Cover Page

3.75 cm.

………………..……… THESIS TITLE ……………….………

…………………………………………………………
…………………… If the thesis / thematic paper
title contains scientific
nomenclature, use Italic style.

Font: type with Times New Roman in

the size of 14 points with upper case,
bolding and single line spacing.

3.75 cm. 2.5 cm.

Locate at the middle FIRSTNAME LASTNAME
of the page.

Type only the first and family name

without titles, except in the case
where there are titles or ranks of
authority, titles conferred by the
King, or priestly rank.

A THESIS SUBMITTED IN PARTIAL FULFILLMENT

OF THE REQUIREMENTS FOR Type name of degree in full.
THE DEGREE OF XXXXXXXXXXXXXXXXXXXX (XXXXXXXX)
FACULTY OF GRADUATE STUDIES Type field of study in which
MAHIDOL UNIVERSITY your degree will be awarded.
2009 Year of graduation (year that student has
2 Lines space # passed thesis / thematic paper defense):
spedify by year (A.D.)
#
COPYRIGHT OF MAHIDOL UNIVERSITY

2.5 cm.
 Example of Cover Page
3.75 cm.

BIOCHEMICAL RESPONSES TO SODIUM CHLORIDE

STRESS IN ROOT OF SALT TOLERANT AND SALT- SENSITIVE
THAI RICE (ORYZA SATIVAL. SPP. INDICA)

POL. MAJ. TREERIN MONGKOL

3.75 cm. 2.5 cm.

A THESIS SUBMITTED IN PARTIAL FULFILLMENT

OF THE REQUIREMENTS FOR THE DEGREE OF
DOCTOR OF PHILOSOPHY (BIOTECHNOLOGY)
FACULTY OF GRADUATE STUDIES
MAHIDOL UNIVERSITY
2009
#
#
COPYRIGHT OF MAHIDOL UNIVERSITY

2.5 cm.
 Entitled Page
3.75 cm.

Thesis
entitled
………………..……… THESIS TITLE ……………….………
………………………………………………………… Font: type with Times New Roman in
…………………… thebolding
size of 14 points with upper case,
and single line spacing.

Use Italic style, if the thesis / thematic

paper title contains scientific
nomenclature.

Font: type with Times New Roman ……………….…..…….…..………

in the size of 12 points with regular Mr./ Ms./ Mrs. First name Last name
and capital letter for the initial letter Candidate
and single line spacing spacing.

………………………….…..………
Academic Position. First name Last name,
Degree (Filed of Study)
Major advisor
Name of the Thesis / Thematic paper
Advisory Committee Members, the
Program Director of the program, and
the Dean: specify with academic position
and title or rank of authority, titles ……………………….….…..……...
conferred by the King, or priestly rank Academic Position. First name Last name,
and degree held.
Degree (Filed of Study) 2.5 cm.
3.75 cm. Co-advisor

Note: If there are many members, ………………….….….…..………..

place above the name of Dean of FGS. Academic Position. First name Last name,
on the left and in the same line
with the right.
Degree (Filed of Study)
Co-advisor

……………………….….…..…… ………………….….….…..…………
Prof. Patcharee Lertrit, Academic Position. First name Last name,
M.D., Ph.D. (Biochemistry) Degree (Filed of Study) Type name of the degree in full.
Dean Program Director
Faculty of Graduate Studies Xxxxxxxxxxxxxxxxxxxxx Program in
Mahidol University Xxxxxxxxxx xxxxxxx Type field of study in which
Faculty of Xxxxxxxxxxx your degree will be awarded.
Mahidol University
Type name of the Faculty / Institute /
College where the program held.

2.5 cm.
 Example of Entitled Page
3.75 cm.

