CFX96 Touch™ Real-Time PCR Detection System

Specifications

Real-Time PCR Bulletin 6075

Advancing qPCR Together

The CFX96 Touch Real-Time PCR Detection System lets you quickly set up runs, easily
monitor their progress, and use powerful analysis tools. Solid-state optical components
provide sensitive detection for precise quantification and target discrimination. Five-target
multiplexing enables powerful simultaneous analyses. Tailor the run to detect SYBR®
Green in the single-color fast scan mode. Set up runs and view data traces in real time on
the integrated touch screen. CFX Maestro™ Software is customizable for all levels of users
and different experiment needs. A startup wizard and intuitive experiment setup make
it easy to get started with real-time PCR. Extract meaningful information from runs and
visualize data using bar charts, box-and-whisker plots, dot plots, clustergrams, scatter
plots, and volcano plots. Further analyze data using statistics, including t-tests and
one-way ANOVA.

Specifications
C1000 Touch™ Thermal Cycler with 96-Well Reaction Module
Maximum ramp rate 5°C/sec Temperature range 0–100°C
Average ramp rate 3.3°C/sec Temperature accuracy ±0.2°C of programmed target at 90°C
Heating and cooling method Peltier Temperature uniformity ±0.4°C well-to-well within 10 sec of
Lid Heats up to 105°C arrival at 90°C
Gradient
Operational range 30 –100°C
Programmable span 1– 24°C
Optical Detection
Excitation 6 filtered LEDs Dynamic range 10 orders of magnitude
Detection 6 filtered photodiodes Scan time
Range of excitation/ 450–730 nm All channels 12 sec
emission wavelengths Single-channel fast scan 3 sec
Sensitivity  etects 1 copy of target sequence in human
D Multiplex analysis Up to 5 targets per well
genomic DNA
Software
Operating systems Windows 7, 8, and 10, Mac OS X El Capitan and Data export Save, copy, and print all
OS Sierra (Mac version for data analysis only; graphs and spreadsheets
not for instrument control) from right-click menu
Memory Minimum of 1 GB Export specified data in
Data analysis modes PCR quantification with standard curve multiple formats
Melt curve analysis Copy and paste into Microsoft
Excel, Word, or PowerPoint file
Gene expression analysis by relative quantity (∆Cq) or
normalized expression (∆∆Cq) Customizable reports containing
run settings, data graphs, and
Data analysis options include bar charts,
spreadsheets can be directly
box-and-whisker plots, dot plots, clustergrams,
printed or saved as PDFs
scatter plots, and volcano plots
Image Export Export images at any pixel size and
Statistics, including t-tests and ANOVA
at resolution up to 600 dpi. Save
Multiple-file gene expression analysis for comparison images as bmp, jpg, or png files
of an unlimited number of Cq values
Allelic discrimination
End-point analysis
System
Licensed for real-time PCR Yes Electrical approvals IEC, CE
Sample capacity 96 wells Dimensions (W x D x H) 33 x 46 x 36 cm (13 x 18 x 14 in.)
Sample size 1–50 µl (10–25 µl recommended) Weight 21 kg (47 lb)
Communications USB 2.0 or above
CFX96 Touch™ Real-Time PCR Detection System

A B Catalog # Description
104
10 4 1855195  CFX96 Touch Real-Time PCR Detection System, includes
C1000 Touch Thermal Cycler Chassis, CFX96 Optical Reaction
RFU

103 103 Module, communication cable

12004110 CFX Maestro Software
102 102 12004128 CFX Maestro Software for Mac
0 10 20 30 40 0 10 20 30 40 12005258 CFX Maestro Software, Security Edition, includes 1 user
Cycles Cycles
license, installation CD, HASP HL key
C D 1845025 Precision Melt Analysis™ Software, includes 2 user licenses,
104 104 installation CD, 2 HASP HL keys, melt calibration kit
1845075  CFX Automation System II, includes plate handler and barcode
RFU

103 103 scanner, mounting plate, automation software

1814000 PX1™ PCR Plate Sealer, includes heat sealing instrument
102 102 1814030 Optically Clear Heat Seal, for use with PX1 PCR Plate Sealer, 100
0 10 20 30 40 0 10 20 30 40 MSB1001 Microseal® 'B' Adhesive Seals, optically clear, 100
Cycles Cycles HSP9655 Hard-Shell® Low-Profile 96-Well Skirted PCR Plates,
E F white well, white shell, 50
104 35 HSP9955 Hard-Shell Low-Profile 96-Well Skirted PCR Plates,
30 white well, white shell, barcoded, 50
25 1708840 iScript™ Reverse Transcription Supermix for RT-qPCR,
RFU

