C101-E094B

U V-1 700
PharmaSpec

Shimadzu
UV-VIS Spectrophotometer
 World-leading1nm
World-leading1nm resolution
resolution is
is
the
the best
best in
in its
its class
class

U V-17 0 0
P h a r m a S p e c
■ Specifications (wavelength accuracy, resolution, photometric accuracy,
etc.) and functions conform to the European, US, and Japanese
pharmacopoeias.
■ Hardware validation (9 items) as standard.
■ Supports IQ/OQ *
■ Compact design -
17 kg lightweight model can be easily moved by a single person.
■ Simple operation with specialized function keys.
Ease of operation via clear interactive LCD display.
■ Comprehensive functions for quantitative and qualitative analysis
installed as standard can be extended using IC cards.
PC-compatibility using latest Windows software.
■ Multi wavelength photometricmeasurement mode is standard.

* IQ/OQ contains the following details that are checked and recorded at the time
the equipment is installed. They are extremely important documents for
compliance with FDA guidelines.

1.IQ (Installation Qualification)

Confirms that the instrument has been properly installed in the original
configuration and in an appropriate environment.
2.OQ (Operational Qualification)
Confirms that the instrument has achieved the original target performance.

2
 Benefits
Benefits of
of 1nm
1nm resolution
resolution

Substances containing benzene rings exhibit sharp The UV-1700 comfortably clears the wavelength
absorption bands near 250 nm. In such cases, a 1 nm resolution standard prescribed in the European
difference in resolution is clearly apparent. The diagram Pharmacopoeia. The European Pharmacopoeia demands
above shows the spectra of an ethanol solution of a ratio exceeding 1.5 between the peak value near 269
benzene obtained with the UV-1700 and a previous nm and valley value near 266 nm for a toluene solution in
instrument with a 2 nm resolution (bandwidth). The hexane. This ratio is 2.23 in the diagram above obtained
diagram confirms a maximum 40% difference due to the with the UV-1700. (Diagram displayed using the
different resolutions. (Diagram displayed using the UVProbe PC software.)
UVProbe PC software.)

Wavelength(nm)

The wavelength accuracy check is conducted using optical wavelength accuracy check filters.
(The diagram shows the spectrum and peak-detection results for an NIST standard holomium
solution SRM2034 for wavelength calibration.)

3
 UV-1700 PharmaSpec

From photometric to multi-component quantitation,

almost all of the functions required in the lab are built in.
Spectrum
A samples spectrum is recorded using
wavelength scanning. Repeat scans let you
follow sample changes over time. Zoom in on the
finished spectrum for a better view, and then use
the peak/valley pick function to select maxima
amd minima, and perform a wide variety of data Quantitation
processing functions. Generates a calibration curve from measurement
of standards, and then calculates the
concentrations of unknowns. Allows various
combinations of wavelength number (1 to 3
Kinetics wavelengths, peak area values) and calibration
Measure absorbance changes as a function of curves (K factor, 1st to 3rd order).
time, and obtain the enzymatic activity value.
Select among single wavelength, multicell,
double wavelength and rate measurement
methods. Using the MMC-1600/1600C (8/16
cells) or the CPS-240A Cell Positioner (6 cells),
multiple samples can be measured at the same
time.
Time Course Scan
Measures the changes in absorbance,
transmittance or energy as a function of time.
Using the MMC-1600/1600C (8/16 cells) or the
CPS-240A Cell Positioner (6 cells), multiple
samples can be measured at the same time.

Multi-Component Quantitation
Quantitates up to 8 components mixed in a single
sample. The calibration equation is determined
using pure or mixed components with known
values.

Optional Application Programs

In seconds, the easy-to-insert program packs
Multi Wavelength Measurement reconfigure the UV-1601 for specific laboratory
Measure the absorbance and transmittance at protocols.
up to eight designated wavelengths. For
absorbance measurements, calculation of data
for up to four wavelengths, including the
difference or ratio between two wavelengths, is
possible. Instrument Conditions
The basic instrument conditions can be set to
accommodate the method of use for even greater
convenience. And, built-in self-performance
check functions ensure the continued reliability of
measurement data.

4
Wavelength repeatability and other automatic test
functions are built-in, providing easy compliance with
GLP and ISO-9000 requirements.
Photometrics PC Control
Measures absorbance or transmittance at a The built-in RS-232C allows connection between
wavelength of your choice. By connecting an the UV-1700 and your PC, permitting full control
optional multicell sample compartment, up 16 from the PC, automatic system configuration,
samples can be measured continuously (when data exchange, and an expanded array of data
using MMC-1600/1600C and 16-cell micro processing functions.
multicell). And, data may be conveniently stored
on IC cards.

Maintenance
The maintenance functions include an indication
of the date and time of the previous baseline
correction, confirmation of lamp usage time, and
unit validation.

