Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1

Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

QUESTION ONE:
Choose one correct answer and mark in the answer sheet:
(65×1=65 Marks, 65 min)
1. Which of the following is a polar amino acid?

a. b. c. d.
2. Branched chain amino acids are:
a. Cysteine and cystine
b. Tyrosine and Tryptophan
c. Glycine and Serine
d. Valine, Leucine and Isoleucine
3. An amino acid which contains a disulphide bond is:
a. Lysine c. Methionine
b. Homocysteine d. Cystine
4. Which among the following is a nutritionally essential amino acid for man?
a. Alanine c. Glycine
b. Proline d. Valine
5. The 21st amino acid present in human proteins is?
a. Tyrosine c. Glutamic
b. Proline d. Selenocysteine
6. The opposite protein structure indicates:
a. Antiparallel β- sheets
b. Chaperons
c. Parallel β - sheets
d. Helix structure

7. All the followings are modified amino acids EXCEPT:

a. γ-carboxyglutamate c. Pyrrolysine
b. 4-hydroxyproline d. 5-hydroxylysine
8. Non conservative changes in protein structures resulted in:
a. Alzheimer disease c. Prion disease
b. Sickle cell anemia d. Same biological activity of protein

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

9. All the followings are incorrect regarding β-sheet protein structures EXCEPT:
a. The amino acids are stabilized by covalent disulfide bonds
b. It is stabilized by intrachain H- bonds of amino acids every four residues.
c. It is stabilized by intrachain ionic bonds of hydrophobic residues.
d. Stabilized by interchain H- bonds between amino acids of two parallel
peptide chains.
10. Which of the following are examples of globular proteins?
a. Insulin and hemoglobin c. Hemoglobin and keratin
b. Collagen and hemoglobin d. Collagen and elastin
11. Which of the following statement about the peptide bond is true?
a. It is a carbon-carbon bond
b. It has cis hydrogen and oxygen groups
c. It is planar
d. It has rotational freedom
12. All of the following are correct regarding protein denaturation ECXEPT:
a. The denatured proteins are precipitated from solution
b. Extreme pH results in intramolecular electrostatic attraction which does
not change the conformational structure.
c. Urea and guanidinium chloride are denaturing agents
d. 2-mercaptoethanol and dithiothreitol are used to disrupt disulfide bonds
13. Which of the following amino acids is NOT required for dopamine synthesis?
a- Arginine b- Phenylalanine c- Tyrosine d-Both b & c

14. Sickle cell disease is characterized by all of the following EXCEPT:

a. HbS represents non-conservative substitution in primary structure.
b. It is a genetic disorder due to change in the α-globin gene.
c. The infant begins showing symptoms if HbF has been replaced by HbS
d. Sickle cell disease is severe in homozygotes.
15. Protein pool contraction means:
a. Protein synthesis equaling protein degradation
b. Decreased protein synthesis and increased degradation
c. Increased protein synthesis and decreased degradation
d. Regulation of antigen processing and apoptosis

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

16. Which of the following is correct regarding prion protein?

a. It is a causative agent of TSEs and Alzheimer disease
b. Noninfectious PrP consists of α-helices & β-sheets
c. Its insoluble fibrils are resistance to proteolytic degradation
d. The normal and the infectious forms are different in their primary structure
17. In conjugation step of UPP, which is correct?
a. Ubiquitin is attached to substrate protein between the C-terminal
methionine residue of ubiquitin and Lys residues of protein
b. It requires activating enzyme, conjugating enzyme, and ligase
c. The substrate protein is tagged by 26S proteasome
d. Ubiquitin conjugation is irreversible process
18. Bortezomib acts as:
a. Peroxidase inhibitor
b. Ligase inhibitor
c. Proteasome inhibitors
d. Trypsin inhibitor
19. Formation of serotonin from tryptophan requires the action of:
a. Dopa decarboxylation c. Peroxidase
b. Diamine oxidase d. Hydroxylase
20. Exopeptidases catalyse the hydrolysis of peptide bonds:
a. At amino terminal c. Of only dipeptides and tripeptides
b. At carboxyl terminal d. Both a and b
21. The amino acid which synthesizes many hormones is:
a. Alanine c. Phenylalanine
b. Valine d. Histidine
22. The amino acid which synthesizes carnitine is:
a. Valine c. Arginine
b. Lysine d. Histidine
23. HGPRTase enzyme catalyze the conversion of:
a. Guanine → GMP
b. Adenine →IMP
c. Hypoxanthine → IMP
d. Adenine → AMP

