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 CAUTIONS
Avoid Strong Shocks!
Handle the microscope gently, taking
care to avoid strong shocks.
When Carrying the Microscope
When carrying the microscope, support
the bottom of the microscope base, The
instrument weighs about 11 ,5 kg. Do not
hold the overhanBirlQ portion of the base,
[3] Place of Use
Avoid the use of the microscope in a dusty
place, or where it may be subject to
vibrations, or exposed to high
temperatures, moisture, or direct
sunlight,
Power Source Voltage and Fuse
Check the power source voltage and fuse
following the procedure on p, 6,
IS: Light Source
The halogen bulb to be used is 12V~
JOOW. Do not use bulbs other than the
one specified In p, 31 (electrical
specifications) .
If a bulb of more than the suggested
wattage is used. the light adjusting circuit
may be damaged.
[Ql Turning on the Lamp
Take care not to touch the lamp housing
when it is lit, and don't bring inflammable
substances such as gasoline. thinner and
alcohol near it. as some parts of the lamp
housing may become very hot while the
Thank you very much for purchasing lamp is on.
a Nikon Microscope. This microscope Changing the Bulb and Fuse
is a high precision instrument with a Before replacing the bulb or fuse. turn
OFF the main switch and disconnect the
very delicate structure and varied power source plug. Replace the halogen
functions. Please thoroughly read this lamp (12V-1 OOW) , making sure that it is
manual first to use the microscope cool enough. Do not touch the glass part
y.tith your bare hands.
correctly.
[EJ Dirt on the Lens
Do not leave dust, dirt. orfinger marks on
the lens or bulb surfaces.
They will prevent you from clearly
observing the specimen.
[9] Focus Knobs
Never attemptto adjustthetightness of the
nghtand left-hand focus knobs ~turning
one, while holding the other. It may cause
problems. Do not turn the coarse focus
knobs past the limit. Avoid turning it
forcibly. because it may cause problems.
 CARE AND MAINTENANCE
OJ Cleaning the Lenses
To clean the lens surfaces, remove dust
uSing a soft brush or gauze. Only when
removing finger marks or grease, use a
soft cotton cloth, lens tissue, or gauze
lightly mOistened with pure alcohol
(methyl alcohol or ethyl alcohol). For
cleaning thElgbjectives of immersiOiloil
l,!!ie only xylene. Do not use xylene for
cleaning the surface of the entrance lens CONTENTS
of the eyepiece tube or the pnsm surface I.NOMENCLATURE ..... "" ...... 3
of the Ultra-Wide Tube "UW"
Observe sufficient caution In handling
II .ASSEMBLY ........ " ........... " .... 5
alcohol and xylene, (they are In flamma- III .MICROSCOPY .............. " .. 14
ble) ,and the ON-OFF of the power source
switch. N .MANIPULATION OF EACH
Cleaning the Painted Surfaces ELEMENT ................ " .. " ..... 18
Avoid~he use ot<:iny organic solven! (for 1) Use of focusing device ···· .... · .. ·18
example, thinner, ether, alcohol) for
2) Use of refocusing stopper ...... 18
cleaning the painted surfaces and plastic
parts of the instrument. We recommend 3) Optical path change-over in the
you use the attached SIlicon cloth, trinocular eyepiece tube ......... 19
J] Never Attempt to Dismantlel 4) Diopter adjustment .............. 20
Never attempt!o dismantle the
instrument because you may impair the 5) Interpupillary distance
functions. adjustment ........................... 20
@] When Not in Use 6) Centering the condenser lens 21
When not in use, cover the Instrument
with the accessory vinyl cover, and store
7) Use of field diaphragm ......... 22
it in a place free from moisture and 8) Use of condenser aperture
fungus. It is especially recommended diaphragm ........................... 22
that the objectives and eyepieces be kept
In an airtight container containing 9) Use of filters ........................ 22
deSiccant.
10) PHOTO voltage switch ........ · .. ·24
[8 Periodic Checking
11) Oil immersion manipulation .. ·25
To maintain the best performance of the
Instrument, we recommend that the V.TROUBLESHOOTING ,,· .. · .. ·26
Instrument be periodically checked. (For
ELECTRICAL SPECIFICATIONS. 31
details of this check, contact your au-
thorized Nlkon distributor.)
*Please note per your Nikon warranty,
"Any defects or damage directly or indi-
rectly caused by the use of unauthorized
replacement parts and/or performed by
unauthorized personnel" will void the
warranty.
 I • NOMENCLATURE

MICROFLEX clamp screw

Eyepiece tube clamp screw

Optical path

Mechanical
Hexagon wrench
Specimen slide)
Coarse torque
adjustment ring
Specimen holder