IC Cards
Special IC cards greatly extend the functionality
of the UV-1700 system. IC card utilities allow
Parameter Recall data pack copying, IC card initialization, and
The measurement mode and its parameters can other functions.
be stored in memory or on IC cards, available for
recall and setting at any time. Operation is easy
and there is less possibility of making setting
errors.

5
 UV-1700 PharmaSpec

From transparent samples to samples with suspended and

components, they can all be measured and quantitated. Even
volume and enzyme activity measurements are possible.
1. Micro Volume Sample Measurement
3uL capillary cells and 50µL ultramicro cells are available
for measurement of minute volumes of sample. The spectra
in the first figure were obtained from measurement of DNA
in herring sperm in phosphate buffer solution. (A 3µr
capillary cell was used.) Even though measurement was
conducted in the ultraviolet region, the light beam was
condensed sufficiently to provide a spectrum with a high
signal-to-noise ratio. The second figure shows the linearity
of the calibration curve.

2. Multicell Kinetic Measurement

Multicell kinetics measurement can be performed using a
temperature controlled cell positioner. At right, the enzyme
activity of cholinesterase in blood serum was measured.
Enzyme reactions can be measured in up to 16 cells at one
time.

6
interfering
micro

3. Multi Wavelength Measurement

Measures the absorbance or transmittance for a sample at
up to eight selected wavelengths. When absorbance is
measured, results can be displayed or output by calculating
data using up to four wavelengths.
Note : If a printer is connected, results are printed after each measurement.
(The data cannot be saved for individual measurements.)

4. Multi-component Analysis
Quantitative analysis can be performed for up to 8
components mixed in a single sample. At right, aspirin,
caffeine and salicylic acid were present in a sample in ratios
of 2 : 1 : 1, as confirmed in the separated quantitation
result.
The second figure shows the spectral characteristics of the
three ingredients and the mixture. Note the distinctly
different spectral characteristics, allowing the various
compounds to be distinguished.

Mixture sample

Salicylic acid Caffeine

Aspirin

7
 UV-1700 PharmaSpec

5. Instrument Validation Functions

These functions can conduct performance checks
(measurement, pass/fail evaluation and printing) for nine
items, including wavelength accuracy.
Validation is divided into fully automatic checks, where all
procedures from measurement to evaluation are automatically
completed, and semi-automatic checks, where the insertion
and removal of inspection jigs are required.

The semi-automatic checks are performed interactively. The

user can conduct the validation by inserting or removing
inspection jigs as instructed by messages on the screen.

The control numbers and expiration date of the inspection jigs

can be entered in the inspection conditions. The expiration
date is automatically checked at the start of the test and the
validation is discontinued if a jig is past its expiration date.

8
 Printing Examples
Validation results can be printed out if a printer is connected. The two printed forms available are the detailed results form that
shows the spectrum and time-course data (curves) and results for each test, and the general results form that lists the results for all
selected validation items after they are complete.

<Detailed results form> <General Results form>

6. Maintenance
The lamp usage times for the deuterium (D2) and halogen
(WI) lamps are counted. The lamp usage time is displayed
during periodic maintenance to determine when the lamps
need to be replaced.

9
 UV-1700 PharmaSpec
Spectrophotometer Specifications (UV-1700)
Item Specification Item Specification
Spectral bandwidth 1 nm (190 to 900 nm) ±0.002 Abs (at 1.0 Abs)
Photometric repeatability
Dimensions 550W × 470D × 200 H (mm) (max. height 380 mm) ±0.001 Abs (at 0.5 Abs)
Weight 17 kg Baseline stability 0.001 Abs/h max (700 nm, After 1hour from power on)
Wavelength range 190.0 to 1100.0 nm Baseline flatness ±0.001 Abs (1100 mm to 200 mm) After 1hour from power on
Wavelength display 0.1 nm increments Noise level 0.002 Abs (P-P) 0.0002 A (RMS) (700nm)
0.1 nm increments (1 nm increments when Automatic with computer memory, in two
Wavelength setting Baseline correction
setting scan range) stages of coarse and fine
Wavelength accuracy ±0.3 nm 20 W halogen lamp, deuterium lamp
Light source
Wavelength repeatability ±0.1 nm Built-in light source automatic position adjustment
Wavelength slew speed: Approx. 6000 nm/min Monochromator Aberration-corrected blazed holographic grating
Wavelength Scan Rates Wavelength scanning speed: Approx. 3000 Detector Silicone photodiode
nm/min to 10 nm/min Internal dimensions: 110.0W × 230.0D × 105.0H (mm)
Lamp interchange wavelength 295.0 nm to 364.0 nm (340.8 nm) Distance between light beams: 100.00 mm
Sample compartment
Stray light 0.04% max. (220.0 nm NaI, 340.0 nm NaNO2) Installation: Fixed with two screws
Photometric system Double-beam Optics Beam size: 9 x 0.5 mm
Absorbance: -0.5 to +3.0 Abs 100, 120, 220, 240 VAC
Photometric range Power requirements
Transmittance: 0.0 to 300% 50/60 Hz, 130 VA
Absorbance: -3.99 to +3.99 Abs Ambient requirements Temperature: 15 to 35˚C, humidity: 35 to 80%
Recording range
Transmittance: -399 to +399%
±0.004 Abs (at 1.0 Abs), using NIST930D filter
Photometric accuracy
±0.002 Abs (at 0.5 Abs)

