Benchtop UV Transilluminator

Operating Instructions

IMPORTANT: READ THESE INSTRUCTIONS BEFORE OPERATING YOUR UVP

TRANSILLUMINATOR TO FAMILIARIZE YOURSELF WITH ITS OPERATION.

UVP, LLC 2066 W 11th Street, Upland, CA Ultra-Violet Products Ltd., Trinity Hall Farm Estate,
Tel: (800) 452-6788 / (909) 946-3197 Unit 1, Nuffield Road, Cambridge CB4 1TG UK
Fax: (909) 946-3597 Tel: +44(0)1223-420022 / Fax: +44(0)1223-420561

Web Site: www.uvp.com

81-0118-01 Rev D
 CONTENTS

Introduction .......................................................................................................................... 1

Important Safety Information ................................................................................................. 1

Description/Specifications ..................................................................................................... 1
Single UV Benchtop Transilluminator
2UV Benchtop Transilluminators
3UV Benchtop Transilluminators

Ultraviolet Spectral Output Charts ......................................................................................... 3

Transilluminator Operation ..................................................................................................... 3

Single UV Benchtop Transilluminators
2UV Benchtop Transilluminators
3UV Benchtop Transilluminators

Maintenance/Repair/Technical Assistance ............................................................................ 4

Single UV Transilluminator Schematic
2UV Transilluminator Schematic
3UV Transilluminator Schematic

Replacing the UV Tubes ........................................................................................................ 7

In the Single UV Benchtop Transilluminator
In the 2UV Benchtop Transilluminators
In the 3UV Benchtop Transilluminators

Cleaning the Transilluminator ................................................................................................. 7

Replacement Parts and Accessories ..................................................................................... 8

Warranty .............................................................................................................................. 9

UVP ... Providing quality products for the researcher since 1932 ......................................... 9
Benchtop UV Transilluminators Page 1

Introduction

The UVP Benchtop Transilluminator Series offers the researcher uniform and intense sources of ultraviolet
light (radiation) in a compact package. Their special design emits either one, two or three high intensity
excitation UV wavelengths for back-illumination of transparent fluorescent materials.

The 302nm UV back-illumination included in all Benchtop Transilluminators provides a highly sensitive
method to detect double-stranded nucleic acids that have been labeled with fluorescent dyes such as
ethidium bromide or acridine orange. Single stranded nucleic acids may be detected, but with a lower
excitation wavelength more sensitive for nucleic acid visualization than the 365nm model.

NOTE: Though UVP refers to the mid-range UV waveband as 302nm, others refer to this region as 300nm
or 312nm. The spectral output of all these regions is the same.

Important Safety Information

A Word of Caution: All UVP Benchtop Transilluminators are powerful sources of UV radiation that will cause
damage to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area are properly
protected. It is preferable that the transilluminator be installed and operated in a darkroom where access and
exposure is limited while the unit is in operation. If a darkroom is unavailable, UVP offers a Darkroom which
provides protection from accidental exposure. For part numbers of the Darkroom, contact UVP. Each transillu-
minator is shipped a UV Blocking Cover. Even though this cover blocks the ultraviolet radiation emitted by the
unit, UV Blocking Eyewear should be worn as well. UVP has a complete line of UV Blocking Eyewear:
Spectacles, Goggles, and Faceshield designed for this purpose. For part numbers, see Section 9.

Description/Specifications

Single UV Benchtop Transilluminator

The dimensions of the Single UV Benchtop Transilluminator models are:

Width: 13.25” (33.7cm) / Depth: 9.50” (24.1cm) / Height: 4.75” (12.1cm)

Nominal
Model Part Number Volt/Hz/Amp Wavelength Filter Size # of Tubes Intensity Style

M-15E 95-0218-01 115/60/0.7 302nm 15 x 15cm 5 x 8W Single

95-0218-02 230/50/0.6
95-0218-03 100/50-60/0.8

M-15 95-0219-01 115/60/0.7 302nm 15 x 15cm 5 x 8W Hi/Lo

95-0219-02 230/50/0.6
95-0219-03 100/50-60/0.8

M-20E 95-0210-01 115/60/0.7 302nm 20 x 20cm 6 x 8W Single

95-0210-02 230/50/0.6
95-0210-03 100/50-60/0.8

M-20 95-0216-01 115/60/0.7 302nm 20 x 20cm 6 x 8W Hi/Lo

95-0216-02 230/50/0.6
95-0216-03 100/50-60/0.8

M-26E 95-0221-01 115/60/0.7 302nm 21 x 26cm 6 x 8W Single

95-0221-02 230/50/0.6
95-0221-03 100/50-60/0.8

M-26 95-0222-01 115/60/0.7 302nm 21 x 26cm 6 x 8W Hi/Lo

95-0222-02
95-0222-03
Benchtop UV Transilluminators Page 2

2UV Benchtop Transilluminator

The physical dimensions of all models are:

