Produce Organic Concoctions and Extracts
Produce Organic Concoctions and Extracts
Produce Organic Concoctions and Extracts
RPTESDC-BINANGONAN
1. Fermented Plant Juice (FPJ) – is made from axillary buds and young fruits, fast growing
plants, young leaves of plants and grasses. Mixed with crude sugar or molasses, the juice is
extracted and fermented after storing for a period of time. The liquid is applied to plant’s
leaves and growing points, soil around the plant, compost heap and animal beddings to
fortify microbial activities.
2. Fermented Fruit Juice (FFJ) - is made from sweet ripe fruits, fruit vegetables and root
crops. Thoroughly blended with crude sugar or molasses and stored for a short period of
time, the fermented extract is applied to the plants to promote flowering and fruit setting.
3. Kuhol / Fish Amino Acid (KAA/FAA) - is made from spoiled fish and fish trash such as
bones, head, internal organs and skin with crude sugar; juice of the fish is extracted and
gets fermented after storing for 30-day period.
Is a liquid made from fish waste. FAA is of great value to plants and microorganisms in their
growth because it contains and abundance of nutrients and various amino acids. Blue
Back fishes have good proteins, amino acids, and fatty acids that are recognized as
4. Oriental Herbal Nutrient (OHN)- is used in natural, also known as organic, farming to
restrain the growth of anaerobic bacteria, which usually thrives without the presence of
oxygen, that can cause diseases in plants. OHN is used not only to prevent these
pathogenic bacteria from harming crops, but also to promote aerobic microorganisms that
benefit the soil and plants by improving their resilience to various environmental stresses
like wind, heat, drought, and so on.
OHN is a mixture of edible, aromatic herb extracts. Herbs with prebiotic components such
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 1 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
as ginger root (Zingiber officinale), turmeric root (Curcuma longa), garlic cloves (Allium
sativum), licorice root (Glycurrhiza uralensis), and cinnamon bark (Cinnamomum
zeylanicum) are usually used. The prebiotic elements found in these herbs encourage the
growth and activity of beneficial microorganisms like bacteria and fungi that can help enrich
the soil
7. Lactic Acid Bacteria Serum (LABS)- is now used for its ability to convert waste into
organic matter and basic materials. And they thrive and feed on the ammonia released in
the decomposition normally associated with the foul odor (removes foul odor)
2. wooden ladle
3. weighing scale
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 3 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
4. chopping board
5. knife
7. nylon screen
9. stone
10. marker
11. plastic tray
12. container 60 li
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 4 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
TOPIC 3 STERILIZATION
Staff at production and research laboratories use high-pressure steam inside an
autoclave to sterilize or remove all microorganisms from plastic containers. These
containers must be rated safe for an autoclave as some plastics, such as HDPE and
polyethylene, will melt in the course of a standard autoclave run. For those looking to
sterilize plastic containers at home, a standard microwave oven will do the trick. Of
course, only microwave-safe plastics ought to be sterilized in this manner. Although not
appropriate for home sterilization, plastic container sterilization can also be
accomplished via ethylene oxide 'gas' sterilization, peracetic acid, ionizing radiation, dry
heat, hydrogen peroxide gas plasma systems, ozone, formaldehyde steam, gaseous
chlorine dioxide and infrared radiation.
Microwave Sterilization
1. Prepare a Heat Sink
Fill a cup with 250 to 500 ml (about 1 to 2 cups) of water and place it in the microwave.
This will act as a heat sink to ensure the plastic container inside the microwave doesn't
get too hot and melt.
2. Place Containers in Microwave
Gather together the microwave-safe containers and lids that require sterilization.
Microwave containers in a secondary container for at least 3 minutes on the highest
setting.
3. Take Out Sterilized Containers
Remove secondary container for microwave with plastic containers inside, while
maintaining sterility. Use insulated gloves, as the containers may be hot.
Autoclave Sterilization
1. Prepare the Containers
Gather together autoclave-safe plastic containers and any lids that need sterilization. Lids
can be loosely placed on top of containers. A tightly attached lid can cause a container
to succumb to pressure within the autoclave and crack or explode.