Thesis
entitled
BIOCHEMICAL RESPONSES TO SODIUM CHLORIDE
STRESS IN ROOT OF SALT TOLERANT AND SALT- SENSITIVE
THAI RICE (ORYZA SATIVAL. SPP. INDICA)

……………….………….…..………
Pol. Maj. Treerin Mongkol
Candidate

……………………….….…..………
Asst. Prof. Kanyaratt Supaibulwatana,
Ph.D. (Plant Biotechnology)
Major advisor
3.75 cm. 2.5 cm

……………………….….…..………
Asst. Prof. Sittiruk Roytrakul,
Ph.D. (Molecular Biology)
Co-advisor

……………………….….…..………
Prof. M.R. Jisnuson Svasti,
Ph.D. (Molecular Biology)
Co-advisor

……………………….….…..……… ……………………….….…..………
Prof. Patcharee Lertrit, Assoc. Prof. Chuenchit Boonchird,
M.D., Ph.D. (Biochemistry) Ph.D. (Molecular Biology)
Dean Program Director
Faculty of Graduate Studies Doctor of Philosophy Program in
Mahidol University Biotechnology
Faculty of Science,
Mahidol University

2.5 cm.
 Approval Page
3.75 cm.

Thesis
entitled Font: type with Times New Roman
in the size of 14 points with upper
……………….…… THESIS TITLE ……………….………
case, bolding and single line spacing.
…………………………………………………… Use Italic style, if the thesis /
……………………………… thematic paper title contains scientific
1 Line space # nomenclature.
was submitted to the Faculty of Graduate Studies, Mahidol University
for the degree of Xxxxxxxxxxxx (Xxxxxxxxx) Type name of the Degree,
on name of field of study.
January 1, 2009

The graduation day is the same day that you pass

the thesis / thematic paper defense exam specify
by month / date / year (A.D.) .
Font: type with Times New Roman
in the size of 12 points with regular ……………….………….…..………
and capital letter for the initial letter Mr./ Ms./ Mrs. First name Last name
and single line spacing.
Candidate
Type name and surname with any
titles, ranks, or religious rank
preceding the name ,titles conferred
Name of the Thesis / Thematic paper by the King, or priestly rank.
Advisory Committee Members, the
………………….…..………………
Program Director of the program, and Academic Position. First name Last name,
3.75 cm. the Dean: specify with academic position Degree (Filed of Study) 2.5 cm.
and title or rank of authority, titles Chair
conferred by the King, or priestly rank
and degree held.

………………….…..………….……
Note: If there are many members, Academic Position. First name Last name,
place above the name of Dean of FGS. Degree (Filed of Study)
on the left and in the same line Member
with the right.

………………….…..……………...… ………………….…..………………..
Academic Position. First name Last name, Academic Position. First name Last name,
Degree (Filed of Study) Degree (Filed of Study)
Member Member
Type name of the
Dean/ Director,
where the
program held.
………………….…..……………...… ………………….…..……………..…
Prof. Patcharee Lertrit, Academic Position. First name Last name,
M.D., Ph.D. (Biochemistry) Degree (Filed of Study)
Dean Dean
Faculty of Graduate Studies Faculty of Xxxxxxxxxxxxx
Mahidol University Mahidol University
Type name of the Faculty /
Institute / College
where the program held.
2.5 cm.
 Example of Approval Page
3.75 cm.

Thesis
entitled
BIOCHEMICAL RESPONSES TO SODIUM CHLORIDE
STRESS IN ROOT OF SALT TOLERANT AND SALT- SENSITIVE
THAI RICE (ORYZA SATIVAL. SPP. INDICA)

was submitted to the Faculty of Graduate Studies, Mahidol University

for the degree of Doctor of Philosophy (Biotechnology)
on
January 1, 2009

……………….………….…..………
Pol. Maj. Treerin Mongkol
Candidate

………………….…..………………
Chalermpol Kirdmanee,
3.75 cm. Ph.D. (Horticaltural Engineering), 2.5 cm.
Chair

……………….…..………….……
Asst. Prof. Kanyaratt Supaibulwatana,
Ph.D. (Plant Biotechnology)
Member

………………….…..……………...… ………………….…..………………..
Prof. M.R. Jisnuson Svasti, Asst. Prof. Sittiruk Roytrakul,
Ph.D. (Molecular Biology) Ph.D. (Molecular Biology)
Member Member

………………….…..……………...… ………………….…..……………..…
Prof. Patcharee Lertrit, Prof. Skorn Mongkolsuk,
M.D., Ph.D. (Biochemistry) Ph.D. (Biological Science)
Dean Dean
Faculty of Graduate Studies Faculty of Science
Mahidol University Mahidol University