Width: 13.25” (33.7cm)

Depth: 9.50” (24.1cm)
Height: 4.75” (12.1cm)

Nominal
Model Part Number Volt/Hz/Amp Wave-Length Filter Size #of Tubes/ Wavelength

LM-20E 95-0212-01 115/60/0.7 302nm/365nm 20 x 20cm 6 x 8W/302nm 5 x 8W/365nm

95-0212-02 230/50/0.6
95-0212-03 100/50-60/0.8

LM-26E 95-0223-01 115/60/0.7 302nm/365nm 21 x 26cm 6 x 8W/302nm 5 x 8W/365nm

95-0223-02 230/50/0.6
95-0223-03 100/50-60/0.8

3UV Benchtop Transilluminator

The physical dimensions of all models are:

Width: 14.0” (35.6cm)

Depth: 11.0” (27.9cm)
Height: 5.4” (13.7cm)

Nominal
Model Part Number Volt/Hz/Amp Wavelength Filter Size #of Tubes/ Wavelength

LMS-20E 95-0220-01 115/60/0.85 302/365/254nm 20 x 20cm 6 x 8W/302nm

LMS-20E 95-0220-02 230/50/0.75 5 x 8W/365nm
LMS-20E 95-0220-03 100/50-60/0.90 5 x 8W/254nm

LMS-26E 95-224-01 115/60/0.85 302/365/254nm 21 x 26cm 6 x 8W/302nm

LMS-26E 95-224-02 230/50/0.75 5 x 8W/365nm
LMS-26E 95-224-03 100/50-60/0.90 5 x 8W/254nm
Benchtop UV Transilluminators Page 3

Ultraviolet Spectral Output Charts

Transilluminator Operation

Single UV Benchtop Transilluminator

Place the transilluminator on a level work surface. Be sure that an air space exists around the bottom of the
work surface. This space allows for the proper air circulation through the unit.

Plug the female end of the power cord into the transilluminator. For 230 volt models, or those requiring
special power cord connectors, ensure that the proper configuration of male connector or plug has been
properly connected to the power cord.

Plug the male end of the power cord into a properly grounded electrical outlet. The proper voltage of the
transilluminator is found on the product information label.

The transilluminator comes equipped with a UV Blocking Cover. If this cover is not in place or has been
removed, do not operate the unit without securing the cover. If the cover is missing, a UV Blocking Faceshield
must be worn to avoid UV exposure to the skin. UV Blocking Eyewear should be worn even with the cover
in place to avoid accidental UV exposure.
Benchtop UV Transilluminators Page 4

Place gel/sample on the filter area. It is recommended to place the gels on a UVP Gel-Tray (see Section 10
for part number) to protects filter surface from cuts and scratches. It is recommended that gloves be worn
to avoid contact with gel and staining agents.

Press the ON/OFF switch to ON. The UV tubes within the unit should be glowing beneath the filter after a
momentary flickering during the start-up period.

After viewing/photographing the sample, turn the unit off.

Clean unit surface with a damp soft cloth or sponge. Never use abrasive cleaners (can damage the UV
filter surface).

If you have any questions, call UVP Customer Service toll free in the U.S. at (800) 452-6788 or (909) 946-
3197 or contact your local dealer. In Europe, call Ultra-Violet Products Limited in England at +44 (0) 1223-
420022.

2UV Benchtop Transilluminator

The 2UV Benchtop Transilluminator operates the same as the single UV version (Section 5.1) except that
it is equipped with a 365/302nm switch. The transilluminator can be turned on with the UV selector switch
in any position.

3UV Benchtop Transilluminator

The 3UV Benchtop Transilluminator operates the same as the single UV version (Section 5.1) except that
it is equipped with a 365/302/254nm switch. The transilluminator can be turned on with the UV selector
switch in any position. However, when switching between wavelengths, the following should be done to
avoid any merging of wavelengths.

1. Turn main power switch (green) off.

2. Actuate three-position switch to desired wavelength location.

3. Turn main power switch on.

Maintenance/Repair/Technical Assistance

UVP offers technical support for all of its products. If you have any questions about the product’s use,
operation or repair, please call or FAX UVP Customer Service at the following numbers. In the US: toll free
(800) 452-6788; Tel: (909) 946-3197; Fax: (909) 946-3597. In Europe/UK: +44(0)1223-420022 or Fax: +44(0)1223-
420561

Note: A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service
before returning any product.
Benchtop UV Transilluminators Page 5

Single UV Transilluminator Schematics

2UV Transilluminator Schematics

Benchtop UV Transilluminators Page 6

3UV Transilluminator Schematics

Benchtop UV Transilluminators Page 7

Replacing the UV Tubes

In the Single UV Benchtop Transilluminator

Disconnect the transilluminator from the electrical supply.

A phillips head screwdriver is required to remove the filter cover.

