Two Sample T-Test Statology

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11/17/22, 7:36 PM Two Sample t-test: Definition, Formula, and Example - Statology


M E N U

 APRIL 23, 2020  BY ZACH

Two Sample t-test: Definition, Formula, and Example

A two sample t-test is used to determine whether or not two


population means are equal.

This tutorial explains the following:

The motivation for performing a two sample t-test.


The formula to perform a two sample t-test.
The assumptions that should be met to perform a two sample t-
test.
An example of how to perform a two sample t-test.

Two Sample t-test: Motivation


Suppose we want to know whether or not the mean weight between
two different species of turtles is equal. Since there are thousands of
turtles in each population, it would be too time-consuming and costly
to go around and weigh each individual turtle.

Instead, we might take a simple random sample of 15 turtles from


each population and use the mean weight in each sample to
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11/17/22, 7:36 PM Two Sample t-test: Definition, Formula, and Example - Statology

determine if the mean weight is equal between the two populations:

However, it’s virtually guaranteed that the mean weight between the
two samples will be at least a little different. The question is whether
or not this difference is statistically significant. Fortunately, a two
sample t-test allows us to answer this question.

Two Sample t-test: Formula


A two-sample t-test always uses the following null hypothesis:

H0: μ1 = μ2 (the two population means are equal)

The alternative hypothesis can be either two-tailed, left-tailed, or right-


tailed:

H1 (two-tailed): μ1 ≠ μ2 (the two population means are not equal)


H1 (left-tailed): μ1 < μ2 (population 1 mean is less than population
2 mean)
H1 (right-tailed): μ1> μ2 (population 1 mean is greater than
population 2 mean)

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We use the following formula to calculate the test statistic t:

Test statistic: (x1 – x2)  /  sp(√1/n1 + 1/n2)

where x1 and x2 are the sample means, n1 and n2 are the sample


sizes, and where sp is calculated as:

sp = √ (n1-1)s12 +  (n2-1)s22 /  (n1+n2-2)

where s12 and s22 are the sample variances.

If the p-value that corresponds to the test statistic t with (n1+n2-1)


degrees of freedom is less than your chosen significance level
(common choices are 0.10, 0.05, and 0.01) then you can reject the
null hypothesis.

Two Sample t-test: Assumptions


For the results of a two sample t-test to be valid, the following
assumptions should be met:

The observations in one sample should be independent of the


observations in the other sample.
The data should be approximately normally distributed.
The two samples should have approximately the same variance. If
this assumption is not met, you should instead perform Welch’s t-
test.
The data in both samples was obtained using a random sampling
method.

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11/17/22, 7:36 PM Two Sample t-test: Definition, Formula, and Example - Statology

Two Sample t-test: Example


Suppose we want to know whether or not the mean weight between
two different species of turtles is equal. To test this, will perform a two
sample t-test at significance level α = 0.05 using the following steps:

Step 1: Gather the sample data.

Suppose we collect a random sample of turtles from each population


with the following information:

Sample 1:

Sample size n1 = 40
Sample mean weight x1 = 300
Sample standard deviation s1 = 18.5

Sample 2:

Sample size n2 = 38
Sample mean weight x2 = 305
Sample standard deviation s2 = 16.7

Step 2: Define the hypotheses.

We will perform the two sample t-test with the following hypotheses:

H0: μ1 = μ2 (the two population means are equal)


H1: μ1 ≠ μ2 (the two population means are not equal)

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11/17/22, 7:36 PM Two Sample t-test: Definition, Formula, and Example - Statology

Step 3: Calculate the test statistic t.

First, we will calculate the pooled standard deviation sp:

sp = √ (n1-1)s12 +  (n2-1)s22 /  (n1+n2-2) = √ (40-1)18.52 +  (38-
1)16.72 /  (40+38-2) = 17.647

Next, we will calculate the test statistic t:

t = (x1 – x2)  /  sp(√1/n1 + 1/n2) =  (300-305) / 17.647(√1/40 + 1/38)


= -1.2508

Step 4: Calculate the p-value of the test statistic t.

According to the T Score to P Value Calculator, the p-value


associated with t = -1.2508 and degrees of freedom = n1+n2-2 =
40+38-2 = 76 is 0.21484.

Step 5: Draw a conclusion.

Since this p-value is not less than our significance level α = 0.05, we


fail to reject the null hypothesis. We do not have sufficient evidence to
say that the mean weight of turtles between these two populations is
different.

Note: You can also perform this entire two sample t-test by simply
using the Two Sample t-test Calculator.

Additional Resources

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