XS Series E 02 Hydraulics

SECTION 2 HYDRAULICS
2.1 PNEUMATIC SYSTEM........................................................................................................................1
2.2 USE OF PRESSURE AND VACUUM .................................................................................................2
2.3 HYDRAULIC SYSTEM ........................................................................................................................3

2.3.1 RBC/PLT Analysis Procedure ............................................................................................5
2.3.2 Hgb Analysis Procedure ....................................................................................................6
2.3.3 Computing the Erythrocyte Indices ....................................................................................6
2.3.4 WBC Classification (CBC+DIFF mode) .............................................................................7
2.3.5 WBC Classification (CBC mode) .......................................................................................8
2.4 ANALYSIS PARAMETER ....................................................................................................................9
2.5 REAGENT SYSTEM .........................................................................................................................10
2.6 ANALYSIS FLOW..............................................................................................................................11
2.7 FCM DETECTOR BLOCK.................................................................................................................12

2.7.1 Function ...........................................................................................................................13
2.8 OPTICAL SYSTEM ...........................................................................................................................15
2.9 HYDRAULIC PARTS .........................................................................................................................16

2.9.1 Solenoid Valve Function ..................................................................................................16
2.9.2 HYDRAULIC PARTS LOCATION ....................................................................................18
2.10 HYDRAULIC SEQUENCE DESCRIPTION .....................................................................................23
XS Series S/M December 13, 2007

SECTION 2 HYDRAULICS
2.1 PNEUMATIC SYSTEM
The pneumatic system needed for XS operation consists of 2 pneumatic outputs, +0.06Mpa pressure,
and -0.03Mpa vacuum. These pneumatic outputs are generated by built-in air pump unit and regulated to
+0.06 Mpa and -0.03 Mpa by corresponding relief valves respectively. These relief valves have no
adjustment points.
Figure2-3-0-1: XS Pneumatic System
XS Series S/M 2-1 September 2016

2.2 USE OF PRESSURE AND VACUUM
Uses of pneumatic outputs for XS are as follows.
Type Functions
Pressure Driving diaphragm pumps, To form Sheath Flow,
0.06MPa Draining Pressure for Waste Chambers
Driving air cylinder in Sampler Unit
Vacuum Driving diaphragm pumps, Replenishing EPK Chamber,
-0.03MPa Draining Vacuum for Waste Chambers
Driving air cylinder in Sampler Unit

2.3 HYDRAULIC SYSTEM
(1) Whole Blood Mode
Figure 2-3-0-1: Hydraulic System Flow in Whole Blood Mode

(2) Capillary Mode
Figure 2-3-0-2: Hydraulic System Flow in Capillary Mode

2.3.1 RBC/PLT Analysis Procedure
The procedure for analyzing RBC/PLT is explained here.
Figure 2-3-1: RBC/HGB Analysis Procedure
(1) Sample Aspiration Probe aspirates whole blood (or diluted sample for capillary mode) .
(2) 4.0µL of whole blood (9.0µL of diluted sample for capillary mode) is diluted with 2.0mL of EPK
(CELLPACK) in the RBC Chamber.
(3) The Sheath Syringe injects 10.3µL of diluted sample slowly to the RBC/PLT detector while RBC
sheath flow is formed.
(4) The RBC detector counts the RBC and PLT via the Hydro Dynamic Focusing (DC Detection). At
the same time, the hematocrit (HCT) is calculated via the RBC pulse height detection method.

2.3.2 Hgb Analysis Procedure
The procedure for analyzing HGB is explained here.
Figure 2-3-2: Hgb Analysis Procedure
(1) After RBC/PLT analysis, 0.5mL of SULFOLYSER is added to 1.0 mL of diluted sample remained in
RBC/HGB chamber, diluting to 751 times (2340 times for capillary mode), hemolyzing red blood
cells, and converts hemoglobin to SLS-Hemoglobin.
(2) The diluted sample in the step 1 is charged into the HGB flow cell.
(3) The concentration of SLS-hemoglobin is measured as light absorbance, and is calculated by com-
parison with the absorbance of the Hgb blank level measured in step2.
2.3.3 Computing the Erythrocyte Indices

The red blood cell constants (mean cell volume, mean cell hemoglobin, and mean cell hemoglobin
concentration) are calculated from the RBC, HGB, and HCT.
(1) Mean cell volume (MCV)

The MCV is calculated from the RBC and HCT, using the following equation:
HCT(%)
MCV  fL  = ----------------------------------------x10
6
RBC(x10 /L)
(2) Mean cell hemoglobin (MCH)

The MCH is calculated from the RBC and HGB, using the following equation:
HGB  g/dL 
MCH  pg  = ----------------------------------------x10
6
RBC(x10 /L)
(3) Mean cell hemoglobin concentration (MCHC)

