OLYMPUS

M514 M516
AU400

Set Up Manual

Rev. 0300

Service Group
Mishima Olympus Co. Ltd.

Revision Date Revision Date

Issued 1998.06.05
0100 2000.04.30
0200 2000.09.05
0300 2002.01.31

Cover page ST_CV_1.DOC

AU400 Set Up MANUAL HISTORY TABLE

P. 1
REV. PAGE REPLACE HISTORY
0100 Cover Yes Addition (Rev. 0100)
0200 Cover Yes Addition (Rev. 0200)
0-1 Yes Addition (4-9, 7-7)
1-5 Yes Addition (ME6332 Tray)
1-6 Yes Addition (Name plate)
4-9 Yes Addition
7-1 Yes Revise
7-2 Yes Revise (Page No)
7-3 Yes Revise (Page No)
7-4 Yes Revise (Page No)
7-5 Yes Revise (Page No)
7-6 Yes Revise (Page No)
7-7 Yes Revise (Page No)
0300 1-1 Yes Addition of attention matter
M514 SET UP MANUAL
TABLE OF CONTENTS
0-1
0200

1. Set up Preparation.......................................................................... 1-1 to 1-6

2. Set up Flow ..................................................................................... 2-1

3. Unpacking....................................................................................... 3-1 to 3-4

4. Set up Procedure ............................................................................ 4-1 to 4-9

5. Set up personal computer .............................................................. 5-1

6. Processing the heater line of JD.................................................... 6-1

7. Operation Check............................................................................. 7-1 to 7-7

8. Test running.................................................................................... 8-1 to 8-2

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M514 SET UP MANUAL
1-1
Set up preparation 0300

n Installation Environment Conditions

l Installation Environment Conditions

• Site not subject to direct sunlight
• Site not minimum amount of dust
• Site that is flat (gradient : less than 1/200)
• Site with minimum vibration
• Site with floor construction that can support approximately 480Kg (4902N)
• Places located less than 2000m above sea level

l Power and noise Conditions

• Site which be located so that 10m water supply ad drain hoses
• Site with a power source with minimum voltage fluctuation (+-10%)
• Site the switchboard which is located so that a power cable with a length of 10m will reach the system.
• A place with no institution sending the signal of vertical polarization of 280 to 300 MHz to the
neighborhood (The police, a fire department, and public institution may be equivalent)
• A place with no corrosive gas.

l Temperature and humidity conditions

• The temperature of the installation room should be between 18 and 32°C, and the temperature fluctuation
during analysis should be +-2°C. (the calorific value of the environments : approx. 2600Kcal/H)
• The humidity must be between 40 and 80%RH with no condensation. (Humidity condition for
installation : between 5 and 40 °C)

l Water Supply and Drainage conditions

• An aqueduct gate must be within 10 m of the water supply gate of the instrument and the water waste gate
must be under 0.1 m in height. (If the optional forced waste water unit is used, a height of 1.5 m is
required.)
• Inner diameter of water waste gate must be greater than 48 mm.
• The purity of DI water must be under a conductivity of 2.0 us/cm. (The CAP type 2 level.)
• The temperature must be greater than 5 °C.
• The pressure in the aqueduct must be between 49 and 392 KPa. (0.5 to 4 kgf/cm) (Caution ! : If the water
pressure is greater than 2 kgf, attach a water supply unit.)
• Average amount of water required : 22.8 L/h (50/ 60Hz)
• Maximum instantaneous amount of water required : 0.7 L/min. (50/ 60Hz)
• Kind of waste liquid
Concentrated waste liquid(sample + reagent) 1.3 L / H (including reagent)
Washing waste liquid 25 L / H

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M514 SET UP MANUAL
1-2
Set up preparation

n Space requirements
l As shown in the figure below, this system requires space around it for safe installation and maintenance.

l Dimensions of system main units (Unit:mm)

Analyzer Width 1450 x Depth 760 x Height 1210
DPR Width 700 x Depth 700 x Height 1430

l Power supply
Voltage
USA 110V
Europe 230V
Australia 240V
Asia 110V or 220V
Japan AC100 or 200V

Mono phase 50/60 Hz+-1Hz

Voltage change Within +-10%

Electric power
3.5KVA
Switchboard
The switchboard should be located so that the standard power supply cable(10 m) will reach the instrument.

