Holten Laminair BioSafe 1.2 Cabinet - Service Manual

SERVICE MANUAL
Heto-Holten A/S
BIOSAFE 1.2/1.2NF/1.2BS
ID: 807242
81-68-SM.doc – Revision 1
Page 2 / 21 SERVICE MANUAL BIOSAFE 1.2/1.2NF/1.2BS
Symbols used in this manual
WARNING
Used in case of danger of a serious accident or when documentation needs to be
consulted.
F NOTE
Used to direct attention to a special item.
© Copyright 2001
HETO-HOLTEN A/S
Gydevang 17-19
DK-3450 Allerød
Denmark
Telephone +45 48 16 62 00
Fax +45 48 16 62 97
e-mail [email protected]
Home page: http//www.heto-holten.com

81-68-SM.doc
BIOSAFE 1.2/1.2NF/1.2BS SERVICE MANUAL Page 3 of 21
Table of contents
1. Introduction ................................................................................................... 4
2. Test and adjustment procedures ................................................................. 4
2.1. Leak tests........................................................................................................ 4
2.1.1. Equipment required......................................................................................... 4
2.2. Procedure when using a particle counter ........................................................ 4
2.3. Procedure when using a photometer............................................................... 5
2.4. Air velocity test ................................................................................................ 6
2.4.1. Equipment required......................................................................................... 6
2.4.2. Procedure for air velocity test in unidirectional flow ......................................... 7
2.4.3. Procedure for air velocity test in the work opening .......................................... 7
3. Down flow alarm adjustment........................................................................ 8
3.1. Procedure for low alarm adjustment ................................................................ 9
3.2. Procedure for high alarm adjustment............................................................. 10
3.3. Makeup alarm adjustment ............................................................................. 10
3.3.1. Procedure for makeup alarm adjustment....................................................... 10
4. Decontamination by formaldehyde............................................................ 10
4.1. Procedure ..................................................................................................... 11
5. Dismantling and replacement instructions ............................................... 11
5.1. Fuses ............................................................................................................ 12
5.1.1. Procedure for replacement ............................................................................ 12
5.2. Fluorescent light tube .................................................................................... 12
5.2.1. Procedure for replacement (Biosafe 1.2/1.2 NF) ........................................... 13
5.2.2. Procedure for replacement (Biosafe 1.2 BS) ................................................. 13
5.3. Starter ........................................................................................................... 13
5.3.1. Procedure for replacement (Biosafe 1.2/1.2 NF) ........................................... 14
5.3.2. Procedure for replacement (Biosafe 1.2 BS) ................................................. 14
5.4. Access to internal plenum ............................................................................. 14
5.4.1. Procedure ..................................................................................................... 14
5.5. Filters ............................................................................................................ 15
5.5.1. Procedure for replacement of main filter on BIOSAFE 1.2/1.2 NF ................. 15
5.5.2. Procedure for replacement of exhaust filter on BIOSAFE 1.2/1.2 NF ............ 15
5.5.3. Procedure for replacement of main filter on BIOSAFE 1.2 BS ....................... 16
5.5.4. Procedure for replacement of exhaust filter on BIOSAFE 1.2 BS .................. 16
5.6. Access to the electric compartment............................................................... 17
5.6.1. Procedure ..................................................................................................... 17
5.7. Fans.............................................................................................................. 17
5.7.1. Procedure for replacement of the fan ............................................................ 18
5.8. Controller board PCB .................................................................................... 18
5.8.1. Procedure for exchange of the PCB .............................................................. 18
5.9. Key switch..................................................................................................... 18
5.9.1. Procedure for replacement of key switch....................................................... 18
6. Electric diagram .......................................................................................... 19
7. Setting the auto transformer for the fan .................................................... 20
7.1. Procedure for setting air velocity ................................................................... 20
8. Spare parts .................................................................................................. 21
81-68-SM.doc
1. Introduction
This Service Manual for BIOSAFE 1.2, BIOSAFE 1.2 NF and BIOSAFE 1.2 BS is meant to assist
in performing service. It is to be used in conjunction with the Instruction Manual.
2. Test and adjustment procedures