The MCHC is calculated from the HCT and HGB, using the following equation:
HGB  g/dL 
MCHC  g/dL  = -------------------------------x100
HCT(%)

2.3.4 WBC Classification (CBC+DIFF mode)
White blood cells (leukocytes) can be broadly classified as lymphocytes, monocytes, or granulocytes.
Granulocytes can be further classified as neutrophils, basophils, or eosinophils, depending on the dye-
affinity of the granules. The applicable analysis procedure is explained here.
A 5DIFF analysis is used to identify and analyze the following white cell groups: lymphocytes,
monocytes, eosinophils, neutrophils, and basophils. The 5DIFF analysis procedure is explained here.
Figure 2-3-4: WBC/DIFF Analysis Procedure
(1) A fixed amount of 11µL of blood (55.5µL of diluted sample for capillary mode) is diluted by 1.0mL of
STROMATOLYSER-4DL in the WBC chamber.
At the same time, 30µL of STROMATOLYSER-4DS is added to stain the sample to a dilution ratio
of 1:94. (1:94 in capillary mode) After reacting for about 22 seconds, the red blood cells are hemo-
lyzed and the white blood cells are stained.
(2) The sheath syringe injects 95µL of diluted sample into the FCM flowcell.
(3) In the FCM flow cell, the sample is analyzed via flow cytometry method utilizing a semiconductor
laser.

2.3.5 WBC Classification (CBC mode)
Figure 2-3-5: WBC (CBC8) Analysis Procedure
(1) A fixed amount of 11µL of whole blood (55.5µL of diluted sample for capillary mode) is dispensed
into WBC chamber, diluted, and hemolysed with 1.0mL of STROMATOLYSER-4DL.

2.4 ANALYSIS PARAMETER
Table 2-4-1: Analysis Parameters (RBC, HGB)
Analysis Block RBC/PLT HGB

Principle Sheath Flow DC Detection Method Colorimetric
Analysis Channel RBC/PLT Channel HGB Channel
Information PLT Histogram RBC Histogram
Parameter PLT HCT RBC HGB
MCV,MCH,MCHC,
Analytical Parameter MPV,PDW, P-LCR RDW-SD/CV
Reagent CELLPACK SULFOLYSER
Table 2-4-2: Analysis Parameters (WBC)
Analysis Block WBC

Principle Flow Cytometry by using Semi-Conductor Laser
Analysis Channel WBC Channel 5DIFF Channel
Information WBC Histogram 5DIFF Scattergram
Parameter WBC# BASO% Lymph% MONO% NEUT% EOSI%
BASO# Lymph# MONO# NEUT# EOSI#
Reagent STROMATOLYSER-4DS STROMATOLYSER-4DS Lysing solution/ Staining Dye

2.5 REAGENT SYSTEM
Figure 2-5 Reaction Chamber Construction

2.6 ANALYSIS FLOW

2.7 FCM DETECTOR BLOCK
CAUTION:
Laser Diode is very sensitive to static electricity. Follow the
instruction below when you replace the Laser Diode.
- To prevent electrostatic discharge damage, touch the chassis to establish a
ground before servicing Optical Unit No.22.
- Remove jumper pin from the terminal on the Laser Diode
AFTER connecting to PCB No. 2168. If the jumper pin is removed before
connecting to PCB No. 2168, Laser Diode will be damaged.
- Do not disconnect the connector to the PCB No. 2168.
In the worst case, the device inside the Laser Diode will be destroyed.
- Before reapplying the power, be sure that VR-1 on PCB No. 2168 is turned
all the way CCW.
The life of the Laser Diode is approx. 10,000 hours.

2.7.1 Function
(1) Outline
Laser diode (Wave length: 633 nm) apply to samples in sheath flow generated flow cell. FCM
Detector Block detects front scattered light, side scattered light, and side fluorescence light from
these samples. XS generates two-dimensional scattergrams from these signals as follows.
X-axis Y-axis
WBC# SSCL FSCP
DIFF SSCL SFLP
SSCL: Side Scattered peak Logarithm

FSCL: Front Scattered peak Logarithm
SFLP: Side Fluorescence Light Peak linear
SFLL: Side Fluorescence Light peak Logarithm
FSCP: Front Scattered Peak linear
Following table shows which receiver is used for each parameter.

FSC Side Scattered Side Fluorescence
Photodiode Photodiode APD
WBC# / BASO Used Used Not Used
DIFF Used but not Used Used
displayed in
Scattergram
(2) Beam spot generating system

Beam spot generating system consists of laser diode, collimator lens. Laser beam generated by
laser diode is converted to parallel beam by collimator lens. This beam is collected by condenser
lens and applies to samples. Beam shape is as follows.
(3) Sheath flow system

Sheath flow is generated in the flow cell. Sample flows center of the flow cell.
(4) Front scattered light system
Front scattered light system consists of beam stopper and FSC condenser lens. Beam stopper
shade direct light from the laser diode. FSC condenser lens collect the scattered light of the sam-
ple.
(5) Dichroic mirror
Light below 640 nm wave length reflects to the side scattered light APD.
(6) Side fluorescence light system
Side fluorescence light system consists of interference filter, APD (Avalanche Photodiode). The
light through dichroic mirror is filtered by interference filter and fluorescence light over 660 nm
wave length is detected by APD. Detected fluorescence light signal is amplified by pre-amplifier
and transmitted to main amplifier.

