Enzymes Form 3
Enzymes Form 3
Enzymes Form 3
Enzymes are proteins made up of long chains of amino acids. They control chemical reactions that take
place in all living things such as digestion, respiration and photosynthesis. They are known as biological
catalysts as they speed up chemical reactions allowing them to occur at a faster rate. During this process,
however the enzymes themselves do not get used up and so can continue catalyzing the reaction.
Each enzyme has its own specific 3-dimensional structure and is folded into a unique shape which allows
other molecules to fit into it.
The area where these molecules fit into is known as the enzyme’s active site.
The molecules which fit into the active site are knows as the substrate.
The enzyme and the substrate fit together using a lock and key mechanism.
ACTION OF ENZYMES
Enzymes work by intervening in chemical reactions by locking onto the substrate at their
active site and speeding up the reaction. Enzymes can work in different ways, some enzymes
help to break down large molecules into smaller ones (catabolic reaction), others build up
large molecules from smaller ones (anabolic reaction), whilst there are some that help change
one molecule into another.
MR C GAVI PURE BIOLOGY 2021 FORM 3 ENZYMES
One of the fastest enzymes knows is catalase. Catalase is found in many living cells and breaks
down hydrogen peroxide into water and oxygen. Hydrogen peroxide is often formed as a
product of reactions in cells, and can be poisonous if it builds up it is the job of catalase to
remove the harmful hydrogen peroxide
The substrate (hydrogen peroxide) and the enzyme (catalase) are continuously on the move.
Every so often they collide so that the substrate molecule fits into the enzyme’s active site.
Once in the active site the reaction takes place between the substrate and enzyme, the
substrate is broken down into the two products (water and oxygen). When the required
product has been produced, the enzyme releases itself and moves on. The enzyme is not used
up in the reaction and so is ready to work again! No matter what job an enzyme does it will
work in this similar way.
PROPERTIES OF ENZYMES
Enzymes are sensitive and work best under specific conditions. Enzyme activity is affected by
several things and each type of enzyme has its own specific optimum conditions under which
it works best.
MR C GAVI PURE BIOLOGY 2021 FORM 3 ENZYMES
1. Substrate concentration
Enzymes work best when there is a high enough substrate concentration for the reaction
they catalyse. If too little substrate is available the rate of the reaction is slowed and cannot
increase any further.
Sometimes, if there is too much product accumulating, the reaction can also be slowed
down. Therefore it is important that the product is removed regularly.
2. Temperature
Enzymes are affected a great deal by temperature. If the temperature is too cold the enzymes
move around too slowly to meet the substrate and for a reaction to occur, the enzymes are
inactivated. As the temperature increases though, so does the rate of reaction. This is because
heat energy causes more collisions between the enzyme and the substrate. However, as you
will remember all enzymes are proteins and at too high temperatures the proteins break
down. The active site of the enzyme becomes distorted and so the substrate no longer fits
and hence the reaction does not occur. We say that the enzyme has been denatured.
MR C GAVI PURE BIOLOGY 2021 FORM 3 ENZYMES
3. pH
The pH must be correct for each enzyme to work at its best. Different enzymes work best at
different pH values. The optimum pH for an enzyme depends on its site of action. For
example, enzymes in the stomach have an optimum pH of about 2 because the stomach is
acidic, but intestinal enzymes have an optimum pH of about 7.5. If conditions are too alkaline
or acidic for that particular enzyme, then its activity is affected. This occurs because the
enzyme’s shape, especially the active site, is changed so can no longer bind to the substrate
molecule. We say that the enzyme is denatured.