IETS 2018 Program Book Final

44th Annual Conference
Shangri-La Hotel • Bangkok, Thailand • January 13–16, 2018
PROGRAM
BOOK
Program Book
44th Annual Conference of the
International Embryo Technology Society
Building an Embryo: A Fertile Journey
Shangri-La Hotel
Bangkok, Thailand
January 13–16, 2018
Scientific Program Co-Chairs:

Trudee Fair and Daniel F. Salamone
Table of Contents
Preface and Acknowledgments......................................................................................................... 1
2018 Recipients of the IETS Pioneer Award.................................................................................... 3
Map of the Venue.............................................................................................................................. 4
General Information.......................................................................................................................... 6
Program............................................................................................................................................. 9
Section Editors and Manuscript and Abstract Reviewers............................................................... 15
Poster Session Information............................................................................................................. 17
Poster Session Order by Topic........................................................................................................ 18
Author Index................................................................................................................................... 34
2018 Recipient of the IETS Distinguished Service Award............................................................. 41
Special Events................................................................................................................................. 42
IETS Foundation 2018 Early Career Achievement Award............................................................. 45
Session Speakers and Keynote Biographies................................................................................... 46
Exhibit Hall Layout......................................................................................................................... 49
Exhibit Directory............................................................................................................................ 50
Morulas Preconference Workshop: Assessment of Mammalian Embryo Quality: Invasive and
Non-Invasive Techniques............................................................................................................. 57
CANDES Preconference Symposium: Monitoring and Controlling Ovarian Activities in
CANDES..................................................................................................................................... 58
Thank You to Our Sponsors............................................................................................................ 59
2017 IETS Board of Governors

Christine Wrenzycki, Immediate Past President
Fulvio Gandolfi, President
Daniel F. Salamone, Vice President
Barbara S. Durrant, Treasurer
Marcelo Bertolini, Governor
Katrin Hinrichs, Governor
Irina A. Polejaeva, Governor
Dimitrios Rizos, Governor
Pablo J. Ross, Governor

Preface and Acknowledgments
The 44th Annual Conference of the International Embryo Technology Society will be held at the Shangri-La
Hotel, Bangkok, Thailand, from January 13 to 16, 2018. This year’s program theme is “Building an Embryo:
A Fertile Journey.” We developed this theme to integrate applied research that focuses on advancing and
improving male and female fertility in global livestock species with basic investigations that seek to iden-
tify and understand the molecular pathways and determinants of gamete acquisition of fertilizing and early
developmental potential and complex embryonic tissue differentiation and conceptus development. To this
end, we have secured the participation of leading researchers in these areas who have travelled from and
across several continents to share their knowledge and expertise with our society.
The 44th Annual Conference of the IETS is organized with five plenary sessions featuring 10 invited speak-
ers, who will provide in-depth overviews of various topics central to the main theme, and supplemented
with complementary short oral presentations selected from the submitted abstracts. This year, the major
topics include cloning and epigenetic regulation of gamete and embryo development and differentiation, in
vivo and in vitro strategies to enhance fertility and reduce generation intervals, and the contrasting fates of
mitochondria following fertilization in mammals. In this vein, Professor Justin St. John, Monash University,
Australia, will give the keynote lecture presentation titled “The mitochondrial genome: How it drives fertil-
ity.” In addition, poster sessions showcase the dynamics of our advances in animal biotechnology.
We are grateful to several people for their valuable contributions of time and effort in organizing this sci-
entific meeting. In particular, we acknowledge the supreme efforts of our IETS president, Professor Fulvio
Gandolfi, University of Milan, for selecting the conference location and arranging the venue, the closing
party, and the social and domestic activities. We also thank Dr. Richard Fry for organizing the Practitioners’
Forum, titled “Current and future status of reproductive technologies,” Dr. Jorge Piedrahita for organizing
the DABE Symposium, and Drs. Gabriela Mastromonaco and Pierre Comizzoli for organizing the precon-
ference CANDES Forum, titled “Monitoring and controlling ovarian activity in CANDES.” We thank the
main session speakers and their co-authors, the authors of the abstracts, and the participants in the student
competition for providing excellent scientific material. This year, 220 abstracts were submitted and 207
were accepted for publication in the conference proceedings. We are most grateful for the often-immediate
assistance of the manuscript reviewers, and we are particularly thankful to all the section editors and abstract
reviewers for their time and effort during the summer, when many were officially on vacation. We also thank
Dr. Charles Rosenkrans for organizing the student competition and for selecting the student finalists. We
would like to take this opportunity to mention Morulas and to thank them for developing an exciting pro-
gram of activities for all students to attend and for their help with day-to-day duties during the conference.
We also thank our session chairs and co-chairs.
We thank the IETS Board of Governors and Executive Board for their assistance in preparing for the 2018
Annual Conference. In particular, we are grateful for the sponsorship that they have brought on board and
extend our deepest gratitude to the sponsors for their economic contributions that make IETS 2018 possible.
We are indebted to Ms. Debi Seymour, the executive secretary of IETS, for her constant and immediate
support of our efforts during the past year and to Dr. Graeme Martin, editor-in-chief, and Jenny Foster, pub-
lisher, of Reproduction, Fertility and Development for their help in the production of the conference booklets
and proceedings.
Finally, we thank you all for attending and contributing to the conference. We hope that you have an excep-
tionally memorable experience at the 44th Annual Conference of the IETS, Bangkok.
Looking forward to seeing you all in Bangkok!
Trudee Fair and Daniel Salamone
2018 IETS Program Co-Chairs
International Embryo Technology Society 1

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2 44th Annual Conference

2018 Recipients of the IETS Pioneer Award
Marc-André Sirard David Thomas Armstrong
The Pioneer Award is bestowed by the International Embryo Technology Society (IETS) to
recognize individuals who have made seminal contributions to the development of embryo-
based technologies.
Award Presentation: Tuesday, January 16, at 14:15
Previous Recipients
H. Niemann (2017) S. Willadsen (2005) R. L. Brinster (1992)
C. E. Pope (2016) B. Brackett (2004) A. K. Tarkowski (1991)
K. H. S. Campbell (2015) K. Betteridge (2003) J. D. Biggers (1990)
J.-P. Renard (2015) R. H. Foote (2002) C. Thibault (1989)
W. W. Thatcher (2014) P. J. Dziuk (2001) A. L. McLaren and D. Michie
J. Hahn (2013) R. Yanagimachi (2000) (1988)
O. J. Ginther (2012) R. M. Moor (1999) E. J. C. Polge (1987)
I. Wilmut (2011) I. Gordon (1998) T. M. Sugie (1986)
R. J. Mapletoft (2010) S. Wintenberger-Torres (1997) L. E. A. Rowson (1985)
S. P. Leibo (2009) W. K. Whitten (1996) L. E. Casida (1984)
G. Seidel Jr. (2008) C. R. Austin (1995) M. C. Chang (1983)
A. Iritani (2007) N. W. Moore (1994) R. O. Berry (1982)
D. Kraemer (2006) R. G. Edwards (1993)

Map of the Venue
Shangri-La Hotel
89 Soi Wat Suan Plu Khwaeng Bang Rak,
Khet Bang Rak, Krung Thep Maha Nakhon 10500, Thailand
Shangri-La Wing (Lobby Level)
Shangri-La Wing (Level 1)

Map of the Venue
Shangri-La Hotel
89 Soi Wat Suan Plu Khwaeng Bang Rak,
Khet Bang Rak, Krung Thep Maha Nakhon 10500, Thailand
Krungthep Wing (Level 3)

General Information
Meeting Room Directory

Main conference sessions Ballroom II and III; concurrent sessions: Myanmar, Practitioners’ Forum
Exhibits Ballroom I and Pre-function Foyer
Poster displays Ballroom I and Pre-function Foyer
Please see the Scientific Program for additional room assignments.
Registration Desk Hours

The registration desk is located outside Ballroom III.
Pick up of preregistration packets

Friday, January 12 16:00–19:00 (Board room)
On-site registration hours

Saturday, January 13 07:00–18:00
Sunday, January 14 07:00–18:00
Monday, January 15 07:30–16:00
Tuesday, January 16 08:00–15:00
Exhibit Information
Ballroom I
Setup
Saturday, January 13 09:00–17:00
Exhibits open
Sunday, January 14 09:00–19:00
18:00–19:00 (Reception)
Monday, January 15 09:00–17:00
Teardown
Details on the exhibitors can be found in the Exhibit Directory on page 49.
Badges
As a security requirement, we request that all participants wear their conference name badges to all sessions and
social functions.
Certificates of Attendance and Presentation

A Certificate of Attendance will be included in your badge packet.

Currency
The official currency of Thailand is the Thai baht (pronounced baaht). One baht is divided into 100 satang.
Coins come in denominations of 1, 2, 5, and 10 baht as well as 25 and 50 satang. (You may get some 25 or 50
satang coins in change at a supermarket.) Banknotes come in denominations of 20, 50, 100, 500, and 1,000 baht.
The most commonly used coin is the 10 baht, and the most commonly used note is the 100 baht. The notes of 20
and 50 baht have changed, with a slightly different portrait of the king.
• Travellers’ cheques are generally accepted only at dedicated foreign exchange shops or banks.
• Automated teller machines (ATMs) are plentiful throughout Thailand, and most will accept cards issued by
any of the major international banking networks (Plus, Cirrus, etc.).
Foreign debit and credit card withdrawals from Thai ATMs incur a 150 or 180 baht fee levied by the local ATM
owner in addition to any fees added by your home financial institution.
• Major credit cards such as Visa, MasterCard, JCB, and American Express are readily accepted at most hotels,
airlines, restaurants, and upscale merchants.
• To prevent your credit or debit card from being declined, it is important to advise your card issuer of your
travel plans in advance.
• Some institutions routinely block or deny unexpected charges from Thai merchants for fear of possible fraud-
ulent use.
Buying Thai Baht Outside Thailand

Thai baht is widely stocked by most currency suppliers outside Thailand. However, rates obtained in Thailand
are higher and money can be exchanged on arrival at more advantageous rates.
Exchanging Cash in Thailand is Easy

FOREX (FOreign EXchange) booths are very common and clearly post their daily exchange rates on an elec-
tronic notice board.
• Cash exchanges in Thailand incur NO commissions or fees.
• Do not buy any Thai currency until you arrive in Thailand. The rates offered in Thailand are ALWAYS
BETTER than you could get back home (for "mainstream" currencies).
• FOREX booths accept ALL MAJOR currencies.
• DO remember to bring ONLY bank notes in good overall condition. FOREX booths generally WILL NOT
accept bank notes that are torn, ripped, damaged, or excessively marked or stamped.
Climate
In January, the sun shines for at least 9 hours per day, with on average 2 days of rainfall during the month.
Around 10 mm of rain falls throughout January, with temperatures generally settling between 21°C (69°F) and
32°C (90°F).
Electricity
In Thailand the standard voltage is 220 V and the frequency is 50 Hz. You can use your electric appliances in
Thailand if the standard voltage in your country is between 220 and 240 V (as in the United Kingdom, Europe,
Australia, and most of Asia and Africa). Manufacturers take these small deviations into account. If the stan-
dard voltage in your country is in the range of 100 to 127 V (as in the United States, Canada, and most South
American countries), you will need a voltage converter in Thailand.
Registration Fees
All registration fees will be paid in US dollars or credit card purchases.
Messages
Any messages received for conference delegates will be posted on the message board located near the registration
desk.
Refreshments
Morning and afternoon refreshments and lunch are included in your registration fee this year and are provided
during the scheduled break times in the exhibit area located in Ballroom I.

Dining and Entertainment
The restaurants at Shangri-La Hotel, Bangkok, offer a range of international culinary choices. Enjoy traditional
Thai or Asian and cosmopolitan dishes or be tempted by an international buffet on board Horizon Cruise, their
private river cruise ship. Dine at Volti Ristorante and Bar for authentic Italian dishes or at Shang Palace for
Chinese favorites.
Sip on cocktails at NEXT2 Upper Deck, or enjoy light snacks and afternoon tea at the various lounges within the
hotel’s premises.
Services and Amenities

A riverside swimming pool, tennis courts, and a well-equipped fitness center with a range of classes are
available.
The award-winning CHI, The Spa at Shangri-La, offers some of the largest private spa suites in Bangkok. The
spa has a diverse menu of treatments drawn from the ancient healing traditions of Asia.
The conference hotel room rate includes a breakfast buffet and WiFi internet access in the hotel room and
selected public areas of the hotel.
DELIVERING THE BEST

IVF SOLUTIONS

Program
Thursday, January 11
09:00–17:00 IETS Board of Governors meeting (The Chairman’s Room)
Friday, January 12
09:00–17:00 IETS Board of Governors meeting (The Chairman’s Room)
09:00–13:00 HASAC Research Subcommittee meeting (Valley I)
14:00–18:00 HASAC Regulatory Subcommittee meeting (Valley I)
16:00–19:00 Registration (Board Room)
Saturday, January 13
07:00–18:00 Registration (Ballroom Foyer)
08:30–15:45 CANDES Preconference Symposium (Ballroom II)
Monitoring and Controlling Ovarian Activity in CANDES
12:00–17:00 Commercial exhibit setup and poster setup (Ballroom I and Foyer)
16:00–19:00 Morulas Workshop (The Study)
Assessment of Mammalian Embryo Quality: Invasive and Non-Invasive Techniques
Featured speakers: George Seidel, Colorado State University, USA; Heiner Niemann, Friedrich-
Loeffler Institute, Germany; and Jeremy Thompson, University of Adelaide, Australia
16:00–20:00 IETS Foundation Board of Trustees meeting (Garden Gallery)
Sunday, January 14
07:00–08:30 Poster setup (Ballroom I and Foyer)
07:00–08:30 Past Presidents’ breakfast (Valley II)
07:00–08:30 Graduate and Undergraduate Student Competition Presenters’ breakfast with IETS Foundation
education chair (Valley I)
09:00–19:00 Commercial exhibits (Ballroom I and Foyer)
08:30–08:45 Opening and welcome: Trudee Fair and Daniel Salamone (Ballrooms II and III)
Session I: Clones and Lives Less Ordinary (Ballrooms II and III)

Session co-chairs: Cesare Galli, AVANTEA, Italy, and Pasqualino Loi, University of Teramo, Italy
08:45–09:30 The promise of dog cloning
Byeong Chun Lee, Seoul National University, South Korea
09:30–10:15 A journey through horse cloning
Andres Gambini, Universidad de Buenos Aires, Argentina
10:15–10:30 Recent advances in cloning by somatic cell nuclear transfer in camelids
N. A. Wani, V. S. Binoy, and S.-B. Hong (Abstract 34)
10:30–11:00 Refreshment break/poster viewing and exhibits
IETS Foundation Student Competition Presentations (Ballrooms II and III)

Session chair: Charles F. Rosenkrans, University of Arkansas
11:00–11:15 Microinjection of CPE-binding protein polyadenylated mRNA increases developmental
competence of bovine oocytes in vitro
M. Yang, Z. Fan, and I. A. Polejaeva (Abstract 1)
11:15–11:30 Response to treatment with human chorionic gonadotropin on pregnancy rate of heat-stressed
lactating cows: Interactions with parity and genotype
A. M. Zolini, W. Ortiz, E. Eliab, D. Serdal, and P. J. Hansen (Abstract 2)
11:30–11:45 Animal protein-free semen extender for fixed-time insemination of beef cows
S. X. Yang, E. M. Zwiefelhofer, G. P. Adams, and M. Anzar (Abstract 3)
11:45–12:00 Relationship between ovarian vascularity, cumulus-oocyte morphology, and luteal development
in four-month-old calves after FSH stimulation
S. L. Caunce, A. R. T. Krause, F. C. F. Dias, G. P. Adams, and J. Singh (Abstract 4)
12:00–12:15 Live offspring produced from reproductive material recovered during the annual cull of bison
from Yellowstone National Park
H. Benham, M. McCollum, P. Nol, B. Frey, J. Rhyan, and J. Barfield (Abstract 5)
12:15–12:30 CRISPR-generated ZP4 knockout rabbits exhibit impaired in vivo pre-implantation development
I. Lamas-Toranzo, N. F. Balvis, P. L. Lorenzo, P. G. Rebollar, M. Avilés, and P. Bermejo-Álvarez
(Abstract 6)
12:30–14:00 Lunch break (located in Ballroom I and Foyer)
12:30–14:00 IETS Board luncheon with affiliate society representatives (Garden Gallery)
12:30–14:00 HASAC Manual and Certificates Subcommittee meeting (Valley I)
12:30–14:00 Morulas and Mentor luncheon (Valley II)
Session II: Ever-Decreasing Generation Intervals (Ballrooms II and III)

Session co-chairs: Dimitrios Rizos, National Institute for Agriculture and Food Research and Technology, Spain,
and Beatriz Fernandez-Fuertes, University College Dublin, Ireland
14:00–14:45 Preantral follicle culture and oocyte quality
Ursula Eichenlaub-Ritter, Bielefeld University, Germany
14:45–15:30 Recent advances for spermatogonial stem cell transplantation in livestock
Jon M. Oatley, Washington State University, USA
15:30–16:00 Refreshment break/poster viewing and exhibits (Ballroom I and Foyer)
Selected Short Presentations (Ballrooms II and III)

16:00–16:15 In vitro generation and characterization of putative primordial germ cells derived from induced
pluripotent stem cells in cattle
F. F. Bressan, M. A. Lima, L. S. Machado, N. C. G. Pieiri, P. Fantinato-Neto, J.
Therrien, F. Perecin, L.. C. Smith, and F. V. Meirelles (Abstract 183)
16:15–16:30 Culture conditions supporting long-term expansion of bovine spermatogonial stem cells isolated
from adult and immature testes
Suyatno, Y. Kitamura, N. Minami, M. Yamada, and H. Imai (Abstract 192)
16:30–16:45 Directed differentiation of porcine induced pluripotent stem cells into all three germ layers via
embryoid body formation
W. Chakritbudsabong, S. Pamonsupornvichit, L. Sariya, R. Pronarkngver, S.
Chaiwattanarungruengpaisan, S. Klinsrithong, J. N. Ferreira, and S. Rungarunlert (Abstract
185)
16:45–17:15 Distinguished Service Award (Ballrooms II and III)
18:00–19:00 Welcome reception (Ballroom I and Foyer)
Monday, January 15

