Novicio, Hannah

S11-11

Pineapple Wine Fermentation

I. Introduction
Actual: Ola! I am Hannah Novicio, your experimenter for today! Fun fact, the fruit that
we are going to experiment with today came from the country of South Brazil. Hmm, you
know what it's all about? It is a greenish-yellow fruit. Hmm, another clue? That's what
you're calling, too many eyes. What?! What? Yes, you're right, this is a pineapple!

- Did you know that in making wines, pineapples are also used? Typically,
wines are made from grapes, making pineapple wines extraordinary.
Pineapple wines are alcoholic beverages made with fermentation process
pineapples.

- Wait, what? A process of fermentation of pineapples? I think you seldom hear

that, right? So, if you'd like to know more about it, come and join me! As we
study how ethanol fermentation takes place in the process of making
pineapple wine, we'll be an experimenter slash investigator for today.

- But wait, let me share my knowledge with you. What is fermentation? To create
substances that can be used in the processing of chemical energy, fermentation is the
breaking down of sugar molecules into simpler compounds. It is a chemical mechanism
in which molecules such as glucose are anaerobically broken down. That is to say,
fermentation is an anaerobic process in which, while oxygen is not available, energy
can be released from glucose.
Voiceover: Did you know that the term "ferment" comes from the Latin word fervere, which
means "to boil?" Zymology is called the analysis of fermentation and its practical uses and
originated in 1856 when the French chemist Louis Pasteur showed that yeast induced
fermentation. In some forms of bacteria and fungi that need an oxygen-free environment to
survive in optional anaerobes such as yeast, fermentation occurs, as well as in muscle cells when
oxygen is in short supply.

- For the food and beverage industries, fermentation processes are valuable, with
the conversion of sugars into ethanol used for the manufacture of alcoholic drinks, the
release of CO2 by the yeast used for the touch of sweetness of the bread, and the
production of organic acids for the preservation and flavoring of dairy products, and also
vegetables.
 - There are two types of fermentation: ethanol fermentation and lactic acid fermentation,
but in these episodes, we will focus only on the first type, ethanol fermentation.

II. Process Making

Actual: Haha, sorry if I’m talking to much. But now, we’re going to begin the experiment that
you’ve been waiting for! As I said a while ago, we will tackle the process of fermentation of
ethanol by making pineapple wine.

- But first let’s remember the following safety precautions to avoid accidents before,
during, and after the experiment: wear safety gears such as an apron and follow the
procedure carefully.

- And also, I'm going to tie my hair to avoid accidentally dip it into the chemicals I'm
working with.

- //transition// Woop! Welcome to my humble kitchen, let me incorporate the following

ingredients that are needed to perform the experiment.

● - 1 ripe pineapple fruit

● - 1/4 cup sugar – 4 table spoon
● - 1 clean bottle
● - 1/4 tablespoon yeast
● - 1 rubber balloon
● - Clean containers
● - Clean piece cloth
● - Stove
● - Knife
● - Casserole
- Ok, so I think we’re ready for the experimentation. So.. Let’s start!
Voiceover: Step by step Procedure
1. Peel the pineapple including the eyes. Remove the hard core.
2. Cut into small pieces. Extract the juice by squeezing the fruit using a clean piece of cloth.

3. Heat the juice and let it simmer for 10 minutes. Allow to cool then strain the juice using a
clean piece of cloth.

4. Mix a cup of sugar for every liter of pineapple juice prepared. Transfer the juice in a
clean glass bottle. Add yeast.

5. Cover the bottle using a clean rubber balloon. Secure the balloon with a rubber band.

6. Set it aside in a cool dry place for five to seven days. Stir daily.
 7. For best results, strain the mixture and place in a clean bottle then store it for at least two
months.

- Wow, we have just done the procedures to ferment pineapple wine! We are
halfway through this because we are going to wait five days to see the result. I’ll be
back, see you!

III. Final Result

Actual: Hi, welcome back! How’s your day? Do you want to know the result of the
experiment? Here you go..

