel Merillon 3 ,
Michel Hernould 2
1
Acides Nucléiques : Régulations Naturelle et Artificielle – Université de Bordeaux, Institut National
de la Santé et de la Recherche Médicale : U1212, Centre National de la Recherche Scientifique :
UMR5320 – Faculté de Pharmacie, 146, Rue Léo Saignat, 33077 Bordeaux, France
2
Biologie du fruit et pathologie – Institut National de la Recherche Agronomique : UMR1332,
Université de Bordeaux (Bordeaux, France) – Centre INRA Bordeaux-Aquitaine 71 avenue Bourlaux
BP81 F-33883 Villenave dÓrnon, France
3
Institut des Sciences de la Vigne et du Vin (ISVV) – Université de Bordeaux (Bordeaux, France),
Institut national de la recherche agronomique (INRA) : UA4577 – Equipe Molécules d’Intérêt
Biologique (Gesvab) Unité de recherche Oenologie EA 4577, USC 1366 INRA ISVV - CS 50008 - 210,
Chemin de Leysotte - F-33882 Villenave d’Ornon (France), France
The Master course named Plants with health benefits and biomolecules of interest,
aims at training academic students from Bordeaux University and from other locations to the
scientific and regulation basis that are required for good quality dietary supplements.
The track is one of 6 included into the Master Bordeaux Biology Agrosciences. The tracks
are: Plant biotechnology; Plant breeding; Phyto and myco-pathology; Food industry and inno-
vation, Plants with health benefits and biomolecules of interest, Agrobiomedical.
The Plant with health benefits and biomolecules of interest, proposes 6 specific training-units
that focus on different issues: Agronomy of plants with health benefits; Development of plant
active substances; Food supplements: safety, efficiency, innovation; Food supplements: physio-
logical & medical approaches; Theoretical phytochemistry; Practical phytochemistry.
Among these training-units, 3 are open to continuing education as e-learning courses. Therefore,
the students from Bordeaux University are mixed with students from all over the world and the
teaching is proposed in two languages: English or French. These professionals should have at
least a bachelor level or at least a 5-years experience in the area of food supplements. They
can be plant producers, extractors, food supplements suppliers, health practitioners including
medical doctors, pharmacists, dieticians, nurses...
The courses are available on the Moodle e-learning platform from the University of Bordeaux.
Students can follow conferences that are recorded for them. They are involved in all distance
collaborative projects that are shared at the end of the course. They also have to perform indi-
vidual work for their assessment. Finally an examination is organized either in Bordeaux or at
distance using video tools.
The teaching is provided by professors, scientists, experts but also by health practitioners and
by corporate from food supplement companies. It aims at creating networks around the issue
of high quality food supplements.
∗
Speaker
4
Keywords: Master, Plants with health benefits, e, learning, International, Continuing Education
5
An Erasmus Mundus accreditation for the
Environmental Contamination and
Toxicology Master program
∗† 1
Nathalie Geneste , Agnès Feurtet-Mazel‡ 2
1
Université de Bordeaux – UMR 5805 EPOC – 35, place Pey Berland - 33076 Bordeaux, France
2
Université de Bordeaux – UMR 5805 EPOC – 35, place Pey Berland - 33076 Bordeaux, France
Launching in September 2020, the Environmental Contamination and Toxicology

(ECT+) Erasmus Mundus Joint Master Degree is a multidisciplinary and international
2 year course, taught entirely in English. ECT+ provides postgraduate training in academic
excellence in the fields of ecotoxicology, toxicology and environmental chemistry, with
solid conceptual foundations and innovative practical components based on a translational ap-
proach.
Specializing in understanding interactions between chemical pollution, the contamina-
tion of living organisms and ecosystem disturbances, ECT+ also focuses on human and
environmental health risk management, in relation to the use and disposal of chemicals in a
scenario of global change.
Students benefit from advanced knowledge in a variety of disciplines such as analytical and
environmental chemistry, ecotoxicology, cellular and molecular biology, global chemical issues,
ecology, ecosystem health assessment, environmental policies and risk management, in accor-
dance with European treaties, conventions and directives.
The program has been developed by a consortium of six leading European universities in the
domains of ecotoxicology and environmental chemistry from 4 different countries, coordinated
by the University of the Basque Country (Spain), in partnership with the Norwegian University
of Science and Technology (Norway), the University of Porto (Portugal), the University of Pau
and Pays de l’Adour (France), the University of Liège (Belgium) and the University of Bordeaux,
with the invaluable contribution of a global network of associates.
The curriculum, which is completely adapted to the requirements of the current international job
market, responds to an increasing demand for graduates who can take a leading role in various
environmental sectors. These sectors include research, ecotoxicology, environmental protection
and management, within both the chemical industry and local and global non-governmental
organizations.
ECT+ ensures that graduates are specialists in the analysis of environmental contamination
issues, field measurements, experiments, laboratory tests and modelling, as well as environmen-
tal program development.
Visit our website: http://www.ectplus.eu
∗
Speaker
†
Corresponding author: [email protected]
‡
6
Keywords: Environmental, Chemistry, Contamination, Toxicology, Ecotoxicology, Pollution, Living
organisms
7
Production of active molecules from plants
in vitro
∗† 1 1
Padmaja Gudipalli , Nyanthanglo Woch
1
University of Hyderabad – Department of Plant Sciences, School of Life Sciences, University of
Hyderabad, Gachibowli, Hyderabad – 500 046, Telangana, India
Plants produce an array of secondary metabolites as a means of adaptation to environmen-

tal and climatic conditions. Several of the plant derived secondary metabolites have immense
pharmacological and nutraceutical importance. Plant tissue culture provides an alternative to
produce secondary metabolites on large scale under controlled conditions with the possibility of
accelerating fresh biomass and continuous production of high value compounds. The successful
establishment of in vitro methods leading to efficient proliferation of callus/cell suspension cul-
tures, regeneration of whole plants, and enhanced secondary metabolite production and recovery
of desired product are critical for making the process economically viable. The medicinally im-
portant plant species of interest that are difficult to multiply through conventional methods
or produce low concentration of desired compound can be rapidly propagated ensuring high
genetic similarity and control over secondary metabolite production. There are several ways
through which enhanced production of target compounds can be achieved in vitro which in-
clude manipulation of media and growth regulators, culture conditions and more importantly
by treatment with biotic and abiotic elicitors. The rapid advancements in functional genomics
and metabolomics tools have offered very good opportunities to discover the genes associated
with biosynthetic pathways of secondary metabolites using plant cell cultures synthesizing high
level of specific compound(s). The availability of improved transformation tools as well as si-
lencing and genome-editing methods have opened new exciting possibilities for the modification
of metabolic pathways of the cultured plant cells and organs resulting in great increase in pro-
duction of targeted compound(s). A specific case study of efficient callus proliferation and plant
regeneration, and enhanced production of phenolic and flavonoid compounds by elicitation of
callus cultures with jasmonic acid and salicylic acid along with identification of bioactive com-
pound from seed coats in Givotia moluccana (L.) Sreem., an economically important softwood
tree species will be presented.
Keywords: Secondary metabolites, medicinal importance, in vitro, callus cultures, elicitation, en-
hanced production
∗
Speaker
†
8
Pesticide analysis in food
∗ 1 1
Céline Franc , Gilles De Revel
1
Unité de Recherche Œnologie, EA 4577, USC 1366 INRA, ISVV, Université de Bordeaux – Université
de Bordeaux (Bordeaux, France) : EA4577, Institut national de la recherche agronomique (INRA) :
USC1366 INRA – 210 chemin de Leysotte CS50008 33882 Villenave d’Ornon, France
Pesticides, specifically plant protection products (PPPs), also known as phytopharmaceuti-

cal products, are applied in fields for the protection of plants against multiple plagues (insects,
microorganisms, weed...). Residues of these applied pesticides may remain on or in the grown
fruits and vegetables, while another part can end up in the soil or dissipate in the atmosphere.
Pesticide residues in food products (as well as in the environment) are of great concern to con-
sumers, farmers, local residents and legislators, concerns that are widely reported in the media.
Authorizations for plant protection products depend on the country and, while new PPPs are
allowed every year, others are banned. The analysis of pesticide residues in food products
consists of analysing the active substance(s) used in the formulation of PPP. This requires the
detection and quantification of authorized and prohibited active substances, i.e. several hundred
compounds. Maximum Residue Limits (MRLs) are defined for active substances in relation to
the crop in question, but there is no MRL for processed products. Nevertheless processing a
product may reduce the content of the active substance, or, on the contrary, concentrate it.
The crucial step for pesticide residue analysis in food products is the extraction step. Sev-
eral techniques are employed; however the QuEChERS (Quick Easy Cheap Effective Rugged
and Safe) extraction is mainly used especially for fruits and vegetables. The extracted com-
pounds are then separated by two complementary methods: gas and liquid chromatography,
depending on the active substance and finally detected by mass spectrometry for unequivocal
identification and quantification.
Methods for analysing pesticide residues in food products will be discussed. Quantitative data
concerning fruits and vegetables as well as wine and examples of monitoring from vine to wine
will be shown.
Keywords: pesticide residues, food, wine, analytical methods
∗
Speaker
9
Main Factors influencing the quality of
herbal medicinal products
∗ 1
Denis Bellenot
1
Institut Technique Interprofessionnel des Plantes à Parfum Médicinales et Aromatiques – Institut
Technique Interprofessionnel des Plantes à Parfum Médicinales et Aromatiques – BP 80009 Melay
49120 Chemillé-en-Anjou, France
French association created in the 80th, iteipmai carries out applied research to assist the
perfume, medicinal and aromatic plant sector. This sector being one of the most regulated and
standadized sector, many work has been done to improve the quality of the products. From
these 40 years of practical experience, some factors can be distinguished that influence more
or less the quality of herbal medcinal products. First of them are the genetic factors i.e. the
species, chemotype or cultivar. The second one relates to the agricultural management practices.
Once the variety is choosen, crop implantation, water & fertilizer inputs, harvest time & stage,
drying techniques impact the yield, quality and rentability of the crop. The improvement of
agricultural technics is based on the compromise between the best yield and the best quality.
The last factor is the analytical procedure that is used to evaluate the quality. In most of the
cases, there is no absolute methods, only relative ones, refering to national or international
standards that are set up by organisations such as ISO ou European Pharmacopoeia. For some
active principles, many different technics can be used, producing very different data for the same
sample. Using non
Some examples of these influencing factors, arising from iteipmai research or international liter-
ature will be presented.
Keywords: iteipmai, medicinal plant, quality, variability
∗
Speaker
10
Screening for Stevia rebaudiana response to
Septoria Leaf Blotch in controlled conditions
Zoé Le Bihan ∗† 1,2 , Cécile Hastoy 1,2 , Jonathan Gaudin 3 , Patrick Cosson
1
, Dominique Rolin 1 , Valérie Schurdi-Levraud 1
1
Biologie du fruit et pathologie – Université Bordeaux Segalen - Bordeaux 2, Institut National de la
Recherche Agronomique : UMR1332, Université Sciences et Technologies - Bordeaux 1 – Centre INRA
Bordeaux-Aquitaine 71 avenue Bourlaux BP81 F-33883 Villenave dÓrnon, France
2
Oviatis – Oviatis – 3, chemin de Perroutis, 47310 Estillac, France
3
Unité Mixte de Recherche en Santé Végétale (INRA/ENITA) – Institut National de la Recherche
Agronomique : UMR1065, École Nationale dÍngénieurs des Travaux Agricoles - Bordeaux, Institut des
Sciences de la Vigne et du Vin (ISVV) – ISVV - IFR103 Centre de Recherche INRA Bordeaux
Aquitaine 71, Avenue Edouard Bourleaux BP 81, 33883 Villenave dÓrnon Cede, France
For centuries, Paraguayan Indians used Stevia rebaudiana leaves as a natural acaloric source
of sweetening. Nowadays, one of the main diseases on S. rebaudiana is Septoria Leaf Blotch.
This fungal disease causes leaf chlorosis and necrosis and leads to important yield’s losses.
Septoria Leaf Blotch has been described on stevia in Japan, United States, Canada, Paraguay,
India and France. Some genetic variability has been observed in S. rebaudiana for the character
”susceptibility to septoria infection”. In France, stevia production has been implemented since
2011 but to move to economically viable organic production, pest management has to be taken
into account. The aim of this study is to screen a Stevia rebaudiana germplasm from diverse
origins through an optimized inoculation method under controlled conditions.
An inoculation protocol has been developed by pulverizing a local strain of Septoria Leaf Blotch
on 10 genotypes’ cuttings. Symptoms were evaluated by visual scorings every 4 days after
their apparition. A scan of each individual’s leaves was performed 21 days after inoculation.
Each scan was analyzed through Image J macros. Area Under the Disease Progressive Curve
(AUDPC) was calculated based on 4 visual scorings. All inoculated plants were symptomatic but
we observed a genotype-specific response against the fungus. The response of the ten genotypes
was shown to be quantitative. We could distinguish moderately susceptible genotypes and highly
susceptible ones expressing fast disease progress. Genotypes ranking observed through controlled
inoculation was confirmed through all the scoring.
A high reliable and homogeneous automated disease screening procedure in controlled conditions
for the evaluation of S. rebaudiana genotypes response to Septoria has been set up. This
screening method can be applied to our genetic collection. This preliminary study constitutes
a promising opening for a breeding program devoted to the improvement of Stevia rebaudiana
resistance against Septoria Leaf Blotch.
Keywords: S. rebaudiana, Septoria Leaf Blotch, response screening
∗
Speaker
†
11
The TomGEM project: Effects of elevated
temperatures on floral meristem initiation
and reproductive organ development in
Tomato
∗ 1
Frederic Delmas , Christian Chevalier 1 , Michel Hernould† 1
1
While we are entering a period of increasingly rapid climate change, there are strong re-
quests to design new strategies to maintain high yields of fruit and vegetables produced in harsh
temperature conditions. By using tomato as a reference fleshy fruit crop we propose to ad-
dress these requests by studying the floral meristem initiation and reproductive development
under elevated ambient temperatures in laboratory and commercial tomato varieties. Within
the TomGEM project (Genetics, Environment Management, European Union’s Horizon 2020
research and innovation program, grant agreement N◦679796, started in march 2016) our ob-
jectives are to (1) study of the cellular and molecular bases of floral meristem and reproductive
organ abortions including pollen abortion under elevated ambient temperatures, (2) Identify the
key regulators conferring improved ability for floral meristem initiation and reproductive organ
development under elevated ambient temperatures. To date, 1) a morphological and cytologi-
cal definition of developmental stages at high resolution from initiation of floral meristems to
floral maturity is in progress by analysing flower buds of different sizes from various genotypes
submitted to high ambient elevated temperatures. 2) 614 Tomato EMS-mutant families from
the INRA EMS-mutant collection (8300 EMS-mutant families) have been grown under elevated
ambient temperatures and phenotyped for flowering time and floral defects, including pollen
and floral abortion compared to wild-type plants. The temperatures for tomato pollen abortion
have been evaluated and EMS-mutants plants producing fruits at high ambient temperatures
have been isolated. The corresponding results from this study will be presented here.
Keywords: Climate change, tomato, heat stress, quality
∗
Speaker
†
12
Evaluation of Stevia rebaudiana Genotypes
under Field Conditions in the South west of
France for Organic Production
Establishment
Cécile Hastoy 1 , Zoé Le Bihan ∗† 1,2 , Patrick Cosson 2 , Eric Sclaunich 3 ,

