Host Transmission Specifications Coulter STKS

COULTER® STKS Analyzer
COULTER® STKS Analyzer with Reticulocyte Analysis

COULTER® STKS Analyzer with CD4 and CD8 Lymphocyte Analysis
DMS 3A Host Transmission

Specifications for STKS
PN 4237290A (April 1996)
COULTER CORPORATION
Miami, Florida 33196
READ ALL PRODUCT MANUALS AND CONSULT WITH COULTER-TRAINED PERSONNEL
BEFORE ATTEMPTING TO OPERATE OR SERVICE INSTRUMENT
HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS
WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:
WARNING - Might cause injury.

CAUTION - Might cause damage to the instrument.
IMPORTANT - Might cause misleading results.
CAUTION
System integrity might be compromised and operational errors might occur:
C If you use this equipment in a manner other than specified. Operate the instrument as instructed
in the Product Manuals.
C If you introduce software that is not authorized by Coulter into your computer. Only operate your
system’s computer with software authorized by Coulter.
Coulter Corporation urges its customers to comply with all national health and safety standards, such
as the use of barrier protection. This may include, but is not limited to, protective eye wear, gloves,
and suitable laboratory attire when operating or maintaining this or any other automated laboratory
analyzer.
REVISION STATUS
Initial Issue, 04/96

Software version 3A.
This document applies to the latest software listed and higher revisions. When a subsequent software version changes information in
this document, a new issue will be released.
PN 4237290A (April 1996) iii

REVISION STATUS
iv PN 4237290A (April 1996)

CONTENT
1 INTRODUCTION, 1
1.1 DOCUMENT SCOPE AND PURPOSE, 1
1.2 INTENDED USE, 1
2 COMMUNICATION PROTOCOLS, 3
2.1 GENERAL, 3
2.2 PROTOCOLS, 3
Low Level Protocol, 3
High Level Protocol, 3
2.3 HARDWARE INTERFACE, 3

Connector Pinouts, 3
2.4 HANDSHAKE, 4
2.5 COMMUNICATION PARAMETERS, 4

Timeout, 4
Baud Rate, 5
Parity, 5
Stop Bits, 5
Handshake, 5
Block Size, 5
Graphics, 5
Compatibility, 6
Enable Spooler, 7
Replace Null by Sp, 7
PART. ASP./NO READ, 7
3 DMS TO HOST COMMUNICATIONS, 9
3.1 DATALINK, 9
Full Handshake Protocol, 9
No Handshake Protocol, 10
Data Block Structure, 10
3.2 1G1, 1G1+RETICS, AND 1G1+RETICS+SUBSETS FORMAT

PROTOCOLS, 11
Message Structure, 11
Group Definition, 12
General Information Group, 13
CBC Parameters Group, 15
DIFF Count Parameters Group, 16
DIFF Percent Parameters Group, 17
Comments Group, 18
Flag Group, 18
Demographics Group, 18
DF1 Scatterplot Group, 20
DF2 Scatterplot Group, 21
PN 4237290A (April 1996) v

CONTENT
Histogram Group, 21
RBC Histogram Group, 21
PLT Histogram Group, 22
RETICS Parameters Group, 22
DF5 LS Scatterplot Group, 24
DF6 OP Scatterplot Group, 24
Retic Histogram Group, 25
CD4 Parameters Group, 25
CD4 Scatterplot Group, 27
CD4 Histogram Group, 28
CD8 Parameters Group, 28
CD8 Scatterplot Group, 30
CD8 Histogram Group, 30
3.3 2A and 3A Format Protocols, 31

Transmission Identification, 31
Test Type Count, 31
Test Identification, 32
Group Count, 32
Group, 32
Group Number, 33
Field Count, 33
Fields, 33
Group Definition, 34
General Information Group 1, 35
CBC Parameters Group 2, 37
DIFF Count Parameters Group 3, 38
DIFF Percent Parameters Group 4, 38
Comments Group 5, 39
Definitive Flags Group 6, 39
Suspect Flags Group 7, 40
Conditional Flags Group 8, 40
Other Flags Group 9, 40
Demographics Group 10, 41
DF1 Scatterplot Group 11, 42
DF2 Scatterplot Group 12, 43
DIFF Histogram Group 13, 44
RBC Histogram Group 14, 44
PLT Histogram Group 15, 44
RETICS Parameters Group 16, 45
DF5 LS Scatterplot Group 17, 46
DF6 OP Scatterplot Group 18, 46
RETICS Histogram Group 19, 47
CD4 Parameters Group 20, 47
CD4 Scatterplot Group 21, 48
CD4 Histogram Group 22, 49
CD8 Parameters Group 23, 49
CD8 Scatterplot Group 24, 50
CD8 Histogram Group 25, 51
DIFF Latex Parameters Group 31, 52
RETIC Latex Parameters Group 32, 52
Subset Latex Parameters Group 33, 53
Control Information Group 34, 54
Control CBC Parameters Group 35, 56
Control DIFF Count Parameters Group 36, 57
Control DIFF Percent Parameters Group 37, 57
Control RETICS Parameters Group 38, 58
vi PN 4237290A (April 1996)

CONTENT
Control CD4 Parameters Group 41, 59

Control CD8 Parameters Group 42, 60
4 HOST TO DMS COMMUNICATIONS (HOST WORKLIST), 61
4.1 DATALINK, 61
Protocol, 61
Data Block Structure, 62
4.2 Presentation, 63
Message Definition, 63
APPENDIX A: ASCII TABLES, 69
A.1 Bit ASCII Codes, 69
A.2 Valid Host Communications ASCII Codes, 69
APPENDIX B: CRC, 71
B.1 CRC Algorithm, 71
B.2 CRC Example Written in ASM86, 72
B.3 CRC Example Written in C, 72
APPENDIX C: FLAGS, 73
APPENDIX D: GRAPHIC RECONSTRUCTION, 75
D.1 DF1 Valleys, 75
D.2 Diff Scatterplot, 75
D.3 Histograms, 77
APPENDIX E: RETICS GRAPHICS RECONSTRUCTION, 79
E.1 DF5, 79
E.2 DF6, 79
APPENDIX F: CD4 AND CD8 GRAPHICS RECONSTRUCTION, 81
F.1 CD4, 81
F.2 CD8, 81
PN 4237290A (April 1996) vii

CONTENT
APPENDIX G: 1G1+RETICS+SUBSETS TRANSMISSION FORMAT

EXAMPLE OF COLLATED CBC/DIFF/RETICS/CD4/CD8, 83
APPENDIX H: 3A TRANSMISSION FORMAT EXAMPLE OFCOLLATED

CBC/DIFF/RETICS/CD4/CD8, 85
APPENDIX I: 3A TRANSMISSION FORMAT EXAMPLE OF 5C CONTROL, 109
APPENDIX J: 3A TRANSMISSION FORMAT EXAMPLE OF RETIC

CONTROL, 111
APPENDIX K: 3A TRANSMISSION FORMAT EXAMPLE OF LATEX

CONTROL, 113
APPENDIX L: 3A TRANSMISSION FORMAT EXAMPLE OF CD4

CONTROL, 115
TRADEMARKS, 117
viii PN 4237290A (April 1996)

INTRODUCTION 1
1.1 DOCUMENT SCOPE AND PURPOSE

This document describes the specification for communication and data
transmission between a STKS DMS and a host computer.
1.2 INTENDED USE

This document is intended for use by software engineers needing to
either maintain or modify the operation of the STKS DMS Host
Communication.
This document will specify the DMS to host computer communication

format protocols 1G1, 1G1+Retics, 1G1+Retics+Subsets, 2A and 3A. The
specification of the host computer to DMS format protocol Host Worklist
will also be described in this document.
PN 4237290A (April 1996) 1

INTRODUCTION
2 PN 4237290A (April 1996)

COMMUNICATION PROTOCOLS 2
2.1 GENERAL
The transmission specification consists of data link (low level) and
presentation protocols (high level). Data link protocol provides the
means of transmitting data without any regard to actual information.
Presentation protocol describes the actual information and its format.
2.2 PROTOCOLS
Low Level Protocol
The STKS DMS 3A will support the Host Low Level Communication
Protocol implemented by the STKS DMS 1G1 and STKS DMS 2A.
High Level Protocol

The STKS DMS 3A supports the 1G1, 2A, 3A, MAXM (1G1+RETICS), and
1G1+Retics+Subsets Host High Level Communication Protocols. The
format of the data transmission is determined by the Compatibility
option selected on the Host Computer Definition screen. The
Compatibility option is described in more under the heading
Compatibility.
The MAXM protocol will be referred to as the 1G1+Retics protocol in
this document.
Note: To satisfy the STKS DMS 3A host requirements the receiver MUST
parse for all of the data. DO NOT ASSUME FIXED OFFSETS.
2.3 HARDWARE INTERFACE

The DMS is equipped with an auxiliary (AUX) connector on the back
side that lets the system interface with a host computer via a Standard
EIA-type 25-pin connector, and uses EIA Standard RS-232-C signals.
This is a Data Terminal Equipment (DTE) configuration.
Connector Pinouts
The DMS/Host communications uses only the following signals:
P3 Pin No. Signal Name Flow Direction

2 Transmit Data From DMS
3 Received Data To DMS
4 Ready To Send (RTS) From DMS
5 Clear To Send (CTS) To DMS
6 Data Set Ready(DSR) To DMS
7 Signal Ground Reference
8 Carrier Detect Reference
20 Data Terminal Ready (DTR) From DMS
22 Ring Indicator Reference
PN 4237290A (April 1996) 3

COMMUNICATION PROTOCOLS
The DMS is the DTE, by RS232 standards, which explains the RTS/DTR
pinouts. Whether the HOST is a DTE or DCE will determine its use of
CTS/DSR or RTS/DTR. A DTE HOST requires the use of a NULL
MODEM Cable.
Note: Pin number 5 CTS must be active for DMS to send any
transmissions. If the host computer does not support the above
hardware handshake lines it will be necessary to connect pin 4 to pin 5
and to connect pin 20 to pin 6.
2.4 HANDSHAKE
During a transmission, when in full handshake mode, host logically
raises CTS to allow the DMS to send data and logically lowers CTS to
prevent the DMS from sending data.
Note: If CTS is lowered to hold off the DMS host transmission, it must
be raised again within the DMS timeout period. If CTS is not raised
within the timeout period, the DMS transmission to the host will
timeout and abort.
DMS (sender) HOST (receiver)
<-- CTS on (send some data)

send data ----->
send data ----->
send data ----->
<-- CTS off (stop sending data)
wait
• process received data
• •
• •
• •
•
<-- CTS on (send more data)
send data ----->
send data ----->
- etc. -
2.5 COMMUNICATION PARAMETERS

The DMS allows the following communications parameters on the Host
Computer Definition screen to be configured by the user. These
parameters include communications modes, as well as parameters
enabling the transmission of graphic data.
Timeout
The time out value determines the amount of time the DMS will wait for
a response from the host before retrying to send data to the host. If the
Enable Spooler option is enabled the DMS will continue to transmit at
the time out intervals until the host successfully receives the data. If the
Enable Spooler option is not enabled the message will be aborted when
the next available message is ready to be transmitted.
The time out value can be 1 to 9 seconds. The default value is 9
seconds.
4 PN 4237290A (April 1996)

COMMUNICATION PARAMETERS
Baud Rate
The following baud rates are supported: 110, 300, 1200, 2400, 4800,
9600, 19200
Note: The slowest recommended baud rate is 2400 for non-graphic

transmissions and 9600 Baud for graphic transmissions.
Parity
Odd, Even, and None parity modes are supported. Odd parity is the
default value.
Note: The DMS/Host communications only supports the 8 bit Data Bit
mode. For Host systems that only support 7 bit data, the DMS should be
configured for No Parity and the Host should be configured for Marked
Parity for graphic transmissions.
Stop Bits
1 or 2 Stop Bits modes are supported. The default value is 2.
Handshake
If this option is set to 'Yes', the DMS looks for a handshake from the host
computer. If this option is set to 'No', there is no handshaking between
the DMS and the host computer. Refer to Handshake under heading 2.4
for more information on the handshake mode.
Block Size
The DMS/Host Communications support block sizes of 128 and 256
bytes. The default value is 256 bytes.
Graphics
If this option is set to 'Yes', the selected scatterplot(s) and histogram(s)
on the F6-Graphics window will be transmitted to the host computer. If
this option is set to 'No', scatterplot(s) and histogram(s) will not be sent
to the host computer.
Table 1 lists the F6-Graphic options that can be set to 'Yes' or 'No' to
enable or disable the graphics transmission for the corresponding test
type. Also, listed in the table are the applicable format protocols, which
allow the transmission of the corresponding graphic option. If the
graphic option is available for the specific format it will be indicated with
an 'X'.
PN 4237290A (April 1996) 5

Table 1. F6-GRAPHICS Options
Format Protocols
Test Type Graphic Options 1G1 1G1+Retics 1G1+Retics+Subsets 2A 3A
CBC RBC Histogram X X X X X
PLT Histogram X X X X X
CBC/Diff RBC Histogram X X X X X
PLT Histogram X X X X X
DF1 Scatterplot X X X X X
DF2 Scatterplot X X X X X
VCS Histograms X X X X X
Retic DF5 Scatterplot X X X X
DF6 Scatterplot X X X X
VCS Histograms X X X X
CD4 CD4 Scatterplot X X
VCS Histograms X X
CD8 CD8 Scatterplot X X
VCS Histograms X X
Compatibility
To determine which Host High Level Communication Protocol is used
for data transmission, one of the following Compatiblity options must be
selected:
1G1, 2A, 3A, MAXM (1G1 + Retics), 1G1+Retics+Subsets
Table 2 lists the compatibility options (format protocols) that are

applicable to corresponding test types. If the transmission of the test
type is available for the specific format it will be indicated with an 'X'.
Table 2. Compatibility Options
Format Protocols
Test Type 1G1 1G1+Retics 1G1+Retics+Subsets 2A 3A
CBC X X X X X
CBC/Diff X X X X X
Retics X X X X
CD4 X X
CD8 X X
6 PN 4237290A (April 1996)

COMMUNICATION PARAMETERS
Enable Spooler
If this option is set to 'Yes', the spooling function of the DMS is enabled.
When enabled, each host transmission is spooled and kept on the spool
until the Host acknowledges the transmission was successfully accepted.
Refer to the heading Full Handshake Protocol for more details on the
Enable Spooler option.
Replace Null by Sp
When this option is set to 'Yes', NULL (HEX. 0x00, DEC. 0) characters
present in the transmission buffer will be replaced by SPACES (HEX.
0x20, DEC. 32).
PART. ASP./NO READ

When this option is set to 'Yes', samples with PART. ASP. and/or
NO READ status will be transmitted to the host.
PN 4237290A (April 1996) 7

8 PN 4237290A (April 1996)

DMS TO HOST COMMUNICATIONS 3
3.1 DATALINK
All transmitted bytes are ASCII characters. All numeric values are
hexadecimal. Hence, for example, the number FF(hex) is represented by
the two ASCII bytes "FF"( 46H, 46H ). The first two bytes of the
transmission are the number of blocks to be sent, followed by
transmission of data blocks (see format below).
The final block will be padded with ASCII spaces ( 20H ) to fill a
whole block.
The DMS supports a full handshake and a no handshake protocol.
Full Handshake Protocol

The DMS sends the following control characters plus data and expects
the indicated host response:
SENDER (DMS) RECEIVER (HOST)
SYN (ready) >

< SYN (go ahead)
Block Count >

< ACK (ready to receive)
or
NAK (receiver abort)
send data blocks

>
Block
for each block < ACK (block received ok)

or
NAK (retransmit this block)
or
SYN (retransmit all blocks)

>
SYN (all done) < ACK (transmission accepted)
Up to 255 blocks of data can be sent. The actual number of blocks sent
is specified by the two byte ASCII Block Count.
If the host NAKs a data block, the block will be retransmitted. It is up to

the host to determine how many times it will retry receiving a NAKed
block before aborting.
A SYN sent by the Host at any time other than the initial "go ahead" will
force the DMS to retransmit all data starting with the first block. This
does not include the initial SYN and block count. It is up to the Host to
determine under what conditions to transmit a SYN.
PN 4237290A (April 1996) 9

DMS TO HOST COMMUNICATIONS
If the Enable Spooler option is selected, the DMS will continue to send
the same message until the Host accepts (ACKs each block) the message.
Note: To abort a transmission when the Enable Spooler option is

enabled, the host must ACK each block of the transmission and discard
it locally.
If the Enable Spooler option is not selected, the DMS will only make one
attempt at transmitting the message.
No Handshake Protocol
The No Handshake protocol ignores all hardware and software host
responses. In addition, in the No Handshake mode the DMS does not
send a SYN prior to transmitting the data.
Data Block Structure

Byte # # of bytes
1 STX 1 byte
2 BLK NBR MS CHAR 1 byte
3 BLK NBR LS CHAR 1 byte
4 DATA BYTES
•
• 256 (128) C
• byte R
259 (131) C

260 (132) CRC MSB MS CHAR 1 byte
261 (133) CRC MSB LS CHAR 1 byte
262 (134) CRC LSB MS CHAR 1 byte
263 (135) CRC LSB LS CHAR 1 byte
264 (136) ETX 1 byte
Every data block will have either 128 or 256 bytes( whichever is
chosen). If there is insufficient data to fill a block, it will be padded with
space characters.
The algorithm used to calculate the CRC for each block is a modified
CCITT CRC16. CRC is only calculated on the data bytes.
Appendix B details the algorithm and includes application notes for

implementing the algorithm in both C and assembly language.
10 PN 4237290A (April 1996)

1G1, 1G1+RETICS, AND 1G1+RETICS+SUBSETS FORMAT PROTOCOLS
3.2 1G1, 1G1+RETICS, AND 1G1+RETICS+SUBSETS FORMAT PROTOCOLS

Message Structure
The data bytes of the transmission blocks, when collected
together,exhibit the following high level format.
PREAMBLE GROUP X1 • • • • GROUP Xn POSTAMBLE
• • • •
Preamble:
C L C L C L C L C L C L - - - - - - - - - - - - - - C L
R F R F R F R F R F R F R F
The preamble marks the beginning of a message.

