Bio Treat - For Effective Removal of BOD, COD & Foul Odor

Bio Treat – For effective removal of BOD, COD & Foul odor.
Bio-Culture Introduction
Bio-culture is a liquid concentrate containing a consortium of beneficial microbes, which acts as soil
conditioner. It acts as an anti-oxidant and develops a conducive environment for the crops to grow. Over
100 countries are using this technology successfully today.
Bio-culture includes both aerobic and anaerobic species of microorganisms, which co-exist in an
environment . Bio-culture mainly consists of Lactic Acid bacteria (Lactobacillus spp.), Azotobactor &
Nitrobactor. These microorganisms secret beneficial substances such as vitamins, organic acids, chelated
minerals, enzymes and antioxidants in a synergistic way, when it comes in contact with organic matter.
Microbes present in Bio-culture suppress the growth of harmful microbes and enhance the growth of
beneficial microbes in all the ecosystems. It controls purification by exploiting the fermentation pathway
of decomposition. These microorganisms are neither imported, exotic, genetically engineered or
modified.
Bio-culture induces Bio Augmentation :
Bio Augmentation is the application of a biological treatment, mainly microbes, to the cleanup hazardous
contaminants in soil and surface or subsurface waters. These microorganisms can be used to transform
them to less harmful forms.
The bacteria feed on the contamination, deriving nutrition for growth and for reproduction. Complex
chemical reactions occur, but the result of this natural process is that contaminants are used up
completely or are converted (or cleaved) into an innocuous product such as water and carbon dioxide.
The microbes will survive and consume their contaminant food source until the unwanted pollutant is
remediated.
Application Areas of Bioculture:
 Effluent & Sewage treatment plants.

 Solid waste management- Composting, Press-mud Composting
 Animal waste from poultry, cattle piggeries etc.
 Collection Systems & lagoons.
 Ponds, rivers & lakes.
 Garbage dumps.
Current Practices in ASP processes:
 Cow Dung – leads to coliform, fecal coliform and the stabilization time with cow dung is
normally 90 days.
 Bio-culture - supplements useful bacteria into the ETP which suppress the growth of the
harmful/ pathogenic bacteria & leads to faster commissioning of the ETP.
Timeline for stabilization:

The normal time of stabilizing the ETP with Bio-culture technology is 45 - 60 days as against the 90 days
with conventional process using Cow Dung. Thus Bio-culture helps in faster commissioning of the ETP
plant and makes it efficient to fight shock loads
Data Requirement before Bio-culture execution:
 Last 1 year complete ETP parameter’s (i.e. COD, BOD, pH, TSS, DO etc) data.
 Quarterly Test Reports.
 Quarterly Audited electrical consumption reports for the ETP.
 Monthly consumption of the chemicals.
 Plant layout chart.
Bio-culture Activation Procedure
Bio-culture is present in 5 Lit. PVC cans in dormant state. Before applying it in ETP/STP, it is required to
activate it according to the following table:
BC Mollasses Water Total

1 1 18 20
1 1 28 30
1 1 48 50
1 1 98 100
em
 The ratio of Bio-culture, Mollasses and water for activation depends upon the quantity and
quality of the wastewater.
 After the molasses has been well dissolved (possibly with the help of some warm water), the full
amount of water is filled into the container and Bio-culture is added.
 Bio-culture must be allowed to brew for a minimum 5-6 days in an airtight fermentation tank. A
fermentation tank allows the activation of microorganisms whereby an optimal amount of
beneficial bacteria is obtained.

Dosing
 Apply Activated Bio-culture at the rate 0.2L-1L for every 1000 L of wastewater by dripping.
 There should be proper aeration. Diffusers are preferred over surface aerator.
 The DO should be maintained above 1.5 to 2 ppm.
 Chemicals dosing should be gradually decreased.
 During non production period DAP and Urea should be added in the ETP/STP.
 It will take 45- 60 days for stabilization of the plant.
 The activated Bio-culture must used within two weeks.
How to achieve Electrical Reduction:
 Bio-culture contains photosynthetic bacteria which releases oxygen during the process of
photosynthesis.
 This helps in reduction of the aeration requirement in the equalization tank.
 Initiate the process of electrical reduction after achieving the effluent parameters well within
the PCB norms with DO level ranging b/w 2-4.
 Initially stop the blowers at the Eq. tank for 15-30 min after every 4 hours of continuous
running. Simultaneous monitoring of the DO level should be done to check any decrease in its
value.
 The stoppage time can be increased gradually to 30-40 min after every 2 hrs of continuous
running of the blowers with continuous monitoring of the DO level.
Note: The reduction in Electrical consumption may vary from plant to plant depending upon the
flow parameters & site conditions.
Precautions / Do’s and don’t
 The drums should be neat and clean.

 Chlorine free water should be used for Bio-culture activation.
 Drum must be airtight. & should not be kept in direct sun light.
 Molasses must be dissolved completely before adding Bio-culture.
 The container should be maintained at 25-400C temperature.
 The activated Bio-culture should not be further multiplied or activated.
 Initially high dose of Bio-culture required then, gradually the dose of Bio-culture is reduced.
 Chemical dosing should be reduced gradually.
 Dosing should be done in Equalization tank and Aeration tank.
Benefits of Bio-Culture
 Reduces the BOD, COD & suppresses the foul odor from the STP & ETP.
 No structural changes are necessary for implementation of bioculture.
 Enhance the overall efficiency of plant.
 Reduce the overall chemical consumption.
 Reduction in aeration requirement, electricity requirement is reduced by 10-30%.
 Helps in faster commissioning of the plant.
 Prevents the proliferation of pathogens.
 Eliminate the foul smell.
 Easily converts the organic waste into organic fertilizer.
 Reduces the overall cost of operation.
Common misconceptions about Bio-culture Technology:
A) The stabilization of the ETP shall be achieved within 10-15 days.
 It normally takes 45- 60 days to stabilize the ETP with Bio-culture Technology.
B) The chemical consumption shall stop right from the start of the Bio-culture execution.
 The chemical consumption reduces gradually over the period of stabilization of the ETP.
C) Bio-culture is a chemical product.
 Bio-culture is completely a biological product containing the strains of bacteria naturally

occurring in nature.

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Bio Treat – For effective removal of BOD, COD & Foul odor.

Bio-culture induces Bio Augmentation :

Application Areas of Bioculture:

 Effluent & Sewage treatment plants.

Current Practices in ASP processes:

Timeline for stabilization:

Data Requirement before Bio-culture execution:

Bio-culture Activation Procedure

BC Mollasses Water Total

How to achieve Electrical Reduction:

Precautions / Do’s and don’t

 The drums should be neat and clean.

Common misconceptions about Bio-culture Technology:

A) The stabilization of the ETP shall be achieved within 10-15 days.

C) Bio-culture is a chemical product.

 Bio-culture is completely a biological product containing the strains of bacteria naturally

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