Scribd Logo
Upload

Welcome to Scribd!

  • 40 views

    Lab Report Bio Exp2

    Uploaded by

    NURIN ALIS BINTI FADZIL Moe
    This experiment aims to prepare a wet mounted slide of onion epidermal tissue and observe the cells under a microscope. Procedures involve staining a strip of onion epidermis with iodine solution and viewing under 10x and 40x objectives. Observations found rectangular shaped cells due to their cell walls. Starch granules within were stained brown by iodine. Onion epidermal cells lack chloroplasts as they receive no light for photosynthesis. The cells are compared to mesophyll and xylem cells as well as bacterial and epithelial cells based on their structures and organelles.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Lab Report Bio Exp2

    Uploaded by

    NURIN ALIS BINTI FADZIL Moe
    40 views3 pages
    This experiment aims to prepare a wet mounted slide of onion epidermal tissue and observe the cells under a microscope. Procedures involve staining a strip of onion epidermis with iodine solution and viewing under 10x and 40x objectives. Observations found rectangular shaped cells due to their cell walls. Starch granules within were stained brown by iodine. Onion epidermal cells lack chloroplasts as they receive no light for photosynthesis. The cells are compared to mesophyll and xylem cells as well as bacterial and epithelial cells based on their structures and organelles.

    Original Title

    LAB REPORT BIO EXP2

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This experiment aims to prepare a wet mounted slide of onion epidermal tissue and observe the cells under a microscope. Procedures involve staining a strip of onion epidermis with iodine solution and viewing under 10x and 40x objectives. Observations found rectangular shaped cells due to their cell walls. Starch granules within were stained brown by iodine. Onion epidermal cells lack chloroplasts as they receive no light for photosynthesis. The cells are compared to mesophyll and xylem cells as well as bacterial and epithelial cells based on their structures and organelles.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    40 views3 pages

    Lab Report Bio Exp2

    Uploaded by

    NURIN ALIS BINTI FADZIL Moe
    This experiment aims to prepare a wet mounted slide of onion epidermal tissue and observe the cells under a microscope. Procedures involve staining a strip of onion epidermis with iodine solution and viewing under 10x and 40x objectives. Observations found rectangular shaped cells due to their cell walls. Starch granules within were stained brown by iodine. Onion epidermal cells lack chloroplasts as they receive no light for photosynthesis. The cells are compared to mesophyll and xylem cells as well as bacterial and epithelial cells based on their structures and organelles.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 3

    Tittle: Experiment 2: Staining Onion Epidermal Tissue

    Objectives
    i. To prepare wet mounted slide.
    ii. To draw and identify the onion epidermal cell.

    Introduction

    The characteristic of living plant cell have cell wall, large central
    vacuole and chloroplast. Next, the type of tissue for onion
    epidermal cell is parenchyma. However, the function of onion
    Procedures epidermal cell are chlorophyl and chloroplast responsible for
    photosynthesis. The layers of onion contain simple sugars
    (carbohydrates) some of which are stored as starch (starch
    granules).

    1. One drop of diluted iodine was put on the slide.


    2. The inner epidermal layer from the scale of an onion was
    stripped off and cut it into smaller pieces.
    3. The onion epidermal layer was place on the slide. Make
    sure the tissue was not folded.
    4. One drop of diluted iodine solution was applied on the
    onion epidermal tissue.
    5. A cover slip was used to lower the cover slip gently.
    6. The excessive iodine was remove using a filter paper.
    7. The tissue was observed using 10x or 40x objective lens.
    8. Observation at high magnification was drew.

    Observation
    Discussion

    The function of iodine is to stain the starch. Besides, the shape of onion epidermal
    cell is rectangular shape due to present of cell wall and the position of its nucleus
    at peripheral of cell due to large central vacuole. Next, the arrangement of onion
    epidermal cell is rectangular in shape, compactly arranged and without any
    intercellular spaces. Furthermore, organelle is not visible in the cell under the light
    microscope is chloroplast. It is because onion cell gets no light to carry out
    photosynthesis and largely present in the leafy part on the onion, which is exposed
    to the sun.

    Comparison the onion epidermal cell with two other plant cells, mesophyll cell and
    xylem based on the structures and organelles present:

    Epidermal cell Mesophyll cell Xylem


    Similarity All in a leaf structure
    Structures Has cell wall Have thin and Has thick
    flexible lignified
    primary cell secondary
    walls cell wall
    Has a large Have a large Elongated
    vacuole central and long tube
    vacuole
    Organelles Large Chloroplast, No organelle
    vacuole, mitochondria,
    mitochondria, nuclei,
    Golgi body, smooth
    smooth endoplasmic
    endoplasmic reticulum and
    reticulum and rough
    rough endoplasmic
    endoplasmic reticulum
    reticulum

    Comparison the eukaryotic ’s epidermal cell with prokaryotic ’s bacterial cell based
    on structures:
    Epidermal cell Bacterial cell
    Similarity Both have ribosome
    Differences Larger in size Smaller in size
    DNA contains in the DNA lies freely in the
    nucleus cytoplasm
    Comparison the epidermal cell with epithelial cell based on the structure:

    Epidermal cell Bacterial cell


    Similarity Both are in single layer
    References

    Campbell, N.A, Urray. L.A, Gain, M.L, Wasserman, S.A.,


    Minorsky, P.V. & Orr, R.B. (2021) Biology (12thed).
    Pearson Education

    Byju’ s Raveendran and Divya Gokulnath. (2011). “The shape of


    onion peel cells taken for observation under the microscope is”: Byju’ s
    https://byjus.com/question-answer/the-shape-of-onion-peel-cells-taken-for-
    observation-under-the-microscope-is-roundrectangularspindleelliptical/

    You might also like