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    XX063

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    This document summarizes the results of a sperm DNA fragmentation (TUNEL) assay. The assay found a DNA fragmentation index (DFI) of 15-25% for the patient, indicating best to good sperm DNA credibility. A semen analysis also found a sperm concentration of [value] million/mL. The TUNEL assay evaluates sperm cells under fluorescence microscopy to detect damaged DNA. A DFI between 15-25% generally indicates good sperm DNA credibility and fertility potential. High levels of DNA fragmentation can increase risks of failed fertilization, miscarriage, or genetic abnormalities.

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    XX063

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    Bittu
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    This document summarizes the results of a sperm DNA fragmentation (TUNEL) assay. The assay found a DNA fragmentation index (DFI) of 15-25% for the patient, indicating best to good sperm DNA credibility. A semen analysis also found a sperm concentration of [value] million/mL. The TUNEL assay evaluates sperm cells under fluorescence microscopy to detect damaged DNA. A DFI between 15-25% generally indicates good sperm DNA credibility and fertility potential. High levels of DNA fragmentation can increase risks of failed fertilization, miscarriage, or genetic abnormalities.

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    Sperm count test report

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    This document summarizes the results of a sperm DNA fragmentation (TUNEL) assay. The assay found a DNA fragmentation index (DFI) of 15-25% for the patient, indicating best to good sperm DNA credibility. A semen analysis also found a sperm concentration of [value] million/mL. The TUNEL assay evaluates sperm cells under fluorescence microscopy to detect damaged DNA. A DFI between 15-25% generally indicates good sperm DNA credibility and fertility potential. High levels of DNA fragmentation can increase risks of failed fertilization, miscarriage, or genetic abnormalities.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    40 views3 pages

    XX063

    Uploaded by

    Bittu
    This document summarizes the results of a sperm DNA fragmentation (TUNEL) assay. The assay found a DNA fragmentation index (DFI) of 15-25% for the patient, indicating best to good sperm DNA credibility. A semen analysis also found a sperm concentration of [value] million/mL. The TUNEL assay evaluates sperm cells under fluorescence microscopy to detect damaged DNA. A DFI between 15-25% generally indicates good sperm DNA credibility and fertility potential. High levels of DNA fragmentation can increase risks of failed fertilization, miscarriage, or genetic abnormalities.

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    of 3

    REPORT FORMAT

    Name: -- Lab No.: --

    Age/Gender: -- Years/ MALE Reporting centre: --

    Referring clinician: --
    Sample collected: --
    Sample received: --
    Reporting date: --

    SPERM DNA FRAGMENTATION (TUNEL) ASSAY, FLUORESCENT MICROSCOPY


    SPECIMEN
    Semen

    Clinical Indication
    To rule out sperm DNA fragmentation

    Result Summary
    DNA FRAGMENTATION INDEX (DFI): ---%

    Interpretation
    Sperm DNA fragmentation assessed by TUNEL assay has found to be 15-25% corresponding
    to best to good sperm DNA credibility.

    SEMEN ANALYSIS

    • Concentration of sperm per mL of ejaculate: ---million/mL


    • Viability:
    TUNEL ASSAY ANALYSIS
    Total number of sperm cells Number of TUNEL Number of TUNEL Positive
    evaluated Negative sperm cells sperm cells

    200 -- --

    TUNEL assay for detection of sperm apoptosis. Under fluorescence microscopy normal DNA (non
    apoptotic sperm cells) were seen light green and damaged DNA (apoptotic sperm) is seen bright green (×
    100 eyepiece magnification).

    Page 1 of 3
    Name:
    DNA Fragmentation Index (DFI):

    Number of TUNEL negative morphologically normal sperm X 100


    Total number of sperm cells evaluated

    Four statistical types of fertility potency:


    Less than or equal to 15% DFI outstanding to sound sperm DNA credibility
    Between 15 to 25% DFI best to good sperm DNA credibility
    Between 25 to 50% DFI good to weak sperm DNA credibility
    Greater than or equal to 50% DFI exceptionally poor sperm DNA credibility

    Methodology
    DNA fragmentation in spermatozoa were evaluated simultaneously using a modified TUNEL
    assay associated with FISH.

    Recommendations
    • Chromosomal analysis and sperm aneuploidy detection are advised, if not performed
    earlier.
    • Genetic counselling is recommended.

    Comments
    • Test results should be interpreted in the context of clinical findings, family history, and
    other laboratory data. Misinterpretation of results may occur if the information provided is
    inaccurate or incomplete.
    • Male infertility due to causes other than sperm DNA fragmentation test, has not been
    excluded.
    • High levels of DNA fragmentation may be reduced by considering varicocele repair,
    treatment of underlying infections or inflammation, changes in lifestyle, or with antioxidant
    supplements.

    Indications for Testing


    The test is useful in males diagnosed with non-obstructive azoospermia or moderate to
    severe oligospermia, idiopathic infertility and those undergoing testicular sperm extraction
    (TESE) or prior to ICSI/IVF.

    Clinical implications of Sperm DNA fragmentation assay


    • The assay offers a potentially useful way to examine the ability of sperm to interact
    successfully with an egg that goes beyond the standard semen parameters of density,
    motility, and morphology.
    • Sperm DNA fragmentation is higher in subfertile men with abnormal sperm parameters.
    • This assay provides a reliable analysis of sperm DNA integrity that may help to identify
    men who are at risk of subfertility.
    • The assay helps in the clinical diagnosis, management and treatment of male fertility.
    • It provides prognostic value in assessing the outcome of assisted conception treatment.

    Page 2 of 3
    Name:
    • High rates of sperm DNA fragmentation and pregnancy potential.
    • Embryos derived from sperm with highly fragmented DNA have poor prognosis.
    • An elevated DNA fragmentation could result in initiation of apoptosis (natural cellular
    death) and mutations resulting in blastocyst arrest, miscarriage and abnormalities in the
    offspring.

    Note
    This is a Laboratory developed test (LDT), developed at Dr. Lal PathLabs and has not been
    cleared or approved for specific uses by FDA.

    References
    • Arumugam M, Shetty DP, Kadandale JS, Nalilu SK. Association of Sperm Aneuploidy
    Frequency and DNA Fragmentation Index in Infertile Men. J Reprod Infertil. 2019;
    20(3):121-126.
    • Chen Q, Zhao JY, Xue X, Zhu GX. The association between sperm DNA fragmentation
    and reproductive outcomes following intrauterine insemination, a meta analysis. Reprod
    Toxicol. 2019; 86:50-55.
    • Evenson DP, Wixon R. Clinical aspects of sperm DNA fragmentation detection and male
    infertility. Theriogenology. 2006; 65(5):979-991.
    • Sharma R, Masaki J, Agarwal A. Sperm DNA fragmentation analysis using the TUNEL
    assay. Methods Mol Biol. 2013; 927:121-136.

    Dr. Saurabh Kumar Bhattacharya Dr. Leena Rawal Dr. Kamal Modi
    Ph. D Cytogenetics Ph. D. Molecular Genetics MD, Biochemistry
    HOD Scientist, Cytogenetics Consultant Biochemistry
    NRL-Dr Lal PathLabs Ltd

    ******End of Report*****

    Page 3 of 3
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