Group:
D FIELD C L FIELD C L FIELD C L

C COUNT R F 1 R F - - - N R F
1
The DC1 (11H) marks the beginning of a Group.

Note: If no data is present for a Group, it will be transmitted with a field
count of 00 as shown below.
If the Graphics option on the Host Computer Definition screen is set to
'No', no data including a field count of 00 will not be sent for any of the
scatterplot and histogram groups.
D 0 0 C L
C R F
1
Field Count:
FIELD COUNT defines the number of fields in a particular group. Each
Group has a Field Count which identifies the number of fields contained
within the Group. Valid field counts can be from 0 - 255. The field
count uses two characters to give the ASCII representation of the
hexadecimal value.
MS LS Two Byte ASCII representation of

CHAR CHAR Hex value between 00 and FF (0 to 255)
Fields:
FIELD defines a field within a group. Each Group is made up of variable
length fields which may or may not be padded with SP (20H), or HT
(09H) characters. A field may have a variable length TAG preceding the
data separated with one or more SP (20H), or HT (09H) characters. The
data portion of a field will only contain ASCII characters in the range of
20H to 7EH inclusive.
Fields are separated with a CR (0DH) and a LF (0AH) character.
PN 4237290A (April 1996) 11

Note: A field's position within a Group must not be used to identify it.
The CR/LF should be used to find fields and the Tag is used to
identify it.
The following notations and abbreviations are used to describe the fields:
SEP - one or more SP (20H), or HT (09H) characters.
- SP(20H)
A - Alpha characters (a..z and A..Z and space) or (61H..7AH and

41H..5AH and 20H)
N - Numeric characters (0..9, +, - and .) or (30H..39H, 2BH, 2DH and

2EH)
X - Printable characters (20H..7EH)
Postamble:
C L C L C L
R F R F - - - - - - - - - - - - - - R F
The postamble marks the end of the current message.
Group Definition
Table 3 contains the group names and the order in which the groups will
appear in the transmission message. Also, listed in the table are the applicable
test types and the format(s), which allow the transmission of the
corresponding group. If the group is available for the specific format it will be
indicated with an 'X'.
Abbreviations listed below are used to describe the corresponding test types
in the following table:
C CBC
CD CBC/DIFF
R RETICS
4 CD4
8 CD8
12 PN 4237290A (April 1996)

Table 3. 1G1, 1G1+Retics, 1G1+Retics+Subsets Group Definition and Order
Format Protocols
Group Name Group Order Test Type 1G1 1G1+Retics 1G1+Retics+Subsets

General Information 1 C,CD,R, 4,8 X X X
CBC Parameters 2 C,CD X X X
Diff Count Parameters 3 CD X X X
Diff Percent Parameters 4 CD X X X
Comments 5 C,CD,R, 4,8 X X X
Flags 6 C,CD,R, 4,8 X X X
Demographics 7 C,CD,R, 4,8 X X X
DF1 Scatterplot 8 CD X X X
DF2 Scatterplot 9 CD X X X
Diff (VCS) Histogram 10 CD X X X
RBC Histogram 11 C,CD X X X
PLT Histogram 12 C,CD X X X
Retic Parameters 13 R X X
DF5 LS Scatterplot 14 R X X
DF6 OP Scatterplot 15 R X X
Retic (VCS) Histogram 16 R X X
CD4 Parameters 17 4 X
CD4 Scatterplot 18 4 X
CD4 (VCS) Histogram 19 4 X
General Information Group
Date Field:
TAG SEP Data Format

D A T E N N / N N / N N
Data length: 9 bytes.

Data value: month/day/year.
Time Field:
TAG SEP Data Format

T I M E N N : N N : N N
Data length: 12 bytes

Data value: hours:minutes:seconds.
PN 4237290A (April 1996) 13

ID #1 Field:
TAG SEP Data Format

I D 1 X X X X X X X X X X X X X X X X
ID #2 Field:
TAG SEP Data Format

Cassette/Position Field:
TAG SEP Data Format

C A S S / P O S X X X X / X X

Data value: cassette number/cassette position.
Sequence Number Field:
TAG SEP Data Format

S E Q U E N C E X X X X X X
1E Cassette/Position Field:
TAG SEP Data Format

1 E C / P A X X X X X X

Data values for 'A':
'S' = secondary aspiration mode
' ' = primary aspiration mode
ID #1 Status Field:
TAG SEP Data Format

I D 1 S T A T U S A

'P' = positive ID
'E' = edited ID
' ' = not edited and not positive ID.
14 PN 4237290A (April 1996)

Cassette/Position Status Field:
TAG SEP Data Format

C / P S T A T U S A

'P' = positive ID
'E' = edited ID
' ' = not edited and not positive ID.
Worklist Status Field:
TAG SEP Data Format

WL S T A T U S A A A A A A A A

Data values:
'GOOD ' = OK
'PART ASP' = partial aspiration
'NO READ ' = barcode not read
'NO MATCH' = match not found in worklist
'ID MISMA' = ID mismatch
CBC Parameters Group
Each field in the CBC Parameters Group will have the following format:
TAG SEP Data Format

A A A A X X X X X X X X X
6 character results data

3 character flag data
Tags:
'WBC '
'RBC '
'HGB '
'HCT '
'MCV '
'MCH '
'MCHC '
'RDW '
'PLT '
'PCT '
'MPV '
'PDW '
Tag length: 4 bytes.
PN 4237290A (April 1996) 15

Results Data:
If the results data does not contain numeric data (e.g. xx.xx), then it contains
one of the following:
'-----' = total voteout.

'+++++' = count exceeds maximum.
'.....' = incomplete computation.
Flag Data:
The three flag data characters can contain one or a combination of the
following:
'*R' - parameter affected by other parameter

'R' - review parameter
'H' - exceeds laboratory set patient high action limit
'L' - Below laboratory set patient low action limit
'E' - edited result
' ' - no flag
DIFF Count Parameters Group
Each field in the DIFF Count Parameters Group will have the following
format:
TAG SEP Data Format


Tags:
'LY# '
'MO# '
'NE# '
'EO# '
'BA# '
Results Data:
If the results data does not contain numeric data (e.g. xx.xx), hen it contains
':::::' = flowcell clogged.

Flag Data:
following:
16 PN 4237290A (April 1996)


'L' - below laboratory set patient low action limit
'E' - edited result
' ' - no flag
DIFF Percent Parameters Group
Each field in the DIFF Percent Parameters Group will have the following
format:
TAG SEP Data Format


Tags:
'LY% '
'MO% '
'NE% '
'EO% '
'BA% '
Tag Length: 4 bytes.
Results Data:

'?????' = invalid analyzed data.
Flag Data:
following:

'E' - edited result
' ' - no flag
PN 4237290A (April 1996) 17

Comments Group
Each comment field in the Comment Group will have the following format:
Variable

X X X X X X X X C C C X X X X X X X
The Comment Group can have a variable number of fields, which consist of a
variable number of bytes. No tags are associated with a comment field. Each
comment field will consist of an ASCII string.
Flag Group
Each flag field in the Flag Group will have the following format:
Variable

X X X X X X X X C C C X X X X X X X
Data length: Variable
The Flag Group field can have a variable number of flags, which consist of a
variable number of bytes. No tags are associated with a flag. Each flag will
consist of an identifying ASCII string. All flags are listed in Appendix C Flags.
Demographics Group
Each field in the Demographics Group will have the following format:
Date of Birth Field:
TAG SEP Data Format

B I R T H N N / N N / N N N N

Data value: Birth field is month/day/year.
User Field #1:
TAG SEP Data Format

U F 1 X X X X X X X X X X X X X X X X
18 PN 4237290A (April 1996)

User Field #2:
TAG SEP Data Format

User Field #3:
TAG SEP Data Format

Sex Field:
TAG SEP Data Format

S E X A

Data value for 'A':
'F' - Female
'M' - Male
'U' - Unknown
'O' - Other
' ' - Not available
Location Field:
TAG SEP
L O C A T I O N
Data Format
X X X X X X X X X X X X X X X X
Physician Field:
TAG SEP
P H Y S I C I A N
Data Format
X X X X X X X X X X X X X X X X
User Date Field:
TAG SEP Data Format

U D A T E N N / N N / N N
PN 4237290A (April 1996) 19

User Time Field:
TAG SEP Data Format

U T I M E N N / N N / N N
DF1 Scatterplot Group
Each field in the DF1 Scatterplot Group will have the following format:
Valley 1 Field:
TAG SEP Data Format

V A L 1 N N N N N
Valley 2 Field:
TAG SEP Data Format

V A L 2 N N N N N
Valley 3 Field:
TAG SEP Data Format

V A L 3 N N N N N
Valley 4 Field:
TAG SEP Data Format

V A L 4 N N N N N
Valley 5 Field:
TAG SEP Data Format

V A L 6 N N N N N
DF1 Scatterplot Field:
TAG SEP Data Format

D I F 1 N N N C C C N N N N N N
20 PN 4237290A (April 1996)

The DF1 scatterplot data is transmitted as a 4096 byte ASCII array. See
Appendix D for details on constructing the scatterplot.
DF2 Scatterplot Group
TAG SEP Data Format

Histogram Group
Each field in the DIFF Histogram Group will have the following format:
V Histogram Field:
TAG SEP Data Format

V N N N C C C N N N N N N
C Histogram Field:
TAG SEP Data Format

C N N N C C C N N N N N N
S Histogram Field:
TAG SEP Data Format

S N N N C C C N N N N N N
RBC Histogram Group
Each field in the RBC Histogram Group will have the following format:
RBC Histogram Field:
TAG SEP Data Format

R B C H N N N C C C N N N N N N
PN 4237290A (April 1996) 21

PLT Histogram Group
Each field in the PLT Histogram Group will have the following format:
PLT Histogram Field:
TAG SEP Data Format

P L T H N N N C C C N N N N N N
PLT Fit Histogram Field:
TAG SEP Data Format

P L T F N N N C C C N N N N N N
RETICS Parameters Group
Each field in the RETICS Parameters Group will have the following format:
Retic Date Field:
TAG SEP Data Format

R E T D A T E N N / N N / N N

Retic Time Field:
TAG SEP Data Format

R E T T I M E N N : N N : N N

Retic ID Field:
TAG SEP Data Format

R I D X X X X X X X X X X X X X X X X
Note: If the MAXM (1G1+Retics) compatibility option is selected, the sample

must have a Retic ID (RID) or the following message will be displayed:
"No RETIC ID1, host transmission aborted."
22 PN 4237290A (April 1996)

Retic Cassette/Position Field:
TAG SEP Data Format

R C A S S / P O S X X X X / X X

Data value: CASS/POS output is cassette number/cassette position.
Note: This field will be transmitted only if 1G1+Retics+Subsets compatibility

option is selected.
Retic Parameter Results Field:
TAG SEP Data Format


Tags:
'RET%'
'RET#'
'MRV '
'MI '
Tag length: 4 bytes
Results Data:

'+++++' = exceeds maximum display limit.
Flag Data:
following:

'+' - exceeds parameter reportable range
'E' - edited result
'e' - calculated result from edited value
' ' - no flag
PN 4237290A (April 1996) 23

DF5 LS Scatterplot Group
Each field in the DF5 LS Scatterplot Group will have the following format:
LLS_X1 Valley:
TAG SEP Data Format

L L S 1 N N N N N
LLS_X2 Valley:
TAG SEP Data Format

L L S 2 N N N N N
DC Valley:
TAG SEP Data Format

D C N N N N N
LLS Valley:
TAG SEP Data Format

L L S 3 N N N N N
DF5 LS Scatterplot Field:
TAG SEP Data Format

Appendix E for details on constructing the scatterplot.
DF6 OP Scatterplot Group
OP3 Valley:
TAG SEP Data Format

O P 3 N N N N N
24 PN 4237290A (April 1996)

DC Valley:
TAG SEP Data Format

D C N N N N N
TAG SEP Data Format

Retic Histogram Group
Each field in the Retic Histogram Group will have the following format:
Retic V Histogram Field:
TAG SEP Data Format

R V N N N C C C N N N N N N
Retic C Histogram Field:
TAG SEP Data Format

R C N N N C C C N N N N N N
Retic S Histogram Field:
TAG SEP Data Format

R S N N N C C C N N N N N N
CD4 Parameters Group
Each field in the CD4 Parameters Group will have the following format:
CD4 Date Field:
TAG SEP Data Format

C D 4 D A T E N N / N N / N N

PN 4237290A (April 1996) 25

CD4 Time Field:
TAG SEP Data Format

C D 4 T I M E N N : N N : N N

CD4 ID Field:
TAG SEP Data Format

C D 4 I D X X X X X X X X X X X X X X X X
CD4 Cassette/Position Field:
TAG SEP Data Format

C D 4 C A S S / P O S X X X X / X X

CD4 Parameter Results Field:
TAG SEP Data Format


Tags:
'CD4%'
'CD4#'
Results Data:

Flag Data:
following:
26 PN 4237290A (April 1996)


'E' - edited result
' ' - no flag
CD4 Scatterplot Group
Each field in the CD4 Scatterplot Group will have the following format:
Valley 1:
TAG SEP Data Format

V A L 1 N N N N N
Valley 2:
TAG SEP Data Format

V A L 2 N N N N N
Valley 3:
TAG SEP Data Format

V A L 3 N N N N N
Valley 4:
TAG SEP Data Format

V A L 4 N N N N N
CD4 Scatterplot Field:
TAG SEP Data Format

C D 4 N N N C C C N N N N N N
The CD4 scatterplot data is transmitted as a 4096 byte ASCII array. See
Appendix F for details on constructing the scatterplot.
PN 4237290A (April 1996) 27

