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    CRP Worksheet

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    Septheia Maeith Vergara
    A student conducted a test for C-reactive protein (CRP) using a latex agglutination method. No agglutination was observed for a patient's serum with an elevated CPK-MB level and symptoms of a possible myocardial infarction. To determine the patient's CRP level, their serum would need to be diluted in a serial two-fold dilution up to the 6th tube, where agglutination was observed, indicating a CRP level of 3.6 mg/dL. Measuring CRP levels can help physicians rule out conditions like myocardial infarction and identify potential causes of elevated CRP such as bacterial infections, tumors, or inflammation.

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    CRP Worksheet

    Uploaded by

    Septheia Maeith Vergara
    15 views2 pages
    A student conducted a test for C-reactive protein (CRP) using a latex agglutination method. No agglutination was observed for a patient's serum with an elevated CPK-MB level and symptoms of a possible myocardial infarction. To determine the patient's CRP level, their serum would need to be diluted in a serial two-fold dilution up to the 6th tube, where agglutination was observed, indicating a CRP level of 3.6 mg/dL. Measuring CRP levels can help physicians rule out conditions like myocardial infarction and identify potential causes of elevated CRP such as bacterial infections, tumors, or inflammation.

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    A student conducted a test for C-reactive protein (CRP) using a latex agglutination method. No agglutination was observed for a patient's serum with an elevated CPK-MB level and symptoms of a possible myocardial infarction. To determine the patient's CRP level, their serum would need to be diluted in a serial two-fold dilution up to the 6th tube, where agglutination was observed, indicating a CRP level of 3.6 mg/dL. Measuring CRP levels can help physicians rule out conditions like myocardial infarction and identify potential causes of elevated CRP such as bacterial infections, tumors, or inflammation.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    15 views2 pages

    CRP Worksheet

    Uploaded by

    Septheia Maeith Vergara
    A student conducted a test for C-reactive protein (CRP) using a latex agglutination method. No agglutination was observed for a patient's serum with an elevated CPK-MB level and symptoms of a possible myocardial infarction. To determine the patient's CRP level, their serum would need to be diluted in a serial two-fold dilution up to the 6th tube, where agglutination was observed, indicating a CRP level of 3.6 mg/dL. Measuring CRP levels can help physicians rule out conditions like myocardial infarction and identify potential causes of elevated CRP such as bacterial infections, tumors, or inflammation.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Institute of Clinical Laboratory Sciences

    SILLIMAN UNIVERSITY
    MT 38 – Immunology & Serology

    NAME: SEPTHEIA MAEITH R. VERGARA & JEANETTE DIANNE S. CATIAN SECTION: F

    Activity 1
    Test for C-Reactive Protein

    Test Principle:
    CRP slide test is based on the principle of agglutination. Serum is mixed with CRP latex reagent and allowed to react. If CRP
    is greater than 0.6 mg/dl, a visible agglutination is observed. If CRP concentration is less than 0.6 mg/dl, no agglutination is
    observed.

    Analysis:
    Why is agglutination not observed when the CRP of the patient is lesser than 0.6mg/dL?

    Agglutination is not observed because the reagent can only detect greater than 0.6 mg/dL CRP concentration, in
    the zone of equivalence, hence, if the sample has less than 0.6mg/dl CRP then no agglutination will occur, this is a
    negative test result which indicates the absence of CRP in the test specimen.

    Computations:
    A patient’s CRP level was determined for a patient to rule out myocardial infarction. The patient has an elevated CPK-MB
    result & positive clinical symptoms. However, the CRP result of the undiluted serum showed a negative reaction. If 0.5 ml
    of serum is to be diluted at 1:2 for semi-quantitative CRP test, how much diluent must you add to make a 1:2 dilution?

    Why is it necessary to dilute the serum until a positive agglutination reaction is observed?
    It is important to dilute the serum until a positive agglutination is observed in order to eliminate cases of Postzone reactions
    in order to achieve a zone of equivalence.

    Why will a prozone or postzone reaction inhibit agglutination reaction?


    Prozone has excess antibody, and postzone has excess antigen which can result in no reaction, not until the
    antibody in the serum has been diluted enough to be in equilibrium with the antigen.

    If serum was diluted in a serial two-fold dilution, what is the final dilution if a CRP positive reaction was observed in the 6th
    tube?

    MT 38 – Serology Worksheets/Silliman University 2015


    What is the CRP level of this patient in mg/dL? (show the computation)
    CRP (mg/dl) = 0.6 X D, where D = highest dilution of serum showing agglutination
    = (0.6) (6)
    CRP (mg/dl) = 3.6 mg/dL

    Dilution: (Diagram)
    Illustrate in this section the serial two-fold dilution procedure used up to the 6th tube. Indicate the dilution factor and
    dilution of each tube. Prepare 6 sets of tubes. To tube # 1, add 0.5 ml serum and the 0.5 ml NSS.
    0.5+0.5 = 1 ml

    DF = Tv/Sv 5th tube


    = 1ml/0.5 DF = (1:16) (1:2)
    DF = 2 or 1:2 = (1:32)
    2nd tube 6th tube
    DF = (1:2) (1:2) DF = (1:32) (1:2)
    = 1:4 = (1:64)
    3rd tube
    DF = (1:4) (1:2)
    = (1:8)
    4th tube
    DF = (1:8) (1:2)
    = (1:16)

    Significance of the Test


    A positive CRP test is indicative of what medical condition? In what way can a determination of CRP levels in a patient assist
    a physician in resolving the health condition of the patient?

    Positive CRP test, is acutely elevated plasma proteins in response to illness, infection, trauma and tissue necrosis. Can be due
    to:
    ● almost always present in: rheumatic fever, rheumatoid arthritis, acute bacterial infections & viral hepatitis
    ● frequently present in: active tuberculosis, gout, advanced malignant tumors, leprosy, active cirrhosis, widespread
    burns & peritonitis
    ● sometimes present in: multiple sclerosis, Guillain-Barre syndrome, scarlet fever, varicella, postsurgical state, use of
    intrauterine contraceptive device
    Hence, the physician can rule out possibilities of Myocardial infarction, through determination of CRP levels. Since the
    patient has a positive result for CRP then it is possible that the patient is not suffering from Myocardial infarction and/or the
    infarct may be caused by the following health conditions mentioned above.

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