Overall Dimensions (UV-1700)

550
Max380
470

200

470
Units: mm

10
UV-1700 Software Specifications
Measurement mode Specifications Measurement mode Specifications
1 Measurement at user-selected fixed wavelength 1 Measurement mode: Abs, T%, E
Photometric 2 Quantitation using K-factor method Measurement time: 1 to 6500 sec/min
3 Photometric modes: Choice of T% and Abs 2 Supports Cell Positioner CPS-240A, 6-cell Multicell
Mode
4 Data table storage and recall functions Time Scan Sample Compartment, MMC-1600/1600C and Sippers
5 Automatic data printout 3 Measures changes in Abs, T%, or energy over time
4 Data processing: Identical to Spectrum Mode
1 Measurement modes: Abs, T%, E
5 Data export via RS-232C port
Scanning range: 190.0 to 1100.0 nm
Ordinate range: Abs : -3.99 to +3.99 Abs 1 Up to eight components quantified at once.
T%, E : -399 to +399% 2 A mixture, as well as pure components, can be used as
Scanning speed: Very Fast, Fast, Medium, Slow, and Multi-Component a standard.
Very Slow Quantitation 3 Data on standards can be stored, in addition to
Number of repeat scans: 1 to 99 measurement wavelengths.
Recording system: Selection between single spectrum 4 Quantitation of recalled spectrum data.
and data overlay
1 Measurements at up to eight designated wavelengths (set
2 Data processing
Spectrum in 0.1 nm increments)
Peak/valley detection (up to 20 of each)
2 Data calculation at up to four wavelengths (difference
Mode Area calculation Multi Wavelength or ratio between two wavelengths, calculation between
Arithmetic operations between data
Measurement three wavelength, etc.) possible.
Arithmetic operations between data and constants
3 Photometric mode: Abs, T%
1st to 4th derivative/smoothing
4 Results can be printed after each measurement. (The data
Point pick
cannot be saved for Individual measurements.)
Data storage and recall
(up to 6 in main memory, 27 in data pack) 1 Baseline correction
Data output 2 Lamp usage time display and reset
Graph axis range change 3 Instrument validation functions
Data readout at cursor-specified point 1) Compatible with 9 JIS items
3 Data export via RS-232C port Wavelength accuracy, wavelength repeatability,
resolution, stray light, photometric accuracy, photometric
1 Measurement methods
repeatability, baseline flatness, baseline stability, noise
Choice of 1-wavelength, 2-wavelength, 3-wavelength,
level, initialization result recording
1st to 4th derivative methods
2) Semi-automatic validation
2 Quantitation methods
Validation inspections conducted interactively while
Automatic concentration calculation by K-factor Maintenance
inserting and removing inspection jigs.
Automatic concentration calculation using single-point
3) Fully automatic validation
calibration curve or multi-point calibration curve
Automatic validation inspections from measurement to
Quantitation method (1st to 3rd order regression curves)
evaluation and printout.
3 Measurement parameters
Mode 4) Setting inspection parameters and pass/fail criteria
Number of repeat measurements: 1 to 9, to obtain a mean
Authority to make changes can be protected by password access.
value for quantitation
5) Bulk printout of results
Order of derivatives: 1st to 4th
Results of the previous validation inspection are printed
Number of standards for multi-point calibration curve: 2 to 10
in list form. (The results of the previous inspection are
Order of calibration curve: 1st to 3rd
cleared when another inspection is started.)
Choice of zero or non-zero intercept
4 Data table storage and recall 1 Automatic setting of measurment mode after instrument
5 Automatic data printout initialization
Designation of a parameter file is possible.
1 Measures absorbance changes as a function of time and
2 Backup of previous measurement parameters
calculates the enzymatic activity values.
3 Selection of displayed number of decimal places
Measurement time: 1 to 6500 sec/min
Absorbance: 3 or 4 decimal places
2 Measurement method: 1-wavelength, 2-wavelength,
Transmittance: 3 or 4 decimal places
multicell and rate measurements
Shared 4 ON/OFF of the beep function
Kinetics 3 Supports Cell Positioner CPS-240A, Multicell
5 Number of files that can be saved:
Holder and Sipper 160 Functions
Mode Measurement parameters: 16 files max.
4 Data processing
Data: 6 files max.
Recalculation
6 Screen hard copy printout (when a printer is connected)
Graph axis range change
Date stamping can be selected between ON and OFF.
Data table display
7 PC control
Data storage and recall
Spectrophotometer can be controlled by an external PC
5 Data export via RS-232C port
(via RS-2332C).
The optional UVProbe software controls this instrument.
11
 UV-1700 PharmaSpec

ACCESSORIES
UVProbe Software
(Cat. No. 206-89456-91)
UVProbe is software to control the UV-1700 from a PC. The
all-in-one software package includes spectrum, photometric
(quantitation), kinetics (time course), and report generator
functions.
An RS-232C cable is required to connect the UV-1700 to the PC.