CD4 Histogram Group
Each field in the CD4 Histogram Group will have the following format:
CD4 V Histogram Field:
TAG SEP Data Format

4 V N N N C C C N N N N N N
CD4 C Histogram Field:
TAG SEP Data Format

4 C N N N C C C N N N N N N
CD4 S Histogram Field:
TAG SEP Data Format

4 S N N N C C C N N N N N N
CD8 Parameters Group
CD8 Date Field:
TAG SEP Data Format

C D 8 D A T E N N / N N / N N

CD8 Time Field:
TAG SEP Data Format

C D 8 T I M E N N : N N : N N

CD8 ID Field:
TAG SEP Data Format

C D 8 I D X X X X X X X X X X X X X X X X
28 PN 4237290A (April 1996)

CD8 Cassette/Position Field:
TAG SEP Data Format

C D 8 C A S S / P O S X X X X / X X

CD8 Parameter Results Field:
TAG SEP Data Format


Tags:
'CD8%'
'CD8#'
'4:8
Results Data:

Flag Data:
following:

'E' - edited result
' ' - no flag
PN 4237290A (April 1996) 29

CD8 Scatterplot Group
Each field in the CD8 Scatterplot Group will have the following format:
Valley 1:
TAG SEP Data Format

V A L 1 N N N N N
Valley 2:
TAG SEP Data Format

V A L 2 N N N N N
Valley 3:
TAG SEP Data Format

V A L 3 N N N N N
Valley 4:
TAG SEP Data Format

V A L 4 N N N N N
TAG SEP Data Format

The CD8 scatterplot data is transmitted as a 4096 byte ASCII array. See
Appendix F for details on constructing the scatterplot.
CD8 Histogram Group
Each field in the CD8 Histogram Group will have the following format:
CD8 V Histogram Field:
TAG SEP Data Format

8 V N N N C C C N N N N N N
30 PN 4237290A (April 1996)

2A and 3A Format Protocols
CD8 C Histogram Field:
TAG SEP Data Format

8 C N N N C C C N N N N N N
CD8 S Histogram Field:
TAG SEP Data Format

8 S N N N C C C N N N N N N
3.3 2A and 3A Format Protocols

Message Structure
The data bytes of the 2A and 3A transmission blocks, when collected
together,exhibit the following high level format.
.........................
PREAMBLE TRANSMISSION IDENTIFICATION TEST a IDENTIFICATION GROUP x 1 GROUP X n
.........................
.................. ....
TEST z IDENTIFICATION GROUP Y 1 GROUP y n POSTAMBLE
.................. ....
Preamble:
C L C L C L C L C L C L - - - - - - - - - - - - - - C L
R F R F R F R F R F R F R F
The preamble marks the beginning of a message.
Transmission Identification
S TEST C L TEST C L TEST C L

TYPE R F TYPE R F - - - TYPE R F
COUNT 1 N
The ASCII character "S" marks the beginning of the transmission.
Test Type Count
TEST TYPE COUNT defines the number of tests that will be transmitted in
this particular transmission, not all Test Types are sent in all transmissions.
Valid test type count can be from 0 - 255. The test type count uses two
characters to give the ASCII representation of the hexadecimal value.

PN 4237290A (April 1996) 31

Test Type:
Each TEST TYPE listed below is identified by the order in which it appears
after the preamble. The test types will never be transmitted out of order.
The Tests types are up to 32 characters long. Available tests/controls are as

follows:
TEST Test Type

CBC "CBC"
DIFF "DIFF"
RETICS "RETIC"
CD4 "CD4"
CD8 "CD8"
5C Control "5CC"
4C® Control "4CC"
Retic Control "RETICC"
Latex Control "LATEXC"
CD4 Control "CD4C"
CD8 Control "CD8C"
Test Identification
T TEST C L GROUP C L
TYPE R F COUNT R F
The ASCII character "T" marks the beginning of a test identification.
For a description of the TEST TYPE see above heading Test Type.
Note: When there is no data for a Group, the Group will not be sent.
Group Count
GROUP COUNT defines the number of groups of data in this particular TEST
TYPE. Valid group counts can be from 0 - 255. The group count uses two

Group
G GROUP C L FIELD C L FIELD C L

NUMBER R F COUNT R F - - - N R F
The ASCII character "G" marks the beginning of a group.
32 PN 4237290A (April 1996)

Group Number
GROUP NUMBER defines the group number contained within the group
identifier. Valid group number can be from 0 - 255. The group number uses
two characters to give the ASCII representation of the hexadecimal value.

Field Count
FIELD COUNT defines the number of fields in a particular group, see field
count. Valid field counts can be from 0 - 255. The field count uses two

Fields
FIELD defines a field within a group. A field may have a variable length TAG
preceding the data separated with one or more SP (20H), or HT (09H)
characters. The data portion of a field will only contain ASCII characters in
the range of 20H to 7EH inclusive.
Note: A field's position within a Group must not be used to identify it. The
CR/LF should be used to find fields and the Tag is used to identify it.
Appendix A tabulates the valid ASCII characters.
Fields are separated with a CR (0DH) and a LF (0AH) character.
The following notations and abbreviations are used to describe the fields:
SEP - one or more SP (20H), or HT (09H) characters.
- SP(20H)
A - Alpha characters (a..z, A..Z and space) or (61H..7AH, 41H..5AH,

and 20H)
N - Numeric characters (0..9, + -, and .) or (30H..39H, 2BH, 2DH,

and 2EH)
X - Printable characters (20H..7EH)
Postamble:
C L C L C L
R F R F - - - - - - - - - - - - - - R F
The postamble marks the end of the current message.
PN 4237290A (April 1996) 33

Group Definition
Table 4 contains the group names and their corresponding group numbers.
Also, listed in the table are the applicable test types and the format(s), which
allow the transmission of the corresponding group. If the group is available
for the specific format it will be indicated with an 'X'.
Abbreviations listed below are used to describe the corresponding test types
in the following table:
C CBC
C DCBC/DIFF
R RETICS
4 CD4
8 CD8
Cx 4C CONTROL
Dx 5C CONTROL
Rx RETIC CONTROL
L LATEX CONTROL
4x CD4 CONTROL
8x CD8 CONTROL
Note: '+' indicates that the group information will not be repeated for each
test type.
Table 4. 2A and 3A Group Definition
Group Format Protocol

Group Name Number Test Type 2A 3A
General Information 1 C,CD,R, 4,8 X X
CBC Parameters 2 C,CD X X
Diff Count Parameters 3 CD X X
Diff Percent Parameters 4 CD X X
Comments+ 5 C,CD,R, 4,8 X X
Definitive Flags+ 6 C,CD,R, 4,8 X X
Suspect Flags+ 7 C,CD,R, 4,8 X X
Conditional Flags+ 8 C,CD,R, 4,8 X X
Other Flags+ 9 C,CD,R, 4,8 X X
Demographics+ 10 C,CD,R, 4,8 X X
DF1 Scatterplot 11 CD X X
DF2 Scatterplot 12 CD X X
Diff (VCS) Histogram 13 CD X X
RBC Histogram 14 C,CD X X
PLT Histogram 15 C,CD X X
Retic Parameters 16 R X X
DF5 LS Scatterplot 17 R X X
DF6 OP Scatterplot 18 R X X
Retic (VCS) Histogram 19 R X X
34 PN 4237290A (April 1996)

Table 4. 2A and 3A Group Definition
Format Protocols
Group
Group Name Number Test Type 2A 3A
DIFF Latex Parameters 31 L X X
Retic Latex Parameters 32 L X X
Subset Latex Parameters 33 L X X
Control Information 34 Cx,CDx,Rx, X X
L,4x,8x
Control CBC Parameters 35 Cx,CDx X X
Control DIFF Count 36 CDx X X
Parameters
Control DIFF Percent 37 CDx X X
Parameters
Control RETICS Parameters 38 Rx X X
Control CD4 Parameters 41 4x X
Control CD8 Parameters 42 8x X
General Information Group 1
Date Field:
TAG SEP Data Format


Time Field:
TAG SEP Data Format


ID #1 Field:
TAG SEP Data Format

PN 4237290A (April 1996) 35

Pre Assigned ID #1 Field:
TAG SEP Data Format

P I D 1 X X X X X X X X X X X X X X X X
TAG SEP Data Format

C A S S P O S A X X X X X X

'S'-secondary aspiration mode
'P'-Primary aspiration mode
Pre-Assigned Cassette/Position Field:
TAG SEP Data Format

P C A S S P O S X X X X X X

ID #1 Status Field:
TAG SEP Data Format

I D 1 S T A T U S A
Data length: 1 byte

'P' - positive id
'E' - edited id
Cassette/Position Status Field:
TAG SEP Data Format

C / P S T A T U S A
Data length: 1 byte

Data values:
'P' - positive id
'E' - edited id
Sample Status:
TAG SEP Data Format

S A S T A T U S A A A A A A A A

Data values:
'PART ASP' - partial aspiration
'NO READ ' - barcode not read
'NO MATCH' - match not found in worklist
'ID MISMATCH' - positive id did not match.
36 PN 4237290A (April 1996)

CBC Parameters Group 2
Each field in the CBC Parameters Group will have the following format:
TAG SEP Data Format
A A A •• A X X X X X X X X

1 character (space) separator
3 character flag data.
Tags:
'WBC'
'RBC'
'HGB'
'HCT'
'MCV'
'MCH'
'MCHC'
'RDW'
'PLT'
'PCT'
'MPV'
'PDW'
Tag length: variable
Results Data:

Flag Data:
following:
'*R' - parameter affected by other parameter

'E' - edited result
' ' - no flag
PN 4237290A (April 1996) 37

DIFF Count Parameters Group 3
Each field in the DIFF Count Parameters Group will have the following
format:
TAG SEP Data Format


Tags:
'LY#'
'MO#'
'NE#'
'EO#'
'BA#'
Tag length: variable.
Results Data:

Flag Data:
following:

'E' - edited result
' ' - no flag
DIFF Percent Parameters Group 4
Each field in the DIFF Percent Parameters Group will have the following
format:
TAG SEP Data Format


5 character results data max.
1 character (space) separator min.
3 character flag data max.
38 PN 4237290A (April 1996)

Tags:
'LY%'
'MO%'
'NE%'
'EO%'
'BA%'
Results Data:

Flag Data:
following:

'E' - edited result
' ' - no flag
Comments Group 5
Note: This group will be transmitted with the first test only.
Comment Field:
TAG SEP Data Format

C O M M E N T X X X ••••• X X X
The Comment Group can have a variable number of fields, which consist of a
variable number of bytes. Each comment field will consist of an ASCII string.
Definitive Flags Group 6
Definitive Flag Field:
TAG SEP Variable

D E F I N I T X X X X X X X X C C C X X X X X X X
PN 4237290A (April 1996) 39

The Definitive Flag Group field can have a variable number of flags, which
consist of a variable number of bytes. Refer to Appendix C Flags for possible
Definitive flag values.
Suspect Flags Group 7
Suspect Flag Field:
TAG SEP Variable

S U S P E C T X X X X X X X X C C C X X X X X X X
The Suspect Flag Group field can have a variable number of flags, which
Suspect flag values.
Conditional Flags Group 8
Conditional Flag Field:
TAG SEP Variable

C O N D I T I O N X X X X X X X X C C C X X X X X X X
The Conditional Flag Group field can have a variable number of flags, which
Conditional flag values.
Other Flags Group 9
Other Flag Field:
TAG SEP Variable

O T H E R X X X X X X X X C C C X X X X X X X
The Other Flag Group field can have a variable number of flags, which consist
of a variable number of bytes. Refer to Appendix C Flags for possible Other
flag values.
40 PN 4237290A (April 1996)

Demographics Group 10
Date of Birth Field:
TAG SEP Data Format

B I R T H N N / N N / N N N N

User Field #1:
TAG SEP Data Format

User Field #2:
TAG SEP Data Format

User Field #3:
TAG SEP Data Format

Sex Field:
TAG SEP Data Format

S E X A
Data length: 1 byte
Data value:
'M' - Male
'F' - Female
'U' - Unknown
'O' - Other
Location Field:
TAG SEP Data Format

L O C A T I O N X X X X X X X X X X X X X X X X
PN 4237290A (April 1996) 41

Physician Field:
TAG SEP
P H Y S I C I A N
Data Format
X X X X X X X X X X X X X X X X X X X X X X
User Entry Date Field:
TAG SEP Data Format

E D N N / N N / N N
User Entry Time Field:
TAG SEP Data Format

E T N N : N N
ID #2 Field:
TAG SEP Data Format

Sequence Number:
TAG SEP Data Format

S E Q U E N C E X X X X X X
Profile Field:
TAG SEP Data Format

P F N
Data length: 1 byte

Data value: '1' through '9'. Note: If '0' this field will not be transmitted.
DF1 Scatterplot Group 11
Valley 1 Field:
TAG SEP Data Format

V A L 1 N N N
42 PN 4237290A (April 1996)

Valley 2 Field:
TAG SEP Data Format

V A L 2 N N N
Valley 3 Field:
TAG SEP Data Format

V A L 3 N N N
Valley 4 Field:
TAG SEP Data Format

V A L 4 N N N
Valley 5 Field:
TAG SEP Data Format

V A L 5 N N N
TAG SEP Data Format

D F 1 N N N C C C N N N N N N
DF2 Scatterplot Group 12
TAG SEP Data Format

PN 4237290A (April 1996) 43

DIFF Histogram Group 13
V Histogram Field:
TAG SEP Data Format

C Histogram Field:
TAG SEP Data Format

S Histogram Field:
TAG SEP Data Format

N N N C C C N N N N N N
RBC Histogram Group 14
RBC Histogram Field:
TAG SEP Data Format

R B C H N N N C C C N N N N N N
PLT Histogram Group 15
PLT Histogram Field:
TAG SEP Data Format

P L T H N N N C C C N N N N N N
PLT Fit Histogram Field:
TAG SEP Data Format

P L T F N N N C C C N N N N N N
44 PN 4237290A (April 1996)

RETICS Parameters Group 16
Each field in the RETICS Parameters Group will have the following format:
TAG SEP Data Format

Tags:
'RET%'
'RET#'
'MRV'
'MI'
'OTHER'
Results Data:

'MRV and MI parameters are NOT for Diagnostic Use.' = data value for
OTHER tag only.
Flag Data:
following:

'E' - edited result
' ' - no flag
Diagnostics Parameters:
MRV, MI and OTHER tags are only sent when a Retics II or Retics III options
are installed.
PN 4237290A (April 1996) 45

DF5 LS Scatterplot Group 17

LLS_X1 Valley:
TAG SEP Data Format
L L S 1 N N N

LLS_X2 Valley:
TAG SEP Data Format
L L S 2 N N N
DC Valley:
TAG SEP Data Format
D C N N N
LLS Valley:
TAG SEP Data Format
L L S 3 N N N
DF5 LS Scatterplot Field:

TAG SEP Data Format

The LS Scatterplot Data is transmitted as a 4096 byte ASCII array. See
DF6 OP Scatterplot Group 18

OP 3 Valley:
TAG SEP Data Format
O P 3 N N N
DC Valley:
TAG SEP Data Format
D C N N N
46 PN 4237290A (April 1996)

DF6 OP Scatterplot Field:
TAG SEP Data Format

D F 6 H H H C C C H H H H H H
The OP Scatterplot Data is transmitted as a 4096 byte ASCII array. See

RETICS Histogram Group 19
V Histogram Field:
TAG SEP Data Format

C Histogram Field:
TAG SEP Data Format

S Histogram Field:
TAG SEP Data Format

CD4 Parameters Group 20
TAG SEP Data Format

Tags:
'CD4%'
'CD4#'
'CD4:CD8'
PN 4237290A (April 1996) 47

Tag Table length: variable
Results Data:
'+++++' = result exceeds maximum display limit.