2.5 cm.
 2.5 cm.
Acknowledgements Page
3.75 cm.
iii

Use lower case Roman

1 Line space # numerals beginning with
the number iii
ACKNOWLEDGEMENTS
Font: type with Times New
Roman in the size of 10 points
Indent = 2 cm. or # with regular style
1 Tab = 2 cm. 2 Lines space
#
2 cm. Font: type with Times New Roman in
……………………………………………………………..…………….…
the size of 14 points with upper case,
bolding and line spacing: 1.5 line
……………….…………………………………………………………………….….…
………………………………….………………………………………………….….…
…………………………………………………….……………………………….….…
………………………………………………………………………..…………….….
Acknowledgments are statements of gratitude to any individual, institution
and /or agency that provided assistance or cooperation during the course
…….……………………………………………………………………………………
of the thesis / thematic paper. They may be extended to the members of
thesis/thematic paper examination committee, financial sponsor, individual
….………..……………………………….….…………….……………………………
who provided suggestion and information as well as those permitted the use
of writings and research equipment. They should be written in plain academic
……………………...………………………….…….…………………………………
style; avoid using spoken and colloquial language
….…….……………………………..……….…………………………………………
Font: type with Times New Roman in the size of 12 points normal
3.75 cm. and capital letter for the initial letter and letters must be alignment
………………………….…………….…………..……………………………….….…
“Justify ” and line spacing: 1.5 line
2.5 cm.
………….…………………………………………………...………………………….
Note: If you have a great deal of information, you can type in single spaced
format and adjust the size of letter, as long as everything fits on 1 page
…….…………………………………….…….……………………………..……….…
………………………………………………………………….…………….…………
…………………………………………….….…………………………………………
………….…………………………………………………….……………….…………
2 cm. ………………………………………………………………………….….
…………….….………………………………………………...………………………
….…….………………………….………….…….……………………………..………
.………………………………………….………………………….…………….…….
………………..………………………………………………………………
1 Line space #
Name - Surname

2.5 cm.
 Example of Acknowledgements Page 2.5 cm.
3.75 cm.
iii

#
ACKNOWLEDGEMENTS
#
#
The success of this thesis can be succeeded by the attentive support from
…………………………………………………………………….….…………….……
…………………….………………………………………………….….………………
……………………………………….……………………………….….………………
…………………………………………………………..…………….….……...………
……………………………………………………………………………….………..…
…………………………….….…………….……………………………………………
3.75 cm. 2.5 cm.
……...………………………….…….…………………………………….…….………
……………………..……….……………………………………………………………
……….…………….…………..……………………………….….…………….………
…………………………………………...………………………….…….……………..
……………………….…….……………………………..……….……………………..
……………………………………………….…………….…………………………….
.………………………….….…………………………………………………….……..
………………………………….………………….………
I would like to thank the entire respondent who was the sampling in this
study …………………………………………………………………………………….
…….…………………………………………………………………….…………….…
…………………………………………………….….…………………………………
………………….………….…….……………………………..……….
#
Pol. Maj. Treerin Mongkol

2.5 cm.
 Font: type with Times New Roman in the Abstract Page 2.5 cm.
size of 10 points with regular style. 3.75 cm.
Fac. of Grad. Studies, Mahidol Univ. Thesis / iv

The statement “Fac. of Grad.

… THESIS TITLE ……………………. ……………………………………………….
Font: type with Times New Roman in Studies, Mahidol Univ.” should be
the size of 12 points with upper case, presented
……………………………..……………………………………………….…..……..…
and single line spacing. on the left corner and the word
2.5 cm. “Thesis” or Thematic Paper
……………………………………………………………….….………………………
Use Italic style, if the thesis / thematic followed by one space before and
paper title contains scientific
after a slash
……………………………………………………………….….………………………
nomenclature. “ / ”, and page number should be
1 line space on the right coner.
#
FIRSTNAME LASTNAME 49366000 XXXX / X Name of the thesis / Thematic paper
Name of field of study in full. advisory committee members and
#
degree held: type only the first and family
X.X. ( XXXXXXXX) name without title,except in the case
where there are titles or rank of authority,
# Name of the Degree in brief. titles conferred by the King.

THESIS ADVISORY COMMITTEE : ……………..………………, Ph.D.,

…….....…. ………………, Ph.D., ……….....………, Ph.D.