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 5 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
Place containers and lids in a secondary autoclave-safe container, making sure to leave
space between containers.
Place the secondary container in the autoclave and follow any standard operating
procedures for your specific autoclave. The standard sterilizing autoclave run is at 121
degrees Celsius, 15 pounds per square inch of pressure for at least 30 minutes.
Remove the secondary container from the autoclave using thick, insulated gloves to avoid
burning. The surfaces will be extremely hot.
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 6 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 7 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
1. Fermented Plant Juice (FPJ) - At least three kinds of plants but not limited to kangkong,
camote tops, alugbati, malunggay, banana trunks, bamboo shoots and other fast growing
green plants and Molasses/mascuvado/ brown sugar.
2. Fermented Fruit Juice (FFJ) - Ripe and sweet fruits but not limited to banana, papaya,
watermelon, ampalaya , tomato and Molasses/mascuvado/ brown sugar.
3. Kuhol / Fish Amino Acid (KAA/FAA) - Trash Fish and gills, scales, offal of big fishes,
golden kuhol meat and Molasses/mascuvado/ brown sugar
4. Oriental Herbal Nutrient (OHN)- garlic, ginger, pure coconut vinegar and muscuvado
5. Natural Calcium Phosphate Micro Nutrients (CALPHOS)- animal bones, egg shell, sea
shell, kuhol shell and Natural vinegar
7. Lactic Acid Bacteria Serum (LABS)- 900 ml. fresh milk,100 ml clear liquid from fermented
rice and1 liter molasses/brown sugar/mascuvado
when French chemist Louis Pasteur demonstrated that fermentation was caused by yeast.
Fermentation occurs in certain types of bacteria and fungi that require an oxygen-free
environment to live (known as obligate anaerobes), in facultative anaerobes such as yeast,
and also in muscle cells when oxygen is in short supply (as in strenuous exercise). The
processes of fermentation are valuable to the food and beverage industries, with the
conversion of sugars into ethanol used to produce alcoholic beverages, the release of
CO2 by yeast used in the leavening of bread, and with the production of organic acids to
preserve and flavor vegetables and dairy products.
Function of Fermentation
The main function of fermentation is to convert NADH back into the coenzyme NAD+ so that
it can be used again for glycolysis. During fermentation, an organic electron acceptor (such
as pyruvate or acetaldehyde) reacts with NADH to form NAD+, generating products such as
carbon dioxide and ethanol (ethanol fermentation) or lactate (lactic acid fermentation) in the
process.
Types of Fermentation
There are many types of fermentation that are distinguished by the end products formed
from pyruvate or its derivatives. The two fermentations most commonly used by humans to
produce commercial foods are ethanol fermentation (used in beer and bread) and lactic acid
fermentation (used to flavor and preserve dairy and vegetables).
Ethanol Fermentation
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 9 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
Materials: 1 kg banana fruit; 1kg papaya; 1kg watermelon; and 3 kgs molasses
Steps on how to ferment:
1. Clean and wash fruits
2. Drain for 5 minutes
3. Slice to an inch size
4. Mix all fruits thoroughly in a plastic pail ( 20 liters capacity)
5. Mix with 3 kgs. Of molasses thoroughly
6. Put nylon screen on top of the mixture
7. Put 5-8 pieces 25-30 grams stone on top of the nylon screen
8. Wipe the mouth of the plastic pail
9. Cover with two layered manila paper
10. Tie with rubber band
11. Put marking on the masking tape bearing the name and date of fermentation and
paste it on top of the manila paper
12. Keep in dark cool room for 7 days
13. Open the mixture and extract the liquid
14. Filter the liquid and keep it in a plastic container ( do not close the cap tightly, loosen
the cap of approximately 1 complete twist)
15. Completely close cap after a week or when there are no bubbles going up
16. The concoction is ready to use after extraction
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 11 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 12 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
close the cap tightly; loosen the cap of approximately 1 complete twist)
15. Completely close cap after a week or when there are no bubbles going up
16. The concoction is ready to use after extraction.
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 13 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
13. Wipe the mouth of the plastic pail, cover with two layered manila paper and write the
markings (name and date of fermentation)
14. Open the container after 30 days of soaking, filter the liquid and keep it in another
plastic container( do not close the cap tightly, loosen the cap of approximately 1
complete twist)
15. Completely close cap after a week or when there are no bubbles going up. Write the
proper markings. It is ready to use.