Session III: Maternal–Paternal Interactions (Ballrooms II and III)
Session co-chairs: Pat Lonergan, University College Dublin, Ireland, and Adriana Zolini, University of Florida,
USA
08:00–08:45 Paternal priming of maternal tissues to optimize pregnancy success
John J. Bromfield, University of Florida, USA
08:45–09:30 Post-fertilization sperm mitophagy: The tale of mitochondrial Eve and Steve
Peter Sutovsky, University of Missouri, USA

Session co-chairs: Rebecca Krisher, National Foundation for Fertility Research, and Kathryn Polkoff, University
of Illinois
09:30–09:45 Single-cell RNA sequencing reveals blastomere heterogeneity and early lineage specification
events in bovine embryos during major embryonic genome activation
I. Lavagi, S. Krebs, K. Simmet, V. Zakhartchenko, E. Wolf, and H. Blum (Abstract 108)
09:45–10:00 Compensation of the growth and development of individually transferred bovine bisected
embryos
E. I. Schild, A. E. Ynsaurralde-Rivolta, S. Lopez-Valiente, S. Maresca, C. Munar, A. M.
Rodriguez, S. Munilla, D. Hernandez Maizon, N. Bosetti, M. Curti, J. I. Jaca, R. Bevacqua, A.
Rogberg-Munoz, and D. F. Salamone (Abstract 82)
10:00–12:00 Poster session I (Ballroom I and Foyer)
All odd-numbered posters except those in the Student Competition and the Undergraduate
Poster Competition need to be removed by 14:00 today. Even-numbered posters will be
exhibited from Monday at 14:00 to Tuesday at 14:00
10:00–12:00 Exhibits (Ballroom I and Foyer)
12:00–13:30 IETS Data Retrieval Committee meeting (Valley I)
12:00–13:30 IETS Exhibitors’ luncheon with IETS Board of Governors (Garden Gallery)
12:00–13:30 Morulas career luncheon (Valley II)
Session IV: The Epigenetics of Building an Embryo and Stem Cell (Ballrooms II and III)
Session co-chairs: Daniel F. Salamone, Universidad de Buenos Aires, and Hayley Benham, Colorado State
University, USA
13:30–14:15 Methylation mechanisms and biomechanical effectors controlling cell fate
Tiziana A. L. Brevini, Università degli Studi di Milano, Italy
14:15–15:00 Transcriptional and epigenetic control of cell fate decisions in early embryo
Ramiro Alberio, University of Nottingham, United Kingdom
15:00–15:30 Peter Farin Trainee Award Winners Presentations (Ballrooms II and III)
15:30–16:00 Refreshment break/poster viewing and exhibits (Ballroom I and Foyer)
Concurrent Forum
16:00–18:00 Practitioners’ Forum (Myanmar)
Chair: Richard Fry
Current and Future Status of Reproductive Technologies
Topics: AI—
MOET—Rainer Saner, Swissgenetics
IVF—John Hepburn, Animal Breeding Services Ltd.
Cloning—Andres Gambini, Universidad de Buenos Aires

Concurrent Forum
16:00–18:00 Domestic Animal Biomedical Embryology Committee (DABE) (Ballrooms II and III)
Chair: Jorge Piedrahita, North Carolina State University
16:00–16:10 Welcome and introduction: Jorge Piedrahita, DABE chair
16:10–17:00 Imaging the molecular and cell dynamics that form the early mouse embryo
Nicolas Plachta, Institute of Molecular and Cell Biology, A*STAR, Singapore
Our goal is to reveal how mammalian cells resolve their fate, shape, and position in the body
in real time. Understanding how these decisions are made is critical to realise how embryos
form and what problems compromise human fertility, yet their real-time control in vivo remains
unknown. Because fixed specimens cannot capture cell dynamics, we established imaging
technologies to study cells directly in live mouse embryos. We recently showed how transcription
factors search and bind to the DNA to determine the first cell fates of the embryo. We found
that differences in the binding of the transcription factor Sox2 to DNA appear as early as the
four-cell stage of development and predict cell fate. We also discovered that as cells choose their
initial fates, they extend long filopodia protrusions to pull their neighbour cells closer, revealing
a mechanism for embryo compaction and polarization. Finally, we established techniques to
image how dynamic changes in the organisation of the actin and microtubule cytoskeletons
regulate the first spatial segregation of inner and outer cells to form the future pluripotent inner
mass and placental tissues of the embryo. Together, our findings reveal some of the key dynamic
mechanisms that pattern the early mouse embryo.

17:00–17:15 Rescue of the hematoendothelial phenotypes in the ETV2-null cloned pig via embryo
complementation
G. Maeng, X. Pan, S. Das, K.-D. Choi, N. Koyano-Nakagawa, M. G. Garry, and D. J. Garry
(Abstract 25)
17:15–17:30 Generation of immunoglobulin heavy constant Mu (IGHM) knockout goats using CRISPR/Cas9
and somatic cell nuclear transfer
Z. Fan, M. Regouski, A. J. Van Wettere, Z. Wang, E. Sullivan, and I. A. Polejaeva (Abstract 28)
17:30–17:45 Profile of microRNAs of equine amniotic mesenchymal cell-derived microvesicles in different
time-span of in vitro culture
A. Lange-Consiglio, E. Capra, F. Pizzi, B. Lazzari, C. Perrini, A. Stella, and F. Cremonesi
(Abstract 190)
17:45–18:00 Optimization of transfection efficiency for CRISPR/Cas9-induced genomic editing in porcine
fibroblast cells
S. N. Lanjewar and K. R. Bondioli (Abstract 205)
18:00–18:30 IETS Business Meeting (Ballrooms II and III)
18:30–19:30 HASAC open meeting (Ballrooms II and III)
18:30–19:30 Morulas forum (The Study)
20:00–21:00 Morulas student mixer (TBD)
Tuesday, January 16
07:30–08:30 Organizational meeting of the IETS Board of Governors (The Chairman’s Room)
Session V: Survival of the Fittest Embryo (Ballrooms II and III)

Session co-chairs: Marcelo Seneda, University of Londrina, Brazil, and Camille C. Goblet, Texas A&M
University, USA
08:30–09:15 Fertility and the transition dairy cow
John Roche, Ag Research, New Zealand
09:15–10:00 Early onset of puberty in cattle: Implications for gamete quality and embryo survival
David Kenny, Teagasc, Ireland

10:00–10:15 Distribution of gonadotropin-releasing hormone and kisspeptin neurons in the preoptic area and
hypothalamus during the estrous cycle in cows
C. E. P. Leonardi, R. Carrasco, F. F. C. Dias, G. P. Adams, and J. Singh (Abstract 104)
10:15–10:30 Selected reaction monitoring-based absolute quantification of developmentally relevant proteins
in early bovine embryos reveals differences between in vitro and in vivo embryo culture and
between different maternal metabolic stages
G. J. Arnold, K. Gegenfurtner, T. Frohlich, D. R. Deutsch, P. Salvetti, N. Forde, P. Lonergan, U.
Besenfelder, and E. Wolf (Abstract 59)
10:30–12:30 Poster session II (Ballroom I and Foyer)
Even-numbered posters, along with the Student Competition and the Undergraduate Poster
Competition, will be exhibited from Monday at 14:00 to Tuesday at 14:00
*All posters must be removed before 14:00 on Tuesday, January 16.
12:30–14:00 Organizational lunch meeting of the IETS Foundation (Valley I)
12:30–14:00 2018, 2019, and 2020, IETS Program Committee lunch (Valley II)
14:00–17:00 Commercial exhibit takedown (Ballroom I and Foyer)
14:15–15:30 Pioneer Award (Ballrooms II and III)
Session VI: Keynote Lecture (Ballrooms II and III)

Session chair: Fulvio Gandolfi, Università Degli Studi Di Milano, Italy
15:30–16:15 The mitochondrial genome: How it drives fertility
Justin St. John, Monash University, Australia
Awards Presentations and Updates (Ballrooms II and III)

16:15–16:45 IETS Foundation Early Career Achievement Award winner
16:45–17:30 IETS Foundation Student Competition Awards; CANDES, DABE, and HASAC updates
17:30–17:45 Closing ceremony
20:45–23:45 Closing party, Grand Chaopraya cruise

www.publish.csiro.au/rd

The Program Co-Chairs Acknowledge and Thank the Following People
Section Editors
Charles Rosenkrans, Student Competition Christine Wrenzycki, Gene Expression
Joao Bastista Borges, Artificial Insemination Hiroaki Funahashi, IVF/IVP
Daniel Salamone, Cloning/Nuclear Transfer John Kastelic, Male Physiology
Pierre Comizzoli, Cryopreservation/Cryobiology Trudee Fair, Oocyte Activation
Pete Hansen, Developmental Biology Rebecca Krisher, Oocyte Maturation
Pat Lonergan, Early Pregnancy/Pregnancy Daniel Salamone, Sexing
Recognition Katrin Hinrichs, Sperm Injection
Alfonso Gutiérrez-Adán, Embryo Culture Tiziana Brevini, Stem Cells
Marcelo Bertolini, Embryo Manipulation Osamu Dochi, Superovulation
Jeremy Block, Embryo Transfer Carol Keefer, Transgenesis
Ann Van Soom, Epidemiology/Diseases Charles Rosenkrans, Undergraduate Poster
Barbara Durrant, Exotic Species Competition
Fulvio Gandolfi, Folliculogenesis/Oogenesis
Manuscript and Abstract Reviewers

Mohan Acharya Natalie Calatayud Alexsandra Fernandes Wilfredo Huanca
Tomas J. Acosta Henrik Callesen Pereira Kei Imai
Gregg Adams Lino Fernando Beatriz Fernandez-Fuertes Thomas Jensen
Tom A. Aire Campos-Chillon Muriel Filliers S. W. Jiang
Paulo L. Aguiar Natalia Canel L. Fraser John Kastelic
Ramiro Albeiro Elaine Carnevale Hiroaki Funahashi Carol Keefer
Twink Allen Peter Chenoweth Joaquin Gadea M. S. Kheirkhah
Gary Althouse Younes Chorfi Bart Gadella K. Kikuchi
Gary B. Anderson Philippe Collas Cesare Galli Ill Hwa Kim
A. R. Bahrami Pierre Comizzoli Andres Gambini Koji Kimura
Barry Ball Donald F. Conrad Fulvio Gandolfi Rob Knox
Jennifer Barfield Belen Crespo Bianca Gasparrini Rebecca Krisher
Pietro Baruselli Andre Dalto John Gibbons Wilfried Kues
Guilherme Bastos Amanda Davis Karim Golshahi F. Kutluyer
Carlos Batista Duane Davis Karen Goodrowe M. A. Lagares
Don Bergfelt Catharina de Schauwer Martha Gomez Irina Lagutina
Pablo Bermejo Brad Didion François-Xavier Grand Anna Lange Consiglio
Mari Bernardi Margot Dode Hanna Grothmann Giovanna Lazzari
Marcelo Bertolini Veronique Duranthon Bernard Guerin Sergio Ledda
Romina Bevacqua Barbara Durrant M. Gumulka H. J. Liu
Mario Binelli Judith Eckert Alfonso Gutiérrez-Adán Deirdre Logsdon
Gabriel Bo Trudee Fair Frank Gwazdauskas Charles Long
Heiner Bollwein Laura Falchi Peter Hansen Pat Lonergan
Ken Bondioli Charlotte E. Farin Hiroshi Harayama Julie Long
Vilceu Bordignon Richard Fayrer-Hosken Yutaka Hashiyada Charles Looney
Fernando Bortolozzo Alireza Fazeli John Hasler Barbara Loureiro
Tiziana Brevini Carleigh Fedorka Jason Herrick Matt Lucy
Magdalena Bryla Ricardo Delner M. Hidalgo Andrea Lucas-Hahn
Carla Buemo Cristiano Feltrin Katrin Hinrichs David MacHugh
Jose Buratini Ines Hiriart Ken MacMillan

Reuben Mapletoft Jennifer Patterson Timothy Safranski Robin Tervit
Colazo Marcos J. Peng Daniel Salamone William Thatcher
A. Maria Georgia Pennarossa Haney Samir Paula Tribulo
Rafael Fernandez Martan Parker Pennington E. G. Sanches Jeremy Thompson
Marc Maserati Federico Pereyra-Bonnet Marina Sansinena Joao H. M. Viana
Gabriela Mastomonaco Eduardo Perez Nicolas Santiquet Ann Van Soom
Satoko Matoba Serafin Perez J. E. Santos Gabriel Vichera
Flavio V. Meirelles George Perry Roberto Sartori A. T. M. Viveiros
Pascal Mermillod Peter Pfeffer Virginia Savy Eric Walters
Marcella Milazzotto Jorge Piedrahita Andrew Schiffmacher Gen Watanabe
Marcelo Miragaya Irina Polejaeva George Seidel David Wells
Lucia Moro Earle Pope Marcelo Marcondes Kevin Wells
Lara Mouttham Randy Prather Seneda Matthew Wheeler
Marta Munoz Christopher Price Hosup Shim Ken White
Clifton Murphy Guilherme Pugliese Jaswant Singh Milo Wiltbank
Masashi Nagano Detlef Rath Marc-André Sirard Eckhard Wolf
Subir K. Nagdas Bethany Redel Elena Silva Christine Wrenzycki
Lucky Nedambale Eduardo Ribeiro Tamas Somfai Jimena Yapura
Veronica Negron Perez Dimitrios Rizos Joanna Souza-Fabjan Naoto Yonezawa
Heiner Niemann Rebecca Robker Lee Spate Shosei Yoshida
Monika Nowak Ileretny Ed Squires Curt Youngs
Jon Oatley Rodriguez-Alvarez Lawrence Smith Ye Yuan
Bjorn Oback Josã Luiz Rodrigues Katrien Smits Rogerio Zacariotti
Sofia Ortega Maria Belen Rodriguez Roger Sturmey Alessandro Zenobi
Lynne O-Shea Rick Rorie Peter Sutovsky Leticia Zoccolaro
John Parrish Charles Rosenkrans K. Taya Adriana Zolini
Rolando Pasquariello Pablo Ross Bhanu Telugu

Poster Session Information
Location
Posters are located in Ballroom I and the Foyer of the Shangri-La Hotel on the lobby level (see map on
page 4).
Poster Numbers
Posters are identified by the number corresponding to the abstract number in Reproduction, Fertility and
Development 2018; 30 (1). Numbering of the posters begins at 1 and ends at 208.
Setup
Odd-numbered posters can be put up from 12:00 to 17:00 on Saturday, January 13, 2018, and from 06:30 to
08:00 on Sunday, January 14, 2018. They will remain up until 14:00 on Monday, January 15. All odd-numbered
posters must be removed by 14:00, Monday, January 15.
Even-numbered posters can be put up on Monday at 14:00. They will remain up until 13:00 on Tuesday, January
16.
Poster Session I
Presentations by authors of odd-numbered abstracts (e.g., 7, 9, 11) in Reproduction, Fertility and Development
2018; 30 (1) as well as the Student Competition finalist and Undergraduate finalist poster presentations will take
place Monday, January 15, 2018, from 10:00 to 12:00. Odd-numbered posters for the poster competition will also
be judged on Monday, January 15, from 10:00 to 12:00.
Poster Session II
Presentations by authors of even-numbered abstracts (e.g., 8, 10, 12) in Reproduction, Fertility and Development
2018; 30 (1) will take place Tuesday, January 16, 2018, from 10:30 to 12:30. Even-numbered posters for the
poster competition will be judged on Tuesday, January 16, from 10:30 to 12:30.
Teardown
Poster teardown for the even-numbered posters will take place from 12:30 to 14:00 Tuesday, January 16, 2018.
Posters that are not taken down by 14:00 on Tuesday will be taken down and discarded.

