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    BMS533 Practical 2

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    Allisya Nasir
    This document provides guidelines for good microbiological practices (GMPs) for routine work in a BSL2 laboratory. It outlines proper procedures for before, during, and after entering the laboratory, including wearing proper protective equipment, maintaining clean work areas, following safety protocols, and properly storing materials. The guidelines are intended to establish standard practices to promote safety for all laboratory personnel handling microbes that can cause infection. A rubric is also included for evaluating GMP guideline documents.

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    © All Rights Reserved
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    BMS533 Practical 2

    Uploaded by

    Allisya Nasir
    123 views5 pages
    This document provides guidelines for good microbiological practices (GMPs) for routine work in a BSL2 laboratory. It outlines proper procedures for before, during, and after entering the laboratory, including wearing proper protective equipment, maintaining clean work areas, following safety protocols, and properly storing materials. The guidelines are intended to establish standard practices to promote safety for all laboratory personnel handling microbes that can cause infection. A rubric is also included for evaluating GMP guideline documents.

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    This document provides guidelines for good microbiological practices (GMPs) for routine work in a BSL2 laboratory. It outlines proper procedures for before, during, and after entering the laboratory, including wearing proper protective equipment, maintaining clean work areas, following safety protocols, and properly storing materials. The guidelines are intended to establish standard practices to promote safety for all laboratory personnel handling microbes that can cause infection. A rubric is also included for evaluating GMP guideline documents.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    123 views5 pages

    BMS533 Practical 2

    Uploaded by

    Allisya Nasir
    This document provides guidelines for good microbiological practices (GMPs) for routine work in a BSL2 laboratory. It outlines proper procedures for before, during, and after entering the laboratory, including wearing proper protective equipment, maintaining clean work areas, following safety protocols, and properly storing materials. The guidelines are intended to establish standard practices to promote safety for all laboratory personnel handling microbes that can cause infection. A rubric is also included for evaluating GMP guideline documents.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 5

    BMS533

    Biorisk Management

    GROUP : AS201 2B1 TEAM : 1

    Practical 2 : Good Microbiological Practise

    LEADER :
    Members :
    :
    :

    Date : 11 APRIL 2022

    Report writing :

    This section was written by and reviewed by


    Introduction :

    Methods :

    Results :

    Discussion :
    BMS533 PRAC# 2 : Good Microbiological Practices (GMP)

    Objective

    To write set of GMPs for routine work in a BSL2 laboratory

    Introduction
    GMPs are central to any biorisk management programs. Most of you should have been taught
    the basic GMP when you are first introduced to the Microbiology Lab. For this session, we will
    attempt to write a set of GMP guidelines for routine work in a BSL2 laboratory, where microbes
    that can cause infections are routinely handled. The purpose is for everyone to be on the same
    page, as different lecturers may have taught you slightly different versions of GMPs. We will
    do this by considering the 'correct' actions to take from the moment you want to enter the lab
    to the point you leave the lab.

    GOOD MICROBIOLOGICAL PRACTICES


    Applicable for a BSL2 laboratory and handling of Risk Group 2 organisms and below.

    A. BEFORE YOU ENTER THE LAB


    a. DO
    1. Leave all personnel belongings outside for instance bags and jackets.
    2. Other things such as watches and jewellery must be taken off before entering the lab.
    3. Wear closed-toed shoes and long pants to prevent the skin from exposure to the chemicals.

    b. DO NOT
    1. Do not bring in your mobile phone.
    2. Do not wear loose sleeves, open-toed shoes, and contact lenses as they are impractical
    when working.
    3. Do not bring the food or drinks into the lab.

    B. UPON ENTERING THE LAB


    a. DO
    1. Put on a lab coat and it should be worn with the sleeves rolled down.
    2. Wear gloves and safety goggles to prevent the skin and eyes from exposure to the
    chemicals.
    3. Long hair needs to be tied up to prevent the hair from being burned while working with a
    Bunsen burner.
    b. DO NOT
    1. Do not leave a long hair untied.
    2. Not wearing lab coat, safety goggles, and gloves.