Philippe Boutié 1 , Sebastien Cavaignac 3 , Dominique Rolin 2 , Valérie
Schurdi-Levraud 2
1
2
3
Invenio – Invenio – Domaine de la Grande Ferrade CS 20032 71 Avenue Edouard Bourlaux 33882
VILLENAVE D’ORNON Cedex, France
In order to establish an organic Stevia rebaudiana production in South West of France,

we tested 12 different commercial genotypes coming from Eustas and other providers in our
field conditions in a first year production. Plants were evaluated for biomass traits and Steviol
glycosides (SGs) content and quality. The first results showed that the first year production of
cloned genotypes was higher compared to seeds lots in our conditions. Some genotypes as ”F”
seem to be particularly interesting according to SGs yield, RebA/stevioside ratio and minor SGs
content. Some ”seeds” genotypes seem to be interesting according to minor SGs content and
quality.
Keywords: Stevia rebaudiana, genotype, field experiment, organic production
∗
Speaker
†
13
Development of Stevia rebaudiana
production in Europe: Germplasm
evaluation and molecular markers
development for breeding purposes
Cécile Hastoy 1 , Zoé Le Bihan ∗ 1,2 , Patrick Cosson 2 , Sebastien

Cavaignac 3 , Eric Sclaunich 3 , Philippe Boutié 1 , Dominique Rolin 2 ,
Valérie Schurdi-Levraud 2
1
2
3
Invenio – Invenio – Domaine de la Grande Ferrade CS 20032 71 Avenue Edouard Bourlaux 33882
VILLENAVE D’ORNON Cedex, France
Stevia rebaudiana belongs to the Asteraceae family. Its leaves can accumulate high concen-
trations (up to 30%) of different glycosides derived from the tetracyclic diterpene steviol. These
steviol glycosides have the characteristics of high sweetness and low calories. Leaf powder of
Stevia or steviol glycosides extracts are used for several years in many countries. Recently, in
2011, using RebA extract from Stevia rebaudiana leaves was allowed for use as a food additive
in EU countries. At that time, Stevia rebaudiana is mainly produced in China. But, the open-
ing of this new market leads to the development of Stevia rebaudiana production in European
countries. The main point is to be able to provide farmers with adapted cultivars. For that
purpose, we gathered Stevia rebaudiana germplasm. A collection of 15 genotypes was studied
in two environnements in the South West of France. Plants were evaluated for biomass traits
and Steviol glycosides (SGs) content and quality. This first analyse revealed the potential of
some genotypes in our field conditions. In the same time, due to a huge lack of sequence data
SSR markers were developed to classify this germplasm. The polymorphism of the 42 selected
microsatellite markers was assessed on a set of 26 genotypes from different geographical area
including commercialized genotypes and other species belonging to Stevia genus. This allowed
us to classify our genetic diversity. The development of SNP markers is ongoing to support our
breeding program.
Keywords: Stevia rebaudiana, genotype, field experiment, microsatellites
∗
Speaker
14
Encapsulation of trans--viniferin in
multi-lamellar liposomes
∗ 1 ∗
Pauline Beaumont , Claude Atgié
2 ∗
, Arnaud Courtois
1 ∗
, Stéphanie Krisa
1 ∗ † 2
, Chrystel Faure
1
Institut des Sciences de la Vigne et du Vin (ISVV) – Université de Bordeaux (Bordeaux, France) –
210 Chemin de Leysotte, 33140 Villenave-d’Ornon, France
2
Chimie et Biologie des Membranes et des Nanoobjets – Université Sciences et Technologies -
Bordeaux 1, École Nationale dÍngénieurs des Travaux Agricoles - Bordeaux, Centre National de la
Recherche Scientifique : UMR5248 – IECB 2, rue Robert Escarpit 33607 PESSAC CEDEX, France
The trans--viniferin (VNF), a resveratrol dimer is mainly provided by red wine in human
diet (0.2 to 4.3 mg/L). The antioxidant, anti-inflammatory and anti-obesity activities of this stil-
bene have been highlighted both in vitro and in vivo. Mice with high-fat diet supplemented with
VNF showed a significant decrease in body weight compare to the control group. In addition,
VNF decreased the mass of white adipose tissues. VNF has also shown in vitro anti-adipogenesis
activities on pre-adipocytes (3T3-L1) by reducing the size of lipid droplets, decreasing the ac-
cumulation of peroxisome proliferator-activated receptor gamma (PPARγ) mRNAs involved in
adipogenesis.
Although VNF has shown promising biological activities, pharmacokinetic studies have revealed
a low bioavailability of this molecule after oral administration (0.771%), mainly due to both low
absorption and high hepatic metabolism. In addition, VNF was shown to exert some cytotoxic
effects thus limiting its biocompatibility toward the cells of the intestinal epithelium. In order
to circumvent these issues, the encapsulation of VNF could represent a promising alternative to
limit VNF degradation and limit its cytotoxicity toward intestinal cells and therefore promote
its absorption.
Among the various types of encapsulation systems, onion-type multilamellar liposomes (MLLs)
were chosen since they should allow a better encapsulation efficiency. In this study, we opti-
mized their production in terms of encapsulation efficiency and characterized them by different
technics. Three methods to separate the free from the entrapped VNF forms were compared:
ultrafiltration, size exclusion chromatography and ultracentrifugation. These results allow us
to establish the simplest method for encapsulation efficiency determination, but also the most
suitable method to separate the free VNF for medium scale production. Finally, results showed
that VNF exerted cytotoxic effect toward Caco-2 intestinal cell line from 25 µM, whereas en-
capsulated VNF did not exert any cytotoxicity even for concentrations up to 100 µM.
∗
Speaker
†
15
Keywords: trans epsilon viniferin, encapsulation, multilamellar liposomes
16
Preparative extraction in the Laboratory
Josep Valls Fonayet ∗ 1 , Julien Gabaston 1 , Caroline Rouger 1 , Kenza

Djemaa-Landri 2 , Pierre Waffo-Teguo 1 , Jean-Michel Merillon 1 , Tristan
Richard 1
1
Institut des Sciences de la Vigne et du Vin [Villenave dÓrnon] – Université de Bordeaux – Unité de
recherche Oenologie, EA 4577, USC 1366 INRA - AXE Molécules à Intérêt Biologique 210, chemin de
Leysotte - CS 50008 - 33140 Villenave d’Ornon, France
2
Université de Béjaı̈a – Laboratoire de Biomathématique, Biophysique, Biochilie et Scientométrie,
Faculté des Sciences de la Nature et de la Vie, 0600 Béjaia, Algeria
The extractive procedure is a crucial step in the developments of phytochemistry labo-

ratories. The ideal extraction should assure an optimal selectivity, recovery and simplicity
and at the same time, be able to mimic the industrial procedures to facilitate a scale-up
and limit the environmental impact. In recent years, new extraction methodologies such as
Pulsed Electrical Fields, Microwave Extraction, Ultrasonication, High Pressure or Supercritical Fluid Extractio
have been developed in order to provide more eco-friendly processes. For each one of these tech-
niques, an experimental design evaluating the influence of different parameters in the efficacy
of the extraction process and the subsequent response surface analysis is the common approach
for optimizing the extraction procedure.
On the other hand, raw extracts usually are still complex materials and fractionation tech-
niques should be applied to further concentrate these matrices in the compounds of inter-
est. Membrane technologies (Ultrafiltration, Osmosis Filtration, etc) have shown a good ap-
plicability for the separation of bioactive compounds. At higher cost but with better selec-
tivities, Chromatographic techniques also are usually applied for the fractionation and iso-
lation of high amounts of the molecules of interest. In this sense, sophisticated techniques
of High Speed CounterCurrent Chromatography (HSCCC) represent very useful methodologies
thanks to the lack of solid support, which minimises adsorbance losses.
To illustrate these techniques with some examples, the utilization in our laboratory of Pres-
surized Solvent Extraction with Subcritical Water provided excellent results for the extraction
of stilbenoids from a complex matrix of grapevine roots. The application of Centrifugal Parti-
tion Chromatography (CPC) is a highly efficient technique for the enrichment of polyphenols in
plant extracts. All these methodologies assist for the discovery of new bioactive compounds and
extracts in enough quantities to perform biological studies and to be used for the development
of quantitative analytical methodologies.
Keywords: Subcritical Water, Centrifugal Partition Chromatography, Membrane technologies, Phy-

tochemistry
∗
Speaker
17
Phenolic compounds from grapevine leaves:
methods of extraction and analysis
Kenza Djemaa-Landri 1 , Marion Vu 2 , Claire Mandou 2 , Antonin Douillet

3
, Aleksandra Burzdiej 4 , Grégory Da Costa 5 , Stéphanie Cluzet 5 , Josep
Valls Fonayet ∗† 6
1
Laboratory de Biomathématique, Biophysique, Biochimie et Scientométrie, University of Bejaia –
Université de Bejaia 06000 Bejaia, Algeria
2
Université de Bordeaux Ségalen [Bordeaux 2] – Institut des Sciences de la Vigne et du Vin – 146 Rue
Léo Saignat, 33000 Bordeaux, France
3
IFV Bordeaux Aquitaine – Institut Français de la Vigne et du Vin (IFV) – 39, rue Michel Montaigne
33290 Blanquefort, France
4
Université de Varsovie – Department of Plant Biochemistry, Faculty of Biology, University of Warsaw,
ul. Miecznikowa 1, 02-096 Warsaw, Poland, Poland
5
Institut des Sciences de la Vigne et du Vin [Villenave dÓrnon] – Université de Bordeaux – 210,
chemin de Leysotte - CS 50008 - 33140 Villenave dÓrnon, France
6
Polyphenols are key ingredients in the nutraceuticals market due to their possible beneficial
effects on human health. In grapevine, polyphenols including flavonoids, stilbenes and proan-
thocyanidins can be found in different organs of the plant. In consequence, several valorization
projects of grapevine by-products as grape pomace and stems have been undertaken. In con-
trast, the valorization of grapevine leaves has been little explored, which is paradoxical, because
-unlike other waste grapevine parts- leaves are traditionally consumed as foods or used as reme-
dies in many countries. In order to confirm the richness of grapevine leaves in polyphenols and
so their potential usefulness in health-promoting products, we decided to study the extraction
and characterization of a maximum of the phenolics present in leaves of Vitis vinifera.
In our laboratory, a LC-QQQ method has been developed for the analysis of flavanols (cate-
chin, epicatechin, procyanidin dimers), flavonols (quercetin, quercetin glucoside, quercetin glu-
curonide, quercetin rutinoside, quercetin rhamnoside and myricetin) and some stilbenes (resver-
atrol, piceid, viniferins) in grapevine leaves. We have applied this methodology to study the
influence of different solvents and techniques (manual extraction, sonication, microwave extrac-
tion) on the extractability of polyphenols.
Our results allowed us to characterize the phenolic profile of grapevine leaves and better un-
derstand the choice of the best extraction for these molecules. The most abundant polyphenols
found in leaves belonged to the family of quercetin derivatives, with quercetin 3-glucuronide as
the main compound. While methanol is the best solvent of choice, good results can also be
obtained by using most suitable solvents such as hydroalcoholic mixtures with ethanol. Studies
to determine polyphenols differences between Vitis cultivars or after grapevine treatments, such
as elicitation, are currently being performed.
∗
Speaker
†
18
Keywords: quercetin, valorization, ellicitation
19
Composition and antioxidant activities of
stilbenes cane extracts from different Vitis
species
Grégoire Loupit 1 , Julien Gabaston 2 , Céline Franc 3 , Tristan Richard 2 ,

