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    2D Gel Electrophoresis

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    Nandhini D P
    Two-dimensional gel electrophoresis is a technique used to analyze complex protein mixtures. It involves two steps - first, isoelectric focusing separates proteins by their isoelectric points. Second, SDS-PAGE separates them by molecular weight. This results in thousands of protein spots on the gel corresponding to different protein species, and provides information on each protein's properties.

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    2D Gel Electrophoresis

    Uploaded by

    Nandhini D P
    64 views2 pages
    Two-dimensional gel electrophoresis is a technique used to analyze complex protein mixtures. It involves two steps - first, isoelectric focusing separates proteins by their isoelectric points. Second, SDS-PAGE separates them by molecular weight. This results in thousands of protein spots on the gel corresponding to different protein species, and provides information on each protein's properties.

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    2D gel electrophoresis

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    Two-dimensional gel electrophoresis is a technique used to analyze complex protein mixtures. It involves two steps - first, isoelectric focusing separates proteins by their isoelectric points. Second, SDS-PAGE separates them by molecular weight. This results in thousands of protein spots on the gel corresponding to different protein species, and provides information on each protein's properties.

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    © All Rights Reserved
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    Download as doc, pdf, or txt
    64 views2 pages

    2D Gel Electrophoresis

    Uploaded by

    Nandhini D P
    Two-dimensional gel electrophoresis is a technique used to analyze complex protein mixtures. It involves two steps - first, isoelectric focusing separates proteins by their isoelectric points. Second, SDS-PAGE separates them by molecular weight. This results in thousands of protein spots on the gel corresponding to different protein species, and provides information on each protein's properties.

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    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
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    TWO-DIMENSIONAL GEL ELECTROPHORESIS

    INTRODUCTION

    Two-Dimensional Gel Electrophoresis (2-DGE or 2-D electrophoresis) is a powerful and widely


    used method for the analysis of complex protein mixtures extracted from cells, tissues, or other
    biological samples. Two-dimensional electrophoresis was first introduced by P. H. O'Farrell and
    J. Klose in 1975. This technique sorts proteins according to two independent properties in two
    discrete steps: the first-dimension step, isoelectric focusing (IEF), separates proteins acording to
    their isoelectric points (pI); the second-dimension step, SDS-polyacrylamide gel electrophoresis
    (SDS-PAGE), separates proteins according to their molecular weights (Mr, relative molecular
    weight). Each spot on the resulting two-dimensional array corresponds to a single protein species
    in the sample. Thousands of different proteins can thus be separated, and information such as the
    protein pI, the apparent molecular weight, and the amount of each protein is obtained.
    MATERIALS

    1. Lysis Buffer

    2. Rehydration Buffer

    3. Equilibration Buffer

    PROCEDURE

    1. SAMPLE PREPARATION

    2. ISOELECTRIC FOCUSING/THE FIRST DIMENSION

    3. EQUILIBRATION

    4. RUNNING THE SECOND DIMENSION

    5. GEL SILVER STAINING

    6. IMAGE AND DATA ANALYSIS

    RESULT:

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