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    GPAT Microbiology Syllabus

    Uploaded by

    kumar Harsh
    The GPAT Microbiology Syllabus covers 9 key topics: 1. Introduction to microbiology including the history and contributions of key scientists. 2. Microscopy techniques including different types of microscopes and staining methods. 3. Biology of microorganisms including bacterial growth, genetics, and pathogenic species. 4. Fungi and viruses including their structures, industrial and medical significance. 5. Aseptic technique including sources of contamination and prevention methods. 6. Sterilization and disinfection including various methods and their mechanisms of action. 7. Microbial spoilage including factors affecting pharmaceutical products. 8. Vaccines and sera including their manufacturing, quality control, and types.

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    GPAT Microbiology Syllabus

    Uploaded by

    kumar Harsh
    223 views2 pages
    The GPAT Microbiology Syllabus covers 9 key topics: 1. Introduction to microbiology including the history and contributions of key scientists. 2. Microscopy techniques including different types of microscopes and staining methods. 3. Biology of microorganisms including bacterial growth, genetics, and pathogenic species. 4. Fungi and viruses including their structures, industrial and medical significance. 5. Aseptic technique including sources of contamination and prevention methods. 6. Sterilization and disinfection including various methods and their mechanisms of action. 7. Microbial spoilage including factors affecting pharmaceutical products. 8. Vaccines and sera including their manufacturing, quality control, and types.

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    The GPAT Microbiology Syllabus covers 9 key topics: 1. Introduction to microbiology including the history and contributions of key scientists. 2. Microscopy techniques including different types of microscopes and staining methods. 3. Biology of microorganisms including bacterial growth, genetics, and pathogenic species. 4. Fungi and viruses including their structures, industrial and medical significance. 5. Aseptic technique including sources of contamination and prevention methods. 6. Sterilization and disinfection including various methods and their mechanisms of action. 7. Microbial spoilage including factors affecting pharmaceutical products. 8. Vaccines and sera including their manufacturing, quality control, and types.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    223 views2 pages

    GPAT Microbiology Syllabus

    Uploaded by

    kumar Harsh
    The GPAT Microbiology Syllabus covers 9 key topics: 1. Introduction to microbiology including the history and contributions of key scientists. 2. Microscopy techniques including different types of microscopes and staining methods. 3. Biology of microorganisms including bacterial growth, genetics, and pathogenic species. 4. Fungi and viruses including their structures, industrial and medical significance. 5. Aseptic technique including sources of contamination and prevention methods. 6. Sterilization and disinfection including various methods and their mechanisms of action. 7. Microbial spoilage including factors affecting pharmaceutical products. 8. Vaccines and sera including their manufacturing, quality control, and types.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    GPAT Microbiology Syllabus

    1. Introduction to Microbiology

     Scope and application to pharmacy field. Whittaker’s Five Kingdom concept,


    historical development – biogenesis Vs. abiogenesis, Germ theory of
    fermentation, Germ theory of disease, the contribution of Leeuwenhoek,
    Robert Koch, Jenner, Louis Pasteur and Ehrlich.

    2. Microscopy and staining technique

     The principle, ray diagram, construction, working and applications of light


    compound, dark field, phase contrast, Fluorescence & electron microscope.
    The concept of resolving power, Magnification power, numerical aperture and
    angular aperture and working distance. The principle application of oil
    immersion microscopy. Theory of staining, principle and technique of staining
    procedure – Monochrome, Gram, acid-fast, negative, capsule, endospore.

    3. Biology of Microorganisms

    Cultural characteristics, pure culture techniques

     Bacteria – Morphology and fine structure of bacteria, Nutritional requirement


    and type of culture media, growth and growth curve of bacteria, physical
    condition for growth, measurement of bacterial growth (Counting Methods),

     Reproduction in bacteria, genetic exchange – transformation, conjugation, and


    transduction, development of drug resistance by recombination and mutation,
    preservation of bacterial culture. Biochemical properties (sugar fermentation
    and IMVIC test). Pathogenesis of Staphylococcus, Mycobacterium. Salmonella
    Introductory study of disease-causing rickettsia, the importance of
    actinomycetes in antibiotic production.

    4. Fungi and Viruses

     Fungi:- Introduction, general characteristics, morphology, the industrial and


    medical significance of Saccharomyces Cerevisae, Penicillium and Aspergillus,
    Candida Albicans, Epidermophyton, and trichophyta.

     Viruses: – Introduction, structure and general properties Bacteriophages –


    Lytic and Lysogenic cycle, Epidemiological uses of Bacteriophages, human
    viruses – Cultivation and Multiplication virus-host cell interaction,
    Pathogenesis of HIV and Prions, types of Tumor viruses.

    5. Aseptic Technique

     The omnipresence of microorganisms, the importance of asepsis, sources of


    contamination and methods of prevention, Principle, construction & working
    of laminar airflow bench.

    6. Sterilization & Disinfection

     Concept and classification, principle and methods of sterilization, Mechanisms


    of cell injury.

     Construction working & applications of moist heat & dry heat sterilizer,
    gamma radiation sterilizer, filtration sterilizer. Indicators of sterilization,
    microbial death, kinetic terms-D value, z value.

     Terminology of chemical antimicrobial Agents, Chemical classification of


    different disinfectants, characteristics of ideal disinfectants, factors affecting
    the action of disinfectants, evaluation methods (RW Coeff.), Kelsey Sykes test,
    Chick Martin test.

    7. Microbial spoilage

     Types of spoilage, factors affecting spoilage of pharmaceutical products.

    8. Vaccines & Sera

     Manufacturing (seed lot system) and quality control of bacterial vaccines &
    Toxoids (Tetanus, TAB, Cholera, BCG, DPT), Viral vaccine (Polio- Salk
    Sabin, Rabies, MMR, Hepatitis, Chickenpox, influenza), Antisera (diphtheria,
    tetanus), antiviral Antisera (rabies). Preparation of allergenic extracts &
    diagnostics.

    9. Microbial Assay

     Importance, general methods of assay of antibiotics (Cup & plate method,


    paper disc method, turbidometry, dilution method), methods for fungicidal &
    antiviral compounds, assay, microbial limit tests.

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