(7) Side Scattered light system
The scattered light reflected by dichroic mirror is detected by photodiode. Detected scattered light
signal is amplified by pre-amplifier and transmitted to main amplifier.
(8) Cover switch
Cover switch is installed to prevent interference from the outer light and leakage of the laser beam.
If the cover is opened, XS stops laser radiation and a message is displayed.

2.8 OPTICAL SYSTEM

2.9 HYDRAULIC PARTS
2.9.1 Solenoid Valve Function

Following 2 types of direct operated solenoid valves are used in the XS hydraulic system.
2-way type: 3-way type:
2 each of 2-way solenoid valves are built-in a 2 each of 2-way solenoid valves are built-in a
module as shown below. Each S.V. operates module, and one port of each SV is connected
independently. as common port internally. The entire module
functions as a 3-way valve. Each SV has own
driver, therefore, the SV No. is assigned
separately. See the figure below.
Figure 2-10-1: 2-Way/3-Way SV Modules Used in XS Hydraulic System

Refer to the following table for the solenoid valve types.
Table 2-9-1: List of Solenoid Valves (in order of SV No.)
SV Function Located 2-way/ Diaphragm Ref.

No Unit 3-way Material Page
1 WB Asp Sensor Charge Valve No. 2-way EPDM 2-21
2 RBC/HGB Chamber Drain 182 Assy
3 Sheath Syringe Rinse Valve No. 3-way EPDM 2-21

4 RBC Back Flush 182 Assy
5 RBC Front Flush OUT Valve No. 3-way EPDM 2-21

6 RBC Sheath Flow 182 Assy
7 RBC Front Flush IN Valve No. 3-way EPDM 2-21

8 Rinse Cup Flush 182 Assy
9 EPK DP Replenish Valve No. 2-way EPDM 2-21

10 WBC/DIFF Chamber Drain 182 Assy
11 FCM Sheath Flow Valve No. 2-way EPDM 2-21

12 WBC/DIFF Sample Charge-1 182 Assy
13 Sample Charge DP Drain Valve No. 3-way EPDM 2-21

14 HGB Sample Charge 182 Assy
15 EPK Chamber Replenish Valve No. 2-way EPDM 2-21

16 RBC Sample Charge-1 182 Assy
17 EPK line air bubble removal Valve No. 2-way EPDM 2-21
18 Open EPK_DP discharge line to RBC/HGB 182 Assy
chamber
19 Waste chamber pressure line Valve No. 3-way EPDM 2-21
20 Waste chamber vacuum line 182 Assy
21 Air supply to CP needle (CP model only) Valve No. 2-way EPDM 2-21
22 Piercer needle internal rinse line open 182 Assy
23 DIL chamber pressure line (sheath pressure Valve No. 3-way EPDM 2-21
for RBC, DIFF analyses) 182 Assy
24 DIL chamber vacuum line (for diluent
replenish)
25 (reserved) Valve No. 2-way EPDM 2-21
26 (reserved) 182 Assy
27 FFD (DIFF lyse) DP dispense Hydraulic 3-way EPDM 2-20

28 FFD_DP replenish line open Unit-C Assy
29 Pressure supply to all DPs Hydraulic 3-way EPDM 2-20

30 Vacuum supply to all DPs Unit-C Assy
31 SLS (HGB lyse) DP dispense Hydraulic 3-way EPDM 2-20

32 SLS_DP replenish line open Unit-C Assy
33 DIFF sheath syringe switching FCM 2-way EPDM 2-20

34 DIFF charging line Reaction Unit
35 FFS (DIFF dye) DP dispense Hydraulic 3-way EPDM 2-20

36 FFS DP replenish Unit-B Assy
37 RBC sheath syringe switching RBC Valve 2-way EPDM 2-20

38 RBC charging line Assy

2.9.2 HYDRAULIC PARTS LOCATION
Figure 2-10-2-1: Left Side Interior
Figure 2-10-2-2: Front Interior
Figure 2-9-2-3: Right Top Interior

Figure 2-9-2-4: Hydraulic Unit-A Assy
Figure 2-9-2-5: Hydraulic Unit-B Assy
Figure 2-9-2-6: Hydraulic Unit-C Assy