    C. WORKING IN THE LAB


    a. DO
    1. Keep the workspace free of all unnecessary materials.
    2. Disinfect work areas before use.
    3. Read the precautions in the laboratory manual and follow the instructions exactly.
    4. Handle the chemicals carefully and follow the warnings.
    5. Handle hot glassware and apparatus carefully as hot glassware looks the same as cold
    glassware.
    6. Use glove when handling hazardous chemicals.
    7. Label the test tubes clearly.
    8. Report any spills, injuries, or accidents to the instructor immediately.
    9. Clean up any broken glassware immediately and carefully and dispose the broken glassware
    in the bin labelled ‘FOR BROKEN GLASS ONLY’.
    10. Turn off the heating apparatus, gas valves and water faucets when not in use.
    11. Wash hands immediately after in contact with allergens, pathogenic organism, or toxic
    chemicals.

    b. DO NOT
    1. Do not distract and obstruct lab mates while conducting experiment.
    2. Do not play with material and apparatus in the lab.
    3. Do not touch, smell or do anything before you know what the chemical is.
    4. Do not conduct an experiment without the assistance of a laboratory assistant.
    5. Do not run in the laboratory.
    6. Do not leave the experiment while in progress.
    7. Do not eat or drink while working in the laboratory.
    8. Do not sit on the laboratory benches.
    9. Do not leave the lit Bunsen burner unattended.
    10. Do not fill a pipette using mouth suction.
    11. Do not light a flame near flammable solvents.
    12. Do not intentionally smell or taste chemicals.
    13. Do not apply any cosmetics while working in the lab.

    D. CLEANING UP AFTER YOU ARE DONE


    a. DO
    1.
    2.

    b. DO NOT
    1.
    2.

    E. UPON EXITING THE LAB


    a. DO
    1. Remove all laboratory protective clothing and safety gear like lab coats, gloves, and safety
    goggle before leaving.
    2. Switch off the lights and ventilation fan.
    3. Place all materials and apparatus properly according to the original place arrangement.
    4. Sign the laboratory record register book to record the attendance of time leaving.

    b. DO NOT
    1. Do not rush to exit the laboratory.
    2. Do not leave the laboratory untidy.
    3. Do not remove or bring out any equipment or chemicals from the laboratory.
    4. Do not block the exit.

    REFERENCES

    Carolina, C. (2021, December 3). Lab Safety Dos and Don’ts for Students. Carolina Knowledge Center.
    Retrieved April 20, 2022, from https://knowledge.carolina.com/professional-
    growth/safety/lab-safety-dos-and-donts-for-students/

    Choudhary, A. (2009). SOP for Guidelines for Working in Microbiology Laboratory. Pharmaceutical
    Guidelines. Retrieved April 20, 2022, from https://www.pharmaguideline.com/2009/01/sop-
    for-guidelines-for-microbiology.html

    James, D. E. (2022). Nine Safe Practices for the Microbiology Laboratory. Carolina.Com. Retrieved
    April 20, 2022, from https://www.carolina.com/resources/detail.jsp?trId=tr11085

    Manager, L. (2021, December 22). Lab Safety Rules and Guidelines. Lab Manager. Retrieved April 20,
    2022, from https://www.labmanager.com/lab-health-and-safety/science-laboratory-safety-
    rules-guidelines-5727
    General Laboratory SOP # 1016
    Faculty of Applied Sciences 2021

    Lab Report Rubric PRAC # 2 (include this with your set of guidelines)

    Exceeds Below
    Attribute Meet Expectations Marks
    Expectations Expectations
    Marks 5 3 1
    1. Punctuality Submitted early Submitted on time Submitted late
    Was the report submitted on
    time?
    2. Organization of Guidelines Fully adhere to format Mostly adhere to Not organized
    Is the prescribed format used? format

    3. Originality of Guidelines Original, with creative Mostly written in own More than 50% of
    Is the report original, or are insights words, attempts to report was
    most parts duplicated from the summarise duplicated
    manual? verbatim
    4. How comprehensive is Full details for easy Details included, but Not enough
    section compliance still some ambiguity details
    “A. Before you enter the Lab”

    5. How comprehensive is Full details for easy Details included, but Not enough
    section compliance still some ambiguity details
    “B. Upon entering the Lab”

    6. How comprehensive is Full details for easy Details included, but Not enough
    section compliance still some ambiguity details
    “C. Working in the Lab”

    7. How comprehensive are Full details for easy Details included, but Not enough
    sections compliance still some ambiguity details
    D & E.

    8. Is the language clear Clear and easy to Clear, but still a bit of Not clear.
    follow gray areas

    9. Grammars, spellings Few, less than 3 Three to 10 mistakes More than 10


    (per page) mistakes mistakes
    10. References Used proper software Manually with No or inadequate
    Are references correctly cited? mistakes citation
    SUM (50)
    Bonus marks for nice design, attractive look, professional feel (10)
    TOTAL MARKS (60)

    Evaluated by : Date :

    Biosafety Committee
    Faculty of Applied Sciences, UiTM Shah Alam
    Tel. 0185122516 email : [email protected]

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