Poster Session Order by Topic
Poster number = abstract number in Reproduction, Fertility and Development 2018; 30 (1)
Student Competition
1 Microinjection of CPE-binding protein polyadenylated mRNA increases developmental
competence of bovine oocytes in vitro
M. Yang, Z. Fan, and I. A. Polejaeva
2 Response to treatment with human chorionic gonadotropin on pregnancy rate of heat-stressed
lactating cows: Interactions with parity and genotype
A. M. Zolini, W. Ortiz, E. Eliab, D. Serdal, and P. J. Hansen
3 Animal protein–free semen extender for fixed-time insemination of beef cows
S. X. Yang, E. M. Zwiefelhofer, G. P. Adams, and M. Anzar
4 Relationship between ovarian vascularity, cumulus-oocyte morphology and luteal development in
four-month-old calves after FSH stimulation
S. L. Caunce, A. R. T. Krause, F. C. F. Dias, G. P. Adams, and J. Singh
5 Live offspring produced from reproductive material recovered during the annual cull of bison
from Yellowstone National Park
H. Benham, M. McCollum, P. Nol, B. Frey, J. Rhyan, and J. Barfield
6 CRISPR-generated ZP4 knockout rabbits exhibit impaired in vivo pre-implantation development
I. Lamas-Toranzo, N. F. Balvís, P. L. Lorenzo, P. G. Rebollar, M. Avilés, and P. Bermejo-Álvarez
Artificial Insemination
7 Ovarian follicular dynamics and conception rate in Bos indicus cows with different antral follicle
counts subjected to timed artificial insemination
F. Morotti, R. Moretti, G. M. Gomes dos Santos, K. C. Silva-Santos, and M. M. Seneda
8 Effect of single layer centrifugation with Percoll Plus® of fresh bull semen on plasma membrane
stability and lipid peroxidation after cryopreservation
T. E. Cruz, A. Martins Jr., F. N. Marqui, T. I. U. Berton, C. P. Freitas-Dell’Aqua, D. G. Souza,
and S. H. V. Perri
9 Single layer centrifugation of bull semen through Percoll Plus® before cryopreservation
A. Martins Jr., F. N. Marqui, T. E. Cruz, T. I. H. Berton, D. G. Souza, and S. H. V. Perri
10 Evaluation of frozen sperm quality after single layer centrifugation with Percoll Plus® of fresh
bull semen
F. N. Marqui, A. Martins Jr., T. E. Cruz, T. I. U. Berton, C. P. Freitas-Dell’Aqua, D. G. Souza,
and S. H. V. Perri
11 Early induction of luteolysis in fixed-time artificial insemination protocols increases fertility in
beef cows
J. B. Borges, A. B. Machado, E. Pradebon, M. Dias, M. Bernardi, and M. Wiltbank
12 Ovulation timing following an aromatase inhibitor-based synchronization protocol in beef heifers
and cows
E. M. Zwiefelhofer, W. Lillico, and G. P. Adams
13 Pre-synchronization by induction of a dominant follicle using a progesterone device in a
GnRH-based-ovulation synchronization protocol in lactating dairy cows
M. P. Bottino, L. A. C. L. DaSilva, L. M. S. Simoes, G. Santos, I. Y. H. Martinez, P. A. M. Barros,
J. C. DeSouza, P. S. Baruzelli, and J. N. S. Sales

14 Composition of semen extenders and varying seminal plasma concentrations affects formation of
neutrophil extracellular traps in the bovine system
T. Fichtner, F. Kotarski, L. Silva, C. Hermosilla, A. Taubert, and C. Wrenzycki
15 Effects of timed artificial insemination protocol using prostglandin F2α and estradiol benzoate on
conception rate in high-producing dairy cows
M. Yamaguchi, M. Takayama, and H. López
16 Evaluation of factors influencing timed artificial insemination in South African communal cows
Z. C. Raphalalani, T. L. Nedambale, M. L. Mphaphathi, M. M. Seshoka, M. Nkadimeng, M. A.
Bopape, F. L. Seolwana, M. H. Mapeka, F. V. Ramukhithi, and K. A. Nephawe
17 Relationship between semen parameters and morphometric characteristics of Zulu rams
J. N. Ngcobo, A. Maqhashu, F. V. Ramukhithi, L. P. Kruger, K. A. Nephawe, and T. L. Nedambale
18 Wireless cloud-based data acquisition and management tool for use with Inteli-Straws and
assisted reproductive techniques
M. E. Kjelland, S. Romo, and T. K. Stroud
19 An efficient noninvasive alternative recipient preparation in swamp buffalo using the Ovsynch
protocol
T. Chaikhun-Marcou, W. Maitreejet, S. Majarune, K. Karnjanasirm, S. Rattanatabtimtong, and T.
Raungprim
20 Assessment of motion and kinematic characteristics of semen from four cattle breeds using
computer-aided sperm analysis
M. L. Mphaphathi, M. M. Seshoka, T. R. Netshirovha, Z. C. Raphalalani, N. Bovula, M. R.
Thandavhathu, B. Raito, N. L. Kanuya, A. Maqhashu, and T. L. Nedambale
21 Comparative study on epididymal spermatozoa traits of Large White × Landrace and Kolbroek
boars
T. R. Netshirovha, A. T. Kanengoni, M. B. Matabane, M. L. Mphaphathi, A. Maqhashu, N.
Bovula, M. Nkadimeng, and M. Chimonyo
22 Comparing three extenders: Hashi, Green Buffer and INRA 96, for chilled storage of Bactrian
camel semen
F. Panahi, A. Niasari-Naslaji, F. Seyedasgari, T. Ararooti, H. Adel, and A. Kalantari
Cloning/Nuclear Transfer
23 Production of genetically modified founder pigs as models for human diseases
H. Callesen, Y. Liu, H. S. Pedersen, C. B. Sørensen, and J. F. Bentzon
24 Evaluation of latrunculin A for the activation of hand-made cloning (HMC) porcine embryos
F. K. Castañeda, N. G. Canel, G. V. Landschoot, A. De Stéfano, R. J. Bevacqua, and D. F.
Salamone
25 Rescue of the hematoendothelial phenotypes in the ETV2-null cloned pig via embryo
complementation
G. Maeng, X. Pan, S. Das, K.-D. Choi, N. Koyano-Nakagawa, M. G. Garry, and D. J. Garry
26 Drugs that modify epigenetics…What do they do to porcine clones?
C. P. Buemo, A. Gambini, L. N. Moro, N. Canel, and D. F. Salamone
27 Quisinostat, a potent histone deacetylase inhibitor, regulates the expression of pluripotency- and
reprogramming-related genes on somatic cell nuclear transferred porcine embryos
A. Taweechaipaisankul, J.-X. Jin, S. Lee, G. A. Kim, and B. C. Lee
28 Generation of immunoglobulin heavy constant mu (IGHM) knockout goats using CRISPR/Cas9
and somatic cell nuclear transfer
Z. Fan, M. Regouski, A. J. Van Wettere, Z. Wang, E. Sullivan, and I. A. Polejaeva

29 Co-incubation of equine cloned embryos with sialic acid: Effect on pregnancy rate
D. Vichera, R. Olivera, V. Arnold, J. Vergara, R. Jordan, and G. Vichera
30 Vitrification of equine embryos: Application in a commercial cloning program
G. Vichera, R. Jordan, V. Arnold, D. Dobler, and R. Olivera
31 Overall goat cloning efficiency under suboptimal conditions—A 6-year experience
L. H. Aguiar, C. E. Méndez-Calderón, F. L. Ongaratto, R. Rossetto, D. Rondina, J. L. Rodrigues,
L. R. Bertolin, and M. Bertolini
32 MicroRNA-29b improves the quality and developmental potential of blastocysts derived from
somatic cell nuclear transfer in cattle
W.-J. Zhou, S. Liang, and X.-S. Cui
33 Improved dog cloning efficiency using post-activation with Ro-3306, a Cdk1 inhibitor
M. J. Kim, H. J. Oh, E. M. N. Setyawan, S. H. Lee, and B. C. Lee
34 Recent advances in cloning by somatic cell nuclear transfer in camelids
N. A. Wani, V. S. Binoy, and S.-B. Hong
35 Interspecies somatic cell nuclear transfer embryos that form nucleoli do not always activate
mitochondrial functional differentiation at the time of embryonic genome activation
I. Lagutina, G. Lazzari, and C. Galli
36 Transgenic somatic cell nuclear transfer blastocyst selection with embryo biopsying
M. Nõmm, M. Ivask, P. Pärn, Ü. Jaakma, and S. Kõks
37 Healthy foals produced using bone marrow-mesenchymal stem cells as nuclear donors in horse
cloning
R. Olivera, L. Moro, R. Jordan, C. Luzzani, S. Miriuka, and G. Vichera
38 Improved cloning efficiency and developmental potential in bovine somatic cell nuclear transfer
with the new technology
L. Xu, M.-D. Joo, A. Mesalam, S.-H. Song, S. Zhang, and I.-K. Kong
39 Embryo development of Kazakh argali (Ovis ammon collium) by handmade cloning
Y. Toishibekov, E. Asanova, M. Yermekova, A. Seisenbayeva, D. Toishybek, and G. Vajta
Cryopreservation/Cryobiology
40 Gene expression profiling of in vitro-produced blastocysts derived from in vitro-matured bovine
oocytes vitrified/warmed in media supplemented with a biopolymer produced by an Antarctic
bacterium
N. Arcarons, M. Vendrell, M. Yeste, M. E. Mercadé, M. López-Béjar, and T. Mogas
41 Effects of dimethyl sulfoxide- or glycerol-based vitrification protocols on zona pellucida
hardening in mature bovine oocytes
K. D. Rogers, B. A. Foster, E. J. Guiterrez, F. A. Diaz, and K. R. Bondioli
42 Glycerol dilution in the straw by mixing methanol and sucrose for bovine embryo
cryopreservation
T. T. Takeda
43 Cryopreservation of bovine sperm using antifreeze polyamino-acid
T. Fujikawa, S. Imamura, M. Tokumaru, T. Ando, Y. Gen, S.-H. Hyon, and C. Kubota
44 Proliferation and antral formation of preantral follicle within cryopreserved cat ovarian tissue
transplanted into nude mice
N. Tanpradit and K. Chatdarong
45 DNA fragmentation of epididymal freeze-dried ram spermatozoa impairs embryo development
L. Palazzese, D. A. Anzalone, J. Gosálve?, P. Loi, and J. Saragusty
46 Effect of Bioxcell® and Triladyl® extenders on washed semen of South African indigenous bucks
L. P. Nethenzheni, M. L. Mphaphathi, N. C. Negota, and T. L. Nedambale
47 A new device and method for successful vitrification of in vitro-produced ovine embryos
S. Ledda, J. M. Kelly, S. K. Walker, Y. Natan, and A. Arav
48 Effect of dimethyl sulfoxide- or glycerol-based vitrification protocols on meiotic spindle of in
vitro-matured bovine oocytes
E. J. Gutierrez, F. A. Diaz, B. A. Foster, and K. R. Bondioli
49 Developmental potential of dromedary camel oocytes vitrified at the germinal vesicle stage:
Effects of different cryoprotectant combinations and cryo-carriers
M. Fathi, A. R. Moawad, and M. R. Badr
50 Effects of cathepsin B inhibitor E64 on the survival rate of cryopreserved semen from Korean
brindled bulls
S. W. Kim, M.-S. Kim, C.-L. Kim, and I. S. Jeon
51 Effect of cryoprotectant exposure time on development of vitrified-warmed immature equine
oocytes
H. S. Canesin, J. G. Brom-de-Luna, Y.-H. Choi, A. M. Pereira, G. G. Macedo, and K. Hinrichs
52 Difference of seminal plasma proteins in good- and poor-freezability boar ejaculates
J. Rungruangsak, J. Suwimonteerabutr, S. Asawakarn, K. Buranaamnuay, N. Chantaravisoot, T.
Pisitkun, and P. Tummaruk
53 Cryopreservation of sheep ovarian tissue using different cryoprotectants
S. Akerke and T. Yerzhan
54 Effect of different cryopreservation protocols for sheep embryonic stem cells
N. Ibraimova, A. Seisenbayeva, and Y. Toishibekov
Developmental Biology
55 The role of poly (ADP-ribose) polymerases in porcine cumulus–oocyte complex during in vitro
maturation and embryonic development
D. H. Kim, S. T. Shin, and H. T. Lee
56 Sirtinol treatment influences preimplatation development of porcine embryos via regulation of
autophagy and apoptosis
M. G. Kim, S. T. Shin, H. D. Shin, and H. T. Lee
57 Disruption of TET1 leads to abnormal expression of pluripotency-related genes in porcine
embryos
K. Uh, J. Ryu, and K. Lee
58 Evidence of metabolic compartmentalization or zonation in the bovine placenta: Significance for
the regulation of placental function and fetal growth
E. Ticiani, C. R. Lazzarotto, R. P. C. Gerger, B. R. Wilhelm, V. H. V. Rodrigues, J. L. Rodrigues,
M. A. Miglino, L. R. Bertolini, and M. Bertolini
59 Selected reaction monitoring-based absolute quantification of developmentally relevant proteins
in early bovine embryos reveals differences between in vitro and in vivo embryo culture and
between different maternal metabolic stages
G. J. Arnold, K. Gegenfurtner, T. Frohlich, D. R. Deutsch, P. Salvetti, N. Forde, P. Lonergan, U.
Besenfelder, and E. Wolf
60 Reproductive performance after timed artificial insemination followed by timed embryo transfer
of in vitro-produced embryos in beef cattle
G. Holguin-Sanabria, F. J. F. Collares, E. P. Silva, L. H. Aguiar, P. Rodriguez-Villamil, F. L.
Ongaratto, P. V. Marchioretto, M. C. Silveira, J. L. Rodrigues, and M. Bertolini

61 Expression profiles and functional roles of H3.3 and HIRA in bovine early embryos
K. Zhang and H. Wang
62 Will gonadotropin-releasing hormone treatment hasten the onset of puberty in peripubertal
heifers?
N. A. Castro, C. E. Leonardi, E. M. Zwiefehofer, J. Singh, and G. P. Adams
63 Impact of endometrial biopsy on corpus luteum function
O. Ramirez-Garzon, N. Satake, J. Hill, M. K. Holland, and M. McGowan
64 Impaired post-implantation development following blastomere biopsy is associated with
placental hypomethylation
F. Zacchini, M. Ogluska, R. Arena, and G. E. Ptak
65 Placental function at term is altered in broodmares fed with cereals from mid gestation
M. Robles, P. Peugnet, C. Dubois, F. Piumi, L. Jouneau, M.-C. Aubrière, M. Dahirel, L. Wimel, A.
Couturier-Tarrade, and P. Chavatte-Palmer
66 Unexpected protein dynamics during the oocyte-to-embryo transition in mice: A mass
spectrometry and RNA sequencing tandem study
S. Israel, M. Ernst, O. E. Psathaki, H. C. Drexler, E. Casser, Y. Suzuki, W. Makalowski, G.
Fuellen, M. Boiani, and L. Taher
Early Pregnancy
67 Influence of metabolic status and genetic merit for fertility on proteomic composition of bovine
uterine luminal fluid
K. Gegenfurtner, T. Fröhlich, M. Kösters, E. O. Riedel, S. Fritz, P. Salvetti, N. Forde, P.
Lonergan, E. Wolf, and G. J. Arnold
69 Can bovine sperm interaction with the oviduct cells after artificial insemination affect the
transcriptome profile of the oviduct?
J. O. Carvalho, R. Sartori, P. L. Monteiro, L. O. Leme, and M. A. N. Dode
70 Effect of ovulation stimulus on biochemical composition of the oviduct of alpaca (Vicugna
pacos)
F. Y. Hilari, J. C. Villanueva, W. F. Huanca, B. Lira, and W. Huanca
Embryo Culture
71 The effect of two different in vitro culture media and mice embryo groupings on hatchability
after 24 hours of culture
N. C. Negota, M. L. Mphaphathi, L. P. Nethenzheni, T. L. Rammutla, N. R. Serota, and T. L.
Nedambale
72 Protective effects of C-phycocyanin on the developmental competence of porcine parthenotes
Y.-J. Niu, N.-H. Kim, and X.-S. Cui
73 Fatty acid supplementation in culture medium with reduced nutrient concentrations improves
bovine blastocyst development compared with standard culture medium
R. Pasquariello, J. R. Herrick, Y. Yuan, A. F. Ermisch, J. Becker, L. Yao, C. Broeckling, W. B.
Schoolcraft, J. P. Barfield, and R. L. Krisher
74 Treatment with melatonin during in vitro culture enhances porcine parthenogenetically activated
embryo development
G. A. Kim, J.-X. Jin, S. Lee, A. Taweechaipaisankul, and B. C. Lee
75 Improvement of developmental competence of bovine in vitro-produced embryos by using
charcoal:dextran-stripped fetal bovine serum on in vitro culture media
A. Mesalam, R. Kong, B.-H. Choi, K.-L. Lee, B.-Y. Park, M.-J. Son, J.-I. Jin, and I.-K. Kong

76 Tetrahydrofuran does not have embryo toxic effects on in vitro-produced bovine embryos
M. M. R. Chowdhury, I. Khan, A. Mesalam, K.-L. Lee, J.-Y. Hwang, F. Afrin, and I.-K. Kong
77 Monozygotic twin calves production by blastomere separation technique with commercial
well-of-the-well culture dish
Y. Hashiyada, Y. Aikawa, H. Matsuda, T. Yamanouchi, Y. Goto, M. Ohtake, S. Sugimura, and K.
Imai
78 Mito-TEMPO, a scavenger for mitochondria-derived reactive oxygen species, enhances porcine
pre-implantation embryo development
S.-G. Yang, H.-J. Park, J.-W. Kim, J.-M. Jung, H.-G. Jegal, I.-S. Kim, P.-S. Jeong, and D.-B. Koo
79 Effect of polydatin on development and cryotolerance of in vitro-produced bovine embryos
C. M. Owen, M. Barceló-Fimbres, J. L. Altermatt, and L. F. Campos-Chillon
80 Cumulus cells reflect the success of IVF of porcine oocytes treated with dimethylthiourea
D. M. Lombardo, M. S. Lorenzo, P. R. Cruzans, G. M. Teplitz, A. Maruri, and M. F. Tello
81 Effects of Day 5 or 6 HEPES-buffered transportation culture medium on developmental
competence of bovine in vitro-produced embryos
W. Choi, C. M. Owen, M. Barcelo-Fimbres, J. L. Altermatt, and L. F. Campos-Chillon
Embryo Manipulation
82 Compensation of the growth and development of individually transferred bovine bisected
embryos
E. I. Schild, A. E. Ynsaurralde-Rivolta, S. López-Valiente, S. Maresca, C. Munar, N. Bosetti, M.
Curti, J. I. Jaca, R. Bevacqua, A. Rogberg, and D. F. Salamone
Embryo Transfer
83 Effect of synchronization treatment and estrus expression on conception rates and pregnancy
losses in recipients receiving in vitro-produced embryos
A. Cedeño, P. Tribulo, A. Tribulo, J. L. Barajas, J. Ortega, S. Andrada, D. Lozano, I. Monguillot,
A. Brandan, R. Tribulo, H. Tribulo, and G. A. Bo
84 Effect of embryo stage and cryopreservation method on pregnancy rates obtained following the
transfer of in vivo-derived ovine embryos on small-scale farms in Thailand
S. Khunmanee, J. Suwimonteerabutr, M. Techakumphu, and T. Swangchan-Uthai
85 Influence of short-term storage on gene expression of equine embryos
G. D. A. Gastal, D. Scarlet, R. Ertl, and C. Aurich
86 Difficulty of transfer of in vivo-derived bovine embryos and route of administration of flunixin
meglumine at the time of transfer may affect pregnancy rate
J. Duran, D. Argudo, S. Bravo, C. Soria, G. Guevara, and R. Alberio
87 Effect of treatment with nonsteroidal anti-inflammatory drugs (NSAIDs) on pregnancy rates of
recipient alpacas post-embryo transfer
H. W. Vivanco-Mackie, M. D. P. Salazar, M. Miguel-Gonzales, C. R. Youngs, and M. Asparrin
88 Effect of follicular ablation and GnRH on recipient synchrony in Holstein heifers
J. M. Palomino, F.-X. Grand, C. Vigneault, P. Blondin, and M.-A. Sirard
89 Comparative quantification of plasma progesterone through radioimmumoassay and enzyme-
linked fluorescent assay techniques in cattle
J. M. R. Pérsico, C. Bianchi, C. Tapia, S. Raggio, and I. A. Marchetti

