Cambridge International AS & A Level

* 6 7 4 5 9 3 3 8 5 3 *

BIOLOGY 9700/52
Paper 5 Planning, Analysis and Evaluation May/June 2020

1 hour 15 minutes

You must answer on the question paper.

No additional materials are needed.

INSTRUCTIONS
● Answer all questions.
● Use a black or dark blue pen. You may use an HB pencil for any diagrams or graphs.
● Write your name, centre number and candidate number in the boxes at the top of the page.
● Write your answer to each question in the space provided.
● Do not use an erasable pen or correction fluid.
● Do not write on any bar codes.
● You may use a calculator.
● You should show all your working and use appropriate units.

INFORMATION
● The total mark for this paper is 30.
● The number of marks for each question or part question is shown in brackets [ ].

This document has 12 pages. Blank pages are indicated.

DC (JC/JG) 185288/3
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1 (a) Woodlice are small invertebrates that live in cool, damp places under wood and stones.

Some students determined the respiration rate of woodlice at different temperatures.

Fig. 1.1 shows a single woodlouse.

length 5–25 mm

Fig. 1.1

The students tested the hypothesis:

For each 10 °C increase in temperature the volume of oxygen absorbed will double.

Fig. 1.2 shows the respirometer that the students used.

1 cm3 syringe

tap

scale

woodlice

gauze

carbon
dioxide
absorbent
capillary tubing containing
coloured liquid

Fig. 1.2
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(i) State the independent variable and the dependent variable in this investigation.

independent variable .........................................................................................................

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dependent variable ............................................................................................................

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[2]

(ii) The students decided to measure oxygen uptake for 5 minutes using a range of
temperatures from 5 °C to 35 °C.

Suggest why the students chose this range of temperatures and suggest a suitable
interval that the students should use.

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(iii) State a suitable control for this investigation.

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(iv) State why the carbon dioxide absorbent solution was placed in the tube.

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(v) Suggest one variable in this investigation that cannot be standardised.

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(b) (i) Describe a method by which the students could set up the respirometer and use it to test
their hypothesis:

For each 10 °C increase in temperature the volume of oxygen absorbed will double.

Your method should be set out in a logical order and be detailed enough to let another
person follow it.

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© UCLES 2020 9700/52/M/J/20

(ii) Each 1 cm length of the capillary tubing containing coloured liquid in Fig. 1.2 has a
volume of 10 mm3.

Describe how the students could use their results to calculate the rate of respiration for
each temperature from their results.

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(iii) Use the axes in Fig. 1.3 to show the expected shape of the curve if the hypothesis is
correct.

For each 10 °C increase in temperature the volume of oxygen absorbed will double.

Include the labels and units for each axis.

Fig. 1.3
[3]

(c) The students used the same apparatus to determine the output of carbon dioxide by the
woodlice at 35 °C. The students used this result and the result for oxygen uptake at 35 °C to
calculate the respiratory quotient (RQ).

The RQ was calculated as 0.95.

State what conclusion can be made from this RQ value.

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[Total: 21]
© UCLES 2020 9700/52/M/J/20 [Turn over