Octahadsal

Date 2.3 719

Expt. No. Page No.

EXPERIMENT-10
iN e phuqasa caMstalk ekDetash aluna LEitkahi
LK,SD A, (SDg :24H,DJ
Thue
Petaah MLNAALd bs_disseluing_an eauinwelah MLA
hdsad alunuinum uphal and petassium bulphal in
r anAeund wlis containinga te h Bulhuk
acid and fhun subiedting fhsuing saiuien to aystallisatio
han ectahudsal hustads petash alum Sepesa eut

,S+HSD,)28H,0 +6H,D k, SDl(SD 24H,D.

luse beakISs L2.So m), china -dish sunnal funne-sand, glass-50d

Looskh bottle ipod stand, ond oisL gauze..
Potassium ulphat alununiwn sulpha and dil. sulphuaic acid

Phoduss
lak a 250ml beakus. LWash it with oalh and thun 5ansfs
2:S4 petassium ulphai thystals to it. Pdd abeut 20 ml
o tAShs to_disselue tha thiustalsLWas i uibtd

2.Tak h ofhuk 25D mbeaksh 0s it oifh als and thun

Eonss Dg alununiun ulphd ushs w it Prcdd about
20l e uod and Im of d Sulphusic acid to psurent

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 Date

Expt. No. Page No.

hudselusi aunainiurm sulphati. Heat fesabeut S nins.

Milkinex still nussidi. Alit th solutien..
Miz he Seutiens in a china-dish and plac ha Cnuna
dish em h
gAL2eplaced eves a busnus.Shh
Selhen oh a alass-sed, Concsnia 1Ra selunen nll 1a
ustallisatien peint is ah Plac the dish M
beakes Lontauxung cekd wali.

Seen fh (uslals petash alumsepasat cut Hecant

Th sthisliaue& and uoash he tystals with a Small
auantity eica-celd sals
Bsy ho ustak by placing thuna beteen b_papes pads
bby phiading hna e peeus plal
Obsewoiens
eight eh custals ebtainzdB:6g
Expectideld - 108
Celeus thu hsials celownkos

Shape oh th custals octahadaa.

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 Date

Page No.
Expt. No.

Pucauieng
Cee fhe seButiens sleust to get geod Chstals

Cetkad
met dishusb ha bolutien whilit is being

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KEpCTIONS
Fessic chkleside Test
FeC +6CcHsOH f e (oc,H,), +3HO
Fessuic Phunet euViolet Complex
chleside
 Date 21719
Expt.No.
Page No.

ExeeRIMENT
Pina Tdunhfy ha funchiona eganic
heupwn thGANen
Cempetund.

Phusical slat kiauid

Celeuh- Celenuskuss
Ddeua- OdesAuk
Selubilihy-inseluik in_uol
Ohsesva-iens
Sna Expexununt Obsekwahen Inesanee
Addud blue inwus Sel usng blua to nay Lentain.
to fhu gien ganic d Cas.bouycand a
Cempeuwnd. pharelic Aup

2 Pdd pinch anueunt e b No bsisk MAa onteun

NaHCD, tp tho quen eaescence phaneliceup
Aganic C9mpeund

3 hdd 2-2 m emuikal Viol celeuA Tm Contain

phasechlesids to th phunelic eup
9ANen 9AanicCempetund. Ccontsnaud

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 Date

Expt. No. Page No.

S.no Expesinent 9bsanatien Infeaane

Addid ushic anuenium BAALonceleusd

Phanelic gsup
nikair o the gien eganicSubpsion Lonisad
Lempeund
Phunelic gseup
sAddud 2-3ml oh bbenuna Ohilt pp
Cenisuad
walls o the aien hganic
Lempeund

Ruslt containsPaeNOLICaseup
gien hganic tempound
h

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 RERCTIONS
Gussic anunveniun nikai s t
2R-DH NH) Ce CN0,) TROH), Ce CND,)t
Aloehel Owuenum Pink Ad 2NMND
iRala
complex
2 C-OH +(NH), le (No,) g TCe Dr), Ce 2 NHND
PinR 9 ho
omplex.
 Date 6 6lia
Expt. No.
Page No.

EXPERIMENT 2
Ain e iduntify a fun.cheral gheup n
u
hganicCempeun
Physical btati-kiauiid
Coleua Leleuhus
Odeuh edeuslue
Selubilihy Sekuble in Loal

9bsesyaien
Sno Expesineni Obeeswaien Tnfaanca
Addd bluu lihuus seluhon will net Pbsene eh
to ha ginen hgeLnic tuan blu to Ad. lcasboaylic acid
LPmpeunds andphunelic gseup

Rdd pinch anueunt eh NaHCo, le bsisk Pbsen

hehe 4en cempeund eASCunce

3Add 2-3 m nuilkal Ne celUN Phunelic 9sSup

phsachloidle to hs empeund absent

4Add sic aurwenium nikafi Pink d celeus alcehe gseup

to he QiNen cempeund.. pALLent

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 2
Esti Test
HT
R-OH +RR--OH R'-C-0-R+ H,0
Plcehel akbouylic acid Esto

H
CH DH + CH-C- OH CH-C0-CH+ H,0
Mathuy Hcehc acid MAfu ocetati
alcehel

1oderesm Test
OH

CH-CH-CH+ I, +2 NaOH CH-C-CH,+2 NaL +2H,0

2- Ropanel

CH-C-CH+8T, + NaD CHT +CHH,UODNA+3Nal +3H,0

Auten Todorem
 Date

Expt. No. Page No.

