Received: 18 December 2017 Revised: 6 July 2018 Accepted: 12 October 2018

DOI: 10.1002/aqc.3010

SHORT COMMUNICATION

Environmental DNA as a non‐invasive sampling tool to detect

the spawning distribution of European anadromous shads
(Alosa spp.)
Caterina Maria Antognazza | J. Robert Britton |Caitlin Potter | Elizabeth Franklin |
Emilie A. Hardouin | Catherine Gutmann Roberts | Miran Aprahamian | Demetra Andreou

Department of Life and Environmental

Sciences, Bournemouth University, Poole, UK Abstract
Correspondence 1. Populations of the European shads Alosa alosa (Linnaeus, 1758) and Alosa fallax
Caterina Maria Antognazza, Bournemouth
Lacépède, 1800 (Alosa spp.) are protected under legislation because of their
University, Fern Barrow, Poole, BH12 5BB,
UK. vulnerability to human disturbances. In particular, river impoundments block
Email: [email protected]
their upstream migration, preventing access to spawning areas. Knowledge on the
Funding information
Heritage Lottery Fund, Grant/Award Number:
spatial extent of their spawning is important for informing conservation and river
HG/15/04573; LIFE Nature Programme, management plans.
Grant/Award Number: LIFE15/NAT/UK/
000219 2. Determining the spatial extent of Alosa spp. spawning is challenging. They enter
rivers over a 2‐3‐month period and the species potentially migrate different
distances upstream. Capture and handling can be problematic, spawning events
generally occur at night, and kick‐sampling for eggs is limited to shallow
water. Assessing their spatial extent of spawning could, however, incorporate
non‐invasive sampling tools, such as environmental DNA (eDNA).
3. An eDNA assay for Alosa spp. was successfully developed, based on the
cytochrome c oxidase subunit I gene segment and quantitative polymerase chain
reaction (qPCR). Application in spring 2017 to the River Teme (River Severn
catchment, western England) revealed high sensitivity in both laboratory and field
trials. Field data indicated Alosa spp. spawning between May and June, with
migrants mainly restricted to areas downstream of the final impoundment.

4. eDNA can thus be used as a non‐invasive sampling tool to determine the

freshwater distribution of these fishes in Europe, enhancing their conservation
at local and regional scales.

KEY W ORDS

detection, environmental DNA, impoundment, migratory fish, monitoring, qPCR

1 | I N T RO D U CT I O N throughout their geographical range (Aprahamian, Aprahamian,

Baglinière, Sabatié, & Alexandrino, 2003), with both species listed in
European shads Alosa alosa (Linnaeus, 1758) and Alosa fallax Lacépède, the Bern Convention (Appendix V) and in the Habitats Directive of the
1800 are cryptic, anadromous fishes, the distributions of which overlap European Union (Annexes II and V) (Aprahamian et al., 2003;
(Alexandrino et al., 2006). In general, their populations have declined Aprahamian, Lester, & Aprahamian, 1999; Council of the European

148 © 2019 John Wiley & Sons, Ltd. wileyonlinelibrary.com/journal/aqc Aquatic Conserv: Mar Freshw Ecosyst. 2019;29:148–152.
ANTOGNAZZA ET AL. 149