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M514 SET UP MANUAL
1-3
Set up preparation

n Options
There are specific optional device models depending on where the instrument will be used.

No. Name Jap USA Euro Asia Aust Contents

an pe ralia
MU4970 M514-OPA01 (1) ISE unit
MU4971 M514-OPA02 (1) ISE unit
MU4972 M514-OPA03 (1) ISE unit
MU4986 M514-OPA04 (1) ISE unit
MU4702 M520-OPH02 (2) Forced waste water unit (for CSA)
MU4148 M520-OPH01 2 Forced waste water unit (for LVD)
MU3351 M240-OPH03 (2) Forced waste water unit
MU2535 M240-OPD01 (1) (1) (1) Water supply unit (for CSA)
MU0815 M250-OPE01 (1) Water supply unit
MU4146 M520-OPE01 (1) Water supply unit (for CSA)
MU4147 M520-OPE02 (1) Water supply unit (for IEC)
MU1853 M240-OPH01 (1) (1) Forced waste water unit

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M514 SET UP MANUAL
1-4
Set up preparation

n Checking the contents of the package

Name Japan USA Europe Asia Australia
ANL main body 1 1 1 1 1
Cable set 1
PC rack 1
Floor plate 4 4 4 4 4
Standard accessories 1 1 1 1 1
Starting kit 1
Package type inner 1 1 1 1 1
outer 1 1 1 1
(Special accessories)

* For information about the main configuration, refer to the Function and Mechanism manual.

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M514 SET UP MANUAL
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Set up preparation 0200

n Standard accessories list

No. Name Japan USA Europe Asia Australia
MU8092 NE rack W 1 1 1 1 1 φ 11.5 to φ16.0 10
pcs/set
MU8093 NE rack B 1 1 1 1 1 φ 11.5 to φ16.0
MU8094 NE rack G 1 1 1 1 1 φ 11.5 to φ16.0
MU8095 NE rack R 1 1 1 1 1 φ 11.5 to φ16.0
MU8096 NE rack Y 1 1 1 1 1 φ 11.5 to φ16.0
MU8097 NE rack O 1 1 1 1 1 φ 11.5 to φ16.0

MV2313 PROGRAM 1
MV2314 PROGRAM 1
MV2315 PROGRAM 1
MV2316 PROGRAM 1 1

MV1764 Communicatio 1 1 1 1 1
n cable
MU8108 Template 1
DW4114 Code set 1 125V, 3A, 3m
ZM0634 Cuvette 1 1 1 1 1 10 pcs/ set
MU9934 S probe 1 1 1 1 1
MU9958 R probe 1 1 1 1 1
MU9888 Luminous 1 1 1 1 1
lamp
ME3333 Partition 20 20 20 20 20 For 30 ml bottle inside
ME4610 Partition 20 20 20 20 20 For 15 ml bottle inside
ME4611 Partition 20 20 20 20 20 For 15 ml bottle outside
ME4305 Coolant 2 2 2 2 2
MB9963 B hose 2 2 2 2 2 φ 12 x 18 x 10 m
MB9961 B hose 2 2 2 2 2 φ 15 x 22 x 10 m
MA7528 Band 4 4 4 4 4
MA1198 A letter of 1
guaranty
ME4498 Binder 1 1 1
ME5234 Operator’s 1
manual
ME6164 Operator’s 1 1
manual
MU9066 Rack ID label 1 1 1 1 1 No. 1 to 20
MU9067 Rack ID label 1 1 1 1 1 No. 21 to 40
MU8051 Rack ID label 1 1 1 1 1 No.1 : 14 pcs
No. 2, 3: 3 pcs

