Abstracts: Abstracts of Papers That Were Published in The IFSCC MAGAZINE, Volume 9, No 3, 2006

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International Journal of Cosmetic Science, 2007, 29, 63–66

Abstracts
Abstracts of papers that were published in the IFSCC MAGAZINE,
Volume 9, No 3, 2006

Formation of Lipid Emulsions and properties of liquid crystal emulsification,


Clear Gels by Liquid Crystal which can be used for oils of various polarity
Emulsification and different molecular constituents. When a
T. Suzuki and H. Iwai self-organizing artificial stratum corneum
Kao Corporation, Global R&D, 2-1-3 Bunka, lipid containing pseudo-ceramide was used as
Sumida-ku, Tokyo 131, Japan a principal component of the oil, a multila-
mellar emulsion of concentric lamellar struc-
Recently developed emulsion technologies for ture was formed. The multilamellar emulsion
the formation of fine emulsions, lipid emul- supplements the physiological function of
sions and clear gels by liquid crystal emulsifi- stratum corneum by the identical mechanism
cation were reviewed. As a basic information as natural intercellular lipids. High-pressure
on liquid crystal emulsification, the structures treatment of the lipid emulsion produced a
and characteristic behaviours of lyotropic gel-like emulsion crystal, in which the homo-
liquid crystals were summarized. Formation geneous nanoemulsion droplets were
of a liquid crystalline phase was often seen in arranged in a hexagonal array.
emulsions and biological systems. The signifi- This review paper was presented at the
cance of liquid crystal formation during Conference of the Asian Societies of Cosmetic
emulsification was analysed by comparing Scientists 2005 in Bangkok.
the states and stabilities of emulsions pre-
pared by different processes. Then uses of
liquid crystals for formation of the character- Prebiotic Cosmetics: An Alternative
istic emulsions and gels were also discussed. to Antibacterial Products
In liquid crystal emulsification, an oil phase D. Bockmühl, C. Jassoy, S. Nieveler, R.
is dispersed directly into the lamellar liquid- Scholtyssek, A. Wadle and M. Waldmann-Laue
crystalline phase composed of surfactant, Henkel KGaA, 40191 Düsseldorf, Germany
glycerol and water to prepare a gel-like oil-
in-liquid crystal emulsion. This is followed by The concept of prebiotics is well known from
dilution with the remaining water to produce food products, where several ingredients are
an emulsion. From the phase behaviour dur- used to stimulate the beneficial gut microflo-
ing emulsification and analysis of the local ra. However, prebiotic substances can in
motion of the liquid crystal membrane by principle be applied to balance almost any
fluorometry, it was confirmed that the inter- microbial community to achieve advanta-
action between surfactant and a polyol mole- geous effects. Since many cosmetic products
cule such as glycerol promotes hydrogen target skin bacteria as a cause for undesirable
bonding and enhances the strength of the conditions like inflammation or body odour,
lamellar liquid crystal membranes, which prebiotic actives can help to cope with these
results in the formation of oil-in-liquid crystal problems in a very effective way. To ensure a
emulsions. The interaction between the liquid focused and successful mode of action we
crystal and oil was analysed from the chan- demonstrated that it is essential to analyse
ges in molecular motion of the membrane at the microflora and the interactions between
the oil-liquid crystal interface using the spin different bacterial species carefully. These
label technique of electron spin resonance analyses were made using a molecular
(ESR). The fluidity of the liquid crystal mem- method, fluorescence in situ hybridization
brane did not change when oil was added, (FISH), allowing a precise determination of a
and therefore oil-in-liquid crystal emulsions bacterial community without the drawbacks
of various oils were prepared by the identical of classical culture methods. In a further step
process. This lack of dependence of the liquid it was then possible to identify new actives
crystal membrane on oil results in the unique that inhibit harmful or unwanted bacterial

Ó 2007 Society of Cosmetic Scientists and the Société Française de Cosmétologie 63