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

24. Which of the following enzymes is inhibited by Methotrexate?

a. HGPRTase c. DHF-reductase
b. Glutamine amidotransferase d. Transaminase
25. The committed step of purine biosynthetic pathway is catalyzed by:
a. HGPRTase c. DHF-reductase
b. Glutamine phosphoribosyl amidotransferase d. CPS-II
26. Pyrimidine and purine nucleoside biosynthesis share a common precursor:
a. PRPP c. Glycine
b. Fumarate d. Alanine
27. The first true pyrimidine ribonucleotide synthesized is:
a. UMP b. UDP c.TMP d. CTP
28. Which of the following is a tripeptide hormone, with the sequence of Glu-
His-Pro and released from hypothalamus?
a. ADH c. TRH
b. TSH d. Glutathione
29. Proline is synthesized from:
a. Alanine c. Glutamate
b. Glycine d. Asparagine
30. The enzyme which catalysis the following reaction is:

a. Asparginase c. ALT
b. AST d. Reductase

31. Serine can be formed from:

a. Arginine through transfer of a hydroxymethyl group
b. D-3-phosphoglycerate through reduction and then transamination
c. From NH4+ and CO2 by synthetase enzyme
d. Threonine through transamination

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

32. The antioxidant peptide in human body is:

a. Oxytocin c. Vasopressin
b. Glutathione d. Encephalin
33. Vasopressin hormone is:
a. Nanopeptide c. Tripeptide
b. Octapeptide d. Pentapeptide
34. All the following peptide hormones increase blood pressure EXCEPT:
a. Vasopressin c. Angiotensin
b. Aldosterone d. Serotonin
35. Met-encephalin amino acid sequence is:
a. —Gly—Arg—Lys—Phe—
b. —Arg—Lys—Phe—Asp—
c. —Tyr—Gly—Gly—Phe—Met—
d. —Tyr—Gly—Gly—Phe—Leu—
36. The illustrated structure of figure (K) is for……………….
a. Fructose c. Deoxyribose
b. Ribose d. Glucose (K)

37. The illustrated disaccharide (M) is ……………

a. Maltose c. Sucrose
b. Isomaltose d. Lactose
(M)

38. The nonbranching helical structure of starch is called …………………..

a. Amylose c. Dextrin
b. Amylopectin d. Amino sugars
39. The primer molecule for glycogen synthesis is called ………………………..
a. Glycosphingolipid c. Syndecan
b. Glycogenin d. Glypican
40. ……..…………….. consists of β-glucose units linked by β(1 → 4) bonds.
a. Cellulose c. Starch
b. Glycogen d. Glycosaminoglycan

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

41. The repeated units of disaccharide structure (W) is called ……….….……

a. Dermatan sulfate
b. Heparin
c. Chondroitin sulfate
(W)
d. Hyaluronic acid

42. …………………… is glycosaminoglycan present in horny structures formed from

dead cells like hair and nails.
a. Chondroitin sulfate c. Dermatan sulfate
b. Keratan sulfate d. Heparan sulfate
43. Thrombin and antithrombin are crystallized in the presence of a short
segment of …………………………..
a. Chondroitin sulfate c. Dermatan sulfate
b. Keratan sulfate d. Heparan sulfate
44. Which of the following statement about the carbohydrate digestion is
Correct?
a. Digestion of carbohydrates begins in stomach.
b. Salivary -amylase acts on starch breaking -(1→6) bonds.
c. Maltase cleaves maltose, producing glucose.
d. Lactase cleaves lactose producing galactose and fructose.
45. Which of the following statements about lactose intolerance is Correct?
a. It is due to lactose-deficient.
b. It can be treating by remove lactose from the diet.
c. Lactose is passed into the large intestine causing abdominal cramps,
diarrhea, and flatulence.
d. Both b and c are correct.
46. ….… are proteins conjugated to polysaccharides with serial repeat units
a. Proteoglycans c. Glycosphingolipids
b. Glycoproteins d. Collagen
47. …………………….. cuts proteins close to membrane surface to release syndecan
ectodomains outside the plasma membrane.
a. Protease c. Disaccharidase
b. Phospholipase d. Oligosaccharidase

