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    This document summarizes the results of a COVID-19 RT-PCR test for a 30-year-old male named Mr. RAVULAPALLI PRADEEP. The test was performed on nasopharyngeal and oropharyngeal swabs collected on August 27, 2021. The test results were positive, with N gene and Orf1ab gene Ct values of 20.48 and 23.02 respectively, indicating the presence of SARS-CoV-2. Additional information is provided on Ct value thresholds and the limitations of the COVID-19 RT-PCR test.

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    This document summarizes the results of a COVID-19 RT-PCR test for a 30-year-old male named Mr. RAVULAPALLI PRADEEP. The test was performed on nasopharyngeal and oropharyngeal swabs collected on August 27, 2021. The test results were positive, with N gene and Orf1ab gene Ct values of 20.48 and 23.02 respectively, indicating the presence of SARS-CoV-2. Additional information is provided on Ct value thresholds and the limitations of the COVID-19 RT-PCR test.

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    This document summarizes the results of a COVID-19 RT-PCR test for a 30-year-old male named Mr. RAVULAPALLI PRADEEP. The test was performed on nasopharyngeal and oropharyngeal swabs collected on August 27, 2021. The test results were positive, with N gene and Orf1ab gene Ct values of 20.48 and 23.02 respectively, indicating the presence of SARS-CoV-2. Additional information is provided on Ct value thresholds and the limitations of the COVID-19 RT-PCR test.

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    48 views1 page

    PdfText 938

    Uploaded by

    Chaitanya Chowdary
    This document summarizes the results of a COVID-19 RT-PCR test for a 30-year-old male named Mr. RAVULAPALLI PRADEEP. The test was performed on nasopharyngeal and oropharyngeal swabs collected on August 27, 2021. The test results were positive, with N gene and Orf1ab gene Ct values of 20.48 and 23.02 respectively, indicating the presence of SARS-CoV-2. Additional information is provided on Ct value thresholds and the limitations of the COVID-19 RT-PCR test.

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    Name : Mr.

    RAVULAPALLI PRADEEP Sample ID : 22303774


    Age/Gender : 30 Years/Male Reg. No : 0032108270016
    Referred by : Dr. SELF SPP Code : SPL-STS-161
    Referring Customer : Sage Path Labs -Nalgonda Collected On : 27-Aug-2021 08:09 AM
    ITDOSE INFOSYSTEMS PVT. LTD.

    Primary Sample : Nasopharyngeal and Oropharyngeal swabs Received On : 27-Aug-2021 01:25 PM


    Sample Tested In : Viral Transport Medium Reported On : 27-Aug-2021 04:40 PM
    Client Address : Report Status : Final Report

    MOLECULAR BIOLOGY
    Test Name Results Units Ref. Range Method

    SARS-CoV-2 (COVID-19) RT PCR


    N gene Ct Value 20.48 N/A - Real Time PCR
    Orf1ab gene Ct Value 23.02 N/A - Real Time PCR
    SARS-CoV-2 (COVID-19) Positive N/A Real Time PCR

    ICMR Registration number for Covid -19 is: SAPALAPVLHT


    Ct Value Result
    Negative Negative results indicate the absence of SARS -CoV-2 in the specimen
    Cts<=24 Strong positive reactions indicative of abundant target Viral RNA
    Cts of 25 to
    Positive reactions indicative of moderate amounts of Viral RNA
    31
    Weak reactions indicative of minimal amounts of target nucleic acid
    Cts >=31
    (Viral RNA) which could present an infection state
    What is the threshold cycle or Ct value?:In a real time PCR assay a positive reaction is detected by accumulation of a fluorescent signal. The Ct (cycle
    threshold) is defined as the number of cycles required for the fluorescent signal to cross the threshold (ie exceeds background level). Ct levels are inversely
    proportional to the amount of target nucleic acid in the sample (ie the lower the Ct level the greater the amount of target nucleic acid in the sample).

    Ct Values differ from kit to kit, lab to lab, Collection Process and transportaion conditions and other factors.
    1. COVID-19 detection requires identifying 3 genes; however 2 genes sepcific to SARS-CoV 2 (COVID-19) are sufficient for a positive detection.
    2. E-gene gene is for the detection of Sarbecovirus (Genus B-betacoronavirus) as a first line test. RdRp gene or N-gene are for detection of severe acute respiratory
    syndrome coronavirus 2 (SARS-CoV-2) and are the confirmatory tests.
    Limitations of the Test: Performance of 2019-nCoV Real-Time RT-PCR Diagnostic Panel has only been established in upper and lower respiratory specimens
    (nasopharyngeal or oropharyngeal swabs, sputum, lower respiratory tract aspirates, bronchoalveolar lavage, and nasopharyngeal wash/aspirate or nasal aspirate).

    Correlate Clinically.

    *** End Of Report ***

    Page 1 of 1

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