Required and recommended PC configuration for running

UVProbe is as follows:
● PC with Pentium (200MHz) or better processor, or 100% compatible.

(Pentium II (300MHz) recommended)

● 64MB RAM, 128MB recommended

● 50MB available hard disk capacity

● SVGA video monitor, 800 × 600 dot screen resolution (1024 × 768

recommended)
● Parallel and serial ports

● Graphic printer, or plotter ( recommended for printing data)

● Mouse or similar pointing device

● CD-ROM drive

Even with the above configuration, UVProbe operating performance cannot be

guaranteed, depending on Windows settings, hardware state, etc.
Use Shimadzu recommended equipment, if possible.

Loaded with Data

Processing/Calculation Functions
Peak detection, Point Pick, area calculation, and many more-
UVProbe includes all the data processing functions you'll need for
spectrum and time course data. A variety of calculations are also
yours for the choosing, like integration and interpolation
conversions, and arithmetic operations between data.

GLP/GMP Compliant
For example, when data processing is performed, the resultant data is stored
together in the same file with the original data, which remains intact.
The audit trail function tracks the instrument history, and security functions,
such as the limiting of individual users to specific types of software
operations, ensuring full GLP/GMP compliance.

Powerful Quantitation Functions

The Photometrics module permits multi wavelength and
single wavelength quantitation, and quantitation based on
peaks as well as area values.
Calibration curves can be generated using K factor, single or
multi point methods.
Further, customized functions can be created to determine
pass/fail of measurement results.
12
Totally Customized Reports
Arrange graphs and data just as you wish to create informative, easy-to-
read reports. Freely specify line thickness and font color and size, and place
labels wherever you like on graphs.
Because the setup is all in WYSIWYG format, layouts can be stored as templates to enable fast and simple generation of future
reports.

Software Specifications
Operating System Windows NT4.0,Windows 95/98/2000 and other corrections using factors
Data Acquisition Spectrum, Kinetics (time course measurement) and ● Averaging of repeat measurement data
Modes Photometric (quantitation) ● Simultaneous display of standard table, unknown

● Multitasking (simultaneous measurement and data table and calibration curves

processing and other types of processing) ● Display of Pass/Fail indications

● Customizable measurement screen layout ● Comparison/relative processing of multiple time

General (wavelengths, data display font and font size, colors, course data∗2
displayed number of rows) ● Single or double wavelength measurement

● GLP/GMP compliant (security, history) (difference or ratio)

● Real time concentration display ● Simultaneous display of time course data, enzyme

● Comparison of multiple spectra/relative table and graphs

processing*2 ● Enzyme kinetics calculation (for single or multicell)

● Save all processed data with original data set Kinetics ● Michaelis-Menten calculations and graph creation

Spectrum Mode including a history of all manipulations (Time Course) (Michaelis-Menten, Lineweaver-Burk, Hanes,
● Spectrum enlargement/shrinking, auto scale and Mode Woolf, Eadie-Hofstee), Dixon plot, Hill plot
Undo/Redo of these operations ● Unitary management of sample information

● Annotation on spectrum screen including original data, sample weight and dilution
● Normalization, Point Pick, peak/valley detection, factors, etc.
area calculation ● Event recording such as addition of reagents during

● Transformations : 1st - 4th derivatives, smoothing, measurement

reciprocal, square root, natural ● Time course spectrum data processing (same as in

Data Processing log, log, Abs. to %T conversion, spectrum data processing)

in Spectrum Mode exponential, Kubelka-Munk ● Preview and print functions for customized formats

conversion ● Layout and editing of templates

● Ensemble averaging, interpolation, data set and ● Quick printing using report templates

constants arithmetic (between spectra, between ● Multi-page printout support

spectra and constants) ● Insert date, time, text, and drawing objects including

● Single wavelength, multi wavelength (includes 1, 2 Report Generator lines, circles and rectangles
or 3 wavelengths), spectrum quantitation (peak, ● Insert spectrum and quantitation data, method and

maximum, minimum, area, etc. for specified history

wavelength ranges) ● Headers and footers easily inserted
Photometric ● Multi-point, single point, K-factor calibration curves ● Specify graph line thickness (as in all modules), font
(Quantitation)
Mode (1st, 2nd, 3rd order function fits, pass-through-zero style and size
specification) 1) PC, monitor and printer are not included.
● Photometric processing with user-defined functions (+, 2) Depends on PC environment (memory, etc.). As a guideline, from 20 ~ 30 spectrum data
-, ×, ÷, Log, Exp, etc. functions, including factors) sets.
● Weight correction, dilution factor correction,

