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    2a, B, C, D, 4d Assignment2

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    Lam Phạm
    The document discusses the results of BLAST and PSI-BLAST searches. A BLAST search of a 759 base pair mitochondrial gene from Ixodes holocyclus matched most closely to accession number AB075955. A PSI-BLAST search using query P04637, an 393 amino acid protein from human and gorilla, identified it as the antigen NY-CO-13 or phosphoprotein p53.

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    2a, B, C, D, 4d Assignment2

    Uploaded by

    Lam Phạm
    15 views1 page
    The document discusses the results of BLAST and PSI-BLAST searches. A BLAST search of a 759 base pair mitochondrial gene from Ixodes holocyclus matched most closely to accession number AB075955. A PSI-BLAST search using query P04637, an 393 amino acid protein from human and gorilla, identified it as the antigen NY-CO-13 or phosphoprotein p53.

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    2a,b,c,d, 4d Assignment2

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    The document discusses the results of BLAST and PSI-BLAST searches. A BLAST search of a 759 base pair mitochondrial gene from Ixodes holocyclus matched most closely to accession number AB075955. A PSI-BLAST search using query P04637, an 393 amino acid protein from human and gorilla, identified it as the antigen NY-CO-13 or phosphoprotein p53.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    15 views1 page

    2a, B, C, D, 4d Assignment2

    Uploaded by

    Lam Phạm
    The document discusses the results of BLAST and PSI-BLAST searches. A BLAST search of a 759 base pair mitochondrial gene from Ixodes holocyclus matched most closely to accession number AB075955. A PSI-BLAST search using query P04637, an 393 amino acid protein from human and gorilla, identified it as the antigen NY-CO-13 or phosphoprotein p53.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 1

    Question 2: .

    Use BLAST to search the sequence below in GenBank with


    the option of NR database

    a) What is the accession number of the closest match to the query sequence?
    AB075955

    b) What is the length of the query sequence?


    759

    c) Is it a nuclear gene or a mitochondrial gene?


    Mitochondrial gene

    d) What is the species identity of the query sequence?


    Ixodes holocyclus

    Question 4: Use P04637 as the query for PSI-BLAST search until no


    new proteins are found, select the nr database & search until no new
    proteins are found.

    b. Type of molecule & its length


    -Type of molecule: amino acid
    -Length: 393
    c. Scientific name & common name of organism
    -Scientific name: +Homo sapiens
    +Gorilla gorilla gorilla

    -Common name: +Taxonomy of human


    +Western lowland gorilla*Based on results of PSI-BLAST:
    d. Identify potential name of this protein.
    Antigen NY-CO-13 or Phosphoprotein p53

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