Sna ExpesinLnl 9hsesNatien IofesuncL

Estt Test
AddLd -2 m bcutic arid OH 95.sup
huiby S
-2 ml e ganic Conism
compplund,
uo dsops encankad
sulphubic acid

LOnsM he nizhu in_ uoali

horh

eel 6peus fh Conari into

iD m o 57 sodium
Casbenn suien in ha
beakis

LDdeeM est
hddid sediunn hydsoxids ulles ppt Tdossm (CHI)
Todids selutien to Aganic OReup phisent
Substonc

Pusukt
Tw
h quen gnnic Cempeund Lontains ALcoHOLIC
ghep

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 KeeCTIONS
2, 4 DNP Tost
HO
a H

CH c =0 t
H,N-NH(ONO
Pataldehuds CH-C=N-NH-O-NO
No No
2,4-ODinuiophuy Rctuldihyd 2,4-dinihaphural
hydkazire nuy dka.z.en

cH3
CH
CH = o +H,N-NH ONO, CHN-NHO-NO
NO CH3
Pcatalduhude NO
2,4-Dinuthephur
hudsazik Hacbene-2,4-iri.Rophuny
hydsa ze
 Date 26
Expt. No. 13 Page No.

ExPERIMENT-
i Ldaily tha fun.ctienal qsoup hibent in hu
haanic CeMpeund.

Physical stat kiauid

Celouh cdleunluss
Ddeus odeunls
Selubilkhy -seluble ins w

bses vath9n

S.no Expesinunt 9hseswatien Tnfsunc

Ne chnge Phuneic and

ceuien te ha qAen casbozuyic acid
hoanic Cempeund. qpup absent.

2.Pad ussicaAeium Ne hid celeu Alceh.eliceup

mika sution 2-3 absent
dseps t th aien.
Sonuple).

3Addud 2,4-DNP Yelleo eheAangsanbenul 9sup-

Ldini.Bephanyl hudbazina). Cuystak Nd phistnt
shak well and allew
to stand
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) Toon's TC&
2 Ha CNHs), tRCH0 + 80H RCO0 +2hgv +NH+2

2 Ag NHS) +CH, CH0 t 30H CH,CO0+2Pq +4NHt 2H.0

Aatalduky Aatai

Fehlang's Test
R CHO +2Cu+50HT
Pldshyds
Cu,Ots)+RCO0+3H,0
Rd)
Fehling's Seluhon
 Date

Expt.No.
Page No.

N0 Expesinunot 9hseswatier TofoueL

Addud 2-&m e Sodium (aystallina

asberu eup

bisulphik tD fh guxen SaoplephLcipitalL pnad CenisM

shaR ell alleused to stand
febuoninkA

S Schif suagnt Tut Silwa isseh ALduhyd oneup

Addud 2 m eh sdhif (shining uissA)
NAGLn te ths qun Sample.hd

eun'Shagen tus Silws mshPh Alduhuds conisnud

Addd -2 ml of tellens Lshining usseh)
iagnltD Tha ANen Sample. onad

1Fohin s Solutien
Aadc 2ml eFehlings Redceleu Ptduhyde-CHO)
selutien A and B to tha gacnlRud ppt)ndLonfihead
SanAple and wabm en OaA
boh

Pubulks
ien hganic Cempund contains HLDEHYDE (-CHO)
9heuLp

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 RLachiens
. 2,4 ONP Test

CH-C=0 +H, NNH- DN0, CH C=N O-NO

Acuteld hyee NO NO
2,4-Dinikephun tal duhyds 2,4
udsazine hy dha28na
dinilliophun2

Chs C-0+ H,N-NHOOND CHcN-H O-NO,

CH CNS
NO NO
Patalduhude
2,4-DiikephA Pcaene-2,4-diniuBophunuy
hudha Suu huyosa 29ra
 Date 26 B119

Expt. No. 14 Page No.

ExeERIMENT14
Pin Taunhfy hL hunchional geuppsisen thegien
eganic Cempeund.
Physical smte Liauid
Celeu coleuslus
Odeua
Selubiliby Sluble in wl

gbsehvaien
Sno Expesinaant 9bsehMouhien nfaune
AAdd bu imus souutien No change Phunelic and
to he giNen Kaanic Casbozylic acid
Lempeund. gAeuLp absent.