Indent = 2 cm. or #
1 Tab = 2 cm.
2 cm. ABSTRACT
……………..………………..………………………………………………
……………………………………………………..…………….………………………
The Abstract should include: objectives, goals, scope of
3.75 cm. the research, research methodology, research findings and 2.5 cm.
……………………………………………………………….….………………………
meet criteria: accuracy , completeness, precision,
conciseness and clarity.
……………………………………………………………….….………………………
Font: type with Times New Roman in the size of 12 points
……………………………………………………………….….………………………
normal and capital letter for the initial letter and letters must
be alignment “Justify ” and line spacing: 1.5 line.
……………………………………………………………….….…….…………………
Note: If you have a great deal of information, you can type in
…………………………………………………………………………..
2.5 cm. single spaced format and adjust the size of letter, as long as
everything fits on 1 page.
…………………………………………………………………..……….…
…….…………………………………………………………………….…………….…
…….………………………………………….………………………
# not more than 5 words
ํ
KEY WORDS: ……………………… / ……………………… / …………………… /
………………………………………… / ………………………
# The Total numbering of thesis / thematic paper
…….. pages

2.5 cm.
 Example of English Abstract Page 2.5 cm.
3.75 cm.
Fac. of Grad. Studies, Mahidol Univ. Thesis / iv

BIOCHEMICAL RESPONSES TO SODIUM CHLORIDE STRESS IN ROOT OF

SALT TOLERANT AND SALT- SENSITIVE THAI RICE (ORYZA SATIVAL. SPP.
INDICA)
#
POL.MAJ. TREERIN MONGKOL 4936000 SCBT/D
#
Ph.D.(BIOTECHNOLOGY)
#
THESIS ADVISORY COMMITTEE : KANYARATT SUPAIBULWATANA, Ph.D.
SITTIRUK ROYTRAKUL, Ph.D., M.R. JISNUSON SVASTI, Ph.D.
#
ABSTRACT 2.5 cm.
3.75 cm.
……………………………………………………………….….………….
…………………………………………………………………………….….…………
…………………………………………………………………………….….…………
…………………………………………………………………………….….…….……
……………………………………………………………………………..
……………………………………………………………….….………….
…………………………………………………………………………….….…………
…………………………………………………………………………….….…………
…………………………………………………………………………….….…….……
…………………………………………………………………………….….…………
…………………………………………………………………………….….…….……
#
KEY WORDS: RICE / ROOT / SUGARS / BIOCHEMICAL RESPONSES /
PROTEOMICS
#
125 pages

2.5 cm.
 Contents Page 2.5 cm.
3.75 cm.
v
Font: type with Times New
Roman in the size of
1 line space # 10 points with regular style.

CONTENTS
Font: type with Times Font: type with Times New Roman
New Roman in the # in the size of 14 points with
size of 12 points with 2 lines space upper case, bolding and
upper case, bolding and # line spacing: 1.5 line.
line spacing: 1.5 line.

Page
ACKNOWLEDGEMENTS Font: type with Times New iii
Roman in the size of 12 points
ABSTRACT (ENGLISH) with bolding and capital letter iv
for the initial letter .
ABSTRACT (THAI) v
LIST OF TABLES vii
LIST OF FIGURES viii
LIST OF ABBREVIATIONS ix
CHAPTER I INTRODUCTION 1
3.75 cm. CHAPTER II OBJECTIVES ......
2.5 cm.
CHAPTER III …………………………………………. ......
Font: type with
3.1 ……………………………………….…..
Times New Roman ......
in the size of 12
3.1.1 ……………………………….
points with regular ......
style and capital
3.1.2 ……………………………….
letter for the initial ......
letter and line
CHAPTER IV ………………………………….….…….
spacing: 1.5 line. Consult with your Major- ......
advisor to determine the
CHAPTER V ……………………………………..…..... internal arrangement to ......
the chapters and
CHAPTER VI ………………………………….……… reference sections ......
REFERENCES …………………………..……....…….. ......
APPENDICES ......
Appendix A ............................................................................... ......
Appendix B ................................................................................ ......
Appendix C ................................................................................. ......
Appendix D ............................................................................... ......
BIOGRAPHY ......