MU9413 Stylus wire 1 1 1 1 1 φ 0.2

ZM0227 Stylus wire 1 1 1 1 1 φ 0.3
ME6332 Tray 1 1 1 1 For GA drain

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Set up preparation 0200

n Standard accessories list

No. Name Japan USA Europe Asia Australia
MB0754 Wrench 2.5 1 1 1 1 1
GB9219 Wrench 1.5 1 1 1 1 1

MU8117 Adapter C 1 1 1 22 pcs/ set ME2272

MU8118 Adapter A 1 1 1 22 pcs/ set ME2175
MU8119 Adapter B 1 1 1 22 pcs/ set ME2176

MU8120 Seal 1 1 1 1 1 Seal 4 types for DC cup

identification

MD7304 Bottle 60 1 1 1 5 5
MD7305 Bottle 30 4 4
ME4609 Bottle 15 4 4
MD0884 Cap A 12 12

ZM0062 Sample cup 1 1 1 1 100 pcs/ set

ME9382 Name plate 3 German

ME9383 Name plate 3 French
ME9384 Name plate 1 German
ME9385 Name plate 1 French
ME9430 Name plate 1 For ODC

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M514 SET UP MANUAL
2-1
Setup flow

n Setup flow

• Selecting the instllation site

• Removing the packing material
• Placing the equipment at the installation site

• Leveling the equipment

• Jacking up the equipment

• Wiring from AU400 power

• Wiring for PC, printer, etc
• Plumbing for supply and waste water

• Pouring coolant (forincubation bath)

Coolant for the DB (cooling unit) has been poured at the factory
before shipment
• Setting the master detergent

** Power ON(PC only)

• Check the BIOS
• Program install
• Setting of System Maintenance
(Refer to System Maintenance manual)
*** After setting, turn off the power

• Process the heater line of the JD portion (to prevent empty burnig)

** Power ON
• Position check by DIAG
• After position check attach the consumables
• Air extraction
*** Power OFF

• Return to processing for heater line of JD

** Power ON
• W1/Photocal
• Water test
*** Analysis test

• Set up complete

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M514 SET UP MANUAL
3-1
Unpacking

n Unpacking
lRemoving the outside packaging materials

1) Loosen the band.

2) Remove the L angle.

3) Remove the ceiling lid.

4) Use a pair of pliers to remove the joint securing

the body frame.

5) Loosen the wire securing the body of the AU400

to the wood frame.

6) Lift the AU400 off the wood frame.

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M514 SET UP MANUAL
3-2
Unpacking

n Opening the package

lRemoving the inside packaging materials

1) Loosen the bands, and remove the vinyl sheet.

2) Remove the grip bar.

3) Remove the all pads to protect the cover.

4) Remove the packaging material for the front door.

5) Remove the screw for fixing the back door.

6) Remove the mouthpiece cover.

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M514 SET UP MANUAL
3-3
Unpacking

7) Remove the packaging fixed to each unit.

8) Peel tapes fixed to the CB(mirror unit).

9) Remove the vinyl sheet fixed to Float SW.

10) Peel the other tapes.

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M514 SET UP MANUAL
3-4
Unpacking

n Standard accessories

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M514 SET UP MANUAL
4-1
Set up procedure

n Leveling
1) Position the floor plates under the jacks. (Position the plates squarely.)
2) Jack up the 4 casters at the same time. If a caster isn’t in contact with the ground, use a level to check that it is
level.
* Open the front door and place the level on the frame.
When checking with a level, place this instrument on the frame after first opening the front door.

3) Use the jacks to make the instrument level.

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M514 SET UP MANUAL
4-2
Set up procedure

n Wiring the power supply

l Wiring the power supply cable between the AU400 and the switchboard of the facility.