Sarah J Cookson 4 , Josep Valls Fonayet ∗ 2
1
EGFV – UMR 1287 EGFV, INRA – 210 Chemin de Leysotte, France
2
3
Institut des Sciences de la Vigne et du Vin – Institut des Sciences de la Vigne et du Vin (ISVV) – 210
Chemin de Leysotte, France
4
Ecophysiologie et Génomique Fonctionnelle de la Vigne – Institut National de la Recherche
Agronomique : UMR1287, Université de Bordeaux, Institut des Sciences de la Vigne et du Vin (ISVV),
Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine – I.S.V.V Bordeaux, 210
Chemin de Leysotte 33882 Villenave d’Ornon cedex, France, France
Resveratrol is one of the most popular antioxidant compounds used in dietary supplements
and it is also being increasingly used in cosmetology and dermatology. The attractiveness of
resveratrol is linked to its antioxidant properties and its many health-promoting activities, such
as anti-inflammation, antiageing and neuroprotective effects. Chemically, resveratrol belongs to
the family of stilbenes, which includes glucosylated derivatives (e.g. piceid), or oligomeric forms.
Dietary supplements usually consist of synthetic resveratrol or extracts from plant such as Poly-
gonum cuspidatum, which contain mainly monomers. Alternative sources could find easily a
niche in the market, since oligomerized forms show stronger antioxidant capacities. Grape canes
are thus an interesting alternative source for the extraction of these compounds, because they
represent an abundant by-product, rich in stilbenes and with high contents of oligomers. The
content and profile of stilbenes in canes depends greatly on the cultivar, and therefore detailed
composition analysis must be undertaken to select the most interesting genotypes.
In our study, we analyzed 51 different Vitis cultivars belonging to 15 different species (including
American, Asian and European) for their concentration in 24 stilbenes (together with other
significant polyphenol compounds). The results showed a high variability in the contents and
profile of stilbenes in the different species. The dimer -viniferin was the main stilbene found
in most cultivars, and the total amount varied between 0.13 - 3 g stilbenes/kg FW of cane.
Principal Coponent and cluster analysis showed differences between American and European
species. The same extracts were analyzed for their antioxidant activity, assessed by the ORAC
and DPPH tests, which showed a good correlation.
Acknowledgements:
We thank he Unité expérimentale vigne & vin bordeaux grande ferrade (UE VIGNE BOR-
DEAUX) , the technical staff of the EFGV, and MIB laboratories for their assistance.
∗
Speaker
20
Keywords: resveratrol, viniferin, DPPH, ORAC, grapevine
21
Identification of oligomeric stilbenes
produced by oxidative coupling of
resveratrol in wine and evaluation of their
anti-inflammatory activity
Toni El Khawand ∗ 1 , Josep Valls 2,3 , Iris Aja Perez 4 , Stéphanie Krisa 4 ,
Arnaud Courtois 4 , Alain Decendit 3 , Tristan Richard ∗ † 5
1
Institut des Sciences de la Vigne et du Vin [Villenave dÓrnon] – Université de Bordeaux (Bordeaux,
France) – 210, chemin de Leysotte - CS 50008 - 33140 Villenave dÓrnon - France, France
2
MetaboHUB-Bordeaux Facility – MetaboHUB-Bordeaux Facility : MetaboHUB,
PHENOME-EMPHASIS – CENTRE INRA DE NOUVELLE AQUITAINE BORDEAUX, 33 140
VILLENAVE d’ORNON CONTACT: [email protected], OC INRA 022, DOI :
10.15454/1.5572412770331912E12 https://metabolome.cgfb.u-bordeaux.fr/, France
3
4
Institut des Sciences de la Vigne et du Vin (ISVV) – Université de Bordeaux (Bordeaux, France) –
210 Chemin de Leysotte, 33140 Villenave-d’Ornon, France
5
Vitis vinifera (grapevine) stilbenes are a group of secondary metabolites found in various
parts of the plant and in grapes. Consequently, several stilbenes like piceid and resveratrol
are found in wine. Their concentration in wine can mainly vary according to the cultivar
choice and the winemaking process. However, due to their phenolic structure, stilbenes can be
altered under high temperature or oxidative conditions, leading to the formation of other stilbene
derivatives [Shingai et al., 2011]. Hence, the goal of this study is to investigate the presence
of these derivatives in a wine matrix. In attempt to obtain these compounds, we proceeded
to a hemisynthesis of stilbene derivatives from (E)-resveratrol and (E)-piceid in ethanol by
oxidative coupling using metals. The compounds formed were identified by MS and NMR
spectroscopy. Moreover, we evaluated the anti-inflammatory effects of the stilbene derivatives
by studying their ability to prevent lipopolysaccharide (LPS)-induced upregulation of NO and
cytokines production in RAW 264.7 macrophage cell line. Finally, in a preliminary study, these
compounds were identified in a wine solution model with a range of different concentrations of
piceid and resveratrol. These stilbene derivatives could serve as indicators of wine quality and
conservation conditions.
Keywords: wine, stilbenes, hemisynthesis, oxidative coupling, anti, inflammatory activity
∗
Speaker
†
22
Multipolar approach to decipher Glycosyl
Inositol Phosphoryl Ceramides’ (GIPCs)
properties in plasma membrane nanodomain
formation using biophysical tools
Adiilah Mamode Cassim 1 , Magali Deleu 2 , Laurence Lins 3 , Eric

∗
Liesnieswska 4 , Isabell Alberts 5 , Emmanuel Maes 6 , Laetitia Fouillen
7,8
, Thorstern Nuernberger 5 , Sébastien Mongrand 1
1
Laboratoire de biogenèse membranaire – CNRS : UMR5200, Université de Bordeaux (Bordeaux,
France) – Bâtiment A3 - INRA Bordeaux Aquitaine, 71 Avenue Edouard Bourlaux, CS 20032, 33140
Villenave d’Ornon, France
2
laboratoire de biophysique moléculaire aux Interfaces, Gembloux Agro Bio-tech, University of Liège –
Passage des Déportés 2, 5030 Gembloux, Belgium
3
laboratoire de biophysique moléculaire aux Interfaces, Gembloux Agro Bio-tech, University of Liège
(LBMI-GX ABT) – Passage des Déportés 2, 5030 Gembloux, Belgium
4
Laboratoire Interdisciplinaire Carnot de Bourgogne [Dijon] – Université de Technologie de
Belfort-Montbeliard, Université de Bourgogne, Centre National de la Recherche Scientifique :
UMRCNRS 6303 – Université de Bourgogne - 9 avenue Alain Savary - BP 47870 - 21078 Dijon Cedex,
France
5
Centre of Plant Molecular Biology – Eberhard-Karls-University Tubingen, Germany
6
Plateforme d’Analyse des Glycoconjugués – Université des Sciences et Technologies de Lille - Lille I –
Université de Lille, UMR8576 Avenue Mendeleiev Villeneuve d’Ascq France, France
7
Laboratoire de biogenèse membranaire (LBM) – CNRS : UMR5200, Université de Bordeaux
(Bordeaux, France) – Bâtiment A3 - INRA Bordeaux Aquitaine, 71 Avenue Edouard Bourlaux, CS
20032, 33140 Villenave d’Ornon, France
8
Plateforme MétaboHub-bordeaux – CNRS : UMR5200, Université de Bordeaux (Bordeaux, France) –
Centre INRA de Bordeaux-Aquitaine, IBVM, CS 20032, 33 140 Villenave d’Ornon, France
Plant plasma membrane (PM) lipid composition’s specificity relies on the structure and
abundance of sphingolipids and sterols compared to animal plasma membrane. While glyc-
erophospholipids are conserved in both animal and plant PM, sphingolipids and sterols chem-
istry deeply differ between the two kingdoms. Glycosyl Inositol Phosphoryl Ceramides (GIPCs)
is one of the earliest classes of plant sphingolipids discovered in the 1950’s but poorly char-
acterized due to its high polarity and low solubility in lipid extraction solvents. GIPCs are
clade-dependent structurally diverse molecules1, almost exclusively located in the outer leaflet
of the plant plasma membrane representing almost 50% of total outer leaflet lipids2. They
are receptors to necrotic toxins synthesized by deadly pathogen like mildew3. They contain
an alpha-hydroxylated very long fatty acid chain (up to 26 carbons) which likely interdigitate
with unsaturated inner leaflet lipids forming an interleaflet continuum4. They also interact with
sterols to form nanodomains which are signaling plateforms2. Biophysical techniques constitute
a powerful tool to study the interactions between lipids in protein-free membrane models. By
means of liposomes, environment-sensitive probes, Langmuir monolayers, neutron reflectivity
and solid-state NMR studies, my thesis project aims at purifying GIPCs (not commercially
available) from different plant species and at challenging the role of GIPCs through its interac-
tion with sterols and unsaturated phospholipids in PM organization and the formation of PM
∗
Speaker
23
nanodomains. Atomic Force Microscopy is also used as it gives exceptional resolution of the anal-
ysed membrane model while simultaneously acquiring information of the sample’s mechanical
properties, as we investigate lipid asymmetry in mono- and bilayer lipid model. Complementary
molecular modeling approaches highlight the behaviour of GIPCs in lipid membrane in silica
Keywords: Glycosyl Inositol Phosphoryl Ceramides, plasma membrane
24
Oligomeric stilbenes produced by oxidative
coupling of resveratrol show high anti-fungal
activity against Plasmopara viticola and
Botrytis cinerea
Toni El Khawand ∗ 1 , Julien Gabaston 2 , David Taillis 3 , Marie-Laure