Carefully twist the UV tubes from their sockets.

Fit with the proper replacement tubes. Refer to Section 10 for ordering information.

In the 2UV Benchtop Transilluminator

Care must be taken to replace the proper UV wavelength tube into the proper socket location. The 2UV
transilluminator is fitted with alternating 302/365nm tubes: six of the 302nm UV wavelength and five of the
365nm UV wavelength.

Disconnect the transilluminator from the electrical supply. A phillips head screwdriver is required to remove
the filter cover. It is best to remove the tubes in sequence and replace in the same manner. UV tubes 1-3-
5-7-9-11 are 302nm and UV tubes 2-4-6-8-10 are 365nm. Refer to Section 6.2 for a position drawing. Fit
each location with the proper replacement tubes. Refer to Section 10 for ordering information.

In the 3UV Benchtop Transilluminator

Care must be taken to replace the proper UV wavelength tube into the proper socket location. The tubes
in the top row of the 3UV transilluminator consist of alternating 302/254nm tubes. Beneath each 254nm
UV tube is a 365nm UV tube.

Disconnect the transilluminator from the electrical supply.

A phillips head screwdriver is required to remove the filter cover.

It is best to remove the tubes in sequence and replace in the same manner. UV tubes 1-4-7-10-13-16 are
302nm and UV tubes 2-5-8-11-14 are 254nm. To change the 365nm UV tubes (numbered 3-6-9-12-15) in
the second row, it is necessary to remove the top row of tubes. Refer to Section 6.3 for a position drawing.
Fit each location with the proper replacement tubes. Refer to Section 10 for ordering information.

Cleaning the UV Transilluminator

The painted surfaces and filter areas of the transilluminator should be cleaned with water, soap, and a
sponge or cloth towel. Never use abrasive cleaners, solvent based cleaners or scouring pads.

ALWAYS DISCONNECT THE TRANSILLUMINATOR FROM THE ELECTRICAL POWER PRIOR TO

CLEANING.
Benchtop UV Transilluminators Page 8

Replacement Parts and Accessories

For replacement parts or components not shown here, please call UVP Customer Service or place of
purchase. Please have the model number available when you call.

Replacement Part Description Part Number

Filter Assembly (M-10E) 38-0150-01

Filter Assembly (M-15, M-15E) 38-0161-03
Filter Assembly (M-20, M-20E, LM-20E) 38-0161-01
Filter Assembly (M-26, M-26E, LM-26E) 38-0161-02
Filter Assembly (LMS-20E) 38-0163-01
Filter Assembly (LMS-26E) 38-0163-02

UV Tube, 6 Watt, 302nm midrange 34-0044-01

UV Tube, 8 Watt, 302nm midrange 34-0042-01
UV Tube, 8 Watt, 365nm longwave 34-0006-01
UV Tube, 8 Watt, 254nm shortwave 34-0007-01

Ballast , all 115V/60Hz models 42-0005-01

Ballast , all 230V/50Hz models 42-0005-04
Ballast , all 100V/50-60Hz models 42-0005-03
Starter, all 115V/60Hz models 53-0001-01
Starter, all 230V/50Hz models 53-0131-01
Starter, all 100V/50-60Hz models 53-0001-02
Fuse, 1 amp, 250V, Slo-Blo (all voltages) 56-0022-02

Accessories Description Part Number

Gel-Cutter 85-0002-01
Gel-Scooper 85-0006-01
Gel-Tray, UV Transmitting, Small 85-0007-01
Gel-Ruler, UV Fluorescing 85-0003-01
Spectacles, UV Blocking 98-0002-01
Goggles, UV Blocking 98-0002-02
Faceshield, UV Blocking 98-0002-04
Warranty

UVP, LLC warrants its Ultraviolet Transilluminators to be free of detects in materials and workmanship for a period of two
(2) years from date of purchase. The foregoing warranty of UVP shall be of no force and effect if buyer has modified or
damaged the product. Tubes and filters are warranted for 90 days.

All warranties or merchantability and fitness for any purpose and all other warranties, expressed or implied, except
those expressly set forth herein, are deemed waived and excluded.

UVP’s duty under the warranty is limited to replacement and/or repair of the defective part at the option of UVP, Inc.
UVP shall not be liable for any expenses or damages incurred by the purchaser except as expressly set forth
herein, and in no event shall UVP be liable for any special, incidental or consequential damages of any kind. This
warranty does not supersede any statutory rights that may be available in certain countries.

UVP … Providing Quality Products for the Researcher Since 1932

From its start in 1932, UVP has become a story of growth and value fulfillment. Today, UVP services the needs of
science, industry and education throughout the world. A special significance of UVP research and development
arises from our constant communication with customers. UVP continually invites your comments toward the im-
provement of UVP products or the development of custom types of ultraviolet light sources. UVP will work with you
through development and production of a product that meets your specific needs. From all of us at UVP, Thank You.