User Manual

iPette+ Electronic Pipette

2016-ver.1
CONTENTS
1. Product Overview............................................- 1 -
2. Parts Description.............................................- 3 -
3. Display.............................................................. - 5 -
4. Operation..........................................................- 6 -
5. iPette+ setup................................................ - 14 -
6. Storage........................................................... - 18 -
7. Care and Maintenance.................................- 19 -
8. Calibration and Performance Tests............- 24 -
9. Troubleshooting Guide.................................- 25 -
10. Warranty....................................................... - 27 -
11. Accessory..................................................... - 28 -
1. Product Overview

A versatile, motor-driven pipetting instrument,

iPette+ delivers accurate and precise performance in
liquid handling. Its design and operation is based on the
principle of air displacement and use disposable pipette
tips.
iPette+ has been tested according to ISO8655
quality management standards. In compliance with the
quality control requirements of ISO8655-6 / DIN 12650,
each and every iPette+, fitted with original tip of the
manufacturer, has been tested for gravity with distilled
water (DIN / ISO3696, grade 3) at 22℃.

1.1 Specifications

iPette+ covers a wide range of volume: 0.5 - 10μL；5 -50

μL；30 - 300μL；100 - 1000μL

-1-
Chan Volume Range Increment Tips
nels

1 0.5-10 μL 0.01 μL 10 μL
1 5-50 μL 0.1 μL 200;300;350 μL
1 30-300 μL 1 μL 300;350 μL
1 100-1000 μL 5 μL 1000μL

1.2 Pipette Tip

iPette+ is compatible with most universal tip

brands covering full volume range.
Note! Never use iPette+ without a tip attached.

Contents of Package Include:

● iPette+ electronic pipette X 1
● AC Adapter X 1
● USB cable X 1
● Stand X 1
● User manual X 1
● Calibration card（ISO8655-6 / DIN12650）X 1

-2-
 2. Parts Description
Parameter Knob

Function Wheel

Tip Ejector

Display

Handle

Tip Ejector Collar

Pipette Tip cone

-3-
 Stand Charging Port
Fully charged in approximate 4

hours.

Reset Slot
Restart iPette+ with

the light touch of a pin.

USB-charging Port
Fully charged in approximate

4 hours.

-4-
3. Display
Diluting Mode

Dispensing Mode

Pipetting Mode

Buzzer Battery Indicator

& Settings

Volume Range

Parameter

Unit

Indicating

Power off

-5-
4. Operation

4.1 Power On

Press the Parameter Knob or

about 2 seconds.

4.2 Function Selection

① Fast turn the Function wheel right or

left, then turn the Parameter Knob to
do the function Selecting form the
blowing item:
1: Pipetting(PI)
2: Dispensing(ST)
3: Diluting(DI)
4: Settings

② Clicking Parameter Knob to confirm

after selecting the Item.

-6-
4.3 Pipetting (PI)
4.3.1 Volume Setting

①Fast turn the Parameter Knob.

②’PI’ and volume parameter to be blinking.

③ Turn the Parameter Knob anticlockwise

to increase,turn clockwise to decrease.
Press Parameter Knob toconfirm.

Note!：Do volume range setup in

Aspirating mode only.

-7-
4.3.2 Aspiration

When upward arrow blinks,

press Parameter Knob for
Aspiration.

4.3.3 Dispensing

When downward arrow

blinks, press Parameter
Knob for dispensing.

4.3.4 Mixing
Press Parameter Knob until mixing
activity begins.
The release of Parameter Knob
at any time during the process of
mixing will exit the mixing mode.

-8-
Note! Take the operation of iPette+ (10-1000μL) for the
purpose of illustration.