Epidemiology/Diseases
90 Risk of Coxiella burnetii transmission via embryo transfer using in vitro early caprine embryos
F. Fieni, A. Alsaleh, J. de Souza-Fabjan, P. Mermillod, E. Corbin, P. Nascimento, J.-F. Bruyas,
and J.-L. Pellerin
91 Expression pattern of neuron-specific red fluorescence protein in nervous system of transgenic
dog with human synapsin I promoter
H. J. Oh, M. J. Kim, G. A. Kim, E. M. N. Setyawan, S. H. Lee, and B. C. Lee
Exotic Species
92 Sperm cryopreservation in the Burmese python (Python bivittatus) as a model for endangered
snakes
C. Young, N. Ravida, M. Rochford, and B. Durrant
93 Oestrous synchronisation studies in a marsupial
R. R. Witt, L. A. Hinds, and J. C. Rodger
94 Semen parameters from a captive population of the endangered Chacoan peccary (Catagonus
wagneri) in Paraguay
C. C. Goblet, A. E. Newell-Fugate, G. West, and J. M. Campos-Krauer
95 Successful ovum pick-up-in vitro embryo production in Lidia cattle in France
G. Gamarra Lazo, S. Lacaze, and D. Di Scala
96 Follicular fluid and serum biochemical composition in alpacas (Vicugna pacos) with different
nutritional planes
W. Huanca, F. Hilari, M. Ticona, B. Lira, J. C. Villanueva, and W. F. Huanca
97 Social dominance does not affect semen quality in African wild dogs (Lycaon pictus)
F. Van den Berghe, M. C. J. Paris, Z. Sarnyai, M. B. Briggs, R. P. Millar, A. Ganswindt, and D. B.
B. P. Paris
98 Assessing endangered felid Puma concolor sperm fertility by in vitro fertilization with domestic
cat oocytes
M. Duque, A. Sestelo, and D. F. Salamone
99 In vitro development of alpaca embryos obtained by bisection
L. Landeo, R. S. Molina, M. E. Zuñiga, T. R. Gastelu, C. Sotacuro, and J. A. Ruiz
Folliculogenesis/Oogenesis
100 Use of seminal plasma, human chorionic gonadotropin, and follicular ablation on the interval to
follicular wave emergency and dominant follicle in alpacas (Vicugna pacos)
W. F. Huanca, F. Y. Hilari, J. C. Villanueva, M. Uchuari, and W. Huanca
101 Effects of active immunization against GnRH in oocyte donors with cystic ovarian disease
O. A. C. Faria, L. R. O. Dias, L. Leme, G. Fernandes, A. A. G. Fidelis, L. G. B. Siqueira, and J.
H. M. Viana
102 In vitro embryo production and oocyte quality in Bos indicus beef cows selected for fertility
characteristics
G. L. Vasconcelos, R. Maculan, N. Alves, A. L. A. P. L. Ribeiro, A. W. B. Silva, E. V. Cunha, G. M.
Moreira, J. A. S. Viafara, A. S. B. Guimaraes, C. V. Ribeiro, J. R. V. Silva, J. N. S. Sales, T. L. C.
Pinto, M. S. El Azzi, and J. C. DeSouza
103 The tunica albuginea increases in thickness and density correlatively with the age of the patient in
human ovaries
P. Ferré, J. Otsuki, O. Takayama, H. Motohashi, T. Wakai, M. Nakatsuka, and H. Funahashi

104 Distribution of gonadotropin-releasing hormone and kisspeptin neurons in the preoptic area and
hypothalamus during the estrous cycle in cows
C. E. P. Leonardi, R. Carrasco, F. F. C. Dias, G. P. Adams, and J. Singh
105 P75 neuronal cells and fibers in the bovine ovary
R. Carrasco, C. E. Leonardi, J. Singh, and G. P. Adams
106 Effect of heat-stress on antral follicle count and serum concentration of anti-Mullerian hormone
in Bos taurus crossbred beef cows
F. A. Diaz, E. J. Gutierrez, B. A. Foster, P. T. Hardin, and K. R. Bondioli
107 Localization of kisspeptin immunoreactivity in the cat ovary on different reproductive stages
P. Tanyapanyachon, O. Amelkina, and K. Chatdarong
Gene Expression
108 Single-cell RNA sequencing reveals blastomere heterogeneity and early lineage specification
events in bovine embryos during major embryonic genome activation
I. Lavagi, S. Krebs, K. Simmet, V. Zakhartchenko, E. Wolf, and H. Blum
109 Dosage compensation of the X chromosome in ovine embryos, late gestation, and adult somatic
tissues
J. Duan, K. Flock, M. Zhang, A. K. Jones, S. M. Pillai, M. L. Hoffman, H. Jiang, S. A. Zinn, S. A.
Reed, K. E. Govoni, N. K. Jue, R. O’Neill, Z. Jiang, and X. Tian
110 Temporal changes in endometrial gene expression between ipsi- and contralateral uterine horns in
cattle
J. M. Sánchez, C. Passaro, N. Forde, S. Behura, J. A. Browne, D. J. Mathew, S. T. Butler, T. E.
Spencer, and P. Lonergan
111 Inhibition of avian influenza virus by blocking specific sialyltransferases
E. N. Antonova, O. V. Glazova, A. V. Gaponova, N. A. Volkova, and P. Y. Volchkov
112 Identification of solute carrier family 5 member 9 (SLC5A9) as biomarker of in vivo and in vitro
oocyte developmental competence
E. F. M. Manzoni, V. Bocchi, C. Galli, M. G. Strillacci, G. Stradaioli, T. A. L. Brevini, and F.
Gandolfi
113 Specific impacts of mild feed restriction on gene expression of endometrial luminal, glandular
and stromal cells in postpartum dairy cows
W. Chankeaw, S. Lignier, M. Raliou, T. Ntallaris, C. Richard, Y. Guo, O. Sandra, G. Andersson, P.
Humblot, and G. Charpigny
114 Insulin-like growth factor (IGF)2 and IGF2 receptor (IGF2R) murine blastocyst transcriptional
response after high gaseous pressure exposure at 8-cell stage
B. S. Becker, F. J. F. Collares, A. V. Gonsiorosky, C. Rodrigues de Freitas, D. A. Mentz, L. R.
Bertolini, M. Bertolini, and J. L. Rodrigues
115 In vivo transcriptomic response of bovine oviduct epithelial cells to the early embryo
B. Rodríguez-Alonso, M. Hamdi, J. M. Sánchez, A. Gutierrez-Adán, P. Lonergan, and D. Rizos
116 In vitro transcriptomic response of bovine oviduct epithelial cells to direct or indirect embryo
contact
M. Hamdi, B. Rodríguez-Alonso, A. Almansa-Ordonez, A. Gutierrez-Adán, P. Lonergan, and D.
Rizos
117 Subclinical mastitis reduces ovulation and oocyte quality in milk-producing cows
G. Santos, M. P. Bottino, M. B. D. Ferreira, J. C. Silveira, A. C. F. C. M. Avila, A. Bridi, J. C.
DeSouza, and J. N. S. Sales

118 Identification of KISS1 in the domestic cat
O. Amelkina, P. Tanyapanyachon, and K. Chatdarong
119 Genetic highlights for reproduction and health traits in Russian Black-and-White and Holstein
animals selected for production of high-quality embryos
A. A. Sermyagin, A. F. Conte, V. V. Volkova, O. S. Romanenkova, A. A. Kharzhau, H. Reyer, K.
Wimmers, G. Brem, and N. A. Zinovieva
IVF/IVP
120 Theophylline: The key for bulls with low in vitro blastocyst rates?
S. M. Bernal-Ulloa and S. E. Ulbrich
121 Comparing two superstimulation protocols prior to ovum pick-up in dromedary camel
T. Ararooti, A. Niasari-Naslaji, and K. Razavi
122 A new maturation medium improves porcine embryo production in vitro
A. Lucas-Hahn, B. Petersen, M. Nowak-Imialek, U. Baulain, R. Becker, H.-M. Eylers, K.-G.
Hadeler, P. Hassel, and H. Niemann
123 In vitro embryo production in lyophilized in vitro culture medium as a method to increase the
medium’s shelf-life
M. Rubessa, F. Salerno, D. Weisgerber, B. Gasparrini, B. Harley, and M. Wheeler
124 Cell count of bovine in vitro-produced blastocysts after in vitro maturation in glass versus plastic
vials
J. O. Secher, N. Hashem, J. H. Pryor, C. R. Long, J. Docherty, B. M. Avery, M. Ivask, P. Hyttel,
and L. B. Strøbech
125 Effect of complexed trace minerals on oocyte and embryo production
F. G. Dantas, R. V. O. Filho, R. S. Carvalho, G. A. Franco, S. T. Reese, C. R. Abbott, R. R.
Payton, J. L. Edwards, J. K. Smith, J. R. Russell, and K. G. Pohler
126 A comparison of two different follicular coasting periods for in vitro embryo production in Indian
Nelore cattle
P. Tiwari, S. Zawar, J. H. Pryor, C. R. Looney, R. Kaushik, A. Sahare, and J. Hazarika
127 l-Carnitinesupplementation during in vitro maturation improves developmental competence of
canine oocytes
A. Salama, M. Fathi, M. R. Badr, and A. R. Moawad
128 Evidence that pregnancy-associated serum protein A (PAPP-A) plays role on bovine in vitro
embryo production
A. B. Giroto, F. F. Franchi, P. K. Fontes, M. A. Maioli, G. P. Nogueira, M. F. G. Nogueira, and A.
C. S. Castilho
129 Embryo production from fully grown and growing stage oocytes in Japanese Black calves
S. Matoba, K. Takeda, Y. Ohkubo, M. Hirako, and Y. Hirao
130 In vitro production of Hybrid Desert Bighorn × Domestic sheep embryos using frozen-thawed
epididymal semen from a hunter-harvested ram
M. G. Licea, J. E. H. Pichardo, J. L. Rodríguez, A. García-Contreras, B. C. Rosales, M. Palma-
Irizarry, S. Romo, and M. E. Kjelland
131 Effect of oviductal fluid during in vitro culture on bovine embryo development and quality
R. Emmerstorfer, K. Radefeld, V. Havlicek, U. Besenfelder, H. Yu, C. Mayrhofer, C. Vogl, G.
Brem, and S. Papp
132 The developmental competence of bovine oocytes exposed to progesterone and luteotrophic
hormones during the second step of two-step maturation
G. Singina, I. Lebedeva, T. Taradajnic, E. Shedova, A. Lopukhov, and N. Zinovieva

133 In vitro embryo production from oocytes collected from non-superstimulated wood bison (Bison
bison athabascae) following maturation in vitro using portable incubators
M. P. Cervantes, G. P. Adams, M. Anzar, J. M. Palomino, and G. F. Mastromonaco
134 Effects of gas tension during culture upon development of bovine embryos from beef (Nellore)
and dairy (Girolando) breeds
J. G. V. Grázia, L. G. Lacerda, L. G. B. Siqueira, C. A. G. Pellegrino, L. S. Grapiuna, N. V.
Sollecito, and J. H. M. Viana
135 Effect of different bovine breeds on efficiency of embryo production by ovum pick-up-IVF
S. Sato, M. Moriyoshi, O. Dochi, and K. Imai
136 DNA methylation status and transcript profile of genes associated with lipid metabolism, cell
survival, and pluripotency in bovine blastocysts with different kinetics of development
J. Ispada, C. Bruna de Lima, E. Cristina dos Santos, K. Annes, P. K. Fontes, M. F. G. Nogueira,
M.-A. Sirard, and M. P. Milazzotto
137 Kinetics of in vitro embryonic development according to the follicular population in Bos indicus
J. G. Soares, F. M. Morato, G. F. Rossi, B. M. Bayeux, A. S. Oliveira, O. Y. Watanabe, J. N. S.
Sales, E. G. Lo Turco, M. F. G. Nogueira, P. K. Fontes, Y. F. Watanabe, and P. S. Baruselli
138 Relationships between antral follicle count, ovarian volume, pre-antral follicle number, and
oocyte quality
G. L. Vasconcelos, R. Maculan, N. Alves, A. L. A. P. L. Ribeiro, A. W. B. Silva, E. V. Cunha, G. M.
Moreira, J. A. S. Viafara, A. S. B. Guimaraes, C. V. Ribeiro, J. R. V. Silva, T. L. C. Pinto, J. N. S.
Sales, and J. C. DeSouza
Male Physiology
139 Sperm transmembrane protein 95 (TMEM95) is required for sperm-oocyte interaction and
successful fertilization but not mucus penetration in cattle
B. Fernandez-Fuertes and P. Lonergan
140 Endocrine profiling in prepubertal stallions with abnormal testicular development
D. Scarlet, J. Kuhl, M. Wulf, N. Ille, M. Köhne, A. Conley, and C. Aurich
141 Antioxidant supplementation alleviates DNA damage in boar sperm induced by tropical heat
stress
S. T. Peña Jr., B. Gummow, A. J. Parker, and D. B. B. P. Paris
142 Isolation of the quail spermatogonia
E. R. Mennibaeva, N. A. Volkova, E. K. Tomgorova, L. A. Volkova, V. A. Bagirov, and N. A.
Zinovieva
143 The dynamics of spermatogenesis in guinea fowls
N. A. Volkova, A. N. Vetokh, I. P. Novgorodova, A. V. Dotsev, and N. A. Zinovieva
144 Dynamic of sperm subpopulations in red deer capacitated samples
M. Ramón, M. Iniesta-Cuerda, A. Martín-Maestro, P. Peris-Frau, I. Sánchez-Ajofrín, M. R.
Fernández-Santos, J. Garde, and A. J. Soler
145 Effect of dietary organic zinc levels on boar (Sus scrofa domesticus) sperm DNA fragmentation
dynamics
A. Martínez, J. Gosálvez, C. López-Fernández, J. A. G. González, C. G. Artiga, Y. D. L. Ortega,
M. E. Kjelland, and A. García-Contreras
146 Effect of pH on rainbow trout (Oncorhynchus mykiss) sperm motility using five extender
solutions
M. Á. Peralta-Martínez, S. R. García, M. E. Kjelland, and H. González-Márquez

147 Dietary zinc and its effect on collection time and training quality of young boars for obtaining
ejaculates
Y. D. L. Ortega, C. G. Artiga, J. A. G. González, A. P. Yagüe, B. I. Redondo, M. E. Kjelland, and
A. C. García-Contreras
148 Inmunolocalization of β-nerve growth factor (NGF) in male reproductive tract and NGF levels in
serum and seminal plasma at puberty and adulthood in rabbit
A. Sanchez-Rodriguez, M. Arias-Alvarez, P. G. Rebollar, P. L. Lorenzo, and R. M. Garcia-Garcia
149 Preliminary results of the oxytocin treatment on the collection of cat semen by urethral catheter-
ization after pharmacological induction
F. Cremonesi, M. Mussi, C. Perrini, and A. Lange-Consiglio
150 Runs of homozygosity analysis and their possible influence on sperm motility in highly consan-
guineous bulls
E. Terán, D. Goszczynski, A. Molina, P. Ross, J. Dorado, G. Giovambattista, and S. D. Peyrás
151 Associations of testicular size and arterial blood flow with sexual development and semen
characteristics in Dorper rams raised in a subtropical climate
E. S. C. Camela, R. P. Nociti, V. J. C. Santos, B. I. Macente, G. S. Maciel, M. A. R. Feliciano, W.
R. R. Vicente, I. Gill, P. M. Bartlewski, and M. E. F. Oliveira
Oocyte Activation
152 Activation of bovine oocytes using the zinc chelator TPEN
C. L. Timlin, K. Uh, V. R. G. Mercadante, and K. Lee
153 Fertilisation of cattle oocytes is linked to novel waves of a Ca2+ fluorophore in cumulus cells
H. J. McLennan, M. L. Sutton-McDowall, S. Heng, and J. G. Thompson
Oocyte Maturation
154 Exosomes in follicular fluid protect the bovine oocyte from heat shock
T. A. Rodrigues, A. Alli, F. F. Paula-Lopes, and P. Hansen
155 Oocyte maturation in lyophilized in vitro maturation medium as a method to increase the
medium’s shelf-life
M. Rubessa, D. Weisgerber, S. Bessler, J. Bertels, B. Harley, and M. Wheeler
157 Sodium fluoride exposure exerts toxic effects on porcine oocyte maturation
Z.-W. Nie, S. Liang, and X.-S. Cui
158 The effect of follicular wave phase at time of ovum pick-up on bovine oocyte cytoplasmic
maturation and developmental competence
B. A. Foster, F. A. Diaz, E. J. Gutierrez, and K. R. Bondioli
159 Effect of commercial embryo holding medium on development and quality of immature bovine
oocytes
M. Catteeuw, O. B. Pascottini, G. Opsomer, and A. Van Soom
160 Exposure to cadmium affects oocyte and embryo competence in cattle
C. De Canditiis, N. Pagano, V. Franco, I. Paradiso, É. C. Dos Santos, G. Zullo, G. Albero, and B.
Gasparrini
161 Cumulus cell luteinization is enhanced during aging of bovine oocytes matured in vitro
I. Y. Lebedeva, G. N. Singina, S. V. Uzbekova, P. Papillier, E. N. Shedova, A. V. Lopukhov, and O.
S. Mityashova