Iglesias 3 , Eric Pédrot 3 , Josep Valls 3,4 , Jean-Michel Merillon 2 , Alain
Decendit 3 , Stéphanie Cluzet 3 , Tristan Richard ∗ † 3
1
Institut des Sciences de la Vigne et du Vin [Villenave dÓrnon] – Université de Bordeaux (Bordeaux,
France) – 210, chemin de Leysotte - CS 50008 - 33140 Villenave dÓrnon - France, France
2
3
4
Vitis vinifera (grapevine) stilbenes are a group of polyphenols produced in various parts of
the plant and identified as phytoalexins. Consequently, they contribute to the defense mecha-
nisms of the plant under stress conditions such as fungal attacks. In grapevine culture, these
attacks represent a serious threat for the plant health, yields and hence for the wine produc-
tion and quality. Several studies showed that grapevine stilbene extracts have high antifungal
activities and could be alternatives for chemical pesticides [Gabaston et al., 2017; Pezet et al.,
2003]. These studies indicate also that the most active compounds are the oligomeric stilbenes
like tetramers [Schnee et al., 2013]. However, most of the grapevine extracts contain mainly
(E)-resveratrol (monomer) and (E)--viniferin (dimer), that exhibit a moderate effect against
fungal pathogens. Besides, the sourcing of these extracts is limited by raw material availability.
In order to overcome these limitations, we proceeded to a hemisynthesis of stilbene derivatives
from resveratrol by oxidative coupling using metals to produce oligomeric stilbenes. Produced
compounds were identified by MS and NMR spectrometry. The whole reaction product extract
and the separated pure derivatives antifungal activity was then tested in vitro against Plas-
mopara viticola and Botrytis cinerea. Results showed a high activity of the reaction extract
against both pathogens, with CI50 highly inferior to those of resveratrol and grapevine cane
extracts.
Keywords: grapevine, stilbenes, hemisynthesis, antifungal activity
∗
Speaker
†
25
Plant metabolomics: A tool for
authentication of medicinal plant products
∗† 1
Sarada Tetali , Pulok K. Mukherjee 2 , Agepati S Raghavendra 3
1
Prof. Sarada D. Tetali – Department of Plant Sciences, School of Life Sciences, Prof. CR Road,
Gachibowli, Hyderabad 500046, Telangana State, India
2
Prof. Pulok K. Mukherjee – Dept. of Pharmaceutical Technology, School of Natural Product Studies,
Jadavpur University, Kolkata – 700032, India
3
Prof. A.S. Raghavendra – Department of Plant Sciences, School of Life Sciences, Prof. CR Road,
The current global herbal medicine market size is estimated to cross USD 50 billion and
is expected to exhibit enormous growth over the forecast period. This can be attributed to
the increase in the interest of consumers towards traditional medicines (Ayurveda, Unani and
Traditional Chinese and European Medicine) because of their health benefits. In parallel, multi-
target therapy is been appreciated globally, even by conventional medical practitioners and
pharmacologists as one drug – one target is no longer satisfied with the treatment of several
multifactorial diseases like cardiovascular, neurological disorders, diabetes, cancer. New con-
cepts of poly- / network pharmacology are penetrating into drug discovery programs, and are in
line with market demand for herbal medicine. This is setting a trend, in developed and devel-
oping countries, towards the integration of complementary and alternative medicine therapies
with conventional medical practice. Medicinal plant products are complex chemical mixtures
unlike the drugs used in conventional medicine, therefore authentication of medicinal plants and
their products has become hypercritical. Chemical/metabolomic profiling of medicinal plants is
one of the superior methods which can be achieved by employing nuclear magnetic resonance
(NMR) or mass-spectrometry (MS). Hyphenated mass spectrometric technics with liquid or gas
chromatographic separation of plant products are the method of choice as they are relatively
higher sensitive and reliable techniques. However, it is ideal to use a combination of several
analytical platforms like NMR, LC-MS/MS, GC-MS/MS, CE/MS, HPTLC-MS/MS for com-
plete profiling of natural products. In our laboratory, extracts of some of the medicinal plants
selected for evaluating their pharmacological activities are subjected to LC-MS and GC-MS.
The metabolomic data of medicinal plants obtained will be presented highlighting on the iden-
tification of species-specific metabolites.
Keywords: medicinal plants, Metabolomics, traditional medicine, LCMS, GCMS
∗
Speaker
†
26
Exploring metabolome in extremophile
plants from Atacama desert
Thomas Dussarrat ∗† 1,2 , Amélie Flandin 2,3 , Cédric Cassan 2,3 , Stéphane
Bernillon 2,3 , Sylvain Prigent 2,3 , Yves Gibon 2,3 , Rodrigo A. Gutiérrez 1 ,
Dominique Rolin 2,3 , Pierre Pétriacq‡ 2,3
1
Pontificia Universidad Católica de Chile Plant Systems Biology Lab – Casilla 306, Santiago 22, Chile
2
Biologie du fruit et pathologie – Institut National de la Recherche Agronomique : UMR1332 – Centre
INRA Bordeaux-Aquitaine 71 avenue Bourlaux BP81 F-33883 Villenave dÓrnon, France
3
Plateforme MetaboHUB-Bordeaux, MetaboHUB, PHENOME-EMPHASIS – Institut national de la
recherche agronomique (INRA) – 71 av E. Bourlaux, 33140 Villenave d’Ornon, France
Abiotic stresses are major limitations for agricultural productivity worldwide. The study of
plant species that survive and thrive under extreme conditions offers a unique opportunity to im-
prove our understanding of adaptation to hostile ecosystems and therefore provide novel insights
into the mechanisms of plant tolerance to harsh climates1. In an ongoing program, Gutiérrez’s
laboratory performed a transcriptomic study on 32 Atacama plant species and highlighted three
major clusters underlying adaptation to marginal lands. Here, we propose to conduct com-
parative metabolomic profiling to explore and understand the metabolic features of selected
extremophile plants from Atacama desert in their hostile natural environment. Quantitative
targeted metabolomics of primary metabolites have been obtained by biochemical phenotyping
to provide information on metabolic markers of physiological functions. Statistical analyses
showed significant differences between plant species and environmental conditions suggesting
a key genotype-environment interaction. Next, high-resolution mass spectrometry was used
to characterize metabolic profiles from selected Atacama extremophiles and to determine their
alterations induced by both genotype and environment. Several discriminant metabolomics pat-
terns were unravelled for further putative annotation using chemical databases. Thereafter,
metabolic signatures of the Atacama plant species will be integrated in metabolic networks.
Finally, comparisons of metabolic profiles and stoichiometric networks between Atacama ex-
tremophiles and both model and agronomic species will be performed. Altogether, our prelim-
inary results demonstrate that metabolomics on extremophiles provides relevant milestones for
the discovery of molecules of interest for both plant tolerance and human health.
1. Dı́az, F.P., Latorre, C., Carrasco-Puga, G., Wood, J.R., Wilmshurst, J.M., Soto, D.C.,
Cole, T.L., and Gutiérrez, R.A. (2019). Multiscale climate change impacts on plant diversity
in the Atacama Desert. Global Change Biology 25, 1733–1745.
Keywords: Abiotic stress, Extremophile plants, Metabolomics, HRMS, biochemical phenotyping
∗
Speaker
†
‡
27
New Bioactives & HCS approaches
combined with Metabolomics
∗ 1
Constanze Mueller
1
Helmholtz Zentrum München – Ingolstaedter Landstraße 1 85764 Neuherberg, Germany
Traditionally, primary screenings for bioactivity mainly include single-target biochemical as-
says. In recent years, phenotypic screenings using a multiplexed image-based technology (High-
Content-Screening, HCS), have emerged for such primary screenings to deliver more comprehen-
sive data. HCS provides information on multiple levels of cell physiology, which is summarized in
a cytological profile consisting out of hundreds of cellular features (1). We combine this promis-
ing technology with non-targeted ultra-high resolution metabolomics of natural extracts for fast
discovery of novel bioactives. Furthermore, we include the metabolomics analysis of the treated
cells, which allows the assessment of functional aspects (functional metabolomics). Using the
combined data, we can extract relevant molecular signatures and predict their putative mode-
of-action by comparing the cytological profiles to collections of molecules with known effects,
or discover novel compound activities. This analysis pipeline allows a rationally design and
focus on the most promising candidates in the following targeted fractionation and extraction
of bioactive molecules. The further combination with data-depended UPLC-ToF-MS2 analysis
allow a fast recognition of putative rediscoveries (meaning structurally new but related molecules
to known bioactives). Importantly, our approach is suitable for both single molecules as well as
complex biological extracts (2,3) .
1 Kremb, S. & Voolstra, C. R. High-resolution phenotypic profiling of natural products-induced
effects on the single-cell level. Scientific reports 7, 44472, doi:10.1038/srep44472 (2017).
2 Kremb, S., Muller, C., Schmitt-Kopplin, P. & Voolstra, C. R. Bioactive Potential of Marine
Macroalgae from the Central Red Sea (Saudi Arabia) Assessed by High-Throughput Imaging-
Based Phenotypic Profiling. Marine drugs 15, doi:10.3390/md15030080 (2017).
3 Muller, C., Kremb, S., Gonsior, M., Brack-Werner, R., Voolstra, C. R., Schmitt-Kopplin, Ph.
Advanced identification of global bioactivity hotspots via screening of the metabolic fingerprint
of entire ecosystems. Scientific reports, under review
Keywords: (functional) metabolomics, natural products, bioactives, high content screening, pheno-
typing
∗
Speaker
28
Metabolomics to exploit the primed immune
system of tomato fruit
Estrella Luna 1 , Amélie Flandin 2 , Cédric Cassan 3 , Chloé Chevanne 4 ,

Camélia Feyrouse Kadiri 5 , Yves Gibon 3,6 , Pierre Pétriacq ∗† 7,8
1
University of Birgmingham – School of Biosciences, Univ. Birmingham, Birmingham (UK), United
Kingdom
2
INRA Bordeaux Nouvelle Aquitaine - MetaboHUB-Bordeaux – INRA Bordeaux Nouvelle Aquitaine –
UMR 1332 Biologie du Fruit et Pathologie, INRA, Univ. Bordeaux, 71 av E. Bourlaux, 33140 Villenave
d’Ornon (FR) Plateforme Métabolome Bordeaux, MetaboHUB-PHENOME, Centre INRA de
Nouvelle-Aquitaine Bordeaux, 71 av E. Bourlaux, 33140 Villenave d’Ornon (FR), France
3
INRA BOrdeaux Nouvelle Aquitaine - MetaboHUB-Bordeaux – CGFB, INRA, Univ. Bordeaux,
UMR1332 Biologie du Fruit et Pathologie – UMR 1332 Biologie du Fruit et Pathologie, INRA, Univ.
Bordeaux, 71 av E. Bourlaux, 33140 Villenave d’Ornon (FR) Plateforme Métabolome Bordeaux,
MetaboHUB-PHENOME, Centre INRA de Nouvelle-Aquitaine Bordeaux, 71 av E. Bourlaux, 33140
Villenave d’Ornon (FR), France
4
Université de Bordeaux – Université de Bordeaux – Sciences et technologies 351 cours de la Libération
CS 10004 33 405 Talence CEDEX, France
5
Université de Bordeaux – Université de Bordeaux (Bordeaux, France) – Sciences et technologies 351
cours de la Libération CS 10004 33 405 Talence CEDEX, France
6
Biologie du fruit et pathologie (BFP) – Université Sciences et Technologies - Bordeaux I, Université
Victor Segalen - Bordeaux II, Institut national de la recherche agronomique (INRA) : UMR1332 –
Centre INRA Bordeaux-Aquitaine 71 avenue Bourlaux BP81 F-33883 Villenave d’Ornon, France
7
Plateforme MetaboHUB-Bordeaux, MetaboHUB, PHENOME-EMPHASIS – Institut national de la
recherche agronomique (INRA) – 71 av E. Bourlaux, 33140 Villenave d’Ornon, France
8
Biologie du fruit et pathologie – Institut National de la Recherche Agronomique : UMR1332 – Centre
INRA Bordeaux-Aquitaine 71 avenue Bourlaux BP81 F-33883 Villenave dÓrnon, France
Tomato is a major crop suffering substantial yield losses from diseases as fruit decay at a
postharvest level can claim up to 50% of the total production worldwide1. Due to the envi-
ronmental risks of fungicides, there is an increasing interest to exploit plant immunity through
priming, which is an adaptive strategy that improves plant defensive capacity by stimulat-
ing induced mechanisms2. Broad-spectrum defence priming can be induced by the compound
ß-aminobutyric acid (BABA)3. In tomato plants, BABA induces resistance against various
fungal and bacterial pathogens and different methods of application result in durable protec-
tion4,5. Here, we examined whether treatment of tomato plants with BABA resulted in a
durable induced resistance in tomato fruit against Botrytis cinerea, Phytophthora infestans and
Pseudomonas syringae. Targeted and untargeted metabolomics were used to investigate the
metabolic regulations that underpin priming of tomato fruit against pathogenic microbes that
present different infection strategies. Assessment of infection showed a statistically significant
reduction of disease symptoms in tomato fruit that originated from BABA-primed plants, thus
confirming the broad effectiveness of BABA priming against various pathogens. Metabolomic
analyses revealed major changes after BABA treatment and after infection. Remarkably, primed
responses depended entirely on the type of infection, rather than showing a common fingerprint
of BABA-induced priming. Altogether, our results demonstrate that metabolomics is particu-
larly insightful towards a better understanding of defence priming in fruit. Further experiments
∗
Speaker
†
29
are underway in order to identify key metabolites that mediate broad-spectrum BABA-induced
priming in tomato fruit.
Keywords: metabolomics, tomato, priming, BABA, Botrytis cinerea
30
MetaboHUB-Bordeaux Facility
Stéphane Bernillon 1 , Cécile Cabasson 1 , Cédric Cassan 1 , Grégory Da

Costa 1 , Catherine Deborde 1 , Amélie Flandin 1 , Laetitia Fouillen 1 , Yves
Gibon 1 , Daniel Jacob 1 , Annick Moing 1 , Sébastien Mongrand 1 , Eric
Pédrot 1 , Pierre Pétriacq ∗† 1 , Sylvain Prigent 1 , Elodie Renouf 1 , Tristan
Richard 1 , Dominique Rolin 1 , Caroline Rouger 1 , Josep Valls 1 , Pierre
Van Delft 1
1
MetaboHUB-Bordeaux Facility aims to meet the metabolomics and lipidomics needs related
to the research activities of academic and private laboratories. Focusing on metabolites mostly
from plant origin, it carries out adaptations and technological developments, designs and im-
plements analytical strategies and bioinformatic tools for the study of metabolome, lipidome,
metabolic phenotyping (robotic measurements of metabolites and enzymatic activities) and
fluxome. It represents a major plant metabolomics site in France and works in close collab-
oration with other European metabolomics platforms. Three French research institutes support
MetaboHUB-Bordeaux Facility (University of Bordeaux, INRA and CNRS) via three laborato-
ries: UMR1332 Fruit Biology and Pathology INRA/Bordeaux University, UMR5200 Membrane
Biogenesis Laboratory CNRS/Bordeaux University, and the Oenology Research Unit - EA4577 -
USC1366 INRA. The Facility is also associated with two transfer cells (Polyphenols Biotech and
LEB Aquitaine Transfert) which ensure the relationship with industry at regional and national
level to meet the technological and scientific needs of the company in connection with the fields
of lipidomics and analysis of metabolites of interest such as polyphenols. Its members include
researchers, research professors and engineers, and technical staff from the University and re-
search institutes. This crosscutting of human resources, skills, networks and biological analysis
activities promotes the Facility catalytic role in identifying and promoting synergies between
research fields, scientific innovation and the transfer of knowledge to society not only through
education but also through technology transfer and service delivery to meet industrial and so-
cietal needs. MetaboHUB-Bordeaux Facility is one of the four partners of INBS MetaboHUB
(2013-2024), which it coordinates. It also participates in the INBS PHENOME-EMPHASIS
(2013-2024) and its European version, as well as the transnational network EPPN2020.
Keywords: metabolomics, biochemical phenotyping, lipidomics, plants, high, throughput, chemo-