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

48. Which of the following statement about glycoprotein is Correct?

a. It contains higher amount of protein than carbohydrate
b. It contains higher amount of carbohydrate than protein
c. Syndecan and glypican are examples of glycoprotein
d. Both b and c are correct
49. The protein structure (Q) is unique to……………………
a. Mucins
b. Transferrin
(Q)
c. Immunoglobulins
d. Collagen
50. …………………………. fits into the restricted spaces where the three chains of the
triple helix of collagen come together.
a. Glycine b. Proline c. Hydroxyproline d. Hydroxylysine
51. The reaction (S) is catalyzed by……………..
a. Lysyl oxidase
b. Prolyl oxidase (S)
c. Prolyl hydroxylase
d. Lysyl hydroxylase
52. The reaction (Z) is catalyzed by ………………
a. Lysyl hydroxylase
b. Lysyl oxidase
c. Vitamin C
(Z)
d. Vitamin D

53. The genetic inability to add the GlcNAc-GlcA disaccharide to the growing
heparan sulfate chain results in ………………...
a. Scheie syndrome c. Multiple hereditary exostoses
b. Hurler syndrome d. Scurvy
54. Maltotriose is considered ……………..
a. Monosaccharide c. Disaccharide
b. Oligosaccharide d. Polysaccharide

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

55. Which of the following is a ketohexose (ketone-containing hexose)?

a. Ribose b. Fructose c. Glucose d. Erythrulose
56. Which of the following is C4 epimer of glucose?
a. Mannose b. Fructose c. Galactose d. Sorbose
57. Which of the following statement about glypicans is correct?
a. They are membrane bounded glycoprotein
b. They are attached to the membrane by a lipid anchor
c. They contain higher amount of protein than carbohydrate
d. They are anchored to the membrane by peptide transmembrane domain
58. Glycogen is a more highly branched structure than amylopectin, with chains
of 12–14 monomers.
a. True b. False
59. Pancreatic -amylase acts on starch in small intestine breaking β-(1-4) bonds.
a. True b. False
60. Fructose is transported only by facilitated diffusion utilizing carriers that are
independent of Na+
a. True b. False
61. Heparan sulfate molecules could bind nonspecifically to extracellular proteins
and signaling molecules to alter their activities.
a. True b. False
62. Glycoconjugates consist of carbohydrates non-covalently bound with protein
or lipid.
a. True b. False
63. Shedding is highly regulated and is activated in proliferating cells, such as
cancer cells.
a. True b. False
64. The glycosaminoglycan in proteoglycan is joined to core protein through a
trisaccharide bridge.
a. True b. False
65. Glycosphingolipids are plasma membrane components and play a role in
signal transduction in cells.
a. True b. False

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

QUESTION TWO
Complete the following chemical equations with suitable substrates,
products and enzymes: (5 Marks, 10 min)
1- The compound name
is ………………….
2- The enzyme name is
……………………………
3- The cofactor name
is………………………………
4- The enzyme name
is……………………………..
5- The compound name
is………………………………

6- The enzyme name is

……………………………
7- The compound name
is ……………………………
8- The enzyme name is
………………………………
9- The enzyme name is
……………………………..
10- The compound name
is
………………………………
and DRAW its
chemical
structure…………….