2.5 cm.
 List of Tables 2.5 cm.
3.75 cm.
Font: type with Times New vi
Roman in the size of 10 points
with regular style.
1 line space #
LIST OF TABLES Font: type with Times New Roman
in the size of 14 points with
2 lines space # upper case, bolding and
line spacing: 1.5 line.
#
Font: type with Times New
Table Roman in the size of 12 points Page
with bolding and capital letter
3.1 for the initial letter .
……………………………………………………….…………… 17
4.1 ………………………………………………….………………… 29
4.2 Font: type with Times New Roman
……………………………………………….…………………… 39
in the size of 12 points
with regular style.
…………………………………………………………………… …..
…………………………………………………………………… …..
……………………………………………………………………
Font: type with Times New Roman …..
in the size of 12 points with regular
……………………………………………………………………
style and capital letter for the initial …..
letter and line spacing: 1.5 line.
3.75 cm. …………………………………………………………………… …..
…………………………………………………………………… ….. 2.5 cm.
…………………………………………………………………… …..
…………………………………………………………………… …..
…………………………………………………………………… …..
…………………………………………………………………… …..
…………………………………………………………………… …..
…………………………………………………………………… …..
…………………………………………………………………… …..
…………………………………………………………………… …..
…………………………………………………………………… …..
…………………………………………………………………… …..
…………………………………………………………………… …..
5.12 …………………………………………………………………… …..
5.13 …………………………………………………………………… …..
5.14 …………………………………………………………………… …..
5.15 …………………………………………………………………… …..
5.16 …………………………………………………………………… …..

2.5 cm.
 List of Tables (cont.) 2.5 cm.
Font: type with
Times New Roman
3.75 cm in the size of 10
points with vii
regular style.

LIST OF TABLES (cont.)

2 lines space # Font: type with Times New
Roman in the size of 14 points
# with upper case, bolding and
line spacing: 1.5 line.
Table Font: type with Times New Roman in Page
the size of 12 points with bolding and
5.17 ……………………………………………………………………
capital letter for the initial letter . …..
5.18 …………………………………………………………………… …..
5.19 …………………………………………………………………… …..
Font: type with Times New Roman
5.20 in the size of 12 points with
…………………………………………………………………… …..
regular style.
6.1 …………………………………………………………………… 86
Font: type with Times New Roman
6.2 ……………………………………………………………………
in the size of 12 points with regular style 87
and capital letter for the initial letter and
6.3 ……………………………………………………………………
line spacing: 1.5 line. 88

3.75 cm. 2.5 cm.

2.5 cm.
 Example of Contents 2.5 cm.
3.75 cm.
v

#
CONTENTS
#
#
Page
ACKNOWLEDGEMENTS iii
ABSTRACT (ENGLISH) iv
ABSTRACT (THAI) v
LIST OF TABLES (if any) vii
LIST OF FIGURES (if any) viii
LIST OF ABBREVIATIONS (if any) ix
CHAPTER I INTRODUCTION 1
CHAPTER II OBJECTIVES 5
3.75 cm.
CHAPTER III MATERIALS AND METHODS 15 2.5 cm.
3.1 Materials Consult 39
with your
3.2 Methods Major-advisor 42
to determine
3.2.1 Culture condition of SK-N-SH cells the internal 42
arrangement
3.2.2 Cell viablity assay to the chapters 43
and reference
CHAPTER IV RESULTS 48
sections
CHAPTER V DISCUSSION 64
CHAPTER VI CONCLUSION 72
REFERENCES 74
APPENDICES 88
Appendix A Instruction of the instruments in this study 99
Appendix B Madia and Reagents 109
Appendix C Mascot Search Results 119
Appendix D Human Subjects Approval Document 123
BIOGRAPHY 125

2.5 cm.
 Example of Tables Page 2.5 cm.
3.75 cm.
vi

#
LIST OF TABLES
#
#
Table Page
3.1 Common types of dementia and their typical characteristics 17
4.1 Types of reactive oxygen species 29
4.2 Compared differences between necrosis and apoptosis 39
……………………………………………………………………
……………………………………………………………………
……………………………………………………………………
……………………………………………………………………
……………………………………………………………………
3.75 cm. 2.5 cm.
……………………………………………………………………
……………………………………………………………………
……………………………………………………………………
……………………………………………………………………
……………………………………………………………………
……………………………………………………………………
……………………………………………………………………
……………………………………………………………………
……………………………………………………………………
……………………………………………………………………
5.13 Cytotoxicity of H2O2 at 24 hours and the protection from mangosteen 65
extract 200 and 400 g/ml by Trypan blue assay
5.14 Cytotoxicity of H2O2 at various exposure times by MTT assay 67
5.15 Cytotoxicity from various concentrations of H2O2 at 24 hours 69
incubation measured by MTT assay
5.16 Cytotoxicity from mangosteen extract on SK-N-SH cells various 70
times by MTT assay

2.5 cm.
 List of Tables (cont.) 2.5 cm.
3.75 cm
vii

LIST OF TABLES (cont.)