metrics, enzyme activities, MetaboHUB, PHENOME, EMPHASIS, polyphenols, mass spectrometry,
NMR
∗
Speaker
†
31
Therapeutic biomolecules from plants with
anticancer properties
∗ 1
Irfan Ahmad Ghazi
1
University of Hyderabad – University of Hyderabad, Gachibowli, Prof CR Rao Road,
Hyderabad-500046., India
Plants have been used as therapeutic agents since ancient times as it possesses significant
source of health promoting biomolecules that have ailment curing properties. Several researches
were made to explore the identification of phytochemicals from plants with anticancer properties
at global level. These phytochemicals neutralize free radical damages, contribute to changes in
DNA methylation pattern, induce apoptosis through the generation of intracellular reactive
oxygen species, suppress different inflammatory cytokines including interleukins (IL) -1,-2,-6,-8,
as well as tumor necrosis factor-α (TNF- α) etc. Phytochemicals which have strong anti-oxidant
properties are generally believed to inhibit chronic diseases including cancer by neutralizing
free radical mediated injury and affecting different epigenetic molecular mechanisms. These
phytochemical includes Withaferin A, Genistein, Isoferulic acids, Luteolin, Coumarins, Apigenin,
Curcumin etc. which is known to inhibit different types of cancer cells. These phytochemicals
acts on cancer cells and mediate both intrinsic and extrinsic apoptosis and induce the expression
of different genes such as p53, Bax etc and mediate cell cycle arrest in G1 phase and finally
help in cancer chemotherapy. Curcumin from Curcuma longa a well known biomolecule acts
as an anti-cancer compound by targeting genome and upregulates the expression of different
tumor growth suppressors. Several well-established anticancer drugs and compounds have been
reported from plants such as vinblastine & vincristine from Catharanthus roseus, Paclitaxel from
Taxus brevifolia, Camptothecine from Camptotheca acuminata, Panduratin A from Kaempferia
pandurata etc have significantly influenced the chemotherapy of different types of cancer. Many
plant based compounds are classified under polyphenols (flavonoids) which are known to exhibit
different therapeutic activities including anticancer properties against different types of cancers.
Several medicinal plants need to be explored to identify more potent therapeutic compound with
anticancer properties.
Keywords: Phytochemicals, Anticancer, Polyphenols, Apoptosis, Chemotherapy.
∗
Speaker
32
Plant-based food supplement formulation in
Europe
∗ 1
Sophie Lafay
1
GYNOV SAS – GYNOV SAS – 2 RUE DE LA GARE 91630 MAROLLES EN HUREPOIX, France
Before the 2000s, the formulation of food supplements was not regulated in Europe, or at
best depended on conventional foods laws. This regulatory vagueness gave the manufacturer
great freedom both in terms of product formulation and health claims. The regulatory definition
of food supplements in Europe appeared in 2002 (Directive 2002/46/CE) and, since then, the
associated regulations/amendments have continuously grown. Rules adapted to this particular
food are in place to ensure the safety and non-deception of the consumer.
Plants, herbal preparations, extracts are among the most commonly used ingredients in the
field of food supplements. Vitamins and minerals are also frequent. However, the originality of
innovative formulas is most often based on a vegetable / botanical ingredient - and probiotics
for about 5 years.
Therefore, along with the rules on food supplements, a new regulation related to plants and
plant extracts has emerged. In 2009, a working group of the European Food Agency (EFSA)
has worked on a compendium to establish a list of plants and botanical preparations used in the
preparation of food supplements and their risk of use (EFSA Journal 2009; 7(9):281). It was
followed by the publication of a guidance by the EFSA Scientific Committee (EFSA Journal
2009; 7(9):1249). Specific guidelines have also been published for good practices in the selection
and the manufacture of food supplements (Quality of Botanical Preparations, Food Supplement
Europe (FSE), October 2014; Quality Guide for Botanical Food Supplements, European Botan-
ical Forum (EBF), July 2011).
The purpose of this presentation is to introduce European and French rules for formulating
herbal food supplements, to explain how those imposed rules are in fact guides to guarantee
maximum consumer safety and to set up quality procedures specific to this type of finished
product and to ensure quality production.
Keywords: food supplement, plants, botanical preparation, regulation, quality
∗
Speaker
33
The mechanism of plants & drug
interactions
∗ 1
François Petitet
1
Herbéo – Herbéo – 31 Rue des Ayres, 33000 Bordeaux, France
Many people treated with pharmaceutical drugs use complementary herbal treatments. The
concomitant administration of a large set of molecules can induce different interactions either at
the level of their mechanisms of action or at the level of their metabolism. Pharmacodynamic
interactions occurs at the level of mechanisms of action while pharmacokinetic interactions con-
cern absorption, metabolism or elimination of active ingredients.
Herb-drug interactions is particularly complex to predict due to the diversity and the molecular
variability of plant extracts and the low clinical predictability of available in vitro data. More-
over, most often, very few data on the molecular mechanisms of action and the pharmacokinetics
of each individual component of the plant extract are available.
The most relevant observations will ultimately come from clinical experience demonstrating
the existence or absence of interactions. We will discuss several typical cases of herbal-drug
interactions with commonly used medicinal plants.
Keywords: Herb, Drug Interactions, Pharmacokinetic, Clinical studies
∗
Speaker
34
Age induced Circadian Rhythm dysfunction
in Central and Peripheral Clocks :
Therapeutic Interventions using Herbal
Antioxidants
∗ 1
Anita Jagota
1
Anita Jagota – Neurobiology and Molecular Chronobiology Laboratory, Department of Animal
Biology, School of Life Sciences, University of Hyderabad, Hyderabad-500 046, India., India
Aging is associated with changes in several basic parameters of circadian timing system
(CTS) in mammals leading to circadian dysfunction. The suprachiasmatic nucleus (SCN) in hy-
pothalamus contains a light-entrained circadian clock. It is involved in regulation of neuronal,
endocrine and behavioral rhythms through the expression of various clock genes. It regulates
the rhythmic production and release of melatonin (messenger of darkness) from pineal gland
involving close interaction of core circadian machinery with a network of interconnected tran-
scriptional and translational feedback loops.
To understand the age induced stoichiometric alterations in interactomes of daily chronomics in
neurodegenerative changes in the functional integrity of CTS, daily rhythms in various parame-
ters in SCN at variable time points (Zeitgeber time (ZT) - 0, 6, 12 and 18) in three age groups (3
(adult), 12 and 24 months) of male Wistar rats maintained in light–dark conditions (LD 12:12).
We report here, the age-induced change in interactions between various 5-HT metabolism com-
ponents by middle age (12 m) changing further by 24 m. The m-RNA expression for clock genes
such as bmal1, per1, per2, cry1, and cry2 was rhythmic in SCN of adult rats. However in 12
and 24 m, the phases of expression of these genes were significantly altered with abolition of
daily rhythms of rCry1, rCry2 and rBmal1 in 24m. Differential alterations with aging in the
levels and chronomics of 2-D protein profiles and locomotor rhythms were observed.
Therapeutic differential restoratory effects of administration of various herbal antioxidants espe-

cially Curcumin from Curcuma longa (turmeric) and withanolides and alkaloids from Withania
somnifera (Ashwagandha) on aging and neurodegeneration were studied on age induced Circa-
dian dysfunction and desynchronization in various molecular parameters.
This work may prove useful towards targeting novel treatments for circadian dysfunction, good
health and longevity.
Keywords: Aging, circadian rhythms, curcumin, Ashwagandha, antioxidants
∗
Speaker
35
Metabolite based evaluation and quality
control of spices
∗† 1
Agepati Raghavendra , Pulok K. Mukherjee 2 , Sarada Tetali‡ 3
1
School of Life Sciences, University of Hyderabad – Hyderabad 500046, Telangana,, India
2
Prof. Pulok K. Mukherjee – Dept. of Pharmaceutical Technology, School of Natural Product Studies,
Jadavpur University, Kolkata – 700032, India
3
Prof. Sarada D. Tetali – Department of Plant Sciences, School of Life Sciences, Prof. CR Road,
Spices are important value additions for human food, being rich sources of secondary metabo-
lites used for culinary purpose as well as for medicinal value. Metabolomics is a modern approach
for comprehensive analysis of both primary and secondary metabolites. Despite the potential
use, metabolite data of Indian medicinal plants and spices are extremely limited. My talk empha-
sizes the importance of metabolomics of Indian spices while pointing out the use of metabolomics
data for quality evaluation and scientific validation of commercial products, besides formulations.
There are various factors affect the quality and standards of food and herbal products, including
their cultivation, collection, storage and post-harvest processing. Plant metabolomics provides a
comprehensive understanding of the spectrum of phytochemical constituents of plants, at every
stage of field to lab and then to market. Advances in mass spectrometry (MS) based platforms
like GC-MS and LC-MS, helped in the improvement, perfection and the speed of metabolomics.
As a result, the technique of metabolomics is fast becoming a versatile tool for optimal use of
known spices/medicinal plants, besides the discovery and development of new ones. Our lab
is involved in extensive studies on secondary metabolites of Curcuma longa and Curcuma aro-
matica. Based on metabolomics data, we can identify varieties that are rich in curcuminoids,
and even evaluate the quality of commercial samples. Similar approaches have been used with
other spices, such as ginger, vanilla, pepper, onion and garlic. While presenting case-studies of
some of the spices, emphasis would be made of targeted use of metabolomics for quality assess-
ment, optimization of extraction/processing, evaluation of varieties and identifying the optimal
environmental conditions, for improving the commercial value of spices.
Keywords: Ginger, Herbs, Medicinal Plants, Metabolomics, Phytochemicals, Spices, Turmeric
∗
Speaker
†
‡
36
The French Nutrivigilance scheme
∗ 1
Aymeric Dopter
1
Agence nationale de sécurité sanitaire de lálimentation, de lénvironnement et du travail – ANSES -
Agence nationale de sécurité sanitaire de lálimentation, de lénvironnement et du travail-0 – 14 rue
Pierre et Marie Curie94701 MAISONS-ALFORT Cedex, France
The French agency for food, environmental and occupational health and safety (Anses) has
developed a vigilance scheme for registering and analysing the side effects of food supplements,
novel foods, fortified foods and foodstuffs intended for particular nutritional uses. This spe-
cific vigilance, called ”Nutrivigilance”, was created in 2009 on a regulatory basis. Since then,
it collected more than 4,500 declarations of side effects, which involved predominantly food
supplements. The Nutrivigilance thus enables Anses to trigger scientific expertise concerning
different ingredients, including plants. Through few examples of plants, such as Dioscorea spp.,
Tagetes erecta and Citrus aurantium a short presentation of the Nutrivigilance porcess is given
for illustrating the way from declaration of side effects to the publication of scientific opinions
in the area of plant safety.
Keywords: Nutrivigilance, food supplement, plant safety
∗
Speaker
37
Analysis of plant extracts quality in
industrial settings
∗† 1
Lucile Berthomier , Valérie Bardot 2 , Anne Leblanc 3 , Michel
Dubourdeaux 2
1
Pileje Industrie – Pileje industrie – Naturopole Z.I. Les Tiolans 03800 Saint-Bonnet-de-Rochefort,
France
2
Groupe Pileje – Pileje – 37 Quai de Grenelle 75015 Paris, France
3
Pileje Laboratoire – Pileje – 37 Quai de Grenelle 75015 Paris, France
PiLeJe promotes the health of each individual. Our aim is to offer high quality health solu-
tions to steer everybody towards their optimal physiological state, maintain that state for as long
as possible and restore it when necessary. Botanical innovation, micronutrition, probiotic exper-
tise and industrial excellence are at the heart of our services. Part of our research and industrial
expertise is focused on the production of plant extracts with a phytochemical profile similar to
that of the plant totum, which is defined as all the active compounds present in the part of the
plant used for extraction, and on which the fundamental principles of additivity and synergy
are based. One of our objectives is to control the phytochemical composition of the extracts
we produce and to provide evidence of their biological activity. Fingerprint controls ensure the
reproducibility and standardization of the final extracts and can highlight biological properties
such as antioxidant activity. For instance, we determined the phytochemical profile of a fresh
Curcuma Longa rhizomes extract, produced according to the patented process Phytostandard R
(standardized extract marketed under the name EPS Curcuma, PiLeJe Laboratoire, France)
by combining different methods: HPTLC, HPLC, LC-MS, LC-MS/MS and NMR. We showed
that its profile was similar to that of the native plant and identified compounds from different
chemical families. Altogether the analyses showed that our extraction process allows to obtain
an extract of fresh Curcuma Longa rhizomes with a composition close to that of the plant totum.
We also demonstrated that this specific extract had anti-oxidative and analgesic effects.
Keywords: Turmeric, Phytochemical profil, HPTLC, LC, MS, NMR, Biological activities
∗
Speaker
†
38
DNA-based approaches for the botanical
authentication of medicinal plants and plant
food supplements
∗† 1,2
Joana Amaral
1
CIMO, Instituto Politécnico de Bragança – Bragança, Portugal
2
REQUIMTE-LAQV, Faculdade de Farmácia, Universidade do Porto – Porto, Portugal
Medicinal plants have been used over the centuries for keeping or restoring health. Presently,
besides their implemented use in traditional medicine and in traditional herbal medicinal prod-
ucts (THMP), they are also largely used as ingredients of plant food supplements (PFS). PFS
are legally considered as foods, both in the European Union and in the United States of America,
and have been showing a fast growth in consumption and popularity over the last decade. With
the increase of the global market and the growing demand for PFS, these products, which are
generally sold at high market prices, are prone to be adulterated for economic gains. Botanical
adulteration is among the safety issues and frauds reported to occur in PFS. This includes the
intentional substitution of high-priced medicinal plants by other similar, the addition of fillers
to increase product bulk (normally cheap plants) or the swap of plants due to misidentification
of species. Misidentification can occur mainly with wild collected plants, being also potentiated
by trade globalization phenomena that prompt the increasing use of plant species from foreign
regions in the EU. Therefore, to ensure the safety and quality of PFS, accurate identification of
plant species along the production chain, from raw materials until the finished products, is of
utmost importance to protect the health and expectations of consumers. In this sense, DNA-
based techniques have played a crucial role by allowing the development of a wide range of tools
available for the identification of medicinal plant species. The use of DNA-based techniques and
their recent advances will be presented, and the advantages and drawbacks of their application
to authenticate medicinal plants and PFS will be discussed. Particularly, in the case of PFS,
which frequently present complex formulations, the identification of plant material used in their
production is often a research challenge.
Keywords: adulteration, botanicals, plant food supplements
∗
Speaker
†
39
1H NMR versus UHPLC-DAD-MS chemical
profiling of red yeast rice dietary
supplements and quantification of
monacolins
Rabab Hachem 1 , Gaëtan Assemat 1 , Stéphane Balayssac 1 , Nathalie

