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    Stashenko, 2014

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    Sergio mauricio sergio
    This study analyzed the volatile organic compounds, flavonoid profiles, and antioxidant capacities of three tropical flower species: Brownea macrophylla, Petrea volubilis, and Hibiscus spp. Volatile compounds were sampled from the flowers in vitro and in vivo using headspace solid-phase microextraction gas chromatography-mass spectrometry. Liquid chromatography-mass spectrometry was used to identify flavonoids in the flower extracts and measure antioxidant capacity using the ORAC assay. Several flavonoids including pelargonidin derivatives, cyanidin derivatives, and delphinidin glucoside were identified in the different flower extracts. The Brownea macrophylla, Petrea volubilis, and Hibiscus sp

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    Stashenko, 2014

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    Sergio mauricio sergio
    34 views1 page
    This study analyzed the volatile organic compounds, flavonoid profiles, and antioxidant capacities of three tropical flower species: Brownea macrophylla, Petrea volubilis, and Hibiscus spp. Volatile compounds were sampled from the flowers in vitro and in vivo using headspace solid-phase microextraction gas chromatography-mass spectrometry. Liquid chromatography-mass spectrometry was used to identify flavonoids in the flower extracts and measure antioxidant capacity using the ORAC assay. Several flavonoids including pelargonidin derivatives, cyanidin derivatives, and delphinidin glucoside were identified in the different flower extracts. The Brownea macrophylla, Petrea volubilis, and Hibiscus sp

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    This study analyzed the volatile organic compounds, flavonoid profiles, and antioxidant capacities of three tropical flower species: Brownea macrophylla, Petrea volubilis, and Hibiscus spp. Volatile compounds were sampled from the flowers in vitro and in vivo using headspace solid-phase microextraction gas chromatography-mass spectrometry. Liquid chromatography-mass spectrometry was used to identify flavonoids in the flower extracts and measure antioxidant capacity using the ORAC assay. Several flavonoids including pelargonidin derivatives, cyanidin derivatives, and delphinidin glucoside were identified in the different flower extracts. The Brownea macrophylla, Petrea volubilis, and Hibiscus sp

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    34 views1 page

    Stashenko, 2014

    Uploaded by

    Sergio mauricio sergio
    This study analyzed the volatile organic compounds, flavonoid profiles, and antioxidant capacities of three tropical flower species: Brownea macrophylla, Petrea volubilis, and Hibiscus spp. Volatile compounds were sampled from the flowers in vitro and in vivo using headspace solid-phase microextraction gas chromatography-mass spectrometry. Liquid chromatography-mass spectrometry was used to identify flavonoids in the flower extracts and measure antioxidant capacity using the ORAC assay. Several flavonoids including pelargonidin derivatives, cyanidin derivatives, and delphinidin glucoside were identified in the different flower extracts. The Brownea macrophylla, Petrea volubilis, and Hibiscus sp

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    Flower metabolomics: volatile compound

    profile, antioxidant capacity and LC-MS-


    TOF identification of flavonoids in various
    tropical flowers
    E Stashenko 1, J Mejía 1, LJ Sierra 1, DC Bernal 1, L Gualteros 1, K Carrillo 1, JR Martínez 1

    2014

    DOI: 10.1055/s-0035-1565825

    Volatile organic compounds in Brownea macrophylla, Petrea volubilis and Hibiscus spp. flowers
    were sampled in vitro and in vivo with HS-SPME and analyzed by GC-MS. Flavonoids present in
    flower extracts, were identified with LC-MS and their antioxidant capacity was evaluated with the
    ORAC assay. LC-MS data obtained in positive ionization mode from extracts were used to generate
    possible molecular formulas that satisfied the accurate masses. Fragmentation and mass errors
    were used as selection criteria for structure assignment. Pelargonidin-3,5-glucoside, pelargonidin-
    3-glucoside, 8-p-hydroxybenzyl and peonidin were identified in the B. macrophylla extract.
    Pelargonidin glucuronide, apigenin 7-glucuronide methyl ester, cyanidin-3-O-glucuronide,
    pelargonidin-3-O-(6-acetyl-glucoside), and delphinidin-3-glucoside were found in the P. volubilis
    flower extract. Cyanidin-3-glucoside, cyanidin-3,5-glucoside, delphinidin-3-glucoside, and
    peonidin-3-arabinoside were identified in the Hibiscus spp. extract. ORAC antioxidant capacity
    values of 3460, 670 and (1200 – 4035) micromol Trolox/g of substance, were measured for the B.
    macrophylla, P. volubilis and Hibiscus spp. extracts, respectively. These were higher than the
    values of 500 and 480 micromol Trolox/g of substance observed for the reference antioxidants,
    BHT and α-tocopherol, respectively. B. macrophylla, P. volubilis and Hibiscus spp. flowers were
    obtained from experimental plots at the CENIVAM research center. The plant material was freeze-
    dried (Virtis Advance Plus). Solid-liquid extraction with acidified (1% HCl) ethanol-water (3:1, v/v)
    afforded extracts which were roto-evaporated. An LC-MS with electro-spray ionization (LC-ESI-
    TOF, 1200 – 6210 Agilent Technologies, Palo Alto, CA, USA) was used for extract analysis. The
    ORAC assay was implemented in a 96-well microplate reader (Turner Biosystems Inc., Modulus
    Microplate Reader II).

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