Fine-focus scale

Filter holder
clamp screw
Condenser centering
screw

Condenser carner

NO filter insertion
removal knob

Field diaphragm ring

X aXIs motion control knob

Y -axIs stage motion control knob

NO filter cassette

Line indication plate

Line

Fig.1-1

3
 I . NOMENCLATURE

Refocusing stopper Interpupillary

clamp ring distance scale

Objective

Stage clamp screw

Condenser

Condenser aperture
diaphragm ring

Transparent cover

lamp bnghtness indicator

(Indicates also the main
switch and PHOTO voltage)
Bnghtness adjuster

Power sWitch

Condenser clamp screw

Condenser focus knob

Armrest

Power cord

Fig.1-2

4
 II . ASS EMBLY (mounting and dismantling)
To assemble the microscope, follow the procedures given from to@.
For details, read p.6 to p.13.

t@@

Tools: hexagon wrench (accessory). plus screwdriver, minus screwdriver

5
 II .ASSEMBLY

(i) Check the Power Source Voltage and Fuse

• Disconnect the power cord from the AC input connector. ( Make
sure that it is disconnected,)
• Make sure that the line voltage indication plate at the rear of the base
Indicates the correct line voltage to be used (1 00-120V or
220-240V) (Fig. 2 1).
When it indicates incorrect voltage, remove the line voltage
indication plate by unscrewing the two screws using a plus screw-
driver.
• Mount the line voltage indication plate after switching the input
voltage switch to indicate correct voltage.

, ,,-

100V-120V or f~---
220V-240V

Fig. 2-1 Fig. 2-2

• Remove the fuse holder at the bottom of the base using a plus screw
driver (Fig. 2 2) and make sure that the metal part of the fuse
Indicates" 125V 3,1 5A" for 1 00 1 20V or "250V T2A" for
220-240V,
When Incorrect fuse type IS to be Inserted , please contact
your authorized Nlkon distributor .
• Insert the fuse Into the fuse holder and tightly screw it Into the
original position uSing a plus screwdriver,
Leveling Foot Screw
• For stable installation of the microscope, manipulate the
adjustment screw at the rear-left of the base (Fig. 23).

Fig. 2-3

6
 II .ASSEMBL Y

Q) Mounting Lamp and Lamp Housing

Keep thegQver on or use gloves whE:lllJ!lounJl!ill_the Jam!;> bulb so..
as not t() touch ~he surfa~ directly with yoW fingers.
iflinger marks or dirt on the bulb surface, wipe them off with lens
tissue. Make sure to remove the cover after mounting the lamp.
• Release the housing cover clamp screw using a COin [[I to remove
the cover ~ .
• While pulling down the lamp clamp lever ~,firmly insert the lamp
into the socket pin holes until it reaches the limit ~ (Fig. 24).
Caution:
Be careful not to tilt the lamp when mounting. If tilted, try again.
• Return the lamp clamp lever to the original position.
• Close the cover and fasten it with the lamp housing cover clamp
screw .
• Insert the lamp housing into the lamp housing mount of the
microscope base fitting the groove of the lamp housing to the
turning limit pin at the lamp hOUSing mount [Q] and fasten it firmly
with the clamp screw of the lamp housing ~ (Fig. 2 5).

Fig. 2-4 Fig. 2-5

Cautions when dismantling

• Turn off the main sWitch. (Make sure that it IS ofLl Do not touch
the bulb immediately after turning it off because it is very hot. Make
sure that the bulb has cooled enough when replacing it.
• To remove the lamp housing, remove the filter holder. release the
lamp housing clamp screw and pull out the lamp housing from the
base.

7
 II.ASSEMBLY

@ Make Sure of the Position of the Substage

e Make sure that the white e mark on the substage side lines up with
the white <II1II mark on the microscope stand side (Fig.
elf they don't meet. release the substage clamp screw using a minus
screwdriver. Support the substage by hand so it doesn't drop.
Move the substage vertically to adjust the marks and then fasten it.

Fig. 2-6 Fig. 2-7

@ Mounting and Dismantling the Revolving Nosepiece

e Lower the substage by turning the coarse focus knob.
e Loosen the revolving nosepiece clamp screw sufficiently using the
hexagon wrench. (See .)
eTo mount the revolving nosepiece push it In so the groove of the
revolving nosepiece engages with the pin of the circular dovetail [I] .
Fasten it with the revolving nosepiece clamp screw (Fig. 2- 7).
Cautions when disITl(:intling
e Remove the ND filter cassette when it is attached and lower the
stage by tu rn ing the coarse focus knob.
e Remove the objective If attached.
eTo remove the revolving nosepiece, sufficiently loosen the revolving
nosepiece clamp screw using the hexagon wrench. Support the
revolving nosepiece so it doesn't drop.
*Reversed revolving nosepi~c:e and forward revolving nosepiece
Though the standard mounting method of the revolving nosepiece
of this microscope IS backward (reversed), it can also be mounted
facing forward.
Use the following procedures to mount forwardly.
e Loosen the three bolts, with hexagon holes. on the cover of the
nosepiece mount using the hexagon wrench @)to remove
the cover [2].
Keep the three bolts, however, screwed in (Fig. 2-8).