Martins-Froment 2 , Véronique Gilard 1 , Robert Martino 1 , Myriam
Malet-Martino ∗ 1
1
Laboratoire SPCMIB, Université Paul Sabatier – Université Paul Sabatier (UPS) - Toulouse III – 118
route de Narbonne, 31062 TOULOUSE cedex 9, France
2
Institut de Chimie de Toulouse – Université Toulouse III - Paul Sabatier – 118 Route de Narbonne
31062 Toulouse cédex 9, France
Red yeast rice dietary supplements (RYR DS) are largely sold in Western countries for their
cholesterol-lowering effect due to monacolins, mainly monacolin K (MK) which is lovastatin, the
first statin drug on the market.
1H NMR was used as a simple, direct and non-selective method to establish quickly the chemical
profiles of 31 RYR DS and to quantify their monacolin contents, which were compared to those
obtained by UHPLC-DAD-MS, the gold standard technique for a detailed determination of all
the monacolins existing in RYR DS.
Among all the 1H resonances of the monacolins found in RYR, only those of the ethylenic
protons of the hexahydronaphtalenic ring at 5.84 and 5.56 ppm are suitable for quantification
because they show no overlap with the matrix signals. The content in monacolins could be
determined in 28 DS over the 31 analyzed as for 3 DS it was below the limit of quantification
of the method (≈0.25 mg per capsule or tablet). The content in total monacolins per unit dose
determined by 1H NMR was close to that measured by UHPLC as shown by the good linear
correlation between the 2 sets of values (slope 1.00, y-intercept 0.113, r2 0.986).
Thirteen of the 31 RYR DS analyzed (i.e. 42%) did not provide label information on the
concentration of monacolins and only 9 of the 18 formulations with an indication (i.e. 50%)
contained the declared amount of monacolins.
In conclusion, health authorities should impose to manufacturers a strict control of the quality
of the RYR DS and a label clearly indicating the amounts of MK in its two forms and total
monacolins per serving. The knowledge of these quantities would be of great interest to assess
the ability of the marketed RYR products in reducing cholesterol level.
Keywords: Red yeast rice, 1H NMR, UHPLC, DAD, MS, Mass spectrometry, Monacolins
∗
Speaker
40
Evaluation of low-field proton NMR
spectroscopy for quality control of cinnamon
samples
Nao Wu 1 , Stéphane Balayssac 1 , Saı̈da Danoun 1 , Myriam Malet-Martino

∗ 1
, Véronique Gilard† 1
1
This study aimed to evaluate the ability of proton low-field NMR (Nuclear Magnetic Reso-
nance) as a method for quality control of cinnamon samples.
Cinnamon is a well-known spice with many uses for food, health products or cosmetics. Two
varieties of Cinnamon are commonly available, true cinnamon or Ceylon cinnamon which cor-
responds to the Cinnamomum verum species and Chinese cassia or Chinese cinnamon which
has several origins and corresponds to the Cinnamomum cassia species. True cinnamon has a
characteristic taste different from Cassia one; it is slightly sweeter and not as strong because of
different chemical composition. Ceylon cinnamon has low levels of coumarin by comparison to
all other varieties of cinnamon. Coumarin is known to be hepatotoxic and a maximum tolerable
daily intake of 0.1 mg/kg has been defined by the EFSA to ensure safety. As Cinnamomum
cassia is cheaper, it is more and more widely available in Europe; it is thus relevant to propose
new quality control methods for the analysis of cinnamon samples.
Twenty-eight samples were analyzed, fourteen were marketed for cooking and fourteen as di-
etary supplements. First, high-field NMR analysis was used to identify active compounds from
cinnamon samples. (E)-cinnamaldehyde was the main compound detected along with minor sig-
nals of coumarin, (E)-cinnamic acid, eugenol, methoxy-cinnamaldehyde, and cinnamyl alcohol.
Quantification of active compounds was performed using an internal reference. Then, low-field
NMR experiments were carried out on all samples. Characteristic signals of (E)-cinnamaldehyde
and coumarin when present in the samples were detected. Although absolute quantification can-
not be reached using low-field NMR due to experimental limitations, the statistical treatment
of low-field NMR data has proved to be an interesting way of classifying samples and pseudo-
quantitating their contents. For dietary supplements, this approach can identify samples posing
a risk to the health of consumers.
Keywords: Cinnamon, NMR, compact NMR, flavor, dietary supplement, nutraceuticals
∗
Speaker
†
41
High-field and low-field NMR for unveiling
adulteration of dietary supplements by
pharmaceutical compounds or other actives
Gaëtan Assemat 1 , Stéphane Balayssac 1 , Rabab Hachem 1 , Guilhem

∗
Pagès 1 , Robert Martino 1 , Véronique Gilard 1 , Myriam Malet-Martino
1
1
Nuclear magnetic resonance (NMR) spectroscopy is a unique tool for detection, structural
characterization, and quantification of compounds in complex mixtures. In this study, proton
NMR was applied to the quality control of dietary supplements (DS) to investigate whether
active compounds were fraudulently added to DS by unscrupulous manufacturers to improve
their effects. Adulteration of ”100% natural” sexual enhancement and weight-loss dietary sup-
plements was studied.
The study was conducted on a 500 MHz NMR spectrometer demonstrating the power of conven-
tional NMR for the screening of these complex mixtures and the quantification of actives. Due
to cost constraints, NMR is rarely used in routine quality control analysis but the recent release
of benchtop cryogen-free low-field NMR spectrometers represents a technological break in the
NMR field. Thus, we also evaluated the potential of a recent benchtop cryogen-free 60 MHz
spectrometer for detection of adulteration and we demonstrated that after very simple and rapid
sample preparation, around 20 min of NMR recording are sufficient to detect the adulterants.
We also showed that the quantification by the internal standard method can be done on the
low-field NMR spectrometer and leads to results similar to those obtained with high-field NMR.
Among the 301 DS marketed for erectile dysfunction and 180 slimming products analyzed,
62% and 51% respectively were tainted. Major adulterants detected were phosphodiesterase-5
inhibitors (sildenafil, tadalafil, vardenafil) and analogs for the first class, whereas sibutramine
and phenolphthalein were the major adulterants in slimming DS.
This study demonstrated that NMR and particularly benchtop low-field NMR, considering the
cost and space efficiency of these compact spectrometers, could be introduced in quality control
laboratory for routine analysis as an initial routine screening tool for unraveling adulteration.
Keywords: High, field NMR, low, field NMR, dietary supplements, erectile, weight, loss
∗
Speaker
42
Polyphenols Biotech transfer unit
∗ 1
Elodie Renouf
1
Polyphenols Biotech- ADERA – Polyphenols Biotech- ADERA – ISVV 210 Chemin de Leysotte / CS
50008 33882 Villenave d’Ornon Cedex, France
Since 2004, Polyphenols Biotech has served as one of four ISVV’s technology transfer units.
It specializes in phytochemistry (extraction, purification, analysis), in human health (biological
properties of plant extracts, bioavailability), plant health (evaluation of the effectiveness of nat-
ural products against diseases of the vine) and plant cell culture.
A team of highly qualified and experienced research scientists provides expertise for companies
in the pharmaceutical, cosmetics, agri-food, nutraceuticals, phytosanitary sectors. Providing an
interface between academic research and industry, this transfer unit allows companies to:
- access state-of-the-art research
- make use of our specialized knowledge in the chemistry and biology of polyphenols
- develop new uses for polyphenols (product development)

Polyphenols Biotech, approved CIR, offers service provisions or R&D contracts to meet the
needs of its customers under strict confidentiality.
Keywords: polyphenols, natural substances, extraction, analysis, purification, human health, plant
health, plant cell culture
∗
Speaker
43
Detection and quantification of stimulants
in supplements for athletes by 1H NMR
Stéphane Balayssac ∗ 1 , Gaëtan Assemat 1 , Véronique Gilard 1 , Marzena

Wójtowicz-Zawadka 2 , Myriam Malet-Martino† 1
1
2
Polish Anti-Doping Laboratory – Trylogii 2/16, 01-982 Warsaw, Poland, Poland
Dietary supplements are extremely popular and widely available in the world of sports. Ath-
letes, amateur or professional, frequently use supplements to enhance sporting abilities and their
consumption continues to increase every year. Substances banned in sport by the World Anti-
Doping Agency (WADA) such as e.g. anabolic agents, hormone and metabolic modulators, and
stimulants (https://www.wada-ama.org/sites/default/files/wada 2020 english prohibited list 0.pdf)
are present in supplements that can be purchased on the Internet.
Twenty-five supplements were bought on websites from Poland and France and analyzed by
high-field proton Nuclear Magnetic Resonance (1H NMR). This analytical method, highly robust
and reproducible, was employed to establish the profile of each supplement and to identify and
quantify the prohibited molecules potentially present. Thirteen (i.e. 52%) contained substances
banned by the WADA or known to be dangerous, although there were no anabolic steroids as
adulterants. The prohibited molecules were sometimes indicated on the label but not always.
Several stimulants were identified: phenethylamine (PEA) and its derivatives (N-methyl-PEA,
b-methyl-PEA, N,N-dimethyl-PEA, N-méthyl-b-methyl-PEA), dimethylbutylamine, dimethy-
lamylamine, and methylsynephrine. The beta-2 agonist higenamine was found in one formula-
tion and N-isopropyloctopamine, not prohibited by the WADA but known for its cardiac toxicity,
was detected in four supplements.
Although complex and expensive, 1H NMR can be viewed as a standard screening approach for
quality control of complex mixtures such as supplements for athletes. The authorities should
impose to manufacturers a strict control of the quality of the supplements they market.
Keywords: Athletes supplements, 1H NMR, WADA prohibited substances, stimulants
∗
Speaker
†
44
Quality control of active compounds
(icariins) in Epimedium grandiflorum herbal
supplements by HPLC-DAD and Mass
Spectrometry
∗ 1
Saı̈da Danoun , Ifunanya Okeke 1 , Stéphane Balayssac 1 , Véronique
Gilard 1 , Myriam Malet-Martino† 1
1
Epimedium grandiflorum has been used in traditional Chinese medicine for its anti-impotence
properties due to the presence of icariin, a relatively weak inhibitor of phosphodiesterase-5 in
comparison to substances like sildenafil. E. grandiflorum is also a common ingredient of herbal
remedies for sexual dysfunction. However, the prevalence of poor-quality supplements has made
determining the composition of active ingredients in marketed Epimedium herbal supplements
a shared interest between marketers and researchers alike. Due to the similar structures of the
prenylflavonoid glycosidic compounds found in E. grandiflorum, it was difficult to fully deter-
mine their identities and quantify their amounts within the herbal supplements based solely on
1D 1H NMR technique. We thus decided to use UPLC-HRMS and HPLC-DAD techniques for
these purposes. We observed a great disparity between the dietary supplements studied both in
terms of quantities and qualitative profiles of icariins.
Keywords: Epimedium, HPLC, DAD, mass spectrometry, icariins
∗
Speaker
†
45
Quantitative analysis of wine composition
applied to Bordeaux red wines by 1H-NMR
metabolomics
∗ 1
Ines Le Mao , Grégory Da Costa† 1 , Louis Gougeon 1 , François Guyon‡
2
, Tristan Richard§ 1
1
2
Service Commun des Laboratoires – Service Commun des Laboratoires (SCL), Bordeaux, France – 3
avenue du Dr. Albert Schweitzer, 33600 Pessac, France
Traceability of wine is today a consumer demand and a scientific challenge. The methods
of analysis must be able to control three fundamental parameters: the geographical origin, the
grape varieties, and the vintage. Quantitative Nuclear Magnetic Resonance (qNMR) may be
a great tool to help authenticate wines. The acquisition of a large number of wine parame-
ters requires a small volume and only a few minutes. This innovative analytical technique can
therefore be useful to characterize wines quality and authenticity particularly in the context of
priceless wine.
A NMR-based metabolomics method was developed to semiautomatically quantify many wine
components [1]. A comparative evaluation based on the similarity score (score s) of the results
obtained for three sets of high-value authentic wines and suspicious wines was studied by com-
paring the analyzes by traditional way of analysis for wine authentication and profiling by (ii)
g-NMR [2]. In order to estimate aging impact on wine composition, samples from the same
batch from old vintages were analysed. Results showed that q-NMR can detect cases of fraud
by comparison with the original wine provided by the estate, according to conclusions of official
methods.
A database of commercial French wines was built with q-NMR data to examine the specific
Bordeaux red wines fingerprinting [3]. Several statistical analyses were performed to classify
wines according to their geographical origin, vintage. Results revealed a singular imprint of
Bordeaux wines in comparison with other French wines, with classification rates ranging from
71% to 100%. Theses analysis highlighted several specific metabolites of Bordeaux red wines
and showed the influence of terroir in the discrimination. Also, Bordeaux subdivisions were
investigated, and effects of wines evolution during bottle aging and vintage were pointed out.
These studies provide a global and practical description of the potential of q-NMR for wine
authentication.
Keywords: 1H, NMR, wine classification, multivariate statistics, food control
∗
Speaker
†
‡
§
46
European and French regulations on claims
∗ 1
Ambroise Martin
1
Formerly member of the Efsa working group on claim (2006-2018) – 605C route du Bas Privas
F-69390 CHARLY, French Guiana
Assessment and management of nutrition and health claims is currently harmonized in the
European Union through the regulation 1924/2006, fully implemented since December 2012:
only the claims present in the EU Register of claims can be used on the labels and commercial
communication. Inscription in the Register requires an a priori positive assessment by the
European Food Safety Authority and an authorization by the European Commission through
the Standing committee on Plant, Animals, Food and Feed (PAFF). Efsa scientific assessment
considers 3 major issues, the characterization of the food and of the effect (beneficial for health),
and the strength of the scientific evidence through high-quality human study(ies). From more
than 4000 generic claims and more than 400 dossiers submitted to Efsa, only 261 claims out of
2337 entries examined have been authorized. In several trials against the European Commission,
the European Court of Justice never questioned the severity of the Efsa scientific judgment. On
the basis of these assessments and expecting a high level of rejection , the assessment of 1548
claims on botanicals has been put ”on hold” since 2010 by the EC and these claims can continue
to be used whereas new botanicals and new claims require the the full authorization process.
This situation limits the development of innovation in this sector. In addition, the setting of
nutrient profiles for foods bearing a claim expected since 2010 is also pending. In an effort for
solving these two issues, the REFIT process (Regulatory Fitness and Performance Programme)
has been applied, the results of which were expected in mid-2019 but are still expected. In the
present regulatory situation and waiting for possible new rules, EC and Efsa websites provide a
lot of helpful tools and guidances for preparing high-quality dossiers for the submission of new
products and/or new claims.
Keywords: Health claim, regulation, botanicals, scientific assessment
∗
Speaker
47
The Canadian Regulation - Natural Health
Products
∗ 1
Emilie Chauvigné
1
Valbiotis – Valbiotis – 12F rue Paul Vatine 17180 PERIGNY, France
Synadiet, and especially the working group on Natural Health Products (NHPs), is study-
ing the Canadian Regulation on Natural Health Products in order to propose a new European
Regulation based on the NHP model. This new product category would bring together the
existing categories : food supplements and herbal medicinal products. Created in 2003, the
Canadian model appears to have a more pragmatic approach than the existing European regu-
lations. Safety, quality and efficacy of each product are evaluated before the marketing. It seems
interesting to consider the acquired experience in Canada to update the European regulation.
Keywords: Natural Health Product / regulation
∗
Speaker
48
Food Supplements and Natural Health
Products: A new health approach as part of
a health prevention policy.
∗† 1
Christelle Chapteuil
1
Juvamine Laboratory – Juvamine Laboratory – 8 rue Christophe Colomb - 75008 Paris, France
Food supplements and Natural Health products are concentrated sources of nutrients, sub-
stances and plants, with nutritional or physiological effects, alone or in combination, marketed
in dose forms.
They are dedicated to healthy people who wants to maintain a good health balance.
The market is quite young, and has known a significant growth among the past 20 years. In
the same time, the regulatory framework has also considerably strengthened both at French and
European level.
How does the consumer understand the products? How does he use the products? What
are the main issues of this market?
The perception of health is changing: we are moving from a model where the individual is
passive and the system is driven by the supply to a model where the individual is active and
the system is driven by demand. The consumer wants to make his choice in term of health.
The key points to know about Food Supplements and Natural Health Products:
- Definition
- Regulatory framework
- Perception of consumer, trends, demand

