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    Tests Outcomes

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    Oludamilola Kujore

    Copyright:

    © All Rights Reserved
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    Tests Outcomes

    Uploaded by

    Oludamilola Kujore
    26 views9 pages

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    Tests__outcomes

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    26 views9 pages

    Tests Outcomes

    Uploaded by

    Oludamilola Kujore

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    © All Rights Reserved
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    of 9

    The aim of this document is to collect data on Light Progress products’ efficacy for different application

    of UV-technology. As these are conducted by 3rd party companies, we must remain resolute in
    showcasing the results under strict compliance with all applicable regulatory standards. For further
    information, please contact [email protected] with any questions you may have.
    :
    Test 1: Evaluation of Virucide activity against Sars-Cov-2 of
    Light Progress products
    University of Siena, Department of Molecular and Developmental Medicine

    TESTS PARAMETERS:

    Name of product tested: UV BOX E


    2/40H UV Power = 40 W High Output
    lamp Period of analysis: 10/06/20 –
    13/06/20 Temperature of incubation:
    37°C
    Identification of Viral strain: SARS-CoV-2 (Lot: VMR –SARSCPV2 VERO
    E6_28042020) Incubation period: 3 days
    Irradiation time: 2
    minutes Repetition of
    tests: 3 times
    Experiment method:
    Crystals (UV-C permeable) were positioned in the center on the grid, then inoculated with 100 μL of
    viral
    suspension. The suspension virus used was 107.2 TCID50%/mL (7.2 expressed by Log10).

    TESTS RESULTS:

    After Irradiation, Sars-Cov-2 was inactivated with a Log


    Reduction of 5.7 LOG (>99.999%) which means total
    10
    virucidal
    inactivation.
    Test 2: Evaluation of Disinfectant efficacy Light Progress surface
    irradiation product following FDA (Food and Drug Administration)
    protocol described in “Guidance for Industry about enforcement
    Policy for Sterilizers, Disinfectant Devices, Air Purifier during the
    Coronavirus Disease 2019 (COVID-19 Public Health Emergency”
    University of Siena, Department of Molecular and Developmental Medicine

    TESTS PARAMETERS:

    Name of product tested: UV


    PENTALIGHT Microrganisms
    Tested:
    ― Pseudomonas Aeruginosa ATCC 27853
    ― Escherichia Coli ATCC 8739
    ― Stafilococco Aureus ATCC 43300
    ― Salmonella Typhimurium ATCC 23853
    ― Klebsiella Pneumoniae ATCC
    BAA-1705 Inocolum Carriers: 20 cm2
    stainless Steel carriers Concentrations:
    1.5x107; 1.5x106 CFU/mL Exposure Times:
    4, 7, and 10 minutes
    Distance Surface – Source: 3.5 m
    Repetitions: tests were performed 3 times in triplicate between August and September 2020

    Experiment method:
    2 PCB solution to suspend inoculum colonies were spread on the stainless-steel carriers, one placed
    under UV irradiation and the other out of device reach. At the end of the exposure time both the
    samples were transferred into 90 mm Petri dishes and D/E medium added. Plates were incubated at
    36°C for 48h.

    TESTS RESULTS:

    After Irradiation, all Microrganisms tested were eliminated with value from 4 Log (99.99%) to 7
    10
    Log (99.99999%). The higher effect was of course achieved at 10 minutes exposures (distance was
    10
    fixed at 3.5
    m).
    Test 3: Evaluation of Airborne Microorganisms inactivation transiting
    in-duct on a Light Progress UVGI air purifier according to ISO
    15714:2019
    TECNAL Srl - laboratory accredited by ACCREDIA (nr 0299 L) UNI CEI EN ISO/IEC 17025:2018

    TESTS PARAMETERS:

    Name of product tested: UV


    FAN-XS Period of analysis:
    November 2020

    Microrganisms Tested:
    ― Serratia Marcescens ATCC13880
    ― Bacillus Subtilis ATCC6633
    ― Cladosporium sphaerospermum ATCC11289

    UV-C Device features:


    ― UVC lamp power: 40W
    ― - Power supply: 230 Volt -50/60Hz -40Watt
    ― - Maximum AIR FLOW rate: 125 m3/h
    ― - Flow Speed: 2m/sec
    ― - Passage Section area: 0.0166 m2

    Environmental Conditions:
    Temperature: 25°C+-2°C
    Relative Humidity: 50%
    +-10%

    Experiment Method and Goals:


