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    Astm E 2180 - 18

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    Fahad Khan
    This document summarizes the results of a test performed on a Mondo Sport Impact sample to determine its antimicrobial effectiveness against Methicillin-resistant Staphylococcus aureus (MRSA) according to ASTM E 2180. The test found a 4.74 log reduction and over 99.998% reduction in MRSA bacteria after 24 hours of contact with the sample. In comparison, the untreated plastic control showed no reduction. The objective of the test was to evaluate the sample's surface for antimicrobial effectiveness against MRSA as demonstrated by the ASTM test method.

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    Astm E 2180 - 18

    Uploaded by

    Fahad Khan
    125 views3 pages
    This document summarizes the results of a test performed on a Mondo Sport Impact sample to determine its antimicrobial effectiveness against Methicillin-resistant Staphylococcus aureus (MRSA) according to ASTM E 2180. The test found a 4.74 log reduction and over 99.998% reduction in MRSA bacteria after 24 hours of contact with the sample. In comparison, the untreated plastic control showed no reduction. The objective of the test was to evaluate the sample's surface for antimicrobial effectiveness against MRSA as demonstrated by the ASTM test method.

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    This document summarizes the results of a test performed on a Mondo Sport Impact sample to determine its antimicrobial effectiveness against Methicillin-resistant Staphylococcus aureus (MRSA) according to ASTM E 2180. The test found a 4.74 log reduction and over 99.998% reduction in MRSA bacteria after 24 hours of contact with the sample. In comparison, the untreated plastic control showed no reduction. The objective of the test was to evaluate the sample's surface for antimicrobial effectiveness against MRSA as demonstrated by the ASTM test method.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    125 views3 pages

    Astm E 2180 - 18

    Uploaded by

    Fahad Khan
    This document summarizes the results of a test performed on a Mondo Sport Impact sample to determine its antimicrobial effectiveness against Methicillin-resistant Staphylococcus aureus (MRSA) according to ASTM E 2180. The test found a 4.74 log reduction and over 99.998% reduction in MRSA bacteria after 24 hours of contact with the sample. In comparison, the untreated plastic control showed no reduction. The objective of the test was to evaluate the sample's surface for antimicrobial effectiveness against MRSA as demonstrated by the ASTM test method.

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    of 3

    ASTM E 2180 – 18

    Standard Test Method for Determining the Activity of Incorporated


    Antimicrobial Agent(s) in Polymeric or Hydrophobic Materials

    FINAL REPORT : R2019-303

    Prepared for:
    Mondo
    2655 Francis-Hughes
    Laval, QC H7L 3S8

    Accredited Testing Provided by:

    130 Erick Street


    Crystal Lake, IL 60014
    815.526.0954
    TESTING CERT: #2832.01

    Testing Initiated: June 26, 2019


    Testing Completed: July 1, 2019
    Report Issued: July 3, 2019

    Performed By: Marcy Aaron Approved By: Debbie Koester


    Title: Staff Scientist Title: Quality Manager
    ASTM E 2180
    MICROSTAR LAB REPORT: R2019-303
    MONDO

    Objective:
    To evaluate the surface of one sample for antimicrobial effectiveness against Methicillin-resistant
    Staphylococcus aureus (MRSA) ATCC# 43300 as demonstrated by ASTM E 2180 test method.

    Test Sample Identification:


    1. Mondo Sport Impact

    Test Procedure Summary:


    The test organism was adjusted and diluted to obtain the starting inoculum concentration in an
    agar slurry. The control was tested in triplicate at Time = 0 and Time = 24 hours. The test samples
    were tested in triplicate at Time = 24 hours. Each replicate was placed in a sterile Petri dish,
    inoculated and then incubated. At the appropriate time, the replicate was placed in sterile container
    with neutralizing broth and shaken to facilitate the release of the agar slurry to the neutralizing
    broth. Serial dilutions of the neutralizing broth containing the inoculum were plated. All plates
    were incubated. After incubation, bacterial colonies were counted and recorded. The results are
    found in the Test Results section. The results pertain only to the samples tested.
    Test Variables
    Methicillin-resistant Staphylococcus aureus
    Test Organism:
    (MRSA) ATCC# 43300

    Sample Size: 3cm x 3cm

    Pre-Cleaning: None
    Control: Untreated plastic control supplied by MicroStar
    Neutralizing Broth Used: 10 mL D/E Neutralizing Broth
    Starting Inoculum
    S. aureus (MRSA) ATCC# 43300: 4.3 x 106; Log value 6.63
    Concentration:
    Amount of Inoculum: 1.0 mL

    Contact Time: 24 hours


    Deviations from
    None, testing performed per ASTM E2180 without deviation.
    Standard Test Method:

    MSL-R-107-20DMK This accredited testing report shall not be reproduced except in full Page 2 of 3
    without written approval of The MicroStar Lab.
    ASTM E 2180
    MICROSTAR LAB REPORT: R2019-303
    MONDO

    Test Results:
    Log reduction and percent reduction is determined by comparing the treated sample after the
    contract time to the untreated plastic control after the contact time using the geometric mean
    (average of log values of each replicate) and antilog as indicated by the standard test method. The
    average number of recovered bacteria and log reduction are reported as Log10 values.

    Results against S. aureus (MRSA) ATCC# 43300 after 24-hour Contact Time
    Geometric Mean of Log Percent
    Sample Recovered Bacteria Reduction Reduction
    Untreated Plastic Control 6.44

    Mondo Sport Impact 1.70 4.74 99.998

    Percent reduction is translated into log reduction by the following:


    90% reduction = 1 log reduction; i.e. 1,000,000 (Log Value 6.00) reduced to 100,000 (Log Value 5.00)
    99% reduction = 2 log reduction; i.e. 1,000,000 (Log Value 6.00) reduced to 10,000 (Log Value 4.00)
    99.9% reduction = 3 log reduction; i.e. 1,000,000 (Log Value 6.00) reduced to 1,000 (Log Value 3.00)
    99.99% reduction = 4 log reduction; i.e. 1,000,000 (Log Value 6.00) reduced to 100 (Log Value 2.00)
    99.999% reduction = 5 log reduction; i.e. 1,000,000 (Log Value 6.00) reduced to 10 (Log Value 1.00)

    The detection limit for this test is log10 value 1.00 due to the 10x dilution that occurs with the
    addition of the neutralizing broth. Results reported as <1.00 means there were no test organisms
    found on the lowest dilution plate.

    MSL-R-107-20DMK This accredited testing report shall not be reproduced except in full Page 3 of 3
    without written approval of The MicroStar Lab.

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