The test procedures are only given as guide lines. Special rules in different countries may require
specific procedures.
To ensure safe operation the setting of the alarm of BIOSAFE 1.2/1.2 NF/1.2 BS should be
checked/adjusted whenever maintenance/repair has been performed.
2.1. Leak tests
2.1.1. Equipment required
• Aerosol
• Aerosol generator
• Diluent and dilution equipment (if available) and Form 00184151 "Leak test of HEPA filter"
may be requested from the HETO-HOLTEN A/S Service Department.
Further equipment required when using a particle counter
• Optical particle counter.
• Isokinetic sensor.
• Particle analyser.
• Optical particle counter with lower sensitivity 0.3 or 0.5 µm. Maximum 10 % double sequence
of errors. Test aerosol DEHS or similar, for example generated with atomiser.
• Diluent dilution system for the particle analyser is advisable.
Further equipment required when using a photometer
• Optical photometer with lower sensitivity 10-3 µg/l with 0.3µm particle and possibility of
measuring concentrations up to 105 times lower sensitivity.
• Test aerosol EMERY 3004.
• Aerosol generator: One or more nozzle(s) of type Laskin.
• Ratio of size of aerosol from generator: 99 % smaller than 3.0 µm, 50 % smaller than 0.7 µm
and 10 % smaller than 0.4 µm.
2.2. Procedure when using a particle counter

1. Start the cabinet and let it run for at least 15 minutes at normal speed with the front window
in work position.
2. Measure the concentration on the suction side of each module. This indicates workload at
normal run.
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3. Start the aerosol generator. Ensure that the aerosol is sucked into the opening of the filter to
be tested.
4. Connect the generator to the service valve.
5. Measure the concentration of aerosol on the backside of the main filter.
6. Connect dilution equipment if available.
7. Adjust the aerosol generator until a constant value higher than 1x106 per ft3 is exceeded.
Record the measured concentration on the form "leak test of HEPA filter".
8. Connect the particle counter to the Isokinetic sensor and set it to measure the total number
of particles. An acoustic signal is produced each time a particle is measured.
9. Move the probe with a speed of maximum 30 mm/sec keeping it about 25 mm from the area
to be tested.
10. Test the area around the filter packing.
11. Test the entire filter surface.
12. Test the entire cabinet inside the clean area.
13. If particles are measured at a particular point, return to this point to measure the particle
concentration and record the value.
14. Determine the highest concentration on the clean side of the filter. This result is defined as
maximum permeability. Relative permeability is calculated as maximum permeability
concentration measured on the unclean side of the filter.
15. Check that the value for maximum relative permeability is below maximum permissible value.
Typical value 1/10000.
16. Adjustments
• Too high permeability at a particular point or at filters packing Contact customer to arrange
replacement of filter and silicone sealant.
• Too high permeability in the construction. Make a silicone sealant, using the same colour of
silicone as used during construction.
2.3. Procedure when using a photometer

1. Start the cabinet and let it run for at least 15 minutes at normal speed with the front window
in work position.
2. Connect the photometer to the module to be tested.
• Warm up the photometer.
• Connect the photometer to the service valve or close the suction side of the fan, in such a
way that the measuring represents the mean concentration on the backside of the filter.
3. Start the aerosol generator. Ensure that the aerosol is sucked into the opening of the filter to
be tested.
4. Measure and record the concentration of aerosol on the backside of the main filter.
81-68-SM.doc
5. Adjust the aerosol generator until a value of 5µg/l is exceeded. Record the measured particle
concentration on the form "Leak test of HEPA filter".
6. Adjust the photometer to indicate 100 %.
7. Adjust the aerosol generator until a value of 1x106 per ft3 is exceeded. Record the measured
concentration on the form "leak test of HEPA filter".
8. Set the photometer to scan with the connected Isokinetic sensor. An acoustic signal sounds
if particle concentration exceeds 0.01 % or reads below 0.1 %.
9. Move the probe with a speed of a maximum of 30 mm/sec keeping it about 25 mm from the
area being tested.
10. Test the area around the filter packing.
11. Test the entire filter surface.
12. Test the entire filter area by moving the probe with overlapping movements.
13. Test the entire cabinet inside the clean area.
14. If a relative permeability higher than 0.001 % is measured at a particular point, return to this
point to measure the particle concentration and record it.
15. Check that the value for maximum relative permeability is below the maximum permissible
value.
16. Adjustment.
a. Too high permeability at a particular point or at filter packing. Contact customer to arrange
replacement of filter and silicone sealant.
b. Too high permeability in the construction. Make a silicone sealant, using the same colour of
silicone as used during construction.
2.4. Air velocity test

