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    Chem 43 - Worksheet3 - NA

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    Fock Student
    This document contains questions about nucleic acids and gene expression. It asks about: 1) Mechanisms of genome folding in prokaryotes and eukaryotes, including heterochromatin. 2) The results of a Meselson-Stahl experiment on an ancient species, and chances of evolution or extinction. 3) Specifying processes and effects of various mutations on prokaryotic and eukaryotic gene expression. 4) Molecular biology strategies to determine inhibition of DNA synthesis, splicing of an intron, and tRNA binding. 5) Identifying the protein and original amino acid from a mutant DNA strand, and comparing charges and pI of mutant and wild-type peptides.

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    Chem 43 - Worksheet3 - NA

    Uploaded by

    Fock Student
    26 views2 pages
    This document contains questions about nucleic acids and gene expression. It asks about: 1) Mechanisms of genome folding in prokaryotes and eukaryotes, including heterochromatin. 2) The results of a Meselson-Stahl experiment on an ancient species, and chances of evolution or extinction. 3) Specifying processes and effects of various mutations on prokaryotic and eukaryotic gene expression. 4) Molecular biology strategies to determine inhibition of DNA synthesis, splicing of an intron, and tRNA binding. 5) Identifying the protein and original amino acid from a mutant DNA strand, and comparing charges and pI of mutant and wild-type peptides.

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    Chem 43_Worksheet3_NA

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    This document contains questions about nucleic acids and gene expression. It asks about: 1) Mechanisms of genome folding in prokaryotes and eukaryotes, including heterochromatin. 2) The results of a Meselson-Stahl experiment on an ancient species, and chances of evolution or extinction. 3) Specifying processes and effects of various mutations on prokaryotic and eukaryotic gene expression. 4) Molecular biology strategies to determine inhibition of DNA synthesis, splicing of an intron, and tRNA binding. 5) Identifying the protein and original amino acid from a mutant DNA strand, and comparing charges and pI of mutant and wild-type peptides.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    26 views2 pages

    Chem 43 - Worksheet3 - NA

    Uploaded by

    Fock Student
    This document contains questions about nucleic acids and gene expression. It asks about: 1) Mechanisms of genome folding in prokaryotes and eukaryotes, including heterochromatin. 2) The results of a Meselson-Stahl experiment on an ancient species, and chances of evolution or extinction. 3) Specifying processes and effects of various mutations on prokaryotic and eukaryotic gene expression. 4) Molecular biology strategies to determine inhibition of DNA synthesis, splicing of an intron, and tRNA binding. 5) Identifying the protein and original amino acid from a mutant DNA strand, and comparing charges and pI of mutant and wild-type peptides.

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    © All Rights Reserved
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    of 2

    CHEM 43 Lec 1 & Lec 2

    WORKSHEET 3_Nucleic Acids (55 pts)


    1. What mechanisms are involved in polynucleotide folding producing a stable
    molecule that can fit into a small area in the cell? Differentiate prokaryotic and
    eukaryotic genome folding mechanisms and explain the presence of
    heterochromatin structures. (6 pts)

    2. Below is the result of the Messelson & Stahl experiment for an ancient species
    discovered in the thawing permafrost: The Messelson and Stahl experiments
    labelled the parental DNA of the species with N15- DNA. Then the organism was
    transferred to a culture medium containing N14-NH4(SO4) as the only source of
    nitrogen for the newly synthesized DNA.

    N14 N14 N14

    N15 N15 N15 N15

    Fo F1 F2 F3
    What are the chances of the species evolving to new species or become extinct?
    Explain briefly. (3 pts)

    3. For each of the ff., specify: (a) The process affected, and (b) the effect on the
    process and (c) whether the effect is on prokaryotes, eukaryotes or maybe both.
    (3 pts. each). Give a general explanation for your answers.
    a. Insertion of G after the 2nd T in the TATA box
    b. Inhibition of splicing by ribozymes
    c. A mutated TBP protein
    d. The δ factor that does not dissociate from the RNA polymerase
    holoenzyme
    e. Exposure to a substance that mutates the Shine-Dalgarno sequence.
    f. Mutation of the template gene such that an ACC was converted to ATC.
    g. Presence of competitive inhibitors of peptidyl transferase.
    h. Mutation of the 23S RNA preventing the binding of the 40S with 60S
    ribosomes.
    i. Mutation of the regulatory region for a repressor protein in the lac operon.
    j. Inhibition of telomerase activity.

    4. What molecular biology strategy can best be used to determine the following: Explain
    your answers briefly. (6 pts)
    a. Inhibition of DNA synthesis
    b. Inhibition of the splicing of one intron from the parental RNA resulting in a longer
    protein product
    c. Failure of t-RNA to bind at the A site of the small subunit of the ribosome

    5. A mutant DNA strand was transcribed then translated to proteins.


    a. What is the protein product of the mutant DNA strand? (3 pts)

    The sequence of the mutant strand is shown below:

    5’-TGCCATAACTGTTCGTACTGGCAAATTGCC-3’
    3’-ACGGTATTGACAAGCATGACCGTTTAACGG-5’
    b. The mutation altered the sequence of the wild type template DNA such
    that a degenerate codon for a basic amino acid in the wild type was
    converted to a non-degenerate codon resulting in the sequence for the
    mutant strand shown. What was the original amino acid? (3 pts)
    c. Compare the charges and pI of the mutant peptide and the normal (wild-
    type) peptide at physiological pH? (4 pts)

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