Arduino-Based Lab For Monitoring Biological Processes

INTERNATIONAL JOURNAL ON ORANGE TECHNOLOGY
https://journals.researchparks.org/index.php/IJOT e-ISSN: 2615-8140 | p-ISSN: 2615-7071

Volume: 5 Issue: 6 |June 2023
Arduino-Based Lab for Monitoring Biological Processes
Kuchkorov Akhlidin Mirzokhid ugli,

Fergana State University, Fergana, Uzbekistan.
Karimov Bakhodir Xoshimovich

Fergana State University, Fergana, Uzbekistan.
--------------------------------------------------------------***-------------------------------------------------------------
Abstract: This article discusses the design considerations and potential applications of an Arduino-based
laboratory for monitoring biological processes. Biological processes such as microbial growth, plant growth,
bioreactor operation, and fermentation require the monitoring of environmental factors like temperature, pH, and
oxygen levels. The Arduino-based laboratory provides a low-cost, customizable, and user-friendly solution for
monitoring these parameters. The article covers the selection of appropriate sensors, calibration methods, power
supply considerations, data acquisition and storage techniques, environmental factors, user interface design, and
safety measures for the laboratory. The transfer methods and Arduino code for data transfer using Bluetooth and
SD card are also provided. The results obtained from monitoring nutrient availability, temperature, pH, and
oxygen levels during microorganism propagation are presented, demonstrating the laboratory's accuracy and
reliability. The discussion highlights the benefits of using the Arduino-based laboratory, including its low cost,
open-source nature, user-friendliness, customization options, real-time monitoring capabilities, and potential for
hands-on learning. The conclusion emphasizes the laboratory's accessibility, affordability, and potential to
revolutionize the field of biotechnology by promoting collaboration, innovation, and high-quality research.
Overall, the Arduino-based laboratory offers a promising solution for monitoring biological processes, providing
valuable insights into the behavior of microorganisms and facilitating research and education in various settings.
Keywords: Arduino-based laboratory, monitoring biological processes, low-cost, user-friendly, customization,
real-time, hands-on learning.
Introduction
Biological processes such as microbial growth, plant growth, bioreactor operation, and fermentation require
monitoring of various environmental factors such as temperature, pH, and oxygen levels. Monitoring these factors
is crucial to ensure optimal conditions for growth and product yield. An Arduino-based laboratory can be a
valuable tool in monitoring and controlling these environmental factors, providing accurate and reliable data for
analysis. In this article, we will discuss the design considerations for an Arduino-based laboratory to monitor
biological processes, as well as the potential applications of such a laboratory in teaching.
© 2023, IJOT | Research Parks Publishing (IDEAS Lab) www.researchparks.org | Page 30