Different ways of calibrating the measuring tools may cause discrepancies in the measuring
results. Ask for the previous calibration test results.
Inaccuracies of positioning the measuring positions may cause minor changes of the values
obtained.
The power supply will also influence the measuring results since the fan will give less air at lower
voltage.
The measuring tool should compensate for the ambient temperature otherwise the results must be
corrected.
2.4.1. Equipment required
• Anemometer (Flow Master).
• Measuring PVC pipe with inside diameter Ø160 mm.
81-68-SM.doc
2.4.2. Procedure for air velocity test in unidirectional flow
1. Position the anemometer on top of a stand 250-mm above the tabletop.
2. Measure the air velocity at the indicated test points shown in figure 1.
Figure 1.
3. Calculate the mean value

x1 + x2 ......xn
xav =
n
4. This value has to be 0.4 m/s +/-10%. As there is hysteresis in the alarm system, the values
x1 ...... xn should be between 0.34 m/s and 0.46 m/s in order to allow to adjust the alarm
values which are 0.32 m/s for low alarm and 0.48 m/s for high alarm.
5. The air velocity below the main filter can be adjusted using the different settings on the
autotransformer. See section 7.
2.4.3. Procedure for air velocity test in the work opening
To gain access to the alarm module PCB, see section 5.6.

1. Position the measuring pipe in the work opening. See figure 2.
Figure 2.
2. To avoid false air in the work chamber, seal off all openings around the measuring pipe with
tape.
81-68-SM.doc
3. Place the anemometer at the end of the Ø160 measuring pipe and measure at the 5 test
points. Figure 3. The average velocity in the work opening must exceed 0.4 m/second.
Ø160
22 wmf
Figure 3.
4. Calculate the mean value = (x1 + x2 + ...+ xn)/n
• Calculate the average velocity Vwork opening = 17 / Vpipe
• where17 = Conversion factor.
• Vpipe = Average velocity of the measurements in the measuring pipe.
• Vwork opening = Calculated mean value of velocity.
Minimum acceptable velocity in the work opening ~ Vmin. = 0.4x17.Vmin is the velocity in the
measuring pipe corresponding to 0.4 m/second in the work opening.
3. Down flow alarm adjustment

Equipment required
• Anemometer (Flow Master).
• Stand.
• Screwdriver made from plastics.
• Variable transformer or paper for covering holes.
81-68-SM.doc
Alarm module PCB
Potentiometers
Make up minimum, red LED
Not used. Yellow LED should be off.
Down flow minimum, red LED
Down flow maximum, Yellow LED
Figure 4.
3.1. Procedure for low alarm adjustment

1. Disconnect from mains.
2. Remove the cover of the front panel (See section 5.6) and you have access to the Alarm
module PCB.
3. Connect to mains but be aware of high voltage in the electrical mounting box.
4. Place the anemometer upon a stand 250-mm above the tabletop.
5. Place the front window in work position.
6. Measure the air velocity in the unidirectional flow as described in section 2.4.
7. Place the anemometer where the minimum air velocity is measured.
8. Adjust the variable transformer. Decrease the air velocity until the air velocity indicator
indicates 0.32 m/sec. Alternative procedure Cover the perforations in the tabletop with paper
until the anemometer reaches 0.32 m/second. The perforated area must be evenly covered
in order not to interrupt the airflow profile.
9. Adjust the potentiometer above the LED, which is lit, while the exhaust kit is mounted.
10. Adjust the vario transformer to 230V. In case the alternative procedure was used, remove the
paper.
11. Observe the low alarm to go out.
12. Repeat step and check the alarm.