Cq

103 20
25 x 20 μl reactions, includes 100 μl 5x iScript RT Supermix,
15
10 iScript RT Supermix No-RT Control
102
0 10 20 30 40 2 3 4 5 6 7 8 1725037 iScript Advanced cDNA Synthesis Kit for RT-qPCR,
Cycles log starting quantity 25 x 20 μl reactions, includes 100 μl 5x iScript Advanced
Reaction Mix, 25 μl iScript Advanced Reverse Transcriptase
Linearity of five-target multiplex detection. A–E, fluorescence data from
1725848  iQ™ Multiplex Powermix, 50 x 50 μl reactions, 2x mix contains
a series of tenfold dilutions of plasmid DNA (108 –102 copies) amplified using
dNTPs, 11 mM MgCl2, iTaq™ DNA Polymerase, stabilizers
reporter dyes to monitor five targets: FAM/actin ( n ); HEX/GAPDH ( n );
1725270  SsoAdvanced™ Universal SYBR® Green Supermix,
Texas Red/cyclophilin ( n ); Cy5/tubulin ( n ); Quasar 705/IL-1b ( n ); F, standard
curves generated from data in A–E, reaction efficiencies range from 97 to 103%. 2 ml (2 x 1 ml vials), 200 x 20 µl reactions, 2x qPCR mix,
Cq, quantification cycle; RFU, relative fluorescence units. contains Sso7d fusion polymerase, ROX Normalization Dyes
1725280 SsoAdvanced Universal Probes Supermix, 2 ml (2 x 1 ml vials),
104 200 x 20 µl reactions, 2x qPCR mix, contains Sso7d fusion
polymerase, ROX Normalization Dyes
103 1725160  SsoAdvanced PreAmp Supermix, 1.25 ml (1 x 1.25 ml vial),
50 x 50 µl reactions, 2x PreAmp Mix, contains dNTPs,
RFU

Sso7d fusion polymerase, salts, enhancers, stabilizers, other

102
proprietary components
1725095  SingleShot™ SYBR® Green One-Step Kit, 100 x 50 µl reactions
0 10 20 30 40
Cycles Visit bio-rad.com/web/CFX96TouchSpecs for more information.
Exceptional reproducibility can be achieved with SsoFast™ EvaGreen®
Supermix. Efficient discrimination and reliable quantification can be obtained Cy is a trademark of GE Healthcare group companies. EvaGreen is a trademark
from 1.33-fold serial dilutions of input template. The CBP gene was amplified of Biotium, Inc. Bio-Rad Laboratories, Inc. is licensed by Biotium, Inc. to sell
from varying amounts of human genomic DNA (5 ng–500 pg). From left to reagents containing EvaGreen Dye for use in real-time PCR, for research
right: 5 ng, 2.83 ng, 1.60 ng, 903 pg, and 511 pg (n); 3.76 ng, 2.13 ng, purposes only. Excel, Microsoft, PowerPoint, and Windows are trademarks of
1.20 ng, and 679 pg (n). CBP efficiency = 96.5%, r = 0.996. Inset is a magnified Microsoft Corporation. Mac OS and OSX are trademarks of Apple Inc. HASP
view showing robust discrimination and reproducible amplification.
is a trademark of Aladdin Knowledge Systems, Ltd. Quasar is a trademark of
RFU, relative fluorescence units.
Biosearch Technologies, Inc. FAM, ROX, SYBR, and Texas Red are trademarks
of Life Technologies Corporation. Bio-Rad Laboratories, Inc. is licensed by Life
Ordering Information Technologies Corporation to sell reagents containing SYBR Green I for use in
Catalog # Description real-time PCR, for research purposes only.

1841100  1000 Touch Thermal Cycler Chassis, includes USB flash

C Bio-Rad’s real-time thermal cyclers are covered by one or more of the following
drive, power cord; does not include reaction module U.S. patents or their foreign counterparts owned by Eppendorf AG: U.S. Patent
CFX96™ Optical Reaction Module, for use with C1000 Touch
1845097  Numbers 6,767,512 and 7,074,367.
Thermal Cycler Chassis, includes communication cable Hard-Shell Plates are covered by one or more of the following U.S. patents
1855196 
CFX96 Touch Real-Time PCR Detection System with Starter or their foreign counterparts owned by Eppendorf AG: U.S. Patent Numbers
Package, includes C1000 Touch Thermal Cycler Chassis, 7,347,977; 6,340,589; and 6,528,302.
CFX96 Optical Reaction Module, CFX Maestro Software,
license for qbase+ Software, communication cable,
reagents, consumables

Bio-Rad
Laboratories, Inc.

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Bulletin 6075 Ver D US/EG 17-0999 1017 Sig 1216