Oxford Cambridge and RSA

AS Level Biology A
H020/01 Breadth in biology

Thursday 26 May 2016 – Afternoon

Time allowed: 1 hour 30 minutes
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• a scientific calculator
• a ruler (cm/mm)

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First name

Last name

Centre Candidate
number number

INSTRUCTIONS
• Use black ink. You may use an HB pencil for graphs and diagrams.
• Complete the boxes above with your name, centre number and candidate number.
• Answer all the questions.
• Write your answer to each question in the space provided. If additional space is
required, you should use the lined page(s) at the end of this booklet. The question
number(s) must be clearly shown.
• Do not write in the barcodes.

INFORMATION
• The total mark for this paper is 70.
• The marks for each question are shown in brackets [ ].
• This document consists of 24 pages.

© OCR 2016 [601/4261/3]

DC (ST/SW) 123872/4 OCR is an exempt Charity Turn over
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SECTION A

You should spend a maximum of 25 minutes on this section.

Answer all the questions.

Write your answer for each question in the box provided.

1 Microscopes vary in their magnification and resolution.

Which of the rows, A to D, in the table below is correct?

Transmission electron Scanning electron

Light microscope
microscope microscope
Resolution Resolution Resolution
Magnification Magnification Magnification
(nm) (nm) (nm)
A × 1500 200 × 10 000 0.2 × 50 000 0.2
B × 400 100 × 500 000 10.0 × 100 000 0.2
C × 1500 200 × 500 000 0.2 × 100 000 0.2
D × 1500 100 × 500 000 10.0 × 100 000 10.0

Your answer [1]

2 Carbohydrates, such as starch, are made from monosaccharides joined together.

Which of the bonds, A to D, joins monosaccharides together?

A ester

B glycosidic

C peptide

D phosphodiester

Your answer [1]

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3 Some inorganic ions have roles in enzyme-controlled reactions.

Which of the rows, A to D, in the table below is correct?

Role of ion
Prosthetic group for
Cofactor for amylase
carbonic anhydrase
A Zn2+ Cl –
B Zn+ Cl –
C Cl 2– Zn+
D Cl – Zn2+

Your answer [1]

4 Which of the following statements, A to D, about the nature of the genetic code is incorrect?

A It is a degenerate code.

B It is a triplet code.

C It is overlapping.

D It is universal.

Your answer [1]

5 Ventilation involves various parts of the mammalian respiratory system.

Which of the following statements, A to D, describes inhalation?

A ribcage moves upwards and outwards; external intercostal muscles relax; diaphragm relaxes

B ribcage moves downwards and inwards; external intercostal muscles relax; diaphragm relaxes

C ribcage moves upwards and outwards; external intercostal muscles contract; diaphragm
contracts

D ribcage moves downwards and inwards; external intercostal muscles contract; diaphragm
contracts

Your answer [1]

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6 Which of the following structures, A to D, are found in prokaryotes and in eukaryotes?

A a cell wall made of peptidoglycan

B circular genomic DNA

C a nucleus surrounded by a nuclear membrane

D ribosomes

Your answer [1]

7 The pressure changes in one mammalian cardiac cycle are shown in the graph below.

Key
16
ventricular pressure
atrial pressure
12
pressure
(kPa)
8

0
0 0.2 0.4 0.6 0.8
time (s)

Which of the following time periods, A to D, shows ventricular systole?

A 0.0 to 0.1 s

B 0.2 to 0.3 s

C 0.4 to 0.5 s

D 0.6 to 0.8 s

Your answer [1]

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8 Measles is a serious disease that can be prevented by vaccination. The chart below shows the
Measles-containing Vaccine (MCV) coverage and annual reported cases of measles between
1980 and 2013.