Communities, 1992). Where they spawn in close proximity, the fishes restricted to the area below the final impoundment (Powick Weir), close
tend to produce reproductively viable hybrids (Jolly et al., 2012). to the Severn confluence (Pinder et al., 2016). The field trials deter-
The spawning behaviour of these Alosa spp. involves migration mined the duration of the Alosa spawning period and the spatial extent
into fresh water in spring (with the timing dependent on location, of their distribution. The spatial distribution of the fish was assessed to
but usually in April–July; Kottelat & Freyhof, 2007). Of the two enable an assessment of how the partial removal of this impoundment
species, A. alosa tends to migrate the furthest upstream to spawn, so will subsequently affect the spatial distribution of spawning Alosa spp.
when unimpeded the two fishes can segregate their spawning areas; in the river (Environment Agency, 2018).
however, the construction of weirs on many European rivers now
largely prevents this segregation, resulting in high genetic introgres-
sion (Jolly et al., 2012), with A. alosa largely absent from many of its
former rivers (Aprahamian et al., 1999). 2 | METHODS
The conservation of Alosa spp. in European rivers requires spatial
and temporal information on their spawning distributions, and how 2.1 | eDNA filtering and extraction
these relate to river impoundments. Assessments of their spawning
distributions can, however, be difficult to complete using capture Samples were collected at four sites on the River Teme in 2017
methods, owing to the general sensitivity of the fishes to handling and (Table 1). The primary focus was on site 1, located downstream of
anaesthesia (Breine et al., 2017). Egg sampling can provide positive the final weir impoundment, where Alosa spp. have been historically
indications of spawning activity (Caswell & Aprahamian, 2001; observed to spawn, enabling the duration of the spawning season to
JNCC, 2015), but this can be labour intensive when applied across be determined. To assess their spatial distribution, three additional
large spatial areas. It is also limited to areas of relatively shallow waters, sites were used, all upstream of the weir at site 1, at distances of up
with the spawning of Alosa spp. in some European rivers occurring in the to 48 km upstream. Initial samples were collected in March (as
deeper, lower reaches, including estuarine areas (Breine et al., 2017; controls), and then again between late May and early July (Table 1).
Magath & Thiel, 2013). The detection of spawning events can be All water samples were collected in 1‐L sterile plastic bottles.
completed, but these tend to occur at night. An alternative is to use Water samples were collected by two methods; first, by samplers
environmental DNA (eDNA), a non‐invasive sampling tool that has standing in the riparian zone. Sampling bottles were attached to an
increasingly been shown to provide a reliable method for detecting rare extendible pole (1.8–3.7 m). Equipment was cleaned after collecting
and endangered aquatic species (Pilliod, Goldberg, Arkle, & Waits, each sample (with 10% microsol detergent; Anachem, Leicester, UK).
2013). Although there remains some uncertainties in the application Ten water samples were collected per site, comprising paired samples
and interpretation of eDNA data (e.g. Roussel, Paillisson, Treguier, & (at 1.8 and 3.7 m) from five sampling points (at 10‐m intervals). Two
Petit, 2015), evidence increasingly suggests that it can provide greater negative controls were taken: after five samples (1.8 m) and after 10
probabilities of detection of aquatic species when compared with the samples (3.7 m). These were the same type of bottles but filled with
use of traditional sampling techniques (Dejean et al., 2012; Jerde, sterile water and treated in the same manner as the sample collection
Mahon, Chadderton, & Lodge, 2011), especially when ‘best practice’ bottles. The sampling equipment was changed and sterilized between
methods are used (Wilcox et al., 2018). sampling points. The second sampling method collected the samples
The aim of this study was to develop and test an eDNA sampling from bridges, with water from 10 samples and two negative controls
tool for the detection of Alosa spp. in rivers during their spawning migra- initially collected from each bridge across the wetted width of the
tions. A quantitative PCR (qPCR) was developed to detect Alosa spp, and river. This was reduced to five and one negative control following
its utility was tested using laboratory and field trials. The field trials were initial analyses. During sampling, each bottle had been pre‐weighted
carried out on the River Teme, a major tributary of the River Severn, (700 g) and placed individually in a plastic sample bag. In the field, each
western England, where current data suggest that Alosa spawning is bottle was lowered into the river on a rope to collect the sample.

TABLE 1 Description of sampling sites. Site, GPS coordinates, date of sampling, number of water samples collected, and the number of samples
with eDNA detection of Alosa spp. DNA are indicated
Location Site Sampling method GPS coordinates Date Water samples eDNA detection of Alosa spp.

Powick 1 Bridge 52.170497, −2.242295 30/05/17 8 8

12/06/17 10 4
19/06/17 10 6
02/07/17 10 2
18/07/17 5 0
08/08/17 5 0
Riparian zone 52.169564, −2.240533 23/03/17 10 0
30/05/17 10 9
Bransford 2 Bridge 52.176929, −2.288100 30/05/17 10 0
Knightwick 3 Bridge 52.201276, −2.392410 30/05/17 10 0
Tenbury Wells 4 Bridge 52.313900, −2.594711 05/06/17 10 2
18/07/17 5 0
08/08/17 5 0
150 ANTOGNAZZA ET AL.