Three mixing volume modes in iPette+：

① If the pipetting volume is set
larger than 30% of the total volume
range of iPette+, 30% of the
maximum volume of the pipette
will be mixed.
e.g. set volume: 700μL
actual mixing volume: 300μL

② If the pipetting volume is set

less than 30% of the total volume
range of iPette+, and more than its
minimum volume range, 50%of the
set volume will be mixed.

e.g. set volume: 270μL

actual mixing volume: 135μL

-9-
③ If 50% of the set volume is less
than the minimum volume range
of iPette+, the minimum volume will
be mixed.
e.g. set volume: 150μL
actual mixing volume: 100μL

4.4 Dispensing (ST

4.4.1 Dispensing volume setting

① Left and right turn Parameter Knob

to set the one-time dispensing volume.
Pressing Parameter Knob to confirm.

② Left and right turn Parameter Knob

to set the one-time dispensing frequency.
(The image shows, dispensing frequency
is 5 times and each volume is 200μL)

- 10 -
4.4.2 Dispensing

Pressing Parameter Knob to do

liquid dispensing.

Pressing the Parameter Knob again

after dispensing the final step liquid had been dispensing
that use to drain the liquid which left in the Tip possible.
the pipette will return to the aspiration conditions.

- 11 -
4.5 Diluting (DI)

4.5.1 Volume setting

① Left and right turn Parameter Knob

to set the volume of target A.
pressing Parameter Knob to confirm.

② Left and right turn Parameter Knob

to set the volume of target B.
pressing Parameter Knob to confirm.

4.5.2 Diluting
A

① Pressing Parameter Knob to

aspirating target A.

- 12 -
 A
② Remove the Tips from the liquid
and Pressing Parameter Knob to air
aspirating a bit of air.

③ Pressing Parameter Knob to air

aspirating target B. B

④ Pressing Parameter Knob to

dispensing target A and B.

- 13 -
5. iPette+ setup

Turn Function wheel right or left

for various settings. Double press
of Parameter Knob at any time
can exit the settings.

Turn Function wheel in any direction for

the selection of settings.

Include:
① Aspiration speed setting
(three speeds )
② Dispensing speed setting
(three speeds)
③ Buzzer status (ON/OFF)
④ Power off

- 14 -
5.1 Adjustment of Aspiration / Dispensing
Speed

① Use Function Wheel to select

aspiration or dispensing speed
when corresponding signs and
parameters start to blink.

② Turn Parameter Knob

right or left to select among
three speeds(1,2,3).

e.g. Speed 2 is set in the right diagram.

- 15 -
③ Press Parameter Knob
to confirm.

5.2 Buzzer Status

① Use Function wheel to

select Buzzer when the
corresponding sign starts
to blink displaying current
status as ON/OFF.

② Turn Parameter Knob to

the right or left to select
between ON and OFF.

- 16 -
③ Press Parameter Knob
to confirm.

5.3 Power off

① Use Function wheel

to select Power when
the corresponding sign
starts to blink.

② Press Parameter Knob

to switch the pipette off.

iPette+ will self switch off

in 15 minutes being
unused.

- 17 -
6. Storage

· Recommend storing iPette+ electronic pipette on the

stand in vertical position when not in use.
· Please every month to charging for iPette+ if being
unused in long time , make sure there are 50% power in
battery at least.
· During storage periods at constant temperature and
humidity, the recommended temperature range is from
0-40℃ and humidity no more than 80%.

- 18 -
7. Care and Maintenance

By cleaning your iPette+ before and after each use, tip

connector in particular, its functionality and performance
can be ensured.

Note! Check the performance of the pipette every three

months. Performance test is recommended to be carried
out after each in-house care and maintenance.

7.1 Cleaning the Outer Surface

Spay the outer surface with a dedicated pipette
detergent or 75% ethanol. Wipe with lint-free cloth.

Note! Please consult with your nearest distributor for the

cleaning with other detergent or liquids.
ANY WARRANTY WILL, HOWEVER, BE DEEMED AS
VOID IF THE FAULT IS FOUND TO HAVE BEEN
CAUSED BY UNAUTHORIZED CLEANING.

- 19 -
7.2 Sterilizing the Inner Parts

The entire body of iPette+ electronic pipette cannot be

autoclaved. The autoclavable parts include:

Ejector spring

Piston spring

Piston
Seal gasket

O ring

Piston cover

Note! The unidentified parts can never be autoclaved or

disassembled prior to authorization.