(View from internal side)
XS Series S/M 2-19 July 6, 2009

Figure 2-9-2-7: Detector Block
Figure 2-9-2-8: Valve No. 182 Assy
Figure 2-9-2-9: Air Pump (No. 1 Assy)

Figure 2-9-2-10: Diluent Chamber Assy
Figure 2-9-2-11: CP Assy
Figure 2-9-2-12: Aspiration Unit Assy
Figure 2-9-2-13: Reaction Unit Assy

Figure 2-9-2-14: RBC Valve Assy

2.10 HYDRAULIC SEQUENCE DESCRIPTION [AD]
0.0 -1.5 sec.
[AD] 2016121

0-1.5 sec.
[Diaphragm Pump Control]
- Vacuum (-0.03MPa) is applied for pneumatic side of diaphragm pumps by activating
SV30 for 0.0 – 1.5 seconds.
- EPK diaphragm pump is replenished by activating SV9 for 0.0 – 1.5 seconds.
[RBC/HGB]
- Solution in RBC/HGB Chamber is drained to WC1 by activating SV2 for 0.0 – 1.5 sec-
onds.
[WB Aspiration Probe]
- The Whole blood syringe descends to aspirate 20µL of whole blood sample for 0.2 – 2.0
seconds.

1.5 – 2.5 sec.

1.5 – 2.5 sec..
- Pressure (+0.06MPa) is applied for pneumatic side of diaphragm pumps by activating
[RBC/HGB]
- EPK DP dispenses 1.0mL of diluent into RBC/HGB Chamber by activating SV18 for 1.5 –
2.5 seconds.

2.5 – 9.4 sec.

2.5 – 9.4 sec.
[RBC/HGB]
- EPK in RBC/HGB Chamber is charged into Hgb flow cell by activating SV14 for 8.5 – 9.0
seconds.
- Solution in RBC/HGB Chamber is drained by activating SV2 for 9.0 – 9.4 seconds.
[Probe Operation]
- Aspiration probe ascends to the up-most position through the rinse cup for 3.4 to 6.2 sec-
onds.
- EPK is pushed into Rinse cup by activating SV8 and PV-1 while the aspiration probe is
ascending through the cup for 3.4 – 6.4 seconds.

9.4 -10.1 sec.

9.4 -10.1 sec.
[RBC/HGB]
- EPK DP dispenses 1.0mL of EPK into RBC/HGB Chamber by activating SV18 for 9.4 –
10.1 seconds.

10.1 – 11.1sec.

10.1 – 11.1sec.
- FFD diaphragm pump is replenished by activating SV28 for 10.1 – 11.1 seconds.
- FFS diaphragm pump replenished by activating SV36 for 10.1 – 11.1 seconds.
[WBC-DIFF]
- Solution in WBC Chamber is drained by activating SV10 for 10.1 – 11.1 seconds.

11.1 – 12.0sec.

11.1 – 12.0sec.
[WBC-DIFF]
- FFD DP dispenses 0.5mL of FFD reagent into WBC Chamber by activating SV27 for 11.1
– 12.0 seconds.
[Sample Aspiration Check]
- Sample Charge Diaphragm Pump is drained by activating SV13 for 11.1 – 12.0 seconds.

12.0 – 14.2sec.

12.0 – 14.2sec.
- Vacuum (-0.03MPa) applied for pneumatic port of diaphragm pumps by activating SV30
for 12.0 – 14.2 seconds.
- FFD diaphragm pump is replenished by activating SV28 for 12.0 – 14.2 seconds.
- 1.0 mL of out of 2mL EPK in RBC chamber is charged into Sample Aspiration Sensor by
activating SV1 for “Short Sample” Monitoring, 13.2 – 13.8 seconds. (1.0 mL of EPK
remains in RBC Chamber after this operation)

14.2 – 15.5sec.

14.2 – 15.5 sec.
- Pressure “+0.06MPa” is applied for pneumatic side of diaphragm pumps by activating

15.5 – 17.0sec.

15.5 – 17.0 sec.
[HGB]
- 1.0 of EPK remained in RBC/HGB Chamber is charged into Hgb flow cell by activating
(For blank measurement)
[Probe Operation]
- Sample Aspiration probe at the sample aspirating position moves to above RBC Chamber
position driven by STM3 for 15.5 -16.9 seconds.
(At this time, initial position is confirmed beforehand)

17.0 – 18.0sec.

17.0 – 18.0sec.
- Pressure (+0.06MPa) is applied for pneumatic port of diaphragm pumps by activating
[Probe Operation]
- Sample aspiration probe descends into RBC/HGB chamber for 17.0 -18.1 seconds.
[RBC/HGB]
- RBC/HGB Chamber is drained by activating SV2 for 17.0 – 18.0 seconds.

18.0 – 20.0sec.