#
#
Table Page
5.17 Cytotoxicity screening of mangosteen extract at various concentrations on 72
SK-N-SH cells at 24 hours incubation by MTT assay
5.18 Protective effects from various incubation times of mangosteen 74
extract against H2O2 150, 300 M induced SD-N-SH cells toxicity
measured by MTT assay
5.19 Protective effects from mangosteen extract 25-800 g/ml against 77
H2O2 cytotoxicity at 75, 150 and 300 M on SK-N-SH cells
5.20 The percentages of cell population measured by flow cytometry 80
3.75 cm. 6.1 Presumed caspase-3 activities from the protection of mangosteen 84 2.5 cm.

extract 200 g/ml against H2O2 150 M at 3,6 and 24 hour ixposure
6.2 ROS production decreased from the protection of mangosteen extract 87
200 g/ml against 150-600 M H2O2 induced cell death during 0-180
min exposure
6.3 ROS production after pretreated with mangosteen extract 200 g/ml, 89
vitamin C 25-100 M and vitamin E 5-25 M against 150 M
H2O2exposusre

2.5 cm.
 Font: type with Times New Roman in The Odd Page 2.5 cm.
the size of 10 points with regular style. 3.75 cm.
Fac. of Grad. Studies, Mahidol Univ. Ph.D. (Biotechnology) / 15

The statement “Fac. of Grad. Studies,

# Mahidol Univ.” should be presented on the
1 line space left corner and the degree followed by one
space before and after a slash “ / ”, and page
CHAPTER III number should be on the right corner.
Font: type with Times
New Roman in the size MATERIALS AND METHODS
of 14 points with bolding
and capital letter for #
the initial letter . 2 lines space Font: type with Times New
# Roman in the size of 14 points
with upper case, bolding and
3.1 Heading (1) line spacing: 1.5 line.

2 cm. Indent = 2 cm. or

...........................................................................................................………
1 Tab = 2 cm.
……...................................................................................................................................
...........................................................................................................................................
Font: type with Times New Roman in
................................................................................................................................……...
the size of 12 points with regular style
and capital letter for the initial letter and
..........................................................................................................…………….............
letters must be alignment “Justify ” and
line spacing: 1.5 line.
...........................................................................................................................................
3.75 cm.
........................................................................................................................................... 2.5 cm.
...........................................................................................................…………….......….
..........................................................................................................…
#
2 lines space
#
3.2 Heading (2)
2 cm. ........................................................................................................................
...........................................................................................................................................
...............................................................................................................................………
...........................................................................................................……………............
...........................................................................................................................................
..............................................................................................................................……….
..........................................................................................................…………….............
...........................................................................................................................................
............................................
2 cm. ..............................................................……………......................................
...................………......…………….......…………….......................................................
....................................................................................…...................................................

2.5 cm.
 Font: type with Times New Roman in
the size of 10 points with regular style .
The Even Page 2.5 cm.
3.75 cm.
Pol. Maj. Treerin Mongkol Materials and Methods / 16

Name of student should be

...........................................................................................................................................
presented on the left corner and
name of chapter followed by one
...................………………………………….......………………………..........................
space………………. before and after a slash “ / ”
and page number on the right
...........................................................................................................................................
..............................................................................................................................……….
..........................................................................................................…………….............
...........................................................................................................................................
Font: type with Times New Roman in
the size of 12 points with regular style
...........................................................................................................................................
and capital letter for the initial letter and
letters must be alignment “Justify”
..............................................................................................................................……….
and line spacing: 1.5 line.
.................................................................................................................
# 2 lines space
Font: type with Times New Roman in
the size of 14 points with bolding and
# capital letter for the initial letter .