    Besides certifying an Air Purifier itself, the main goal was to assess the performance of our UVGI
    devices for air disinfection, which are usually mounted in AHUs or duct in heating, ventilating and
    air-conditioning (HVAC)

    Bacterial strains were initially reconstituted in broth culture; then the microorganisms are grown on
    plates; until the dilution obtained is the desired CFU/ml concentration for the inoculum. The
    microorganisms are then inserted in the air purifier by an aerosol generator; using a Anderson
    impactor with the cultivation soil plates provided for the microorganism, performs preliminary flow
    checks of the flow generator.
    Connect the aerosol generator in the inlet hole and the impactor Anderson in the output hole of the
    device and start collecting the microorganisms following the operational protocol as per indications of
    point 7.3 of ISO 15714:2019 protocol.
    The test is performed 3 times both with the UVC light OFF and ON.
    The plates are finally placed to incubate for 24 -48 hrs at 32°C+-1°C. For Cladosporium
    Sphaerospermum: 72/120 hours at 25°C+-1°C.

    TEST RESULTS:

    The percentage of inactivation of aero dispersed microorganisms foreseen ISO 15714:2019 technical
    standard; specifically, the following results have been obtained:
    Serratia Marcescens bacterial inactivation: 100% - calculated UVC dose: D=11.58
    J/m2 Bacillus Subtilis bacterial inactivation: 99.99% - calculated UVC dose:
    D=56.56 J/m2 Cladosporium Sphaerospermum inactivation: 44.1% - calculated
    UVC dose: D=276.53 J/m2.

    Germicidal efficacy has been fully demonstrated.


    Test 3:
    Continued

    UV LAMP UV LAMP
    OFF ON

    UV LAMP UV LAMP
    OFF ON

    UV LAMP UV LAMP
    OFF ON

    D90 inactivation dose required


    (J/cm2)
    Cladosporium
    Sphaerospermum
    Bacillus

    Subtilis Serratia

    Marcescens

    0 2 4 6 8 10 12 14
    Sars-COV
    -2

    LIGHT PROGRESS UV-C sources are capable of irradiating the volume of air treated with a sufficient
    amount of energy to break down up to *99.9% of microorganisms tested by Tecnal, laboratory
    accredited UNI CEI EN ISO / IEC 17025: the laboratory carried out germicidal efficiency test using
    various microorganisms such as: Serratia marcescens, Bacillus subtilis, Cladosporium
    sphaerospermum, as prescribed by the ISO 15714 Standard. These elements are representative of
    gram positive bacteria, gram negatives and fungi. Their reduction percentage it is a guarantee of the
    product's ability to break down a wide range of bacteria, viruses, including coronaviruses and other
    microorganisms that are much less resistant to UV-C wavelegth (see complete chart, page 3).
    Test 4: Evaluation of the effect of Light Progress UV-C air
    Purification devices on the microbial and fungi load present in the
    air
    University of Siena, Department of Molecular and Developmental Medicine

    TESTS PARAMETERS:

    Name of product tested: UV FAN


    M2/95HP Period of analysis: April
    2010

    Microrganisms
    Tested:
    ― Mesophyl Load
    ― Psicrofila Load

    Test Method:
    The experimental protocol provides active sampling of 1 m3 air next to the exit slot of the air purifier with
    both UV lamps ON and OFF. Tests have been conducted in a University classroom where
    administration activity and lessons took place.
    Results are expressed as Unit Forming Colonies for Air Cubic Meter (UFC/m3).

    Experiment Goals:
    Indoor pollution concerns confinated rooms such as work places, schools, hospitals, transportantion,
    etc. where we spend most of our time. International scientific community has been investigating for
    years how public health can be affected by poor Indoor Air Quality environments.
    This study regards the purifying effect of UVGI technology provided by LIGHT PROGRESS in spaces
    where a normal working and social activity is held.
    The goal is to prove that there is a certain benefit in using UV air purifier, especially in crowded and
    closed areas, due to the decreasing of Microbial and Fungi load in air.

    TEST RESULTS:
    LIGHT PROGRESS device succeeded in reducing almost completely the total load of both Mesophyl
    and Psicrofilia microorganisms showing almost none UFC/m3 of aspirated air when lamps were ON.
    Chart below shows results.
    Test 5: Raytracing Model for Morgan Stanley HQ Building Air Duct System
    Based on In-Situ Measurements
    HP Environmental Measure & Validation 3rd Party Consultant for Morgan Stanley

    UV-FCU-90WH-B
    Test 6: UV-Fan Test for Sars-Cov-2

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