18.0 – 20.0 sec.
- EPK DP dispenses small amount of diluent into RBC/HGB Chamber as the as the primary
buffer to prepare whole blood being dispensed by activating SV18 for 18.0 – 18.1 sec-
onds.
- Sample aspiration probe dispense approx. 2.5µL of whole blood (top end discard portion)
into RBC Chamber swinging the probe itself slightly forward and backword for 19.0 -19.8
seconds.
- Simultaneously, EPK DP dispenses diluent into RBC/HGB chamber for 19.5 – 20.0 sec-
onds. (1mL in total)
At this time, whole blood previously dispensed is mixed with EPK by swirl effect when
EPK is injected into RBC chamber.

20.0 – 22.0 sec.

20.0 – 22.0 sec.
- Solution in RBC/HGB Chamber is charged into Blood Aspiration Sensor by activating SV1
for 21.0 - 21.7 seconds. (For Short Sample Check)
then
- Solution remained in RBC/HGB Chamber is drained by activating SV2 for 21.7 – 22.0
seconds.

22.0 – 23.0 sec.

22.0 – 23.0 sec.
SV29 for 22.0 - 23.0 seconds.
- Sample Charge Diaphragm Pump is drained by activating SV13 for 22.0 - 23.0 seconds.
[RBC/HGB]
- 1.0mL of EPK is dispensed into RBC/HGB Chamber by activating SV18 for 22.0 - 23.0
seconds.
[WBC-DIFF]
- To rinse the FCM measurement line and sheath nozzle, following two operations are pre-
formed.
1) FCM sheath flow is formed by activating SV11 for 22.5 - 24.0 seconds;
2) EPK is pushed into FCM measurement line by activation SV3, SV33 for 22.7 - 24.0
seconds, and SV 34 for 22.7 - 23.0 seconds.

23.0 – 24.0 sec.

23.0 – 24.0 sec.
- EPK Diaphragm Pump is replenished by activating SV9 for 23.0 – 25.2 seconds.
- FFD Diaphragm Pump is replenished by activating SV28 for 23.0 – 25.2 seconds.
[RBC/HGB]
seconds.
[WBC-DIFF]
- FCM Sample Charge line is rinsed by activating SV12 for 23.0 – 24.0 seconds.

24.0 – 25.3 sec.

24.0 – 25.3 sec.
[WBC-DIFF]
- WBC Chamber is drained by activating SV28 for 24.0 – 25.2 seconds.
[Others]
- EPK chamber is replenished by deactivating SV23, activating SV24 from 24.0 seconds
and SV15 from 24.5 seconds.
These SV’s (SV15, SV23, SV24,) state are kept until 37 seconds in maximum or 4.0 sec-
onds after EPK Chamber’s Float SW ON, and then turn to reveres status.

25.3 – 26.6 sec.

25.3 – 26.6 sec.
- Sample Charge Diaphragm Pump is drained by activating SV13 for 25.3 - 26.5 seconds.
[WBC-DIFF]
- FFD DP dispenses 0.5mL of FFD reagent into WBC Chamber by activating SV27 for 25.3
– 26.5 seconds.
[RBC/HGB]
- Aspiration probe in RBC/HGB chamber dispenses 4µL of RBC sample swinging the
probe itself slightly forward and backward by activating STM3 (CP F/B) for 26.7 – 27.1
seconds.
- 1.0mL of diluent is dispensed into RBC/HGB Chamber by activating SV18 for 25.3 – 26.5
seconds to dilute 4µL of whole blood sample.
(First dispense of EPK for RBC/HGB dilution.)
XS Series S/M 2-54 TB201243 May 2012

26.6 – 28.0 sec.

26.6 – 28.07 sec.
- EPK and FFD diaphragm pumps are replenished by activating SV9, SV28, and SV36 for
26.6 - 27.9 seconds.
[WBC-DIFF]
- WBC Chamber is drained two times by activating SV10 for 27.5 - 28.4 seconds, and 27.5
- 28.4 seconds respectively.

28.0 – 30.4 sec.

28.0 – 30.4 sec.
[WBC-DIFF]
- 0.5mL of FFD reagent is dispensed into WBC Chamber by activating SV27 for 28.5 - 29.4
seconds.
- 30µL of FFS reagent is dispensed into WBC Chamber by activating SV35 for 29.4 – 30.3
seconds.
(This operation is not performed in CBC mode.)
[RBC/HGB]
- 1mL of EPK is dispensed into RBC/HGB Chamber additionally by activating SV18 for
28.0 - 29.9 seconds.
At this time, 4µL of whole blood previously dispensed is mixed with 2mL of EPK by the
swirl effect when additional EPK is injected into RBC chamber.
[Probe Operation]
- Sample Aspiration Probe ascends from RBC/HGB chamber to up-most position for 28.7 –
29.8 seconds.
- Sample Aspiration Probe moves backward from RBC/HGB chamber to above WBC
chamber position for 28.7 – 29.8 seconds.
[Waste Chamber]
- Waste Chamber -1 is drained by activating SV 19 for 28.5 - 34.1 seconds.