! Caution
1.To prevent a short circuit when repairing or assembling, turn off the main power, and wait at least 10 seconds
before starting work.
2. To prevent electric shocks and short circuits do not touch electric parts and wiring with wet hands.

1) Remove the door on the rear side of the instrument.

2) Connect the power supply cable in the region labeled A in Fig. A. (The power supply unit is housed here.)

Region A
3) Connect the power supply cable to the switchboard of the facility.
* Check that the voltage of the facility is Live(Hot) or Neutral(Cold) using a tester surely before the power supply
cable is connected.
* Supply power that conforms to the * Connect to the 3rd
instrument specifications grounding terminal

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M514 SET UP MANUAL
4-3
Set up procedure

n Wiring for personal computer

Make the following connections to the personal computer.

l CRT & PC
CRT Connect to
Power supply [Constant power supply output] for PC
AUDIO IN [LINE OUT] connector for PC
CRT signal [Display] connector for PC
l PC and standard accessories
PC Connect to
Power supply Output from JD portion of ANL
If the PC and printer are both connected to the JD
portion, split the power using the table tap.
Keyboard [KEYBOARD] connector for PC
Mouse [MOUSE] connector for PC
l PC and standard printer
Printer Connect to
Power supply Output from JD portion of ANL
If the PC and printer are both connected to the JD
portion, divide the power by the table tap.
Printer signal [Parallel] connector for PC
l PC and ANL
PC (Communication with ANL) Connect to
Serial connector 1 [COM] connector at JD portion of ANL
l Special accessories and others
Option Connect to
Option printer [Parallel] connector at extension board of PC
Touch panel signal [Serial] connector at extension board of PC
Online [Serial connector 2] of PC

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M514 SET UP MANUAL
4-4
Set up procedure

n Installation of the personal computer

l CRT & PC

CAUTION
1. Do not install the personal computer in an unstable location. There is the danger of injury from
dropping.
2. Do not close the air hole. There is the danger of fire from a stuffed air.
3. Do not istall the personal computer at a location where it is exposed to water or other liquids. There is the
danger of erroneous operation or fire.

l Connection of the personal computer

CAUTION
1. To prevent electric shocks and short circuits do not touch electric parts and wiring with wet hands.
2. Do not insert the power plug into a receptacle while dust or other foreign matter adheres to the plug.
There is the danger of fire.
3. Insert the power plug positively all the way.
4. Contact all cables correctly. A wrong connection can cause trouble.

Connect the ports of the personal computer according to the following table.
For other connections than the following ones, follow the manual for the personal computer.
PC Connecting Remarks
Serial 1 ANL
Serial 2 (Host computer)
Serial 3 Modem Option
Serial 4 Touch panel (expect for OAI) Option
Parallel 1 Printer
Parallel 2 Optional printer Option

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M514 SET UP MANUAL
4-5
Set up procedure

n Plumbing for supply and waste water General diagram

l Connecting to supply water hose, concentrated waste liquid waterh hose, washing waste liquid water
hose, and exhaust air hose.
1) Connect to the blade hoses to the mouthpiece on the rear face. (See the below figure.)
Concentrated liquid water, Washing waste liquid water : Blade hose 15 x 22
Supply water, exhaust air : Blade hose 12 x 18

Instruction
1. Connect to all the hose of concentrated / washing liquid waste, water supply, and exhaust air because a little water is
drained from the exhaust air hose.
2. Don’t be flooded the exhaust air hose in the drain level. If the exhaust air hose is flooded the drain hose in the drain hole,
the depression may be running short.

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M514 SET UP MANUAL
4-6
Set up procedure

n Connecting the deionizer

(1) Connection for MU2535 (M250-OPE01) type

* Allow the deionizer to be connected by an expert distributors.

(2) Connection for MU0815(M240-OPD01) type : for Japan

Connection for MU4146(M520-OPE01) type : for USA
Connection for MU4147(M520-OPE02) type : for Europe
* Allow the deionizer to be connected by an expert distributors.

