Aperio System Requirements (RUO

Version)
This document discusses the recommended hardware and bandwidth requirements for the Aperio
ePathology system.

For Research Use Only. Not for use in diagnostic procedures.

Because Aperio ePathology eSlides are by design high resolution and information rich, for best results you need sufficiently
powerful processing and viewing hardware as well as adequate network bandwidth capacity.

Server Minimum and Recommended Specifications

The DSR (Digital Slide Repository) is the server on which your images are stored. It often is also the location where Aperio
eSlide Manager is installed.

Note on the Service Account

The service account is used by Aperio applications that run as services on the server and scanner.
Make sure that the service account:
`` Is excluded from all password expiration policies.
`` Is given local administrator permissions on the server running the Aperio ePathology services as well as the
scanner.
`` Is given “Full Control” access to any network location where image data is being stored (the file share).
`` Is given DBO (database owner) permissions to the Aperio eSlide Manager SQL database.

Preparing for Windows Updates

Microsoft occasionally issues critical updates that you may want to install on your servers. It is your responsibility to
perform regular backups of your servers. Before installing an update, be sure you have a full backup of the server that you
could use to restore the server in case the update causes issues.

Aperio System Requirements (RUO Version), Revision L © Leica Biosystems Imaging, Inc. 2018 1
Aperio System Requirements (RUO Version)

Cloud/Remote Storage
If you are planning to move Aperio eSlide Manager or image storage to a remote cloud storage service, please contact
Leica Biosystems Technical Service for advice and technical tips first. For performance reasons, we recommend you use
Aperio hub to spoke data transfer to move images to cloud storage instead of scanning directly to a remote Aperio eSlide
Manager hub. For bandwidth requirements for a scanner connected directly to an Aperio eSlide Manager server, see “eSlide
Bandwidth Requirements” on page 6.

Server Prerequisites
As of Aperio eSlide Manager Release 12.4, the following Microsoft Windows patches must be installed on your Aperio
eSlide Manager server for compatibility with PHP 7 and later. If your server is up to date with the latest patches, these
should already be installed on your server:
`` KB2919442
`` KB2919355 (supersedes KB2932046, KB2959977, KB2938439, and KB2934018)
`` KB2999226

Server – DSR Specifications

Component DSR Minimum Specifications DSR Recommended Specifications

CPU speed Two dual-core or one Quad-core Intel 8-core processors 2.0GHz or faster
2.4GHz or faster recommended
Hard disk space 1TB DAS, NAS, or SAN Storage 5TB DAS, NAS, or SAN Storage
(recommended) RAID for redundancy (recommended), RAID for redundancy
Available C: or OS drive space 100GB 100GB
Memory 4GB RAM 16GB RAM
Network card 1GB network card Dual 1GB network cards
Power supply Redundant power supplies Redundant power supplies
(recommended)
Media input Not required DVD (optional)
Operating system WIndows Server 2008 R2 SP1 Windows Server 2012 R2
minimum
`` Image analysis is a CPU-intensive activity, and so we recommend your DSR use the recommended specifications
for best performance. Image analysis users may also want to talk with Leica Biosystems Technical Services about
using the Aperio Image Analysis Software Accelerator or Aperio Image Analysis Hardware Accelerator to boost
image analysis performance.
`` You are encouraged to provision your DSR following the recommended specifications to provide the best
performance experience for your customers.
`` Note that installation procedures may require temporary active Internet connection and access to a Windows
update service.

2 Aperio System Requirements (RUO Version), Revision L © Leica Biosystems Imaging, Inc. 2018
 Aperio System Requirements (RUO Version)

Using a Virtual Machine as Your Aperio eSlide Manager Server (DSR)

If you are using a virtual machine (VM) as the server, you will see better performance in a virtual environment if your VM
meets the recommended specifications shown in the table above and have dedicated resource allocation on the virtual host.

Supported Server Operating Systems and Language for Aperio eSlide Manager and
Aperio Image Analysis Workstation
64-bit support refers to using installers built from 32-bit compilers and installed on 64-bit OS.
We do not support the localization of our software.
To ensure proper operation of Aperio ePathology software, the region setting of DSRs, the scanner control workstation, and
client workstations should be English (United States), set in the Windows Regional and Language Options window
available from the Windows control panel. In addition, the English language Windows pack should be installed on the DSR.

