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    Carbocisteine

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    Laura Rosales
    This document describes a new method for determining S-carboxymethylcysteine in cough syrup formulations using high-performance liquid chromatography (HPLC). The current gas chromatography (GC) method is time-consuming, requiring 2-3 hours for sample preparation and derivatization. In contrast, the new HPLC method does not require derivatization and can analyze samples in just minutes, allowing for more samples to be tested in less time. The method extracts S-carboxymethylcysteine from cough syrup using sodium hydroxide, separates it on an aminopropyl silica column using a phosphate buffer-acetonitrile mobile phase, and detects it with a retention time between 5-10 minutes. It was

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    © All Rights Reserved
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    Carbocisteine

    Uploaded by

    Laura Rosales
    74 views1 page
    This document describes a new method for determining S-carboxymethylcysteine in cough syrup formulations using high-performance liquid chromatography (HPLC). The current gas chromatography (GC) method is time-consuming, requiring 2-3 hours for sample preparation and derivatization. In contrast, the new HPLC method does not require derivatization and can analyze samples in just minutes, allowing for more samples to be tested in less time. The method extracts S-carboxymethylcysteine from cough syrup using sodium hydroxide, separates it on an aminopropyl silica column using a phosphate buffer-acetonitrile mobile phase, and detects it with a retention time between 5-10 minutes. It was

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    carbocisteine

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    This document describes a new method for determining S-carboxymethylcysteine in cough syrup formulations using high-performance liquid chromatography (HPLC). The current gas chromatography (GC) method is time-consuming, requiring 2-3 hours for sample preparation and derivatization. In contrast, the new HPLC method does not require derivatization and can analyze samples in just minutes, allowing for more samples to be tested in less time. The method extracts S-carboxymethylcysteine from cough syrup using sodium hydroxide, separates it on an aminopropyl silica column using a phosphate buffer-acetonitrile mobile phase, and detects it with a retention time between 5-10 minutes. It was

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    74 views1 page

    Carbocisteine

    Uploaded by

    Laura Rosales
    This document describes a new method for determining S-carboxymethylcysteine in cough syrup formulations using high-performance liquid chromatography (HPLC). The current gas chromatography (GC) method is time-consuming, requiring 2-3 hours for sample preparation and derivatization. In contrast, the new HPLC method does not require derivatization and can analyze samples in just minutes, allowing for more samples to be tested in less time. The method extracts S-carboxymethylcysteine from cough syrup using sodium hydroxide, separates it on an aminopropyl silica column using a phosphate buffer-acetonitrile mobile phase, and detects it with a retention time between 5-10 minutes. It was

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    of 1

    Journal of Chromatography, 391 (1987) 321-324

    Elsevier Science Publishers B.V., Amsterdam - Printed in The Netherlands

    CHROM. 19 373

    Note

    Determination of S-carboxymethylcysteine in syrup formulations by


    high-performance liquid chromatography

    CHARLES K. MELUCCI*, GARY W. LYMAN, ARTHUR D. BOND and RAYMOND N.


    JOHNSON
    Analytical Research and Services, Ayerst Laboratories. Inc., 64 Maple Street, Rouses Point, NY 12979
    (U.S.A.)
    (First received October 6th, 1986; revised manuscript received December 16th, 1986)

    A simple, rapid, and stability indicating method for the determination of 9


    carboxymethylcysteine in cough syrup has been developed using high-performance
    liquid chromatography (HPLC). In contrast to many other published amino acid
    methods, derivatization of the compound is not required.
    S-Carboxymethylcysteine is an amino acid used in cough syrups as a mucolytic
    to aid in cough suppression. Due to a reformulation study, a great increase of cough
    syrup samples were expected to be tested. As a result of this increase, a simple and
    rapid assay specific for S-carboxymethylcysteine was sought. The current gas chro-
    matographic (GC) assay involves time consuming sample preparation since it re-
    quires derivatizing with N,O-Bis(trimethyl)-trifluoroacetamide-1% trimethylchloro-
    silane (BSTFA-1% TMCS). The estimated sample preparation time using the GC
    assay was determined to be 2-3 h while the HPLC procedure is accomplished in
    minutes. As a result of the shortened sample preparation time, a greater number of
    samples can be assayed in much less time. Other methods for amino acids involving
    derivatization procedures such as dansyll, phenylisothiocyanate2, o-phthaldialde-
    hyde3, and phenylthiohydantoin4*5 were considered more complicated and time con-
    suming.

    EXPERIMENTAL

    SCarboxymethylcysteine is extracted with 0.1 M sodium hydroxide, diluted


    with water and chromatographed on a 15 cm x 4 mm I.D. column packed with
    aminopropyl silica (NH2, 5-pm particles), using phosphate buffer-acetonitrile as the
    mobile phase.
    A peak retention time between 5 and 10 min allows adequate separation of
    degradation products and other components in cough syrup. The method is also
    shown to have the required accuracy, precision, and linearity necessary for stability
    studies and routine assays.

    Instrumentation
    The HPLC system used for this study was composed of the following: A single
    pump isocratic system (Model 590, Waters Assoc., Milford, MA, U.S.A.), an autoin-

    0021-9673/87/%03.50 0 1987 Elsevier Science Publishers B.V.

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