3.3 Heading (3)

2 cm ........................................................................................................................
Indent = 2 cm. or 2.5 cm.
3.75 cm. 1 Tab = 2 cm.
...............................................................................................................
# 1 line space
Font: type with Times New Roman in
3.3.1 Subheading the size of 12 points with bolding and
capital letter for the initial letter .
2 cm ........................................................................................................................
...............................................................................
#
3.3.2 Subheading
........................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
.........................................................................................................
4 cm. 3.3.2.1 ...................................................................................…..
...........................................................................……………….....
3.3.2.2 .......................................................…………………….
3.3.2.3 .......................................................……………………
.......................................................................................................

2.5 cm.
 Example of the Odd Page 2.5 cm.
3.75 cm.
Fac. of Grad. Studies, Mahidol Univ. Ph.D. (Biotechnology) / 15

#
CHAPTER III
MATERIALS AND METHODS
#
#
3.1  Chemicals and reagents 
Chemicals and reagents used in this study ....................................................
...........................................................................................................................................
...........................................................................................................................................
.......................................................................................................................……………
...........................................................................................................……………............
...........................................................................................................................................
...........................................................................................................................................
............................................................................................................…………….......…
...........................................................................................................……………............ 2.5 cm.
3.75 cm.
#
#
3.2 Plant Materials
Thai rice (Oryza satival. spp. indica) ……………………………………..
...........................................................................................................................................
...........................................................................................................................................
....................................................................................………
...........................................................................................................………
……...................................................................................................................................
...............................................................................................................................………
……...................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
...........................................................................................................................................
.......................................................................................................................………............

...........................................................................................................……………............

2.5 cm.
 Example The Even Page 2.5 cm.
3.75 cm.
Pol. Maj. Treerin Mongkol Materials and Methods / 16

...........................................................................................................................................
...........................................................................................................................................
............................................................................................................…………….......…
...........................................................................................................……………...........
.............................................................................................................
#
#
3.3 Methods
………………………………….......……………………………………….
...........................................................................................................
#
3.3.1 Culture condition of SK-N-SH cells
3.75 cm. 2.5 cm.
………………………………….......……………………………………….
...........................................................................................................
#
3.3.2 Cell viablity assay
........................................................................................................................
...........................................................................................................................................
................................................................................................…………….......…............
...........................................................................................................
3.3.2.1 Determination of morphological cell changes: using
phase contrast inverted microscope
3.3.2.2 MTT assay
3.3.2.3 Determination of reactive oxygen species production
Reactive oxygen species (ROS)………………………………..
........................................................…………………….……………………………......
...................................................................................................…………….............…...
….......................................................................................................................................

2.5 cm.
 Font: type with Times New Roman in References Page
the size of 10 points with regular style 2.5 cm.
3.75 cm.

Fac. of Grad. Studies, Mahidol Univ. Ph.D. (Biotechnology) / 57

1 line space #
REFERENCES
Font: type with Times New
# Roman in the size of 14 points
2 lines space with upper case, bolding and
# line spacing: 1.5 line

……………………………………………………………………………………….......…………………
2 cm. Indent = 2 cm. or
…………………………………………………………...........................................….……
1 Tab = 2 cm.
………………………………………………………………………….…………………
C
…………………………………………………..
……………………………………………………………………………………….......…………………
The references or Bibliography section
…………………………………………………………….…………………………..
is the list of books, Journals, articles, documents
and other information sources that were used in
……………………………………………………………………………………….......…………………
course of writing the thesis. The section serves to
support the quality of the information used.
…………………………………………………………….………………………………
The References or Bibliography will
come after the Main Body and conclusions, but
……………………………………………………….......
3.75 cm. before the appendices. 2.5 cm.
The word “References” is used in the
……………………………………………………………………………….……………………………
Sciences and Technology, while the word
………………………………………………….…………………………………………..
“Bibliography” is used in the social sciences.
The function of both is the same.
……………………………………………………………………………………….......
Font: type with Times New Roman in
……………………………………………………………………………………….......
the size of 12 points with regular style and
capital letter for the initial letter and letters must
……………………………………………………………………………………………………………
be alignment “Justified ” and line spacing: 1.5 line
…………………………………………….
For a Thai Language :
Font: type with Angsana New in
the size of 16 points normal and capital letter for
the initial letter and letters must be alignment
“Justify ” and single line spacing.

