Fenolik

Operating Time
Time Absorbansi Rata-Rata 0.68 Operating

Absorbansi
1 2 3
0.67 Operating time DP
0.66
10 0.695 0.663 0.649 0.669 0.66
0.65
20 0.640 0.632 0.628 0.633 0.64

Absorbansi
0.62 0.64
30 0.624 0.621 0.619 0.621 0.6 0.63
40 0.625 0.621 0.620 0.622 0.58 0.62
50 0.624 0.62 0.619 0.621 0
0.61 10 20 30 40
60 0.624 0.621 0.62 0.622 0.6 Waktu inkubasi
0.59
0 10 20 30
Waktu ink

Panjang gelombang asam galat

Pengukuran 1 2 3 Rata-rata
Panjang gelombang 724.6 724.6 724.6 724.6 Kadar fenolik total dengan panjang gelom
ABS 0.942 0.942 0.940 0.941 Sampel Abs 1
Metanol 0.740
N-Heksan 0.558
Air 0.494
Kurva baku asam galat Etil Asetat 0.475
Konsentrasi Abs 1 Abs 2 Abs 3 Rata-rata Klorofom 0.373
5 0.348 0.347 0.347 0.347
10 0.456 0.457 0.454 0.456
15 0.571 0.572 0.572 0.572
20 0.636 0.637 0.636 0.636
25 0.745 0.743 0.744 0.744

0.8
Abs 1 Asam galat 0.8 Abs 2 Asam
0.7 f(x) = 0.01944 x + 0.2596
0.7 f(x) = 0.01948 x + 0.259 R² = 0.99291245239236
R² = 0.993432103109279 0.6
0.6
0.5 0.5

0.4 0.4
0.3 0.3
0.2 0.2
0.1 0.1
0 0
0 5 10 15 20 25 30 0 5 10 15
0.2
0.1
0
0 5 10 15
 .68 Operating Time Asam Galat
Operating time DPPH
.67
.66
Kadar fenolik total Abs 1
.65
.64
Metanol 24.67
.63 N-Heksan 15.33
.62 Air 12.05
0.61 10 20 30 40 50 60 70 Etil Asetat 11.08
0.6 Waktu inkubasi Klorofom 5.85
.59 Kadar fenolik total Abs 2
0 10 20 30 40 50 60 70 Metanol 24.85
Waktu inkubasi N-Heksan 15.46
Air 12.16
Etil Asetat 11.08
k total dengan panjang gelombang=724,6 Nm Klorofom 5.98
Abs 2 Abs 3 Kadar fenolik total Abs 3
0.741 0.743 Metanol 24.92
0.559 0.558 N-Heksan 15.44
0.495 0.494 Air 12.15
0.474 0.475 Etil Asetat 11.18
0.375 0.374 Klorofom 6.00

Abs 2 Asam galat Abs 3 Asam galat

0.8
f(x) = 0.01944 x + 0.2596
R² = 0.992912452392362 0.7 f(x) = 0.01952 x + 0.2578
R² = 0.992895500483639
0.6
0.5
0.4
0.3
0.2
0.1
0
5 10 15 20 25 30 0 5 10 15 20
 0.2
0.1
0
5 10 15 20 25 30 0 5 10 15 20
 Kadar fenolik total perberat sampel Abs 1 Sampel Nilai SDS
Metanol 246.7 Metanol 0.1
N-Heksan 153.3 N-Heksan 0.1
Air 120.5 Air 0.1
Etil Asetat 110.8 Etil Asetat 0.1
Klorofom 58.5 Klorofom 0.1
Kadar fenolik total perberat sampel Abs 2
Metanol 248.5
N-Heksan 154.6 Sampel Rata-rata kadar total
Air 121.6 Metanol 24.8
Etil Asetat 110.8 N-Heksan 15.4
Klorofom 59.8 Air 12.1
Kadar fenolik total perberat sampel Abs 3 Etil Asetat 11.1
Metanol 249.2 Klorofom 5.9
N-Heksan 154.4
Air 121.5
Etil Asetat 111.8
Klorofom 60.0