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13. Let the fan run at normal speed.
14. Seal the screw of the potentiometer.
3.2. Procedure for high alarm adjustment

1. Follow step 1 to 6 in procedure for low alarm adjustment.
2. Locate the position with the highest down-flow.
3. By increasing the voltage either on the internal autotransformer or on an external variable

transformer, increase the down-flow to 0.48 m/s.
4. Adjust the potentiometer on the Alarm module PCB until the yellow light just lights up. See
the previous section and the illustration above.
5. Decrease the voltage until the high alarm goes off.
6. Readjust the voltage to achieve 0.48 m/s and record the voltage.
7. Let the fan run at normal speed.
3.3. Makeup alarm adjustment

Equipment required
• Measuring tube
• Anemometer
• Screwdriver made from plastics.
• Possibly variable transformer.
3.3.1. Procedure for makeup alarm adjustment

1. Using the measuring tube determine the lowest permissible limit for air velocity 0.4 m/s in the
work opening by reducing the voltage on the variable transformer or by adjustment throttle in
the measuring tube.
2. Adjust the potentiometer for make up alarm so that the red light just lights up.
3. Adjust the variable transformer to 230V and check the alarm to go out.
4. Readjust the voltage to achieve 0.4 m/s and record the voltage.
4. Decontamination by formaldehyde
The purpose of this section is to state guidelines regarding decontamination when using
formaldehyde.
HETO-HOLTEN A/S is unable to guarantee a successful decontamination using this method and it
should be regarded as a general and recommended method.
81-68-SM.doc
This method is not applicable for cabinets with activated charcoal filters installed.
NOTE
F Certification is
formaldehyde.
required in certain countries for decontamination using
4.1. Procedure
1. Prepare the formaldehyde solution and install an evaporator in the safety cabinet.
2. Close the window and install the night cover. Seal all crevices on the front.
3. Connect the exhaust of the cabinet via a flexible duct to the fume hood or any suitable
exhaust system.
4. Sufficient formaldehyde (see below) is evaporated inside the closed cabinet to neutralise
contamination by condensing on all internal surfaces. Start the fan and the evaporation of the
safety cabinet. Ammonia has to be added if the evaporator is prepared to be used for
neutralisation.
5. After all formaldehyde has evaporated let the fan run for about 20 minutes, then turn it off.
6. The formaldehyde is left for at least 5 hours’ influence time.
7. Remove the seal from the front window and let the fan run for about 30 minutes thereby
exhausting any remaining from the decontamination process via the fume hood.
8. Remove all remaining seal used during the process.
9. The cabinet is ready for use.
Decontamination agent:
40-ml formaldehyde 37 % diluted with 40-ml water.
Possible neutralisation agents:
27 ml ammonia 25 % (See procedure for used equipment).
5. Dismantling and replacement instructions
NOTE
F Prior to performing any work in the chamber ensure that the cabinet is disinfected
and/or neutralised (Described in section 4)
81-68-SM.doc
5.1. Fuses
All fuses on BIOSAFE 1.2/1.2 NF/1.2 BS are located behind the plate holding the control panel.
Fuse no. Description Phase L/Zero N Rate Order no.

F1 L 10AT/250V
Mains 00841274
F2 N 5x20mm
F3 L 6.3AT/250V
Outlet 888851096
F4 N 5x20mm
F5 N 6.3AT/250V
Light 888851096
F6 L 5x20mm
F7 N 6.3AT/250V
UV light 888851096
F8 L 5x20mm
F1 and F2
F3 and F4
F5,F6,F7 and F8
Figure 5.
Equipment required
• Phillips screwdriver.
• Screwdriver for slotted screws.
• Fuses: 10AT or 6,3 AT.