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M514 SET UP MANUAL
4-7
Set up procedure

n Connecting the forced waste water unit and water supply valve
l Connecting the forced waste water unit
1. Connecting the hose of the forced waste water unit.
Ÿ Connect the waste water line from the main body (hose) to the “ IN ” connector of the forced waste water,
and connect the hose from the “ OUT ” connector to the waste gate of the facility. (The drain hole : 1.5m or
less)
(See Fig. A below.)
Ÿ Connect the tube from the concentrated waste liquid to one forced waste water unit(1st).
Ÿ Put the accessory stopcock to the mouthpiece in one forced waste water unit(1st).
Ÿ Connect the hose from the washing waste liquid and the exhaust air to another forced waste water unit(2nd).
2. Connecting the power supply of the forced waste water unit
Ÿ Connect the power source line and the signal line to the connector of each JD portion. (See Fig B below)

l Connecting the water supply valve (option)

1. G type (solenoid valve type)
Ÿ Connect the tube from the source of the aqueduct to the “ IN ” connector of the solenoid valve, and connect
the tube from the “ OUT ” connector to the input line of the deionizer.
Ÿ Connect the control line of the solenoid valve to the connector of the JD portion. (See the Fig.B below)
* If the resin bomb (cylinder) type deionizer is used, do not connect the solenoid valve to the exit side of the
resin bomb, or the bomb may accidentally burst. Connect it to the entrance side.
2. Mini clear type
Ÿ Connect the control line of the solenoid valve to the connector of the JD portion. (See the Fig.B below)

Fig. A
Fig. B

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M514 SET UP MANUAL
4-8
Set up procedure

n Pouring the coolant

1) Open the cover A. (see the figure below)
2) Remove the stopcock under the cover A, and pour the coolant from the gate.
3) Set the stopcock to the gate.
4) Turn on the environments, and check the coolant level.
* Refer to following pages how to set up the environments.
5) Set the cover A (see the figure below).

n Setting the original detergent

1) Set detergent A and detergent B as shown in the figure below.

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4-9
Set up procedure 0200

n Installation of Drain tube of incubation bath (Except ODC)

1) Drain tube of incubation bath is put in the tray. (ME6332)
2) The tray with the tube is put under the AU400.

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5-1
Set up personal computer

n Setting up the personal computer

1. Turn on only the PC. Do not turn on anything else.
2. Setting the BIOS
* For detailed setting information refer to the system maintenance manual.
3. Installing the AU400 program
* For detailed setting information refer to the system maintenance manual.
4. System maintenance setting
* For detailed setting information refer to the system maintenance manual.
5. If the setup of the PC has been completed, turn of it.

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M514 SET UP MANUAL
6-1
Processing the heater line of JD

n The purpose for processing the heater line of the JD portion

l A new instrument has not primed for the water in the plumbing line. Therefore if the power is turned on for the
first time, the temperature fuse of the heater for wash water heating may trip. Follow the procedure below to
prepare the heater line of the JD portion and prevent the fuse from tripping.

*Note that an error is not issued if the fuse is tripped.

n Preparation procedure
1) Detach the heater wire from the terminal table of the JD portion on the rear left side of the ANL. (See the
below figure A.)
2) Insulate the detached wire with vinyl tape.

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M514 SET UP MANUAL
7-1
Operation check 0200

n Turning on the power

l If the PC has been set up and the JD portion has been processed, turn on the instrument and check the position
and action of each unit.