Operating System OS Bit Native Language Support Release

Windows Server 2003 32 US English, Spanish Rel 9.x, 10.x, 11.x
Windows Server 2008 R2 64 US English, Japanese, Spanish, German Rel 11.x, 12.x
Windows Server 2008 R2 SP1 64 US English, Japanese, Spanish, German Rel 12.3 and later
Windows Server 2012 R2 64 US English, Japanese, Spanish, German

Supported Database Software for Aperio eSlide Manager and Aperio Image Analysis
Workstation

Database Service Pack Release

MS SQL Server 2005 SP3 Rel 9.x, 10.x
MS SQL Server 2008 R2* SP2 Rel 11.x, 12.x (pre-12.3)
MS SQL Server 2014 SP2 Rel 12.3 and later

*Microsoft extended support is available through 2019.

Scanner Control Workstation

The Aperio ePathology scanner system consists of the combination of the scanner and the computer attached to that
instrument, the scanner control workstation. Do not use the scanner control workstation for any other purpose—it is lab
equipment. Both the scanner and its control workstation must be functioning for scanning to occur.

Aperio System Requirements (RUO Version), Revision L © Leica Biosystems Imaging, Inc. 2018 3
Aperio System Requirements (RUO Version)

Client Workstation Specifications

Viewing Station Requirements – Minimum

Component Aperio eSlide Manager Aperio Image Analysis Workstation

CPU speed Intel Core 2 Duo (or newer) processor, running at Intel Core 2 Duo (or newer) processor, running at
2GHz or faster recommended 2.7GHz or faster recommended
Hard disk space 40GB free disk space 100GB free disk space
Memory 4GB or more recommended 4GB or more recommended*
Network card 1 Gigabit network card or faster 1 Gigabit network card or faster
Video card 24-bit color at monitor’s resolution 24-bit color at monitor’s resolution
Operating Windows Pro 7 64-bit, Windows 8.1 64-bit, Windows Pro 7 64-bit, Windows 8.1 64-bit,
system Windows Pro 10 64-bit Windows Pro 10 64-bit

*Users working with large whole slide images (>1GB such as multiple FL channels or z-stack layers) are
recommended to use 8GB or more of RAM to minimize performance issues. For best performance, we
recommend that images be opened from the Aperio Image Analysis Workstation eSlide list page instead of
directly from the file system.

Monitor Requirements
The following requirements are for Aperio eSlide Manager and Aperio Image Analysis Workstation. The recommended
specifications will provide a better viewing experience than the minimum specifications.

Component Minimum Specifications Recommended Specifications

Display type LCD (flat panel) LCD (flat panel)
Screen resolution 1680(h) x 1050(v) pixels 1920(h) x 1050(v) pixels
Screen size 24-inch 24-inch or larger
Color depth 24-bit 24-bit
Brightness 250 cd/m2 or greater 250 cd/m2 or greater
Contrast ratio 500:1 1000:1

Supported Web Browsers for Aperio eSlide Manager and Aperio Image Analysis
Workstation
Other browsers and browser versions may work, but they have not been exhaustively tested by Leica Biosystems Imaging.
Note that Firefox ESR (Extended Support Release) is not supported for use with Aperio eSlide Manager and Aperio Image
Analysis Workstation.

4 Aperio System Requirements (RUO Version), Revision L © Leica Biosystems Imaging, Inc. 2018
 Aperio System Requirements (RUO Version)

Web Browser Versions Aperio Release

Internet Explorer 7.x, 8.x Rel 9.x, Rel 10.x
8.x Rel 11, 11.1
8.x, 9.x 11.2
8.x, 9.x 12.x (IE9 recommended)
11.x Rel 12.3 and later
Firefox 2.x Rel 9.x
3.x Rel 10.x, Rel 11, Rel 11.1
12.x Rel 11.2
28.x Rel 12.x
37.x - 47.x Rel 12.3 and later
Chrome 56 Rel 12.4 and later