8
 1l.ASSEIVIBLY

Fig. 2-8

• Loosen the three bolts with hexagon holes on the circular dovetail
for mounting the eyepiece tube (Integrated with the Circular dovetail
for mounting the revolving nosepiece) by using the hexagon wrench
[31. Remove the circular dovetail f4'.Mount and turn it 180~ f5 and 12l,
then fasten it with the three bolts with hexagon holes firmly and
equally LZl (Fig. 2-9).
(When removing the circu lar dovetail, keep the revolvlrlg nosepiece
clamp screw fastened.)

Fig. 2-9

• Sufficiently loosen the revolving nosepiece clamp screw using the

hexagon wrench. To mount the revolving nosepiece push It so
the groove of the revolving nosepiece engages with the pin of the
circular dovetail 1Sl. Fasten it with the revolving nosepiece clamp
screw ~ (Fig. 2-10).
When mounting forwardly, the clamp screw is on the right side of
the arm.

9
Fig. 2-10
 II.ASSEMBLY

(6) Mounting and Dismantling the Objectives

e Mount the objectives into the holes of the revolving nosepiece in
such positions that, when viewed from above, their magnifying
power increases as the nosepiece IS revolved clockwise (Fig. 2-11).
Cauti()~r1s when di~f'llantling
e Remove the NO filter cassette if attached and lower the stage by
turning the coarse focus knob.
e Remove any specimen if it is on the stage.
e Hold the objective with both hands so as not to drop it when
removing it from the revolving nosepiece.

Fig.2-11 Fig.2-12

Mounting the Stage

elower the su bstage by turning the coarse focus knob.
eloosen the stage clamp screw sufficiently.
elnsert the stage into the circular dovetail of the stage mount of the
substage, []J and 121. Fasten the stage with the stage clamp screw
~{Fig. 2-12}.
Specimen Holder
e The specimen holder should be fastened to the mounting hole on
the right side for a left-knob stage and on the leftsidefor a nght-knob
. Remove the specimen holder when observing a large
specimen.
(,ID Mounting and Dismantling the Condenser Lens
e Raise the substage by turning the coarse focus knob.
elower the condenser carrier to its limit by turning the condenser
focus knob.
elnsert the condenser lens into the condenser carrier [] with the
aperture number plate facing toward the user. Fasten it with the
condenser clamp screw (Fig. 2-13).
e Raise the condenser carrier to its limit by turning the condenser focus
knob.

10
 II .ASSEMBLY

Cautionswhen Dismantling
e Remove the NO filter cassette if attached.
e Lower the condenser carrier to its limit by turning the condenser
focus knob. Remove the condenser lens by releasing the condenser
clam p screw.

Fig. 2-13 Fig. 2-14

® Mounting the Trinocular Eyepiece Tube

e Loosen the eyepiece tube clamp screw sufficiently using the
hexagon wrench. (See @.)
el nsert the circular dovetail of the trinocular eyepiece tube into the
circular dovetail of the eyepiece tube mount. Fasten It With the
eyepiece tube clamp screw (Fig. 2-14).
@ Mounting the Eyepiece
e Use the same magnification eyepieces for both the right and left
eyes.
elnsert the eyepieces into the sleeves of the tnnocular eyepiece tube
by engaging the three grooves of the eyepiece with the three
protrusions of the sleeve (Fig. 2··1 5).
e When uSing eyeguard rubbers. insert them into the eyepieces (Fig.
2~ 16).

Fig.2-15 Fig.2-16

..
11
__ _---
..
 II .ASSEMBLY

@ Mounting and Dismantling the Filter

When mounting/dismantlingJbl:!Jilter to/from the filter holder, use
gloves or g(3l,1:z:~ so as not to touch the filter directly with your bare
hands.
• Mount the filter to be used as shown in Fig. 2-17 - m. NCB 11 , GI F ,
and NO filters should be mounted to match their respective
indications. The HE filter can be mounted uSing the position for
either the NCB 11 or GIF filter, or to a filter holder with an NO
indication.
• Push the filter holder down into the filter holder mount (Fig. 2 18).
Keep the three filter holders mounted to give protection from dust
even when the filters are not mounted.
• When removing the filter, push the filter as shown in Fig. 2-17-

Fig. 2-17 Fig. 2-18

@ Mounting and Dismantling the ND Filter Cassette

• Push down the NO filter cassette from the top so the two protrusions
of the lower part of the NO filter cassette fit with the two mounting
grooves on the rim of the field lens of the microscope base (Fig.
219) .
• When frequently lowering the microscope stage, mount the NO
filter cassette in the direction as shown in Fig. 2·20 .
• When dismantling, push the cassette either to the left or right and
raise the opposite side.