1. Air extraction of DI water supply pump

1) The front cover opened, and F cover is removed.
2) The connector of the DI water supply pump is removed.
3) The detergent is put in the Detergent tank. (Detergent diluted to 2%.)
4) Turn on the AU400 power.
5) Please wait until the DI tank becomes being filled with water.
6) The filter is removed from the fixing board.
7) The filter is shaken, and water is poured in the pump.
8) The connector of the DI water supply pump is connected.
9) The FC sequence of DIAG menu is done.
10) C insert is removed from C body when doing for a while.
11) Water is turned on pushing the point of C insert to a big container.
12) C insert is installed in C body.
13) The filter is shaken, and sir on the inside is pulled out.
14) Afterwards, water is made to often circulate.
15) The FB sequence of DIAG menu is done.
16) At this time, if the noise dose not do from the DI water supply pump, it is completion.
17) F cover is installed.

CAUTION
The bearing wears out if this work is not done.

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Operation check 0200

2. Air extraction of coolant line

1) The tube of the circulation pump for GA unit in the back of AU400, and air in the tube is pulled out.

CAUTION
1. The error ( INCUBATION BATH TEMP. ERROR etc.) might occur when there is air in the tube.
2. Please confirm there is no air in the tube.

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7-3
Operation check 0200

n Loading the consumables

l Load the sample probe, reagent probe, and mixing bar, by checking the position with the DIAG menu. Do not
load these consumable parts before performing the position check, because the position shift may have been
caused problem.
l The DIAG menu does not have to be used if attaching the adapter for DC unit(the cooling sampler) and
recording paper.

n Position check using DIAG menu

1. Check the horizontal position of the sample probe.

1) Check the sample probe alignment in the cuvette. (Fig. A).
2) Check the sample probe alignment in the wash pot. (Fig. B).
3) Check the sample probe alignment in the sample cup(Rack, DC unit). (Fig. C).
4) Check the sample probe alignment in the ISE sample pot. (Fig. D).

* Check each position and if necessary, adjust according to the Adjustment manual.

B : Wash pot position

A : Cuvette position

C : Sample cup position D : ISE position

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Operation check 0200

2. Check the horizontal position of the reagent probe.

1) Check the reagent probe alignment in the cuvette. (bellow fig. A).
2) Check the reagent probe alignment in the wash pot. (bellow fig. B).
3) Check the reagent probe alignment in the reagent cup. (bellow fig. C).
4) Check the reagent probe alignment in the dilution bottle. (bellow fig. D).

* Check each position and if necessary, adjust according to the Adjustment manual.

B : Wash pot position

A : Cuvette position

C : Reagent bottle position C : Dilution bottle position

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Operation check 0200

3. Check the horizontal position of the mixing bar.

1) Check the mixing bar alignment in the cuvette. (bellow fig. A).
2) Check the mixing bar alignment in the wash pot. (bellow fig. B).

* Check each position and if necessary, adjust according to the Adjustment manual.

A : Cuvette position

B : Wash pot position

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Operation check 0200

4. Check the dead volume for the sample probe and reagent probe.
l Dead volume at rack position
Type Dead volume
Hitachi cup 50µL or less
ACA cup 250µL or less
φ13 blood collection tube 200µL or less
φ15 blood collection tube 250µL or less

l Dead volume at the DC unit position

Type Dead volume
Cup for small quantity 30µL or less
Hitachi cup 50µL or less
Hitachi cup 50µL or less
Conical cup for STAT 50µL or less
ACA cup 250µL or less
φ13 blood collection tube 200µL or less
φ15 blood collection tube 250µL or less

l Dead volume at the reagent bottle

Type Dead volume
15 mL bottle 350 µL or less
30 mL bottle 1mL or less
60 mL bottle 1mL or less

* Check each position and if necessary, adjust according to the Adjustment manual.

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Operation check 0200

n Air extraction

• After making all the position checks, as described on the previous page, recheck the probe and mixing bar
attachment. Carefully check for looseness in the connections of the sample and reagent probe connections
• Check the syringe connection (loose the syringe case or tubes).