13
 UV-1700 PharmaSpec

ACCESSORIES
Time Course (Multi λ) Program Pack
(Cat. No. 206-80704-02)

Measures changes in absorbance or transmittance for up to 6

wavelengths, with respect to time. The changes are presented as time
course curves for the selected wavelengths.
● Number of wavelengths : 6 max. (wavelength adjustment in 0.1 nm steps)
● Photometric mode : Abs. or T%
● Repeated measurement : Up to 99 repetitions and up to 9999 seconds
measuring time
● Graphic ←
→ tabulated display changeover
● Automatic data printout
Parameter Selection

Display during Measurement

Data Pack
(Cat. No. 206-80700)

Stores operational parameters as well as data.

● Stores up to 79 sets of operational parameters per pack.
● Stores up to 27 data sets per pack.

∗ This Data Pack is identical to the UVmini-1240 Series Data Pack but the
files are not compatible.
The Data Pack must be initialized for use with the UV-1700.

14
Protein Quantitation Program Pack
(Cat. No. 206-82850-02)

Determines protein concentration in any of the four coloring

methods or directly from the absorbance at 280nm. The instrument
parameters for quantitation are incorporated.
● The following five quantitation methods are included.
• Lowry Method
• BCA Method (method using Bicinchoninic Acid)
• CBB Method (method using Coomassie Brilliant Blue G-250)
• Biuret method
• UV Absorption (direct measurement at 280nm)
● Features of methods other than UV Absorption, Quantitation Selection

• K-factor method-Values for K and B may be input for calculation using :

C = K × ABS + B
• In the single point calibration curve method, a single standard is measured
to generate a calibration curve, which passes through the origin.
• In the multi-point calibration curve method, up to 10 standards are
measured to generate either a linear calibration curve or a second order
calibration curve.
• Up to 9 repeat measurements can be conducted to obtain a mean value
for use in quantitation.
• Selection from any of four concentrations is possible: mg/mr, µg/mr, etc.
• The equation used for generating the calibration curve as well as any
determination coefficients may be displayed.

Parameter Selection (Lowry method)

DNA/Protein Quantitation Program Pack

(Cat. No. 206-82851-02)

Determines DNA or protein concentrations directly from the UV

absorption bands at 230nm, 280nm and 260nm. No coloring
procedure is required.
Quantitative data are obtained through simple keyboard operation,
using the resident formula. The wavelengths and factors used in the
computation are arbitrarily changeable.
● The following two formulas are user selectable:
1) Formula using absorbances at 260.0nm (A1) and 230.0nm (A2)
Absorbance ratio = A1/A2
DNA concentration = 49.1 × A1 - 3.48 × A2 Parameter Selection
Protein concentration = 183.0 × A2 - 75.8 × A1
2) Formula using absorbances at 260.0nm (A1) and 280.0nm (A2)
Absorbance ratio = A1/A2
DNA concentration = 62.9 × A1 - 36.0 × A2
Protein concentration = 1552 × A2 - 757.3 × A1
● The absorbance at 320nm can be used for background correction.

Measurement Display

15
 UV-1700 PharmaSpec

ACCESSORIES
DPU-414 Screen Copy Printer
(Cat. No. 206-55215-∗∗)

Prints hard copies of screens, including numeric data. A printout is

made after each measurement.
Spectra, kinetics reaction data, and quantitation calibration curves
displayed on the screen are output in the screen print. A hard copy
can be printed at any time, making it simple to record measurement
parameters.
Thermal paper (10 rolls): 088-58907-04
The printer cable is included with DPU-414.
Dimensions: 160 x 170 x 66.5 mm

Interface Cable (Centronics standard)

(Cat. No. 200-91536-15)

This cable connects the UV-1700 to any general commercial printer

(limited to ESC / P specifications) to allow printout. Color and
monochrome printers are both supported.

Note : For information regarding specific printer types, please contact

your Shimadzu representative.

Analog Output Interface

(Cat. No. 204-04757)

● Allows analog output for monitoring a liquid chromatograph, etc., and can
connect to an integrator.
● Analog output full scale
100mV / 2Abs or 100mV / 100%T

Tm Analysis Software
(Cat. No. 206-57476-91) RS-232C RS-232C
PC
● This software works with the S-1700 and accumulates temperature-versus-
absorbance curve data at the PC to analyze the Tm (melting temperature)
of nucleic acids such as DNA and RNA. The right figure is a typical setup
for this software. Spectrophotometer S-1700
● RS-232C cables (Cat. No. 200-86408) are needed to connect the PC to the
S-1700 and the spectrophotometer.

