Determination of Natamycin1
Determination of Natamycin1
Determination of Natamycin1
Friday 27/3/2020
Rapid Ultraviolet Spectrophotometric and Liquid Chromatographic
Methods for the Determination of Natamycin in Lactoserum Matrix
JOURNAL OF AOAC INTERNATIONAL VOL. 83, NO. 4, 2000
Definition:
Natamycin, also known as pimaricin, is a polyene antifungal drug (Chemical
Abstracts Service Registry No. 7681-93-8) with the chemical formula
C33H47NO13. The polyene compounds are so named because of the conjugated
double bonds that constitute part of their macrolide ring structure.
1- UV VIS spectrophotometer
Analysis by UV VIS:
• Blank with methanol
• Calibration curve linear withR2>0.992
10
C mg/Kg=𝐶𝑐𝑎𝑙𝑖𝑏 𝑐𝑢𝑟𝑣𝑒 ∗
2
10ml is the volume of volumetric flask so final volume of extract
2 ml is the volume of sample
Principle of UV vis spectrophotometer;
𝐴 = 𝑒𝑝𝑠𝑖𝑙𝑜𝑛 ∗ 𝐶 ∗ 𝑙
Absorbance value
Deuterium lamp for UV range
Tungsten lamp for visible range
Cmg/L working solution Absorbance
0.5 0.02
1 0.042
2 0.08
4 0.145
5 0.185 Absorbance
0.35 y = 0.0355x + 0.0054
8 0.289 R² = 0.9993
0.3
0.25
0.15
0.1
0.05
0
0 1 2 3 4 5 6 7 8 9