'?????' = invalid percent result.
Flag Data:
following:

'E' - edited result
' ' - no flag
CD4 Scatterplot Group 21
Valley 1 Field:
TAG SEP Data Format

V A L 1 N N N
Valley 2 Field:
TAG SEP Data Format

V A L 2 N N N
Valley 3 Field:
TAG SEP Data Format

V A L 3 N N N
Valley 4 Field:
TAG SEP Data Format

V A L 4 N N N
48 PN 4237290A (April 1996)

Valley 5 Field:
TAG SEP Data Format

V A L 5 N N N
TAG SEP Data Format

The CD4 Scatterplot Data is transmitted as a 4096 byte ASCII array.

See Appendix F for details on constructing the scatterplot.
CD4 Histogram Group 22
V Histogram Field:
TAG SEP Data Format

C Histogram Field:
TAG SEP Data Format

S Histogram Field:
TAG SEP Data Format

CD8 Parameters Group 23
TAG SEP Data Format

PN 4237290A (April 1996) 49

Tags:
'CD8%'
'CD8#'
Results Data:
'+++++' = result exceeds maximum display limit.

'?????' = invalid percent result.
Flag Data:
following:

'E' - edited result
' ' - no flag
CD8 Scatterplot Group 24
Valley 1 Field:
TAG SEP Data Format

V A L 1 N N N
Valley 2 Field:
TAG SEP Data Format

V A L 2 N N N
Valley 3 Field:
TAG SEP Data Format

V A L 3 N N N
50 PN 4237290A (April 1996)

Valley 4 Field:
TAG SEP Data Format

V A L 4 N N N
Valley 5 Field:
TAG SEP Data Format

V A L 5 N N N
TAG SEP Data Format

The CD8 Scatterplot Data is transmitted as a 4096 byte ASCII array.

See Appendix F for details on constructing the scatterplot.
CD8 Histogram Group 25
V Histogram Field:
TAG SEP Data Format

C Histogram Field:
TAG SEP Data Format

S Histogram Field:
TAG SEP Data Format

PN 4237290A (April 1996) 51

DIFF Latex Parameters Group 31

Each field in the DIFF Latex Parameters Group will have the following
format:
TAG SEP Data Format
A A A •• A X X X X X X


Tags:
'PRIMER'
'V_MN'
'C_MN'
'S_MN'
'V_CV'
'C_CV'
'S_CV'
Results Data:
Flag Data:
'H' = Greater than Expected Value Range.
'L' = Less than Expected Value Range.
RETIC Latex Parameters Group 32

Each field in the RETIC Latex Parameters Group will have the following
format:
TAG SEP Data Format

52 PN 4237290A (April 1996)

Tags:
'PRIMER'
'V_MN'
'C_MN'
'S_MN'
'V_CV'
'C_CV'
'S_CV'
Results Data:

Flag Data:

Subset Latex Parameters Group 33
Each field in the Subset Latex Parameters Group will have the following
format:
TAG SEP Data Format

Tags:
'PRIMER'
'V_MN'
'C_MN'
'S_MN'
'V_CV'
'C_CV'
'S_CV'
Results Data:
PN 4237290A (April 1996) 53


Flag Data:

Control Information Group 34
Run# Field:
TAG SEP Data Format

R U N # X X X
Lot# Field:
TAG SEP Data Format

L O T # N N N N N N
User Control Name Field:
TAG SEP Data Format

U C N A M E X X X X X X X X X X X X
Expiration Date Field:
TAG SEP Data Format

E X P D A T E N N / N N / N N

Date Field:
TAG SEP Data Format


Time Field:
TAG SEP Data Format


54 PN 4237290A (April 1996)

Operator Field:
TAG SEP Data Format

O P E R A T O R X X X
IQAP ID Field:
TAG SEP Data Format

I Q A P X X X X X X X X X X X X
Delete Flag Field:
TAG SEP Data Format

D F L A G A
Data length: 1 byte

Data Value:
'Y' - Run was deleted from Control Review or Report Screen.
Shift Field:
TAG SEP Data Format

S H I F T N
Data length: 1 byte

Data values:
'0', '1', '2' or '3'.
TAG SEP Data Format

C A S S P O S A X X X X X X

Data value: cassette number/cassette position
Data value for A:

S - secondary aspiration mode
- primary aspiration mode
Reference RBC Count Field:
TAG SEP Data Format

R R B C X X X X X

Note: RRBC field is applicable only to Retic controls.
PN 4237290A (April 1996) 55

Reference WBC Count Field:
TAG SEP Data Format

R W B C X X X X X

Note: RWBC field is applicable only to CD4 and/or CD8 controls.
Control CBC Parameters Group 35
Each field in the Control CBC Parameters Group will have the following
format:
TAG SEP Data Format

Tags:
'WBC'
'RBC'
'HGB'
'HCT'
'MCV'
'MCH'
'MCHC'
'RDW'
'PLT'
'PCT'
'MPV'
'PDW'
Results Data:

56 PN 4237290A (April 1996)

Flag Data:

Control DIFF Count Parameters Group 36
Each field in the Control DIFF Count Parameters Group will have the
following format:
TAG SEP Data Format

Tags:
'LY#'
'MO#'
'NE#'
'EO#'
'BA#'
Results Data:

Flag Data:

Control DIFF Percent Parameters Group 37
Each field in the Control DIFF Count Parameters Group will have the
following format:
PN 4237290A (April 1996) 57

TAG SEP Data Format


Tags:
'LY%'
'MO%'
'NE%'
'EO%'
'BA%'
Results Data:

Flag Data:

Control RETICS Parameters Group 38
Each field in the Control RETICS Parameters Group will have the following
format:
TAG SEP Data Format

58 PN 4237290A (April 1996)

Tags:
'RET%'
'RET#'
'MRV'
'MI'
'OTHER'
Results Data:

'MRV and MI parameters are Not for Diagnostic Use.' = Data value for
OTHER tag only.
Flag Data:

Diagnostics Parameters:
MRV, MI, and OTHER tags are only sent when a Retics II or Retics III
options are installed.
Control CD4 Parameters Group 41
Each field in the Control CD4 Parameters Group will have the following
format:
TAG SEP Data Format

Tags:
'CD4%'
'CD4#'
PN 4237290A (April 1996) 59

Results Data:

Flag Data:

Control CD8 Parameters Group 42
Each field in the Control CD8 Parameters Group will have the following
format:
TAG SEP Data Format

Tags:
'CD8%'
'CD8#'
Results Data:

Flag Data:

60 PN 4237290A (April 1996)

HOST TO DMS COMMUNICATIONS (HOST WORKLIST) 4
4.1 DATALINK
Protocol
The DMS requires full handshaking to receive data. The protocol is similar to
that used for DMS to Host transmissions with the SYN character replaced
with the ENQ character.
The data is transmitted as a sequence of up to 255 blocks of data of 256 [or

128] bytes each. Generally, these blocks contain 256 data bytes each, but due
to the unique needs of differing hosts, the system is configurable to allow
shorter blocks with 128 data bytes.
Sender (host computer) Receiver

(DMS/Digiboard)
(SYN indicates receiver
busy. Prepare to receive
sample(s) record.)
ENQ > < ENQ/SYN
X X >
2 byte data block
< ACK/NAK
(NAK indicates Receiver Abort)
2-byte Exactly 256 or 128 4-byte

STX ETX
blk number data bytes CRC
Send block and await response. Repeat data block and response until done.
< ACK/NAK/ENQ
After each block

ENQ >
"All Done"
< DLE Accept /

ACK/NAK/ENQ Reject
The last ACK/NAK sent by DMS/Digiboard indicates to the Host whether

DMS/Digiboard accepts or rejects the entire transmission at the Data Link
level. DMS/Digiboard than transmits a DLE character followed by a single
ASCII character indicating whether the transmission is accepted or rejected at
PN 4237290A (April 1996) 61

HOST TO DMS COMMUNICATIONS (HOST WORKLIST)
the presentation level. The ASCII character following the DLE may be one of
the following:
Transmission Accepted, Ready for next: 'A'
Transmission Accepted, DO NOT send more: 'B'
Transmission Rejected, Please retry: 'C'
Transmission Rejected, Please abandon: 'D'
The following demonstrates the above protocol:

SENDER (DMS) RECEIVER (HOST)
ENQ (ready) >
< ENQ (go ahead)
or
SYN (busy)
# of data blocks (2 bytes) >
< ACK (ready to receive)
or
NAK (receiver abort)
send data blocks
Block
>
for each block
< ACK (block received ok)
or
NAK (retransmit this block)

or
ENQ (all done) ENQ (retransmit all blocks)
>
< ACK (transmission accepted)
or
NAK (transmission rejected)
(Presentation Level Response)
< DLE
< A (transmission accepted, ready for next)
or
B (transmission accepted, do Not send more)
or
C (transmission rejected, retry)
or
D (transmission rejected, abandon)
Data Block Structure

Byte # # of bytes
1 STX 1 byte
2 BLK NBR MS CHAR 1 byte
3 BLK NBR LS CHAR 1 byte
4 DATA BYTES
• 256 (128) C
• byte R
• C

260 (132) CRC MSB MS CHAR 1 byte
261 (133) CRC MSB LS CHAR 1 byte
262 (134) CRC LSB MS CHAR 1 byte
263 (135) CRC LSB LS CHAR 1 byte
264 (136) ETX 1 byte
62 PN 4237290A (April 1996)

Presentation
4.2 Presentation
Message Structure
PREAMBLE INTRO FIELD 1 • • • FIELD N
The data which is blocked for transmission will consist of a sequence of ASCII
lines each terminated by a CR/LF pair. The block boundaries for the 256 [or
128] byte blocks would have no special significance with respect to this data.
The data may be thought of as a large ASCII buffer which is being sent 256
[or 128] bytes at a time.
Preamble
Field SH 2-byte CR LF
num fileds
"00" to "FF"
Intro
Field 2 byte 2-byte CR LF
record type operation
Each
Field ASCII ASCII CR LF Repeat until all
Tag Data fields given
Possible Record Type Value: WL - Worklist
Possible Operation Type Value: AD - Add this record
The 2-byte num fields indicate the number of fields that follow the preamble
field, i.e, the intro field plus all data fields.
The record type indicates the target data set, e.g, "WL" for Worklist.
The operation code indicates the action to be performed, e.g, "AD" for add
this record.
The ASCII Tag is unique to each field within each record type and is always 2
bytes. The ASCII tag TS can repeat itself for as many tests as may be required.
Message Definition
The Host Work List consists of the information about a number of blood
samples, each of which has a number of fields. Internally the structure is the
same as that used for the Active WorkList. Most of these fields may be
transmitted from the Host to the DMS. Below is the list of possible fields,
their field width and the appropriate ASCII Tag to be used for transmission.
IMPORTANT: FIELDS CANNOT HAVE LEADING OR TRAILING SPACES

(MESSAGE WILL BE REJECTED).
PN 4237290A (April 1996) 63

Cassette Number & Position Field:
Tag Data Format

C P N N N N N N
Data length : 6 bytes maximum
Note: 1G1, MAXM (1G1+Retics), and 1G1+Retics+Subsets PROTOCOL

SPECIFIC. THE 1G1, MAXM (1G1+Retics), or 1G1+Retics+Subsets
COMPATIBILITY OPTION MUST BE SELECTED, OTHERWISE THIS
FIELD WILL BE IGNORED BY THE HOST WORKLIST.
Identifier 1 Field:
Tag Data Format

I 1 X X X X X X X X X X X X X X X X
Data length: 16 bytes maximum

Identifier 2 Field:
Tag Data Format

I 2 X X X X X X X X X X X X X X X X
Sequence Number Field:
Tag Data Format

S N N N N N N N
Birth Date Field:
Tag Data Format

B D N N / N N / N N N N

Data value: month/day/year
Example: 12/31/1999
Sex Field:
Tag Data Format

S X A
64 PN 4237290A (April 1996)

Presentation
Data length: 1 byte maximum

Data value:
'M' - Male
'F' - Female
'U' - Unknown
'O' - Other
Location Field:
Tag Data Format

L N X X X X X X X X X X X X X X X X
Physician Field:
Tag Data Format

P H X X X X X X X X X X X X X X X X X X X X X X
User Field 1 Field:
Tag Data Format

U 1 X X X X X X X X X X X X X X X X
User Field 2 Field:
Tag Data Format

User Field 3 Field:
Tag Data Format

Comments 1 Field:
Tag Data Format

C 1 X X X X X C C C X X X X X

PN 4237290A (April 1996) 65

Comments 2 Field:
Tag Data Format

C 2 X X X X X C C C X X X X X

Comment Field:
Tag Data Format

C M X X X X X C C C X X X X X
Note: COMMENT 1, COMMENT 2 - For 1G1, MAXM (1G1+Retics), or

1G1+Retics+Subsets protcols, these two items (C1, C2) are concatenated and
called comment (CM) ONLY IF the 1G1 or MAXM (1G1+Retics), or
1G1+Retics+Subsets Compatibility option is selected, otherwise C1 and/or C2
are ignored.
Profile Field:
Tag Data Format

P F N
Data values: '1' through '9'. Note: If 1G1, MAXM (1G1+Retics) or

1G1+Retics+Subsets Compatibility option is selected, this value will contain '1'.
Entry Date Field:
Tag Data Format

E T N N / N N / N N

Data value: month/day/year
Example: 12/31/99
Entry Time Field:
Tag Data Format

E T N N : N N

Data value: hour:minute
Example: 20:59
66 PN 4237290A (April 1996)

Presentation
Test Field:
Tag Data Format

R E X X X X X , X X X X X X X X X X X X X X X X , X X X X X X

5 character Test max.

1 character (comma) separator
16 character ID1 max.
1 character (comma) separator
6 character numeric Cassette/Position
Data values: TEST,ID1,CASSETTE/POSITION
Test values:
'CBC' (CBC test only)
'DIFF' (CBC/Diff test)
'RETIC'
'CD4'
'CD8'
Examples:
TSRETIC,, Invalid, ID1 or Cassette/Position not given.
TSRETIC,,000204 OK, ID1 not given.
TSRETIC,1234567, OK, Cassette/Position not given.
TSRETIC,1234567,000204 OK, Both are specified.
TSCD8,22338388,000105 OK, Both are specified.
Note: 2A AND 3A PROTOCOL SPECIFIC. THE 2A OR 3A COMPATIBILITY

OPTION MUST BE SELECTED, OTHERWISE THIS FIELD WILL BE
IGNORED BY THE HOST WORKLIST.
This field includes the test type and its corresponding ID1 and/or
Cassette/Position. The host may transmit up to five test fields for CBC, DIFF,
RETIC, CD4, and CD8 in any order.
IMPORTANT: Commas are required as separators. The sample will be

rejected if any of the commas, test type, or a positive identifier is missing.
Sample Mode Field:
Tag Data Format

S M A
Data length: 1 byte maximum

Data values:
'P' - primary aspiration mode
'S' - secondary aspiration mode

PN 4237290A (April 1996) 67

68 PN 4237290A (April 1996)

APPENDIX A
ASCII TABLES
A.1 Bit ASCII Codes

0 0 0 0 1 1 1 1
0 0 1 1 0 0 1 1
0 1 0 1 0 1 0 1
? ? ?
7 6 5 4 3 2 1

X X X 0 0 0 0 NUL DLE SP 0 @ P ` p
X X X 0 0 0 1 SOH DC1 ! 1 A Q a q
X X X 0 0 1 0 STX DC2 " 2 B R b r
X X X 0 0 1 1 ETX DC3 # 3 C S c s
X X X 0 1 0 0 EOT DC4 $ 4 D T d t
X X X 0 1 0 1 ENQ NAK % 5 E U e u
X X X 0 1 1 0 ACK SYN & 6 F V f v
X X X 0 1 1 1 BEL ETB ' 7 G W g w
X X X 1 0 0 0 BS CAN ( 8 H X h x
X X X 1 0 0 1 HT EM ) 9 I Y i y
X X X 1 0 1 0 LF SUB * : J Z j z
X X X 1 0 1 1 VT ESC + ; K [ k {
X X X 1 1 0 0 FF FS , < L \ l |
X X X 1 1 0 1 CR GS - = M ] m }
X X X 1 1 1 0 SO RS . > N ^ n ~
X X X 1 1 1 1 SI US / ? O _ o DEL

A.2 Valid Host Communications ASCII Codes

0 0 0 0 1 1 1 1
0 0 1 1 0 0 1 1
0 1 0 1 0 1 0 1
? ? ?
7 6 5 4 3 2 1

X X X 0 0 0 0 DLE SP 0 @ P ` p
X X X 0 0 0 1 ! 1 A Q a q
X X X 0 0 1 0 STX " 2 B R b r
X X X 0 0 1 1 ETX # 3 C S c s
X X X 0 1 0 0 $ 4 D T d t
X X X 0 1 0 1 ENQ NAK % 5 E U e u
X X X 0 1 1 0 ACK SYN & 6 F V f v
X X X 0 1 1 1 ' 7 G W g w
X X X 1 0 0 0 ( 8 H X h x
X X X 1 0 0 1 HT ) 9 I Y i y
X X X 1 0 1 0 LF * : J Z j z
X X X 1 0 1 1 + ; K [ k {
X X X 1 1 0 0 , < L \ l |
X X X 1 1 0 1 CR - = M ] m }
X X X 1 1 1 0 . > N ^ n ~
X X X 1 1 1 1 / ? O _ o

Highlighted only used for Datalink control.
PN 4237290A (April 1996) 69

ASCII TABLES
70 PN 4237290A (April 1996)

APPENDIX B
CRC
B.1 CRC Algorithm

The CRC algorithm used is a modified CCITT CRC16 algorithm. The
polynomial for this algorithm is:
X**16 + X**12 + X**5 + 1.
Note: D = current data byte that is input to the algorithm.