16
CPS-240A Cell Positioner,
Thermoelectrically Temperature Controlled
(Cat. No. 204-05837-∗∗)
This attachment permits measurement of up to six sample cells
under constant temperature conditions. Combination of this
attachment and the Kinetics mode provides measurement of
temperature sensitive enzyme kinetics of one to six samples.
● Number of cells : 6 on the sample side (temperature controlled)
1 on the reference side (temperature not controlled)
● Temperature control range : 16 to 60˚C
● Temperature display accuracy (difference from the true value) : ±0.5˚C
● Temperature control precision (variation of temperature) : ±0.1˚C
● Ambient temperature : 15 to 35˚C

Note : Sample cells (Cat. No. 200-34442) are not included in the
standard contents.

TCC-240A Thermoelectrically
Temperature Controlled Cell Holder
(Cat. No. 204-05557-∗∗)
Uses Peltier effect for controlling the temperatures of the sample and
reference sample. No thermostatic bath or cooling water is required,
so the operation is quite simple and easy.
● Number of cells : One each on the sample and reference sides
● Temperature control range : 7 to 60˚C
● Temperature display accuracy (difference from the true value) : ±0.5˚C
● Temperature control precision (variation of temperature) : ±0.1˚C

Note : Sample cells (Cat. No. 200-34442) are not included in the
standard contents.

S-1700 Thermoelectric Single Cell

Holder
(Cat. No. 206-56000-91)
This cell holder permits setting of a temperature program to increase
and decrease the sample cell temperature.
● The thermoelectric system allows prompt control of sample
temperature between 0°C and 110°C.
● Temperature increase/decrease speed can be changed using 12
settings, which means the holder can be used in analysis of
melting curves for nucleic acids, etc., that occur during quick as
well as slow heating (or cooling).
● A stirrer also is provided to ensure uniform temperature
distribution throughout the cell.
● A cooling water circulation is required for Peltier element cooling.
And though tap water can be used, it is recommended that a
commercially available constant-temperature water circulator be
used, as the following conditions must be fulfilled to exact
maximum performance from the S-1700.
· Cooling water specification: 20 ±2°C
· Water flow: 4.8L / min or more
● Temperature is not controlled at the reference side.
● Cells are not supplied. Please use 10mm square tight-sealing cells
(a Hellma product).
Type Optical Path Minimum Sample Volume Required
110-QS-10 10mm 3.5mL
115B-QS-10 10mm 400µL
● Temperature accuracy in cell (when room temperature is 25°C)
Within ±0.25°C (0 to 25°C)
Within ±1%°C of set value (25 to 75°C)
Within ±2%°C of set value (75 to 110°C) 17
 UV-1700 PharmaSpec

ACCESSORIES
Sipper Unit 160L (Standard Sipper)
(Cat. No. 204-08270-01)

Sipper Unit 160T (Triple Pass Sipper)

(Cat. No. 204-08270-02)

Sipper Unit 160C (Constant Temperature Sipper) Sipper L 2.0mr

Sipper T 1.5mr
(Cat. No. 204-08270-03)
Sipper C 2.5mr
Sipper U 0.5mr
Sipper Unit 160U (Supermicro Sipper)
(Cat. No. 204-08270-04)

The four sippers listed above are available, depending on the flow Note : The use of a Teflon Valve Unit (Cat.No. 204-06599-01) and
cell shape. A peristaltic pump driven by a stepping motor ensures the SWA-2 Sample Waste Unit (Cat.No. 204-29230) are
reliable and smooth aspiration of sample solution. (Direct drive is recommended when strong acids, strong alkalis, or organic
possible from the UV-1700, so no interface is required.) solvents are to be measured.

ASC-5 Auto Sample Changer

(Cat. No. 204-09100-∗∗)

Combine with a Sipper 160 to build an automated multisample

spectrophotometry system.
● The aspirating nozzle is programmed to move in the X, Y, and Z (vertical)
directions.
● Up to 8 sets of operational parameters, including the size of racks and the
number of test tubes, may be memorized in the battery back-up protected
files.
● Up to 100 test tubes may be set together on the rack.