Adobe Flash Player Legacy Support

Aperio is phasing out the use of Adobe Flash Player because of its security vulnerabilities. Additionally, major Internet
browsers (Chrome, Firefox, Internet Explorer) have begun to phase out Adobe Flash Player in favor of HTML5 solutions, and
now require installation of the latest Flash Player and extra steps to enable its use.
As of the Aperio eSlide Manager 12.4 release, the following eSlide Manager features still use Flash Player: TMALab map,
WebScope image viewer, attaching documents to Cases, and uploading gross images to Specimens. If customers are using
these features, it is important that they make sure they are using the latest Flash Player and have enabled its use in their
browser.
Note that Aperio WebViewer (which does not use Flash Player) is a browser-based alternative to WebScope and provides
all the essential viewing capabilities within an HTML5 environment. For more on the Aperio WebViewer, refer to the Aperio
eSlide Manager Operator’s Guide.
For details on installing and enabling Flash Player, refer to the document Internet Browser Support for Adobe Flash Player in
Aperio Products.

Anti-Virus Exclusions for Servers and Workstations

We highly encourage the use of antivirus software on the Aperio ePathology system. We recommend that .SVS, .SCN, .TIF,
JPG file types as well as the file storage be excluded from “on access scanning” as these files can be very large and are
accessed continually as they are being scanned and users are viewing the eSlides. You should also exclude the database
files (.mdf and .ldf) from virus scans. Virus scans should be configured to run during non peak hours as they are very CPU
intensive and interfere with scanning or analysis.

Possible Third-party Application Conflicts

In rare circumstances, third-party applications such as virus or security software may prevent Aperio software from
connecting to servers or devices. If you are having this problem, contact Leica Biosystems Technical Services for assistance.

Aperio System Requirements (RUO Version), Revision L © Leica Biosystems Imaging, Inc. 2018 5
Aperio System Requirements (RUO Version)

Cybersecurity and Network Recommendations

For information on requirements and recommendations for ensuring the Aperio eSlide Manager server on your network and
the users who use it are protected from security threats, refer to Aperio Cybersecurity and Network Recommendations. For
specific cybersecurity recommendations for user workstations, see the user guide for the Aperio product you are using.

eSlide Bandwidth Requirements

The bandwidth required for different applications varies, as discussed below.
Aperio eSlide Manager: When using Aperio ImageScope, the data for the viewing session is streamed to the end user.
First the user is provided the view that has been requested, and then surrounding views are cached in anticipation that the
user will request them. The ability to stream this data to end users greatly improves their viewing experience and allows
multiple users to easily share even low bandwidth connections while viewing eSlides. These streaming options can be
managed and are described in the Aperio ImageScope User’s Guide.

eSlide Viewing for SVS and Third-Party eSlides

The minimum network bandwidth required for a good user experience when viewing SVS eSlides is 2 Mbps per user. When
it’s available, our software will make use of additional available bandwidth to provide the users a better viewing experience.
Actual bandwidth usage when viewing eSlides varies with the type of slide, viewing habits of the Pathologist, tissue
area, nature of each case, the client application and performance options in the viewer application. However, based on
experience, Aperio has found that a typical viewing session requires about 25MB of data to be transferred to the end user
with a variable rate of 2 Mbps to 4 Mbps.

eSlide Viewing for SCN eSlides

The minimum network bandwidth required for a good user experience when viewing SCN eSlides is 24 Mbps per user.
When it’s available, our software will make use of additional available bandwidth to provide the users a better viewing
experience.
Actual bandwidth usage when viewing eSlides varies with the type of slide, viewing habits of the Pathologist, tissue area,
nature of each case, the client application and performance options in the viewer application.

eSlide Capture on Aperio Scanners

We recommend that the connection between the scanner and DSR be low latency and dedicated to scanning activities. If
the connection is not dedicated, scanning performance may be impacted or may impact other applications on your network. 
The amount of bandwidth required for scanning eSlides to a network is 1Gb/s for optimal scanning time in environments
where the connection cannot be dedicated or is shared. In instances where multiple scanners are writing to the same
network share, these connections may need to be scaled up appropriately. (See “Aperio LV1 Live View and Desktop Scanner
Bandwidth Requirements” below for bandwidth information specific to the Aperio LV1 Live View and Desktop Scanner.)