2.2 | eDNA qPCR assay development dilutions in the late cycle (>37), which corresponded to 0.1 pg μL−1,
were unreliable as the probability of detection was <95%. No amplifi-
The primer and probe specific for the Alosa spp. cytochrome c oxidase cation was detected in all negative controls. The qPCR was also found
subunit I gene segment (COI) was designed by Applied Biosystems to be highly specific to Alosa spp., with no cross‐species amplification
(assay ID: APMFW3H; Applied Biosystems, Foster City, CA). Probe detected.
and primer sequences were designed using European Alosa spp.
(A. alosa, A. fallax, and hybrids) sequences in the National Centre for
3.2 | Comparing eDNA sampling methods
Biotechnology Information nucleotide database (NCBI, https://www.
ncbi.nlm.nih.gov/). Specificity to European Alosa spp. was determined Both water sampling methods resulted in positive detections of Alosa
with an in silico test using target and off‐target species commonly DNA (Table 1). Sampling from the riparian zone resulted in signifi-
found in British fresh waters (Table S1). The TaqMan® Gene Expres- cantly higher Ct values and eDNA concentrations than from bridges
sion Master Mix UDG was used for this assay (Applied Biosystems). (non‐parametric Wilcoxon rank test: Z = −2.59 and Z = −3.39, respec-
DNA extracted from scales of Alosa spp. collected from the River tively; P < 0.05). Bridge sampling was more time efficient in the field,
Severn catchment was used as a template for assay validation and however, as equipment was pre‐prepared and pre‐sterilized in the
standard curves for qPCR. laboratory, and thus was the preferred method.
The Alosa species‐specific COI gene assay was tested for cross‐
reactivity with pure fish DNA present in the freshwater areas of the
3.3 | eDNA detection of Alosa spp.
River Severn catchment (10 ng for each of the following fish species:
Abramis brama (Linnaeus, 1758) (common bream), Alburnus alburnus
Water samples collected from the River Teme in March were negative
(Linnaeus, 1758) (bleak), Anguilla anguilla (Linnaeus, 1758) (eel), Barbus
but were all found to be positive at the end of May. Peak DNA
barbus (Linnaeus, 1758) (European barbel), Cyprinus carpio Linnaeus,
concentrations occurred in mid‐June, and final detections were
1758 (carp), Gobio gobio (Linnaeus, 1758) (gudgeon), Lampetra planeri
recorded in early July (Figure 1; Table 1;). Spatially, Alosa spp. DNA
(Bloch, 1784) (brook lamprey), Leuciscus leuciscus (Linnaeus, 1758)
was most frequently detected at site 1 (Table 1). No positive samples
(dace), Perca fluviatilis Linnaeus, 1758 (perch), Petromyzon marinus
were recorded from sites 2 and 3, but Alosa DNA was detected in two
Linnaeus, 1758 (sea lamprey), Phoxinus phoxinus (Linnaeus, 1758)
water samples in early June at site 4 (Table 1).
(minnow), Rutilus rutilus (Linnaeus, 1758) (roach), Salmo salar Linnaeus,
1758 (Atlantic salmon), Salmo trutta Linnaeus, 1758 (brown trout),
Squalius cephalus (Linnaeus, 1758) (chub), and Thymallus thymallus 4 | DISCUSSION
(Linnaeus, 1758) (grayling). Note that because the eDNA water
samples were being collected from freshwater areas only, An eDNA method to detect the presence of Alosa spp. in rivers was
cross‐reactivity was not tested for other fishes of the Clupeidae family successfully developed and tested. This assay had a discrete level of
that occur in marine and estuarine waters (e.g. Clupea harengus resolution (detection limit: 1 pg μL−1) and high specificity for Alosa spp.
Linnaeus, 1758). The assay was also not tested on North American Temporally, positive samples were recorded between May and early
Alosa spp. (e.g. Alosa sapidissima (A. Wilson, 1811) and Alosa July at site 1, with peak DNA concentrations in mid‐June. Only two
pseudoharengus (A. Wilson, 1811)). To determine the sensitivity of positive samples were recorded further upstream. These initial data
the assay, a calibration curve was generated using genomic DNA thus suggest that the primary spawning area in this river was at site 1,
extracted from the scales of Alosa spp. A 10‐fold serial dilution of downstream of the final weir, with a much smaller number of
Alosa spp. genomic DNA was prepared to give a template concentra- individuals by‐passing this weir and moving further upstream. The
tion from 10 ng μL−1 to 1 fg μL−1. The detection limit was defined spawning activity in site 1 was validated by the presence of Alosa eggs
as the lowest genomic Alosa DNA concentration detected at least
95% of the time by the qPCR assay. qPCR was run for each eDNA
sample in triplicate in 20 μL, under the manufacturer's instructions,
with 2 μL of DNA template (undiluted). The qPCR method used
warm‐up conditions of 50°C for 2 min and 95°C for 10 min, followed
by 40 cycles between 95°C for 15 s and 60°C for 1 min. All negative
controls were performed in triplicate.