1) The sample and reagent dispenser is moved up/down and the air is extracted using the ANL maintenance menu.
* If the air isn’t extracted, detach the syringe case in the dispenser and extract the air by swaying the syringe by
hand.
Air extraction position Menu
Plumbing for sample line Maintenance Ü ANL maintenance Ü S/ R dispenser syringe replacement
Plumbing for reagent line Maintenance Ü ANL maintenance Ü S/ R dispenser syringe replacement

2) The mix sequence is executed and the outer wash water of the mix wash pot is filled using the ANL maintenance
menu.
Air extraction position Menu
Plumbing for mix outer wash Maintenance Ü ANL maintenance Ü Mixing bar replacement

3) The wash nozzle sequence is executed and the wash water is filled using ANL maintenance.
Air extraction position Menu
Plumbing for wash nozzle line Maker maintenance Ü ANL maintenance Ü Replacing Wash Nozzle

Caution
The cuvette may be splashed by water from the nozzle the first time because of air in the tube. In the first
time, detach the nozzle portion necessarily and accept by the container.
Once the air has been extracted, replace the nozzle and check the wash condition by discharging the real
wash water into the cuvette.

4) The probe wash sequence is executed and the wash water is filled using ANL DIAG.
Air extraction position Menu
Plumbing for clot detection sensor Maker maintenance Ü ANL DIAG Ü Probe wash

If the water test (analysis test) is executed without the air having been completely removed the
data may not be reliable. Be sure to extract all the air.

n Processing the heater line in the JD portion

1) If the air has been extracted, turn off the power.
2) Execute the processing for the heater line of the JD portion described on page 6-1.

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8-1
Test running

n Executing W1
1) If processing of the heater line of the JD portion has been completed, turn on the instrument again.
2) When the instrument starts, execute W1.
* For the W1 action, check the wash water discharge and aspiration. (It is easy to check if the cover of the GA
portion is detached.)

n Executing photocal
1) If W1 has been completed, execute photocal by closing the GA portion cover and the main body cover.
2) If photocal has been completed, check the cuvette error.

If the average value of the check range is 0.03, there is no cuvette error.

3) If a cuvette error has occurred, check the applicable cuvette and execute photocal again after cleaning or
replacing it.
4) Keep executing until there is no cuvette error.

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8-2
Test running

n Water test

l If W1 and photocal have been completed, execute the water test as follows and check the photometry line
performance.
1) Enter the parameters as follows.
2) Prepare degassed water (if it is difficult to obtain, use DI water instead) with P8P (triton 100X) to 0.1%. (88
samples x 4 tests).

NAME 340nm 380nm 480nm 600nm

CH R1=2 R1=4 R1=6 R1=8
Amount for sample dispensing 2 2 2 2
Amount for sample dilution 0 0 0 0
Force- multiplier of 1 1 1 1
sample prior-diluent
Amount for R1 dispensing 50 50 50 50
Amount for R1 dilution 100 100 100 100
Amount for R2 dispensing 0 0 0 0
Amount for R2 dilution 0 0 0 0
Measurement wavelength (main) 340nm 380nm 480nm 600nm
Measurement wavelength (sub) 410nm 520nm 660nm 800nm
Analysis method END 1 END 1 END 1 END 1
Reaction slope + + + +
Measurement point 1 1st 1 1 1 1
Measurement point 1 Lst 27 27 27 27
CAL Type MB MB MB MB
Factor 10000 10000 10000 10000

* Others : Set the maximum value, etc.

* Name, CH, and the reagent bottle position : Set in position optionally.
3) Set the 88 sample racks in white cups and put DI water in each cup.
4) Put the degassed water with triton (made in item 2) into the reagent bottle, and place one in the refrigerator.
5) The 4th requisition of the 88 samples is registered in normal test requisition in the routine menu.
6) Start analysis.
7) The data must satisfy the standard below.

Standard The range of 340/410nm, 380/520nm : less than 15 for all channels.
The range of 480/660nm, 600/800nm : less than 10 for all channels.

n Test running
Be sure to perform a water test to check the conforming standard if the photometry and the dispenser system are running
correctly.

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