bs 3 Asam galat
2 x + 0.2578
5500483639

15 20 25 30
15 20 25 30
 Kadar fenolik perberat sampel
Metanol 248.1
N-Heksan 154.1
Air 121.2
Etil Asetat 111.1
Klorofom 59.4

a-rata kadar total

kosentrasi abs 1 ABS Control 1 % inhibisi 1
1 0.610 0.741 17.679 ABS 1 Aktivitas
vitamin c
2 0.543 0.741 26.721

%Penghambatan
80.000
3 0.433 0.741 41.565
60.000
4 0.385 0.741 48.043 f(x) = 10.1484480
40.000 R² = 0.986904196
5 0.313 0.741 57.760 20.000
0.000
kosentrasi abs 2 ABS Control 2 % inhibisi 2 0.5 1 1.5 2 2.5 3
1 0.611 0.743 17.766 Konsentrasi (p
2 0.542 0.743 27.052
3 0.431 0.743 41.992
4 0.386 0.743 48.048
5 0.309 0.743 58.412 ABS 2 Aktivitas a
vitamin c
70.000

%Penghambatan
kosentrasi abs 3 ABS Control 3 % inhibisi 3 60.000
50.000 f(x) = 10.228802
1 0.611 0.742 17.655 40.000 R² = 0.98642639
2 0.543 0.742 26.819 30.000
3 0.434 0.742 41.509 20.000
10.000
4 0.387 0.742 47.844 0.000
0.5 1 1.5 2 2.5
5 0.315 0.742 57.547

IC 50 IC 50 rata-rata SD Konsentr
4.151 4.151 0.026413850019
4.113
4.163 ABS 3 Aktivitas a
vitamin c
70.000
nilai rata-rata absorbansi sampel rata-rata %Penghambatan 60.000
50.000 f(x) = 10.080862
0.610 0.611 0.611 0.611 40.000 R² = 0.98677954
0.543 0.542 0.543 0.543 30.000
20.000
0.433 0.431 0.434 0.433 10.000
0.385 0.386 0.387 0.386 0.000
0.313 0.309 0.315 0.312 0.5 1 1.5 2 2.5 3
Konsentrasi
 ABS 1 Aktivitas antioksidan
vitamin c
80.000
60.000
f(x) = 10.1484480431849 x + 7.90823211875845
40.000 R² = 0.986904196800044
20.000
0.000
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5
Konsentrasi (ppm)

ABS 2 Aktivitas antioksidan

vitamin c
70.000
60.000
50.000 f(x) = 10.228802153432 x + 7.96769851951549
40.000 R² = 0.986426390321545
30.000
20.000
10.000
0.000
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5

Konsentrasi (ppm)

ABS 3 Aktivitas antioksidan

vitamin c
70.000
%Penghambatan

60.000
50.000 f(x) = 10.0808625336927 x + 8.03234501347708
40.000 R² = 0.986779541446208
30.000
20.000
10.000
0.000
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5
Konsentrasi (ppm)
 % P e n g h a m b a ta n
kosentrasi ABS Control 1 ABS 1 etil asetat % inhibisi 1
1 0.741 0.675 8.907
2 0.741 0.636 14.170
3 0.741 0.555 25.101
4 0.741 0.527 28.880 ABS 1 Aktivitas antioksid
50.000 fraksi etil asetat
5 0.741 0.417 43.725 40.000
f(x) = 8.43454790823212 x
30.000 R² = 0.963459451460142

kosentrasi ABS Control 2 ABS 2 etil asetat % inhibisi 2 20.000

1 0.743 0.676 9.017 10.000

0.000
2 0.743 0.637 14.266 0.5 1 1.5 2 2.5 3 3.5
3 0.743 0.593 20.188 Konsentrasi (ppm)
4 0.743 0.528 28.937
5 0.743 0.417 43.876
ABS 3 Aktivitas antioks
kosentrasi ABS Control 3 ABS 3 etil asetat % inhibisi 3 50.000fraksi etil asetat
45.000
1 0.742 0.676 8.895