CRCLSB,CRCMSB = data byte. Least significant and most significant
CRC accumulator bytes.
x>>n means x is shifted n bits to right or is the same as x divided by 2n .
x<<n means x is shifted n bits to left or is the same as x multiplied by 2n .
at beginning,
CRCLSB = 0FFH (octal 377) (decimal 255)

CRCMSB = 0FFH (octal 377) (decimal 255)
then for each data byte in a block,
X = D XOR CRCMSB
X = X XOR ( X >> 4 )
CRCMSB = CRCLSB XOR ( X >> 3 ) XOR ( X << 4 )
CRCLSB = X XOR ( X << 5 )
and at end,
CRCLSB = CRCLSB XOR 0FFH

CRCMSB = CRCMSB XOR 0FFH.
PN 4237290A (April 1996) 71

CRC
B.2 CRC Example Written in ASM86

The following is an example of CRC16 code written in assembler for 8086:
;----------------------------------------------------------------
-------------GET_CRC_BYTES PROC
PUSH CX
;This is the Algorithm used (CCITT CRC16):
;
; X = D XOR CRCMSB
; X = X XOR ( X >> 4 )
; CRCMSB = CRCLSB XOR ( X >> 3 ) XOR ( X << 4 )
; CRCLSB = X XOR ( X << 5 )
;
;Data arrives in AL.
;Finished CRC is in BX ( BH = MSB, BL = LSB )
;To use this algorithm:
; 1. Initialize BX to 0FFFFH.
; 2. At end:
; CRCLSB = CRCLSB XOR 0FFH.
; CRCMSB = CRCMSB XOR OFFH.
XOR AL,BH ;X=D XOR CRCMSB (AL=X)
<--- 1
MOV AH,AL ;Save X for later in AH
SHR AL,4 ;Then AL=X >> 4
XOR AL,AH ;X=X XOR ( X >> 4 )
<--- 2
MOV AH,AL ;Save X
MOV CH,AL ;Save X
SHR AL,3 ;Then AL= ( X >> 3 )
XOR AL,BL ;AL=CRCLSB XOR ( X >> 3 )
SHL AH,4 ;AH=( X << 4 )
XOR AL,AH ;CRCMSB=CRCLSB XOR
MOV BH,AL ; ( X >> 3 ) XOR ( X << 4 )
<--- 3
MOV AL,CH ;Recover X in AL
SHL AL,5 ;AL=( X << 5 )
XOR AL,CH ;AL=X XOR ( X << 5 )
MOV BL,AL ;CRCLSB=X XOR ( X << 5 )
<--- 4
POP CX
RET
GET_CRC_BYTES ENDP
;--------------------------------------------------------------
B.3 CRC Example Written in C

The following is an example of CRC16 code written in C.
void calc_crc ( unsigned character data_byte )

{
data_byte ^= crcmsb;
data_byte ^= ( data_byte / 16 );
crcmsb = crclsb ^ ( data_byte / 8 ) ^ (
data_byte * 16 );
crclsb = data_byte ^ ( data_byte * 32 );
}
72 PN 4237290A (April 1996)

APPENDIX C
FLAGS
Table C.1 Flags
Condition Flags Definitive Flags Suspect Flags* Other Flags
Normal WBC Pop Leukopenia Blasts Edited data

Abnormal WBC Pop Leukocytosis Imm Grans/Bands1 PRELIMINARY REPORT
Normal RBC Pop Neutropenia % Imm Grans/Bands2 COLLATE FAILED
Abnormal RBC Pop Neutropenia # Variant Lymphs
Normal PLT Pop Neutrophilia % Review Slide
Abnormal PLT Pop Neutrophilia # NRBCs
Verify Retic Lymphopenia % Dimorphic RBC Pop
Abnormal Subset Lymphopenia # Micro RBCs/
Lymphocytosis % RBC Fragments
Lymphocytosis # RBC Agglutination
Monocytosis % Platelet Clumps
Monocytosis # Giant Platelets
Eosinophilia % Inconsistent Diff CD
Eosinophilia # Subset Pop Shift
Basophilia % Unlysed RBCs
Basophilia #
Anemia
1+ Anisocytosis
2+ Anisocytosis
3+ Anisocytosis
1+ Microcytosis
2+ Microcytosis
3+ Microcytosis
1+ Macrocytosis
2+ Macrocytosis
3+ Macrocytosis
1+ Hypochromia
2+ Hypochromia
3+ Hypochromia
1+ Poikilocytosis
2+ Poikilocytosis
3+ Poikilocytosis
Erythrocytosis
Pancytopenia
Thrombocytopenia
Thrombocytosis
Small Platelets
Large Platelets
* Enabled by installing Suspect Flag XMIT PROM available from Customer Service.
PN 4237290A (April 1996) 73

FLAGS
74 PN 4237290A (April 1996)

APPENDIX D
Graphic Reconstruction
D.1 DF1 Valleys

The five valley fields are used to disect the DF1 scatterplot. Each valley field
is an ASCII representation of a value from 0 to 255. The values are used as
follows:
255 (VAL1,255) (VAL2,255)
VAL3 (0,VAL3) (VAL1, VAL3)
VAL4 (VAL1 VAL4) (VAL2,VAL4)
VAL5 (0, VAL5) (VAL1, VAL5)
0 VAL1 VAL2 255
Figure D.1
D.2 Diff Scatterplot

The DIFF scatterplot data consist of 4096 bytes of hexidecimal data. The
scatterplot hexidecimal data can only have values in the range of 00H to 0FH.
Each of the 4096 bytes of hexidecimal data is converted to 4096 bytes of
ASCII data before being transmitted to the host.
PN 4237290A (April 1996) 75

Table D.1 Hex to ASCII Conversion
HEX ASCII HEX ASCII

DATA DATA DATA DATA
00H 30H 08H 38H
01H 31H 09H 39H
02H 32H 0AH 41H
03H 33H 0BH 42H
04H 34H 0CH 43H
05H 35H 0DH 44H
06H 36H 0EH 45H
07H 37H 0FH 46H
To convert the transmitted 4096 ASCII bytes back to a hexidecimal array just
reverse the process by converting each ASCII byte to a hexidecimal byte.
(ex. 31H => 01H).
The 4096 hexidecimal bytes should be viewed as a two dimensional array of

64 bytes X 64 bytes. As previously stated each byte can only have a value
between 00H and 0FH.
The scatterplot is constructed by mapping each hexidecimal byte of the

scatterplot array to a colored 4 X 4 pixel pattern as illustrated in figure C.2.
Value: 00H 01H 02H - 04H 05H - 0AH 0BH - 0FH

Color: NONE BLUE GREEN RED YELLOW
Figure D.2A
The 4 X 4 pixel pattern is mapped into a 256 X 256 pixel graph as depicted in
Figure D.3.
255
(00,63) (01,63) (02,63) ... (63,63)
Y 251 . . . . .
. . . . .
P . . . . .
i
x 12 (00,02) (00,02) (02,02) ... (63,02)
e
l 8 (00,01) (01,01) (02,01) ... (63,01)
4
(00,00) (01,00) (02,00) ... (63,00)
0 0 4 8 12 251 255
X Pixel
Note: [][] (brackets) indicate index into scatterplot 64 X 64 array.
76 PN 4237290A (April 1996)

Histograms
D.3 Histograms
The histograms are transmitted as 512 ASCII bytes (256 bytes for PLT)
representing a 256 byte (128 byte for PLT) Hex array. It takes two ASCII
bytes to represent one Hex byte.
Each Hex byte represents a scaled count for each channel 0 - 255 (14 - 142 for
PLT). The histograms can be constructed by plotting the count for each
channel then drawing a line between plots.
For plotting the count is the y-axis and the channel is the x-axis.
y

C
O
U
N
T

x
CHANNEL
Figure D.3
PN 4237290A (April 1996) 77

78 PN 4237290A (April 1996)

APPENDIX E
RETICS GRAPHICS RECONSTRUCTION
E.1 DF5
255 (LLS22, 255) (LLS3, 255)
/
/
/
/
/
/
/
/
/
/
/
/
/
/
/
(255, DC) DC (0, DC) /
(LLS1, DC) (LLS3,DC)
0
255 0 LLS3
Figure E.1
E.2 DF6
255 (OP3, 255)
(255, DC) DC (0, DC) (OP3,

DC)
0
255 0 OP3
Figure E.2
PN 4237290A (April 1996) 79

RETICS GRAPHICS RECONSTRUCTION
80 PN 4237290A (April 1996)

APPENDIX F
CD4 and CD8 Graphics Reconstruction
F.1 CD4
255
VAL3
/ (VAL3, 255)
/
/
/
/
/
/
/
/
/
/
VAL4 (0,VAL4) /
(VAL2, VAL4)
(VAL1, VAL4)
0 (VAL1, 0)
0 VAL1 VAL2 255
Figure F.1
F.2 CD8
255 VAL3
/ (VAL3, 255)
/
/
/
/
/
/
/
/
/
/
VAL4 (0,VAL4) /
(VAL2, VAL4)
(VAL1, VAL4)
0 (VAL1, 0)
0 VAL2 VAL1 255
Figure F.2
PN 4237290A (April 1996) 81

CD4 and CD8 Graphics Reconstruction
82 PN 4237290A (April 1996)

APPENDIX G
1G1+Retics+Subsets Transmission Format Example of Collated
CBC/Diff/Retics/CD4/CD8
000000 0D 0A 0D 0A 0D 0A 0D 0A 0D 0A 0D 0A 2D 2D 2D 2D ············----
000010 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 0D 0A 11 30 41 0D ----------···0A·
000020 0A 44 41 54 45 20 30 33 2F 31 39 2F 39 36 20 0D ·DATE 03/19/96 ·
000030 0A 54 49 4D 45 20 31 35 3A 34 32 3A 32 30 20 20 ·TIME 15:42:20
000040 20 20 0D 0A 49 44 31 20 38 39 32 35 37 32 33 30 ··ID1 89257230
000050 34 34 37 00 00 00 00 00 0D 0A 49 44 32 20 54 68 447·······ID2 Th
000060 69 73 49 73 49 64 32 54 68 69 73 49 73 49 0D 0A isIsId2ThisIsI··
000070 43 41 53 53 2F 50 4F 53 20 30 30 30 32 2F 30 37 CASS/POS 0002/07
000080 20 0D 0A 53 45 51 55 45 4E 43 45 20 31 32 33 34 ··SEQUENCE 1234
000090 35 36 20 0D 0A 31 45 43 2F 50 20 20 30 30 30 32 56 ··1EC/P 0002
0000A0 30 37 20 0D 0A 49 44 31 53 54 41 54 55 53 20 50 07 ··ID1STATUS P
0000B0 20 0D 0A 43 2F 50 53 54 41 54 55 53 20 50 20 0D ··C/PSTATUS P ·
0000C0 0A 57 4C 53 54 41 54 55 53 20 47 4F 4F 44 20 20 ·WLSTATUS GOOD
0000D0 20 20 20 0D 0A 11 30 41 0D 0A 57 42 43 20 20 20 ···0A··WBC
0000E0 20 34 2E 39 00 20 20 20 0D 0A 52 42 43 20 20 20 4.9· ··RBC
0000F0 34 2E 34 38 00 20 20 20 0D 0A 48 47 42 20 20 20 4.48· ··HGB
000100 31 33 2E 34 00 20 20 20 0D 0A 48 43 54 20 20 20 13.4· ··HCT
000110 34 30 2E 30 00 20 20 20 0D 0A 4D 43 56 20 20 20 40.0· ··MCV
000120 38 39 2E 32 00 20 20 20 0D 0A 4D 43 48 20 20 20 89.2· ··MCH
000130 32 39 2E 39 00 20 20 20 0D 0A 4D 43 48 43 20 20 29.9· ··MCHC
000140 33 33 2E 35 00 20 20 20 0D 0A 52 44 57 20 20 20 33.5· ··RDW
000150 31 32 2E 36 00 20 20 20 0D 0A 50 4C 54 20 20 20 12.6· ··PLT
000160 20 32 30 32 00 20 20 20 0D 0A 4D 50 56 20 20 20 202· ··MPV
000170 20 38 2E 39 00 20 20 20 0D 0A 11 30 35 0D 0A 4C 8.9· ···05··L
000180 59 23 20 20 20 20 32 2E 36 00 20 20 20 0D 0A 4D Y# 2.6· ··M
000190 4F 23 20 20 20 20 30 2E 37 00 20 20 20 0D 0A 4E O# 0.7· ··N
0001A0 45 23 20 20 20 20 30 2E 39 00 20 20 4C 0D 0A 45 E# 0.9· L··E
0001B0 4F 23 20 20 20 20 30 2E 34 00 20 20 20 0D 0A 42 O# 0.4· ··B
0001C0 41 23 20 20 20 20 30 2E 33 00 20 20 48 0D 0A 11 A# 0.3· H···
0001D0 30 35 0D 0A 4C 59 25 20 20 20 35 32 2E 34 00 20 05··LY% 52.4·
0001E0 20 20 0D 0A 4D 4F 25 20 20 20 31 34 2E 30 00 20 ··MO% 14.0·
0001F0 20 48 0D 0A 4E 45 25 20 20 20 31 38 2E 35 00 20 H··NE% 18.5·
000200 20 4C 0D 0A 45 4F 25 20 20 20 20 38 2E 32 00 20 L··EO% 8.2·
000210 20 20 0D 0A 42 41 25 20 20 20 20 36 2E 39 00 20 ··BA% 6.9·
000220 20 48 0D 0A 11 30 31 0D 0A 63 6F 6D 6D 65 6E 74 H···01··comment
000230 73 39 30 31 32 33 34 35 36 37 38 39 30 31 32 33 s901234567890123
000240 34 35 36 37 38 39 30 31 32 33 34 35 36 37 38 39 4567890123456789
000250 30 31 32 33 34 35 36 37 38 39 30 31 32 33 34 35 0123456789012345
000260 36 37 38 39 30 31 32 33 34 0D 0A 11 30 38 0D 0A 678901234···08··
000270 41 62 6E 6F 72 6D 61 6C 20 57 42 43 20 50 6F 70 Abnormal WBC Pop
000280 0D 0A 4E 65 75 74 72 6F 70 65 6E 69 61 20 25 0D ··Neutropenia %·
000290 0A 4E 65 75 74 72 6F 70 65 6E 69 61 20 23 0D 0A ·Neutropenia #··
0002A0 4D 6F 6E 6F 63 79 74 6F 73 69 73 20 25 0D 0A 42 Monocytosis %··B
0002B0 61 73 6F 70 68 69 6C 69 61 20 25 0D 0A 42 61 73 asophilia %··Bas
0002C0 6F 70 68 69 6C 69 61 20 23 0D 0A 4E 6F 72 6D 61 ophilia #··Norma
0002D0 6C 20 52 42 43 20 50 6F 70 0D 0A 4E 6F 72 6D 61 l RBC Pop··Norma
0002E0 6C 20 50 4C 54 20 50 6F 70 0D 0A 11 30 39 0D 0A l PLT Pop···09··
0002F0 42 49 52 54 48 20 30 31 2F 32 36 2F 31 39 35 31 BIRTH 01/26/1951
000300 20 0D 0A 55 46 31 20 75 73 65 72 66 69 65 6C 64 ··UF1 userfield
000310 31 75 73 65 72 66 69 20 0D 0A 55 46 32 20 75 73 1userfi ··UF2 us
000320 65 72 66 69 65 6C 64 31 75 73 65 72 66 69 20 0D erfield1userfi ·
000330 0A 55 46 33 20 75 73 65 72 66 69 65 6C 64 31 75 ·UF3 userfield1u
000340 73 65 72 66 69 20 0D 0A 53 45 58 20 20 20 0D 0A serfi ··SEX ··
000350 4C 4F 43 41 54 49 4F 4E 20 6C 6F 63 61 74 69 6F LOCATION locatio
000360 6E 6C 6F 63 61 74 69 6F 6E 20 0D 0A 50 48 59 53 nlocation ··PHYS
000370 49 43 49 41 4E 20 70 68 79 73 69 63 69 61 6E 70 ICIAN physicianp
000380 68 79 73 69 63 69 61 6E 70 68 79 73 20 0D 0A 55 hysicianphys ··U
000390 44 41 54 45 20 30 33 2F 31 39 2F 39 36 20 0D 0A DATE 03/19/96 ··
0003A0 55 54 49 4D 45 20 31 35 3A 31 34 20 0D 0A 11 30 UTIME 15:14 ···0
0003B0 36 0D 0A 52 45 54 44 41 54 45 20 30 33 2F 31 39 6··RETDATE 03/19
0003C0 2F 39 36 20 0D 0A 52 45 54 54 49 4D 45 20 31 35 /96 ··RETTIME 15
0003D0 3A 33 34 3A 33 31 20 20 20 20 0D 0A 52 49 44 20 :34:31 ··RID
0003E0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0003F0 0D 0A 52 43 41 53 53 2F 50 4F 53 20 53 30 30 30 ··RCASS/POS S000
000400 32 2F 30 38 20 0D 0A 52 45 54 25 20 20 32 2E 30 2/08 ··RET% 2.0
000410 38 00 20 20 20 0D 0A 52 45 54 23 20 2E 30 39 33 8· ··RET# .093
000420 30 00 20 20 20 0D 0A 11 30 36 0D 0A 43 44 34 44 0· ···06··CD4D
000430 41 54 45 20 30 33 2F 31 39 2F 39 36 20 0D 0A 43 ATE 03/19/96 ··C
000440 44 34 54 49 4D 45 20 31 35 3A 34 35 3A 32 35 20 D4TIME 15:45:25
000450 20 20 20 0D 0A 43 44 34 49 44 20 38 39 32 35 38 ··CD4ID 89258
000460 39 36 32 35 35 39 20 20 20 20 20 0D 0A 43 44 34 962559 ··CD4
000470 43 41 53 53 2F 50 4F 53 20 30 30 30 32 2F 30 39 CASS/POS 0002/09
000480 20 0D 0A 43 44 34 25 20 20 33 30 2E 32 00 20 20 ··CD4% 30.2·
000490 20 0D 0A 43 44 34 23 20 20 31 30 35 36 00 20 20 ··CD4# 1056·
0004A0 20 0D 0A 11 30 37 0D 0A 43 44 38 44 41 54 45 20 ···07··CD8DATE
0004B0 30 33 2F 31 39 2F 39 36 20 0D 0A 43 44 38 54 49 03/19/96 ··CD8TI
0004C0 4D 45 20 31 35 3A 34 37 3A 35 35 20 20 20 20 0D ME 15:47:55 ·
0004D0 0A 43 44 38 49 44 20 38 39 32 35 38 39 36 32 35 ·CD8ID 892589625
0004E0 36 31 20 20 20 20 20 0D 0A 43 44 38 43 41 53 53 61 ··CD8CASS
0004F0 2F 50 4F 53 20 30 30 30 32 2F 30 34 20 0D 0A 43 /POS 0002/04 ··C
000500 44 38 25 20 20 20 38 2E 34 00 20 20 4C 0D 0A 43 D8% 8.4· L··C
PN 4237290A (April 1996) 83