30.4 – 32.9 sec.

30.4 – 32.9 sec.
for 30.4 - 32.9 seconds.
- EPK, FFD, FFS and SLS reagents are replenished into corresponding diaphragm pumps
respectively by activating SV9, SV28, SV32 and SV36 for 30.4 - 32.8 seconds.

32.9 – 34.7 sec.

32.9 – 34.7 sec.
- Sample Charge Diaphragm Pump drained by activating SV13 for 32.9 - 34.8 seconds.
[Probe Operation]
- Sample probe descends into WBC Chamber for 35.2 - 35.3 seconds.
[RBC/HGB]
- Sheath syringe injection path is switched to RBC/PLT measurement line by activating
SV37 for 32.7 - 57.3 seconds

34.7 – 37.5 sec.

34.7 – 37.5 sec.
[RBC/HGB]
- RBC sample is charged by activating SV16 and SV38 for 34.7 – 37.4 seconds.
[Probe Operation]
- Aspiration probe descends into WBC chamber for 35.2 –-36.3 seconds.
- Aspiration probe dispense 11µL of whole blood into WBC chamber swinging the probe
itself slightly forward and backward for 36.7 – 37.7 seconds.

37.5 – 38.8 sec.

37.5 – 38.8 sec.
[HGB]
- 0.5mL of SLS lyse is dispensed into RBC/HGB chamber by activating SV31 (and SV29)
for 37.6 – 38.7. At this time, 1mL of diluted RBC sample remained in the chamber is
mixed and hemolysed with SLS lyse by the swirl effect when SLS is injected into RBC
chamber.
(0.5mL of SLS lyse is added into remained 1mL of RBC/HGB diluted sample remained in
RBC/HGB chamber.)
[WBC-DIFF]
- 0.5mL of FFD reagent is dispensed into WBC chamber by activating SV27 and SV31 for
37.6 – 38.7 seconds. At this time, 11µL of whole blood previously dispensed is mixed with
1mL of FFD by the swirl effect when additional FFD is injected into the chamber.
This solution is maintained in the WBC Chamber for about 20 seconds for proceeding
reaction.
[RBC]
- RBC Detector is flushed by activating SV4, SV5 and SV7 for 38.3 - 39.1 seconds.

38.8 – 39.8 sec.

38.8 – 39.8 sec.
- FFD and SLS diaphragm pumps are replenished respectively by activating SV28 and
[RBC]
- RBC sheath flow is formed by activating SV6 for 39.1 – 58.0 seconds.
- Sheath Syringe ascends for 39.2 – 43.8 seconds at 750pps as RBC pre-drive.
[Probe Operation]
- Sample Aspiration Probe ascends from WBC chamber to upper end position for 38.4 –
39.5 seconds.

39.8 – 41.3 sec.

39.8 – 41.3 sec.
[Probe Operation]
- Sample Aspiration Probe moves forward to above RBC/HGB chamber for 39.8 – 40.9
seconds.

41.3 – 43.4 sec.

41.3 – 43.4 sec.
- SLS diaphragm pump is replenished by activating SV14 for 41.8 – 43.3 seconds.
[Probe Operation]
- Sample Aspiration probe descends into RBC Chamber for 42.8 – 43.7 seconds.

43.4 – 44.1 sec.

43.4 – 44.1 sec.
[RBC]
seconds.
- Sheath Syringe ascends for 43.9 – 55.7 seconds at 125pps as RBC measurement.

44.1 – 46.8 sec.

44.1 – 46.8 sec.
- Sample Charge Diaphragm Pump drained by activating SV13 for 43.4 – 46.8 seconds.
[RBC]
- Pressure (+0.06MPa) supply to EPK chamber is stopped to keep RBC sheath flow stable,
by deactivating SV23 for 44.2 – 55.8 seconds.
Meanwhile, only remained (charged) pneumatic pressure inside the EPK chamber is used
to form RBC sheath flow.
- Small amount of EPK is poured into RBC chamber by activating SV18 for 0.1 seconds
(44.2 – 44.3 seconds).
This EPK is poured as the buffer liquid for whole blood that dispensed in the next step.
- Remained EPK in EPK Diaphragm Pump is poured into RBC chamber via Sample Aspira-
tion Probe with the whole blood at the tail end discard portion in the probe by activating
By above two operations, approx. 2.5µL of whole blood is diluted with 1.0mL of EPK and this solu-
tion is used for “Sample not asp” check in the following sequence.

46.8 – 49.6 sec.

46.8 – 49.6 sec.
- Solution in RBC Chamber is charged into Sample Aspiration Sensor by activating SV1 for
47.8 – 48.6 seconds.
(For “Sample not Asp” error check)
- Solution in RBC Chamber is drained by activating SV2 for 49.3 – 49.6 seconds.