%Penghambatan
40.000
2 0.742 0.640 13.747 f(x) = 8.827493261455
35.000 R² = 0.9667254028914
3 0.742 0.592 20.216 30.000
25.000
4 0.742 0.501 32.480 20.000
5 0.742 0.418 43.666 15.000
10.000
IC50 iC 50 RATA2 SD 5.000
6.064 6.0 0.062013288065 0.000
5.948 0.5 1 1.5 2 2.5 3 3.5
5.968 Konsentrasi (pp

nilai rata-rata absorbansi sampel rata-rata

0.675 0.676 0.676 0.676
0.636 0.637 0.640 0.638
0.592 0.593 0.592 0.592
0.527 0.528 0.528 0.528
0.417 0.417 0.418 0.417
 50.000
ABS 2 Aktivitas antioksidan
45.000 fraksi etil asetat

%Penghambatan
40.000
ABS 1 Aktivitas antioksidan 35.000 f(x) = 8.43876177658143 x − 2.05921938088829
fraksi etil asetat 30.000 R² = 0.948972645726921
25.000
f(x) = 8.43454790823212 x − 1.14709851551956
R² = 0.963459451460142 20.000
15.000
10.000
5.000
0.000
1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5
Konsentrasi (ppm)
Konsentrasi (ppm)

ABS 3 Aktivitas antioksidan

00fraksi etil asetat
00
00
f(x) = 8.82749326145553 x − 2.68194070080862
00 R² = 0.966725402891445
00
00
00
00
00
00
00
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5

Konsentrasi (ppm)
1938088829

5.5
 % P e n g h a m b a ta n
kosentrasi ABS Control 1 ABS 1 N-H % inhibisi 1
1 0.741 0.712 3.914
2 0.741 0.687 7.287
3 0.741 0.663 10.526 ABS 1 Aktivitas antioksidan
4 0.741 0.558 24.696 35.000 fraksi n heksan
30.000
5 0.741 0.495 33.198 25.000
f(x) = 7.59784075573549 x − 6.86909581
R² = 0.926105884415356
20.000

kosentrasi ABS Control 2 ABS 2 N-H % inhibisi 2 15.000

10.000
1 0.743 0.710 4.441 5.000
2 0.743 0.688 7.402 0.000
3 0.743 0.665 10.498 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5

% P e n g h a m b a ta n
Konsentrasi (ppm)
4 0.743 0.560 24.630
5 0.743 0.495 33.378

kosentrasi ABS Control 3 ABS 3 N-H % inhibisi 3

ABS 3 Aktivitas ant
1 0.742 0.703 5.256 fraksi n heksan
2 0.742 0.691 6.873 40.000

3 0.742 0.666 10.243 30.000

f(x) = 7.41239892183
20.000 R² = 0.900051176463
4 0.742 0.559 24.663 10.000
5 0.742 0.494 33.423 0.000
0.5 1 1.5 2 2.5 3 3.5
IC50 iC 50 RATA2 SD Konsentrasi (ppm
7.485 7.5 0.0453215675344
7.518
7.575

nilai rata-rata absorbansi sampel rata-rata

0.712 0.710 0.703 0.708
0.687 0.688 0.691 0.689
0.646 0.665 0.666 0.659
0.560 0.560 0.559 0.560
0.481 0.495 0.494 0.490
 ABS 2 Aktivitas antioksidan
fraksi n heksan
40.000

%Penghambatan
ktivitas antioksidan
heksan 30.000
f(x) = 7.51009421265141 x − 6.46029609690443
59784075573549 x − 6.86909581646424 20.000 R² = 0.91822868130183
26105884415356

10.000
0.000
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5

2 2.5 3 3.5 4 4.5 5 5.5 Konsentrasi (ppm)

sentrasi (ppm)