Fig. 2-19 Fig. 2-20

12
 II .ASSEMBLY

@ Connecting the Power Cord

-Insert the socket of the power cord to the AC input connector and
plug it into a AC line receptacle (Fig. 2-21).
-When the receptacle is not a double-pole ground type, use a plug
converting adapter (Fig 2-22).

Socket

[PJ LIVE,

Ground
terminal

Fig. 2-21 Fig. 2-22

Hexagon Wrench
- The hexagon wrench is stored in the back of the microscope stand.
Take it out as shown in Fig. 2 23.

Fig. 2-23

13
 III. MICROSCOPY
1------s;~~~~Fl--11) Turn the lamp on by setting the main switch to ON.
Set the lamp brightness indicator to "9" using the
brightness adjuster or by turning the PHOTO voltage
switch to ON (Fig. 3-1).

Fig.3-1
2) Slide the filter insertion / removal knobs of the ND
filter cassette to the limit to place the entire ND filter
in the optical path (Fig. 3-2).

Fig.3-2
3) Put the NCB 11 filter (or necessary filter) into the
optical path (Fig. 3-3).

-----::---

Fig.3-3
4) Revolve the revolving nosepiece to put the lOX
objective into the optical path. Properly turn the
revolving nosepiece so it fits into position (Fig. 3-4).

10x

Fig.3-4
5) Place the specimen (glass slide) on the stage with its
Cover cover glass facing up and fasten it with the specimen
glass holder (Fig. 3-5).

Fig.3-5
6) Raise the condenser lens using the condenser focus
knob until it hits the limit (Fig. 3-6). When using an
achromatic aplanat condenser. add oil taking care
not to allow bubbles t6 exist between the top of the
condenser lens and the specimen. (See p. 25.
Manipulation of Each Element-11).)

14 Fig.3-6
 III. MICROSCOPY

7) Fully open the field

diaphragm and the aperture
diaphragm (Fig. 3-7).

8) Manipulate the Yaxis and X-axis stage motion control

knobs to put the specimen into the optical path.
(Place the specimen immediately above the
condenser lens) (Fig. 3-8).

Fig.3-8
9) Loosen the refocusing stopper clamp ring until it
reaches the limit (Fig. 3-9) (See p. 18. Manipulation
of Each Element-2).)

Fig.3-9
~ Observation 10) Change the optical path ofthe eyepiece tube to enter
I \ tube: 100% 100% of the light Into the binocular part (Fig. 3-10).

~­
(See p. 19. Manipulation of Each Element-3}.)

~
1 1) Manipulate the coarse/fine focus knob and focus on
the specimen while looking in the eyepiece
(Fig. 3-11).

12) Adjust the diopter (Fig 3-12). (See p. 20.

Manipulation of Each ElementAl.)

Fig.3-12
15
 III. MICROSCOPY

13) Adjust the interpupillary distance (Fig. 3-13). (See

p. 20, Manipulation of Each Element-5).)

Fig.3-13
14) Focus and center the condenser lens (Fig. 3-14).
(See p 21 , Manipulation of Each Element-6).)

Image of field
diaphragm

Eyepiece
~ vlewfield stop
Fig.3-14
15) Revolve the revolving nosepiece to the objective to
be used and focus on the specimen by manipulating
the fine focus or coarse focus knob. (See l\lote: 2 )
When uSing an oil immersion objective, add oil
taking care not to allow bubbles to exist between
the top of the objective and the specimen. (See
p. 21 , Manipulation of Each Element-11l.)

16) Adjust the brightness by sliding the filter

insertion / removal knob of the NO filter cassette
p. 22. Manipulation of Each Element-g).)
When adjusting the brightness of the lamp,
manipulate the brightness adjuster after turning the
PHOTO voltage setting sWitch to OFF.

17) Adjust the viewfield diaphragm and the aperture

diaphragm by manipulating their control rings.
(See p. 22, Manipulation of Each Element-7) and
8).)

16
 III. MICROSCOPY

Note 1: Manipulate the centering screws of the condenser lens if part of the viewfield
is dark. If this doesn't help, check the following items:

• Insertion / removal of the ND III. MICROSCOPY-2)

filter cassette
• Turning the revolving III. MICROSCOPY-4)
nosepiece(click-stop position)
• Position of the condenser lens Ill. MICROSCOPY-6)
• Viewfleld and aperture diaphragms III. MICROSCOPY· 7)
fully open
• Change-over of the optical path of III. MICROSCOPY -10)
the eyepiece tube
· Mounting the lamp II. ASSEMBLY-@ Mounting the
lamp and the lamp housing
• Mounting the revolving nosepiece II. ASSEMBLY-@ Mounting the
revolving nosepiece
• Mounting the condenser II. ASSEMBLY-@ Mounting the
condenser
• Mounting the filter holder II. ASSEMBLY-([D Mounting the filter