- Health prevention and issues
Keywords: Food supplements, Natural Health Products, Market, Regulations, Health prevention
∗
Speaker
†
49
Impact analysis of healthcare cost savings
thanks to the use of targeted dietary
supplements in France
∗ 1 ∗ † 1
Ugo Feracci , Donatien Gamba
1
FrostSullivan – FrostSullivan – 24 Rue de Londres, France
- Lutein - Zeaxanthin
- Glucosamine - Chondroitine
- Omega-3
The Frost & Sullivan Case Studies have several objectives:
Critically examine the scientific literature on dietary supplements for which there is an as-
sociation between the absorption of the supplement and the reduction of the risk of contracting
the disease that can lead to loss of autonomy, hospital stays as well as a lack of productivity
Make an estimate of the potential healthcare savings that may result from daily use of the
dietary supplement
Determine the value of the net economic benefit of targeted supplement intake, which has been
shown to inhibit the onset of a given pathology
Determine the total potential savings on health burden resulting from the use of dietary sup-
plements versus health management expenses
Keywords: healthcare, cost savings, dietary supplements, France
∗
Speaker
†
50
Pharmacognosy and safety of food
supplements
∗ 1,2
Caroline Rouger , Pierre Waffo-Teguo 2 , Julien Gabaston 2
1
2
Univ. de Bordeaux, UFR des Sciences Pharmaceutiques, Unité de recherche Oenologie, EA 4577, USC
1366 INRA – Université de Bordeaux (Bordeaux, France) – Institut des Sciences de la Vigne et du Vin
(ISVV) 210 Chemin de Leysotte, CS 50008, F-33882 Villenave d’Ornon, France
Pharmacognosy studies raw materials and substances from biological origin with a therapeu-
tic aim. This interdisciplinary science is fundamental in pharmaceutical education and covers
a wide range of natural sources including plants and fungi. Although food supplements are
intended to possess a nutritional or physiological effect, they may be composed of traditional
medicinal plants containing specialised metabolites with potential adverse effects. In addition,
fraudulent herbal supplements subjected to adulteration or substitution can be found on the
market and can seriously harm human health. Safety and quality of botanicals and derived
preparations found in food supplements must therefore be assessed in an organised and scien-
tific manner. In this view, the European Food Safety Authority provided guidelines towards this
safety assessment. Pharmacognostic study of medicinal plants is here highly relevant since it
allows standardisation and authentication of plant species through a combination of evaluation
parameters involving botanical examination and chemical reactions.
Keywords: Medicinal plants, Quality control, Standardisation, Analytical methods
∗
Speaker
51
The place of food supplements and
medicinal plants in the health system
∗ 1
Michel Tourrasse
1
Hippocratus – Ministère de la santé – 22 bis allées Turcat Méry Immeuble Circé, 13008 Marseille,
France
Talk by Dr Michel Tourrasse, Medical doctor, herbalist, aromatherapist, teacher founder of

the Hippocratus course.
The synthetic drug prescription as the first line treatment is justified only in two cases: 1) It is
an emergency, 2) there is no natural option.
Vitamins, minerals, medicinal plants, essential oils, bees deriving products, share 80% of my
current prescriptions in rheumatology, preventive infectiology, gastro-enterology, dermatology,
etc. as well as in the psycho-affective issues.
There usage can reduce the global health costs and to reduce the drug iatrogenicity that counts
for more than 10 000 death per year in France. It can also decrease the environmental pollution.
Keywords: santé, plantes médicinales, compléments alimentaires, prescription, indications
∗
Speaker
52
Author Index
Aja Perez, Iris, 24 Faure, Chrystel, 17

Alberts, Isabell, 25 Feracci, Ugo, 52
Amaral, Joana, 41 Feurtet-Mazel, Agnès, 8
Assemat, Gaëtan, 42, 44, 46 Flandin, Amélie, 29, 31, 33
Atgié, Claude, 17 Fouillen, Laetitia, 25, 33
Franc, Céline, 11, 22
BALAYSSAC, Stéphane, 42–44, 47
Balayssac, Stéphane, 46 GABASTON, Julien, 19, 22, 27, 53
Bardot, Valérie, 40 Gamba, Donatien, 52
BEAUMONT, Pauline, 17 Gaudin, Jonathan, 13
BELLENOT, Denis, 12 Geneste, Nathalie, 8
BENNETAU, Catherine, 6 Ghazi, Irfan Ahmad, 34
Bernillon, Stéphane, 29, 33 Gibon, Yves, 29, 31, 33
Berthomier, Lucile, 40 Gilard, Véronique, 42–44, 46, 47
Boutié, Philippe, 15, 16 Gougeon, Louis, 48
Burzdiej, Aleksandra, 20 Gudipalli, Padmaja, 10
Gutiérrez, Rodrigo A., 29
Cabasson, Cécile, 33 Guyon, François, 48
CABASSON, Cecile, 6
Cassan, Cédric, 29, 31, 33 Hachem, Rabab, 42, 44
Cavaignac, Sebastien, 15, 16 Hastoy, Cécile, 13, 15, 16
Chapteuil, Christelle, 51 HERNOULD, Michel, 6, 14
Chauvigné, Emilie, 50
CHEVALIER, Christian, 14 Iglesias, Marie-Laure, 27
Chevanne, Chloé, 31 Jacob, Daniel, 33
Cluzet, Stéphanie, 20, 27 Jagota, Anita, 37
Cookson, Sarah J, 22
Cosson, Patrick, 13, 15, 16 Kadiri, Camélia Feyrouse, 31
Courtois, Arnaud, 17, 24 Krisa, Stéphanie, 17, 24
Da Costa, Grégory, 20, 33, 48 LAFAY, SOPHIE, 35

Danoun, Saı̈da, 43, 47 Le Bihan, Zoé, 13, 15, 16
De Revel, Gilles, 11 Le Mao, Ines, 48
Deborde, Catherine, 33 Leblanc, Anne, 40
Decendit, Alain, 24, 27 Liesnieswska, Eric, 25
Deleu, Magali, 25 Lins, Laurence, 25
Delmas, Frederic, 14 Loupit, Grégoire, 22
Djemaa-Landri, Kenza, 19, 20 Luna, Estrella, 31
Dopter, Aymeric, 39
Douillet, Antonin, 20 Maes, Emmanuel, 25
Dubourdeaux, Michel, 40 MALET-MARTINO, Myriam, 42–44, 47
Dussarrat, Thomas, 29 Malet-Martino, Myriam, 46
Mamode Cassim, Adiilah, 25
EL KHAWAND, TONI, 24, 27 Mandou, Claire, 20
MARTIN, Ambroise, 49
53
Martino, Robert, 42, 44
Martins-Froment, Nathalie, 42
MERILLON, Jean-Michel, 6, 19, 27
Moing, Annick, 33
Mongrand, Sébastien, 25, 33
Mueller, Constanze, 30
Mukherjee, Pulok K., 28, 38
Nuernberger, Thorstern, 25
Okeke, Ifunanya, 47
Pédrot, Eric, 27, 33

Pétriacq, Pierre, 29, 31, 33
Pagès, Guilhem, 44
Petitet, François, 36
Prigent, Sylvain, 29, 33
Raghavendra, Agepati, 38
Raghavendra, Agepati S, 28
RENOUF, Elodie, 45
Renouf, Elodie, 33
Richard, Tristan, 19, 22, 24, 27, 33, 48
Rolin, Dominique, 13, 15, 16, 29, 33
Rouger, Caroline, 19, 33, 53
Schurdi-Levraud, Valérie, 13, 15, 16

Sclaunich, Eric, 15, 16
Taillis, David, 27
Tetali, Sarada, 28, 38
Tourrasse, Michel, 54
Valls Fonayet, Josep, 19, 20, 22

Valls, Josep, 24, 27, 33
van Delft, Pierre, 33
Vu, Marion, 20
Wójtowicz-Zawadka, Marzena, 46
Waffo-Teguo, Pierre, 19, 53
Woch, Nyanthanglo, 10
Wu, Nao, 43
54
List of participants
55 p. 1
Name Surname Origin e-mail contact
1 Etu Abdel-Malak Stéphanie Master 2 Stratégies Juridiques [email protected]

et Economiques de Mise sur le
Marché des Produits de Santé
2 Agepati Raghavendra Department of Plant Sciences, [email protected]

School of Life Sciences,
University of Hyderabad
3 Etu Al Nahhas Dina Master contrôle qualité et [email protected]

développement analytique des
médicaments naturels
4 Etu Ali-abargooi Roxane Master 2 Stratégies Juridiques [email protected]

5 Etu Alos Julie Parcours Plantes à Valeur [email protected]

Santé et Biomolécules d'Intérêt
M2
6 Amaral Joana Polytechnic Institute of [email protected]

Bragança, Lisboa Portugal
7 Aubert Guillaume ICMCB [email protected]
8 Bacles Cécile University of Bordeaux, Projet [email protected]

setting service
9 Bakoma Batomayena Dept [email protected]

Pharmacy/Pharmacognosy,
Université de Lomé, Togo
10 Balayssac Stéphane Université de Toulouse III - [email protected]

Paul Sabatier
11 Barde Alexia Global Products Solutions
12 Etu Barry Kadiatou Master contrôle qualité et [email protected]

13 Etu Baury Aurore Bordeaux Sciences Agro, [email protected]

Nutrition Santé, Nexira
56 p. 2
14 Etu Beaumont Pauline Team MIB, ISVV, University [email protected]

of Bordeaux
15 Bellenot Denis iteipmai [email protected]
15 Bennetau Catherine ARNA Inserm Unit 1212, [email protected]

Pharmacy Faculty, University
of Bordeaux, Bordeaux
Sciences Agro
16 Etu Bensaada Souad Parcours Plantes à Valeur [email protected]
M2
17 Berthomier Lucile Pileje industrie
18 Etu Biyoghe Maylis Parcours Plantes à Valeur [email protected]

M2
19 Etu Bonnard Mélanie Master 2 Chimie Organique et [email protected]

Sciences du vivant
20 Etu Bourhani Haya Master contrôle qualité et [email protected]

21 Chapteuil Christelle Synadiet / Juva santé [email protected]
22 Charrouf Zoubida Department of Chemistry, [email protected]

Université Mohammed V,
Rabat, Morocco
23 Chaudière Jean University of Bordeaux [email protected]

Laboratoire CBMN – UMR
5248
24 Chauvigné Emilie Valbiotis [email protected]
25 Chethala N Vishnuprasad The University of Trans- [email protected]

Disciplinary Health Sciences
and Technology (TDU)
26 Danaux Stéphanie PhD internationalization service [email protected]
57 p. 3

27 Danoun Saïda Université Paul Sabatier [email protected]
Laboratoire SPCMIB-RMNBio
28 Debroczi Caroline Santé Verte Ltd Succursale

France
29 Delmas Fred Biologie du fruit et pathologie [email protected]

Inra : UMR1332, University of
Bordeaux
30 Etu Decros Guillaume Biologie du fruit et pathologie [email protected]

Bordeaux
31 Etu Descalis Sabran Marie Master 2 Stratégies Juridiques [email protected]