Measles global annual reported cases and

MCV coverage, 1980 – 2013
5 000 000 100
4 500 000 90
4 000 000 80

MCV coverage (%)

number of cases

3 500 000 70
3 000 000 60
2 500 000 50
2 000 000 40
1 500 000 30
1 000 000 20
500 000 10
0 0
1980
1981
1982
1983
1984
1985
1986
1987
1988
1989
1990
1991
1992
1993
1994
1995
1996
1997
1998
1999
2000
2001
2002
2003
2004
2005
2006
2007
2008
2009
2010
2011
2012
2013
year
Key
number of cases MCV coverage

Which of the following statements, A to D, is a correct interpretation of the chart?

A An increase in herd immunity resulted in fewer deaths from measles.

B The highest number of measles cases occurred when MCV coverage was at its lowest.

C A 90% MCV coverage resulted in fewer than half a million cases of measles each year.

D There is a positive correlation between the number of measles cases and the MCV coverage.

Your answer [1]

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9 Two different fields, field G and H, were sampled for three common species of wildflower.
The results are shown below.

Number of individuals
Species Field G Field H
Daisy 300 20
Dandelion 335 49
Buttercup 365 931
Total 1000 1000

Which of the options, A to D, is correct?

A Field G will have a greater Simpson’s diversity index.

B Field H has greater species evenness.

C Field H will have a greater Simpson’s diversity index.

D Field G has greater species richness.

Your answer [1]

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10 Which of the following options, A to D, lists the three domains of life?

A Archaea, Bacteria and Eukaryota

B Bacteria, Prokaryota and Eukaryota

C Prokaryotae, Protoctista and Eukaryota

D Protoctista, Plantaea and Animalia

Your answer [1]

11 A student investigates some solutions, X, Y and Z, using paper chromatography.

The results are shown below.

solvent front

X Y Z

Which of the following options, A to D, is the Rf value of Z?

A 0.63

B 1.6

C 0.85

D 0.25

Your answer [1]

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12 Pressure varies in different parts of the mammalian circulatory system.

Blood in Blood in
Tissue fluid Lymph
aorta vena cava
Pressure

Which of the following options, A to D, correctly completes the table above?

A high high low low

B high low high low

C high low low low

D high low low high

Your answer [1]

13 A diagram of a potometer is shown below.

screw clip

air bubble

Which of the following options, A to D, is a precaution that is not needed when setting up a
potometer?

A Remove excess water from the surface of the leaves before readings are taken.

B The screw clip must be opened while taking the readings.

C The shoot should be cut whilst under water.

D There should be no extra air bubbles.

Your answer [1]

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14 The image shows a stage in mitosis.

Which of the following options, A to D, is the stage of mitosis shown above?

A anaphase

B metaphase

C prophase

D telophase

Your answer [1]

15 The diagram below shows the simplified structure of an antibody.

D C

Which of the letters, A to D identifies the region of the antibody that allows the distance between
the antibody binding sites to vary.

Your answer [1]

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16 A group of students were investigating the diffusion of molecules across membranes using a
‘model cell’, as shown below.

‘model cell’

‘extracellular fluid’

‘cytoplasm’ (solution
containing starch
and amylase)

Biochemical tests were used to identify the types of molecules present. The results are shown in
the table below.

A tick (3) represents a positive result.

Which of the rows, A to D, shows the correct results for the ‘cytoplasm’ at the beginning of the
experiment and the ‘extracellular fluid’ at the end of the experiment?

Benedict’s test Biuret test Iodine test

‘extracellular ‘extracellular ‘extracellular
‘cytoplasm’ ‘cytoplasm’ ‘cytoplasm’
fluid’ fluid’ fluid’
A 3 3 3
B 3 3 3 3
C 3 3 3 3
D 3 3 3

Your answer [1]

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17 Which of the cells below, represented by cubes A to D, has a surface area to volume ratio of 3 : 1 ?

6 μm

4 μm

2 μm
1 μm

nucleus
nucleus
nucleus nucleus

A B C D

Your answer [1]

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18 A diagram of a stained blood smear observed under a light microscope is shown below.

