Plane Wedge Knife

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MICROTOME KNIVES

3. Plane-Wedge Knife

 Description:
- The wedge shaped knife has more rigidity than plane-concave knife and biconcave
knife and can therefore be used for cutting harder materials. Because of thick nature
of wedge of the wedge at the tip, this knife cannot be ground be ground as sharp as
profile.
- The plane-wedge knife is usually 100mm in length. It has both sides straight,
recommended for frozen sections or for cutting extremely hard and tough
specimens embedded in paraffin blocks, using base sledge type or sliding
microtome. This type of knife is more stable and find use in hard materials,
such as in epoxy or cryogenic sample cutting. It is usually provided with backs,
to maintain the correct bevel angle throughout honing. Detachable handles
maybe attached to the knife during sharpening.

 Use: it is commonly used for cutting sections from paraffin wax embedded
material, frozen sections, cryostat sections and for small, synthetic resin
embedded material.
 Advantages:
- A wedge works by separating something apart. Some advantages of a wedge is that
it can take something apart that is really big and it is easier to move. Another is that
it is a fast way to get something apart.
 Disadvantages:
- Does not allow serial cuts, which slows down the process.
- Exposure of the blade can cause accidents.
- It is almost impossible to obtain sections with a thickness of less than 8 microns.

References:
Mohammed,F. et al. (2012). Microtomes and Microtome Knives: A review and
proposed classification. Retrieved on February 24, 2020 from
https//www.researchgate.net/publication/258221245_Microtomes_
and_Microtome_knives_A_Review_and_proposed_classification

SimpleMachines. (n,d). Wedge. Retrieved on February 24, 2020 from


https://meanjoegreen18simplemachines.weebly.com/wedge.html

Gregorios, J. H. ( 2017). Histopathologic Techniques. Miami Florida.

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