1G1+Retics+Subsets Transmission Format Example of Collated CBC/Diff/Retics/CD4/CD8
000510 44 38 23 20 20 20 32 39 35 00 20 20 20 0D 0A 34 D8# 295· ··4

000520 3A 38 20 20 20 33 2E 35 39 00 20 20 20 0D 0A 11 :8 3.59· ···
000530 0D 0A 0D 0A 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D ····------------
000540 2D 2D 0D 0A 20 20 20 20 20 20 20 20 20 20 20 20 --··
000550 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
000560 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
000570 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
000580 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
000590 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0005A0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0005B0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0005C0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0005D0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0005E0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0005F0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
84 PN 4237290A (April 1996)

APPENDIX H
3A TRANSMISSION FORMAT EXAMPLE OFCOLLATED
CBC/DIFF/RETICS/CD4/CD8
000010 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 0D 0A 53 30 35 0D ----------··S05·
000020 0A 43 42 43 0D 0A 44 49 46 46 0D 0A 52 45 54 49 ·CBC··DIFF··RETI
000030 43 0D 0A 43 44 34 0D 0A 43 44 38 0D 0A 54 43 42 C··CD4··CD8··TCB
000040 43 0D 0A 30 37 0D 0A 47 30 31 0D 0A 30 38 0D 0A C··07··G01··08··
000050 44 41 54 45 20 31 31 2F 32 32 2F 39 35 0D 0A 54 DATE 11/22/95··T
000060 49 4D 45 20 31 31 3A 31 32 3A 35 39 0D 0A 49 44 IME 11:12:59··ID
000070 31 20 38 39 32 35 37 32 33 30 31 38 37 0D 0A 50 1 89257230187··P
000080 49 44 31 20 38 39 32 35 37 32 33 30 31 38 37 0D ID1 89257230187·
000090 0A 43 41 53 53 50 4F 53 20 50 30 30 30 32 30 34 ·CASSPOS P000204
0000A0 0D 0A 50 43 41 53 53 50 4F 53 20 30 30 30 32 30 ··PCASSPOS 00020
0000B0 34 0D 0A 49 44 31 53 54 41 54 55 53 20 50 0D 0A 4··ID1STATUS P··
0000C0 43 2F 50 53 54 41 54 55 53 20 50 0D 0A 47 30 32 C/PSTATUS P··G02
0000D0 0D 0A 30 41 0D 0A 57 42 43 20 20 20 37 2E 32 20 ··0A··WBC 7.2
0000E0 20 20 20 0D 0A 52 42 43 20 20 34 2E 36 35 20 20 ··RBC 4.65
0000F0 20 20 0D 0A 48 47 42 20 20 31 34 2E 38 20 20 20 ··HGB 14.8
000100 20 0D 0A 48 43 54 20 20 34 33 2E 37 20 20 20 20 ··HCT 43.7
000110 0D 0A 4D 43 56 20 20 39 33 2E 39 20 20 20 20 0D ··MCV 93.9 ·
000120 0A 4D 43 48 20 20 33 31 2E 38 20 20 20 20 0D 0A ·MCH 31.8 ··
000130 4D 43 48 43 20 20 33 33 2E 38 20 20 20 20 0D 0A MCHC 33.8 ··
000140 52 44 57 20 20 31 31 2E 37 20 20 20 20 0D 0A 50 RDW 11.7 ··P
000150 4C 54 20 20 20 32 37 30 20 20 20 20 0D 0A 4D 50 LT 270 ··MP
000160 56 20 20 20 38 2E 32 20 20 20 20 0D 0A 47 30 35 V 8.2 ··G05
000170 0D 0A 30 31 0D 0A 43 4F 4D 4D 45 4E 54 20 43 4F ··01··COMMENT CO
000180 4D 4D 45 4E 54 53 39 30 31 32 33 34 35 36 37 38 MMENTS9012345678
000190 39 30 31 32 33 34 35 36 37 38 39 30 31 32 33 34 9012345678901234
0001A0 35 36 37 38 39 30 31 32 33 34 35 36 37 38 39 30 5678901234567890
0001B0 31 32 33 34 35 36 37 38 39 30 31 32 33 34 0D 0A 12345678901234··
0001C0 47 30 38 0D 0A 30 33 0D 0A 43 4F 4E 44 49 54 49 G08··03··CONDITI
0001D0 4F 4E 20 4E 6F 72 6D 61 6C 20 57 42 43 20 50 6F ON Normal WBC Po
0001E0 70 0D 0A 43 4F 4E 44 49 54 49 4F 4E 20 4E 6F 72 p··CONDITION Nor
0001F0 6D 61 6C 20 52 42 43 20 50 6F 70 0D 0A 43 4F 4E mal RBC Pop··CON
000200 44 49 54 49 4F 4E 20 4E 6F 72 6D 61 6C 20 50 4C DITION Normal PL
000210 54 20 50 6F 70 0D 0A 47 30 41 0D 0A 30 43 0D 0A T Pop··G0A··0C··
000220 42 49 52 54 48 20 30 31 2F 30 31 2F 31 39 39 35 BIRTH 01/01/1995
000230 0D 0A 55 46 31 20 55 46 31 34 35 36 37 38 39 30 ··UF1 UF14567890
000240 31 32 33 34 35 36 0D 0A 55 46 32 20 55 46 32 34 123456··UF2 UF24
000250 35 36 37 38 39 30 31 32 33 34 35 36 0D 0A 55 46 567890123456··UF
000260 33 20 55 46 33 34 35 36 37 38 39 30 31 32 33 34 3 UF345678901234
000270 35 36 0D 0A 53 45 58 20 4D 0D 0A 4C 4F 43 41 54 56··SEX M··LOCAT
000280 49 4F 4E 20 4C 4F 43 34 35 36 37 38 39 30 31 32 ION LOC456789012
000290 33 34 35 36 0D 0A 50 48 59 53 49 43 49 41 4E 20 3456··PHYSICIAN
0002A0 50 48 59 34 35 36 37 38 39 30 31 32 33 34 35 36 PHY4567890123456
0002B0 37 38 0D 0A 45 44 20 31 31 2F 32 32 2F 39 35 0D 78··ED 11/22/95·
0002C0 0A 45 54 20 31 30 3A 35 37 0D 0A 49 44 32 20 49 ·ET 10:57··ID2 I
0002D0 44 23 32 35 36 37 38 39 30 31 32 33 34 35 36 0D D#2567890123456·
0002E0 0A 53 45 51 55 45 4E 43 45 20 31 32 33 34 35 36 ·SEQUENCE 123456
0002F0 0D 0A 50 46 20 36 0D 0A 47 30 45 0D 0A 30 31 0D ··PF 6··G0E··01·
000300 0A 52 42 43 48 20 30 30 30 30 30 30 30 30 30 30 ·RBCH 0000000000
000310 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000320 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000330 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000340 30 30 30 31 30 31 30 32 30 33 30 34 30 35 30 37 0001010203040507
000350 30 41 30 45 31 31 31 36 31 42 32 32 32 41 33 31 0A0E11161B222A31
000360 33 37 33 42 34 30 34 36 34 42 34 46 34 46 34 46 373B40464B4F4F4F
000370 34 46 34 45 34 44 34 38 34 33 33 45 33 38 33 31 4F4E4D48433E3831
000380 32 43 32 38 32 34 32 31 31 44 31 38 31 34 31 31 2C2824211D181411
000390 30 45 30 44 30 42 30 41 30 39 30 38 30 37 30 37 0E0D0B0A09080707
0003A0 30 37 30 36 30 36 30 35 30 35 30 34 30 34 30 34 0706060505040404
0003B0 30 35 30 35 30 35 30 34 30 34 30 35 30 35 30 35 0505050404050505
0003C0 30 35 30 35 30 35 30 35 30 35 30 35 30 35 30 35 0505050505050505
0003D0 30 35 30 35 30 36 30 36 30 36 30 36 30 36 30 35 0505060606060605
0003E0 30 35 30 35 30 35 30 35 30 35 30 34 30 34 30 35 0505050505040405
0003F0 30 34 30 34 30 34 30 34 30 34 30 34 30 34 30 34 0404040404040404
000400 30 33 30 33 30 32 30 32 30 32 30 32 30 33 30 32 0303020202020302
000410 30 32 30 32 30 32 30 32 30 32 30 32 30 32 30 32 0202020202020202
000420 30 32 30 32 30 31 30 31 30 31 30 31 30 31 30 31 0202010101010101
000430 30 31 30 31 30 30 30 30 30 30 30 31 30 31 30 31 0101000000010101
000440 30 31 30 31 30 31 30 30 30 30 30 30 30 30 30 30 0101010000000000
000450 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000460 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000470 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000480 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000490 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0004A0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0004B0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0004C0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0004D0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0004E0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0004F0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000500 30 30 30 30 30 30 0D 0A 47 30 46 0D 0A 30 32 0D 000000··G0F··02·
000510 0A 50 4C 54 48 20 30 32 30 32 30 42 30 42 30 45 ·PLTH 02020B0B0E
000520 30 45 31 32 31 32 31 37 31 37 31 42 31 42 32 30 0E121217171B1B20
PN 4237290A (April 1996) 85