162 Supplementation of fibroblast growth factor 2, leukemia inhibitory factor, and insulin-like growth
factor 1 (FLI) enables efficient production of competent porcine oocytes without gonadotropins
in the maturation medium
Y. Yuan, L. D. Spate, B. K. Redel, R. S. Prather, and R. M. Roberts
163 Treatment of growth differentiation factor 8 (GDF8) during porcine oocyte in vitro maturation
changed transcriptional pattern and AKT signaling
J. D. Yoon, E. Lee, and S.-H. Hyun
164 The origin of oocyte, in vitro-matured oocyte with/without super-stimulation, and in vivo-matured
oocyte influence the timing of cleavage in early embryo and oxygen consumption of blastocyst
after IVF in Japanese Black cow
T. Yamanouchi, H. Matsuda, M. Ohtake, Y. Ogata, Y. Aikawa, and Y. Hashiyada
165 Effect of dimethyl sulfoxide supplementation during in vitro maturation on the genetic expression
pattern of bovine blastocyst
A. E. Ynsaurralde-Rivolta, M. Suvá, R. Bevacqua, L. Rodriguez-Alvarez, A. Velasquez, O. Briski,
and D. Salamone
166 Paracrine regulation of epidermal growth factor-like factors in oviduct cells and its effect on
oocyte maturation and subsequent embryo development
S. H. Lee, E. M. N. Setyawan, and B. C. Lee
167 Effect of storage time and temperature of a commercial embryo holding medium on the
maturation kinetics of immature bovine oocytes
O. B. Pascottini, M. Catteeuw, A. Van Soom, and G. Opsomer
168 The effect of prematuration culture using epidermal growth factor receptor kinase inhibitor on
nuclear maturation of bovine oocytes and cumulus cell gene expression
G. Mingoti, P. Dall’Acqua, G. Nunes, C. Silva, P. Fontes, and M. Nogueira
169 Nuclear maturation kinetics and in vitro fecundation of immature bovine oocytes injected into
preovulatory follicles
L. M. S. Simoes, A. P. C. Santos, E. A. Lima, R. E. Orlandi, M. P. Bottino, T. T. Pontes, R. C.
DeOliveira, L. O. Silva, S. A. D. Rodrigues, C. C. R. Quintao, M. A. N. Dode, L. S. A. Camargo,
U. R. Fernandes, J. C. DeSouza, J. N. S. Sales
170 Effect of folic acid supplementation on in vitro maturation of oocytes and folate cycle
S. Saini, V. Sharma, A. Kumar, A. Thakur, K. K. Bajwa, and D. Malakar
171 Protective effect of sodium pyruvate against H2O2-induced oxidative stress in bovine and swine
oocytes
I. Daverio, M. Barandalla, C. Galli, and G. Lazzari
172 Effect of melatonin on meiosis progression and lipid content in bovine oocytes matured in vitro
H. Fernandes, L. Schefer, F. C. Castro, D. M. Paschoal, R. C. Botigelli, and C. L. V. Leal
173 Size matters: A follicle-of-origin effect on the preovulatory epigenetic constitution of germinal
vesicle-stage porcine oocytes
S. Heywood, R. L. Matheson, J. Thomas, L. Moley, and S. C. Isom
174 Effects of melatonin on in vitro maturation of bovine oocytes and gene expression in cumulus
cells: Preliminary results
F. C. Castro, L. Schefer, K. L. Schwarz, H. Fernandes, R. C. Botigelli, D. M. Paschoal, and C. L.
V. Leal
Sexing
175 Testis-specific protein Y encoded copy number variation in in vitro-produced bovine early
embryos
N. Y. Rho, L. A. Favetta, T. Mogas, and W. A. King
Sperm Injection
176 Localization and quantitative expression of phospholipase C zeta in equine sperm using
commercial antibodies
R. A. Gonzalez-Castro, J. K. Graham, and E. M. Carnevale
177 Equine sperm decondensation and blastocyst formation after conventional versus piezo-driven
intracytoplasmic sperm injection
J. G. Brom-de-Luna, R. M. Salgado, H. L. Resende, H. S. Canesin, and K. Hinrichs
Stem Cells
178 Differential behavior of porcine mesenchymal stem cells from bone marrow and adipose during
osteogenic differentiation on a glycosaminoglycan hydrogel scaffold for bone and cartilage tissue
engineering
S. A. Womack, D. J. Milner, D. W. Weisgerber, B. A. Harley, and M. B. Wheeler
179 Xenogeneic and allogeneic mesenchymal stem cell transplantation for treatment of tibial bone
fracture in mice
K. K. Bajwa, V. Sharma, S. Saini, A. Kumar, A. Thakur, S. De, S. Kumar, and D. Malakar
180 Increasing GfrA1-positive spermatogonial stem cell population of goat
V. Sharma, S. Saini, B. Aneja, A. Kumar, A. Thakur, K. K. Bajwa, S. Kumar, A. K. Mohanty, and
D. Malakar
181 Establishment of induced pluripotent stem cells from fishing cat and clouded leopard using
integration-free method for wildlife conservation
W. Sukparangsi, R. Bootsri, W. Sikeao, S. Karoon, and A. Thongphakdee
182 In vitro derivation of male germ cells from bovine bone marrow-derived mesenchymal stem cells
J. Cortez, J. Bahamonde, J. Palomino, M. De los Reyes, C. Torres, and O. Peralta
183 In vitro generation and characterization of putative primordial germ cells derived from induced
pluripotent stem cells in cattle
F. F. Bressan, M. A. Lima, L. S. Machado, N. C. G. Pieiri, P. Fantinato-Neto, J. Therrien, F.
Perecin, L. C. Smith, and F. V. Meirelles
184 Establishment of porcine induced pluripotent stem cell lines by adding LIN 28 transcription
factor
S. Rungarunlert, W. Chakritbudsabong, S. Pamonsupornvichit, L. Sariya, R. Pronarkngver,
S. Chaiwattanarungruengpaisan, J. N. Ferreira, P. Setthawonge, P. Phakdeedindan, M.
Techakumphu, and T. Tharasanit
185 Directed differentiation of porcine induced pluripotent stem cells into all three germ layers via
embryoid body formation
W. Chakritbudsabong, S. Pamonsupornvichit, L. Sariya, R. Pronarkngver, S.
Chaiwattanarungruengpaisan, S. Klinsrithong, J. N. Ferreira, and S. Rungarunlert
186 Improving success rate of establishment and maintenance of porcine induced pluripotent stem
cells cells by investigation of colony morphology
P. Setthawong, P. Phakdeedindan, M. Techakumphu, and T. Tharasanit
187 Morphological appearance and expression of spermatogonial stem cell markers in white
rhinoceros testicular tissue
M. C. Gomez, Y. Cates, D. B. Stansfield, C. Young, R. Klee, and B. Durrant
188 The effect of vitamin B12 on the differentiation of adipose-derived stem cells into osteoblasts
T. A. Bane, J. C. Bertels, K. M. Polkoff, M. Rubessa, and M. B. Wheeler
189 The effect of vitamin K on the differentiation of adipose-derived stem cells into osteoblasts
T. A. Bane, J. C. Bertels, K. M. Polkoff, M. Rubessa, and M. B. Wheeler

190 Profile of microRNAs of equine amniotic mesenchymal cell-derived microvesicles in different
time-span of in vitro culture
A. Lange-Consiglio, E. Capra, F. Pizzi, B. Lazzari, C. Perrini, A. Stella, and F. Cremonesi
191 Canine mesenchymal stem cells as therapeutic agents in spinal cord injured dog
Y.-H. Choe, H. Lee, S.-L. Lee, J. Lee, B.-W. Park, and G.-J. Rho
192 Culture conditions supporting long-term expansion of bovine spermatogonial stem cells isolated
from adult and immature testes
Suyatno, Y. Kitamura, N. Minami, M. Yamada, and H. Imai
Superovulation
193 Comparison of superovulatory responses to single subcutaneous FSH injection and twice-daily
intramuscular FSH injection protocols in Japanese Black cows
A. Miyoshi, M. Sugawara, Y. Kaneda, H. Sekizawa, and O. Dochi
194 Usefulness of the ultrasound imaging modalities B-mode and colour Doppler sonography and
serum progesterone concentrations for determining superovulatory ovarian responses in ewes
I. F. Ribeiro, M. G. K. Rodrigues, G. S. Maciel, J. F. Fonseca, F. Z. Brandão, P. M. Bartlewski,
and M. E. F. Oliveira
195 Are the spectral Doppler indices of the ovarian arteries predictive of ovulatory responses and
embryo yields in superovulated ewes?
M. E. F. Oliveira, Y. Tarasevych, W. R. R. Vicente, and P. M. Bartlewski
196 Fertilization rate in superovulated Criolla goats following artificial insemination or natural
mating
M. I. Cueto, J. Fernandez, M. M. Bruno-Galarraga, F. Pereyra-Bonnet, and A. Gibbons
197 The effect of gonadotropin-releasing hormone agonist on superovulation and estrous synchroni-
zation in female Sprague Dawley rat
Y.-K. Jin, H.-S. Bae, J.-Y. Lee, S.-Y. Yum, K.-M. Kim, O.-J. Koo, G. Jang, and M. J. Ailia
Transgenesis
198 Germline-specific expression of the murine Oct4-EGFP transgene in the pig
M. Nowak-Imialek, D. Herrmann, A. Frenzel, and H. Niemann
199 Efficient knock-out of ovine β-lactoglobulin (BLG) gene and knock-in of recombinant human
factor IX (rhFIX) under BLG native regulatory sequences in somatic cells and zygotes using
TALEN nucleases
R. J. Bevacqua, D. Carlson, R. Fernandez-Martín, A. E. Gibbons, V. Savy, N. G. Canel, G. V.
Landschoot, L. De La Rosa, F. Lange, V. Alberio, O. Briski, M. I. Gismondi, S. Ferraris, S.
Fahrenkrug, and D. Salamone
200 Optimization of individual stages of chicken transgenesis to increase efficiency
E. K. Tomgorova, E. N. Antonova, N. A. Volkova, P. Y. Volchkov, and N. A. Zinovieva
201 Spermatogonia transplantation in the chicken
A. N. Vetokh, N. A. Volkova, T. O. Kotova, E. N. Antonova, A. V. Dotsev, and N. A. Zinovieva
202 Targeting galactosyl-α-1,3-galactose (αGal) epitopes for multi-species embryo immunosurgery
M. Kurome, A. Baehr, K. Simmet, B. Kessler, E. Jemiller, M. Dahlhoff, V. Zakhartchenko, N.
Klymiuk, and E. Wolf
203 Genome modifications by Sleeping Beauty transposition and CRISPR/Cas9 to improve cow milk
composition for human consumption
A. Alessio, E. Pericuesta, I. Llamas-Toranzo, D. Forcato, A. Fili, C. Liaudat, N. Rodriguez, W.
Kues, P. Bermejo-Álvarez, and P. Bosch

204 Blood analysis of Cas9-expressing cattle
S.-Y. Yum, S.-J. Lee, S.-E. Hahn, C.-I. Lee, H.-S. Kim, H.-J. Kim, Y. Jin, W.-J. Choi, J.-H. Lee,
W.-S. Lee, and G. Jang
Undergraduate Poster Competition

205 Optimization of transfection efficiency for CRISPR/Cas9-induced genomic editing in porcine
fibroblast cells
S. N. Lanjewar and K. R. Bondioli
206 Effect of L-ascorbic acid, polydatin, and in-straw rehydration of slow-frozen in vitro-produced
bovine embryos
C. M. Dinndorf, C. M. Owen, M. Barceló-Fimbres, and L. F. Campos-Chillon
207 Investigating the cryotolerance of boar spermatozoa subjected to prior selection
M. B. Lewis, C. L. Durfey, S. K. Hartung, C. S. Steadman, S. Park, H. J. Clemente, S. T. Willard,
P. L. Ryan, and J. M. Feugang
208 Cryotolerance of in vitro-produced cattle embryos produced with different maturation and culture
media
M. A. Taylor, C. M. Owen, M. Barceló-Fimbres, and L. F. Campos-Chillon
DABE Forum
209 Imaging the molecular and cell dynamics that form the early mouse embryo
N. Plachta

Author Index
Author, Poster = abstract number in Reproduction, Fertility and Development 2018; 30 (1)
Bajwa, K. K., 170, 179, 180 Bravo, S., 86

Abbott, C. R., 125 Balvís, N. F., 6 Brem, G., 119, 131
Adams, G. P., 3, 4, 12, 62, 104, 105, Bane, T. A., 188, 189 Bressan, F. F., 183
133 Barajas, J. L., 83 Brevini, T. A. L., 112
Adel, H., 22 Barandalla, M., 171 Bridi, A., 117
Afrin, F., 76 Barceló-Fimbres, M., 79, 81, 206, Briggs, M. B., 97
Aguiar, L. H., 31, 60 208 Briski, O., 165, 199
Aikawa, Y., 77, 164 Barfield, J. P., 5, 73 Broeckling, C., 73
Ailia, M. H., 197 Barros, P. A. M., 13 Brom-de-Luna, J. G., 51, 177
Akerke, S., 53 Bartlewski, P. M., 151, 194, 195 Browne, J. A., 110
Alberio, R., 86 Baruselli, P. S., 13, 137 Bruna de Lima, C., 136
Alberio, V., 199 Baulain, U., 122 Bruno-Galarraga, M. M., 196
Albero, G., 160 Bayeux, B. M., 137 Bruyas, J.-F., 90
Alessio, A., 203 Becker, B. S., 114 Buemo, C. P., 26
Alli, A., 154 Becker, J., 73 Buranaamnuay, K., 52
Almansa-Ordonez, A., 116 Becker, R., 122 Butler, S. T., 110
Alsaleh, A., 90 Behura, S., 110 Callesen, H., 23
Altermatt, J. L., 79, 81 Benham, H., 5 Camargo, L. S. A., 169
Alves, N., 102, 138 Bentzon, J. F., 23 Camela, E. S. C., 151
Amelkina, O., 107, 118 Bermejo-Álvarez, P., 6, 203 Campos-Chillon, L. F., 79, 81, 206,
Andersson, G., 113 Bernal-Ulloa, S. M., 120 208
Ando, T., 43 Bernardi, M., 11 Campos-Krauer, J. M., 94
Andrada, S., 83 Bertels, J. C., 155, 188, 189 Canel, N., 26
Aneja, B., 180 Bertolini, L. R., 31, 58, 114 Canel, N. G., 24, 199
Annes, K., 136 Bertolini, M., 31, 58, 60, 114 Canesin, H. S., 51, 177
Antonova, E. N., 111, 200, 201 Berton, T. I. H., 9 Capra, E., 190
Anzalone, D. A., 45 Berton, T. I. U., 8, 10 Carlson, D., 199
Anzar, M., 3, 133 Besenfelder, U., 59, 131 Carnevale, E. M., 176
Ararooti, T., 22, 121 Bessler, S., 155 Carrasco, R., 104, 105
Arav, A., 47 Bevacqua, R., 82, 165 Carvalho, J. O., 69
Arcarons, N., 40 Bevacqua, R. J., 24, 199 Carvalho, R. S., 125
Arena, R., 64 Bianchi, C., 89 Casser, E., 66
Argudo, D., 86 Binoy, V. S., 34 Castañeda, F. K., 24
Arias-Alvarez, M., 148 Blondin, P., 88 Castilho, A. C. S., 128
Arnold, G. J., 59, 67 Blum, H., 108 Castro, F. C., 172, 174
Arnold, V., 29, 30 Bo, G. A., 83 Castro, N. A., 62
Artiga, C. G., 145, 147 Bocchi, V., 112 Cates, Y., 187
Asanova, E., 39 Boiani, M., 66 Catteeuw, M., 159, 167
Asawakarn, S., 52 Bondioli, K. R., 41, 48, 106, 158, Caunce, S. L., 4
Asparrin, M., 87 205 Cedeño, A., 83
Aubrière, M.-C., 65 Bootsri, R., 181 Cervantes, M. P., 133
Aurich, C., 85, 140 Bopape, M. A., 16 Chaikhun-Marcou, T., 19
Avery, B. M., 124 Borges, J. B., 11 Chaiwattanarungruengpaisan, S.,
Avila, A. C. F. C. M., 117 Bosch, P., 203 184, 185
Avilés, M., 6 Bosetti, N., 82 Chakritbudsabong, W., 184, 185
Badr, M. R., 49, 127 Botigelli, R. C., 172, 174 Chankeaw, W., 113
Bae, H.-S., 197 Bottino, M. P., 13, 117, 169 Chantaravisoot, N., 52
Baehr, A., 202 Bovula, N., 20, 21 Charpigny, G., 113
Bagirov, V. A., 142 Brandan, A., 83 Chatdarong, K., 44, 107, 118
Bahamonde, J., 182 Brandão, F. Z., 194 Chavatte-Palmer, P., 65
Chimonyo, M., 21 Dotsev, A. V., 143, 201 Fritz, S., 67
Choe, Y.-H., 191 Drexler, H. C., 66 Fröhlich, T., 59, 67
Choi, B.-H., 75 Duan, J., 109 Fuellen, G., 66
Choi, K.-D., 25 Dubois, C., 65 Fujikawa, T., 43
Choi, W., 81 Duque, M., 98 Funahashi, H., 103
Choi, W.-J., 204 Duran, J., 86 Galli, C., 35, 112, 171
Choi, Y.-H., 51 Durfey, C. L., 207 Gambini, A., 26
Chowdhury, M. M. R., 76 Durrant, B., 92, 187 Gamarra Lazo, G., 95
Clemente, H. J., 207 Edwards, J. L., 125 Gandolfi, F., 112
Collares, F. J. F., 60, 114 El Azzi, M. S., 102 Ganswindt, A., 97
Conley, A., 140 Eliab, E., 2 Gaponova, A. V., 111
Conte, A. F., 119 Emmerstorfer, R., 131 García, S. R., 146
Corbin, E., 90 Ermisch, A. F., 73 García-Contreras, A., 130, 145
Cortez, J., 182 Ernst, M., 66 García-Contreras, A. C., 147
Couturier-Tarrade, A., 65 Ertl, R., 85 Garcia-Garcia, R. M., 148
Cremonesi, F., 149, 190 Eylers, H.-M., 122 Garde, J., 144
Cristina dos Santos, E., 136 Fahrenkrug, S., 199 Garry, D. J., 25
Cruz, T. E., 8, 9, 10 Fan, Z., 1, 28 Garry, M. G., 25
Cruzans, P. R., 80 Fantinato-Neto, P., 183 Gasparrini, B., 123, 160
Cueto, M. I., 196 Faria, O. A. C., 101 Gastal, G. D. A., 85
Cui, X.-S., 32, 72, 157 Fathi, M., 49, 127 Gastelu, T. R., 99
Cunha, E. V., 102, 138 Favetta, L. A., 175 Gegenfurtner, K., 59, 67
Curti, M., 82 Feliciano, M. A. R., 151 Gen, Y., 43
Dahirel, M., 65 Fernandes, G., 101 Gerger, R. P. C., 58
Dahlhoff, M., 202 Fernandes, H., 172, 174 Gibbons, A., 196
Dall’Acqua, P., 168 Fernandes, U. R., 169 Gibbons, A. E., 199
Dantas, F. G., 125 Fernandez, J., 196 Gill, I., 151
Das, S., 25 Fernandez-Fuertes, B., 139 Giovambattista, G., 150
DaSilva, L. A. C. L., 13 Fernandez-Martín, R., 199 Giroto, A. B., 128
Daverio, I., 171 Fernández-Santos, M. R., 144 Gismondi, M. I., 199
De, S., 179 Ferraris, S., 199 Glazova, O. V., 111
De Canditiis, C., 160 Ferré, P., 103 Goblet, C. C., 94
De La Rosa, L., 199 Ferreira, J. N., 184, 185 Gomes dos Santos, G. M., 7
De los Reyes, M., 182 Ferreira, M. B. D., 117 Gomez, M. C., 187
de Souza-Fabjan, J., 90 Feugang, J. M., 207 Gonsiorosky, A. V., 114
De Stéfano, A., 24 Fichtner, T., 14 González, J. A. G., 145, 147
DeOliveira, R. C., 169 Fidelis, A. A. G., 101 Gonzalez-Castro, R. A., 176
DeSouza, J. C., 13, 102, 117, 138, Fieni, F., 90 González-Márquez, H., 146
169 Filho, R. V. O., 125 Gosálvez, J., 145
Deutsch, D. R., 59 Fili, A., 203 Gosálvez´, J., 45
Di Scala, D., 95 Flock, K., 109 Goszczynski, D., 150
Dias, F. C. F., 4 Fonseca, J. F., 194 Goto, Y., 77
Dias, F. F. C., 104 Fontes, P., 168 Govoni, K.E., 109
Dias, L. R. O., 101 Fontes, P. K., 128, 136, 137 Graham, J. K., 176
Dias, M., 11 Forcato, D., 203 Grand, F.-X., 88
Diaz, F. A., 41, 48, 106, 158 Forde, N., 59, 67, 110 Grapiuna, L. S., 134
Dinndorf, C. M., 206 Foster, B. A., 41, 48, 106, 158 Grázia, J. G. V., 134
Dobler, D., 30 Franchi, F. F., 128 Guevara, G., 86
Docherty, J., 124 Franco, G. A., 125 Guimaraes, A. S. B., 102, 138
Dochi, O., 135, 193 Franco, V., 160 Guiterrez, E. J., 41
Dode, M. A. N., 69, 169 Freitas-Dell’Aqua, C. P., 8, 10 Gummow, B., 141
Dorado, J., 150 Frenzel, A., 198 Guo, Y., 113
Dos Santos, É. C., 160 Frey, B., 5 Gutierrez, E. J., 48, 106, 158

