Sustrato [A] mM 1 2 8 20 1 mM

Sustrato [B] mM Velocidad mM s-1 1/B (1/mM) 1/V (s

10 0.54 0.93 2.04 2.68 0.1 1.8519
20 0.99 1.61 3.06 3.73 0.05 1.0101
40 1.7 2.55 4.08 4.64 0.025 0.5882
80 2.66 3.6 4.9 5.28 0.0125 0.3759
Pendiente 16.8616
Intersepción 0.1661
V'máx mM/s 6.019
K' mM 101.489
[A] mM 1

Dobles recíp
Velocidad mM s-1

4 1
Linear (1)
3 2 2.0000
Linear (2)
2 8 1.8000 f(x) = 16.8616327561004 x + 0.16
Linear (8) R² = 0.999998256750505
1
20
1.6000
Linear (20)
0
0 10 20 30 40 50 60 70 80 90
1.4000
Sustrato [B] mM
1.2000
1/V (min/mM)

1.0000 f(x) = 9.11306734617338 x + 0.16

R² = 0.999995666132195

0.8000

0.6000

f(x) = 3.26924972887706 x + 0.16

0.4000 R² = 0.999999718044221
f(x) = 2.10047031224094 x + 0.16
R² = 0.999999547883319
0.2000

0.0000
0 0.02 0.04 0.06 0.08

1/B (1/mM)
 f(x) = 3.26924972887706 x + 0.16
0.4000 R² = 0.999999718044221
f(x) = 2.10047031224094 x + 0.16
R² = 0.999999547883319
0.2000

0.0000
0 0.02 0.04 0.06 0.08

1/B (1/mM)
 2mM 8mM 20mM
1/V (s/mM) Sustrato [A] mM V'máx
1.0753 0.4902 0.3731 1 6.019
0.6211 0.3268 0.2681 2 6.083
0.3922 0.2451 0.2155 8 6.124
0.2778 0.2041 0.1894 20 6.132
9.1131 3.2692 2.1005 PENDIENTE
0.1644 0.1633 0.1631 INTERCEPTO
6.083 6.124 6.132 Vmax (mM/min)
55.430 20.020 12.880 KBA Km (mM)
2 8 20

K' para A cte

Dobles recíprocos
120.000

100.000

(x) = 16.8616327561004 x + 0.166142965981437 80.000

² = 0.999998256750505
K' mM

60.000 Row

40.000

1 mM 20.000
Linear (1 mM)
0.000
2mM 0 5 10 15 20 25
(x) = 9.11306734617338 x + 0.164405335685507 Linear (2mM)
² = 0.999995666132195 8mM
[A] mM
Linear (8mM)
20mM
Linear (20mM)

(x) = 3.26924972887706 x + 0.163297202939147

² = 0.999999718044221
(x) = 2.10047031224094 x + 0.163075960082898
² = 0.999999547883319

02 0.04 0.06 0.08 0.1 0.12

1/B (1/mM)
(x) = 3.26924972887706 x + 0.163297202939147
² = 0.999999718044221
(x) = 2.10047031224094 x + 0.163075960082898
² = 0.999999547883319

02 0.04 0.06 0.08 0.1 0.12

1/B (1/mM)
 1/[A] (1/mM) 1/ V'máx (min/mM)
1 0.16614
0.5 0.16439
0.125 0.16329
0.05 0.16308
0.003218039415
0.16
6.14
0.01976

e V' max para A

6.16
6.14
6.12
6.1
6.08
Row 10
6.06
6.04
6.02
6

20 5.98
25
5.96
0 5 10 15 20 25
Sustrato [B] 10 20 40 80 10
Sustrato [A] Velocidad mM s-1 1/A (1/mM) 1/V (s/
1 0.54 0.99 1.7 2.66 1 1.8519
2 0.93 1.61 2.55 3.6 0.5 1.0753
8 2.04 2.55 4.08 4.9 0.125 0.4902
20 2.68 3.6 4.64 5.28 0.05 0.3731
Pendiente 1.5566
Intersepción 0.2958
V'máx mM/s 3.381
K' mM 5.263
[B] mM 10

V0 Vs [A]
Dobles re
6
2.0000
5 1.8000
Velocidad mM s-1

f(x) = 1.55662063177799 x +
4 10 1.6000 R² = 0.999998584812616
20 1.4000
3
1/V (min/mM)
40 1.2000
2 80 1.0000
f(x) = 0.74103850744942 x +
1 0.8000 R² = 0.993597791146875
0.6000
0 f(x) = 0.392266848460005 x
0 5 10 15 20 25 0.4000
R² = 0.99999983287671
0.2000 f(x) = 0.196387515491728 x
Sustrato [A] mM R² = 0.999999928526892
0.0000
0 0.2 0.4 0.6
1/A (1/mM)
 20 40 80
1/V (s/mM) Sustrato [B] mM V'máx
1.0101 0.5882 0.3759 10 3.381
0.6211 0.3922 0.2778 20 3.774
0.3922 0.2451 0.2041 40 5.123
0.2778 0.2155 0.1894 80 5.448
0.7410 0.3923 0.1964 PENDIENTE
0.2650 0.1960 0.1796 INTERCEPTO
3.774 5.102 5.569 KAB Vmax (mM/min)
2.797 2.001 1.094 Km (mM)
20 40 80

Dobles recíprocos
V'máx versus [B]
f(x) = 1.55662063177799 x + 0.2957778794044 6.000
R² = 0.999998584812616 10
Linear (10) 5.000
20
V'máx (mM/min)

Linear (20) 4.000

f(x) = 0.74103850744942 x + 0.264978540767117 40
R² = 0.993597791146875 3.000
Linear (40)
f(x) = 0.392266848460005 x + 0.195990116571809 80 2.000
R² = 0.99999983287671 Linear (80)
f(x) = 0.196387515491728 x + 0.179561027750049
R² = 0.999999928526892 1.000
0.2 0.4 0.6 0.8 1 1.2
0.000
1/A (1/mM) 0 10 20 30 40 50 60 70 80 90
[B] mM
 1/[A] (1/mM)1/ V'máx (min/mM)
0.1 0.29577
0.05 0.26497
0.025 0.19520
0.0125 0.18355
1.32702093
0.17
5.79
7.68534

sus [B] K' versus [B]

6.000

5.000

4.000

3.000
K'

2.000

1.000

0.000
0 50 60 70 80 90 0 10 20 30 40 50 60 70 80 90

] mM [B] mM
80 90
 Vmáx 5.8 mM/min
Km (AB) 7.69 mM
Km(BA) 0.00000 mM
KA 11.7709
KB 0.0198

A constante
K´(Mm) 101.49 55.43 20.02 12.88
[A] 1.00 2.00 8.00 20.00
KA 14.21 16.43 28.84 53.52

B constante
K´(Mm) 5.26 2.80 2.00 1.09
[B] 10.00 20.00 40.00 80.00
KB 12.11 10.07 12.42 12.48
Mecanismo ordenado