ABS 3 Aktivitas antioksidan

fraksi n heksan
0.000
0.000
f(x) = 7.41239892183288 x − 6.14555256064688
0.000 R² = 0.900051176463587
0.000
0.000
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5
Konsentrasi (ppm)
 kosentrasi ABS Control 1 ABS 1 klorofom % inhibisi 1
1 0.741 0.550 25.776 ABS 1 Aktivitas antioksi
2 0.741 0.537 27.530 50.000
fraksi klorofom
3 0.741 0.502 32.254

%Penghambatan
40.000 f(x) = 4.50742240215925 x +
4 0.741 0.467 36.977 R² = 0.965785919589985
30.000
5 0.741 0.418 43.590
20.000

kosentrasi ABS Control 2 ABS 2 klorofom % inhibisi 2 10.000

1 0.743 0.550 25.976 0.000
2 0.743 0.535 27.995 0.5 1 1.5 2 2.5 3 3.5 4 4.5

3 0.743 0.502 32.436 Konsentrasi (ppm)

4 0.743 0.468 37.012
5 0.743 0.419 43.607
ABS 3Aktivitas antiok
kosentrasi ABS Control 3 ABS 3 klorofom % inhibisi 3 fraksi klorofom
50.000
1 0.742 0.551 25.741

%Penghambatan
2 0.742 0.539 27.358 40.000 f(x) = 4.7574123989218
R² = 0.95991895972637
3 0.742 0.507 31.671 30.000
4 0.742 0.452 39.084 20.000
5 0.742 0.418 43.666 10.000
IC50 iC 50 RATA2 SD
0.000
6.723 6.6 0.154696220743 0.5 1 1.5 2 2.5 3 3.5
6.748
6.468 Konsentrasi (pp

nilai rata-rata absorbansi sampel rata-rata

0.550 0.550 0.551 0.550
0.537 0.535 0.539 0.537
0.502 0.502 0.507 0.504
0.467 0.468 0.452 0.462
0.418 0.419 0.418 0.418
 ABS 1 Aktivitas antioksidan ABS 2 Aktivitas antioksidan
0
fraksi klorofom 50.000 fraksi klorofom

%Penghambatan
0 40.000 f(x) = 4.42799461641992 x + 20.1211305518169
f(x) = 4.50742240215925 x + 19.7031039136302
R² = 0.965785919589985 30.000 R² = 0.968270297348553
0
20.000
0
10.000
0 0.000
0 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5
Konsentrasi (ppm)
Konsentrasi (ppm)

ABS 3Aktivitas antioksidan

fraksi klorofom
0
0 f(x) = 4.75741239892183 x + 19.2318059299191
R² = 0.959918959726374
0
0
0
0
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5

Konsentrasi (ppm)
 % P en g h a m b a ta n
kosentrasi ABS Control 1 ABS 1 metanol % inhibisi 1
1 0.741 0.713 3.779
2 0.741 0.665 10.256
3 0.741 0.583 21.323
ABS 1 Aktivitas antioksidan
4 0.741 0.500 32.524 ekstrak metanol
5 0.741 0.42 43.320 50.000
40.000 f(x) = 10.1349527665317 x − 8.164642375
R² = 0.992072231173528
kosentrasi ABS Control 2 ABS 2 metanol % inhibisi 2 30.000
20.000
1 0.743 0.712 4.172 10.000
2 0.743 0.664 10.633 0.000
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5
3 0.743 0.584 21.400
Konsentrasi (ppm)
4 0.743 0.505 32.032
5 0.743 0.422 43.203
ABS 3 Aktivitas antioksidan
kosentrasi ABS Control 3 ABS 3 metanol % inhibisi 3 ekstrak metanol
50.000
1 0.742 0.711 4.178