Note 2: Check the following items when the specimen cannot be focused.
• Mounting the specimen III. MICROSCOPY-5)
• Releasing the refocusing stopper III MICROSCOPy-g)
clamp ring
• Position of the substage II. ASSEMBLY -@ Checking the
substage position
• Thickness of the cover
glass(standard =0.1 7mml

17
 N MANIPULATION OF EACH ELEMENT
I

1) Use of Focusing Device

The turning direction of the knob and the direction of vertical
movement of the stage are shown in . 4.
One rotation of the fine focus knob moves the stage O. 1 mm and the
graduation on the fine focus knob is 1 micron. One rotation of the
coarse focus knob moves the stage 12 mm. The range of coarse
and fine motion is 2mm up and 28mm down from the standard
position.
The coarse focus knob tightens by turning the torque adjustment ring
cou nterclockwise.
Never turn the right or left knob while holging the other. It mCl~
cause problems.
Do not turn the coarse focus Imob further than th~ limit.

Fig. 4 Fig. 5

2) Use of Refocusing Stopper

• Turn the refocusing stopper clamp ring and clamp it at the
position where the specimen is focused. It is clamped at the
position where the black delta mark ("') on the Side of the clamp
ring reaches the top (Fig. 5).
• Lower the stage by using only the coarse focus knob and replace
the specimens.
• Raise the stage slowly until it reaches the limit using only the
coarse focus knob.
The specimen should be almost focused at the position where the
stage reaches the limit. Then focus accurately by turning the fine
focus knob.
When the refocusing~topper is not used, loosen the refocusing
stopper clamp ring until it reaches the limit. (See Fig. 3-9.)
When the refocusing stopper clamp ring is clamped, the stage
cannot be raised from that position using the coarse focus knob.
However, the fine focus knob can move the stage regardless of
the limit.

N. MANIPULATION OF EACH ELEMENT

3) Optical Path Change-Over in the Trinocular Eyepiece Tube

(1) When using the trinocular eyepiece tube "F"
As shown in Fig. 6-1 , when the observation tube is turned toward
the user, 100% of the light enters the observation tu be.
As shown in 6-2, when the observation tube IS revolved 60° left
100% of the light enters the vertical photo tube. In either case tur~
the tube to the limit. '--

~ Observation tube:
~ Vertical photo tube: I
 N. MANIPULATION OF EACH ELEMENT

3) Optical Path Change-Over in the Trinocular Eyepiece Tube

(1) When using the trinocular eyepiece tube "F'
As shown in Fig. 6-1 , when the observation tube is turned toward
the user, 100% of the light enters the observation tube.
0

As shown in Fig 6-2, when the observation tube is revolved 60 left,

100% of the light enters the vertical photo tube. In either case, turn
the tube to the limit.

Observation tube: Vertical photo tube:

100% 100%

Fig. 6-1 Fig. 6-2

(2) When using the trinocular eyepiece tube "T"

As shown in Fig. 7, when the change-over knob is pushed until it
reaches the limit [11 100% of the light enters the observation tube.
When the change-over knob reaches the intermediate click [g], the
proportion of light entering the observation tube and photo tube will
be 14:86. When the change-over knob is pulled to the limit ~,
100% of the light enters the photo tube.

IT: Observation tube 100%

~
12l0bservation tube:
photo tube=14:86

_.ccc(r:!t. Photo tube 100%

++

Fig. 7

19
 N. MANIPULATION OF EACH ELEMENT

4) Diopter Adjustment
(Before adjusting the diopter, implement MICROSCOPY procedures
1) to 11) and focus on the specimen with the 10X objective.)
Make diopter adjustments for both the right and left eyepieces.
(1 ) Turn the diopter compensation rings on each eyepiece until the end
su rface of the ring coincides with the engraved line"
(This is the position of a dioptic compensation.) (Fig. 8-1)
(2) Swing the 40X objective by turning the revolving nosepiece and
bring the specimen image into focus by turning the fine focus knob
(or the coarse focus knob)"
(3) Swing the 4X or 1OX objective into position. Without manipulating
th~Jine and COl3rse focus knobs, turn the diopter rings on the
eyepieces so that the specimen images in the right and left
eyepieces are focused individually (Fig 8-2).

End surface

";F:'::=::::::::::~-Engraved line

Fig.8-1 Fig.8-2

• Repeat the above procedure two times, to adjust the diopter

perfectly .
• The above adJustment. compensating diopter difference between
the user's right and left eyes. will keep the tube length of the
microscope correct. This enables the user to take full advantage of
the high-quality objectives, includ ing their parfocality.