49.6 – 51.9 sec.

49.6 – 51.9 sec.
[RBC/HGB]
- 1.0mL of EPK is dispensed into RBC Chamber via Aspiration Probe by activating SV22
(Rinse Purpose / 1st dispense)

51.9 – 52.7 sec.

51.9 – 52.7 sec.

52.7 – 53.5 sec.

52.7 – 53.5 sec.
[RBC/HGB]
- 1.0mL of EPK is dispensed into RBC Chamber by activating SV18 for 52.7 – 53.5 sec-
onds.
(Rinse Purpose / 2nd dispense)

53.5 – 55.8 sec.

53.5 – 55.8 sec.
[Probe Operation]
- Sample Aspiration Probe ascends from RBC chamber to up-most position for 53.8 – 54.7
seconds.
- Sample Aspiration probe above RBC Chamber moves to above rinse cup for 55.0 – 55.8
seconds.
[RBC/HGB]
- RBC measurement operation finishes at 55.8 seconds.

55.8 – 57.4 sec.

55.8 – 57.4 sec.
- Sample Charge Diaphragm Pump drained by activating SV13 for 55.8 – 57.3 seconds.
[RBC]
- Some amount of EPK in RBC chamber is fed by sheath syringe (returning to home posi-
tion) into RBC charge line for rinse by activating SV38, 55.8 – 56.3 seconds
- Some amount of EPK is fed into the other side of RBC charge line by activating SV16 for
56.3 – 57.3 seconds.
- RBC Chamber is drained by activating SV2 for 55.7 – 56.2 seconds, and drained again
56.5 – 57.5 seconds.
[WBC-DIFF]
- Pressure (+0.06MPa) is re-applied to EPK Chamber by activating SV23 for 55.8 – 83 sec-
onds.
- Sheath syringe desceds to home position for 55.0 – 57.6 seconds to prepare following
DIFF measurement.
- FCM sheath flow is formed by activating SV11 for 55.8 – 81.4 seconds.

57.4 – 58.6 sec.

57.4 – 58.6 sec.
[WBC-DIFF]
- WBC-DIFF sample is charged by activating SV12 and SV34 for 57.4 – 58.6 seconds.

58.6 – 79.3 sec.

58.6 – 79.3 sec.
[WBC-DIFF]
- WBC-DIFF sample at FCM measurement line is injected into FCM Flow Cell by sheath
syringe for 58.6 – 60.9 seconds, and by activating SV38 for 58.6 – 79.3 seconds.
While in this period, drive speed of sheath syringe varies as follows;
(1) 58.6 – 60.9 seconds: 1114pps (FCM pre-drive)
(2) 61.0 – 79.3 seconds: 688pps (FCM measurement)

79.3 – 83.0 sec.

79.3 – 83.0 sec.
[Sheath Syringe Reset and Rinse]
- Sheath syringe descends for 9700 pulses, 79.3 – 83.0 seconds. For the space inside the
Sheath Syringe descending, EPK is fed by activating SV3 for 58.6 – 79.3 seconds.
- EPK is pushed into Sheath Syringe by activating SV33 and SV34 for 81.1 – 81.4 seconds.

83.0 – 100.5 sec.

83.0 – 100.5 sec.
From 83.0 seconds, instrument returns to READY status for the next analysis while aftertreatment
process is being continued.
When the next anaysis opreration trrigered by pressing [START] button, aftertreatment processs is
canceld, then the next analysis sequence will start.
NOTE:
Aftertreatment proccess includes reset operation of Sheath Syringe position. When aftertreatment is
canceled without resetting Sheath
Syringe position, position reset will be performed in the next anaysis sequence while SV33 is acti-
vated for 25.5 – 26.1 seconds before the next measurement process started.

- Sample Charge Diaphragm Pump drained by activating SV13 from 83.0 seconds.
- 1.0mL of EPK is dispensed into RBC Chamber by activating SV18 from 83.0 seconds.
[Others]
- EPK chamber is replenished by deactivating SV23, activating SV24 from 83.0 seconds
and SV15 from 83.5 seconds.
These SV’s (SV13, SV18, SV15, SV23, SV24,) state are kept until 100.5 seconds or until
4.0 seconds after EPK Chamber’s Float SW ON, and then turn to reveres status simulta-
neously.

100.5-103.2 sec.