- Ethanol Fermentation, also known as alcohol fermentation, is a biological

process that converts into cellular energy sugars such as glucose, fructose,
and sucrose, producing as by-products ethanol and carbon dioxide.

Voiceover: In the experiment, this process was mainly due to yeast, which became the
key for fermentation as it absorbs or consumes sugar (glucose) and simultaneously
releases carbon dioxide. Yeast is a single cell known as fungi and so functional as it
unleashes the desired texture, color, and smell of bread and pastries, especially in the
baking field.

Actual: Okay, since I’m happy to share that I made this experiment with you. Let me
share with you the fact that I gathered from the internet. As described earlier, it can be
taken as a type of vitamin supplement, since yeast is very multipurpose. It is found that
this unicellular fungus or microorganism is rich in nutrients such as vitamin B, folic acid,
niacin and a lot of protein. It is also, in addition, a consideration used in the field of
genetics. In experiments and studies concerning the genetics and life cycles of different
species, these microorganisms are widely used.

Voiceover: Now, referring to the experiment, the yeast will turn the sugar into alcohol as
it tears or breaks it down in the process, though oxygen is not present. Phosphates are
added to the sugar following the introduction of active yeasts to the pineapple must, and
the six-carbon sugar molecules begin to be divided into three-carbon pieces and go
through a series of rearrangement reactions. The carboxyl carbon atom is released in
the form of carbon dioxide during this process, with the remaining components being
acetaldehyde. In this anaerobic process, the absence of oxygen enables the
acetaldehyde to be gradually converted to ethanol by reduction. Subsequently, if more
sugars are carried out, more bubbles or pressure will also increase. Since the path of
the carbon is blocked by the balloon, it won't be released. Hence, days after setting
aside, the balloon would be packed with carbon or air within it. The longer the time span
has been set aside, the larger and inflated the balloon would be.

IV. Applications of Fermentation

Actual: Let’s now move on to the one of the interesting parts of fermentation. Let’s now
move to the applications of it. Back when our ancestors had no resources to provide
refrigerators and coolers, they used the fermentation method that required only yeast or
salt. Practically speaking, the facilities around them were only used and rendered for
convenience. Let me tell you about some of the goods or food, when the yeast is a
building block.

Voiceover: First of all, in the United States, popcorn seasoning is mass-produced for
commercial and consumer use by many businesses. To boost the taste of popcorn,
popcorn seasonings may be used, and others are used to give a buttery flavor to
popcorn. In Atlanta, the Plaza Theatre provides nutritional yeast for popcorn seasoning
for vegan tourists.

- And not least, the thickened soups are blended with nutritional yeast. This is a
deactivated yeast, mostly a Saccharomyces cerevisiae strain. In color, it is
yellow and comes in a flake or powdered shape. It is widely used as a
substitute for cheese by vegetarians, as it gives off a nutty, cheesy, creamy
flavor. It can not only be mixed into soups, but it was also delicious with
nutritional yeast.

Actual: Okay, so I think the end of this journey. I’m so grateful that I shared to you my
knowledge about this topic. And I also hope that you’ve learned something new today.
Thank you for listening! Chao!

V. Citation

- 14 fun facts about Hawaiian Pineapples (origin, history, pizza Hawaii, ). (2020,
June 17). Retrieved from
https://www.lovebigisland.com/quick-and-remarkable-facts-
 about-hawaii/pineapple/#:~:text=Pineapples come originally from
South,Guadeloupe in 1493 [1]

- Fermentation in winemaking. (2021, January 05). Retrieved from

https://en.wikipedia.org/wiki/Fermentation_in_winemaking

- Cookist. (2020, July 31). Everything You Need To Know About Pineapple
Wine In A Jiffy! Retrieved from
https://www.cookist.com/everything-you-need-to-
know-about-pineapple-wine-in-a-jiffy/

- Luo, L. (2020, March 01). 5 Uses of Fermentation. Retrieved from

https://sciencing.com/5-uses-fermentation-10015326.html