%Penghambatan
40.000 f(x) = 10.0673854447439 x − 7.884097035
2 0.742 0.665 10.377
30.000 R² = 0.990129034089699
3 0.742 0.586 21.024
20.000
4 0.742 0.500 32.615
10.000
5 0.742 0.420 43.396
0.000
IC50 iC 50 RATA2 SD 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5
5.742 5.8 0.022999523484
Konsentrasi (ppm)
5.786
5.754

nilai rata-rata absorbansi sampel rata-rata

0.713 0.712 0.711 0.712
0.665 0.664 0.665 0.665
0.583 0.584 0.586 0.584
0.545 0.546 0.544 0.545
0.423 0.422 0.420 0.422
 ABS 2 Aktivitas antioksidan
ekstrak metanol
50.000
Aktivitas antioksidan

%Penghambatan
metanol 40.000 f(x) = 9.94616419919246 x − 7.55047106325706
30.000 R² = 0.992023788763914
1349527665317 x − 8.16464237516868
2072231173528 20.000
10.000
0.000
2 2.5 3 3.5 4 4.5 5 5.5 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5
nsentrasi (ppm) Konsentrasi (ppm)

Aktivitas antioksidan
k metanol

.0673854447439 x − 7.88409703504042
90129034089699

5 2 2.5 3 3.5 4 4.5 5 5.5

onsentrasi (ppm)
 kosentrasi ABS Control 1 ABS 1 air % inhibisi 1
1 0.741 0.491 33.738
2 0.741 0.483 34.818 ABS 1 Aktivitas antioksid
3 0.741 0.479 35.358 50.000 fraksi air

%Penghambatan
4 0.741 0.470 36.572 40.000
f(x) = 1.9838056680162 x + 30.7
5 0.741 0.424 42.780 30.000 R² = 0.770319406815913
20.000
kosentrasi ABS Control 2 ABS 2 air % inhibisi 2 10.000
1 0.743 0.483 34.993 0.000
2 0.743 0.478 35.666 0.5 1 1.5 2 2.5 3 3.5 4
3 0.743 0.477 35.801 Konsentrasi (ppm)
4 0.743 0.468 37.012
5 0.743 0.416 44.011

kosentrasi ABS Control 3 ABS 3 air % inhibisi 3 ABS 3 Aktivitas antioksid

50.000 fraksi air
1 0.742 0.485 34.636 45.000

%Penghambatan
40.000
2 0.742 0.478 35.580 35.000
f(x) = 2.06199460916442 x + 31
R² = 0.75875145857643
3 0.742 0.476 35.849 30.000
25.000
4 0.742 0.463 37.601 20.000
5 0.742 0.416 43.935 15.000
10.000
IC50 iC 50 RATA2 SD 5.000
9.728 9.4 0.339567209076 0.000
0.5 1 1.5 2 2.5 3 3.5 4
9.452
9.052 Konsentrasi (ppm)

nilai rata-rata absorbansi sampel rata-rata

0.491 0.483 0.485 0.486
0.483 0.478 0.478 0.480
0.479 0.477 0.476 0.477
0.470 0.468 0.463 0.467
0.424 0.416 0.416 0.419
ABS 1 Aktivitas antioksidan ABS 2 Aktivitas antioksidan
50.000 fraksi air
fraksi air

%Penghambatan
40.000
f(x) = 1.93808882907133 x + 31.6823687752355
f(x) = 1.9838056680162 x + 30.7017543859649 R² = 0.680940496519111
30.000
R² = 0.770319406815913
20.000
10.000
0.000
0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 0.5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5

Konsentrasi (ppm) Konsentrasi (ppm)

ABS 3 Aktivitas antioksidan

fraksi air
f(x) = 2.06199460916442 x + 31.3342318059299
R² = 0.75875145857643

5 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5

Konsentrasi (ppm)
 Flavonoid
Operating Time

Absorbansi
Absorbansi
Time Rata-rata
1 2 3 Oprating t
0.78
10 0.777 0.779 0.678 0.745 0.76
20 0.768 0.766 0.765 0.766 0.74
30 0.695 0.695 0.695 0.695 0.72
40 0.695 0.695 0.696 0.695 0.7
50 0.695 0.698 0.695 0.696 0.68
0.66
60 0.697 0.696 0.695 0.696
0.64
0 10 20
W