Materials: 5 kgs. Celery; 5 kgs. Carrots; 5 kgs. Cucumber; and 5 Kgs. Muscuvado
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 14 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
Supplies/Materials Raw materials for the concoction and extracts, tools and
kitchen equipment
1. Go to the workshop area. Inform your trainer that
you are ready for this activity.
Procedure:
2. Clean and sanitize your hand before you start the
activity.
3. Ensure that all the conditions within the workstation
conforms with Safety and Health
4. Prepare the raw materials and equipment required
to prepare various concoction and extracts.
5. Produce various concoctions and extracts according
to steps on how to ferment.
6. Clean Work area.
Assessment Method Face-to-face feedback from trainer
• Packaging is also used for convenience and information transmission. Packages and
labels communicate how to use, transport, recycle or dispose of the package or product.
Activity Sheet 3
labels
Procedure 1:
1. Prepare all the supplies and materials in labelling and tagging
2. Get your produce concoctions
3. Label the fermented products base on the required information
4. Submit your work to your trainer. After checking, your trainer will return your work.
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 17 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
Keep the refined LAB serum at cool temperature, so for longer period where there is
temperature change (1-15°C ). No storage under direct sunlight.
In order to keep LAB at a normal temperature it must be mixed with the same amount
of brown sugar and stirred with a wooden stick (ladle).
Note: Using rice-washed water in obtaining lactic acid bacteria is to collect stronger ones.
Only strong ones can survive in poor nutrients condition like rice washed water.
Indigenous Microorganism
Keep the IMO3 bags in shaded and cool place. Make sure that the air is well circulated by
keeping IMO-3 in a ventilated container such as jute / gunny / cloth bags.
First, spread rice straw or leaf litter at the bottom of the container, and put in IMO-3. During
storage, the IMO-3 may become dry (moisture level 20-30%) as the moisture gets
evaporated. It means that the IMOs have entered a sleeping phase (state of dormancy). Pile
up containers into 3 layers and shield them from direct sunlight and rain. At this point, there
is no need to turn over, because of the convection currents that are created through the
gaps of containers.
Temperature Control
There should be active mechanical temperature control and no direct sources of heat (sunny
windows, steam pipes, furnaces, etc.).
Most are fairly stable at room temperature. 4 degrees Celsius should be ok for
most plant extracts. I would also suggest keeping it out of light as well. As light can
alter certain compound and decrease their potency and change their structure
Shelf life
• The sealed bottles or other package prevents contamination during storage and
transport.
• The concoctions/extracts will have a long but not an indefinite storage life.
• The storage life depends on several factors, but especially on the nature of the
product and the conditions of storage.
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 18 of 19
Extracts
TECHNICAL EDUCATION AND SKILLS DEVELOPMENT AUTHORITY
RPTESDC-BINANGONAN
https://www.youtube.com/watch?v=GouWt_DM544
https://www.youtube.com/watch?v=Qr3gZAbnrHM
https://www.youtube.com/watch?v=W58mZaJCArA
https://www.youtube.com/watch?v=peJeyMV2Plc
https://www.youtube.com/watch?v=lspuVsEW3vY
https://www.youtube.com/watch?v=6a1YezkoLjs
https://www.youtube.com/watch?v=A40KLz6fRk8
https://www.youtube.com/watch?v=UXysXSFuME0
https://www.youtube.com/watch?v=vCFkmuUL-S0
https://www.youtube.com/watch?v=XzdeuMz3MZ4
Date Developed:
May 5, 2020 Version No. 1
E-Learning for ORGANIC
AGRICULTURE PRODUCTION NC II
Developed by:
Module Title: CONCEPCION T.
Producing Organic Concoctions and OBCENA Page 19 of 19
Extracts