Gutierrez-Adán, A., 115, 116 Jiang, H., 109 Koyano-Nakagawa, N., 25
Hadeler, K.-G., 122 Jiang, Z., 109 Krause, A. R. T., 4
Hahn, S.-E., 204 Jin, J.-I., 75 Krebs, S., 108
Hamdi, M., 115, 116 Jin, J.-X., 27, 74 Krisher, R. L., 73
Hansen, P. J., 2, 154 Jin, Y.-., 204 Kruger, L. P., 17
Hardin, P. T., 106 Jin, Y.-K., 197 Kubota, C., 43
Harley, B., 123, 155 Jones, A.K., 109 Kues, W., 203
Harley, B. A., 178 Joo, M.-D., 38 Kuhl, J., 140
Hartung, S. K., 207 Jordan, R., 29, 30, 37 Kumar, A., 170, 179, 180
Hashem, N., 124 Jouneau, L., 65 Kumar, S., 179, 180
Hashiyada, Y., 77, 164 Jue, N. K., 109 Kurome, M., 202
Hassel, P., 122 Jung, J.-M., 78 Lacaze, S., 95
Havlicek, V., 131 Kalantari, A., 22 Lacerda, L. G., 134
Hazarika, J., 126 Kaneda, Y., 193 Lagutina, I., 35
Heng, S., 153 Kanengoni, A. T., 21 Lamas-Toranzo, I., 6
Hermosilla, C., 14 Kanuya, N. L., 20 Landeo, L., 99
Herrick, J. R., 73 Karnjanasirm, K., 19 Landschoot, G. V., 24, 199
Herrmann, D., 198 Karoon, S., 181 Lange, F., 199
Heywood, S., 173 Kaushik, R., 126 Lange-Consiglio, A., 149, 190
Hilari, F. Y., 70, 96, 100 Kelly, J. M., 47 Lanjewar, S. N., 205
Hill, J., 63 Kessler, B., 202 Lavagi, I., 108
Hinds, L. A., 93 Khan, I., 76 Lazzari, B., 190
Hinrichs, K., 51, 177 Kharzhau, A. A., 119 Lazzari, G., 35, 171
Hirako, M., 129 Khunmanee, S., 84 Lazzarotto, C. R., 58
Hirao, Y., 129 Kim, C.-L., 50 Leal, C. L. V., 172, 174
Hoffman, M. L., 109 Kim, D. H., 55 Lebedeva, I., 132
Holguin-Sanabria, G., 60 Kim, G. A., 27, 74, 91 Lebedeva, I. Y., 161
Holland, M. K., 63 Kim, H.-J., 204 Ledda, S., 47
Hong, S.-B., 34 Kim, H.-S., 204 Lee, B. C., 27, 33, 74, 91, 166
Huanca, W., 70, 96, 100 Kim, I.-S., 78 Lee, C.-I., 204
Huanca, W. F., 70, 96, 100 Kim, J.-W., 78 Lee, E., 163
Humblot, P., 113 Kim, K.-M., 197 Lee, H. T., 55, 56
Hwang, J.-Y., 76 Kim, M. G., 56 Lee, H.-., 191
Hyon, S.-H., 43 Kim, M. J., 33, 91 Lee, J.-., 191
Hyttel, P., 124 Kim, M.-S., 50 Lee, J.-H., 204
Hyun, S.-H., 163 Kim, N.-H., 72 Lee, J.-Y., 197
Ibraimova, N., 54 Kim, S. W., 50 Lee, K., 57, 152
Ille, N., 140 King, W. A., 175 Lee, K.-L., 75, 76
Imai, H., 192 Kitamura, Y., 192 Lee, S., 27, 74
Imai, K., 77, 135 Kjelland, M. E., 18, 130, 145, 146, Lee, S. H., 33, 91, 166
Imamura, S., 43 147 Lee, S.-J., 204
Iniesta-Cuerda, M., 144 Klee, R., 187 Lee, S.-L., 191
Isom, S. C., 173 Klinsrithong, S., 185 Lee, W.-S., 204
Ispada, J., 136 Klymiuk, N., 202 Leme, L., 101
Israel, S., 66 Köhne, M., 140 Leme, L. O., 69
Ivask, M., 36, 124 Kõks, S., 36 Leonardi, C. E., 62, 105
Jaakma, Ü., 36 Kong, I.-K., 38, 75, 76 Leonardi, C. E. P., 104
Jaca, J. I., 82 Kong, R., 75 Lewis, M. B., 207
Jang, G., 197, 204 Koo, D.-B., 78 Liang, S., 32, 157
Jegal, H.-G., 78 Koo, O.-J., 197 Liaudat, C., 203
Jemiller, E., 202 Kösters, M., 67 Licea, M. G., 130
Jeon, I. S., 50 Kotarski, F., 14 Lignier, S., 113
Jeong, P.-S., 78 Kotova, T. O., 201 Lillico, W., 12

Lima, E. A., 169 Matsuda, H., 77, 164 Ngcobo, J. N., 17
Lima, M. A., 183 Mayrhofer, C., 131 Niasari-Naslaji, A., 22, 121
Lira, B., 70, 96 McCollum, M., 5 Nie, Z.-W., 157
Liu, Y., 23 McGowan, M., 63 Niemann, H., 122, 198
Llamas-Toranzo, I., 203 McLennan, H. J., 153 Niu, Y.-J., 72
Lo Turco, E. G., 137 Meirelles, F. V., 183 Nkadimeng, M., 16, 21
Loi, P., 45 Méndez-Calderón, C. E., 31 Nociti, R. P., 151
Lombardo, D. M., 80 Mennibaeva, E. R., 142 Nogueira, G. P., 128
Lonergan, P., 59, 67, 110, 115, 116, Mentz, D. A., 114 Nogueira, M., 168
139 Mercadante, V. R. G., 152 Nogueira, M. F. G., 128, 136, 137
Long, C. R., 124 Mercadé, M. E., 40 Nol, P., 5
Looney, C. R., 126 Mermillod, P., 90 Nõmm, M., 36
López, H., 15 Mesalam, A., 38, 75, 76 Novgorodova, I. P., 143
López-Béjar, M., 40 Miglino, M. A., 58 Nowak-Imialek, M., 122, 198
López-Fernández, C., 145 Miguel-Gonzales, M., 87 Ntallaris, T., 113
López-Valiente, S., 82 Milazzotto, M. P., 136 Nunes, G., 168
Lopukhov, A., 132 Millar, R. P., 97 O’Neill, R., 109
Lopukhov, A. V., 161 Milner, D. J., 178 Ogata, Y., 164
Lorenzo, M. S., 80 Minami, N., 192 Ogluska, M., 64
Lorenzo, P. L., 6, 148 Mingoti, G., 168 Oh, H. J., 33, 91
Lozano, D., 83 Miriuka, S., 37 Ohkubo, Y., 129
Lucas-Hahn, A., 122 Mityashova, O. S., 161 Ohtake, M., 77, 164
Luzzani, C., 37 Miyoshi, A., 193 Oliveira, A. S., 137
Macedo, G. G., 51 Moawad, A. R., 49, 127 Oliveira, M. E. F., 151, 194, 195
Macente, B. I., 151 Mogas, T., 40, 175 Olivera, R., 29, 30, 37
Machado, A. B., 11 Mohanty, A. K., 180 Ongaratto, F. L., 31, 60
Machado, L. S., 183 Moley, L., 173 Opsomer, G., 159, 167
Maciel, G. S., 151, 194 Molina, A., 150 Orlandi, R. E., 169
Maculan, R., 102, 138 Molina, R. S., 99 Ortega, J., 83
Maeng, G., 25 Monguillot, I., 83 Ortega, Y. D. L., 145, 147
Maioli, M. A., 128 Monteiro, P. L., 69 Ortiz, W., 2
Maitreejet, W., 19 Morato, F. M., 137 Otsuki, J., 103
Majarune, S., 19 Moreira, G. M., 102, 138 Owen, C. M., 79, 81, 206, 208
Makalowski, W., 66 Moretti, R., 7 Pagano, N., 160
Malakar, D., 170, 179, 180 Moriyoshi, M., 135 Palazzese, L., 45
Manzoni, E. F. M., 112 Moro, L., 37 Palma-Irizarry, M., 130
Mapeka, M. H., 16 Moro, L. N., 26 Palomino, J. M., 88, 133, 182
Maqhashu, A., 17, 20, 21 Morotti, F., 7 Pamonsupornvichit, S., 184, 185
Marchetti, I. A., 89 Motohashi, H., 103 Pan, X., 25
Marchioretto, P. V., 60 Mphaphathi, M. L., 16, 20, 21, 46, Panahi, F., 22
Maresca, S., 82 71 Papillier, P., 161
Marqui, F. N., 8, 9, 10 Munar, C., 82 Papp, S., 131
Martinez, I. Y. H., 13 Mussi, M., 149 Paradiso, I., 160
Martínez, A., 145 Nakatsuka, M., 103 Paris, D. B. B. P., 97, 141
Martín-Maestro, A., 144 Nascimento, P., 90 Paris, M. C. J., 97
Martins Jr., A., 8, 9, 10 Natan, Y., 47 Park, B.-W., 191
Maruri, A., 80 Nedambale, T. L., 16, 17, 20, 46, 71 Park, B.-Y., 75
Mastromonaco, G. F., 133 Negota, N. C., 46, 71 Park, H.-J., 78
Matabane, M. B., 21 Nephawe, K. A., 16, 17 Park, S., 207
Matheson, R. L., 173 Nethenzheni, L. P., 46, 71 Parker, A. J., 141
Mathew, D. J., 110 Netshirovha, T. R., 20, 21 Pärn, P., 36
Matoba, S., 129 Newell-Fugate, A. E., 94 Paschoal, D. M., 172, 174

Pascottini, O. B., 159, 167 Raungprim, T., 19 Saini, S., 170, 179, 180
Pasquariello, R., 73 Ravida, N., 92 Salama, A., 127
Passaro, C., 110 Razavi, K., 121 Salamone, D., 165, 199
Paula-Lopes, F. F., 154 Rebollar, P. G., 6, 148 Salamone, D. F., 24, 26, 82, 98
Payton, R. R., 125 Redel, B. K., 162 Salazar, M. D. P., 87
Pedersen, H. S., 23 Redondo, B. I., 147 Salerno, F., 123
Pellegrino, C. A. G., 134 Reed, S. A., 109 Sales, J. N. S., 13, 102, 117, 137,
Pellerin, J.-L., 90 Reese, S. T., 125 138, 169
Peña Jr., S. T., 141 Regouski, M., 28 Salgado, R. M., 177
Peralta, O., 182 Resende, H. L., 177 Salvetti, P., 59, 67
Peralta-Martínez, M. Á., 146 Reyer, H., 119 Sánchez, J. M., 110, 115
Perecin, F., 183 Rho, G.-J., 191 Sánchez-Ajofrín, I., 144
Pereira, A. M., 51 Rho, N.Y., 175 Sanchez-Rodriguez, A., 148
Pereyra-Bonnet, F., 196 Rhyan, J., 5 Sandra, O., 113
Pericuesta, E., 203 Ribeiro, A. L. A. P. L., 102, 138 Santos, A. P. C., 169
Peris-Frau, P., 144 Ribeiro, C. V., 102, 138 Santos, G., 13, 117
Perri, S. H. V., 8, 9, 10 Ribeiro, I. F., 194 Santos, V. J. C., 151
Perrini, C., 149, 190 Richard, C., 113 Saragusty, J., 45
Pérsico, J. M. R., 89 Riedel, E. O., 67 Sariya, L., 184, 185
Petersen, B., 122 Rizos, D., 115, 116 Sarnyai, Z., 97
Peugnet, P., 65 Roberts, R. M., 162 Sartori, R., 69
Peyrás, S. D., 150 Robles, M., 65 Satake, N., 63
Phakdeedindan, P., 184, 186 Rochford, M., 92 Sato, S., 135
Pichardo, J. E. H., 130 Rodger, J. C., 93 Savy, V., 199
Pieiri, N. C.G., 183 Rodrigues, J. L., 31, 58, 60, 114 Scarlet, D., 85, 140
Pillai, S. M., 109 Rodrigues, M. G. K., 194 Schefer, L., 172, 174
Pinto, T. L. C., 102, 138 Rodrigues, S. A. D., 169 Schild, E. I., 82
Pisitkun, T., 52 Rodrigues, T. A., 154 Schoolcraft, W. B., 73
Piumi, F., 65 Rodrigues, V. H. V., 58 Schwarz, K. L., 174
Pizzi, F., 190 Rodrigues de Freitas, C., 114 Secher, J. O., 124
Plachta, N., 209 Rodriguez, N., 203 Seisenbayeva, A., 39, 54
Pohler, K. G., 125 Rodríguez, J. L., 130 Sekizawa, H., 193
Polejaeva, I. A., 1, 28 Rodríguez-Alonso, B., 115, 116 Seneda, M. M., 7
Polkoff, K. M., 188, 189 Rodriguez-Alvarez, L., 165 Seolwana, F. L., 16
Pontes, T. T., 169 Rodriguez-Villamil, P., 60 Serdal, D., 2
Pradebon, E., 11 Rogberg, A., 82 Sermyagin, A. A., 119
Prather, R. S., 162 Rogers, K. D., 41 Serota, N. R., 71
Pronarkngver, R., 184, 185 Romanenkova, O. S., 119 Seshoka, M. M., 16, 20
Pryor, J. H., 124, 126 Romo, S., 18, 130 Sestelo, A., 98
Psathaki, O. E., 66 Rondina, D., 31 Setthawong, P., 186
Ptak, G. E., 64 Rosales, B. C., 130 Setthawonge, P., 184
Quintao, C. C. R., 169 Ross, P., 150 Setyawan, E. M. N., 33, 91, 166
Radefeld, K., 131 Rossetto, R., 31 Seyedasgari, F., 22
Raggio, S., 89 Rossi, G. F., 137 Sharma, V., 170, 179, 180
Raito, B., 20 Rubessa, M., 123, 155, 188, 189 Shedova, E., 132
Raliou, M., 113 Ruiz, J. A., 99 Shedova, E. N., 161
Ramirez-Garzon, O., 63 Rungarunlert, S., 184, 185 Shin, H. D., 56
Rammutla, T. L., 71 Rungruangsak, J., 52 Shin, S. T., 55, 56
Ramón, M., 144 Russell, J. R., 125 Sikeao, W., 181
Ramukhithi, F. V., 16, 17 Ryan, P. L., 207 Silva, A. W. B., 102, 138
Raphalalani, Z. C., 16, 20 Ryu, J., 57 Silva, C., 168
Rattanatabtimtong, S., 19 Sahare, A., 126 Silva, E. P., 60