Abstracts

species while protecting the beneficial flora. packaged product to panellists. The given
Using a quick and reliable in vitro screening product was applied to the face twice a day
method we found several plant extracts (e.g. for 8 weeks. Instrumental measurements of
Ginseng or Black currant) which inhibit the the skin colour (a* and L* parameter), skin
inflammation-causing bacterium Propionibac- capacitance, skin tensile properties (R0, R2,
terium acnes, but do not affect beneficial spe- R6), skin thickness and skin microrelief
cies like coagulase-negative staphylococci. In parameters (Ra and Rz) were taken at base-
addition, it could also be demonstrated that a line and after 4 and 8 weeks of use. Clinical
prebiotic product line for treatment of and subjective evaluations were also carried
inflamed (or acne prone) skin showed the out at each control visit. Moreover, the
predicted effects on the microflora composi- amount of cream consumed during usage
tion in a first in vivo trial, i.e. decrease of was calculated by weighing the given jars at
Propionibacterium acnes at a constant level of each visit. Statistical analysis of the data
total bacteria. After 3 weeks, the microflora obtained showed a significant improvement
of 91% of the volunteers had been rebalanced in all instrumental parameters for both
in this way. Furthermore, these formulations groups (‘fancy’ and ‘plain’ packaging),
have been shown to exhibit excellent skin although no significant differences were
compatibility compared to an antibacterial detected between the two groups at any time.
product. Thus, prebiotic substances have the The improvement was higher in the ‘plain’
potential to provide a gentle and sustainable group for most instrumental parameters
alternative to undirected antibacterial ingre- except for micro-relief parameters where the
dients which can cause irritation, and fre- outcome was better in the ‘fancy’ group. Pro-
quently only show short time effects. motion of facial muscle relaxation deriving
Paper presented at the IFSCC Conference from the application of the fancy-packaged
2005, Florence, Italy. cream could be a possible explanation for this
finding. Also the product’s efficacy and sen-
sory properties were perceived with a higher
Objective Evaluation of the Placebo positive impact in the fancy group. The better
performance of the plain packaging group
Effect in Cosmetic Treatments.
could be related to daily application of the
A Randomized Controlled Study ‘proper’ amount of cream. In fact, this group
F. Distante, V. Pagani1, A. Bonfigli1, L. Rigano consumed a larger amount of cream than the
1
and J. Fluhr2 ‘fancy’ group. These results show that a true
1
ISPE – Institute of Skin and Product placebo effect cannot be induced by pack-
Evaluation, Via Bruschetti, 1, 20125 Milan, aging characteristics, although they can
Italy, 2Department of Dermatology, University slightly influence the degree of the expected
of Jena, 07743 Jena, Germany skin benefits in different ways. Moreover,
proper daily dosage of the product appears to
A product’s packaging and claimed efficacy be a key factor in improving the biophysical
may stimulate pleasant emotions during the skin properties related to anti-ageing and
use of cosmetics, thus enhancing their per- restoring effects.
ceived benefits. The aim of this study was to Paper presented at the IFSCC Conference
evaluate if smart packaging and strongly 2005, Florence, Italy.
claimed efficacy attributes can influence the
objectively measured efficacy, allowing a true
placebo effect to be associated with a given
cosmetic treatment. A marketed anti-aging Hydroxy Dimethoxybenzyl Malonate:
gluconolactone-based formulation was selec- A Novel Anti-(Photo)ageing Concept
ted for the study and packaged both in a T. Rudolph, P. Bühle, J. Beck, F. Pflücker,
fancy sophisticated jar and in an unbranded K.-A. Reiffen and H. Buchholz
plain container. Thirty-six female volunteers Merck KGaA, Frankfurter Strasse 250, 64293
(age range: 30–55 years) with facial photo- Darmstadt, Germany
ageing were selected for the study and ran-
domly assigned to the fancy or plain Ideally, anti-(photo)ageing concepts should
packaged product. Pre-agreed messages provide maximum efficacy, perfect stability
aimed at extolling the product’s cosmetic and cosmetic (formulation) elegance. The
effects accompanied delivery of the fancy- need for high efficacy paired with excellent

64 Ó 2007 International Journal of Cosmetic Science, 29, 63–66

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