5) Interpupillary Distance Adjustment

(Before adjusting the interpupillary distance. implement Microscopy
procedure 1) to 12) and focus on the specimen with the 10X
obJective. )
Adjust the interpupillary distance. so that both the right and left
viewfields become one (Fig. 9).
This adjustment will enable the user to observe with both eyes.

l
20 Fig.9
 N. MANIPULATION OF EACH ELEMENT

6) Centering the Condenser Lens

(Before tocuslng and centering the condenser lens, Implement
Microscopy procedure from 1) to 13} and focus on the specimen with
the 10X objective,}
(1) Close the field diaphragm to its smallest size by manipulating the
field diaphragm control ring. Rotate the condenser focus knob to
move the condenser vertically so that a sharp image of the field
diaphragm is formed on the specimen surface (Fig. 10 1 .2 and

Fig.10-1 Fig.10-2 Fig. 10-3

(2) If the image decenters from the viewfleld of the eyepiece, bring it
roughly to the center of the viewfleld by means of the condenser
centering screws (Fig, 11 -1 and 2).
(3) Change to the 40X objective, Focus on the specimen by turning
the fine focus knob and form an image of the field diaphragm on
the specimen surface by manipulating the condenser focus knob.
(4) When the image decenters from the viewfield of the eyepiece, bring
it to the center of the viewfield by means of the condenser centering
screws, At this time, the centering will be facilitated by adjusting
the size of the image of the field diaphragm to be slightly smaller
than the viewfleld of the eyepiece by manipulating the viewfield
diaphragm control ring . 11 -3).

Fig.11-1 Fig.11-2 Fig. 11-3

21
 N. MANIPULATION OF EACH ELEMENT

7) Use of Field Diaphragm

The field diaphragm is used for determining the illuminated area on
the specimen. The size of the diaphragm is adjusted by manipulating
the field diaphragm control ring. Stopping down the diaphragm to
such an extent that the circumference of the illuminated area
circumscribes or inscribes that of the eyepiece field of view is
recommended for general microscopy. If a wider area than required
is illuminated, extraneous light will enter the field of view, causing flare
in the image and lowering the contrast. Therefore, especially in
photomicrography, the proper adjustment of the field diaphragm is
very important. Generally, good results will be achieved when the
diaphragm is stopped down to such an extent that the diameter of the
illuminated area is slightly larger than the diagonal of the film format.

8) Use of Condenser Aperture Diaphragm

The condenser aperture diaphragm is provided for adjusting the
numerical aperture (N .A.) of the illuminating system of the
microscope. It is important because itdetermines the resolution of the
image, contrast, depth of focus, and brightness.
Stopping down the aperture diaphragm will lower the resolution and
brightness but increase the contrast and depth of focus. In general,
when it is stopped down to 70% to 80% of the numerical aperture
of the objective, a good image of appropriate contrast will be
obtained (Fig. 12).
To adjust the size of the condenser aperture diaphragm, manipulate
the diaphragm control ring referring to the condenser's N.A. scale,
or after removing the eyepiece from the eyepiece tube, adjust the size
of the diaphragm, observing the image of the diaphragm that is
visible on the bright circle of the exit pupil on the inside of the obJective.
Stopping down the aperture diaphragm too far will lower the
resolving power. Therefore, it is recommended not to stop down the
aperture to a size smaller than 60% of the N. A. of the objective in
use, except when observing almost transparent specimens.

Exit pupil of objective

Aperture diaphragm

Size of the condenser aperture diaphragm

Fig. 12
9) Use of Filters
(1) N D filter cassette
Accessory filters attached to the NO filter cassette are N02, N04,

22 and NO 16. These are used for general microscopy and brightness
control in photomicrography.
 N. MANIPULATION OF EACH ELEMENT

ND2 Reduce light to 1/2 I Transmission rate =about 50%

I ND4 Reduce light to 1/4 iSSIon rate about 25%
I ND16 Reduce light to 1 i 16 1 ...
.....L _T~ra~n~s~m~ls~s~lo~n........r.a~t~e~=~a~b~0~ut~6~o/<~o---,
• Light is reduced to 1/8 when the ND2 and ND4 filters are put into
the optical path and is reduced to 1/64 when ND4 and ND16 filters
are put into the optical path Light can be reduced from 1/2 to 1/
128 depending on the combination of the filters .
• Securely change the filter insertion /removal knob.
Removing and mounting the I\lD filters
Use gloves or gauze so as not to touch the filters with your bare
hands.
To remove the ND filter, lay soft cloth such as gauze on a desk.
Place the ND filter cassette on it and spread the lever (Fig. 13).
To mount the filter, insert the filter obliquely in the opposite side of
the lever and spread the lever from the lower side.

Fig. 13

Transparent Cover
Mount the transparent cover to protect the filters from dust (Fig. 14-1)
When the N D filter cassette is removed, attach the transparent cover
on the field lens (Fig. 14-2).
However, remove the cover dunng observation or photomicrogra-
phing with the viewfield diaphragm stopped down.

Fig. 14-1 Fig. 14-2

23
 IV. MANIPULATION OF EACH ELEMENT

(2) Filter holder part

(For the details of attaching/detaching of the filter to/from the filter
holder, see II. ASSEMBLY.)
• Less frequently changed filters are mounted to the filter holder.
These are used when it is too bright, using only the ND filter
cassette.