100.5-103.2 sec.
- EPK Diaphragm pump is replenished by activating SV9 for 100.5 – 101.5 seconds.
[Rinse Operations]
- 1.0mL of EPK in RBC Chamber is charged into Hgb Flow Cell by activating SV14 for rinse
purpose, 100.5 – 101.5 seconds.
- EPK is pushed into FCM Flow Cell by activating SV11 for rinse purpose, 101.5 – 103.2
seconds.
- Sheath syringe descends for 5449 pulses, 101.5 – 103.4 seconds. For the space inside
the Sheath Syringe descending, EPK is fed by activating SV3 for 101.5 – 104.1 seconds.
- EPK is pushed into Sheath Syringe and WBC-DIFF measurement line for rinse purpose
by activating SV33, 101.5 – 104.1 seconds.
- EPK is pushed into WBC-DIFF sample charge line between WBC Chamber and mea-
surement line for rinse purpose by activating SV34, 102.5 – 103.2 seconds.
- Solution in Rinse Cup line is drained by making PV3 open from 103.2 seconds and this
status is kept until all operation completed.

103.2-104.8 sec.

103.2-104.8 sec.
SV29 for 103.2 – 104.8 seconds.
- Sample Charge Diaphragm Pump drained by activating SV13 from for 103.2 – 104.8 sec-
onds.
[Rinse Operations]
- 1.0mL of EPK is dispensed into RBC Chamber by activating SV18, 103.2 – 104.8 sec-
onds.
- Pinch Valve-3 at the Rinse Cup line is opened from 103.2 seconds and kept open until the
next analysis triggered to prevent the tube being stuck.
- Pinch Valve-1 and 2 at the Waste Chambers are also opened from 104.2 seconds and
kept open until the next analysis triggered to prevent the tubes being stuck.
- Pressure (+0.06MPa) is applied to drain WC1 by activating SV19 for 104.2 – 104.8 sec-
onds.

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SECTION 2 HYDRAULICS

2.1 PNEUMATIC SYSTEM........................................................................................................................1

2.2 USE OF PRESSURE AND VACUUM .................................................................................................2

2.3 HYDRAULIC SYSTEM ........................................................................................................................3

2.4 ANALYSIS PARAMETER ....................................................................................................................9

2.5 REAGENT SYSTEM .........................................................................................................................10

2.6 ANALYSIS FLOW..............................................................................................................................11

2.7 FCM DETECTOR BLOCK.................................................................................................................12

2.8 OPTICAL SYSTEM ...........................................................................................................................15

2.9 HYDRAULIC PARTS .........................................................................................................................16

2.10 HYDRAULIC SEQUENCE DESCRIPTION .....................................................................................23

XS Series S/M December 13, 2007

Figure2-3-0-1: XS Pneumatic System

XS Series S/M 2-1 September 2016

XS Series S/M 2-2 September 2016

Figure 2-3-0-1: Hydraulic System Flow in Whole Blood Mode

XS Series S/M 2-3 September 2016

Figure 2-3-0-2: Hydraulic System Flow in Capillary Mode

XS Series S/M 2-4 September 2016

Figure 2-3-1: RBC/HGB Analysis Procedure

XS Series S/M 2-5 September 2016

Figure 2-3-2: Hgb Analysis Procedure

2.3.3 Computing the Erythrocyte Indices

(1) Mean cell volume (MCV)

(2) Mean cell hemoglobin (MCH)

(3) Mean cell hemoglobin concentration (MCHC)

XS Series S/M 2-6 September 2016

Figure 2-3-4: WBC/DIFF Analysis Procedure

XS Series S/M 2-7 September 2016

Figure 2-3-5: WBC (CBC8) Analysis Procedure

XS Series S/M 2-8 September 2016

Analysis Block RBC/PLT HGB

Table 2-4-2: Analysis Parameters (WBC)

Analysis Block WBC

XS Series S/M 2-9 September 2016

Figure 2-5 Reaction Chamber Construction

XS Series S/M 2-10 September 2016

XS Series S/M 2-11 September 2016

The life of the Laser Diode is approx. 10,000 hours.

XS Series S/M 2-12 September 2016

SSCL: Side Scattered peak Logarithm

Following table shows which receiver is used for each parameter.

(2) Beam spot generating system

(3) Sheath flow system

XS Series S/M 2-13 September 2016

XS Series S/M 2-14 September 2016

XS Series S/M 2-15 September 2016

2.9.1 Solenoid Valve Function

Figure 2-10-1: 2-Way/3-Way SV Modules Used in XS Hydraulic System

XS Series S/M 2-16 September 2016

Table 2-9-1: List of Solenoid Valves (in order of SV No.)

SV Function Located 2-way/ Diaphragm Ref.

3 Sheath Syringe Rinse Valve No. 3-way EPDM 2-21

5 RBC Front Flush OUT Valve No. 3-way EPDM 2-21

7 RBC Front Flush IN Valve No. 3-way EPDM 2-21

9 EPK DP Replenish Valve No. 2-way EPDM 2-21

11 FCM Sheath Flow Valve No. 2-way EPDM 2-21

13 Sample Charge DP Drain Valve No. 3-way EPDM 2-21

15 EPK Chamber Replenish Valve No. 2-way EPDM 2-21