Silva, J. R. V., 102, 138 Tarasevych, Y., 195 Volchkov, P. Y., 111, 200
Silva, L., 14 Taubert, A., 14 Volkova, L. A., 142
Silva, L. O., 169 Taweechaipaisankul, A., 27, 74 Volkova, N. A., 111, 142, 143, 200,
Silva-Santos, K. C., 7 Taylor, M. A., 208 201
Silveira, J. C., 117 Techakumphu, M., 84, 184, 186 Volkova, V. V., 119
Silveira, M. C., 60 Tello, M. F., 80 Wakai, T., 103
Simmet, K., 108, 202 Teplitz, G. M., 80 Walker, S. K., 47
Simoes, L. M. S., 13, 169 Terán, E., 150 Wang, H., 61
Singh, J., 4, 62, 104, 105 Thakur, A., 170, 179, 180 Wang, Z., 28
Singina, G., 132 Thandavhathu, M. R., 20 Wani, N. A., 34
Singina, G. N., 161 Tharasanit, T., 184, 186 Watanabe, O. Y., 137
Siqueira, L. G. B., 101, 134 Therrien, J., 183 Watanabe, Y. F., 137
Sirard, M.-A., 88, 136 Thomas, J., 173 Weisgerber, D., 123, 155
Smith, J. K., 125 Thompson, J. G., 153 Weisgerber, D. W., 178
Smith, L. C., 183 Thongphakdee, A., 181 West, G., 94
Soares, J. G., 137 Tian, X., 109 Wheeler, M., 123, 155
Soler, A. J., 144 Ticiani, E., 58 Wheeler, M. B., 178, 188, 189
Sollecito, N. V., 134 Ticona, M., 96 Wilhelm, B. R., 58
Son, M.-J., 75 Timlin, C. L., 152 Willard, S. T., 207
Song, S.-H., 38 Tiwari, P., 126 Wiltbank, M., 11
Sørensen, C. B., 23 Toishibekov, Y., 39, 54 Wimel, L., 65
Soria, C., 86 Toishybek, D., 39 Wimmers, K., 119
Sotacuro, C., 99 Tokumaru, M., 43 Witt, R. R., 93
Souza, D. G., 8, 9, 10 Tomgorova, E. K., 142, 200 Wolf, E., 59, 67, 108, 202
Spate, L. D., 162 Torres, C., 182 Womack, S. A., 178
Spencer, T. E., 110 Tribulo, A., 83 Wrenzycki, C., 14
Stansfield, D. B., 187 Tribulo, H., 83 Wulf, M., 140
Steadman, C. S., 207 Tribulo, P., 83 Xu, L., 38
Stella, A., 190 Tribulo, R., 83 Yagüe, A. P., 147
Stradaioli, G., 112 Tummaruk, P., 52 Yamada, M., 192
Strillacci, M. G., 112 Uchuari, M., 100 Yamaguchi, M., 15
Strøbech, L. B., 124 Uh, K., 57, 152 Yamanouchi, T., 77, 164
Stroud, T. K., 18 Ulbrich, S. E., 120 Yang, M., 1
Sugawara, M., 193 Uzbekova, S. V., 161 Yang, S. X., 3
Sugimura, S., 77 Vajta, G., 39 Yang, S.-G., 78
Sukparangsi, W., 181 Van den Berghe, F., 97 Yao, L., 73
Sullivan, E., 28 Van Soom, A., 159, 167 Yermekova, M., 39
Sutton-McDowall, M. L., 153 Van Wettere, A. J., 28 Yerzhan, T., 53
Suvá, M., 165 Vasconcelos, G. L., 102, 138 Yeste, M., 40
Suwimonteerabutr, J., 52, 84 Velasquez, A., 165 Ynsaurralde-Rivolta, A. E., 82, 165
Suyatno, 192 Vendrell, M., 40 Yoon, J. D., 163
Suzuki, Y., 66 Vergara, J., 29 Young, C., 92, 187
Swangchan-Uthai, T., 84 Vetokh, A. N., 143, 201 Youngs, C. R., 87
Taher, L., 66 Viafara, J. A. S., 102, 138 Yu, H., 131
Takayama, M., 15 Viana, J. H. M., 101, 134 Yuan, Y., 73, 162
Takayama, O., 103 Vicente, W. R. R., 151, 195 Yum, S.-Y., 197, 204
Takeda, K., 129 Vichera, D., 29 Zacchini, F., 64
Takeda, T. T., 42 Vichera, G., 29, 30, 37 Zakhartchenko, V., 108, 202
Tanpradit, N., 44 Vigneault, C., 88 Zawar, S., 126
Tanyapanyachon, P., 107, 118 Villanueva, J. C., 70, 96, 100 Zhang, K., 61
Tapia, C., 89 Vivanco-Mackie, H. W., 87 Zhang, M., 109
Taradajnic, T., 132 Vogl, C., 131 Zhang, S., 38

Zhou, W.-J., 32 200, 201 Zwiefehofer, E. M., 62
Zinn, S. A., 109 Zolini, A. M., 2 Zwiefelhofer, E. M., 3, 12
Zinovieva, N., 132 Zullo, G., 160
Zinovieva, N. A., 119, 142, 143, Zuñiga, M. E., 99

2018 Recipient of the IETS Distinguished Service Award
Henrik Callesen
Dr. Henrik Callesen is the IETS Distinguished Service Award recipi-
ent for 2018. Callesen received his DVM and PhD from the Royal
Veterinary and Agricultural University (KVL, now part of the
University of Copenhagen). He later earned a Doctor of Veterinary
Science degree from the same university for his studies on super-
ovulation, oocyte maturation, embryo quality assessment, and donor
evaluation in cattle. He held research positions at KVL and the
Danish Institute for Agricultural Science (Aarhus University), where
he now serves as a professor in the Department of Animal Science.
His research in cattle and pigs has centered around ovum develop-
ment, superovulation, in vitro embryo production and cloning, embryo
cryopreservation, and embryo transfer. He served on the board of the
Association for Embryo Technologies in Europe, receiving that orga-
nization’s Pioneer Award in 2016. He has been a long-standing active
member of IETS, where he served on the Affiliate Society and Audit
Committees and on the board as a governor and then treasurer for five
years, followed by a term as vice president and then president in 2012.
This award was established by the Board of Governors in 1991. The primary purpose of this award is
to provide recognition for those individuals who have provided outstanding leadership or service to the
International Embryo Technology Society or who have contributed in a significant manner to the achieve-
ment of the stated purposes of the society as expressed in Article II of the IETS by-laws.
Article II–Purposes
Section 1. The society shall further the science of embryo production, development, and transfer
by (1) promoting more effective research; (2) disseminating scientific and educational infor-
mation; (3) fostering high standards of education; (4) maintaining high standards of ethics; (5)
enhancing the international movement of animal genetics with embryos; (6) coordinating stan-
dardization of embryo handling and record procedures internationally; and (7) cooperating with
other organizations having similar objectives.

Special Events
Morulas’ Preconference Workshop
Assessment of Mammalian Embryo Quality: Invasive and Non-Invasive Techniques
Saturday, January 13
16:00–19:00
The Study
The featured talks include the following: Tips for morphological evaluation of oocytes and pre-implantation
embryos, George Seidel, Colorado State University, USA; Transcriptional and epigenetic profiles of bovine
embryos and their relationship with developmental capacity, Heiner Niemann, Friedrich Loeffler Institute,
Germany; and Non-invasive methods for assessing the quality of embryos, Jeremy Thompson, the University of
Adelaide, Australia.
Sponsored by CSIRO Publishing
(Registration required)
Morulas and Mentor Luncheon

Sunday, January 14
12:15–14:00
Valley II
One of the main goals of the Morulas is to provide trainees the opportunities to interact with leading
scientists of the IETS. The Morulas and Mentors luncheon is designed to give trainees a chance to sit down with
mentors in small groups to develop meaningful connections with these recognized leaders. Join a number of out-
standing mentors at this annual event and choose from one of six mentors that you would like to dine with. Our
confirmed mentors are Dr. Dimitrios Rizos, Dr. Peter Sutovsky, Dr. David Kenny, Dr. Justin John, Dr. Bianca
Gasparini, and Dr. John Bromfield.
(Ticket required)
Welcome Reception
Sunday, January 14
18:00–19:00
Ballroom I and Pre-function Foyer
Sponsored by Professional Embryo Transfer Supple Inc. (PETS)
A welcome reception will be held in Ballroom I and the Foyer of the Shangri-La Hotel from 18:00 to 19:00.
Meet the exhibitors and renew old friendships. Light hors d’oeuvres will be served with a cash bar.
Morulas Career Luncheon

Monday, January 15
12:00–13:30
Valley II
Sponsored by CSIRO Publishing
This year’s Career Luncheon will feature a talk by two speakers, who will share unique perspectives from their
own personal career paths. Hear from Dr. Anna Denicol from the University of California, Davis, and Dr. Alessio
Valenza from CEVA Animal Health, Libourne. This forum provides a chance for trainees to gain perspective out-
side of their current work environment and meet others with similar aspirations.
(Ticket required)
Practitioners’ Forum
Monday, January 15
16:00–18:00
Myanmar
Current and future status of reproductive technologies
DABES Forum
Monday, January 15
16:00–18:00
Ballrooms II and III
Imaging the molecular and cell dynamics that form the early mouse embryo
Nicolas Plachta, Institute of Molecular and Cell Biology, A*STAR, Singapore

Open Meeting of the Health and Safety Advisory Committee (HASAC)
Monday, January 15
18:30–19:30
Ballrooms II and III
Morulas’ Trainee Forum

Monday, January 15
18:30–19:30
The Study
All trainees are invited and encouraged to attend the Morulas Trainee Forum. The Board of Governors will be
updating the membership on activities and attending to business matters. In addition, we will welcome the new
Morulas president and discuss important events and opportunities for all trainees. This is a great time to get
involved and boost your international relations.
(Everyone is welcome)
Morulas’ Student Mixer

Monday, January 15
19:30–20:30
After business comes fun! Shortly after the Morulas Trainee Forum, everyone is invited to gather with friends
and drinks for a social event. Hosted by IETS, this annual event is a fun time for all trainees to relax and enjoy
the atmosphere. Take advantage of meeting new people and establish connections that last a lifetime. The mixer
will conveniently be located in the hotel.
(Registration and tickets are NOT required)
Closing Party
Tuesday, January 16
20:45–23:45
Grand Chaopraya Cruise
Come join us for the event of the week. The closing party will be a private dinner cruise aboard the Grand
Chaopraya. There will be a Thai classical welcome dance, entertainment, dancing, buffet, and bar. The cruise will
be 3 hours and will pick up at and return to the dock of the Shangri-La Hotel. Check-in for the cruise will begin
at 20:15; the boat will leave at 20:45 sharp and return at 23:45.
(Tickets are required for this event.)

IETS Foundation 2018 Early Career
Achievement Award (Scientist)
Kiho Lee
Kiho Lee graduated from Seoul National University with a bachelor's
degree in animal sciences. Then, he received his MS and PhD from
Purdue University and postdoc training from University of Missouri–
Columbia. While at University of Missouri, he generated the first
immune-deficient pigs that could support growth and proliferation of
human-induced pluripotent stem cells. He joined Virginia Tech in 2013
and is currently an assistant professor in the Department of Animal
and Poultry Sciences. Lee’s lab currently focuses on early embryonic
development using the pig as a model. Understanding mechanisms
underlying the dynamic changes that occur during embryonic devel-
opment can lead us to develop more efficient ways to generate and
manipulate embryos in vitro. One of his main research interests is to
identify the mechanism of epigenetic reprogramming by oocytes after
fertilization. Specifically, he is interested in how oocytes can modulate
the status of DNA methylation during early embryo development. His
group also focuses on developing optimal use of gene-editing systems
to introduce targeted modifications during embryogenesis in pigs and
cattle. Using this approach, his group has generated various types of genetically engineered pigs for agricul-
ture and medicine.
This award recognizes two active IETS members (one practitioner and one scientist) for their independent
contributions toward advancing the field of embryo transfer and its associated technologies.
The objective of offering this award is to foster participation of young practitioners and scientists involved
with our society and recognize their excellence at the annual meeting. Currently, the society offers recogni-
tion of undergraduate and graduate students in the form of the Undergraduate Poster Competition, Student
Research Competition, and Peter W. Farin Student Travel Scholarship Award. It also recognizes long-
time members, offering the Pioneer Award and Distinguished Service Award. This award (Early Career
Achievement Award) is intended to fill the void in recognition between students and long-term established
members.
Previous Recipients
Pablo J. Ross (Scientist) 2017
Todd Stroud (Practitioner) 2017

Session Speakers and Keynote Biographies
Byeong Chun Lee
Byeong Chun Lee obtained his PhD from the Seoul National University, South Korea, and
received postdoctoral training at the University of Minnesota. He also worked at the Tokyo
University of Japan, University of New Orleans, and Finch University of Health Science/Chicago
Medical School, United States. Now he is a professor of theriogenology and biotechnology at
Seoul National University. He produced the first cloned dog, Snuppy, using somatic cell nuclear
transfer; the first transgenic cloned dog, Ruppy; and the cloned dog in which green fluorescent
protein gene is conditionally expressed, Teton. The goal of Lee’s research is directed toward pro-
ducing and elucidating the characterization of the human disease model of large animal models in dogs and pigs.
He has received numerous honours, including the Excellent Papers prize from the Korean Federation of Science
and Technology Societies in 2004. His research project was awarded for excellent research outcome in 2010 and
2014.
Andrés Gambini
Andrés Gambini graduated in 2008 with honors from the University of Río Cuarto, Agronomy
and Veterinary School in Argentina. After a short private practice, he spent five years at the
University of Buenos Aires completing a PhD program and as a teacher in charge of assignments
in Animal Physiology Chair in the animal production department at Agronomy School. There, he
began his research on in vitro embryo production in horses, particularly studying new strategies
to improve equine cloning. He was part of the team that produced the first cloned horses in South
America, and their continued efforts have resulted in several scientific publications in prestigious
journals. In 2015, he started his postdoctoral study at the National Institute of Environmental Health Sciences in
North Carolina, United States, where he studied mechanisms controlling the embryonic genome activation in
mammals. In 2017 Gambini joined, as a scientist visitor, the Melbourne University staff in Australia, where he is
continuing his research in bovine and horse cloning, ICSI, and other reproductive biotechnologies. Currently, he
is also a researcher for the National Scientific and Technical Research Council in Argentina.
Ursula Eichenlaub-Ritter
Ursula Eichenlaub-Ritter has performed studies in basic science of meiosis and germ cell forma-
tion with a focus on development, quality, and health of germ cells, especially cytogenetics,
molecular genetics, and cell und developmental biology of mammalian oocytes. Her research also
comprises studies on the safety of ART, including vitrification for fertility preservation; the
oocyte’s epigenome, proteome, cell cycle regulation, and mitochondrial activities, in particular
the relevance of age, environment, and pathologic conditions; and in vitro growth and maturation
of follicles and oocytes on oocyte and embryo quality and developmental potential.
Jon M. Oatley
Jon Oatley is the director of the Center for Reproductive Biology and an associate professor in
the School of Molecular Biosciences at Washington State University. Oatley obtained his PhD
from Washington State University in 2004, was a postdoctoral fellow in the laboratory of Ralph
Brinster at the University of Pennsylvania, and began as an independent investigator in 2007. His
research focuses on deciphering the mechanisms that regulate formation of the germline stem cell
pool in mammalian testes during development and maintenance of the population in adulthood.
Because the actions of the germline stem cell pool provide the foundation for continual spermato-
genesis, Oatley’s research is related directly to understanding fundamental processes that underpin male fertility.
Another major interest is the development of advanced methodologies for improving food animal agriculture
through reproductive processes. Oatley’s lab is currently utilizing cutting-edge gene editing technologies to
devise tools for advancing genetic gain in livestock populations that will improve the efficiency by which an
expanding global population is fed in the coming decades. Oatley has authored more than 50 papers in top-tier
journals in the fields of reproductive and developmental biology.

