Scribd Logo
Upload

Welcome to Scribd!

  • 62 views

    Matlab Patch Clamp Technique

    Uploaded by

    Rafael Hernández Mendez
    The patch clamp technique allows the study of single or multiple ion channels in cells by using a glass micropipette electrode that forms a high resistance seal with the cell membrane. This seal electrically isolates currents across the membrane patch and allows researchers to observe changes in voltage or current. The patch clamp technique is commonly used in electrophysiology to study the electrical properties and action potentials of neurons and other excitable cells.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Matlab Patch Clamp Technique

    Uploaded by

    Rafael Hernández Mendez
    62 views3 pages
    The patch clamp technique allows the study of single or multiple ion channels in cells by using a glass micropipette electrode that forms a high resistance seal with the cell membrane. This seal electrically isolates currents across the membrane patch and allows researchers to observe changes in voltage or current. The patch clamp technique is commonly used in electrophysiology to study the electrical properties and action potentials of neurons and other excitable cells.

    Original Description:

    Documentación de Matlab

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The patch clamp technique allows the study of single or multiple ion channels in cells by using a glass micropipette electrode that forms a high resistance seal with the cell membrane. This seal electrically isolates currents across the membrane patch and allows researchers to observe changes in voltage or current. The patch clamp technique is commonly used in electrophysiology to study the electrical properties and action potentials of neurons and other excitable cells.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    62 views3 pages

    Matlab Patch Clamp Technique

    Uploaded by

    Rafael Hernández Mendez
    The patch clamp technique allows the study of single or multiple ion channels in cells by using a glass micropipette electrode that forms a high resistance seal with the cell membrane. This seal electrically isolates currents across the membrane patch and allows researchers to observe changes in voltage or current. The patch clamp technique is commonly used in electrophysiology to study the electrical properties and action potentials of neurons and other excitable cells.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    You are on page 1of 3

    About Patch Clamp Technique

    Example document for demo purposes based on excerpts from relevant Wikipedia articles:
    http://en.wikipedia.org/wiki/Patch_clamp, http://en.wikipedia.org/wiki/Electrophysiology

    The patch clamp technique is a laboratory technique in electrophysiology that allows the study
    of single or multiple ion channels in cells. The technique can be applied to a wide variety of
    cells, but is especially useful in the study of excitable cells such as neurons.

    Electrophysiology

    Electrophysiology is the study of the electrical properties of biological cells and tissues. It includes
    measurements of the electrical activity of neurons, and particularly action potential activity.

    "Current Clamp" is a common technique in electrophysiology. This is a whole-cell current clamp


    recording of a neuron firing due to it being depolarized by current injection

    Basic technique

    Patch clamp recording uses, as an electrode, a glass micropipette that has an open tip diameter of
    about one micrometer, a size enclosing a membrane surface area or "patch" that often contains
    just one or a few ion channel molecules. This type of electrode is distinct from the "sharp
    microelectrode" used to impale cells in traditional intracellular recordings, in that it is sealed
    onto the surface of the cell membrane, rather than inserted through it. In some experiments, the
    micropipette tip is heated in a microforge to produce a smooth surface that assists in forming a
    high resistance seal with the cell membrane. The interior of the pipette is filled with a solution
    matching the ionic composition of the bath solution, as in the case of cell-attached recording, or
    the cytoplasm for whole-cell recording. A chlorided silver wire is placed in contact with this
    solution and conducts electric current to the amplifier. The investigator can change the
    composition of this solution or add drugs to study the ion channels under different conditions.
    The micropipette is pressed against a cell membrane and suction is applied to assist in the
    formation of a high resistance seal between the glass and the cell membrane. The high resistance
    of this seal makes it possible to electronically isolate the currents measured across the membrane
    patch with little competing noise, as well as providing some mechanical stability to the
    recording.

    Unlike traditional two-electrode voltage clamp recordings, patch clamp recording uses a single
    electrode to record currents. Many patch clamp amplifiers do not use true voltage clamp circuitry
    but instead are differential amplifiers that use the bath electrode to set the zero current level. This
    allows a researcher to keep the voltage constant while observing changes in current.

    Alternatively, the cell can be current clamped in whole-cell mode, keeping current constant
    while observing changes in membrane voltage .
    Electrical diagram

    From Stefan Mergler’s page http://patch-clamp.info/techniques/techniques.htm

    Simplified diagram of a patch-clamp amplifier and a substitute circuit of a whole-cell


    configuration : Rf: feedback resistor, Vcom: command voltage, Vpip: pipette potential, Vout:
    output voltage proportional to the current. The circuit of the amplifier represents a so-called
    current-to-voltage converter, which is installed in a little box (preamplifier) near the pipette.
    After breaking through the membrane, the whole-cell configuration is obtained. Thereby,
    membrane fragments and other cell debris are sucked near the pipette opening and could
    subsequently increase the serial resistance RS.

    See Also: Scholarpedia article on dynamic properties of cell membranes:


    http://www.scholarpedia.org/article/Electrical_properties_of_cell_membranes#Membrane_patch:
    _temporal_dynamics

    You might also like