3 | RESULTS

3.1 | eDNA assay validation

FIGURE 1 Mean cycle threshold (Ct, black squares) and eDNA
Using a 10‐fold serial solution of Alosa spp. genomic DNA, the limit of concentration (ng μL−1) for Alosa spp. (grey circles) in the River Teme
detection of the assay was 1 pg μL−1, with a mean cycle threshold (Ct) below Powick Weir. Errors around the means represent the 95%
value of 37 (±0.02 SD). The Ct values with standard genomic DNA confidence limits
ANTOGNAZZA ET AL. 151

that were regularly sampled in this section between mid‐May and mid‐ In this study of the River Teme, the results suggested that small
June (unpublished data). numbers of Alosa spp. can occasionally pass the final barrier and move
The detection rates of eDNA can be relatively high in river water as far as 48 km upstream. The planned modification of this impound-
samples (Pilliod et al., 2013), although information on the spatial resolu- ment should thus open up more of the catchment to migrating
tion of these detections often remains uncertain (Goldberg, Strickler, & Alosa spp. than is presently the case (Environment Agency, 2018).
Pilliod, 2015). For example, macroinvertebrate DNA can be detected Subsequent refinement and testing of the assay will enable this to
from source populations up to 10 km upstream (Deiner & Altermatt, be tested and, in general, will improve the power of this assay to
2014). For fish, distances tend to be closer to 1 km upstream assess the temporal and spatial patterns of migrating Alosa spp. in
(Balasingham, Walter, & Heath, 2017). However, the absence of a European rivers.
consistent relationship between eDNA concentration and downstream
distances (Laramie, Pilliod, & Goldberg, 2015) suggest that consistent ACKNOWLEDGEMENTS
DNA accumulations do not occur. This is because of DNA settlement Financial support for this study was given by a studentship from the
on the river bed and subsequent re‐suspension and degradation Severn Rivers Trust and Bournemouth University, with funding from
(Shogren et al., 2017; Wilcox et al., 2016). The positive detections of ‘Unlocking the Severn for LIFE’, LIFE Nature Programme (LIFE15/
Alosa at site 1 were all from samples collected approximately 0.5 km NAT/UK/000219), the Heritage Lottery Fund (HG/15/04573).
downstream of the final impoundment. Consequently, it was assumed
that the DNA was all from fish present downstream of this weir. It ORCID
was less clear where the Alosa spp. detected at site 4 were located,
Caterina Maria Antognazza https://orcid.org/0000-0001-5344-
and further investigation will represent an important step to
8245
understanding this result. Moreover, the general lack of species‐specific
J. Robert Britton https://orcid.org/0000-0003-1853-3086
markers to discriminate between these Alosa species (Faria, Weiss, &
Alexandrino, 2012) meant that it was not possible to determine whether RE FE RE NC ES
this DNA originated from A. alosa, A. fallax, or a hybrid form. Although
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catchment. Correspondingly, the distances from the Severn estuary to
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