Which of the structures labelled A to D in the diagram is a neutrophil?

Your answer [1]

19 Which of the following options, A to D, is a primary defence mechanism against pathogens?

A neutralisation

B agglutination

C phagocytosis

D blood clotting

Your answer [1]

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20 Pathogens cause disease and are transmitted from individual to individual in a variety of ways.

Which of the rows, A to D, in the table below is correct?

Type of
Disease Means of transmission
pathogen
A Athlete’s foot Fungus Direct and indirect contact
B HIV/AIDs Virus Indirect contact
C Malaria Bacterium Vector
D Tuberculosis Protoctist Direct contact

Your answer [1]

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SECTION B

Answer all the questions.

21 Fig. 21.1 shows some of the apparatus used in an experiment investigating water potential in
potato tuber tissue.

boiling tube

sucrose solution

discs of potato tuber tissue

Fig. 21.1

The discs were placed in boiling tubes containing sucrose solutions of different concentrations for
four hours. The percentage change of mass was then calculated.

The results are shown in Table 21.2.

Concentration of sucrose solution Change in mass of

(mol dm–3) potato discs (%)
0.00 +18.00
0.10 +12.50
0.20 + 2.50
0.30 – 3.00
0.40 – 8.00
0.45 –11.50

Table 21.2

(a) (i) State two details of the procedure that must be followed to obtain valid results.

1 ........................................................................................................................................

...........................................................................................................................................

2 ........................................................................................................................................

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[2]

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(ii) Explain how a student could use the data in Table 21.2 to determine the water potential
of the potato tuber tissue.

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(b) Fig. 21.3 shows a diagram of a cell from a potato tuber disc that was placed in 0.45 mol dm–3
sucrose solution.

W X
Y

nucleus
U

Fig. 21.3

(i) Identify the parts of the cell labelled X, Y and Z.

X ...............................................................

Y ...............................................................

Z ................................................................
[3]

(ii) What will be found at W in the immersed cell?

...................................................................................................................................... [1]

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(c) Halophytes are plants that have the ability to live in soils with a very low water potential. In the
UK these plants form part of salt marsh communities.

Suggest and explain how the root hairs of halophytes are able to absorb water by osmosis
from the soil of the salt marsh.

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22 Even the smallest DNA molecules are very long.
• A kilobase (Kb) is a unit equivalent to 1000 base pairs of a DNA molecule.
• One Kb of double stranded DNA has a length of 0.34 µm.

(a) The DNA in the nucleus of a cell from a fruit fly (Drosophila) is 5.6 cm long.

(i) Calculate the number of Kb in the DNA of the fruit fly.

Show your working. Give your answer to the nearest whole number.

Answer = .................................................... Kb [2]

(ii) The DNA of the fruit fly was analysed and 22% of the bases were adenine.

What % of the bases were guanine? Show your working.

Answer = ..................................................... % [2]

(b) A DNA molecule contains polynucleotide strands.

(i) Individual nucleotides are joined together to make a polynucleotide strand.

What type of chemical reaction takes place when two nucleotides in a single
polynucleotide strand are joined together?

...................................................................................................................................... [1]

(ii) Name the chemical released when the bond is formed between the two nucleotides.

...................................................................................................................................... [1]

(iii) A DNA molecule contains two polynucleotide chains.

Describe how these two chains are held together.

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23 The Sumatran rhinoceros, Dicerorhinus sumatrensis, is a rare member of the family Rhinocerotidae.
These rhinoceros are now critically endangered, with only six substantial populations in the wild:
four in Sumatra, one in Borneo, and one in the Malay Peninsula.

D. sumatrensis lives in rainforests. Their numbers are difficult to determine but they are estimated
to number fewer than 100.

(a) Complete the table below, showing the classification of the Sumatran rhinoceros.

Kingdom ............................................................................

Phylum Chordata

............................................................................ Mammalia

............................................................................ Perissodactyla

Family Rhinocerotidae

............................................................................ Dicerorhinus

Species ............................................................................