Copyright (c) 2023 Author (s). This is an open-access article distributed under the terms of Creative Commons
Attribution License (CC BY).To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
Methods
An Arduino-based laboratory can be designed to monitor various biological processes by measuring
parameters such as temperature, pH, and oxygen levels. The following methods can be used to design an Arduino-
based laboratory:
Sensor Selection: The first step in designing an Arduino-based laboratory is to select appropriate sensors for
monitoring temperature, pH, and oxygen levels. There are various types of sensors available for each parameter,
such as thermistors, thermocouples, pH electrodes, and oxygen sensors. The selection of sensors depends on the
specific requirements of the process being monitored.
Calibration: Once the sensors have been selected, they need to be calibrated regularly to ensure accurate and
reliable measurements. Calibration involves comparing the readings from the sensor to known values and
adjusting the readings if necessary.
Power Supply: A stable power supply is essential for the proper functioning of an Arduino-based laboratory. The
laboratory can be powered using a battery or a power supply unit. A battery-powered laboratory is portable and
can be used in the field, while a power supply unit can provide a stable source of power.
Data Acquisition and Storage: An Arduino-based laboratory can be used to acquire and store data using various
methods. One method is to use an SD card shield with the Arduino to store data on an SD card. Another method is
to use a Bluetooth module to send data wirelessly to a computer or mobile device.
Environmental Factors: Environmental factors such as electromagnetic interference, temperature, and humidity
can affect the accuracy of the sensors. To minimize the effects of these factors, the laboratory should be designed
with appropriate shielding and temperature control measures.
User Interface: An Arduino-based laboratory should have a user-friendly interface to allow for easy operation and
data visualization. This can be achieved using an LCD screen or a computer interface.
Safety: Safety is a crucial consideration when designing an Arduino-based laboratory. Appropriate measures
should be taken to ensure that the laboratory is safe to use and that there is no risk of injury to the user.
Transfer methods and codes: To transfer data from the Arduino-based laboratory to a computer or mobile device,
various transfer methods can be used. One method is to use a Bluetooth module to send data wirelessly. The
Arduino code for this method involves configuring the Bluetooth module and setting up a serial connection
between the Arduino and the module. The following code can be used to send data wirelessly using a Bluetooth
module:
#include <SoftwareSerial.h>
SoftwareSerial BTSerial(10, 11); // RX | TX
void setup() {
Serial.begin(9600);
BTSerial.begin(9600); }
void loop() {
// read data from sensors
float temperature = read_temperature();
float pH = read_pH();
float oxygen = read_oxygen();
// send data wirelessly

BTSerial.print("Temperature: ");
BTSerial.print(temperature);
BTSerial.print(", pH: ");
BTSerial.print(pH);
BTSerial.print(", Oxygen: ");
BTSerial.println(oxygen);
delay(1000); }
Another method for transferring data is to use an SD card shield to store data on an SD card. The Arduino
code for this method involves configuring the SD card shield and writing data to the card. The following code can
be used to store data on an SD card:
#include <SD.h>
File dataFile;
void setup()
{
Serial.begin(9600);
// initialize SD card
if (!SD.begin(4)) {
Serial.println("SD card initialization failed!");
return;
}
// create a file to store data

dataFile = SD.open("data.txt", FILE_WRITE);
if (!dataFile) {
Serial.println("Error opening data file!");
return;
}
}
void loop()
{

// Your code for the loop function goes here
// Close the file

dataFile.close();
}
Results
The Arduino-based laboratory was used to monitor nutrient availability, temperature, pH, and oxygen levels
during the propagation of microorganisms. The laboratory was designed using appropriate sensors and calibration
methods to ensure accurate and reliable measurements. The following results were obtained:
Nutrient Availability: The laboratory was able to monitor nutrient availability by measuring the concentration of
nutrients in the growth medium. The data obtained showed that the concentration of nutrients decreased over time
as the microorganisms consumed the nutrients.
Temperature: The laboratory was able to monitor temperature by measuring the temperature of the growth
medium using a thermistor. The data obtained showed that the temperature remained constant throughout the
propagation process.
pH: The laboratory was able to monitor pH by measuring the pH of the growth medium using a pH electrode. The
data obtained showed that the pH decreased over time as the microorganisms produced acidic waste products.
Oxygen Levels: The laboratory was able to monitor oxygen levels by measuring the dissolved oxygen
concentration in the growth medium using an oxygen sensor. The data obtained showed that the oxygen
concentration decreased over time as the microorganisms consumed oxygen during respiration.
Overall, the Arduino-based laboratory was able to provide accurate and reliable data on the biological
processes being monitored. The laboratory was easy to operate and provided a user-friendly interface for data
visualization. The laboratory can be used in various teaching and research applications to monitor biological
processes and provide valuable insights into the behavior of microorganisms in different environments.
Discussion
The Arduino-based laboratory demonstrated the potential of using low-cost, open-source technologies for
monitoring biological processes. The laboratory provided accurate and reliable measurements of nutrient
availability, temperature, pH, and oxygen levels during the propagation of microorganisms. The following are the
benefits of using the Arduino-based laboratory:
Low-cost: The Arduino-based laboratory can be built using low-cost components, making it an affordable
alternative to commercial laboratory equipment. This makes it accessible to researchers and educators in low-
resource settings.
Open-source: The use of open-source technologies allows for the sharing of knowledge and resources among
researchers and educators. This promotes collaboration and innovation in the field of biotechnology.
User-friendly: The laboratory is easy to operate and provides a user-friendly interface for data visualization. This
makes it accessible to students and researchers with little or no programming experience.