Note : A commercially available test tube stand, with a footprint

smaller than 220 × 220 mm, is applicable

NTT-1200P Constant-Temperature
Water Circulator
(Cat. No. 208-97242)
Circulates temperature controlled water to a constant-temperature
cell holder.
● Temperature range: Ambient +5˚C to +80˚C
● Temperature control precision: ±0.03˚C (40˚C set temperature, 20˚C
ambient)
● Max. pumping rate: 27/31 L/min, 9.5/13 m (50/60 Hz)
● External circulation nozzle: 10.5 mm OD (both outlet and return)
● Tank capacity: About 10 L (9 L during use)
● Standard accessories: Lid with handles, instruction manual
● Dimensions: 270W x 560H x 400D (mm)
● Power requirements: 100 VAC, 1250 VA, with 1.7 m power cord and
grounded plug
Note: Rubber hose (9 mm ID) (Cat. No. 016-31350-22) and hose
clamps (Cat. No. 037-61017) are not included in the
standard contents.
18
Multicell Sample Compartment
(Cat. No. 206-69160)

Holds up to six 10 mm square cells. No temperature control

capability.
● Number of cells : 6 on the sample side
1 on the reference side.

Note : Cells are not included in the standard contents.

3µL Capillary Cell Set for

Ultramicro Volume Measurement
(Cat. No. 206-69746)
● Recommended for small and precious samples, such as in biological
application. The minimum sample volume required is 3µr, when the tube
closure is used. (theoretical value)
● Solution sample is aspirated into the capillary cell and the cell is directly
subjected to measurement.
● The holder is the same size as a 10 mm square cell and can be mounted to
the standard cell holder.
● Supplied with 100 capillaries (made of quartz) and a tube closure.

Supermicro Cell Holder

(Cat. No. 206-55050-91)

Holds supermicro cells for measurement of extremely small

samples.
Samples of 25 to 200µ L can be measured, depending on the type of
black cell used.
● Applicable cells: u,u', and i in the cell list on the back cover

Specular Reflectance Measurement

Attachment (5˚ incident angle)
(Cat. No. 206-14046)
The technique of specular reflectance measurement is often utilized
for evaluation of semiconductors and optical materials relative to a
reference reflecting surface. The 5˚ incident angle minimizes the
influence of polarized light. Thus, no polarizer is required in
measurement • • • the operation is quite simple.
Optics
● Sample as large as 100W×160D×15Tmm can be readily measured, the Sample

minimum size is 7 mm in diameter.

Light beam
● Sample placement is quite easy • • • just set it on the holder with the
measuring face down.

19
 UV-1700 PharmaSpec

ACCESSORIES
GSC-3A Gel Scanner
(Cat. No. 204-51774-01)

Used to record stained bands on disk gels.

The record is given as a time-course curve, under Kinetics mode.
● Cell : Made of quartz, 6W×77.5L×11Hmm in inner dimensions. One piece is
included in the standard contents.
● Scanning range : 60 mm
● Standard contents : A main controller, a cell holder, a cell.

Constant-Temperature Cell Holder

(Cat. No. 202-30858-04)

Maintains sample and reference cells at a desired, uniform

temperature by circulating constant-temperature water.
● Temperature range : 5 to 90˚C (depends on the performance of the
constant-temperature water circulator)
● Cell holder : Accepts a pair of 10 mm square cells

Constant-Temperature Four Cell Holder

(Cat. No. 204-27206-02)

Maintains four sample cells and a reference cell at a desired, uniform

temperature by circulating constant-temperature water.
● Temperature range : 5 to 90˚C
∗ The Four-Cell Sample Compartment Unit (Cat. No. 204-00850-01) is required.

Long-Path Rectangular Cell Holder

(Cat. No. 204-23118-01)

Holds rectangular cells having an optical path of 10, 20, 30, 50, 70,
or 100mm.

Universal Rectangular Cell Holder,

Four-Cell Type
(Cat. No. 204-27208)
Permits manual change of four rectangular cells having an optical
path of 10, 20, 30, 50, 70, or 100mm.
∗ The Four-Cell Sample Compartment Unit (Cat. No. 204-00850-01) is
required.
∗ When a rectangular, long-path cell is used on the reference side, its holder
(Cat. No. 204-28720) is additionally required.

20
Cylindrical Cell Holder 10 mm Micro Flow-thru Cell with Holder
(Cat. No. 204-06216-02) (Cat. No. 204-06222)
Holds cylindrical cell having
an optical path of 10, 20, 50, or 5 mm Micro Flow-thru Cell with Holder
100 mm. (Cat. No. 204-06222-01)

Cat. No. optical path volume

204-06222-01 10mm 0.3mr
204-06222-01 5mm 0.15mr

Front Panel with holes

(Cat. No. 204-27588-03)

Allows the tubes of a flow-

thru cell, for example, to be
connected through the front
panel of the instrument.

Film Holder RS-232C Cable (Type 2)

(Cat. No. 204-58909) (Cat. No. 206-86408)
Used in transmittance This cable is required for connection of the UV-1700 to an IBM-PC
measurement of thin samples compatible personal computer.
such as films and filters. ● The cable is equipped with a 9-pin female PC connector and 9-pin male
connector for connection to the UV-1700.
● Three wires in the cable are used, 2 wires for output and 1 wire for ground.
● At the connector that connects to the PC, the pins for the control wires
allow constant input and output from the PC.

