Bioenergetics Handout

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Bioenergetics is 

the branch of biochemistry that focuses on how cells transform energy, often by producing, storing or Bioenergetics is the branch of biochemistry that focuses on how cells transform energy, often by producing, storing or
consuming adenosine triphosphate (ATP). consuming adenosine triphosphate (ATP).

CELLULAR RESPIRATION is a process of releasing energy stored in sugar in the presence of oxygen. Cellular CELLULAR RESPIRATION is a process of releasing energy stored in sugar in the presence of oxygen. Cellular
respiration includes both aerobic and anaerobic but is often used to refer to aerobic respiration. respiration includes both aerobic and anaerobic but is often used to refer to aerobic respiration.
 AEROBIC RESPIRATION consumed organic molecules and O2 and yields ATP.  AEROBIC RESPIRATION consumed organic molecules and O2 and yields ATP.
 Anaerobic respiration is similar to aerobic respiration but consumes compounds other than O2.  Anaerobic respiration is similar to aerobic respiration but consumes compounds other than O2.

EQUATION OF CELLULAR RESPIRATION EQUATION OF CELLULAR RESPIRATION

NOTE: During cellular respiration, NOTE: During cellular respiration,


the fuel (such as glucose (C6H12O6) is oxidized, and O2 is reduced. the fuel (such as glucose (C6H12O6) is oxidized, and O2 is reduced.
 Oxidation - loses electron  Oxidation - loses electron
 Reduction - gains electron  Reduction - gains electron
THE STAGES OF CELLULAR RESPIRATION THE STAGES OF CELLULAR RESPIRATION
Harvesting of energy from glucose has three stages: Harvesting of energy from glucose has three stages:

1. Glycolysis is the breakdown of glucose to pyruvate where small amounts of ATP are produced. This 4. Glycolysis is the breakdown of glucose to pyruvate where small amounts of ATP are produced. This
process occurs in the cytoplasm of the cell.
process occurs in the cytoplasm of the cell.
5. The Citric Acid Cycle or Krebs Cycle degrades pyruvate to carbon dioxide, water, ATP and reducing
2. The Citric Acid Cycle or Krebs Cycle degrades pyruvate to carbon dioxide, water, ATP and reducing
power in the form of NADH, H+. This stage happens in the matrix of the mitochondria.
power in the form of NADH, H+. This stage happens in the matrix of the mitochondria.
6. Oxidative Phosphorylation which includes electron transport chain and chemiosmosis generates high
3. Oxidative Phosphorylation which includes electron transport chain and chemiosmosis generates high
amount of ATP, because it is powered by redox reaction. This stage occurs in the inner membrane of the
amount of ATP, because it is powered by redox reaction. This stage occurs in the inner membrane of the
mitochondria.
mitochondria.

TYPES OF FERMENTATION
TYPES OF FERMENTATION
 Fermentation consists of glycolysis plus reactions that regenerate NAD+, which can be reused by glycolysis.
 Fermentation consists of glycolysis plus reactions that regenerate NAD+, which can be reused by glycolysis.
Two Common Types are:
Two Common Types are:
1. ALCOHOL FERMENTATION – pyruvate is converted to ethanol in two steps, with the first
1. ALCOHOL FERMENTATION – pyruvate is converted to ethanol in two steps, with the first
releasing CO2.
releasing CO2.
 alcohol fermentation by yeast us used in brewing, winemaking, and baking.
 alcohol fermentation by yeast us used in brewing, winemaking, and baking.
2. LACTIC ACID FERMENTATION – pyruvate is reduced to NADH, forming lactate as an end product, with no
2. LACTIC ACID FERMENTATION – pyruvate is reduced to NADH, forming lactate as an end product, with no
releasing of CO2.
releasing of CO2.
 lactic acid fermentation by some fungi and bacteria is used to make cheese and yogurt.
 lactic acid fermentation by some fungi and bacteria is used to make cheese and yogurt.
 Human muscle cells use lactic acid fermentation to generate ATP when O2 is scarce.
 Human muscle cells use lactic acid fermentation to generate ATP when O2 is scarce.

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