5.1.1. Procedure for replacement
2. Get access to the electric compartment. See section 5.6.
3. Remove the fuse and replace it.
4. Connect to mains and control the relevant function.
5. Assemble in reverse order.
5.2. Fluorescent light tube

The procedures for changing light tube and starter are different on the different types of Biosafes.
81-68-SM.doc
Equipment required
• Phillips screwdriver.
• Fluorescent light tube: 36W/83 250 V
5.2.1. Procedure for replacement (Biosafe 1.2/1.2 NF)
2. Open the front window.
3. Take of the protective end caps. See figure 6.
Protective end caps
Figure 6.
4. Replace the tube.
5. Connect to mains and control light function.

5.2.2. Procedure for replacement (Biosafe 1.2 BS)
2. Dismount the front window by unscrewing the 2 screws in each side of the upper part of the
frame.
3. Replace the tube.
5.3. Starter
Equipment required
• Phillips screwdriver
• Starter: FS-U 4-80W
81-68-SM.doc
5.3.1. Procedure for replacement (Biosafe 1.2/1.2 NF)
1. Remove the fluorescent light tube. See section.5.2 and 5.2.1.
2. Replace the starter.

5.3.2. Procedure for replacement (Biosafe 1.2 BS)
1. Remove the fluorescent light tube. See section. 5.2 and 5.2.2.
2. Dismount the internal cover using the 2 screws and be aware of the earth connection.
3. Replace the starter in the left fitting by turning it anti clockwise and pull it out.
5.4. Access to internal plenum

Equipment required
• Screwdriver for slotted screws, large and small.
• Hex key 2,5 mm.

5.4.1. Procedure
1. Get access to the electric compartment. See section 5.6.
2. Disconnect the wires for flow sensors, reed relays and light fixture. See figure 7.
Wire for sensor
Wire for light fixture
Wire for reed relays
Figure 7.
4. Dismount the front cover. 6 screws. (On Biosafe 1.2 BS the front window has to be lifted in
order to get access to 3 lower screws). The front cover is hanging on 2 hooks.
5. Pull the cover out at the lower end and get access to plugs and wires on the rear side of the
electrical mounting box. Pull out all wires and disconnect the plug.
6. Take off the front cover.

81-68-SM.doc
5.5. Filters
F NOTE
The HEPA filter is very delicate. A light touch may damage the filter.
In order to avoid any possible harm, it is recommended that while changing filters the service
technician wears a mask with filter of HEPA quality. (H 13 or better).
The used filters must be placed in special bags for biohazard waste immediately after removal. The
special bags should be handled as toxic material and sent for destruction as the normal toxic waste
from the laboratory. Contact the responsible for the laboratory.
See instruction manual section 16 concerning safety
Equipment required
• Screwdriver for Phillips screws number 2.
• Wrench size 13-mm.
• Soap and water.
• 2 cable and wire ties.
• Nippers
• Filters see section 8.
The procedure for changing filters is different because the internal plenum is different on BIOSAFE
1.2/1.2 NF versus BIOSAFE 1.2 BS.
Prior to changing filters, disconnect from mains and open the front cover following the description
in section 5.4
Check proper function of the front window after reassembling of the front cover.
5.5.1. Procedure for replacement of main filter on BIOSAFE 1.2/1.2 NF
1. Dismount the four bolts in each corner of the filter.
2. Lift the plenum by using the two screws placed inside the cabinet.
3. Replace the filter.
4. Test flow velocity and leakage of filters according to standard.

5.5.2. Procedure for replacement of exhaust filter on BIOSAFE 1.2/1.2 NF
1. Dismount the bolts in each corner of the filter.
2. Remove the pressure chamber.
3. Replace the filter.