Panjang gelombang kuarsetin

Pengukuran 1 2 3 Rata-rata
Panjang Gelombang 436.0 436.0 436.0 436.0 Kadar Flavonoid total dengan pan
ABS 0.742 0.742 0.740 0.741 Sampel
Etil asetat
Metanol
Kurva baku kuarsetin klorofom
Konsentrasi Abs 1 Abs 2 Abs 3 Rata-rata N heksan
10 0.311 0.310 0.300 0.307 Air
20 0.452 0.453 0.453 0.453
30 0.596 0.598 0.598 0.597
40 0.688 0.687 0.687 0.687
50 0.842 0.842 0.841 0.842

Kurva Abs 1 Flavanoid Kurva Abs 2 Flavanoi

0.9 0.900
0.8 0.800 f(x) = 0.01298 x + 0.1886
f(x) = 0.01298 x + 0.1884
0.7 R² = 0.995248244378704 0.700 R² = 0.994418802308973
0.6 0.600
Absorbansi

Absorbansi

0.5 0.500
0.4 0.400
0.3 0.300
0.2 0.200
0.1 0.100
0 0.000
5 10 15 20 25 30 35 40 45 50 55 5 10 15 20 25 30 35 40
Konsentrasi (ppm)
Konsentrasi (ppm)

Kurva Abs 3 Flavanoid

0.900
0.800 f(x) = 0.01316 x + 0.181
 Kurva Abs 3 Flavanoid
0.900
0.800 f(x) = 0.01316 x + 0.181
0.700 R² = 0.993408086050519
0.600
Absorbansi

0.500
0.400
0.300
0.200
0.100
0.000
5 10 15 20 25 30 35 40 45 50 55
Konsentrasi (ppm)
 0.78 Oprating time kuarsetin
0.76
0.74
0.72
0.7
0.68
0.66
0.64
0 10 20 30 40 50 60 70
Waktu inkubasi

Flavonoid total dengan panjang gelombang = 436,0 Nm Kadar flavonoid total Abs 1
Abs 1 Abs 2 Abs 3 Etil asetat 43.46
0.753 0.741 0.743 Metanol 28.46
0.558 0.559 0.550 klorofom 23.23
0.490 0.496 0.494 N heksan 22.08
0.475 0.470 0.475 Air 14.00
0.370 0.375 0.374 Kadar flavonoid total Abs 2
Etil asetat 42.54
Metanol 28.54
klorofom 23.69
N heksan 21.69
Air 14.38
Kadar flavonoid total Abs 3
Etil asetat 43.23
Metanol 28.38
klorofom 24.08
va Abs 2 Flavanoid N heksan 22.62
Air 14.85
(x) = 0.01298 x + 0.1886
R² = 0.994418802308973

KORELASI FLAVANOID DAN IC50

50
45
40
35
IC 50 (ppm)

15 20 25 30 35 40 45 50 55 f(x) = − 8.31238361266294 x + 83.3854283054003

30
Konsentrasi (ppm) R² = 0.840036276907951
25
20
15
10
5
0
5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0
 35

IC 50 (ppm
30 f(x) = − 8.31238361266294 x + 83.3854283054003
R² = 0.840036276907951
25
20
15
10
5
0
5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0

kadar total flavanoid (mgEAG/g)

