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    Microbiological Assay of Antibiotics: Roll: 06, Semester:7th

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    Bilal Abbas
    This document provides an overview of microbiological assays for antibiotics. It discusses how biological assays work using living cells or tissues. It then explains that microbiological assays specifically use microorganisms like bacteria, yeasts and molds. The document outlines that microbiological antibiotic assays compare the growth inhibition of microbes by measured antibiotic concentrations to that of a standard antibiotic preparation. It describes two main types of these assays: the cylinder-plate method using diffusion through agar, and the turbidimetric or tube method monitoring growth in liquid culture. Finally, it briefly introduces the agar diffusion assay which uses discs to test bacterial sensitivity.

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    Microbiological Assay of Antibiotics: Roll: 06, Semester:7th

    Uploaded by

    Bilal Abbas
    225 views17 pages
    This document provides an overview of microbiological assays for antibiotics. It discusses how biological assays work using living cells or tissues. It then explains that microbiological assays specifically use microorganisms like bacteria, yeasts and molds. The document outlines that microbiological antibiotic assays compare the growth inhibition of microbes by measured antibiotic concentrations to that of a standard antibiotic preparation. It describes two main types of these assays: the cylinder-plate method using diffusion through agar, and the turbidimetric or tube method monitoring growth in liquid culture. Finally, it briefly introduces the agar diffusion assay which uses discs to test bacterial sensitivity.

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    microbiologicalassayofantibiotics-190801085106

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    This document provides an overview of microbiological assays for antibiotics. It discusses how biological assays work using living cells or tissues. It then explains that microbiological assays specifically use microorganisms like bacteria, yeasts and molds. The document outlines that microbiological antibiotic assays compare the growth inhibition of microbes by measured antibiotic concentrations to that of a standard antibiotic preparation. It describes two main types of these assays: the cylinder-plate method using diffusion through agar, and the turbidimetric or tube method monitoring growth in liquid culture. Finally, it briefly introduces the agar diffusion assay which uses discs to test bacterial sensitivity.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    225 views17 pages

    Microbiological Assay of Antibiotics: Roll: 06, Semester:7th

    Uploaded by

    Bilal Abbas
    This document provides an overview of microbiological assays for antibiotics. It discusses how biological assays work using living cells or tissues. It then explains that microbiological assays specifically use microorganisms like bacteria, yeasts and molds. The document outlines that microbiological antibiotic assays compare the growth inhibition of microbes by measured antibiotic concentrations to that of a standard antibiotic preparation. It describes two main types of these assays: the cylinder-plate method using diffusion through agar, and the turbidimetric or tube method monitoring growth in liquid culture. Finally, it briefly introduces the agar diffusion assay which uses discs to test bacterial sensitivity.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
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    Presentation on

    Microbiological Assay Of Antibiotics

    Roll: 06,Semester:7th
    Contents
    • Antibiotics

    • Biological Assay

    • Microbiological Assay

    • Microbiological Assay Of Antibiotics

    • Types of Microbiological Assay Of Antibiotics

    • Agar Diffusion Assay


    Biological assay
    •A bioassay is an analytical method to
    determine concentration or potency of a
    substance by its effect on living cells or
    tissues.
    •Experimental animals include mice, rats,
    rabbits, cats, dogs etc.
    Microbiological Assay
    •A type of biological assay is performed with
    micro-organisms.
    e.g, bacteria, yeasts and molds.
    Microbiological Assay Of Antibiotics
    •The microbiological assay of antibiotics is based
    upon a comparison of the inhibition of growth of
    micro-organisms by measured concentration of
    the antibiotics to be examined with that produced
    by known concentrations of a standard
    preparation of the antibiotic having a known
    activity.
    Types of Microbiological Assay Of Antibiotics

    A. The cylinder-plat(or cup-plate)


    method.
    B. The turbidimetric method.
    A. The cylinder-plate(or cup-plate) method.
    • The cylinder-plate method depends upon diffusion of the
    antibiotic from a vertical cylinder through a solidified agar
    layer in the petri dish or plate to and extend such that
    growth of the added micro- organism is prevented entirely in
    a zone around the cylinder containing a solution of
    antibiotic.
    Description
    B. Turbidimetric or Tube Assay method
    •This method depends upon the inhibition of growth
    of a microbial culture in a uniform solution of the
    antibiotic in a fluid medium that is favourable to its
    rapid growth in the absence of the antibiotic.
    Description
    Agar diffusion assay
    •The disk diffusion test, or agar diffusion test
    is a test of the antibiotic sensitivity of
    bacteria.
    •It uses antibiotic discs to test the extent to
    which bacteria are affected by those
    antibiotics.
    Agar diffusion assay

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