For general rmcroscopy and color

NCB 11 Colo' balancing fliter
photomicrography
r-------~~ ... --------------------+_-- ------------~
Fa. microscopy of phase
difference, etc.
GIF Green Interfe.ence

I
For hematoxylin'80s r staining
HE I HE color contrast filter
For fuchsine staining

Filters With an outer diameter of 45mm and thickness less than 3mm
can be mounted in both the N D filter cassette and the filter holder.

10) PHOTO Voltage Switch (Photomicrography)

A constant voltage should be used for color photomicrography
because the color temperature of the lamp varies with the voltage bemg
used, The color temperature balancing filter (NCB 11) should be used
when daylight-type color film is used, The standard is to set the voltage
of the lamp to 9V,
The PHOTO voltage switch is provided to set the standard lamp voltage
automatically. The lamp voltage is 9V when it is turned ON. (The
position of the brightness adjuster does not matter,)
Fine adjustment of the PHOTO voltage
The PHOTO voltage of 9V can be finely adjusted by about ~O.5V.
+0, 5V, or+ 1V, When the color film photographed at 9V is reddish.
the voltage should be raised, (A higher lamp temperature gives a bluish
color.) When bluiSh, the voltage should be reduced, (A lower lamp
temperature gives a reddish color,)
To make the adjustment, use the rotary switch at the bottom of the
base with a minus screwdriver (Fig. 1 5),
When this adjustment is inadequate, also use color compensating
filters (CC filters).

Fig. 15

24
 N. MANIPULATION OF EACH ELEMENT

11) Oil Immersion Manipulation

The objectives with the indication "oil" are to be Immersed in oil between the
specimen and the front lens of the objective. Use only the included oil.
When using an oil Immersion objective of numerical aperture 1 .0 or larger, it IS
recommended to use an all Immersion type achromat!aplanat condenser, to make
full use of its efficiency. For this type of condenser, apply oil between the
specimen and the condenser lens.
Apply the minimum amount necessary (the quantity that fills the gap between the
front of the objective and the specimen, or between the front of the condenser lens
and the specimen) to avoid a flow of excessive oil that will adhere to the stage and
the circumference of the condenser lens.
To see it air bubbles are present in the ImmerSion oil, which deteriorate the Image
quality, pullout the eyepiece from the eyepiece tube, Fully open the viewfield
diaphragm and the aperture diaphragm to examine the objective exit pupil (bright
circle) inside the tube.
To remove air bubbles, slightly rotate the nosepiece several times (or turn the
condenser focus knob to move it slightly up and down), or apply additional 011, or
replace the oil.
Any remnant of oil left on the oil immersion objective or adhesion of oil to the front
of the ary system objective will deteriorate the Image quality. Clean off the oil after
using it and make sure that the oil did not adhere to the front of other obJectives,
The oil for the condenser lens should also be cleaned off after use.
To clean off the all, wipe it with lens tissue or a soft cloth, moistened with xylene,
lightly two or three times. It is essential at this time to avoid touching the lens with
a part of tissue or cloth alreaay used.

25
 v TROUBLESHOOTING
•

SEEING AND OPERATION

Failures Causes Actions

Optical path in trinocular tube not fully

changed. Changeover the optical path
~-----~-

securely to enable the

Optical path In trinocular tube not binocular observation
Ichanged-over for binocular ip. 19)
observation
~~-- ............

! Revolving nosepiece not mounted Mount firmly.

Darkness at the i correctly (p.8)
periphery.
Revolving nosepiece not in click-stop I Revolve It to click-stop position
position (objective not centered In (put objective Into optical
Uneven optical path) path). (p.14)
brightness of
viewfield. POSition correctly so the
Condenser IS too low. viewfleld diaphragm Image IS
formed. (p. 21)
No appearance j-----~-

of viewfield. Condenser not centered. 19. (p. 21)

Condenser not mounted mectly correctly (p.10}

Changeover to the limit.

NO filter not fully changed-over
(p. 23)

Mount
Filter holder not mounted correctly.
position

Field diaphragm closed too much. Open properly. (p. 22)

I Improper combination of objective ! Use proper combination.

and condenser.
• Dirt or dust on the lens (field lens,
condenser, objective, eyepiece,
Cleaning. (p. 2)
eyepiece tube entrance lens) or
speCimen.