 Kadar flavonoid total perberat sampel abs 1 Sampel Nilai SDS
Etil asetat 434.6 Etil asetat 0.480
Metanol 284.6 Metanol 0.077
klorofom 232.3 klorofom 0.424
N heksan 220.8 N heksan 0.464
Air 140.0 Air 0.424
Kadar flavonid total per berat sampel abs 2
Etil asetat 425.4 Sampel Rata-rata kadar total
Metanol 285.4 Etil asetat 43.08
klorofom 236.9 Metanol 28.46
N heksan 216.9 klorofom 23.67
Air 143.8 N heksan 22.13
Kadar flavonid total per berat sampel abs 3 Air 14.41
Etil asetat 432.3
Metanol 283.8 Sampel ic 50
klorofom 240.8 Etil asetat 5.8
N heksan 226.2 Metanol 6.0
Air 148.5 klorofom 6.6
N heksan 7.5
Air 9.4

N IC50
KORELASI FLAVANOID DAN IC50
50
45
40
4283054003 35
IC 50 (ppm)

30 f(x) = − 6.03817504655494 x + 68.9795158286778

25 R² = 0.693208535827377
20
15
10
5
5 10.0 0
5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0
 40
4283054003 35

IC 50 (ppm)
30 f(x) = − 6.03817504655494 x + 68.9795158286778
25 R² = 0.693208535827377
20
15
10
5
5 10.0 0
5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0
)
kadar total flavanoid (mgEAG/g)
 Kadar flavonoid perberat sampel
Etil asetat 430.77
Metanol 284.62
klorofom 236.67
N heksan 221.28
Air 144.10

rata rata
43.1 43.08
28.5 28.46
23.7 23.67
22.1 22.13
6.1 14.41

50

6778

5 10.0
6778

5 10.0
 Fenolik
Operating Time
Time Absorbansi Rata-Rata 0.68 Operating

Absorbansi
0.67
1 2 3
0.66
10 0.695 0.663 0.649 0.669
0.65
20 0.640 0.632 0.628 0.633 0.64
30 0.624 0.621 0.619 0.621 0.63
40 0.625 0.621 0.620 0.622 0.62
50 0.624 0.62 0.619 0.621 0.61
60 0.624 0.621 0.62 0.622 0.6
0.59
0 10 20 30
Waktu ink

Panjang gelombang asam galat

Pengukuran 1 2 3 Rata-rata
Panjang gelombang 724.6 724.6 724.6 724.6 Kadar fenolik total dengan panjang gelom
ABS 0.942 0.942 0.940 0.941 Sampel Abs 1
Etil Asetat 0.790
Klorofom 0.755
Metanol 0.700
Kurva baku asam galat N-heksan 0.699
Konsentrasi Abs 1 Abs 2 Abs 3 Rata-rata Air 0.406
5 0.348 0.347 0.347 0.347
10 0.456 0.457 0.454 0.456
15 0.571 0.572 0.572 0.572
20 0.636 0.637 0.636 0.636
25 0.745 0.743 0.744 0.744

ABS 1 Asam galat ABS 2 Asam galat

0.8 0.8
0.7 f(x) = 0.01948 x + 0.259 0.7 f(x) = 0.01944 x + 0.2596
0.6 R² = 0.993432103109279 0.6 R² = 0.992912452392362
Absorbansi

0.5
Absorbansi

0.5
0.4 0.4
0.3 0.3
0.2 0.2
0.1 0.1
0
0
0 5 10 15 20 25 30
0 5 10 15 20 25 30
Konsentrasi (ppm)
Konsentrasi (ppm)
.68 Operating Time Asam Galat
.67
.66
Kadar fenolik total Abs 1
.65
.64
Etil Asetat 27.95
.63 Klorofom 26.11
.62 Metanol 23.21
.61 N-heksan 23.16
0.6 Air 7.74
.59 Kadar fenolik total Abs 2
0 10 20 30 40 50 60 70 Etil Asetat 28.00
Waktu inkubasi 25.84
Klorofom
Metanol 23.32
N-heksan 23.05
k total dengan panjang gelombang=724,6 Nm Air 7.53
Abs 2 Abs 3 Kadar fenolik total Abs 3
0.791 0.790 Etil Asetat 28.05
0.750 0.755 Klorofom 26.21
0.702 0.702 Metanol 23.42
0.697 0.6900 N-heksan 22.79
0.402 0.400 Air 7.53