John J. Bromfield
John Bromfield is an assistant professor of reproductive physiology at the University of Florida’s
Department of Animal Sciences. Bromfield obtained his PhD at the University of Adelaide’s
Department of Obstetrics and Gynaecology (now the Robinson Institute) before participating in
postdoctoral studies at the University of Kansas Medical Center, Swansea University, and the
University of Missouri. Bromfield specializes in the field of reproductive immunology.
Specifically, he is interested in the role the immune system plays in facilitating optimal, healthy
pregnancy outcomes. The Bromfield laboratory has two main focus areas: (1) the role of semen in
modulating early pregnancy success and (2) the impact of uterine infection on ovarian function. In both of these
programmatic themes, Bromfield utilizes various bovine, rodent, and human in vitro and in vivo models.
Peter Sutovsky
Peter Sutovsky is professor of animal science in the College of Agriculture, Food, and Natural
Resources, University of Missouri, and professor of Obstetrics, Gynecology, and Women’s
Health at the School of Medicine, University of Missouri Health System. Since the early 1990s,
Sutovsky has studied mammalian gametogenesis, fertilization, and pre-implantation embryonic
development with special emphasis on the gamete and zygotic ubiquitin-proteasome system
(UPS). He was the first to describe the role of UPS in the regulation of mitochondrial inheritance
and introduced a novel concept of extracellular UPS, which has been validated in reproduction
and outside the reproductive biology field. In particular, Peter demonstrated the importance of sperm borne pro-
teasomes for mammalian fertilization and the role of UPS in epididymal sperm quality control, a mechanism rel-
evant to male fertility evaluation in livestock, and the diagnosis and treatment of human male fertility. Peter’s
collaborative research on the biogenesis and post-fertilization processing of sperm head perinuclear theca influ-
enced the optimization and safeguarding of assisted reproductive technologies/therapies such as in vitro fertiliza-
tion and intracytoplasmic sperm injection. Other notable collaborations include the development of transgenic
pig model for the study of 26S proteasome and work on rodent model of human endometriosis. Recently, the
Sutovsky laboratory has also been focusing on biomarker-based flow cytometric semen analysis and semen
nanopurification aimed at improving conception rates in livestock artificial insemination. As of August 2017,
Peter published 170 peer reviewed articles and monograph chapters; he has also edited two books and three jour-
nal special issues. He holds 14 US and foreign patents, reflecting his involvement in technology development
and commercialization.
Tiziana A. L. Brevini
Tiziana A. L Brevini graduated in 1989. She spent three years at the Department of Molecular
Embryology, Cambridge (United Kingdom). She obtained a PhD in 1994 and then carried out
research programs at Monash University, Melbourne, at the University of Adelaide, Australia,
and at the University of Miami, United States. She presently serves as full professor of anatomy
and embryology at the University of Milan, Italy. She is director of the Diabetes Research
Institute–UniMi and Chair of the COST Action “CellFit—In vitro 3D total cell guidance and fit-
ness.” Brevini is author of more than 300 publications. Her main area of research focuses on the
understanding of cell differentiation and commitment, epigenetic control of cell fate, and pluripotency-related
networks in mammalian somatic cells and embryos. She is married and has one daughter. She is a keen skier, a
qualified sailor, and has a passion for navigation and meteorology.
Ramiro Alberio
Ramiro Alberio is an associate professor in developmental epigenetics at the School of
Biosciences, University of Nottingham, United Kingdom. He graduated as a veterinarian from La
Plata University (Argentina) and gained his PhD in Germany under the supervision of Eckhard
Wolf (University of Munich). He did his first postdoctoral training with Keith Campbell
(University of Nottingham, United Kingdom). Currently, his laboratory is interested in under-
standing how uncommitted cells in the early embryo respond to inducing signals and segregate
into the different cell lineages that make up a fetus. His laboratory has studied the gene networks
that determine the emergence of pluripotent cells during the formation of the bilaminar embryonic disc in large
mammals and established the cross-talk between embryonic and extraembryonic lineages during the preimplan-
tation period. He uses a combination of classical embryology and single cell transcriptomics to gain insight into
cell fate decisions in early embryogenesis. His laboratory has also contributed to elucidating the transcriptional
and epigenetic program of primordial germ cells, the precursors of sperm and egg, in humans and pigs, demon-
strating conserved developmental mechanisms.
John Roche
John Roche is principal scientist for animal science at DairyNZ and managing director and prin-
cipal consultant for Down to Earth Advice Ltd. He has also held science appointments with the
National Centre for Dairy Production Research at Moorepark in Ireland, the Department of
Primary Industries in Australia, and the University of Tasmania. Roche leads a team of 12 scien-
tists, developers, and post-graduate students and has published approximately 150 peer-reviewed
science journal articles and book chapters. He is a regular contributor at international science and
farming conferences and has been a section editor for Journal of Dairy Science since 2012.
Roche is one of the most recognized authorities on the nutrition of grazing dairy cows, with a keen focus on prof-
itability. During the last two decades, his animal science programme has focused primarily on transition cow
nutrition and the role of body condition score and energy balance in milk production, health, and reproduction.
His review “Body condition score and its association with dairy cow productivity, health, and welfare” was the
most cited article in the Physiology and Management section of Journal of Dairy Science in 2010–2011. Roche
has also been involved in numerous genotype × diet comparison studies, identifying physiological reasons for
differences in body condition score change and reproductive efficiency in different genetic strains of Holstein-
Friesian cows. His research work in intake regulation identified diurnal patterns in the endocrine profiles of graz-
ing dairy cows that explain observed behavioral responses to feeding. Roche also has extensive publications in
grazing management, with a particular focus on the responsiveness of temperate grasses to carbon depletion, and
is well known for his expertise in grazing farm systems, having published some of the seminal applied studies in
stocking rate and farm system profitability.
David Kenny
David Kenny is a principal research scientist in Ruminant Nutritional Physiology with Teagasc in
Ireland and holds an adjunct professorship at University College Dublin. He has 20 years of
research experience in the biological control of a range of traits that are economically important
to ruminant livestock production systems. His work is based on in-depth study and the applica-
tion of state-of-the-art physiological and molecular approaches to these complex, multidimen-
sional traits. He has led a number of large multipartner research projects and has supervised the
studies of 15 PhD and nine MSc students to completion as principal supervisor. His research has
resulted in the publication of 130 full-length internationally peer-reviewed scientific articles to date as well as
many industry-targeted technical reports. He is currently leading a large Science Foundation Ireland research
project examining the molecular physiology of puberty and semen quality of bulls.
Justin St. John

Justin St. John was awarded his PhD from the University of Birmingham in 1999. While in the
United Kingdom, he was funded by the Medical Research Council and was appointed professor
of reproductive biology at the University of Warwick (2007). Since 2009, he has been the director
of the Centre for Genetic Diseases at the Hudson Institute of Medical Research. His research
focuses on understanding how mitochondrial DNA is transmitted and replicated. Using a variety
of assisted reproductive technologies and embryonic stem cell models, he has described mito-
chondrial DNA replication events in oocytes, embryos, and undifferentiated and differentiating
embryonic stem cells and why they are important to developmental outcome. He has also demonstrated why
donor cell mitochondrial DNA is transmitted to embryos and offspring following nuclear transfer. Additionally,
he has shown how mitochondrial DNA copy number is regulated in a cell-specific manner by DNA methylation
of the nuclear-encoded mitochondrial DNA-specific polymerase and how mtDNA haplotypes influence chromo-
somal gene expression patterns. He is using these outcomes to develop mini-pig models of autologous mitochon-
drial supplementation to enhance developmental outcomes. He has published in The Lancet, Nature Chemical
Biology, Nature Cell Biology, Nucleic Acids Research, Stem Cells, Cell Death and Differentiation, Journal of
Cell Science, and Genetics. In 2013, he received the Society for Reproductive Biology’s Award for Excellence in
Reproductive Biology Research.

Exhibit Hall Layout

Exhibit Directory
Booth Listing by Number:
Booth number Company

1 .......................................... Agtech Inc.
4 .......................................... IVF Bioscience
5 and 9................................... Vetoquinol
8 .......................................... Professional Embryo Transfer Supply Inc.
11 .......................................... IVF Technologies
12 .......................................... ECM Echo Control Medical
13 .......................................... ART Lab Solutions
14 and 18............................... WTA Technologies LLC
16 .......................................... Draminski S.A.

Alphabetical Listing of Exhibitors
Agtech Inc. prestigious research centers and scientists throughout
Agtech Inc. is your source for the embryo transfer the world. As a result of our commitment to quality,
products you can count on for success. In all that we our production plans comply with the most advanced
do, from product development to assisting with your technologies, combining modernity with experience to
product needs, it is the drive to build on our custom- deliver superior products and excellent service to our
ers’ success that keeps us moving forward. customers.
We are driven to help our customers succeed, helping Owocowa 17
them continue to be leaders in the industry and support 10-860 Olsztyn
their goals. Poland
Phone: +48 89 527 11 30
We take great pride in our relationship with our cus- Fax: +48 89 527 84 44
tomers and in providing them with the products, sup- www.draminski.com
port, and attention to detail that they have come to rely Booth: 16
on.
Echo Control Medical
8801 Anderson Avenue The company ECM has been in the field of ultrasound
Manhattan, KS 66503-9612 scanning for more than 30 years.
United States
Phone: 800-367-4016 We design and produce a complete range of ultrasound
Fax: 785-776-4295 systems for reproduction diagnosis and ovary exam.
www.agtechinc.com
Booth: 1 Come and see the Exago as well as the Exapad, which
can be equipped with on OPU guide and deliver
ART Lab Solutions an outstanding image quality for oocyte retrieval
In vitro produced embryos (IVPE) is the preferred applications.
embryo technology for genetic gain in cattle. ART Lab
Solutions is primarily focused on providing products 126 Bd De La Republique
and product development to those delivering repro- F-16000 Angouleme
ductive technologies in cattle breeding industries. France
Services provided include complete product range Phone: +33 5 45 92 03 57
of IVPE media for cattle embryo production, basic http://www.ecmscan.com
to comprehensive embryology training courses, and Booth: 12
laboratory and cattle IVPE consultancy. With an inno-
vative and experienced team, we are committed to IMV Technologies
delivering technologies that create a positive impact IMV Technologies is a world leader in reproductive
on cattle breeding. We source our innovations through biotechnologies.
our own research academic environment, providing IMV Technologies designs and develops equipment,
a means of translating research into industry sought- disposable items, and preservation media used in
after innovation. animal reproduction. Our areas of expertise include
the following:
ThincLab, 10 Pulteney Street • Embryo transfer
University of Adelaide • Semen collection and analysis
South Australia 5005 • Sample preparation and dilution
Australia • Packaging and cryopreservation
www.artlabsolutions.com • Assisted insemination
Booth: 13 IMV Technologies offers a wide range of ET products,
including collection and freezing media, filtration
Dramiński S.A. devices, laboratory equipment, embryo packaging, and
Professional equipment for veterinary and human transfer tools.
medicine, agriculture, and livestock breeders. Since
1987, Dramiński has been manufacturing specialized Our complete range can be found at www.imv-tech-
equipment designed for veterinary and human medi- nologies.com.
cine, livestock breeders, and agriculture.
IMV Technologies
We sell our products on all continents with the help ZI n 1
of 60 certified distributors. Our main areas of exper- 61300 L’Aigle
tise are portable diagnostic imaging ultrasound scan- France
ners and ovum pick-up solutions.We cooperate with +33 (0) 233 34 64 64

Email: [email protected] divided between livestock and companion animals
www.imv-technologies.com and includes most therapeutic categories. Vetoquinol
Booth: 11 embraces the challenge of finding better ways to help
animals and is committed to servicing the assisted
IVF Bioscience reproduction industry with its long-lasting tradition
IVF Bioscience manufactures high-quality, species- of excellence. The company boasts one of the largest
specific media for in vitro fertilisation (IVF). research facilities in the world, where 100 world-class
Our innovative suite of ready-to-use media is helping researchers passionately work to develop new prod-
many customers around the world to achieve higher ucts and protocols.
blastocyst rates and superior results.Our advanced,
serum-free media system is provided in combination Vetoquinol SA
with an optimised IVF protocol and backed by con- BP 189
tinuous technical support, so you can be confident that 70200 Lure
you are in good hands. We work with you to ensure Cedex
you get the best results possible. France
www.vetoquinol.com
With IVF Bioscience as your partner, establishing an Booth: 5 and 9
IVF laboratory has never been easier.
WTA Technologies LLC
Bickland Industrial Park WTA Technologies LLC is a Brazilian technology
Falmouth, Cornwall TR11 4TA company with additional offices in Texas. We special-
United Kingdom ize in producing products for animal assisted repro-
Phone: +44 1326 370642 duction, offering high added value solutions for ovum
http://www.ivfbioscience.com pick-up, in vitro fertilization, embryo transfer, and
Booth: 4 artificial insemination.
Professional Embryo Transfer Supply Inc. Our products are mainly for reproduction of cattle,
(PETS) horses, and small ruminants, but we also serve differ-
PETS has been a world-leading embryo transfer ent laboratorial requirements.
supply company in the bovine and equine industries
for three decades. Our goal all this time has been WTA sells throughout Brazil, the United States, and
your success, and we work every day to achieve this Mexico as well as in many countries across five con-
with quality service and ET supplies from ICPbio, tinents and is recognized as one of the leading compa-
Vetoquinol, MAI, SPI, Wesco, NovaVive, and more. nies in the market.
Come visit with us for more details.
Each product is designed to provide the very best
285 FM 16 result and give a sense of security at an economical
Canton, TX 75103 price while always being mindful of animal health.
United States Every piece incorporates precise design, quality mate-
www.pets-inc.com rials, and fine workmanship.
Booth: 8
WTA Brazil: + 55 16 3951 8161
Sales USA: + (979) 324-6168
Vetoquinol SA
http://www.wtavet.com.br
Vetoquinol, the manufacturer of Folltropin®, is a
Booth: 14 and 18
family-owned, independent company devoted exclu-
sively to animal health. Our product portfolio is

Thank You to Our Exhibitors

Morulas Preconference Workshop: Assessment of Mammalian Embryo Quality:
Invasive andNon-Invasive Techniques

CANDES Preconference Symposium
Monitoring and Controlling Ovarian Activity in CANDES
January 13, 2018

Program chair: Pierre Comizzoli
08:45–09:15 Registration, welcome, and introductory remarks

09:15 Keynote lecture
Comparative ovarian function and reproductive monitoring of endangered mammals
Janine Brown, Smithsonian Conservation Biology Institute, USA
10:00 Monitoring and controlling ovarian activity in wild felids
Ampika Thongphakdee, Zoological Park Organization of Thailand, Thailand
10:30 Coffee break
10:45 Monitoring and controlling ovarian activity in elephants
Chachote Thitaram, Chiang Mai University, Thailand
11:15 Monitoring and controlling follicular activity in camelids
Ahmed Tibary, Washington State University, USA
11:45 CANDES Trainee Travel Award
Femke Van Den Berghe, James Cook University, Australia
12:05 Lunch break
13:15 Monitoring and controlling ovarian activity in ungulates
Sadanand Sontakke, Centre for Cellular and Molecular Biology, India
13:45 Recent advances in tools and technologies for monitoring and controlling ovarian activity in
marsupials
John Rodger, University of Newcastle, Australia
14:15 Monitoring and controlling ovarian function in the rhinoceros
Terri Roth, CREW/Cincinnati Zoo, USA
14:45 Ovarian control and monitoring amphibians
Natalia Calatayud, San Diego Zoo Institute for Conservation Research, USA
15:15 Final discussion and remarks; coffee break
15:45 End of program

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44th Annual Conference

Shangri-La Hotel • Bangkok, Thailand • January 13–16, 2018

Building an Embryo: A Fertile Journey

Scientific Program Co-Chairs:

2017 IETS Board of Governors

Fulvio Gandolfi, President

Daniel F. Salamone, Vice President

Barbara S. Durrant, Treasurer

Marcelo Bertolini, Governor

Katrin Hinrichs, Governor

Irina A. Polejaeva, Governor

Dimitrios Rizos, Governor

Pablo J. Ross, Governor

International Embryo Technology Society 1

2 44th Annual Conference

Marc-André Sirard David Thomas Armstrong

Award Presentation: Tuesday, January 16, at 14:15

H. Niemann (2017) S. Willadsen (2005) R. L. Brinster (1992)

C. E. Pope (2016) B. Brackett (2004) A. K. Tarkowski (1991)

K. H. S. Campbell (2015) K. Betteridge (2003) J. D. Biggers (1990)

J.-P. Renard (2015) R. H. Foote (2002) C. Thibault (1989)

W. W. Thatcher (2014) P. J. Dziuk (2001) A. L. McLaren and D. Michie

J. Hahn (2013) R. Yanagimachi (2000) (1988)

O. J. Ginther (2012) R. M. Moor (1999) E. J. C. Polge (1987)

I. Wilmut (2011) I. Gordon (1998) T. M. Sugie (1986)

R. J. Mapletoft (2010) S. Wintenberger-Torres (1997) L. E. A. Rowson (1985)

S. P. Leibo (2009) W. K. Whitten (1996) L. E. Casida (1984)

G. Seidel Jr. (2008) C. R. Austin (1995) M. C. Chang (1983)

A. Iritani (2007) N. W. Moore (1994) R. O. Berry (1982)

D. Kraemer (2006) R. G. Edwards (1993)

International Embryo Technology Society 3

Shangri-La Wing (Lobby Level)

Shangri-La Wing (Level 1)

4 44th Annual Conference

Krungthep Wing (Level 3)

International Embryo Technology Society 5

Meeting Room Directory

Registration Desk Hours

Pick up of preregistration packets

On-site registration hours

Certificates of Attendance and Presentation

6 44th Annual Conference

Buying Thai Baht Outside Thailand

Exchanging Cash in Thailand is Easy

International Embryo Technology Society 7

Services and Amenities

DELIVERING THE BEST

8 44th Annual Conference

Session I: Clones and Lives Less Ordinary (Ballrooms II and III)

IETS Foundation Student Competition Presentations (Ballrooms II and III)

Session II: Ever-Decreasing Generation Intervals (Ballrooms II and III)

Selected Short Presentations (Ballrooms II and III)

10 44th Annual Conference

Selected Short Presentations (Ballrooms II and III)

International Embryo Technology Society 11

Selected Short Presentations (Ballrooms II and III)

Session V: Survival of the Fittest Embryo (Ballrooms II and III)

Selected Short Presentations (Ballrooms II and III)

Session VI: Keynote Lecture (Ballrooms II and III)