- 20 -
7.3 Disassembly Procedures

① Unscrew the tip ejector

collar anticlockwise and
remove it.

NOTE! THIS PART CANNOT

BE AUTOCLAVED.

② Remove the ejector spring.

Unscrew the piston cover
anticlockwise and remove it.

- 21 -
③ Separate the seal gasket and
O ring from piston cover.

NOTE! MINOR PARTS FOR

SAFE KEEPING PLEASE.

④ Separate the remaining

piston from piston spring.

- 22 -
⑤ Clean the above-mentioned parts with 75% ethanol,
or sterilize the parts at 121℃, 1 bar overpressure for 20
minutes.

⑥ Apply an appropriate layer of silicone grease to the O

ring, seal gasket and piston. Reassembling.

Note!
① After autoclaving and reassembling, leave iPette+ to
rest for 12 hours before reuse.
② Calibration is needed after each inner-part sterilization.
For more information, please refer to section ”Calibration
and Performance Tests”.

- 23 -
8. Calibration and Performance Tests

Calibration should take place at 20-25℃, kept constant

within ±0.5℃. A dedicated calibration software will write
calibration values in your iPette+, after the distilled water
has been repeatedly weighed at least five times.
Hardware needed:
● Electronic balance with readability of 0.01 mg
● Distilled water
● Pipette tip in compatible with the volume range of your
iPette+
● X86 or X64 architecture PC pre-loaded with Windows
(XP/Vista / 7/8/10 operating system
Software needed:
● Dedicated calibration software of iPette+
(For more information, please contact with your nearest
distributor)
● iPette+ drive program
Note! If your iPette+ cannot work properly after
calibration, please contact your nearest distributor for
assistance.

- 24 -
9. Troubleshooting Guide

Problem Possible cause Solution

Incompatible tip
Remaining
Plastic self-lubrication of the Use original tip
droplets
tip is not even

Tip is loose Attach the tip firmly

Foreign objects between tip Clean the tip cone and

and connector replace the tip

Clean and grease O ring,

Pipette is contaminated
piston and clean tip cone
Leakage or
Inadequate application of
inadequate
silicone grease to piston or Apply silicone grease
aspiration
O ring

O ring and piston is

Replace O ring
unlocked or broken

Follow the instructions in

Inappropriate operation
the User Manual

- 25 -
 Problem Possible cause Solution

Calibration needed or huge Recalibrate according to

Leakage or difference of density the instructions in the

inadequate compared with water User Manual

aspiration Send the pipette for

Pipette is broken
service

Inadequate

aspiration due liquids leak into the tip Clean and grease O ring

to jammed connector and dry. and piston; clean tip cone

pipette

ejector is

jammed or Tip connector and ejector Clean tip connector and

cannot work are contaminated ejector

properly

- 26 -
10. Warranty

iPette+ electronic pipettes are covered by one-year

warranty against defects in workmanship and materials.
Please contact us or your nearest distributor.
ANY WARRANTY WILL, HOWEVER, BE DEEMED AS
VOID DUE TO NORMAL WEAR AND TEAR OR FOR
OPERATIONS CONTRARY TO THE INSTRUCTIONS
GIVEN IN THIS MANUAL.

Each and every iPette+ has been calibrated and tested

in compliance with ISO8655-6 / DIN12650 (calibration
card included in the contents of delivery package) when
manufactured, ensuring safe and comfortable pipetting.

- 27 -
11. Accessory
11.1 Wall mount
Step 1 ： There are two pieces of adhesive tape on
the wall mount surface. Peel off the release paper of
from the adhesive which one you want to use and
paste the wall mount on the flat and clean surface
where you want to set up the wall mount.

Adhesive

Adhesive

- 28 -
Step2：Insert the iPette+ charging port into the wall
mount connection. Connect the USB cable to a power
supply.

USB connector

- 29 -