5989 8442en
5989 8442en
5989 8442en
Application
Food
2
LC Conditions
Column: Inertsil ODS3, 250 mm × 2.1 mm, 5 µm
Mobile phase: A = 1% formic acid
B = acetonitrile
Gradient: Start with 5% B
At 30 min 50% B
Flow rate: 0.2 mL/min
Column temperature: 40 °C
Injection volume: 10 µL
MS Conditions
Source: ESI
Ion mode: Positive
Vcap voltage: 4000 V
Nebulizer: 50 psig
Drying gas flow: 10 L/min
Drying gas temp: 350 °C
Corona: 4 µA
Vaporizer temperature: 350 °C
Scan range: 100–1200 amu
Step size: 0.1
Peak width: 0.15 min
Time filter: On
Fragmentor: 200 V
Monascus Colorant
3
Figure 4. The major pigments of Monascus colorant.
LC Conditions
Column: Inertsil ODS3, 250 mm × 2.1 mm, 5 µm
Mobile phase: A = 1% formic acid
B = acetonitrile
Gradient: Start with 50% B
At 10 min 90% B
Flow rate: 0.2 mL/min
Column temperature: 40 °C
Injection volume: 10 µL
MS Conditions
Source: ESI
Ion mode: Positive
Vcap voltage: 4000 V
Nebulizer: 50 psig
Drying gas flow: 10 L/min
Drying gas temp: 350 °C
Corona: 4 µA
Vaporizer temperature: 350 °C
Scan range: 100–1200 amu
Step size: 0.1
Peak width: 0.15 min
Time filter: On
Figure 5. The total ion chromatogram of Monascus colorant. Fragmentor: 100 V
4
Figure 6. Mass spectra of major pigments in Monascus colorant.
Three major peaks with base peaks at m/z 439, The protonated molecular ions [M+H]+ were
467, and 495 were not identified. Figure 6 shows observed for every major pigment. See
the mass spectra of the identified pigments. Proto- Figure 9. However, with the exception of capsan-
nated molecular ions [M+H]+ were observed for the thin monoeicosanoate, the intensity of these ions
four identified pigments. was very low. Except for capsanthin monoeico-
sanoate, the pigments show fragment ions result-
Paprika Color ing from the loss of one or two fatty acid
fragments. A common fragment ion was observed
Capsanthin and the mono- and di- esters of cap- at m/z 567 in the mass spectra of these pigments.
santhin with fatty acids are known as the major
pigments in paprika colorant. See Figure 7. Two
monoesters and five diesters of capsanthin were
identified in the paprika colorant analyzed in this
study. See Figure 8.
5
Figure 7. The structure of major pigments of paprika colorant.
6
LC Conditions
Column: Inertsil ODS3, 250 mm × 2.1 mm, 5 µm
Mobile phase: A = acetone
B = methanol
Gradient: Start with 10% B
At 10 min 90% B
Flow rate: 0.2 mL/min
Column temperature: 40 °C
Injection volume: 10 µL
MS Conditions
Source: APCI
Ion mode: Positive
Vcap voltage: 4000 V
Nebulizer: 50 psig
Drying gas flow: 5 L/min
Drying gas temp: 350 °C
Corona: 4 µA
Vaporizer temperature: 350 °C
Scan range: 100–1200 amu
Step size: 0.1
Peak width: 0.15 min
Time filter: On
Lac Colorant
7
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Figure 10. The structure of major pigments of lac colorant. Figure 11. The total ion chromatogram of lac colorant.
LC Conditions
Column: Inertsil ODS3, 250 mm × 2.1 mm, 5 µm
Mobile phase: 30% acetonitrile in 5 mM dibutylamine,
isocratic
Flow rate: 0.2 mL/min
Column temperature: 40 °C
Injection volume: 10 µL
MS Conditions
Source: ESI
Ion mode: Negative
Vcap voltage: 4000 V
Nebulizer: 50 psig
Drying gas flow: 10 L/min
Drying gas temp: 350 °C
Scan range: 100–1200 amu
Step size: 0.1
Peak width: 0.15 min
Time filter: On
Fragmentor: 100 V
Conclusions
Four commercial natural colorants were analyzed
using ESI and APCI-LC/MS. The MS data provided
molecular weight information and some structural Agilent shall not be liable for errors contained herein or for incidental or consequential
damages in connection with the furnishing, performance, or use of this material.
information for the major pigments
Information, descriptions, and specifications in this publication are subject to change
without notice.