2.5 cm.
 Example of References Page
 
2.5 cm.
3.75 Cm.
Biography Page

Fac. of Grad. Studies, Mahidol Univ. Ph.D. (Biotechnology) / 57

#
REFERENCES
#
#
1 Zybailov, B.L., & Washburn, M.P. (2005). Mass spectrometry-based methods of
proteome analysis. In R.A. Meyers (Ed.), Encyclopedia of Molecular Cell
Biology and Molecular Medicine (Vol. 8). Kanasa City, MO, USA: Stowers
Institute for Medical Research.
2 Finney D. Probit analysis, third endition .London: Cambridge University Press 1971
3 Watananonta, W. (2006). Present Situation and its Future Potential of Cassava Production
and Utilization in Thailand. Paper presented at the FEALAC Inter-regional
Workshop on Clean Fuels and Vehicle Technologies: therole of Science and
Innovation.
4 Gakh, O., Cavadini, P., & Isaya, G. (2002). Mitochondrial processing peptidases.
Biochimica et Biophysica et Biophysica Acta 1592 63-77.
5 Chen, J., Wan, J., Jiang, H., Gao, X., Wang, P., Xi, J., et al. (2006). Cloning and Expression
Analysis of OsNADPH1 Gene from Rice in Drought Stress Response. Rice
3.75 cm.
Science, 13(3),149-154. 2.5 cm.

2.5 cm.
 2.5 cm.
Biography Page
3.75 cm.
Fac. of Grad. Studies, Mahidol Univ. Ph.D. (Biotechnology) / 125

#
BIOGRAPHY
#
2 lines space
#
Type name and surname with
NAME ………………...........…………………….
any titles and ranks.

DATE OF BIRTH …………………...........…………………

PLACE OF BIRTH …………………...........…………………...
Province and Country
…………….………………..……………...
…………….………………..……………...
Educational history – degrees held,
institutions attended, year of
INSTITUTIONS ATTENDED .…………………….……….…………...
graduation. Starting with
Bachelor’s degree .
Font: type with Times New ……………………….……….…………...
Roman in the size of 12
points with regular style ……………………….……….…………..
and capital letter for the
initial letter and line ……………………….……….…………..
spacing: 1.5 line.
……………………….……….…………..
Academic awards, scholarships or
SCHOLARSHIP RECEIVED . ……………………….……….…………..
research grants received during study
at Mahidol University (if any)
…………….………………..……………...
3.75 cm. 2.5 cm.
RESEARCH GRANTS …………….………………..……………...
…………….………………..……………...
HOME ADDRESS …………….………………..……………...
…………….………………..……………...
Tel. ………………….……………………..
E-mail : ................…….…………………...
EMPLOYMENT ADDRESS …………….………………..……………...
…………….………………..……………...
Tel. …………….………………..………...
E-mail : ................…….…….……..….…...
AWARD RECEIVED …………….………………..……………...
…………….………………..……………...
PUBLICATION / PRESENTATION …………….………………..……………...
…………………………………..…………

2.5 cm.
 Example of Biography Page 2.5 cm.
3.75 cm.
Fac. of Grad. Studies, Mahidol Univ. Ph.D. (Biotechnology) / 125

#
BIOGRAPHY
#
#
NAME Pol. Maj. Treerin Mongkol
DATE OF BIRTH 7 June 1974
PLACE OF BIRTH Chiang Mai, Thailand
INSTITUTIONS ATTENDED Chiang Mai University, 1997-2000
Bachelor of Science (Biotechnology)
Chiang Mai University, 2000-2002
Master of Science (Biotechnology)
Mahidol University, 2007-2009
Doctor of Philosophy (Biotechnology)
3.75 cm.
2.5 cm.
SCHOLARSHIP RECEIVED . ……………………….……….…………..
RESEARCH GRANTS . ……………………………………………
. ………………….……..…………………
HOME ADDRESS . ………………….….…………………….
. ………………….…………..……………
. ………………….…………..……………
. ………………….…………..……………
EMPLOYMENT ADDRESS …………………………………………......
………………..………….………………..
. ………………….…………..……………
. ………………….…………..……………
PUBLICATION / PRESENTATION ………………….…………………..……..
………………………….…………..……..
…………………………………..…………
……………..………………….…………..
…………………………………..…………

2.5 cm.