32 Etu Destailleur Alice Biologie du fruit et pathologie [email protected]

Bordeaux
33 Dévier Marie-Hélène LPTC, UMR 5805 EPOC, [email protected]

CNRS, University of Bordeaux
34 Dopter Aymeric Anses
35 Dubourdeau Michel Pilèje
36 Dussarrat Thomas Biologie du fruit et pathologie [email protected]

Bordeaux
37 El Khawand Toni ISVV, University of Bordeaux [email protected]

Unité de recherche Oenologie,
EA 4577, USC 1366 INRA
38 Farache Gerard TOWER FARM R&D [email protected]
39 Feracci Ugo Frost & Sullivan [email protected]
58 p. 4

40 Ferrier Manon Master Plantes à Valeur Santé [email protected]
et Bioloécules d'Intérêt Sortant
41 Etu Flochlay Justine Master Toxicologie et [email protected]

Ecotoxicologie M2
42 Fouillen Laetitia Laboratoire de Biogenese [email protected]

Membranaire Inra, UB
43 Franc Céline Unité de Recherche Œnologie, [email protected]

EA 4577, USC 1366 INRA,
ISVV, Université de
44 Gadeau Alain-Pierre Department of Biology and [email protected]

health
45 Gakière Bertrand Université Paris Sud, Plant [email protected]

Sciences of Saclay,
Metabolome platform, SPOmic
SPS2, Gif sur Yvette
46 Gamba Donatien Frost & Sullivan
47 Geneste Nathalie Université de Bordeaux - UMR [email protected]

EPOC 5805 CNRS - LPTC
48 Genthon Lydia Naturenvie - Groupe Léa [email protected]

Nature
49 Ghazi Irfan Ahmad Department of Plant Sciences, [email protected]

50 Gisbert Aurélie Nexira
51 Goron Arthur Laboratoires Juva Santé [email protected]
52 Goujon Thomas Inra, Reproduction et [email protected]

développement des plantes-
UMR 5667- RDP
59 p. 5

53 Gudipalli Padmaja Department of Plant Sciences, [email protected]
54 Guichard Gilles Department of Sciences and [email protected]

Technologie for health
55 Etu Guillot Mathilde Master 2 Stratégies Juridiques [email protected]

56 Etu Guindeuil Nautila Master Toxicologie et [email protected]

Ecotoxicologie M1
57 Hassoune Mehdi Ircos Laboratoires [email protected]
58 Hernould Michel Biologie du fruit et pathologie [email protected]

Bordeaux
59 Hornn Ophelie Master Plantes à Valeur Santé [email protected]

et Bioloécules d'Intérêt sortant
60 Etu Itani Mohamad Master contrôle qualité et [email protected]

61 Jagota Anita Department of Animal Biology, [email protected]

62 Joubert Margot Global Products Solutions
63 Etu Karim Mariame Master contrôle qualité et [email protected]

64 Etu Kayamare Mégane Master international [email protected]

AgroBioMed GIP Triad
65 Labro Julie Nexira [email protected]
60 p. 6

66 Lafay Sophie GYNOV [email protected]
67 Etu Lamhidra Oumaima Master contrôle qualité et [email protected]

68 Etu Lasri El-Batoul Master 2 Plants with health [email protected]

benefits
69 Etu Laurençon Marianne Master Biologie Agrosciences [email protected]
70 Lautard Elodie Global Products Solutions
71 Le Bihan Zoé UMR INRA Biologie du Fruit [email protected]

et Pathologie Doctorante
72 Le coq-Sarrazin Déborah Laboratoire Phyto-Actif
73 Lecroart Pascal Sciences and Technology [email protected]

College
74 Etu Lefevre Camille Master 2 Stratégies Juridiques [email protected]

75 Leichtnam marie-laure Laboratoires GILBERT
76 Le Mao Ines ISVV, University of Bordeaux [email protected]

Unité de recherche Oenologie,
EA 4577, USC 1366 INRA
77 Etu Lesbazeilles Apolline Parcours Plantes à Valeur [email protected]

M2
78 Loffredo Loic BotaniCERT [email protected]
61 p. 7

79 Etu Loupit Grégoire Biologie du fruit et pathologie [email protected]
Bordeaux
80 Magnier Justine Global Products Solutions
81 Mahrouz Mostafa Université Cadi Ayyad, Faculté [email protected]

des Sciences Semlalia,
Marrakech
82 Malet-Martino Myriam SPCMIB, Université Paul [email protected]

Sabatier, Toulouse
83 Mallon Moure Ruben Nexira [email protected]
84 Martin Ambroise University of Lyon
85 Millet Philippe Editions Business Group Media [email protected]
86 Etu Moreau Felix Master Toxicologie et [email protected]

Ecotoxicologie M1
87 Mounib Myriam Master Nutrition - U. Bordeaux [email protected]

DU Compléments Alimentaires
: Sécurité, Efficacité,
Innovation
88 Etu Mutelet Elise Master 2 Stratégies Juridiques [email protected]
89 Nadimpalli Siva Kumar Biochemistry Department, [email protected]

90 Etu Offoumou Rostand Parcours Plantes à Valeur [email protected]

M2
91 Etu Ouadia Naila Master contrôle qualité et [email protected]

62 p. 8

92 Pagèse Joanne University of Bordeaux Vice [email protected]
President of International
affairs for education
93 Petit Eva Doctorat en développement des [email protected]

Plantes INP de Toulouse
94 Petit Léna Master Plantes à Valeur Santé [email protected]

95 Petitet François Herbéo [email protected]
96 Pétriacq Pierre Plateforme MetaboHUB- [email protected]

Bordeaux, Biologie du fruit et
pathologie UMR Inra 1332
97 Podile Appa Rao Vice Chancellor of the [email protected]
98 Pommet Jean-Michel Innovation in Nutrition [email protected]
99 Etu Queuille Rémi Master Toxicologie et [email protected]

Ecotoxicologie M1
100 Etu Rahim Abdelhak Master contrôle qualité et [email protected]

101 Rahni Sonia Laboratoire Phyto-actif
102 Etu Rajaonarison Laura Master 2 Stratégies Juridiques [email protected]

103 Renouf Elodie ADERA - Polyphenol Biotech [email protected]
104 Rhourri Frih Boutayna CBMN UMR5248, University [email protected]

of Bordeaux
63 p. 9

105 Richard Leo Global Products Solutions
106 Richard Tristan Team MIB, ISVV, University [email protected]

of Bordeaux
107 Ripoll Christophe Natinov
108 Etu Roger Erika Parcours Plantes à Valeur [email protected]

M2
109 Rolin Dominique Plateforme MetaboHUB- [email protected]

Bordeaux, Biologie du fruit et
pathologie UMR Inra 1332
110 Rosebery Juliette Irstea Bordeaux, Aquatic [email protected]
Ecosystems and Global Change
111 Rouger Caroline Faculty of pharmacy, Enology [email protected]

RU, Univ. Bordeaux
112 Ruaud Gautier Parcours Plantes à Valeur [email protected]

sortant
113 Saad Naima Laboratoire PEIRENE EA [email protected]

7500, Université de Limoges
114 Etu Salamero Marta Master contrôle qualité et [email protected]

115 Etu Sani Malam Master contrôle qualité et [email protected]

116 Schurdi-Levraud Valerie Biologie du fruit et pathologie, [email protected]

Inra UMR1332, Université de
Bordeaux
117 Etu Sekkat Abla Master contrôle qualité et [email protected]

64 p. 10

118 Etu Sigogne Celia Parcours Plantes à Valeur [email protected]
M2
119 Silva Maria Laura University of Bordeaux, School [email protected]

of Pharmacy · Pharmaceutical
Law and Economics
120 Szlosek Pinaud Magali Director of the Master Chimie [email protected]

organique et Sciences du vivant
121 Etu Stratis Alix Master 2 Stratégies Juridiques [email protected]

122 Swamy Musti School of Chemistry, [email protected]

123 Etu Sy Biranti Parcours Plantes à Valeur [email protected]

M2
124 Taillis David Laboratoire MIB, ISVV, [email protected]

Université de Bordeaux
125 Tetali Sarada Department of Plant Sciences [email protected]

126 Tourrasse Michel Hippocratus, Montpellier [email protected]
127 Etu Ulatowski Oxana Parcours Plantes à Valeur [email protected]

M2
128 Valls Josep Institut des Sciences de la [email protected]

Vigne et du Vin
129 Veyret Elodie Synadiet [email protected]
130 Viaud Esther Master Plantes à Valeur Santé [email protected]

65 p. 11

131 Vishnuprasad Chethala N Centre for Ayurveda Biology [email protected]
and Holistic Nutrition, Trans-
disciplinary University of
Health and Technology
132 Vorobieva Marina Q-Source [email protected]
133 Etu Wantz Baptiste Master 2 Stratégies Juridiques [email protected]

134 Etu Windels Marie-Timothée Master 2 Stratégies Juridiques [email protected]

135 Etu Xinshua Liu Master contrôle qualité et [email protected]

136 Etu Zheng Jean-Elie Master 2 Chimie Organique et [email protected]

Sciences du vivant
137 Etu Zilber Louise Master Toxicologie et [email protected]

Ecotoxicologie M1
66 p. 12

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Plants with health benefits and Biomolecules of Interest University of Bordeaux 27 – 29 Nov 2019

2nd Indian-French Symposium

LUNCH 01:00 pm – 02:00 pm

4:30 pm -5:00 pm Pause / Poster session

Thursday 28th of November 2019 – 8:00 am

10:00 am – 10:30 am Pause / Poster session

Lunch 12:30 pm - 02:00 pm

Departure for Social Event 05:30 pm

Friday 29th of November 2019 08:30 am

10:30 am – 11:00 am Pause / Poster award ceremony

Lunch 12:30 am - 01:30 pm

03:30 pm – 04:00 pm Pause/Transportation

04:00 pm – 06:00 pm: Platform visit CGFB facilities

An Erasmus Mundus accreditation for the Environmental Contamination and

Pesticide analysis in food, Céline Franc [et al.] . . . . . . . . . . . . . . . . . . . . 9

Screening for Stevia rebaudiana response to Septoria Leaf Blotch in controlled

The TomGEM project: Effects of elevated temperatures on floral meristem initi-

Evaluation of Stevia rebaudiana Genotypes under Field Conditions in the South

Development of Stevia rebaudiana production in Europe: Germplasm evaluation

Encapsulation of trans--viniferin in multi-lamellar liposomes, Pauline Beaumont [et

Phenolic compounds from grapevine leaves: methods of extraction and analy-

Composition and antioxidant activities of stilbenes cane extracts from different

Identification of oligomeric stilbenes produced by oxidative coupling of resveratrol

Multipolar approach to decipher Glycosyl Inositol Phosphoryl Ceramides’ (GIPCs)

Oligomeric stilbenes produced by oxidative coupling of resveratrol show high

Plant metabolomics: A tool for authentication of medicinal plant products, Sarada

Exploring metabolome in extremophile plants from Atacama desert, Thomas Dus-

Metabolomics to exploit the primed immune system of tomato fruit, Estrella

MetaboHUB-Bordeaux Facility, Stéphane Bernillon [et al.] . . . . . . . . . . . . . 31

Therapeutic biomolecules from plants with anticancer properties, Irfan Ahmad

Plant-based food supplement formulation in Europe, Sophie Lafay . . . . . . . . 33

The mechanism of plants & drug interactions, François Petitet . . . . . . . . . . 34

Age induced Circadian Rhythm dysfunction in Central and Peripheral Clocks :

Metabolite based evaluation and quality control of spices, Agepati Raghaven-

The French Nutrivigilance scheme, Aymeric Dopter . . . . . . . . . . . . . . . . . 37

DNA-based approaches for the botanical authentication of medicinal plants and

1H NMR versus UHPLC-DAD-MS chemical profiling of red yeast rice dietary

Evaluation of low-field proton NMR spectroscopy for quality control of cinnamon

High-field and low-field NMR for unveiling adulteration of dietary supplements

Polyphenols Biotech transfer unit, Elodie Renouf . . . . . . . . . . . . . . . . . . 43

Detection and quantification of stimulants in supplements for athletes by 1H

Quality control of active compounds (icariins) in Epimedium grandiflorum herbal

Quantitative analysis of wine composition applied to Bordeaux red wines by 1H-

European and French regulations on claims, Ambroise Martin . . . . . . . . . . . 47

The Canadian Regulation - Natural Health Products, Emilie Chauvigné . . . . . 48

Pharmacognosy and safety of food supplements, Caroline Rouger [et al.] . . . . . 51

Launching in September 2020, the Environmental Contamination and Toxicology

Plants produce an array of secondary metabolites as a means of adaptation to environmen-

Pesticides, specifically plant protection products (PPPs), also known as phytopharmaceuti-

Keywords: pesticide residues, food, wine, analytical methods

Keywords: iteipmai, medicinal plant, quality, variability

Keywords: S. rebaudiana, Septoria Leaf Blotch, response screening

Keywords: Climate change, tomato, heat stress, quality

Cécile Hastoy 1 , Zoé Le Bihan ∗† 1,2 , Patrick Cosson 2 , Eric Sclaunich 3 ,

In order to establish an organic Stevia rebaudiana production in South West of France,

Keywords: Stevia rebaudiana, genotype, field experiment, organic production

Cécile Hastoy 1 , Zoé Le Bihan ∗ 1,2 , Patrick Cosson 2 , Sebastien

Keywords: Stevia rebaudiana, genotype, field experiment, microsatellites

Josep Valls Fonayet ∗ 1 , Julien Gabaston 1 , Caroline Rouger 1 , Kenza

Encapsulation of trans--viniferin in multi-lamellar liposomes, Pauline Beaumont [et