KORELASI
30.0
25.0 f(x) = − 5.2
R² = 0.895

IC 50 (ppm)
20.0
15.0
10.0
5.0
sam galat ABS 3 Asam galat 0.0
0.8 5.5 6.0 6.5
0.7
944 x + 0.2596
0.6
f(x) = 0.01952 x + 0.2578 kadar tot
912452392362 R² = 0.992895500483639
Absorbansi

0.5
0.4
0.3
0.2
0.1
0
0 5 10 15 20 25 30
15 20 25 30
Konsentrasi (ppm)
rasi (ppm)
 Kadar fenolik total perberat sampel Abs 1 Sampel Nilai SDS
Etil Asetat 279.5 Etil Asetat 0.053
Klorofom 261.1 Klorofom 0.190
Metanol 232.1 Metanol 0.105
N-heksan 231.6 N-heksan 0.190
Air 77.4 Air 0.122
Kadar fenolik total perberat sampel Abs 2
Etil Asetat 280.0
Klorofom 258.4 Sampel Rata-rata kadar total
Metanol 233.2 Etil Asetat 28.00
N-heksan 230.5 Klorofom 26.05
Air 75.3 Metanol 23.32
Kadar fenolik total perberat sampel Abs 3 N-heksan 23.00
Etil Asetat 280.5 Air 7.60
Klorofom 262.1
Metanol 234.2 Sampel ic 50
N-heksan 227.9 Etil asetat 5.8
Air 75.3 Metanol 6.0
klorofom 6.6
N heksan 7.5
Air 9.4
KORELASI FENOLIK DAN IC50
30.0
KORELASI FENOLIK DAN IC50
25.0 f(x) = − 5.27839851024208 x + 59.1054934823091
R² = 0.895597059532268
IC 50 (ppm)

20.0 30.00
15.0 25.00 f(x) = − 5.32078677839851 x + 59.1587546554934
IC 50 (ppm)

10.0 R² = 0.929553207124399
20.00
5.0 15.00
0.0 10.00
5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0 5.00
kadar total fenolik (mgEAG/g) 0.00
5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0
kadar total fenolik (mgEAG/g)
 Kadar fenolik perberat sampel
Etil Asetat 280.00
Klorofom 260.53
Metanol 233.16
N-heksan 230.00
Air 75.96

a-rata kadar total

rata-rata
28.0 28.00
24.5 26.05
26.1 23.32
23.0 23.00
7.6 7.60

NOLIK DAN IC50

9851 x + 59.1587546554934
399

8.0 8.5 9.0 9.5 10.0

(mgEAG/g)
sampel IC 50 (ppm)
vitamin c 4.10
etil asetat 5.80
metanol 6.00
klorofom 6.60 10.00
9.00
n heksan 7.50
8.00
air 9.40
7.00
6.00
5.00
4.00
3.00
2.00
1.00
0.00
vitamin c etil asetat

IC 50 rata rata
vitamin c 4.6
etil asetat 6.3
metanol 6.8
klorofom 6.8
n heksan 7.2
air 10.2
 IC50 dengan metode DPPH
0.00
9.00
8.00
10.00
7.00

IC 50 (ppm)
6.00 8.00
IC 50 5.8
5.00 (ppm) 6.00 4.10
4.00
3.00 4.00
2.00 2.00
1.00
0.00
0.00
vitamin c etil asetat metanol klorofom n heksan air

IC 50 dengan metode ABTS

IC 50 (ppm)
12

10 12
8 10
rata rata 8 6.3
6 6 4.6
4
4
2
2 0

0
vitamin c etil asetat metanol klorofom n heksan air
 9.40
10.00
7.50
IC 50 (ppm)

8.00 6.60
5.80 6.00
6.00 4.10
4.00
2.00
0.00
Column G

Sampel
IC 50 (ppm)

12 10.2
10
6.8 6.8 7.2
8 6.3
6 4.6
4
2
0
Column G

Sampel