[2]

(b) The name Dicerorhinus sumatrensis is part of the binomial naming system.

State an advantage of using this system rather than using the common name, Sumatran
rhinoceros.

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(c) (i) Suggest two reasons why this species is critically endangered in the wild.

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(ii) The remaining populations of D. sumatrensis are all small and are scattered in isolated
areas. These are factors that might speed up the extinction of the species.

Suggest why.

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(iii) Captive breeding programmes with D. sumatrensis have been unsuccessful.

Suggest one other way in which zoos can contribute to the conservation of the Sumatran
rhinoceros.

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24 In mammalian blood, oxygen is mainly transported combined with haemoglobin. The presence of
haemoglobin greatly increases the oxygen carrying capacity of blood.

• 100 cm3 of plasma contains 0.3 cm3 of oxygen when fully saturated.

• 100 cm3 of blood contains 20.1 cm3 of oxygen when fully saturated.

(a) Calculate the percentage increase in oxygen carried in fully saturated blood compared with
oxygen carried in fully saturated plasma.

Show your working.

Answer = ..................................................... % [2]

(b) Haemoglobin is found in erythrocytes. Unlike other vertebrates, the mature erythrocytes of
mammals lack nuclei and other membrane-bound organelles.

(i) Explain one advantage and one disadvantage of the lack of nuclei and other membrane-
bound organelles to mammalian erythrocytes.

Advantage .........................................................................................................................

...........................................................................................................................................

Disadvantage ....................................................................................................................

...................................................................................................................................... [2]

(ii) Viruses do not use erythrocytes as host cells, whereas the malarial pathogen Plasmodium
spends part of its life cycle inside erythrocytes.

Suggest why.

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(iii) Explain why erythrocytes do not make use of any of the oxygen that they are transporting.

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(c) Oxygenated blood returns from the lungs to the heart before being pumped around the body.

(i) • Normal cardiac output is 5 dm3 min–1.

• 100 cm3 of blood contains 20.1 cm3 of oxygen when fully saturated.

Calculate the volume (cm3) of oxygen being transported to the tissues per minute.

Show your working and give your answer to four significant figures.

Answer = .................................................. cm3 [2]

(ii) With reference to the structure of blood vessels, explain why oxygen is not released until
the blood reaches the capillaries.

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(d) Fig. 24 shows the oxygen dissociation curves at different carbon dioxide concentrations.

100

80 low pCO2

60 high pCO2
% saturation
of haemoglobin
40

20

0
0 2 4 6 8 10 12 14
partial pressure
T of oxygen (kPa)

Fig. 24

(i) What name is given to a change in the oxygen dissociation curve due to increasing
carbon dioxide concentration?

...................................................................................................................................... [1]

(ii) Letter T in Fig. 24 indicates the partial pressure of oxygen in actively respiring tissues.

Explain why the blood off-loads more oxygen to actively respiring tissues than to resting
tissues.

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25 The following statements summarise the results from experiments designed to discover more
about the translocation of organic materials in the phloem.

Any increase in the sugar content of leaves is followed by a similar change in the sieve
A
tube contents in the stem.
The rate of transport increases with increasing temperature, reaching a maximum at
B
25 °C before decreasing at higher temperatures.

C Translocation stops when stems are treated with a substance that inhibits respiration.

D Sugars can be transported both up and down the plant.

E Aphids can be used to sample phloem sap.

F Roots, young leaves and growing fruits will import sugars.

(a) State all the letters that provide evidence for the following conclusions:

Translocation is an active process.

...................................................................................................................................................

Sugars are translocated from source to sink.

...................................................................................................................................................
[4]

(b) Explain how mass flow of the phloem sap occurs in plants with a vascular system.

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END OF QUESTION PAPER

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ADDITIONAL ANSWER SPACE

If additional space is required, you should use the following lined page(s). The question number(s)
must be clearly shown in the margin.

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