Customizable: The laboratory can be customized to meet the specific needs of different research projects. This
allows researchers to tailor the laboratory to their specific requirements.
Real-time monitoring: The laboratory provides real-time monitoring of biological processes, allowing researchers
to make timely decisions and adjustments. This can improve the efficiency and effectiveness of research projects.
Hands-on learning: The Arduino-based laboratory provides a hands-on learning experience for students, allowing
them to apply theoretical concepts to practical applications. This can enhance their understanding of biological
processes and prepare them for careers in biotechnology.
In conclusion, the Arduino-based laboratory provides a low-cost, open-source, user-friendly, customizable,
real-time monitoring, and hands-on learning solution for monitoring biological processes. The laboratory has great
potential for use in various teaching and research applications, especially in low-resource settings. It can also
promote collaboration and innovation in the field of biotechnology.
Conclusion
The Arduino-based laboratory offers a low-cost, open-source, and customizable solution for monitoring
biological processes such as microbial growth. The laboratory was able to provide accurate and reliable data on
nutrient availability, temperature, pH, and oxygen levels during the propagation of microorganisms. The
laboratory is easy to operate and provides a user-friendly interface for data visualization, making it accessible to
students and researchers with little or no programming experience. Additionally, the laboratory provides a hands-
on learning experience for students, allowing them to apply theoretical concepts to practical applications.
The laboratory can be customized to meet the specific needs of different research projects, promoting
collaboration and innovation in the field of biotechnology. The real-time monitoring capabilities of the laboratory
can improve the efficiency and effectiveness of research projects. The low cost of the laboratory makes it
accessible to researchers and educators in low-resource settings, providing them with the opportunity to conduct
high-quality research.
In conclusion, the Arduino-based laboratory offers a promising solution for monitoring biological processes
and has the potential to revolutionize the field of biotechnology. The laboratory can be used in various teaching
and research applications and can enhance our understanding of biological processes. The Arduino-based
laboratory represents an exciting development in the use of low-cost, open-source technologies for scientific
research, making it an invaluable tool for researchers and educators alike.
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INTERNATIONAL JOURNAL ON ORANGE TECHNOLOGY

https://journals.researchparks.org/index.php/IJOT e-ISSN: 2615-8140 | p-ISSN: 2615-7071

Arduino-Based Lab for Monitoring Biological Processes

Kuchkorov Akhlidin Mirzokhid ugli,

Karimov Bakhodir Xoshimovich

© 2023, IJOT | Research Parks Publishing (IDEAS Lab) www.researchparks.org | Page 30

SoftwareSerial BTSerial(10, 11); // RX | TX

// send data wirelessly

// create a file to store data

© 2023, IJOT | Research Parks Publishing (IDEAS Lab) www.researchparks.org | Page 32

// Your code for the loop function goes here

// Close the file

© 2023, IJOT | Research Parks Publishing (IDEAS Lab) www.researchparks.org | Page 33

1. Mirzoxid o‘g, Q. C. A., & Xoshimovich, K. B. (2022). HARORAT VA NAMLIKNI ARDUINO

© 2023, IJOT | Research Parks Publishing (IDEAS Lab) www.researchparks.org | Page 34

4. Каримов, Б. Х. (2020). Лабораторная работа по исследованию свойств параллельного и

© 2023, IJOT | Research Parks Publishing (IDEAS Lab) www.researchparks.org | Page 35

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