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    Kovaco Indeksu Formule

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    Milana Augustauskaitė
    The document provides information about a retention index standard product for gas chromatography. It consists of a mixture of aliphatic hydrocarbons from C8 to C32 dissolved in hexane. Components with carbon numbers that are multiples of five are at double concentration to allow easy identification. The standard can be used to calculate Kovats-type retention indices to aid in identification and method development. Typical temperature programmed chromatograms are provided as examples.

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    Kovaco Indeksu Formule

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    Milana Augustauskaitė
    62 views4 pages
    The document provides information about a retention index standard product for gas chromatography. It consists of a mixture of aliphatic hydrocarbons from C8 to C32 dissolved in hexane. Components with carbon numbers that are multiples of five are at double concentration to allow easy identification. The standard can be used to calculate Kovats-type retention indices to aid in identification and method development. Typical temperature programmed chromatograms are provided as examples.

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    Kovaco Indeksu formule

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    The document provides information about a retention index standard product for gas chromatography. It consists of a mixture of aliphatic hydrocarbons from C8 to C32 dissolved in hexane. Components with carbon numbers that are multiples of five are at double concentration to allow easy identification. The standard can be used to calculate Kovats-type retention indices to aid in identification and method development. Typical temperature programmed chromatograms are provided as examples.

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    © All Rights Reserved
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    Download as doc, pdf, or txt
    62 views4 pages

    Kovaco Indeksu Formule

    Uploaded by

    Milana Augustauskaitė
    The document provides information about a retention index standard product for gas chromatography. It consists of a mixture of aliphatic hydrocarbons from C8 to C32 dissolved in hexane. Components with carbon numbers that are multiples of five are at double concentration to allow easy identification. The standard can be used to calculate Kovats-type retention indices to aid in identification and method development. Typical temperature programmed chromatograms are provided as examples.

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    Product lnformation

    RETENTION INDEX STANDARD


    For Gas Chromatography
    SIGMA TECHNICAL BULLETIN #R8769
    Product No. R 8769

    Description:
    Sigma Retention Index Standard consists of a mixture of aliphatic hydrocarbons ranging from C8 through
    C32, dissolved in hexane. It is designed to be used to obtain Kovats-type gas Chromatographic retention
    indices, which are useful for preliminary identification of unknowns and as an aid in GC method development.
    Components with carbon numbers that are a multiple of five are at 2X concentration to allow easy
    determination of carbon numbers for peaks of interest.
    Composition:
    All components used are 98+% pure and are dissolved in GC grade Hexane at the nominal concentrations
    listed below:

    Component ug/mL Component ug/mL Component ug/mL


    n-Octane (C8) 1000 n-Hexadecane (C16) 1000 n-Tetracosane (C24) 1000
    n-Nonane (C9) 1000 n-Heptadecane (C17) 1000 n-Pentacosane (C25) 2000
    n-Decane(C 10) 2000 n-Octadecane (C18) 1000 n-Hexacosane (C26) 1000
    n-Undecane (C11) 1000 n-Nonadecane (C19) 1000 n-Heptacosane (C27) 1000
    n-Dodecane (C12) 1000 n-Eicosane (C20) 2000 n-Octacosane (C28) 1000
    n- Tridecane (C13) 1000 n - Honoicosane (C21) 1000 n-Nonacosane (C29) 1000
    n-Tetradecane (C14) 1000 n-Docosane (C22) 1000 n-Triacontane (C30) 2000
    n-Pentadecane (C15) 2000 n-Tricosane (C23) 1000 n-Dotriacontane (C32) 1000

    Typical Temperature Programmed Chromatogram


    Column 15m x 0.20mm x 0.2 um Supelco SPB-1 (Cat. No. 2-4162)
    Oven Temperature 30°C (0 min.), then 5°/min. to 300°C
    Calculations:
    A retention index value may be calculated for a peak by comparing its retention characteristics to those of the two closest
    eluting components in the RETENTION INDEX STANDARD, analyzed under identical conditions, using equations such as
    those found below.1-2 Presumptive identifications can often be made by comparing the Retention Index value to a value
    previously determined by you or values published in various literature references.3-7

    where: / = retention index for isothermal GC analysis


    IT = retention index for temperature programmed GC analysis, constant heating rate
    t'Ri = adjusted retention time of sample peak*
    t'Rz = adjusted retention time of n-alkane peak eluting immediately before sample peak*
    t'R(z+1) = adjusted retention time of n-alkane peak eluting immediately after sample peak*
    z = carbon number of n-alkane peak eluting immediately before sample peak
    tTRi = retention time of sample peak
    tTRz = retention time of n-alkane peak eluting immediately before sample peak
    tTR(z+1) = retention time of n-alkane peak eluting immediately after sample peak
    *Note: adjusted retention time = peak retention time minus retention time of an unretained peak
    Examples:
    Isothermal analysis
    Sample peak = 2.55 min. Unretained peak (air, methane, etc.) = 0.70 min. C18
    peak = 2.16 min. C19 peak = 2.81 min.
    Temperature programmed analysis

    Sample peak = 12.60 min. C18 peak=


    12.25 min. References:
    1) Basic Relationships of Gas Chromatography, Advanstar, Cleveland, 1993 R8769\4\8\2002
    2) Journal of Chromatography A, 657 (1993) 1-15
    3) Journal of Chromatographic Science, Vol. 19, May, 1981, 219-226
    4) The Sadtler Standard Gas Chromatography Retention Index Library, Sadtler Laboratories, Philadelphia, 1984
    5) Journal of Chromatography, 113 (1975) 69-95
    6) Instrumental Data for Drug Analysis, 2nd ed., Vol. 1-5, Elsevier Science Publishing, New York
    7) Clarke's Isolation and Identification of Drugs, 2nd ed., The Pharmaceutical Press, London, 1986
    For R&D use only. Not for drug, household or other uses. Sigma brand products are sold through Sigma-Aldrich, Inc.
    Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications.
    Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply.
    Please see reverse side of the invoice or packing slip.

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