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    DNA Profiling A.K.A. DNA Fingerprinting Guided Notes What Is DNA Profiling?

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    Jackson Ltorisha
    DNA profiling, also known as DNA fingerprinting, is a technique used by scientists to distinguish between individuals of the same species using only DNA samples. It was developed in the 1980s by Sir Alec Jeffreys who invented the technique. The process involves breaking down DNA samples to release nucleotides, amplifying small amounts of DNA using PCR, cutting the DNA into fragments using restriction enzymes, separating the fragments by size using gel electrophoresis, and analyzing the pattern of fragment distribution. DNA profiling can identify individuals with near certainty except for identical twins.

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    DNA Profiling A.K.A. DNA Fingerprinting Guided Notes What Is DNA Profiling?

    Uploaded by

    Jackson Ltorisha
    48 views2 pages
    DNA profiling, also known as DNA fingerprinting, is a technique used by scientists to distinguish between individuals of the same species using only DNA samples. It was developed in the 1980s by Sir Alec Jeffreys who invented the technique. The process involves breaking down DNA samples to release nucleotides, amplifying small amounts of DNA using PCR, cutting the DNA into fragments using restriction enzymes, separating the fragments by size using gel electrophoresis, and analyzing the pattern of fragment distribution. DNA profiling can identify individuals with near certainty except for identical twins.

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    DNA profiling, also known as DNA fingerprinting, is a technique used by scientists to distinguish between individuals of the same species using only DNA samples. It was developed in the 1980s by Sir Alec Jeffreys who invented the technique. The process involves breaking down DNA samples to release nucleotides, amplifying small amounts of DNA using PCR, cutting the DNA into fragments using restriction enzymes, separating the fragments by size using gel electrophoresis, and analyzing the pattern of fragment distribution. DNA profiling can identify individuals with near certainty except for identical twins.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    48 views2 pages

    DNA Profiling A.K.A. DNA Fingerprinting Guided Notes What Is DNA Profiling?

    Uploaded by

    Jackson Ltorisha
    DNA profiling, also known as DNA fingerprinting, is a technique used by scientists to distinguish between individuals of the same species using only DNA samples. It was developed in the 1980s by Sir Alec Jeffreys who invented the technique. The process involves breaking down DNA samples to release nucleotides, amplifying small amounts of DNA using PCR, cutting the DNA into fragments using restriction enzymes, separating the fragments by size using gel electrophoresis, and analyzing the pattern of fragment distribution. DNA profiling can identify individuals with near certainty except for identical twins.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 2

    DNA Profiling

    a.k.a. DNA Fingerprinting


    Guided Notes

    What is DNA Profiling?


    A ___________________________ used by scientists to distinguish between individuals
    of the same species using only samples of their _______________.

    This is _________________ __________________ who invented this

    technique in the year ____________.

    Stages of DNA Profiling


     Stage 1:
    _______________ are broken down
    to release ________________

    If only a small amount of DNA is available it can be amplified using the


    _________________ ___________________ _________________________ or PCR.

     Step 2:
    The DNA is cut into fragments using __________________ ____________________.

    Each restriction enzyme cuts ______________ at a specific ____________


    ___________________.

    The sections of DNA that are cut out are

    called _______________ ____________________.

    This yields thousands of restriction fragments of all different __________ because the
    base sequences being cut may be ___________ ___________ (long fragment) or
    _____________ _______________ (short fragment).
    Stage 3:
     Fragments are separated on the basis of _________ using a process called
    __________ ______ ___________________.
     DNA fragments are injected into _______________ and an __________________
    current is applied along the gel.

    DNA is _______________ charged so it is attracted to the _________________


    end of the gel.
    The ______________ DNA fragments move ______________ than the longer fragments.
    DNA is separated on basis of ___________.

     A _____________________ material is added which combines with the DNA


    fragments to produce a ____________________ image.
     A photographic ______________ of the DNA bands is obtained.

    Stage 4:
     The pattern of fragment distribution is then analyzed.

    Crime
     The chances of two people having exactly the same
    DNA profile is 30,000 million to 1 (except for
    identical twins).
    Biological materials used for DNA profiling
     Blood
     Hair
     Saliva
     Semen
     Body tissue cells
     DNA samples have been obtained from vaginal cells
    transferred to the outside of a condom during sexual
    intercourse.

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