Micro Cell Holder with Mask

(Cat. No. 204-06896)

Holds a micro cell, less than 4

mm wide. (The mask width is
continuously adjustable.)

Four-Cell Sample Compartment Unit

(Cat. No. 204-00850-01)

Accommodates a four cell

holder of cylindrical,
rectangular, and other types of
cells. Incorporates a four cell
holder for 10 mm square cells.

21
 UV-1700 PharmaSpec

ACCESSORIES
8/16 Series Micro Multi-Cell
■ Cell Holder
Model Cat. No.
8/16 Series Micro Multi-cell Holder MMC-1600 206-53945-91
8/16 Series Constant Temperature
Micro Multi-cell Holder MMC-1600C 206-53900-91

This cell holder holds one micro multi-cell, either 8 or 16 cell, for
micro volume measurement.
Two types of micro multi-cell holders are available, the standard
type (MMC-1600) and the constant temperature water circulation
type (MMC-1600C).

■ Micro Multi-cells
Model Cat. No.
8 Series Micro Multi-cell; optical path length 10mm,
208-92089
cell volume 100µr
16 Series Micro Multi-cell; optical path length 10mm,
208-92088
cell volume 100µr
8 Series Micro Multi-cell; optical path length 5mm,
208-92086
cell volume 50µr
16 Series Micro Multi-cell; optical path length 5mm,
208-92085
cell volume 50µr

There are two types of micro multi-cells available in both the 8

Series and the 16 Series models, a 50µrtype and a 100µrtype. The
cell intervals of the 8 Series Micro Multi-cell are applicable for use
with 8 × 12 well microplates and 8 channel pipettes. Microplate
samples aspirated into multi channel pipettes can be injected directly
into the cells for measurement.
● Micro volume samples can be measured
(minimum sample volume: 50µror 100µr, respectively)
● Support for commercial microplates and micro pipettes.
(with 8 Series micro cells)
● Up to 16 samples can be measured at a time (with 16 Series micro cell)

Syringe Sipper
Model Cat. No. Remarks
Syringe Sipper N 206-53800-93 Normal temperature type (Flow cell available separately. Choose from the recommended flow cells listed below.)
Syringe Sipper CN 206-53800-94 Constant temperature, water circulating type (Flow cell available separately. Choose from the recommended flow cells listed below.)

Recommended Flow Cells

Cell Type Cat. No. Optical Path Length Dimensions of Aperture Standard Required Sample Volume
Square (Ultra-micro) 208-92114 10mm ø2mm 0.9mr
Square (Micro) 208-92113 10mm ø3mm 1.0mr
Square (Semi-micro) 208-92005 10mm H11×W3.5mm 5.0mr

The sipper unit employs a syringe pump system. The liquid contact
surfaces are composed of Teflon, glass, or quartz, imparting
excellent chemical resistance and ease of maintenance, and allowing
measurement of almost any sample type. Further, the extremely high
repeatability of sipping volume (repeat precision: ±0.03mr) makes it
ideal when performance validation is required.
● The flow cell can be changed independently for excellent ease of
maintenance.
● Excellent chemical resistance allows measurement of almost any sample.
● Excellent repeat sipping of fixed volumes.

22
Cells

Description Optical path (R) Type Fused silica (S) Cell Glass (G) Cell
10mm 1 200-34442 200-34565-01
20mm 6 200-34446 200-34446-01
Square cell
50mm 6 200-34944 200-34944-01
100mm 6 200-34676 200-34676-01
Square cell with stopper 10mm 2 200-34444 200-34444-01
Semi-micro cell 10mm 3 200-66501 200-66501-01
, ,
Semi-micro black cell 10mm 3 200-66551 ----
10mm 7 200-66578-11 ----
Super micro black cell , ,
5mm 7 208-92116 ----
Micro black cell 10mm 8 200-66578-12 ----
10mm 200-34448-01 (silica window) 200-34448-01 (glass window)
20mm 200-34472-01 (silica window) 200-34472-01 (glass window)
Cylindrical cell 4
50mm 200-34473-01 (silica window) 200-34473-03 (glass window)
100mm 200-34473-02 (silica window) 200-34473-04 (glass window)
1mm 200-34660-01 200-34662-01
Short path cell 2mm 5 200-34655-01 200-34662-11
5mm 200-34449-01 200-34449-01

4 4

12.5
25
45
12 12
φ
25

12
3 R 2

.5
2 2
12
.5

12.5
① ② ③ ③’ ④ ⑤ R ⑥ ⑦ ⑦’ ⑧ Unit：㎜

23
 ! WARNING
Electric Shock
Do not open the cover. To avoid
electric shock, first switch off power.

! WARNING
High Temperature
To avoid burns, do not touch or
approach too close.

SHIMADZU CORPORATION. International Marketing Division

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