2.Bddad LAAHiC aumeMuM Ne hdLeleuh Heholic geup

nik sutien 2-3 dseps absent.
te the giren Sample).

Hadd 2-3 daeps oh, 2,4-DNP Yelleu h ang abbanmy geup

and chaka usell and alleuotoCyslal smed pausenk
stond

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 2.
Sodiw uke pkuside (SNP) st
CH CocH, +0H ch Co CHT+,0
L Fe CCND
NOJ+CH, COCH [Fe Cc) NO- CH,
NiRophussida ien CoCH,1
Rad celeukatien
 Date

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Expt.No.

Tnfehane
Expesinmat Obsehwahien

auyshallin
pzpptp
.lasbernu.
4Addud2-3ml e sodium gheup Le0isuad
bisulphal to the givern Sampc,
shak Lel Alouoed to stand

Violt celeus Karortc seup

m-inikabenaune lost Tesmd ahichp&LLeNT.
Aadud pin.ch anAaunleh-
M-dinilkebenzen to given eganicsledu dicapp.6
Compound and oddd Imloh
NaOH Sak usdl

Spdiu Nikephussida Test (sNP)

Addud Iml SNP te qin Rd celeuh Ktonic gheup
hgonic_cempsund Shakad Letll LonbMed

and added diI. NaDH_solution

dsepuoise.

Pusudts
h gien .ganic cempeund Lontauns KeTONIC9up
hu

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 Date 27 e1
Expt.No. 1S Page No.

ExPERIMENT15
Pina lo durct h pLLencLo asbehudsali in ha 4ien
esd Cample

Maoals guiad Fond cara.ole o be tstd Melisch

hlagent Fehling's ALagut and Renadict seluion and
Tln s agenti, Conunkald sulphunic acid andh.sodum
hudsoxid seluhon

bsesvadien Tal

Sno Expesinmunt Obeeavanon Tnsene

Chasuing aatien scoubsasbehudsala
Pdaihien eh cenunikaid
Sulphusic acidadldlad duuto dahyclsatiern pMSent.

Lencunkad HSDy dhepueise

tp tha gANen ed Sample.

2. Melisch Rungpnt bt- A uhpe coleus sing Casbehuydkai

Pddud fao daeps e Melischis ennad at h Cenisd
agnt te the 00Aeus Selubion 4unche1 e tue
ogihene0dSample elleted Xaythb
by Cem.einkalud H,SD aleng.
ths sid eh Tt tube..

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 KEACTIONS
Fehling Selution ut
CHO c00 Na

(CHOHD 2 Ga(oH), +NaDH Tastrate (cHOH) +3H,0+ Ga,0

CH,OH CH,OH
Gcsse sod. salE oh
gucenic aci o

ean
oans stugent tst-
Hg ND + NH,DH NH, NO, + HaOH

2 PAgDH g0+H,0
Pa,O+ 2 NH,OH 2 Ag (NH),J OH +3H,0
seluble)

CH,OH COOH

(cHOH+ Ra,0 NH OH CHOHD4 2a

CHO COOH
GlucRse Guconic 0id
 Date

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Expt. No.

Tnfeseno
Sno Exuimunt Bbsesvation

3 Fehling soution tut

Pddad FehRing Selutieru Casbehuyedsat
Racl paacipitalu
Cenpkrmad
AB to the gien sampk
and heald n uals bath

Telon's htagnt st Siny silth Misseasbehyelsati

Pddid 1eons hagpn to Swiny Cen&inmad
fhe gien sampleleused
by feo dseps oh NaDH and
hatid un wal badn fe

Rud paucipitalk laskehudsa

Bendict test
Addd Benadict seuhen COmfanaad
to 4ha oinen eed Sample
ond hsatrd un uoalk
bath
e

Tha qen ee SampleConttins CARBOH YDRATES

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 Date 2711
Expt. No. Page No.

ExpERIMENT-16
Pi le s tha paisenea o phin un fha giened
Sample

Maliials Ruquistd diluit NaDH solution Coppes sulphal.

Seuhion Lenconkafad nilkic acid Ninhydsin suhon od
Somple.and Millen's Aagent

Ahsesvahern labe
Sno Expesinent Bbsesvatie Intnene

Biu t -
Padud uodseps diluti Bppasenca evieit Phcin
NaOH soluhion leused byceieuh Loniswad
SO4 seluhon.

2Nnhydsin tut-
Padud ninhydsin seuthen te Celeu appea Psin náLsent
04uLeUS Geluhen oh teod
Cample and beted

3 XantheNhRia tust- Pddad

o dheps of Conurikatid Yalluo psttipital Piin
HNO tD fh guren sample Lonisn
Ond beil

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 Date
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Page No.

S.no ExpesiAunt Olscwation Tnhune

MiNen tost Ohite phucipitali. Poelain
Addud MillenS Aagcot to Lohich tuhns te had consnmad
tfu ee sample and haaldO
hatin

Pusult
The g n s odsomple
d Contains PROTEIN

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