Scribd Logo
Upload

Welcome to Scribd!

  • 21 views

    I Applic COTININE

    Uploaded by

    LyteC123
    The document defines test parameters for measuring cotinine levels in samples using photometric methods. It provides details on calibration, test limits, sample types, reagents, and quality control samples. The test uses two reagents in a multi-step process to measure absorbance at 340nm and calculate cotinine concentration.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    I Applic COTININE

    Uploaded by

    LyteC123
    21 views1 page
    The document defines test parameters for measuring cotinine levels in samples using photometric methods. It provides details on calibration, test limits, sample types, reagents, and quality control samples. The test uses two reagents in a multi-step process to measure absorbance at 340nm and calculate cotinine concentration.

    Original Title

    841111_I_applic_COTININE

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document defines test parameters for measuring cotinine levels in samples using photometric methods. It provides details on calibration, test limits, sample types, reagents, and quality control samples. The test uses two reagents in a multi-step process to measure absorbance at 340nm and calculate cotinine concentration.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    21 views1 page

    I Applic COTININE

    Uploaded by

    LyteC123
    The document defines test parameters for measuring cotinine levels in samples using photometric methods. It provides details on calibration, test limits, sample types, reagents, and quality control samples. The test uses two reagents in a multi-step process to measure absorbance at 340nm and calculate cotinine concentration.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Document number: 841111_I_applic_COTININE, Date: 02/04, s.w. 6.

    0
    Test definition COTININE Calibr. parameters
    Test type Photometric Test in use YES Calibration type NONLINEAR
    Full name COTININE Low High Units Bias corr. in use NO
    Online name Test limit 0 2000 µg/l Repeat time (d) 3
    Result Unit µg/l low( Ö ), high (!) Abs. error (mA) 5
    Point/Calibrator Single
    Number of decimals 0 Initial absorbance 0 2 A Rel. error (%) 0
    Acceptance MANUAL
    Acceptance AUTOMATIC Response limit
    (mA)
    Type of calibrator SEPARATE Min. *
    Dilution 1 + 0 Dilution limit (!) * * µg/l Calibrator Conc.(!) Dil. Ratio. 1+ Max. *
    Sample type DoA Cal D0 0 0
    Secondary dil. 1+ 0 0 DoA Cal D1 100 0
    ¨Serum ¨Plasma ýUrine DoA Cal D2 250 0
    ¨C.S.F ¨Other Correction factor 1 DoA Cal D3 500 0
    More DoA Cal D4 1000 0
    Correction bias 0 DoA Cal D5 2000 0

    Test flow Reagent definition


    Blank NO Fixed cuvette
    Antigen excess NO

    Syringe BCR Id. Stable


    speed on board
    (days)
    Name
    Curve type
    Incubation Incubation
    Reagent Volume (µl) Time (sec.) Reagent Time (sec.) λ 1 (nm) NONLINEAR
    COTIN1 9 300 COTIN2 90 340 Nonlinearity
    Conc.(µg/l)

    Volume (µl) Disp. with Volume (µl) λ 2 (nm) 80 COTIN1 Slow 413 30
    55 WATER 55 NONE %

    Disp. with Volume (µl) Disp. with 20 COTIN2 Slow 414 30


    Extra with 10 Extra with
    buffers buffers Time (sec)
    180
    Volume (µl) Dilution with Volume (µl)
    8 WATER 8
    Points & Inter.

    Wash reagent Wash reagent Wash reagent Konelab 6/36


    60/30/20XT
    NONE [WATER] NONE

    Konelab™ COTININE - code: 981632 (2 x 30 ml)

    Reagent: Liquids, ready-to-use. It is recommended to store the reagents tightly sealed at 2...8 °C when not in use.
    Calibrator: DoA Calibration set D, 981633. Liquids, ready to use.

    Q.C. sample: DoA Control set D, 981735. Liquids, ready to use.

    You might also like