Scribd Logo
Upload

Welcome to Scribd!

  • 118 views

    114100071

    Uploaded by

    Johnmar Aquino
    This document provides instructions for using a reagent to quantify triglyceride levels in serum or plasma samples. The reagent uses an enzymatic method involving lipoprotein lipase, glycerol kinase, and glycerol-3-phosphate oxidase to produce a violet colored complex that can be measured at 546nm. The reagent is linear for triglyceride concentrations up to 1000mg/dL. Normal triglyceride ranges are provided as a guideline.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    114100071

    Uploaded by

    Johnmar Aquino
    118 views1 page
    This document provides instructions for using a reagent to quantify triglyceride levels in serum or plasma samples. The reagent uses an enzymatic method involving lipoprotein lipase, glycerol kinase, and glycerol-3-phosphate oxidase to produce a violet colored complex that can be measured at 546nm. The reagent is linear for triglyceride concentrations up to 1000mg/dL. Normal triglyceride ranges are provided as a guideline.

    Original Description:

    Agappe reagent insert 5

    Copyright

    © © All Rights Reserved

    Available Formats

    PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    This document provides instructions for using a reagent to quantify triglyceride levels in serum or plasma samples. The reagent uses an enzymatic method involving lipoprotein lipase, glycerol kinase, and glycerol-3-phosphate oxidase to produce a violet colored complex that can be measured at 546nm. The reagent is linear for triglyceride concentrations up to 1000mg/dL. Normal triglyceride ranges are provided as a guideline.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    118 views1 page

    114100071

    Uploaded by

    Johnmar Aquino
    This document provides instructions for using a reagent to quantify triglyceride levels in serum or plasma samples. The reagent uses an enzymatic method involving lipoprotein lipase, glycerol kinase, and glycerol-3-phosphate oxidase to produce a violet colored complex that can be measured at 546nm. The reagent is linear for triglyceride concentrations up to 1000mg/dL. Normal triglyceride ranges are provided as a guideline.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 1

    4x10 mL/ 5x25 mL/ 6x50 mL/ 5x100 mL

    TRIGLYCERIDES (S.L) 11410007/ 11410002/ 11410008/ 11410004

    INTENDED USE Mode of Reaction End Point


    This reagent is intended for in vitro quantitative determination of triglycerides in Slope of reaction Increasing
    serum or plasma.
    Wavelength I 546 nm (540-560 nm)
    - GPO-TOPS methodology
    - Linear up to 1000 mg/dL Wavelength II 630 nm
    - Contains LCF (Lipaemic clearing factor) which minimizes rerun Temperature 370C
    CLINICAL SIGNIFICANCE Standard Concentration 200 mg/dL
    Triglycerides are simple lipids, formed in the liver by glycerol & fatty acids. They are Linearity 1000 mg/dL
    transported by VLDL, LDL & constitute about 95% of fat, stored as source of energy Blank Reagent
    in the tissue & plasma.
    Incubation time 5 min
    Increased levels are found in hyperlipidemias, diabetes, nephrotic syndrome &
    hypothyroidism. Increased levels are risk factor for arteriosclerotic coronary disease, Sample volume 10 μL
    peripheral vascular disease, acute pancreatitis & hyperlipoproteinaemia. Decreased Reagent volume 1000 μL
    levels are found in malnutrition & hyperthyroidism.
    Cuvette 1 cm light path
    PRINCIPLE LABORATORY PROCEDURE
    Enzymatic determination of triglyceride is based on following reactions: Blank Standard Sample
    LPL Working Reagent 1000 μL 1000 μL
    TGL+H2O ------ > Glycerol + Fatty acid 1000 μL
    GK Standard - 10 μL -
    Glycerol + ATP ------ > Glycerol-3-phosphate + ADP Sample - - 10 μL
    0
    Mg++ Mix and incubate for 5 minutes at 37 C. Measure the change in absorbance of
    GPO standard and sample against reagent blank.
    Glycerol-3-phospahte+O2 ------- > Dihydroxyacetone phosphate +H2O2 CALCULATION
    POD Absorbance of Sample
    2H2O2+4-Aminoantipyrine+ TOPS ------- > Violet coloured complex Triglycerides Con. (mg/dL) = ------------------------------ x 200
    GPO = Glycereol-3-phosphate Oxidase. Absorbance of Standard
    LPL = Lipoprotein Lipase
    BIBLIOGRAPHY
    GK = Glycerol Kinase
    1. Schettler, G., Nussel, E.; Arav. Med 10, 25 (1975)
    REAGENT COMPOSITION 2. Jacobs, N.J. , VanDemark, P.J.; Arch, Biochem, Biophy. 88, 250 – 255 (1960)
    TRIGLYCERIDES REAGENT 4x10mL / 5x25mL / 6x50mL / 5x100mL
    Pipes –buffer (pH 7.00) 5 mmol/L
    TOPS 5.3 mmol/L
    Potassium ferrocynate 10 mmol/L
    Magnesium Salt 17 mmol/L
    4-Aminoantipyrine 0.9 mmol/L
    ATP 3.15mmol/L
    Lipoprotein Lipase > 1800 U/L
    Glycerol Kinase > 450 U/L
    Glycerol – 3- phosphate oxidase > 3500 U/L
    Peroxidase > 450 U/L
    TRIGLYCERIDES STANDARD 1 x 4 mL
    Triglycerides std.concentration 200 mg/dL
    STORAGE AND STABILITY
    The sealed reagents are stable up to the expiry date stated on the label, when stored
    at 2 - 80C.
    LINEARITY
    This reagent is linear up to 1000 mg/dL.
    If the concentration is greater than linearity (1000 mg/dL), dilute the sample with
    normal saline and repeat the assay. Multiply the result with dilution factor.
    NORMAL RANGE
    It is recommended that each laboratory establish its own reference values.
    The following value may be used as guide line.
    Male : 60-165 mg/dL
    Female : 40-140 mg/dL
    THE REAGENT IS READY TO USE
    PRECAUTION
    To avoid contamination, use clean laboratory wares.
    Avoid direct exposure of working reagent to light.
    SAMPLE
    Serum / plasma (free of haemolysis)
    GENERAL SYSTEM PARAMETER

    ADL/V.02/110314

    You might also like