3A TRANSMISSION FORMAT EXAMPLE OFCOLLATED CBC/DIFF/RETICS/CD4/CD8
000530 32 30 32 34 32 34 32 36 32 36 32 38 32 38 32 39 2024242626282829
000540 32 39 32 41 32 41 32 44 32 44 32 45 32 45 32 45 292A2A2D2D2E2E2E
000550 32 45 32 44 32 44 32 43 32 43 32 41 32 41 32 37 2E2D2D2C2C2A2A27
000560 32 37 32 34 32 34 32 32 32 32 31 46 31 46 31 45 27242422221F1F1E
000570 31 45 31 44 31 44 31 44 31 44 31 43 31 43 31 42 1E1D1D1D1D1C1C1B
000580 31 42 31 39 31 39 31 36 31 36 31 34 31 34 31 32 1B19191616141412
000590 31 32 31 31 31 31 30 46 30 46 30 44 30 44 30 43 1211110F0F0D0D0C
0005A0 30 43 30 42 30 42 30 41 30 41 30 39 30 39 30 39 0C0B0B0A0A090909
0005B0 30 39 30 39 30 39 30 39 30 39 30 39 30 39 30 38 0909090909090908
0005C0 30 38 30 37 30 37 30 36 30 36 30 36 30 36 30 36 0807070606060606
0005D0 30 36 30 36 30 36 30 35 30 35 30 34 30 34 30 34 0606060505040404
0005E0 30 34 30 33 30 33 30 33 30 33 30 32 30 32 30 32 0403030303020202
0005F0 30 32 30 32 30 32 30 31 30 31 30 31 30 31 30 31 0202020101010101
000600 30 31 30 31 30 31 30 31 30 31 30 30 30 30 30 30 0101010101000000
000610 30 30 30 30 30 30 0D 0A 50 4C 54 46 20 30 30 30 000000··PLTF 000
000620 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000630 30 30 30 30 30 30 31 30 31 30 34 30 34 30 37 30 0000001010404070
000640 37 30 41 30 41 30 46 30 46 31 34 31 34 31 39 31 70A0A0F0F1414191
000650 39 31 45 31 45 32 32 32 32 32 36 32 36 32 41 32 91E1E222226262A2
000660 41 32 43 32 43 32 44 32 44 32 46 32 46 32 46 32 A2C2C2D2D2F2F2F2
000670 46 32 46 32 46 32 45 32 45 32 44 32 44 32 43 32 F2F2F2E2E2D2D2C2
000680 43 32 41 32 41 32 38 32 38 32 36 32 36 32 35 32 C2A2A28282626252
000690 35 32 32 32 32 32 30 32 30 31 46 31 46 31 44 31 5222220201F1F1D1
0006A0 44 31 42 31 42 31 39 31 39 31 38 31 38 31 36 31 D1B1B19191818161
0006B0 36 31 34 31 34 31 33 31 33 31 31 31 31 31 30 31 6141413131111101
0006C0 30 30 46 30 46 30 45 30 45 30 43 30 43 30 43 30 00F0F0E0E0C0C0C0
0006D0 43 30 42 30 42 30 41 30 41 30 39 30 39 30 38 30 C0B0B0A0A0909080
0006E0 38 30 37 30 37 30 37 30 37 30 37 30 37 30 36 30 8070707070707060
0006F0 36 30 35 30 35 30 34 30 34 30 34 30 34 30 34 30 6050504040404040
000700 34 30 34 30 34 30 34 30 34 30 33 30 33 30 33 30 4040404040303030
000710 33 30 32 30 32 30 32 30 32 30 32 30 32 30 32 30 3020202020202020
000720 32 30 32 30 32 30 32 30 32 30 31 30 31 30 31 30 2020202020101010
000730 31 30 31 30 31 30 31 30 31 30 31 30 31 30 30 30 1010101010101000
000740 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000750 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000760 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000770 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000780 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000790 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0007A0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0007B0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0007C0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0007D0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0007E0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0007F0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000800 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000810 30 30 30 30 30 30 30 30 30 30 30 30 30 0D 0A 54 0000000000000··T
000820 44 49 46 46 0D 0A 30 36 0D 0A 47 30 31 0D 0A 30 DIFF··06··G01··0
000830 38 0D 0A 44 41 54 45 20 31 31 2F 32 32 2F 39 35 8··DATE 11/22/95
000840 0D 0A 54 49 4D 45 20 31 31 3A 31 32 3A 35 39 0D ··TIME 11:12:59·
000850 0A 49 44 31 20 38 39 32 35 37 32 33 30 31 38 37 ·ID1 89257230187
000860 0D 0A 50 49 44 31 20 38 39 32 35 37 32 33 30 31 ··PID1 892572301
000870 38 37 0D 0A 43 41 53 53 50 4F 53 20 50 30 30 30 87··CASSPOS P000
000880 32 30 34 0D 0A 50 43 41 53 53 50 4F 53 20 30 30 204··PCASSPOS 00
000890 30 32 30 34 0D 0A 49 44 31 53 54 41 54 55 53 20 0204··ID1STATUS
0008A0 50 0D 0A 43 2F 50 53 54 41 54 55 53 20 50 0D 0A P··C/PSTATUS P··
0008B0 47 30 33 0D 0A 30 35 0D 0A 4C 59 23 20 20 20 32 G03··05··LY# 2
0008C0 2E 35 20 20 20 20 0D 0A 4D 4F 23 20 20 20 30 2E .5 ··MO# 0.
0008D0 37 20 20 20 20 0D 0A 4E 45 23 20 20 20 33 2E 36 7 ··NE# 3.6
0008E0 20 20 20 20 0D 0A 45 4F 23 20 20 20 30 2E 34 20 ··EO# 0.4
0008F0 20 20 20 0D 0A 42 41 23 20 20 20 30 2E 30 20 20 ··BA# 0.0
000900 20 20 0D 0A 47 30 34 0D 0A 30 35 0D 0A 4C 59 25 ··G04··05··LY%
000910 20 20 33 34 2E 31 20 20 20 20 0D 0A 4D 4F 25 20 34.1 ··MO%
000920 20 20 39 2E 31 20 20 20 20 0D 0A 4E 45 25 20 20 9.1 ··NE%
000930 35 31 2E 36 20 20 20 20 0D 0A 45 4F 25 20 20 20 51.6 ··EO%
000940 35 2E 32 20 20 20 20 0D 0A 42 41 25 20 20 20 30 5.2 ··BA% 0
000950 2E 30 20 20 20 20 0D 0A 47 30 42 0D 0A 30 36 0D .0 ··G0B··06·
000960 0A 56 41 4C 31 20 31 31 37 0D 0A 56 41 4C 32 20 ·VAL1 117··VAL2
000970 31 37 34 0D 0A 56 41 4C 33 20 31 33 34 0D 0A 56 174··VAL3 134··V
000980 41 4C 34 20 30 38 38 0D 0A 56 41 4C 35 20 30 32 AL4 088··VAL5 02
000990 38 0D 0A 44 46 31 20 30 30 30 30 30 30 30 30 31 8··DF1 000000001
0009A0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0009B0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0009C0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0009D0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
0009E0 30 30 30 31 30 30 30 32 30 30 31 30 30 30 30 30 0001000200100000
0009F0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000A00 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000A10 30 30 30 30 30 30 30 30 30 30 30 30 30 31 32 31 0000000000000121
000A20 30 30 32 31 30 30 31 30 30 30 30 31 30 30 30 30 0021001000010000
000A30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000A40 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000A50 30 30 30 30 30 30 30 30 30 30 30 30 30 30 31 30 0000000000000010
000A60 31 30 30 30 30 30 31 30 31 30 30 31 31 30 30 31 1000001010011001
000A70 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000A80 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000A90 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 32 0000000000000002
000AA0 32 31 30 30 30 31 31 30 30 30 32 31 32 30 30 30 2100011000212000
000AB0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000AC0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
86 PN 4237290A (April 1996)

APPENDIX H
000AD0 30 30 30 30 30 30 30 30 30 30 30 31 30 30 30 31 0000000000010001
000AE0 30 30 31 31 30 31 30 34 33 30 32 34 32 30 30 30 0011010430242000
000AF0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000B00 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000B10 30 30 30 30 30 30 30 30 30 30 30 30 30 30 32 32 0000000000000022
000B20 34 31 32 31 30 30 32 32 32 33 32 33 32 31 32 32 4121002223232122
000B30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000B40 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000B50 30 30 30 30 30 30 30 30 30 30 30 30 30 30 31 30 0000000000000010
000B60 31 33 31 30 30 31 31 30 35 37 31 33 36 32 32 30 1310011057136220
000B70 30 31 31 31 30 30 30 30 30 30 30 30 30 30 30 30 0111000000000000
000B80 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000B90 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000BA0 31 32 32 30 30 33 31 31 35 39 36 33 36 33 32 31 1220031159636321
000BB0 31 30 31 30 31 30 30 30 30 30 30 30 30 30 30 30 1010100000000000
000BC0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000BD0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 33 31 0000000000000031
000BE0 31 33 32 31 30 30 31 31 36 38 33 34 34 32 32 31 1321001168344221
000BF0 30 31 31 30 30 30 30 30 30 31 30 30 30 30 30 30 0110000001000000
000C00 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000C10 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 31 0000000000000001
000C20 34 33 30 32 31 36 33 32 36 33 41 32 32 34 32 31 4302163263A22421
000C30 31 30 31 30 30 30 30 30 30 30 30 30 31 30 30 30 1010000000001000
000C40 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000C50 30 30 30 30 30 30 30 30 30 30 30 30 30 31 31 30 0000000000000110
000C60 32 30 31 31 30 31 32 33 41 38 37 41 36 35 32 30 20110123A87A6520
000C70 32 30 32 30 30 30 30 30 30 31 30 30 30 30 30 30 2020000001000000
000C80 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000C90 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 31 0000000000000001
000CA0 30 31 31 30 33 31 33 37 36 42 41 42 36 35 41 34 011031376BAB65A4
000CB0 36 30 31 30 30 30 30 30 30 30 30 30 30 30 30 30 6010000000000000
000CC0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000CD0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 31 32 0000000000000012
000CE0 32 30 30 30 30 33 34 36 42 42 42 41 42 41 39 42 20000346BBBABA9B
000CF0 39 33 30 32 31 31 30 30 30 30 30 30 30 30 30 30 9302110000000000
000D00 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000D10 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000D20 31 30 32 32 30 35 35 35 39 42 42 42 42 42 42 42 102205559BBBBBBB
000D30 34 39 37 30 31 30 30 31 30 31 30 30 30 30 30 30 4970100101000000
000D40 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000D50 30 30 30 30 30 30 30 30 30 30 30 31 30 30 30 30 0000000000010000
000D60 30 30 32 30 34 30 38 42 42 42 42 42 42 42 42 42 0020408BBBBBBBBB
000D70 39 35 34 34 33 31 31 30 30 30 30 31 30 30 30 30 9544311000010000
000D80 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000D90 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000DA0 30 30 32 31 31 32 32 37 42 42 42 42 42 42 42 42 00211227BBBBBBBB
000DB0 42 38 38 31 30 32 30 32 32 30 30 30 30 31 31 30 B881020220000110
000DC0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000DD0 30 30 30 30 30 30 30 30 30 30 30 31 30 30 30 30 0000000000010000
000DE0 30 30 31 30 31 32 32 42 39 42 42 42 42 42 42 42 0010122B9BBBBBBB
000DF0 42 42 41 32 30 30 32 31 31 30 31 30 30 32 32 30 BBA2002110100220
000E00 30 31 30 30 31 30 30 30 30 30 30 30 30 30 30 30 0100100000000000
000E10 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000E20 30 31 30 30 30 32 32 37 37 42 42 42 42 42 42 42 010002277BBBBBBB
000E30 42 42 38 36 35 32 35 34 31 32 33 31 32 32 30 31 BB86525412312201
000E40 31 30 30 30 31 30 30 30 30 30 30 30 30 31 30 30 1000100000000100
000E50 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000E60 30 30 31 30 30 32 33 34 42 42 42 42 42 42 42 42 00100234BBBBBBBB
000E70 42 38 36 35 35 30 30 32 30 33 31 31 31 36 33 33 B865500203111633
000E80 33 33 31 31 34 30 30 30 30 31 30 30 30 30 30 30 3311400001000000
000E90 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000EA0 30 30 30 30 30 30 31 34 42 42 42 42 42 42 42 42 00000014BBBBBBBB
000EB0 42 42 42 38 36 32 31 33 32 31 34 33 33 34 34 38 BBB8621321433448
000EC0 31 33 32 31 33 35 30 32 31 31 30 30 30 31 30 30 1321350211000100
000ED0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000EE0 30 30 30 30 30 30 33 37 39 35 42 42 42 42 42 42 0000003795BBBBBB
000EF0 42 38 39 37 35 33 31 32 34 32 33 32 33 41 36 37 B897531242323A67
000F00 35 31 39 35 35 33 30 32 30 31 30 30 30 30 30 30 5195530201000000
000F10 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000F20 30 30 30 30 30 30 30 33 34 35 42 38 42 42 42 42 0000000345B8BBBB
000F30 37 38 39 36 31 33 33 32 32 31 33 34 33 33 36 37 7896133221343367
000F40 35 42 36 36 32 33 34 31 31 32 33 30 32 30 30 30 5B66234112302000
000F50 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000F60 30 30 30 30 30 31 30 30 32 34 32 36 42 36 42 38 000001002426B6B8
000F70 42 38 34 38 31 31 30 32 30 33 32 31 36 39 39 42 B84811020321699B
000F80 42 42 42 35 36 33 34 32 30 32 30 30 31 30 30 32 BBB5634202001002
000F90 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
000FA0 30 30 30 30 30 30 30 30 31 30 31 31 35 42 42 36 0000000010115BB6
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PN 4237290A (April 1996) 87

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88 PN 4237290A (April 1996)

APPENDIX H
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PN 4237290A (April 1996) 89

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90 PN 4237290A (April 1996)

APPENDIX H
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PN 4237290A (April 1996) 91

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92 PN 4237290A (April 1996)

APPENDIX H
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PN 4237290A (April 1996) 93

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94 PN 4237290A (April 1996)

APPENDIX H
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PN 4237290A (April 1996) 95

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96 PN 4237290A (April 1996)

APPENDIX H
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PN 4237290A (April 1996) 97

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98 PN 4237290A (April 1996)

APPENDIX H
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PN 4237290A (April 1996) 99

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100 PN 4237290A (April 1996)

APPENDIX H
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PN 4237290A (April 1996) 101

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102 PN 4237290A (April 1996)

APPENDIX H
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PN 4237290A (April 1996) 103

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104 PN 4237290A (April 1996)

APPENDIX H
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PN 4237290A (April 1996) 105

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007B60 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
007B70 30 30 30 30 30 30 30 30 30 30 31 30 30 31 30 30 0000000000100100
007B80 30 30 30 31 30 30 30 32 30 30 30 30 30 30 30 30 0001000200000000
007B90 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
007BA0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
007BB0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
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007BE0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
007BF0 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
007C00 30 31 30 30 30 31 30 30 30 30 30 30 30 30 30 30 0100010000000000
007C10 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
007C20 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
007C30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
007C40 30 30 30 30 30 30 30 31 30 30 30 30 30 30 30 30 0000000100000000
007C50 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
007C60 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
007C70 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
007C80 30 30 30 31 30 30 31 30 30 30 30 30 30 30 30 30 0001001000000000
106 PN 4237290A (April 1996)

APPENDIX H
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0080A0 31 30 32 30 31 30 31 30 31 30 31 30 30 30 31 30 1020101010100010
0080B0 31 30 30 30 31 30 31 30 31 30 31 30 30 30 31 30 1000101010100010
0080C0 31 30 31 30 31 30 30 30 30 30 31 30 30 30 31 30 1010100000100010
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0080F0 30 30 31 30 31 30 31 30 32 30 32 30 32 30 34 30 0010101020202040
008100 36 30 36 30 36 30 41 30 38 30 39 30 43 30 43 31 606060A08090C0C1
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008170 42 34 45 34 39 34 45 34 45 34 45 34 45 34 43 34 B4E494E4E4E4E4C4
008180 37 33 45 34 44 34 34 33 38 33 36 32 42 33 33 32 73E4D4438362B332
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PN 4237290A (April 1996) 107

008240 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 30 0000000000000000
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0084D0 30 30 30 30 30 30 30 30 30 30 30 30 30 0D 0A 0D 0000000000000···
0084E0 0A 0D 0A 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D ···-------------
0084F0 2D 0D 0A 20 20 20 20 20 20 20 20 20 20 20 20 20 -··
108 PN 4237290A (April 1996)