Scanner Live Viewing

The Aperio scanner TelePath Live feature requires higher network transfer speeds than eSlide viewing. A typical data rate
for live viewing is about 3.2 Mbps, and requires about 250MB of data to be transferred to the user.

6 Aperio System Requirements (RUO Version), Revision L © Leica Biosystems Imaging, Inc. 2018
 Aperio System Requirements (RUO Version)

Aperio LV1 Live View and Desktop Scanner Bandwidth Requirements

The recommended minimum bandwidth to use the Aperio LV1 is with an internet connection of 50 megabits per second
(Mbps) or higher, as this is how the system was validated. The minimum bandwidth includes an upload speed of at least 50
Mbps and a download speed of at least 50 Mbps, both where the physical Aperio LV1 is connected and where the remote
user is connected remotely to control the Aperio LV1.
If the bandwidth speed is lower than 50 Mbps, the user can expect a slower refresh rate of the images being remotely
viewed. Consequently, it is recommended to confirm if a refresh rate slower than 50 Mbps is acceptable to the potential
users of the Aperio LV1 by conducting a live demonstration in the real IT environment in which the LV1 will be used.

Aperio System Requirements (RUO Version), Revision L © Leica Biosystems Imaging, Inc. 2018 7
Aperio System Requirements (RUO Version)

Aperio System Requirements (RUO Version)

MAN-0052, Revision L | 9 April 2018
This document applies to Aperio eSlide Manager Release 12.4 and later
Copyright Notice
ÌÌCopyright © 2017-2018 Leica Biosystems Imaging, Inc. All Rights Reserved. LEICA and the Leica logo are registered trademarks of Leica
Microsystems IR GmbH. Aperio is a trademark of the Leica Biosystems group of companies in the USA and optionally in other countries. Other
logos, product and/or company names might be trademarks of their respective owners.

Customer Resources
ÌÌFor the latest information on Leica Biosystems Aperio ePathology products and services, please visit www.LeicaBiosystems.com/Aperio.

Disclaimers
ÌÌUse normal care in maintaining and using Aperio ePathology servers. Interrupting network connections or turning off the servers while they are
processing data (such as when they are analyzing eSlides or generating an audit report) can result in data loss.

ÌÌThis manual is not a substitute for the detailed operator training provided by Leica Biosystems Imaging or for other advanced instruction. Leica
Biosystems Imaging Field Representatives should be contacted immediately for assistance in the event of any instrument malfunction. Installation
of hardware should only be performed by a certified Leica Biosystems Imaging Service Engineer.

ÌÌImageServer is intended for use with the eSlides created by scanning glass slides with the scanner. Educators will use Aperio ePathology software
to view and modify eSlides in Composite WebSlide (CWS) format.

Patents
ÌÌAperio ePathology products are protected by U.S. Patents: 6,711,283; 6,917,696; 7,035,478; 7,116,440; 7,257,268; 7,428,324; 7,457,446; 7,463,761;
7,502,519; 7,518,652; 7,602,524; 7,646,496; 7,738,688 and licensed under one or more of the following U.S. Patents: 6,101,265; 6,272,235;
6,522,774; 6,775,402; 6,396,941; 6,674,881; 6,226,392; 6,404,906; 6,674,884; and 6,466,690.

Contact Information – Leica Biosystems Imaging, Inc.

Headquarters Customer Support General Information

Leica Biosystems Imaging, Inc. US/Canada Tel: +1 (844) 534-2262 (toll free) US/Canada Tel: +1 (866) 478-4111 (toll free)
1360 Park Center Drive Direct International Tel: +1 (760) 539-1150 Direct International Tel: +1 (760) 539-1100
Vista, CA 92081 US/Canada/Worldwide Email: Email: [email protected]
USA [email protected]
Tel: +1 (866) 478-4111 (toll free)
Direct International Tel: +1 (760) 539-1100

European Union Authorized Representative

CEpartner4U
Esdoornlaan 13
3951 DB Maarn
The Netherlands

8 Aperio System Requirements (RUO Version), Revision L © Leica Biosystems Imaging, Inc. 2018