Lamp not mounted correctly. Mount properly. (p.7)

26
 V. TROUBLESHOOTING

Faures Causes Actions

Position correctly so the

Position of condenser too low. viewfield diaphragm Image IS
formed (p.21)
Dirt or dust in Aperture diaphragm too restricted Open properly. (p 22)
the viewfield.
Dirt or dust on the lens (field
lens. condenser, objective. eyepiece.
Cleaning (p. 2)
eyepiece tube entrance lens) or
specimen
:
too restricted. Open properly. (p 22)

Position correctly so the

Position of condenser too low. vlewfield diaphragm Image IS
formed. (p21 )

Too thick or thin coverglass. Use thickness (0. 17

No coverglass attached. ! mm) coverglass

NCG objective for observing I Use normal objective for

specimen without coverglass used to observmg specimen with
observe specimen with coverglass coverglass.
Normal objective for observing
Poor image
specimen with covergiass used to
obtained. • Use NCG objective
observe specimen without
(Contrast is too lrgla:
strong or too
No Immersion oil used on the front of
weak.) I ImmerSion system objective. Use Nikon ImmerSion all.
(Details are not (p. 25)
clear.)
I Immersion oil used not the type
: specified.
I mmm

i Air bubbles In immersion oil. Remove bubbles. (p25)

Ilmmer:I:~Oil soils the top of dry

system objective (especially 40X)
Cleaning. (p.25)

Compensation ring of objective not

Adjust to match coverglass.
adJusted.
Dirt or dust on the lens (field lens.
condenser, objective, eyepiece.
(p. 2)
eyepiece tube entrance lens) or
specimen. I

27
 v . TROUBLESHOOTING

Failures Causes Actions

Revolving nosepiece not

attached.

Revolving nosepiece not

One side of position.
image is dim.

Image moves
while being
focused.
lens not correctly

Stage tilted.

NCB11 filter not used.

tinged
Turn ON PHOTO voltage
Lamp power source voltage too low, or manipulate brightness
~~... ~.-~...~~-~~~~-.:~ ......~~-~-...~------... ~-~ --~1 adjuster to set the lamp
brightness Indicator scale
too bright. Lamp power source voltage too high. to "9" and adJustthe brightness
using a combination of NO
1--"~-"--"'~"'---r-----"~-"~~"~-------1 filters in the NO filter cassette.
source voltage too low. (p.14 and p.23)

too restricted.
correctly so the
!lAI,AlII",ln diaphragm image is
(p 21)

so that 100%

28
 V. TROUBLESHOOTING

Failures Causes Actions

I Attach to stage with coverglass

up (when no coverglass. .
INo focused image \ Slide upside-down.
I'. specimen surface should be..~
; obtained with high I
: power-objectives.
I
r--- --.. ~-.
. Coverglass too thick.
i up).
'--'-I---'-~-'------'
(p.14)
. Use coverglass of specified •

.-"--"---"1
High-power
objective I Slide upside-down
thickness (0.17 mm)

Attach to stage with coverglass

up (when no coverglass,
I

specimen surface should be

touches (p 14)
the specimen,
when changed- Use coverglass of specified
Coverglass too thick.
over from low I thickness (0.17 mm)
power.
Eyepiece diopter not adjusted. adjustment. (p

Insufficient
parfocality of
Eyepiece diopter not adjusted. Diopter adjustment. (p 20)
objective when
changed-over.

Movement of
holder not tightly fastened to
Fasten it tightly.

Travel of stage
limited to one-half Improper attachment of slide holder Shift the attachment position.
length of slide.

interpupillary distance
(p
No cohesion of
adjustment (p.
binocular image.
of right and
(p.
left eyepieces differ

Interpupillary distance not adjusted.

Experiencing eye diopter not adjusted. (p.20)

fatigue.

Inadequate illumination.
(p.23)

29
 v . TROUBLESHOOTING

ELECTRICAL
Failures Causes Actions

Brightness of
lamp does not
I change even
Turn PHOTO voltage sWitch
though PHOTO voltage sWitch IS ON.
OFF. (p. 24) I
brightness
adjuster is
manipulated.

Lamp does not

light even though
switched ON.

Replacement.

Connect power source cord

and not secure. and lamp housing securely
unstable (p.7a ndp.13)
illumination.
holder not firmly fastened. Fasten securely.
Bulb Insufficiently Inserted into the
Positive connection. (p. 7)
socket.
Bulb immediately Use 12V 100W halogen lamp
blown. Bulb used not the one specified. OSRAM HLX 64623 or
c--------i PHILIPS 7724

Input voltage change-over

illumination Changeover Input voltage :
i does not match the power at
change-over switch. (p. 6)
the room.
Fuse blown. Fuse used not the one specified. Use the fuse specified. {p 6}

30
ELECTRICAL SPECI FICATIONS
Power source ............ 100-120V/220-240V, 50/60Hz
Halogen lamp ............ 12V 100W [use OS RAM HLX64623
or PHILIPS 7724J
Fuse ........................... 100-120V:125V 3.15A
220-240V:250V T2A
Power consumption ... less than 130W

Nikon reserves the right to make such altera-

tions in design as may be considered necessary
in the light of experience. For this reason,
particulars and illustrations in this handbook may
not conform in every detail to models in current
production. 31