APPENDIX I
3A TRANSMISSION FORMAT EXAMPLE OF 5C CONTROL
00000 00 00 0D 0A 0D 0A 0D 0A 0D 0A 0D 0A 0D 0A 2D 2D ..............--
00010 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 0D 0A 53 30 ------------..S0
00020 31 0D 0A 35 43 43 0D 0A 54 35 43 43 0D 0A 30 34 1..5CC..T5CC..04
00030 0D 0A 47 32 32 0D 0A 30 39 0D 0A 52 55 4E 23 20 ..G22..09..RUN#
00040 37 0D 0A 4C 4F 54 23 20 38 38 34 36 30 30 0D 0A 7..LOT# 884600..
00050 55 43 4E 41 4D 45 20 4E 6F 72 6D 61 6C 20 20 20 UCNAME Normal
00060 20 20 20 0D 0A 45 58 50 44 41 54 45 20 31 31 2F ..EXPDATE 11/
00070 31 39 2F 39 33 0D 0A 44 41 54 45 20 31 31 2F 31 19/93..DATE 11/1
00080 37 2F 39 33 0D 0A 54 49 4D 45 20 31 35 3A 31 34 7/93..TIME 15:14
00090 0D 0A 4F 50 45 52 41 54 4F 52 20 4F 50 52 0D 0A ..OPERATOR OPR..
000A0 49 51 41 50 20 31 32 33 34 35 2D 36 2D 37 38 2D IQAP 12345-6-78-
000B0 39 0D 0A 53 48 49 46 54 20 31 0D 0A 47 32 33 0D 9..SHIFT 1..G23.
000C0 0A 30 43 0D 0A 57 42 43 20 20 20 39 2E 35 20 20 .0C..WBC 9.5
000D0 0D 0A 52 42 43 20 20 35 2E 37 35 20 20 0D 0A 48 ..RBC 5.75 ..H
000E0 47 42 20 20 31 37 2E 31 20 20 0D 0A 48 43 54 20 GB 17.1 ..HCT
000F0 20 34 39 2E 36 20 20 0D 0A 4D 43 56 20 20 38 36 49.6 ..MCV 86
00100 2E 33 20 20 0D 0A 4D 43 48 20 20 32 39 2E 37 20 .3 ..MCH 29.7
00110 20 0D 0A 4D 43 48 43 20 20 33 34 2E 34 20 20 0D ..MCHC 34.4 .
00120 0A 52 44 57 20 20 31 33 2E 35 20 20 0D 0A 50 4C .RDW 13.5 ..PL
00130 54 20 20 20 32 33 31 20 20 0D 0A 50 43 54 20 30 T 231 ..PCT 0
00140 2E 32 34 32 20 20 0D 0A 4D 50 56 20 20 31 30 2E .242 ..MPV 10.
00150 35 20 20 0D 0A 50 44 57 20 20 31 34 2E 37 20 20 5 ..PDW 14.7
00160 0D 0A 47 32 34 0D 0A 30 35 0D 0A 4C 59 23 20 20 ..G24..05..LY#
00170 20 32 2E 36 20 20 0D 0A 4D 4F 23 20 20 20 30 2E 2.6 ..MO# 0.
00180 38 20 20 0D 0A 4E 45 23 20 20 20 35 2E 33 20 20 8 ..NE# 5.3
00190 0D 0A 45 4F 23 20 20 20 30 2E 38 20 20 0D 0A 42 ..EO# 0.8 ..B
001A0 41 23 20 20 20 30 2E 30 20 20 0D 0A 47 32 35 0D A# 0.0 ..G25.
001B0 0A 30 35 0D 0A 4C 59 25 20 20 32 36 2E 39 20 20 .05..LY% 26.9
001C0 0D 0A 4D 4F 25 20 20 20 38 2E 31 20 20 0D 0A 4E ..MO% 8.1 ..N
001D0 45 25 20 20 35 37 2E 30 20 20 0D 0A 45 4F 25 20 E% 57.0 ..EO%
001E0 20 20 37 2E 39 20 20 0D 0A 42 41 25 20 20 20 30 7.9 ..BA% 0
001F0 2E 31 20 20 0D 0A 0D 0A 0D 0A 2D 2D 2D 2D 2D 2D .1 ......------
00200 2D 2D 2D 2D 2D 2D 2D 2D 0D 0A 0A 0A 0A 0A 20 20 --------......
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00270 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
00280 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
00290 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
002A0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
002B0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
002C0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
002D0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
002E0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
002F0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
PN 4237290A (April 1996) 109

3A TRANSMISSION FORMAT EXAMPLE OF 5C CONTROL
110 PN 4237290A (April 1996)

APPENDIX J
3A TRANSMISSION FORMAT EXAMPLE OF RETIC CONTROL
00000 00 00 0D 0A 0D 0A 0D 0A 0D 0A 0D 0A 0D 0A 2D 2D ..............--
00020 31 0D 0A 52 45 54 49 43 43 0D 0A 54 52 45 54 49 1..RETICC..TRETI
00030 43 43 0D 0A 30 32 0D 0A 47 32 32 0D 0A 30 41 0D CC..02..G22..0A.
00040 0A 52 55 4E 23 20 34 0D 0A 4C 4F 54 23 20 34 35 .RUN# 4..LOT# 45
00050 30 31 32 0D 0A 55 43 4E 41 4D 45 20 4C 65 76 65 012..UCNAME Leve
00060 6C 20 49 49 20 20 20 20 0D 0A 45 58 50 44 41 54 l II ..EXPDAT
00070 45 20 31 32 2F 31 30 2F 39 33 0D 0A 44 41 54 45 E 12/10/93..DATE
00080 20 31 31 2F 31 31 2F 39 33 0D 0A 54 49 4D 45 20 11/11/93..TIME
00090 31 34 3A 35 35 0D 0A 4F 50 45 52 41 54 4F 52 20 14:55..OPERATOR
000A0 4F 50 52 0D 0A 49 51 41 50 20 34 35 36 37 38 2D OPR..IQAP 45678-
000B0 39 2D 30 31 2D 32 0D 0A 53 48 49 46 54 20 30 0D 9-01-2..SHIFT 0.
000C0 0A 52 52 42 43 20 20 34 2E 35 30 0D 0A 47 32 36 .RRBC 4.50..G26
000D0 0D 0A 30 35 0D 0A 52 45 54 25 20 20 31 2E 35 33 ..05..RET% 1.53
000E0 20 20 0D 0A 52 45 54 23 20 2E 30 36 39 31 20 20 ..RET# .0691
000F0 0D 0A 4D 52 56 20 31 30 39 2E 33 20 20 0D 0A 4D ..MRV 109.3 ..M
00100 49 20 20 20 30 2E 33 20 20 0D 0A 4F 54 48 45 52 I 0.3 ..OTHER
00110 20 4D 52 56 20 61 6E 64 20 4D 49 20 70 61 72 61 MRV and MI para
00120 6D 65 74 65 72 73 20 61 72 65 20 4E 6F 74 20 66 meters are Not f
00130 6F 72 20 44 69 61 67 6E 6F 73 74 69 63 20 55 73 or Diagnostic Us
00140 65 2E 0D 0A 0D 0A 0D 0A 2D 2D 2D 2D 2D 2D 2D 2D e.......--------
00150 2D 2D 2D 2D 2D 2D 0D 0A 0A 0A 0A 0A 20 20 20 20 ------......
00160 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
00170 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
00180 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
00190 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
001A0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
001B0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
001C0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
001D0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
001E0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
001F0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
PN 4237290A (April 1996) 111

3A TRANSMISSION FORMAT EXAMPLE OF RETIC CONTROL
112 PN 4237290A (April 1996)

APPENDIX K
3A TRANSMISSION FORMAT EXAMPLE OF LATEX CONTROL
00000 20 20 0D 0A 0D 0A 0D 0A 0D 0A 0D 0A 0D 0A 2D 2D ............--
00020 31 0D 0A 4C 41 54 45 58 43 0D 0A 54 4C 41 54 45 1..LATEXC..TLATE
00030 58 43 0D 0A 30 32 0D 0A 47 31 46 0D 0A 30 36 0D XC..02..G1F..06.
00040 0A 56 5F 4D 4E 20 20 32 38 2E 38 20 20 0D 0A 43 .V_MN 28.8 ..C
00050 5F 4D 4E 20 20 32 38 2E 39 20 20 0D 0A 53 5F 4D _MN 28.9 ..S_M
00060 4E 20 20 38 33 2E 37 20 4C 0D 0A 56 5F 43 56 20 N 83.7 L..V_CV
00070 20 31 32 2E 30 20 48 0D 0A 43 5F 43 56 20 20 31 12.0 H..C_CV 1
00080 32 2E 38 20 48 0D 0A 53 5F 43 56 20 20 20 37 2E 2.8 H..S_CV 7.
00090 39 20 20 0D 0A 47 32 32 0D 0A 30 33 0D 0A 44 41 9 ..G22..03..DA
000A0 54 45 20 31 31 2F 32 39 2F 39 33 0D 0A 54 49 4D TE 11/29/93..TIM
000B0 45 20 30 39 3A 31 38 0D 0A 49 51 41 50 20 31 32 E 09:18..IQAP 12
000C0 33 34 35 2D 36 2D 37 38 2D 39 0D 0A 0D 0A 0D 0A 345-6-78-9......
000D0 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 0D 0A --------------..
000E0 0A 0A 0A 0A 20 20 20 20 20 20 20 20 20 20 20 20 ....
000F0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
00100 1A .
PN 4237290A (April 1996) 113

3A TRANSMISSION FORMAT EXAMPLE OF LATEX CONTROL
114 PN 4237290A (April 1996)

APPENDIX L
3A TRANSMISSION FORMAT EXAMPLE OF CD4 CONTROL
000010 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 0D 0A 53 30 31 0D ----------··S01·
000020 0A 43 44 34 43 0D 0A 54 43 44 34 43 0D 0A 30 32 ·CD4C··TCD4C··02
000030 0D 0A 47 32 32 0D 0A 30 42 0D 0A 52 55 4E 23 20 ··G22··0B··RUN#
000040 31 0D 0A 4C 4F 54 23 20 32 33 34 35 36 37 0D 0A 1··LOT# 234567··
000050 55 43 4E 41 4D 45 20 43 44 34 20 4E 4F 52 4D 41 UCNAME CD4 NORMA
000060 4C 20 20 0D 0A 45 58 50 44 41 54 45 20 31 32 2F L ··EXPDATE 12/
000070 33 31 2F 39 35 0D 0A 44 41 54 45 20 31 31 2F 30 31/95··DATE 11/0
000080 37 2F 39 35 0D 0A 54 49 4D 45 20 31 30 3A 34 34 7/95··TIME 10:44
000090 3A 33 39 0D 0A 4F 50 45 52 41 54 4F 52 20 4F 2E :39··OPERATOR O.
0000A0 56 0D 0A 49 51 41 50 20 31 32 33 34 35 2D 36 2D V··IQAP 12345-6-
0000B0 37 38 2D 39 0D 0A 53 48 49 46 54 20 30 0D 0A 43 78-9··SHIFT 0··C
0000C0 41 53 53 50 4F 53 20 30 30 30 32 30 36 0D 0A 52 ASSPOS 000206··R
0000D0 57 42 43 20 20 20 36 2E 36 0D 0A 47 32 39 0D 0A WBC 6.6··G29··
0000E0 30 32 0D 0A 43 44 34 25 20 20 33 37 2E 37 20 20 02··CD4% 37.7
0000F0 0D 0A 43 44 34 23 20 20 32 34 38 37 20 20 0D 0A ··CD4# 2487 ··
000100 0D 0A 0D 0A 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D 2D ····------------
000110 2D 2D 0D 0A 20 20 20 20 20 20 20 20 20 20 20 20 --··
000120 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
000130 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
000140 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
000150 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
000160 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
000170 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
000180 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
000190 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0001A0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0001B0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0001C0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0001D0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0001E0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
0001F0 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20 20
PN 4237290A (April 1996) 115

3A TRANSMISSION FORMAT EXAMPLE OF CD4 CONTROL
116 PN 4237290A (April 1996)

TRADEMARKS
AccuComp, ACCUVETTE, ACCU-ZYME, AQUA-AD, AUTO-CAL, AUTO-CLONE, CARDS,

CASH, "CC" logo, CHANNELYZER, CHEMOTERGE, COMPLETE CELL ANALYSIS, COSINE,
COULTER, COULTER CHEMISTRY, COULTER CLENZ, COULTER CLONE, THE COULTER
COUNTDOWN, COULTER COUNTER, COULTER CURRENTS, COULTERAMA, Cyto-
Spheres, CYTO-COMP, CYTO-STAT, CYTO-TROL, C-ZYME, DACAL, DACOS, "DACOS"
logo, DART, DIFF3, DIFF3 50, DIFF4, DILU-PACK, E.A.SY. 1, EASY 88, EASY 2, EPICS,
FACULTY, FASTECS, Flow-Check, Flow-Count, Flow-Set, 5C, 4C, HEMO-CAL,
HEMOTERGE, HEMO-W, IsoFlow, ISOLYSE, ISOPET, ISOTERGE, ISOTON, LANGLEY
FORD, LANGLEY FORD INSTRUMENTS, LEASE-PAK, "LFI" logo, LYSE S, MDADS, MINI-
KEM, NANO-SIZER, OMNISORP, OptiChem, Retic-Cal, reticONE, Retic-STAT, S-CAL,
SafePrep, SOMACOUNT, SOMAFIX, SOMATON, STAIN RIGHT, SYSTEM II, tetraCHROME,
tetraONE, triCHROME, THROMBOCOUNTER, THROMBO-FUGE, U.V.-ZYME,
ZAP-OGLOBIN, ZAPONIN and ZETAFUGE are trademarks of Coulter Corporation.
4/17/96
Copyright © Coulter Corporation 1996
All Rights Reserved
Printed on Recycled Papar
COULTER CORPORATION
Miami, Florida 33196

Host Transmission Specifications Coulter STKS

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COULTER® STKS Analyzer

COULTER® STKS Analyzer with Reticulocyte Analysis

DMS 3A Host Transmission

PN 4237290A (April 1996)

HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS

WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:

WARNING - Might cause injury.

System integrity might be compromised and operational errors might occur:

Initial Issue, 04/96

PN 4237290A (April 1996) iii

iv PN 4237290A (April 1996)

1.1 DOCUMENT SCOPE AND PURPOSE, 1

1.2 INTENDED USE, 1

2.3 HARDWARE INTERFACE, 3

2.5 COMMUNICATION PARAMETERS, 4

3 DMS TO HOST COMMUNICATIONS, 9

3.2 1G1, 1G1+RETICS, AND 1G1+RETICS+SUBSETS FORMAT

PN 4237290A (April 1996) v

3.3 2A and 3A Format Protocols, 31

vi PN 4237290A (April 1996)

Control CD4 Parameters Group 41, 59

4 HOST TO DMS COMMUNICATIONS (HOST WORKLIST), 61

APPENDIX A: ASCII TABLES, 69

A.1 Bit ASCII Codes, 69

A.2 Valid Host Communications ASCII Codes, 69

B.1 CRC Algorithm, 71

B.2 CRC Example Written in ASM86, 72

B.3 CRC Example Written in C, 72

APPENDIX D: GRAPHIC RECONSTRUCTION, 75

D.1 DF1 Valleys, 75

D.2 Diff Scatterplot, 75

APPENDIX E: RETICS GRAPHICS RECONSTRUCTION, 79

APPENDIX F: CD4 AND CD8 GRAPHICS RECONSTRUCTION, 81

PN 4237290A (April 1996) vii

APPENDIX G: 1G1+RETICS+SUBSETS TRANSMISSION FORMAT

APPENDIX H: 3A TRANSMISSION FORMAT EXAMPLE OFCOLLATED

APPENDIX I: 3A TRANSMISSION FORMAT EXAMPLE OF 5C CONTROL, 109

APPENDIX J: 3A TRANSMISSION FORMAT EXAMPLE OF RETIC

APPENDIX K: 3A TRANSMISSION FORMAT EXAMPLE OF LATEX

APPENDIX L: 3A TRANSMISSION FORMAT EXAMPLE OF CD4

viii PN 4237290A (April 1996)

1.1 DOCUMENT SCOPE AND PURPOSE

1.2 INTENDED USE

This document will specify the DMS to host computer communication

PN 4237290A (April 1996) 1

2 PN 4237290A (April 1996)

High Level Protocol

2.3 HARDWARE INTERFACE

P3 Pin No. Signal Name Flow Direction

PN 4237290A (April 1996) 3

<-- CTS on (send some data)

2.5 COMMUNICATION PARAMETERS

4 PN 4237290A (April 1996)

Note: The slowest recommended baud rate is 2400 for non-graphic

PN 4237290A (April 1996) 5

Table 1. F6-GRAPHICS Options

1G1, 2A, 3A, MAXM (1G1 + Retics), 1G1+Retics+Subsets

Table 2 lists the compatibility options (format protocols) that are

Table 2. Compatibility Options

6 PN 4237290A (April 1996)