81-68-SM.doc
5.5.3. Procedure for replacement of main filter on BIOSAFE 1.2 BS
Press here
Figure 8.
1. Loosen the belts on both sides by pressing the buckle as illustrated.
2. Fasten the triangular eyes at each of the corners in the iron frame.
3. Guide the belts over the 2 pulleys internally in the gables.
4. Lift the plenum by pulling the belts.
5. Dismount the brackets in front of the filter in order to avoid damaging the gasket.
6. Remove the filter. The filter might stick to the cabinet frame. Pressing with a flat hand under
the filter cloth might facilitate removal. In case this is not successful the filter might be
removed using a large flat screwdriver or similar. Be careful not to damage the frame. A
plastic plate may be used for protection.
7. Install the new filter. The installation is facilitated by application of soap water to the gasket.
8. Lower the plenum.
9. Free the triangle eyes from hooks and belts.
10. Reconnect the belts to the lower hook in front.
11. Tighten the belts and lock the cam buckle by lifting up the pawl.
12. Coil the belts and fasten them with the cable and wire ties.
13. Reinstall the front cover.
5.5.4. Procedure for replacement of exhaust filter on BIOSAFE 1.2 BS

1. Loosen the screws in front of the filter in order to remove the rails from the frame.
2. Remove the rails and the screws completely using the size 13-mm wrench.
3. Replace the filter without damaging the gasket.

81-68-SM.doc
4. Tighten the frame by suspending it while the rails are moved in place.
5. Check from above that the filter is positioned correctly with the gasket positioned equally on
all surfaces.
6. Tighten the screw until the gasket has good contact to all sides.
7. Reinstall the front cover.
5.6. Access to the electric compartment

Remove the plate holding the control panel to gain access for alarm setting, fuses and
transformers.
Equipment required
Screwdriver for slotted screws.

5.6.1. Procedure
2. Unscrew the plate holding the control panel.
3. Remove the plate and leave it without stretching the wires. See figure 10.
Figure 10.
5.7. Fans
The cabinet is equipped with a directly driven AC fan designed for long life performance and it is
lifetime lubricated. It has built-on capacitors and may be adjusted by changing voltage on the
transformers in the electric compartment. See section 7.
Equipment required
• Fan
• Phillips screwdriver
• 13-mm fork wrench.

81-68-SM.doc
5.7.1. Procedure for replacement of the fan
1. Open the front cover. See section 5.4
2. Dismantle the wire from the terminal block on the fan.
3. Unscrew the fan from plenum.
4. Exchange the fan.
5. Reassemble in reverse order.
5.8. Controller board PCB

Equipment required
Controller board PCB
5-mm wrench and
Anti-static wristband.
5.8.1. Procedure for exchange of the PCB
• Get access to electric compartment by following the instruction in section 5.6
• Using anti static wristband connected to the frame when the prints are touched, dismantle all
wires and sockets on the print.
• Unscrew the print.
• Reassemble in reverse order.
5.9. Key switch

Equipment required
• Key switch including keys.
• 20-mm wrench or fork wrench.
• Graphite dust (Optionally).

5.9.1. Procedure for replacement of key switch
1. Follows to the procedure for replacement of display PCB.
2. Unscrew the key switch and replace it.
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6. Electric diagram
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7. Setting the auto transformer for the fan

Description
The construction of the autotransformer offers a number of possibilities for voltage supply. The fan
may be readjusted to deliver the desired air volume.
Equipment required
• Phillips screwdriver and
• Small screwdriver for slotted screws.
7.1. Procedure for setting air velocity

1. Always disconnect from mains when the transformers are being adjusted.
2. Dismount the plate with the control panel.
3. Adjust the down flow using the black wire (No. 20) on the autotransformer (ASU 96….) until
the average air velocity is measured according to section 2.4.
4. Find the black wire (No. 21) for reduced velocity and place it in an appropriate terminal in
order to obtain a desired air velocity under the main filter. (50-75 % of normal velocity).
Output.
Normal fan speed Black wire from terminal 20 on terminal