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

QUESTION THREE:
I. Complete the following statements with correct terms:
(20x1.5=30 Marks, 20 min)
1- Regarding regulation of enzyme activity by feed-back inhibition, end product
may act as ……………………………………………………………. and ……………………………………………………………………………
2- Phosphorylation reactions are catalyzed by a family of enzymes called
…………………………..…………… and it's one of the method regulating enzyme activity by
…………………………………………..
3- During the enzyme-catalyzed reactions, enzymes provide an alternate reaction
pathway with ………………………………………………………..
4- The class of enzymes that catalyze the formation of bonds between carbon and
O, S, N atoms is named …………………………………………..………
5- …………………………………………..……… serve as recyclable shuttles such as folates
6- …………………………………………….. are closely related variants of the same enzyme with the
same catalytic function, but with different physical and chemical properties
such as ………………………………………………….
7- Sildenafil promotes penile erection by competing with the
…………………………………………………………. leading to the inhibition of
…………………………………………………..………. enzyme
8- The neurotoxic effects of organophosphorus insecticides are a result of
…………………………………………………………………..…
9- Certain proteins such as proteases are synthesized and secreted as inactive
precursor known as ………………………………………………………….
10- Effectors induce a ………………….……………………….. in enzyme, altering it's
…………………………………..…………… for a substrate

11- The number of molecules of substrate converted to product per enzyme

molecule per second is called ………………………………………………….
12- The inducible enzymes are defined as ………………………………………………………………………..……………
13- Regarding Lineweaver-Burk plot, competitive enzyme inhibitors increase
……………………………….…… so the affinity of the enzyme to its substrate is decreased

14- In the lock and key model of enzyme action the part of the enzyme that
recognizes the substrate is known …………………………………………………..……….
15- If an enzyme solution is saturated, the most effective way to obtain an even
faster yield of products would be ………………………………………………

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

II. Given the following Eo, calculate the ∆ G0- of oxidation of FADH2 by O2
(½ O2 + FADH2 → H2O + FAD): (2.5 Marks, 5 min)
½ O2 + 2 H+ + 2 e- → 2H2O Eo = +0.820 V
FAD + 2 H+ → FADH2 Eo = -0.219 V
Remember: ∆ G0- = - nF∆ Eo and F=23.06
..........................................................................................................................................
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..........................................................................................................................................

III. The following figure illustrates the reactions of electron transport chain
and oxidative phosphorylation, complete the following as indicated:
(7x2.5=17.5 Marks, 10 min)

1. Explain why CoQ is the suitable interface between two-electron carriers and the
one-electron carriers.
............................................................................................................................. ........................................................................

.....................................................................................................................................................................................................

.............................................................................................. ......................................................................................................

2. What is the hypothesis that explains how the free energy generated by the
transport of electrons by ETC is used to produce ATP?
............................................................................................................................. ........................................................................

.....................................................................................................................................................................................................

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

3. In resting conditions, the demand ATP synthesis is limited. Is PMF high?

Yes No
4. What is the P/O ratio of succinate?
.............................................................................................................. ...............................................................................

................................................................................................................................................................................. ...........

5. Illustrate the biological importance of thermogenin.

............................................................................................................................. ................................................................

.............................................................................................................................................................................................

6. What are the inhibitors of complex IV of ETC?

.............................................................................................................................................................................................

.............................................................................................................................................................................................

7. What is the dominant shuttle for aerobic oxidation of cytosolic NADH in heart and
muscle tissues?
......................................................................................................................... ....................................................................

....................................................................................................................................................................................... ......

QUESTION FOUR:

I. Answer each of the following questions as required:

(3x4= 12 Marks, 15min)
1. Describe with structural chemical equation the rate limiting step of Heme
biosynthesis

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

2. Some fatty acids are essential in human and must be provided in diet (give
reasons and name of two examples)

3. Define the four major groups of plasma lipoproteins and give the function of
each group

II. Compare between each pair of the following: (3X6=18Marks, 15min)

1- Carbon-Monoxide poisoning and methemoglobinemia
Items Carbon Monoxide Poisoning Methemoglobinemia

Definition

Symptoms

Treatment

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Department of Biochemistry, Faculty of Pharmacy, Tanta University, Final Biochemistry -1
Exam (3043), First Semester, Second-Year Pharmacy Students, 12th January/2020

2- Multiple sclerosis and respiratory distress syndrome

Items Multiple sclerosis Respiratory distress

syndrome

Genetic cause

Symptoms

3- Triacylglycerol and glycerophospholipids

Items Triacylglycerol Glycerophospholipid

Chemical
structure

Digestive
pancreatic
enzyme

Structural
chemical
equation that
describe
pancreatic
digestion

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