block.
Reduced fan speed Black wire from terminal 21 on

terminal block.
Zero: B
A- A A+ 1 2 3 4
Input 230V. A- 216 203 168 141 115 88
A 243 215 178 150 122 94
Black wire from terminal 2 on
terminal block.
A+ 260 245 190 160 130 100
Zero: B
81-68-SM.doc
8. Spare parts
See Instruction Manual regarding filters.
Spare parts
Item Catalogue number
Manual 1.2/1.2 BS 807229 / 807235
Fan 822458
Transformer 847225
Controller board 849112
Alarm module PCB 849117
Flow sensor 849118
Key switch 841004
IEC connector filter 88856013
Fluorescent light tube 844027
Starter 844086
Fuse 10AT 841274
Fuse 6,3AT 88851096
Front cover GS/NF/BS 58250000 / 58250500 / 58250152
Front window 1.2/1.2 BS 58250002 / 58250154
Gasket (BS model only) 2,5 m x 832441
Set of belts (BS model only) 2 x 973128 + 2 x 973129
Tabletop 970225
Silicone (tube) 88831212
Accessories/Options
Item Catalogue number
Anti blow-back valve Ø200 80050035
Gas valve left side 58251016
Carbon-dioxide valve left side 58251017
Vacuum valve left side 58251018
UV-light placed on night cover 58251011
Night cover 58251010
BS exhaust filter 80050038
Flock filter for fan protection 58251020
Minihelic – Differential pressure drop for main filter 80130001
Support stand (height 76-80 cm) 58251000
Support stand (height 88-92 cm) 58252000
Support base for table installation 58250003
Mains voltage 115V 57240257
Mains frequency 60 Hz 57240258
81-68-SM.doc

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SERVICE MANUAL

Symbols used in this manual

Home page: http//www.heto-holten.com

2. Test and adjustment procedures

2.1. Leak tests

2.1.1. Equipment required

Further equipment required when using a particle counter

• Optical particle counter.

• Diluent dilution system for the particle analyser is advisable.

Further equipment required when using a photometer

• Test aerosol EMERY 3004.

• Aerosol generator: One or more nozzle(s) of type Laskin.

2.2. Procedure when using a particle counter

4. Connect the generator to the service valve.

5. Measure the concentration of aerosol on the backside of the main filter.

6. Connect dilution equipment if available.

10. Test the area around the filter packing.

11. Test the entire filter surface.

12. Test the entire cabinet inside the clean area.

2.3. Procedure when using a photometer

2. Connect the photometer to the module to be tested.

• Warm up the photometer.

6. Adjust the photometer to indicate 100 %.

10. Test the area around the filter packing.

11. Test the entire filter surface.

13. Test the entire cabinet inside the clean area.

2.4. Air velocity test

• Anemometer (Flow Master).

• Measuring PVC pipe with inside diameter Ø160 mm.

2.4.2. Procedure for air velocity test in unidirectional flow

1. Position the anemometer on top of a stand 250-mm above the tabletop.

3. Calculate the mean value

To gain access to the alarm module PCB, see section 5.6.

4. Calculate the mean value = (x1 + x2 + ...+ xn)/n

• Calculate the average velocity Vwork opening = 17 / Vpipe

• where17 = Conversion factor.

• Vpipe = Average velocity of the measurements in the measuring pipe.

• Vwork opening = Calculated mean value of velocity.

3. Down flow alarm adjustment

• Anemometer (Flow Master).

• Screwdriver made from plastics.

• Variable transformer or paper for covering holes.

Alarm module PCB

Make up minimum, red LED

Not used. Yellow LED should be off.

Down flow minimum, red LED

Down flow maximum, Yellow LED

3.1. Procedure for low alarm adjustment

4. Place the anemometer upon a stand 250-mm above the tabletop.

5. Place the front window in work position.

7. Place the anemometer where the minimum air velocity is measured.

11. Observe the low alarm to go out.

12. Repeat step and check the alarm.

13. Let the fan run at normal speed.

14. Seal the screw of the potentiometer.

3.2. Procedure for high alarm adjustment

2. Locate the position with the highest down-flow.

3. By increasing the voltage either on the internal autotransformer or on an external variable

5. Decrease the voltage until the high alarm goes off.